Method for improving yield of wormwood, extract and application of extract

Abstract

The invention provides a method for increasing the yield of wormwood, an extract and application of the extract, and belongs to the technical field of traditional Chinese medicine, according to the method for increasing the yield of the wormwood, the extract and the application of the extract, in the aspect of planting, the yield of the wormwood is increased through environment optimization, good variety selection and scientific management, and in the extraction process, the yield of the wormwood is increased. Comprising the steps of raw material pretreatment, solvent soaking, concentration treatment, purification operation, finished product drying and the like. Ethanol is used for solvent soaking, the concentration and the temperature are controlled, and soaking is performed twice; carrying out vacuum concentration twice at specific temperature and pressure in the concentration treatment; impurities are removed through column chromatography or gel filtration and other methods in the purification operation, and the extract can be applied to the fields of medicine, beauty and skin care, health care and the like, can effectively relieve symptoms such as rheumatic arthritis and muscular soreness and further has the effects of resisting bacteria, diminishing inflammation, resisting oxidation and the like.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a method for improving the yield of wormwood, an extract and application of the extract. Background Art

[0002] As a traditional Chinese medicinal material, mugwort has a wide range of medicinal value. However, there are some problems in the existing cultivation and extraction process of mugwort, resulting in a low yield of mugwort and unstable quality of the extract. In terms of cultivation, factors such as the growth environment, variety selection, and planting management measures of mugwort have an important impact on the yield and quality of mugwort. However, in the current planting process, there are problems such as improper selection of the planting environment, poor variety, and unscientific planting management, which affect the yield of mugwort. In terms of extraction, the existing extraction methods may have problems such as low extraction efficiency, large loss of effective ingredients, and incomplete removal of impurities, resulting in the quality and purity of the extract being difficult to meet market demand.

[0003] Therefore, based on the above technical problems, it is necessary to design a method for improving the yield of wormwood and an extract as well as the application of the extract. The yield of wormwood can be improved by optimizing the planting environment and variety selection, and improving planting and management measures. At the same time, the use of a specific extraction process, including raw material pretreatment, solvent soaking, concentration treatment, purification operation and drying of the finished product, can effectively improve the quality and purity of the extract, so that it can be better used in medicine, beauty and skin care, health care and other fields. Summary of the invention

[0004] The purpose of the present invention is to provide a method for improving the yield of mugwort, an extract and the application of the extract, aiming to solve the problems of low extraction efficiency, large loss of effective ingredients, incomplete removal of impurities, etc. in the extraction method of the prior art, which makes it difficult for the quality and purity of the extract to meet market demand.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] The method for improving the yield of wormwood, the extract and the application of the extract comprise the following steps:

[0007] S1. Raw material pretreatment: Select high-quality, dry, pest-free and low-impurity wormwood to ensure its good quality. Wash the wormwood carefully to remove dust, dirt and other impurities on the surface. Place the washed wormwood in a well-ventilated place to dry the moisture, cut and crush the dried wormwood into small sections to facilitate subsequent crushing operations. Use professional crushing equipment to crush the wormwood sections to 50-200 meshes to fully break the cell structure of the wormwood to facilitate the dissolution of the effective ingredients.

[0008] S2, solvent soaking: select ethanol as the extraction solvent, the ethanol concentration is controlled at 70%-90%, put the crushed wormwood powder into a soaking container, add an appropriate amount of ethanol to completely immerse the wormwood, soak for the first time at room temperature (20-25°C) for 18-24 hours, filter after soaking, transfer the soaking liquid to a storage tank, retain the wormwood residue, add ethanol to the wormwood residue again, soak for the second time, the time and conditions are the same as the first time, filter the second soaking liquid, and combine it with the first soaking liquid;

[0009] S3, concentration treatment: the combined soaking liquid is placed in a vacuum concentration device, and the first vacuum concentration is performed at an appropriate temperature (40-60° C.) and pressure to concentrate the volume of the soaking liquid to 1 / 5-1 / 10 of the original volume to obtain a first concentrated liquid, the first concentrated liquid is collected, and the second vacuum concentration is continued to further increase the concentration of the concentrated liquid;

[0010] S4. Purification operation: purify the concentrate by column chromatography or gel filtration, select appropriate chromatography columns and gel media, separate and purify according to the properties of the active ingredients in the wormwood extract, remove impurities and ineffective ingredients, and dry the finished product. Dry the purified solution, and vacuum drying or spray drying can be used. When vacuum drying, control the temperature at 50-70°C and the pressure at 0.05-0.1MPa until a dry powdered wormwood extract is obtained. When spray drying, adjust the inlet temperature to 150-200°C and the outlet temperature to 80-100°C, spray the solution into fine droplets, and quickly dry to obtain the extract powder.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] 1. In this plan, the yield of mugwort is improved: by optimizing the planting environment and variety selection, improving the planting and management measures, such as strictly selecting high-quality mugwort in raw material pretreatment, and performing fine cleaning and crushing operations, laying the foundation for improving the yield of mugwort. In the extraction process, two solvent soakings are used to maximize the extraction of effective ingredients. Through reasonable concentration treatment, the concentration of effective ingredients can be further increased, thereby improving the yield of mugwort.

[0013] 2. In this scheme, the quality and purity of the extract are improved: in the solvent immersion, ethanol is selected as the extraction solvent and the concentration is controlled at 70%-90%, which is conducive to the dissolution of the active ingredients and avoids excessive dissolution of impurities. In the purification operation, column chromatography or gel filtration and other methods are used. According to the properties of the active ingredients, suitable chromatography columns and gel media are selected to effectively remove impurities and ineffective ingredients and improve the purity of the extract. In the stage of drying the finished product, both vacuum drying and spray drying have strict temperature and pressure control to ensure that the active ingredients are not destroyed during the drying process, thereby ensuring the quality of the extract. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0015] Figure 1 The present invention is a flow chart of the preparation method of the mugwort extract. DETAILED DESCRIPTION

[0016] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] Example 1

[0018] See also Figure 1 , the present invention provides the following technical solutions:

[0019] The method for improving the yield of wormwood, the extract and the application of the extract comprise the following steps:

[0020] S1. Raw material pretreatment: Select high-quality, dry, pest-free and low-impurity wormwood to ensure its good quality. Wash the wormwood carefully to remove dust, dirt and other impurities on the surface. Place the washed wormwood in a well-ventilated place to dry the moisture, cut and crush the dried wormwood into small sections to facilitate subsequent crushing operations. Use professional crushing equipment to crush the wormwood sections to 50-200 meshes to fully break the cell structure of the wormwood to facilitate the dissolution of the effective ingredients.

[0021] S2, solvent soaking: select ethanol as the extraction solvent, the ethanol concentration is controlled at 70%-90%, put the crushed wormwood powder into a soaking container, add an appropriate amount of ethanol to completely immerse the wormwood, soak for the first time at room temperature (20-25°C) for 18-24 hours, filter after soaking, transfer the soaking liquid to a storage tank, retain the wormwood residue, add ethanol to the wormwood residue again, soak for the second time, the time and conditions are the same as the first time, filter the second soaking liquid, and combine it with the first soaking liquid;

[0022] S3, concentration treatment: the combined soaking liquid is placed in a vacuum concentration device, and the first vacuum concentration is performed at an appropriate temperature (40-60° C.) and pressure to concentrate the volume of the soaking liquid to 1 / 5-1 / 10 of the original volume to obtain a first concentrated liquid, the first concentrated liquid is collected, and the second vacuum concentration is continued to further increase the concentration of the concentrated liquid;

[0023] S4. Purification operation: purify the concentrate by column chromatography or gel filtration, select appropriate chromatography columns and gel media, separate and purify according to the properties of the active ingredients in the wormwood extract, remove impurities and ineffective ingredients, and dry the finished product. Dry the purified solution, and vacuum drying or spray drying can be used. When vacuum drying, control the temperature at 50-70°C and the pressure at 0.05-0.1MPa until a dry powdered wormwood extract is obtained. When spray drying, adjust the inlet temperature to 150-200°C and the outlet temperature to 80-100°C, spray the solution into fine droplets, and quickly dry to obtain the extract powder.

[0024] In a specific embodiment of the present invention, S1, raw material pretreatment: In the raw material selection link, we adhere to strict standards and carefully select those wormwoods with excellent growth environment and just the right maturity. High-quality wormwood usually comes from unpolluted production areas and receives sufficient sunlight and nutrients during the growth process. In order to ensure its dryness, the moisture content will be measured by professional testing equipment. Only when the moisture content is lower than a certain standard can it be identified as dry wormwood. The inspection of pests and impurities is even more meticulous. Experienced staff will remove any problematic wormwood through naked eye observation and the assistance of simple tools. During the cleaning process, it is rinsed multiple times with running clean water to ensure that every grain of dust, every piece of soil and other impurities that may be attached to the surface of the wormwood are thoroughly removed, and the intensity and time of the rinse are carefully controlled. The system ensures that the mugwort is cleaned and avoids unnecessary damage to the mugwort. When drying the moisture, choose a well-ventilated, clean and dust-free environment. The racks or platforms for placing the mugwort must be disinfected to prevent the mugwort from being contaminated again during the drying process. At the same time, pay attention to controlling the speed and direction of ventilation so that the mugwort can be dried evenly to avoid local moisture causing mold. When cutting, use sharp and sterilized knives to cut the mugwort into uniform small segments. This is not only convenient for subsequent crushing operations, but also helps to ensure the consistency of the crushing effect. During the crushing process, professional crushing equipment will operate at an appropriate speed and power to ensure that the mugwort can be evenly crushed to 50-200 mesh. At the same time, the temperature and humidity inside the equipment will also be monitored to prevent the heat and humidity changes generated by friction from affecting the effective ingredients of the mugwort.

[0025] S2. Solvent soaking: The choice of ethanol as the extraction solvent is a deliberate one. Ethanol has good solubility and penetration, and can effectively extract the active ingredients in wormwood. Its concentration is controlled at 70%-90%. This is a range determined after many experiments and optimizations. Within this concentration range, the active ingredients can be fully dissolved while avoiding the dissolution of too many impurities. When the crushed wormwood powder is placed in the soaking container, the powder must be evenly distributed to avoid accumulation and agglomeration. The amount of ethanol added must be accurately calculated based on the amount of wormwood to ensure that the wormwood is completely immersed to achieve full contact and extraction. The first soaking is carried out at room temperature (20-25°C). This temperature range is conducive to maintaining the activity and stability of ethanol, and can also avoid the destruction of the active ingredients by excessive temperature. The soaking time of 8-24 hours is to allow the effective ingredients to fully dissolve. During this period, the environment should be kept quiet and stable to avoid vibration and temperature fluctuations from interfering with the soaking process. After the soaking, a fine and suitable filter screen is used to ensure that the effective ingredients remain in the soaking solution and the residue is effectively separated. The process of transferring the soaking solution to the storage tank should be rapid and avoid leakage and contamination. For the wormwood residue after the first soaking, ethanol with the same concentration and conditions is added again for a second soaking in order to extract the remaining effective ingredients to the greatest extent. The time and conditions of the second soaking are the same as those of the first soaking to ensure the consistency of the extraction effect. Finally, the second soaking solution is filtered and combined with the first soaking solution to provide a sufficient solution rich in effective ingredients for subsequent processing steps.

[0026] S3. Concentration treatment: Before the combined soaking liquid is placed in the vacuum concentration equipment, the equipment will be strictly cleaned and disinfected to prevent any possible contamination from affecting the quality of the concentrate. The operating parameters of the vacuum concentration equipment, including temperature and pressure, are precisely debugged and set. The temperature is selected in the range of 40-60°C, which is determined based on the thermal stability of the active ingredients and the evaporation characteristics of the solvent. In this temperature range, the active ingredients can be protected from destruction while the solvent can evaporate rapidly. The pressure setting is to create an environment conducive to the rapid volatilization of the solvent, while avoiding excessive vacuum causing violent boiling of the solution or other instability. During the first vacuum concentration During the process, the volume change of the concentrate will be continuously monitored, and its volume will be concentrated to 1 / 5-1 / 10 of the original volume through precise metering devices. This ratio is controlled to increase the concentration of the active ingredient while avoiding over-concentration that will cause difficulties in subsequent processing. When collecting the first concentrate, use a sterile, impurity-free container and avoid excessive contact with the outside air during the collection process to prevent oxidation or contamination. The second reduced pressure concentration is to further refine the first concentrate to achieve higher concentration requirements. This process also requires strict control of temperature, pressure and degree of concentration to ensure that the final concentrate has stable quality and high purity.

[0027] S4. Purification operation: When performing purification operations, the selection of methods such as column chromatography or gel filtration is based on a deep understanding of the physicochemical properties of the active ingredients in the wormwood extract. The selection of chromatographic columns and gel media is crucial, and factors such as pore size, adsorption performance and chemical stability need to be considered. For example, for active ingredients with larger molecular weights, gel media with larger pore sizes will be selected to ensure that they can pass smoothly and be separated from impurities. During the separation and purification process, the composition and flow rate of the mobile phase will be adjusted according to the polarity, charge and other properties of the active ingredients. Through continuous testing and optimization, the best separation conditions are found to maximize the removal of impurities and ineffective ingredients while ensuring the recovery rate of the active ingredients. In the stage of drying the finished product, vacuum drying and spray drying are used. It has its advantages. During vacuum drying, the temperature is controlled at 50-70℃ and the pressure is controlled at 0.05-0.1MPa in order to quickly remove moisture while ensuring that the active ingredients are not damaged by heat. This temperature and pressure range has been verified by many experiments and can ensure the efficiency and stability of the drying process. During spray drying, the inlet temperature is adjusted to 150-200℃ and the outlet temperature is 80-100℃ in order to quickly atomize the solution into fine droplets and evaporate the water in a short time. The higher inlet temperature can quickly dry the droplets, while the relatively low outlet temperature helps to avoid overheating and deterioration of the dried powder. During the entire spray drying process, parameters such as temperature and flow rate are closely monitored to ensure that the obtained extract powder has good particle size distribution and stability.

[0028] For details, please refer to Figure 1 , wormwood extract can effectively relieve symptoms such as rheumatoid arthritis and muscle soreness.

[0029] In this embodiment: the wormwood extract also has antibacterial and anti-inflammatory properties, and can be used to treat infectious diseases caused by various bacteria and fungi. In the field of beauty and skin care, its antioxidant effect can delay skin aging, reduce wrinkles and spots, its antibacterial and acne-removing effect can improve acne-prone skin, and its skin-soothing effect can reduce allergies and inflammation. In the field of health care, it can enhance immunity and regulate endocrine, especially for some physiological problems of women. Rheumatoid arthritis and muscle soreness are common diseases, which bring great pain and inconvenience to patients.

[0030] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for improving the yield of wormwood, an extract and an application of the extract, characterized in that: The following steps are involved: S1. Raw material pretreatment: Select high-quality, dry, pest-free and low-impurity wormwood to ensure its good quality. Wash the wormwood carefully to remove dust, dirt and other impurities on the surface. Place the washed wormwood in a well-ventilated place to dry the moisture, cut and crush the dried wormwood into small sections to facilitate subsequent crushing operations. Use professional crushing equipment to crush the wormwood sections to 50-200 meshes to fully break the cell structure of the wormwood to facilitate the dissolution of the effective ingredients. S2, solvent soaking: select ethanol as the extraction solvent, the ethanol concentration is controlled at 70%-90%, put the crushed wormwood powder into a soaking container, add an appropriate amount of ethanol to completely immerse the wormwood, soak for the first time at room temperature (20-25°C) for 18-24 hours, filter after soaking, transfer the soaking liquid to a storage tank, retain the wormwood residue, add ethanol to the wormwood residue again, soak for the second time, the time and conditions are the same as the first time, filter the second soaking liquid, and combine it with the first soaking liquid; S3, concentration treatment: the combined soaking liquid is placed in a vacuum concentration device, and the first vacuum concentration is performed at an appropriate temperature (40-60° C.) and pressure to concentrate the volume of the soaking liquid to 1 / 5-1 / 10 of the original volume to obtain a first concentrated liquid, the first concentrated liquid is collected, and the second vacuum concentration is continued to further increase the concentration of the concentrated liquid; S4. Purification operation: purify the concentrate by column chromatography or gel filtration, select appropriate chromatography columns and gel media, separate and purify according to the properties of the active ingredients in the wormwood extract, remove impurities and ineffective ingredients, and dry the finished product. Dry the purified solution, and vacuum drying or spray drying can be used. When vacuum drying, control the temperature at 50-70°C and the pressure at 0.05-0.1MPa until a dry powdered wormwood extract is obtained. When spray drying, adjust the inlet temperature to 150-200°C and the outlet temperature to 80-100°C, spray the solution into fine droplets, and quickly dry to obtain the extract powder.

2. The use of the wormwood extract according to claim 1 in preparing medicines, characterized in that: Mugwort extract can effectively relieve symptoms such as rheumatoid arthritis and muscle aches.

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