Ointment for radiodermatitis, preparation method and external medicine
By using ointments with ingredients such as cockroaches such as the American cockroach, the existing problems of toxicity, difficulty in obtaining and high price for the treatment of acute radiodermatitis ointment are solved, and the effects of reducing symptoms, promoting repair, reducing toxicity and improving the convenience of acquisition are achieved.
Patent Information
- Application Number
- CN202411879959.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-19
- Publication Date
- 2025-04-18
AI Technical Summary
The existing ointments for treating acute radiodermatitis have problems such as high toxicity risks, high difficulty in obtaining and high price.
The ointment with ingredients such as American cockroach dry insect body, white scutellaria baicalensis, scutellaria baicalensis, angelica, licorice and beeswax is prepared through ultrasonic extraction and beeswax melting, reducing toxicity and improving the convenience of acquisition.
This ointment significantly reduces the symptoms of radiodermatitis, promotes the repair of skin wounds, improves the speed of skin recovery in rats, and reduces toxicity, is convenient to obtain, and is relatively low in price.
Smart Images
Figure CN119837972A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of radiation dermatitis, and in particular to an ointment for radiation dermatitis, a preparation method and an external medicine. Background Art
[0002] With the rapid development of nuclear industry and radiomedicine, the role of radiotherapy in the treatment of malignant tumors has become increasingly evident. More than 75% of cancer patients will receive radiotherapy at a certain stage of the occurrence and development of the disease, and up to 95% of patients will experience varying degrees of radiation skin damage. In addition to routine care, Western medicine lacks recognized effective drugs for preventing and alleviating radiation dermatitis. In recent years, there have been many clinical reports on the prevention and treatment of radiation dermatitis with significant efficacy in traditional Chinese medicine. After years of research, people have found that traditional Chinese medicines that have the functions of clearing away heat and detoxifying, promoting blood circulation and removing blood stasis, replenishing blood and qi, and nourishing yin and raising whiteness all have varying degrees of anti-radiation effects, and traditional Chinese medicines have a wide source of medicine and low toxicity, which makes them show great advantages and potential in the research of radiation protective agents.
[0003] Radiation dermatitis is the most common complication during radiotherapy. Severe radiation dermatitis has a great impact on the patient's quality of life. Patients feel worried, frustrated and depressed because of skin symptoms. Radiation dermatitis is an inflammatory skin lesion caused by various types of ionizing radiation (such as X-rays, beta rays, gamma rays, particle rays, etc.). According to the time of occurrence of radiation dermatitis, acute radiation dermatitis occurs within a few days or 3 months after radiation, and chronic radiation dermatitis occurs 3 months to several years after radiation exposure. Patients with acute radiation dermatitis, mild cases show skin pigmentation, hair follicle dilation, pain, itching, dry desquamation, and erythema; severe cases have symptoms such as edema, ulcers, bleeding, infection, etc., which leads to interruption of radiotherapy and prolonged treatment cycle.
[0004] Radiation dermatitis can be classified as "burns" in traditional Chinese medicine. In the Qing Dynasty, Wu Shiji said in "Lihua Pianwen": "The principle of external treatment is the same as the principle of internal treatment, and the medicine for external treatment is also the medicine for internal treatment. The only difference is the method... All effective soups and pills can be boiled into ointments." "Outline of Ulcers" also said: "Ulcers are diseases that manifest themselves externally, so external treatment medicines are particularly important; for mild symptoms, relying solely on external treatment can achieve complete results; but for dangerous and large ulcers, it is especially necessary to use external treatment appropriately. The two methods are used interchangeably, which is the science of ulcer medicine." Therefore, in clinical practice, traditional Chinese medicine often uses external Chinese medicine to treat acute radiation dermatitis, with the principles of clearing away heat and detoxification, nourishing yin and promoting tissue regeneration, and cooling blood and activating blood circulation.
[0005] The existing Chinese medicine treatment mostly uses Shengji Yuhong ointment. Shengji Yuhong ointment is a traditional Chinese medicine ointment for treating trauma and skin problems. It is said to be from the book "Surgery Authenticity" by Chen Shigong, a doctor in the Ming Dynasty. The main ingredients of this ointment include Chinese herbs such as calomel, licorice, angelica, angelica, lithospermum, and white wax.
[0006] In Shengji Yuhong Paste, light powder mainly contains mercurous chloride (Hg2Cl2). Its external use has a certain stimulating effect on the skin and mucous membranes, can coagulate local proteins, inhibit bacterial growth and reproduction, and thus play a bactericidal and anti-inflammatory role. Its toxic effect is mainly that after mercury ions enter the human body, they combine with the sulfhydryl groups of various enzymes in the body, interfere with cell metabolism, and damage multiple systems such as the nervous system, digestive system, and urinary system. Long-term or excessive use can lead to mercury poisoning, with a series of poisoning symptoms such as oral mucosal ulcers, nausea, vomiting, abdominal pain, diarrhea, and proteinuria.
[0007] Secondly, it is difficult to obtain insect wax. It is a wax secreted by male waxworms parasitizing on the branches of Oleaceae plants such as privet trees or fraxinus trees. First of all, its production process is complicated and requires careful cultivation of waxworms, including accurate grasp of the stocking time and appropriate stocking density. If the time or density is wrong, it will affect the secretion of wax. Secondly, the output is limited by many factors, and the climate factor is crucial. Suitable temperature, humidity and light are necessary conditions for the growth and secretion of wax by waxworms. Extreme weather such as drought or heavy rain will have an adverse effect on it, and pests and diseases will also threaten the survival of waxworms and reduce wax production. Finally, since the quality varies depending on the origin, collection and processing methods, high-quality insect wax requires white color, hard texture, and no impurities, which makes it more difficult to obtain high-quality insect wax.
[0008] In summary, existing prescriptions for the treatment of acute radiation dermatitis have the problems of high toxicity risks, high difficulty in obtaining, and high prices. Summary of the invention
[0009] The purpose of the present invention is to solve the deficiencies in the prior art, to provide a mild and easily available ointment and a preparation method for patients with radiation dermatitis, and to provide an external medicine for radiation dermatitis.
[0010] In order to solve the above technical problems, the technical method adopted by the present invention is as follows: the present invention discloses an ointment for radiation dermatitis, the composition of the ointment includes: 15g of dried American cockroach body, 20g of white schizonepeta, 15g of scutellaria, 10g of lithospermum officinale, 15g of angelica dahurica, 50g of angelica sinensis, 0.5g of blood clotting, 30g of liquorice, and 50g of beeswax.
[0011] The present invention also discloses a method for preparing an ointment for radiation dermatitis, comprising the following steps:
[0012] S1. Cut the dried bodies of American cockroaches, Bletilla striata, Scutellaria baicalensis, Lithospermum officinale, Angelica dahurica, Chinese angelica and Licorice into small pieces, add sesame oil and stir to soak;
[0013] S2. Ultrasonic extraction was performed at 55°C, and filtration was performed after extraction.
[0014] S3. The extract is concentrated to an appropriate concentration to obtain an initial ointment;
[0015] S4. Take natural beeswax, heat it to 60°C, filter it after it is fully melted, pour the ointment into it during stirring, mix it thoroughly and continue stirring;
[0016] S5. Take the mixed solution, add the Dragon's Blood powder into the mixed solution, stir it at 60°C and cool it naturally to obtain the finished ointment.
[0017] Furthermore, in step S1, 50 g of angelica sinensis, 30 g of liquorice, 20 g of bletilla striata, 15 g of angelica dahurica, 15 g of scutellaria baicalensis, 15 g of dried American cockroach bodies, and 10 g of lithospermum officinale are selected, cut into pieces, and 30 ml of sesame oil is added per gram of the medicinal materials for stirring and soaking.
[0018] Furthermore, in the step S2, ultrasonic extraction is performed at 55° C. for 1 hour, and the mixture is filtered after extraction to obtain a first filtrate.
[0019] Furthermore, the filter residue obtained after the extraction is taken, and the filter residue is extracted once in 30KHz ultrasound for 0.5h, and after standing and filtering, it is combined with the first extract for use.
[0020] Furthermore, in step S4, 50 g of natural beeswax is taken, heated to 60° C., and filtered after being fully melted.
[0021] Furthermore, in the step S4, the melted beeswax and the initial ointment are fully mixed and stirred for 30 minutes to obtain a mixed solution.
[0022] Furthermore, in the step S5, 0.5 g of Dragon's Blood powder is added to the mixed solution.
[0023] Furthermore, in step S5, 0.5 g of Dragon's Blood powder is added to the mixed solution, and the mixture is stirred at 60° C. for 30 minutes and then naturally cooled to obtain a finished ointment.
[0024] The present invention also discloses an external medicine for preventing and treating radiation dermatitis, comprising the above ointment for radiation dermatitis or the ointment prepared by the above ointment preparation method for radiation dermatitis, and the ointment can be prevented and treated by continuous application, so as to achieve the effect of reducing skin damage and promoting repair.
[0025] Beneficial effects:
[0026] This ointment is derived from the Shengji Yuhong Ointment in "The Authentic Surgery", in which calomel is removed to reduce the toxicity of the ointment, and cockroach and beeswax are used instead of insect wax. While the efficacy is similar, the raw materials are easy to obtain, protecting the resources of traditional Chinese medicine, and adding white radix and scutellaria baicalensis to enhance the effect of clearing heat and healing sores.
[0027] The ointment uses American cockroach and Bletilla striata as the main ingredients. The cockroach can promote blood circulation and remove blood stasis, while Bletilla striata can astringe and stop bleeding, reduce swelling and promote tissue regeneration. The one dispersing and the other astringing can promote tissue regeneration, reduce swelling and heal sores. The ointment uses Scutellaria baicalensis and Lithospermum officinale as the auxiliary ingredients. Scutellaria baicalensis can clear away heat and detoxify, while Lithospermum officinale can detoxify and reduce swelling, thus clearing away heat, detoxifying and reducing swelling. The ointment uses Angelica dahurica, Chinese angelica and Sanguisorba officinalis as the auxiliary ingredients. Angelica dahurica can discharge pus and relieve pain, while Chinese angelica can promote blood circulation and nourish blood, promote tissue regeneration and relieve pain, thus relieving pain, while Sanguisorba officinalis can disperse blood stasis, relieve pain, stop bleeding and promote tissue regeneration. The ointment uses Licorice as the messenger, which can clear away heat and detoxify and harmonize the various ingredients. BRIEF DESCRIPTION OF THE DRAWINGS
[0028] Figure 1 The following are histopathological microscopic images of skin sections of rats in the sham operation group, model group and ointment group in the specific embodiments of the present invention. DETAILED DESCRIPTION
[0029] The present invention can be better understood according to the following examples. However, it is easy for those skilled in the art to understand that the specific material ratios, process conditions and results described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.
[0030] A medicinal ointment for treating radiation dermatitis comprises: 15g of dried American cockroach bodies, 20g of white schizonepeta, 15g of scutellaria, 10g of lithospermum officinale, 15g of angelica dahurica, 50g of angelica sinensis, 0.5g of dragon's blood, 30g of liquorice and 50g of beeswax.
[0031] In this recipe, the dried body of American cockroach
[0032] Medicinal properties: sweet, warm. Return to the lung and spleen meridians.
[0033] Efficacy: Relieve cough and resolve phlegm, replenish qi and tonify deficiency, promote blood circulation and remove blood stasis, and have anti-tumor effect.
[0034] Application: Used for chronic cough due to lung deficiency, shortness of breath and wheezing, liver and stomach qi stagnation, spleen deficiency diarrhea, auxiliary treatment of cancer, etc.
[0035] Pharmacology: American cockroaches contain a variety of active ingredients, such as peptides and amino acids, which have immune-enhancing and anti-tumor effects. Studies have found that they can enhance the self-repair ability of the lungs and have a certain effect on inhibiting the growth of tumor cells.
[0036] White and
[0037] Medicinal properties: bitter, flat. Return to the lung and spleen meridians.
[0038] Efficacy: Relieve cough and resolve phlegm, reduce swelling and relieve pain, promote blood circulation and remove blood stasis.
[0039] Application: Used for chronic cough, excessive sputum, weak lung qi, tuberculosis, swelling and pain relief, used for carbuncles, sores, lumps, etc.
[0040] Pharmacology: The active ingredients in Bletilla striata have anti-inflammatory and hemostatic effects and can promote wound healing. Experiments have shown that Bletilla striata has an inhibitory effect on bacterial infections, especially for certain chronic coughs, tuberculosis and other diseases.
[0041] Scutellaria baicalensis
[0042] Medicinal properties: bitter, cold. Return to the lung, gallbladder, and stomach meridians.
[0043] Efficacy: Clears away heat and dampness, purges fire and detoxifies, stops bleeding, and is anti-allergic.
[0044] Application: Used for lung heat cough, sore throat, dysentery, eczema, jaundice, etc.
[0045] Pharmacology: The flavonoids in Scutellaria baicalensis have antibacterial, antiviral and anti-inflammatory effects. Studies have shown that Scutellaria baicalensis has strong antioxidant and anti-cancer effects, especially in the treatment of liver diseases and skin diseases.
[0046] Lithospermum officinale
[0047] Medicinal properties: sweet, cold. Regulates the heart and liver meridians.
[0048] Efficacy: Promote blood circulation and remove blood stasis, detoxify and reduce swelling, moisturize the skin and promote tissue regeneration.
[0049] Application: Used to treat blood stasis pain, sores, swelling, trauma, burns, etc.
[0050] Pharmacology: Lithospermum officinale contains active ingredients such as shikonin and shikoninol, which have antibacterial, antiviral and detoxification effects. It is often used for external treatment of skin diseases, burns and scalds, and recovery from acute trauma.
[0051] Angelica dahurica
[0052] Medicinal properties: pungent, warm. Return to the lung, spleen and stomach meridians.
[0053] Efficacy: Induces sweat and relieves exterior symptoms, dispels wind and relieves pain, reduces swelling and discharges pus, and opens the orifices.
[0054] Application: used for wind-cold-dampness arthralgia, headache, toothache, nasal congestion, cough, carbuncle, sore, etc. Commonly used for upper respiratory tract infection, head and facial pain and other diseases.
[0055] Pharmacology: Angelica dahurica has antibacterial, anti-inflammatory and anti-allergic effects, and can relieve headaches, nasal congestion and other symptoms caused by cold. Its volatile oil components have analgesic and anti-inflammatory effects.
[0056] Angelica
[0057] Medicinal properties: sweet, pungent, warm. Enter the heart, liver, and spleen meridians.
[0058] Efficacy: Nourishes blood and promotes blood circulation, regulates menstruation and relieves pain, moisturizes the intestines and promotes bowel movements.
[0059] Application: Used for pale complexion, palpitations, insomnia, irregular menstruation, dysmenorrhea, bruises, dry intestines and constipation caused by blood deficiency.
[0060] Pharmacology: The volatile oil components in angelica have anti-thrombotic and microcirculation-improving effects. Many studies have shown that angelica has the effect of enhancing red blood cell production and improving blood circulation, especially for irregular menstruation and postpartum recovery.
[0061] Dragon's Blood
[0062] Medicinal properties: sweet, warm. Entrance to the heart, liver, spleen meridians.
[0063] Efficacy: Promote blood circulation and remove blood stasis, stop bleeding, reduce swelling and relieve pain.
[0064] Application: Used to treat traumatic bleeding, bruises, carbuncles, sores, dysmenorrhea, etc.
[0065] Pharmacology: The main ingredients in dragon's blood, such as dragon's acid, have a strong hemostatic effect and can promote blood circulation. They have significant therapeutic effects on promoting tissue repair and inhibiting bacterial growth.
[0066] Licorice
[0067] Medicinal properties: sweet, neutral. Enters the heart, lung, spleen and stomach meridians.
[0068] Efficacy: tonifies the spleen and replenishes Qi, clears away heat and detoxifies, removes phlegm and relieves cough, relieves pain, and harmonizes various medicines.
[0069] Application: Used for spleen and stomach weakness, fatigue, palpitations and shortness of breath, cough with sputum, acute pain in the abdomen and limbs, carbuncles and sores, and to relieve the toxicity and potency of drugs.
[0070] Pharmacology: Glycyrrhizic acid has an inhibitory effect on Oberling Guerin myeloma transplanted in rats, and is also effective when taken orally. Glycyrrhizin and Licorice root can produce morphological changes in rat ascites liver cancer and mouse Ehrlich ascites cancer cells. Glycyrrhizin can also inhibit subcutaneously transplanted Yoshida sarcoma.
[0071] beeswax
[0072] Medicinal properties: sweet, warm. Return spleen, lung, liver meridians.
[0073] Efficacy: nourishes the lungs and relieves cough, resolves phlegm and softens hard masses, reduces swelling and relieves pain, moistens the intestines and promotes bowel movements, nourishes the stomach and strengthens the spleen.
[0074] Application: It is used for cold cough, phlegm and asthma, sore throat, dry intestines and constipation, wound ulcers, etc. It is often used for cough, chest tightness, phlegm, etc. caused by lung and spleen deficiency.
[0075] Pharmacology: There are some studies on the effects of beeswax on cell membranes, antioxidant effects, anti-inflammation, and promotion of tissue regeneration. Experiments have shown that beeswax has antioxidant and immune system-enhancing functions, and has a certain effect on relieving respiratory diseases and repairing damaged tissues.
[0076] Example 1: Preparation method of ointment for anti-radiation dermatitis
[0077] This example describes in detail the preparation method of the ointment for radiation dermatitis, including the selection, extraction, mixing of medicinal materials and the preparation of the final product.
[0078] The ointment is composed of ingredients such as the dried body of American cockroaches, Bletilla striata, Scutellaria baicalensis, Lithospermum officinale, Angelica dahurica, Chinese angelica, licorice and beeswax.
[0079] 1. Raw material preparation
[0080] The main ingredients of this ointment include:
[0081] 15g dried American cockroach body, 20g Bletilla striata, 15g Scutellaria baicalensis, 10g Lithospermum officinale, 15g Angelica dahurica, 50g Chinese angelica, 30g Licorice, 50g Beeswax, 0.5g Sanguisorba officinalis.
[0082] 2. Soaking and extraction of medicinal materials
[0083] Step S1: Cut the dried bodies of American cockroaches, Bletilla striata, Scutellaria baicalensis, Lithospermum officinale, Angelica dahurica, Chinese angelica and Licorice into small pieces, and add 30 ml of sesame oil per gram of the medicinal materials.
[0084] Step S2: Using a 20KHz ultrasonic extractor to ultrasonically extract the soaked medicinal material at 55°C for 1 hour. After extraction, filtering is performed to obtain a first filtrate.
[0085] Step S3: extract the residue again in a 30KHz ultrasonic extractor for 0.5 hours, let it stand and filter to obtain a second filtrate. Combine the filtrates obtained from the two extractions and concentrate them to an appropriate concentration to obtain an initial ointment.
[0086] 3. Melt beeswax and mix with ointment
[0087] Step S4: Take 50 g of natural beeswax, heat it to 60° C., and filter out impurities to ensure that the beeswax is pure.
[0088] Step S5: Mix the melted beeswax and the extract, and stir them thoroughly for 30 minutes to ensure that the beeswax is evenly dissolved in the ointment to obtain a uniform ointment base.
[0089] 4. Adding Dragon's Blood Powder and Final Product Preparation
[0090] Step S6: In step S5, add 0.5 g of Dragon's Blood powder, and continue stirring at 60° C. for 30 minutes to ensure that the Dragon's Blood is completely dissolved and fully integrated with the ointment.
[0091] Step S7: After the stirring is completed, the ointment is cooled naturally to obtain a final ointment product, which is in a uniform paste state and suitable for external use.
[0092] Example 2: Ointment intervention experiment for radiation dermatitis
[0093] An animal experiment was conducted to verify the therapeutic effect of the ointment prepared in Example 1 on radiation dermatitis. The experiment irradiated rats with electron rays (β rays) generated by an electron accelerator to observe the effect of the ointment in the treatment of radiation dermatitis.
[0094] 1. Experimental equipment and animal models
[0095] Instruments and equipment: A linear accelerator produced by Elekta Limited, UK, was used for radiation exposure experiments. Self-made lead box: The base is composed of a stainless steel box with a length of 25 cm, a width of 25 cm, and a height of 8 cm. The inside is divided into four small spaces with a width of 6 cm by iron sheets (normally able to accommodate a rat (excluding the tail)). The top cover is composed of a 3 mm thick lead plate (wrapped with a layer of iron sheet). A circular hole with a diameter of 3 cm is cut near one end to expose the rat skin and form a radioactive exposure area.
[0096] Animal selection and grouping: 30 healthy male SPF SD rats weighing 180–200 g were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. All rats were adaptively fed for one week before the experiment to ensure that their health conditions met the experimental requirements. The rats were randomly divided into three groups, with 10 rats in each group:
[0097] Sham operation group (control group): used to observe normal skin reactions, without radiation exposure, only simulated surgery.
[0098] Model group (radiation dermatitis group): radiation exposure was performed but no ointment was applied, in order to observe the skin damage caused by radiation.
[0099] Ointment group (experimental group): The mice were irradiated and the ointment prepared in Example 1 was applied to observe the therapeutic effect of the ointment.
[0100] The rats were raised in cages, 5 in each cage, with free access to food and water. The feed was SPF rat and mouse feed produced by Beijing Keao Xieli Feed Co., Ltd. The temperature in the breeding room was controlled at 20-25°C, and the humidity was controlled at 40-70%. The breeding environment was strictly controlled to ensure the health of the rats.
[0101] 2. Experimental Methods
[0102] Radiation injury: Electron beams (β-rays) were generated by an electron accelerator and irradiated to the skin on the back of the rat's buttocks. Before irradiation, the skin on the back of the rat's buttocks was shaved and the irradiation dose was 40 Gy. The occurrence of radiation dermatitis was simulated by irradiation.
[0103] Among them, the specific modeling method is that 15 minutes before the electron accelerator electron beam (β ray) irradiation, the rat's buttocks and back skin is shaved (the shaved area is greater than 10cm2), and after skin preparation, it is anesthetized with Staxtal 50. After anesthesia, the animal is placed in a homemade lead box (3mm thick, with a circular hole with a diameter of 3cm in the buttocks and back area of the animal), and then a marker is used to draw a line on the animal's skin along the circular hole, which is the radiation irradiation area; the lead box is placed under the electron accelerator irradiation field, and an Elekta Synergy linear accelerator is used, 6Mev, dose rate, 6Gy / min, that is, the total irradiation dose is 40Gy.
[0104] Ointment application: After radiation injury, the rats in the ointment group were smeared with the ointment prepared in the experiment according to the standardized protocol. The application amount was 0.5 g each time, the application frequency was 2 times a day, and the application lasted for 4 weeks.
[0105] 3. Observation and evaluation
[0106] General status observation: The skin damage of rats in the ointment group and the model group was observed at the 2nd, 3rd and 6th week after radiotherapy.
[0107] Ointment group: In the second week, the skin of the rats in the ointment group had only slight erythema and peeling, but no damage. In the third week, the skin of the rats in the ointment group had slight scabs, and in the sixth week, the skin of the rats basically returned to normal.
[0108] Model group: The rats in the model group had skin damage and scabs in the second week, exudation appeared in the third week, and the skin of some rats had not completely healed in the sixth week.
[0109] Wound area measurement: The wound areas of rats were measured at the 1st, 2nd, 3rd and 6th weeks. The results are shown in the following table:
[0110] Group Week 1 Week 2 Week 3 Week 6 Sham operation group - - - - Model Group - <![CDATA[1.5*2.2cm 2 ]]> <![CDATA[2.8*3.1cm 2 ]]> <![CDATA[1.2*1.5cm 2 ]]> Ointment Group - <![CDATA[1.0*1.2cm 2 ]]> <![CDATA[1.3*2.5cm 2 ]]> -
[0111] Statistical analysis: The wound area of rats in the ointment group was significantly smaller than that in the model group, and the recovery speed was faster. Statistical analysis showed that the ointment group had a significant advantage in wound repair (p<0.01).
[0112] 4. Histopathological Examination
[0113] HE staining examination: At the 6th week after radiotherapy, histopathological examination was performed to observe the skin tissue structure of rats in the ointment group and the model group.
[0114]
[0115]
[0116] Figure 1 The results of histopathological examination of the skin sections of rats in the sham operation group, model group and ointment group are shown. As shown by the HE results, the cells of the epidermis of the skin of rats in the sham operation group were clearly arranged, and the structure of the skin appendages was intact.
[0117] 60 days after irradiation, the number of sebaceous glands and hair follicles in the skin appendages of the rats in the model group was significantly reduced, the skin epidermis was thickened, accompanied by increased keratinization or incomplete keratinization, lymphocyte infiltration, and granulation tissue hyperplasia.
[0118] The number of sebaceous glands and hair follicles in the skin of rats in the ointment group was reduced to a certain extent and had not yet returned to normal levels, but there was no obvious abnormality in the skin epidermis, no incomplete keratinization in the stratum corneum, and no obvious lymphocyte infiltration.
[0119] Compared with the model group, the skin in the ointment group recovered more rapidly, the epidermal structure was more complete, the stratum corneum and skin appendages recovered better, and there was no obvious lymphocyte infiltration.
[0120] 5. Experimental Results
[0121] The experimental data and pathological analysis showed that the ointment of the present invention can significantly alleviate the symptoms of radiation dermatitis, promote the repair of skin wounds, and increase the skin recovery rate of rats. The effect of the ointment group in the treatment of radiation dermatitis was significantly better than that of the model group, especially in the histopathological examination, the ointment group showed less inflammatory response and better skin repair status.
[0122] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.
Claims
1. An ointment for radiation dermatitis, characterized in that: The ointment comprises: 15g of dried American cockroach body, 20g of Bletilla striata, 15g of Scutellaria baicalensis, 10g of Lithospermum officinale, 15g of Angelica dahurica, 50g of Angelica sinensis, 0.5g of Sanguisorba officinalis, 30g of Licorice root and 50g of Beeswax.
2. A method for preparing an ointment for radiation dermatitis, characterized in that: The following steps are involved: S1. Cut the dried bodies of American cockroaches, Bletilla striata, Scutellaria baicalensis, Lithospermum officinale, Angelica dahurica, Chinese angelica and Licorice into small pieces, add sesame oil and stir to soak; S2. Ultrasonic extraction was performed at 55°C, and filtration was performed after extraction. S3. The extract is concentrated to an appropriate concentration to obtain an initial ointment; S4. Take natural beeswax, heat it to 60°C, filter it after it is fully melted, pour the ointment into it during stirring, mix it thoroughly and continue stirring; S5. Take the mixed solution, add the Dragon's Blood powder into the mixed solution, stir it at 60°C and cool it naturally to obtain the finished ointment.
3. The method for preparing an ointment for radiation dermatitis according to claim 2, characterized in that: In step S1, 50 g of Chinese angelica, 30 g of liquorice, 20 g of white twig, 15 g of angelica dahurica, 15 g of scutellaria baicalensis, 15 g of dried American cockroach, and 10 g of lithospermum officinale are selected, cut into pieces, and 30 ml of sesame oil is added per gram of the medicinal materials for stirring and soaking.
4. The method for preparing an ointment for radiation dermatitis according to claim 3, characterized in that: In the step S2, ultrasonic extraction is performed at 55° C. for 1 hour, and the mixture is filtered after extraction to obtain a first filtrate.
5. The method for preparing an ointment for radiation dermatitis according to claim 4, characterized in that: The filtered residue after the extraction is taken, and the filter residue is extracted once in 30KHz ultrasound for 0.5h, and after standing and filtering, it is combined with the first extract for use.
6. The method for preparing an ointment for treating radiation dermatitis according to any one of claims 2 to 3, characterized in that: In the step S4, 50 g of natural beeswax is taken, heated to 60° C., and filtered after being fully melted.
7. The method for preparing the ointment for radiation dermatitis according to claim 6, characterized in that: In the step S4, the melted beeswax and the initial ointment are fully mixed and stirred for 30 minutes to obtain a mixed solution.
8. The method for preparing the ointment for radiation dermatitis according to claim 7, characterized in that: In the step S5, 0.5 g of Dragon's Blood powder is added to the mixed solution.
9. The method for preparing the ointment for radiation dermatitis according to claim 7, characterized in that: In the step S5, 0.5 g of Dragon's Blood powder is added to the mixed solution, and the mixture is stirred at 60° C. for 30 minutes and then naturally cooled to obtain a finished ointment.
10. An external-use medicine for preventing and treating radiation dermatitis, characterized in that: The invention discloses an ointment for radiation dermatitis according to claim 1 or an ointment prepared by the preparation method of the ointment for radiation dermatitis according to claims 2 to 9, and the ointment can be used for prevention and treatment by continuous application, so as to achieve the effect of alleviating skin damage and promoting repair.