Method for treating pig farm wastewater by using direct ammonia oxidizing bacteria

Abstract

The invention belongs to the technical field of high-concentration wastewater treatment, and discloses a method for treating pig farm wastewater by using direct ammonia oxidizing bacteria. Comprising the following steps: resuscitating and activating direct ammonia oxidizing bacteria; growth and culture of direct ammonia oxidizing bacteria; the actual pig farm wastewater is directly treated by ammonia oxidizing bacteria; and starting the MBBR reactor by taking direct ammonia oxidizing bacteria as an inoculum and the simulated pig farm wastewater as inlet water. The invention aims to provide a method for treating swine wastewater through direct ammoxidation. The method has strong tolerance to high-concentration ammonia nitrogen, has good denitrification performance, simplifies the process flow and reduces the operation cost.

Description

Technical Field

[0001] The invention relates to the technical field of livestock and poultry breeding wastewater treatment, and in particular to a method for directly ammonia-oxidizing piggery wastewater. Background Art

[0002] With the adjustment of my country's rural industrial structure, livestock and poultry farming, especially pig farming, has developed rapidly, and pig wastewater has also been produced in large quantities, which has brought great pressure to the ecological environment. Pig wastewater is mainly composed of feces, urine, feed residues, and pen flushing water. It is characterized by high concentrations of organic pollutants, nitrogen-containing substances, and suspended solids with a large fluctuation range. Improper treatment of pig wastewater and the release of its pollutants into the environment will cause damage to water bodies, soil, air and other environments and threaten the health of related animals and humans. Therefore, in order to protect the ecological environment and reduce the pollution caused by it, the denitrification technology of pig farm wastewater is extremely important.

[0003] The treatment of piggery wastewater at home and abroad usually adopts physical and chemical methods as pretreatment process, and the secondary biochemical treatment adopts anaerobic, anoxic and aerobic combined process. The difficulty of piggery wastewater treatment lies in the inhibitory effect of high concentration of ammonia nitrogen on microbial growth, and the traditional wastewater treatment process is too long and the operating cost is high. Therefore, the sustainable development of the pig industry and environmental protection urgently need an efficient and low-carbon piggery wastewater treatment method. Summary of the invention

[0004] In order to overcome the shortcomings of the prior art, the present invention aims to provide a method for treating piggery wastewater by direct ammonia oxidation, in view of the inhibitory effect of high-concentration ammonia nitrogen in the prior art of piggery wastewater, the long process flow of traditional wastewater treatment and the high operating costs. The method has a strong tolerance to high-concentration ammonia nitrogen, has good denitrification performance, simplifies the process flow and reduces operating costs.

[0005] The above object of the present invention is achieved through the following technical solutions:

[0006] A method for treating piggery wastewater by direct ammonia oxidation comprises: resuscitation and activation of strains with direct ammonia oxidation function; growth and cultivation of strains with direct ammonia oxidation function; treating actual piggery wastewater by direct ammonia oxidation; starting an MBR reactor using strains with direct ammonia oxidation function as inoculants and simulated piggery wastewater as influent.

[0007] Furthermore, the strain with direct ammonia oxidation function is obtained by purchasing Alcaligenes faecalis subsp. faecalis from the China General Microbiological Culture Collection Center (CGMCC), numbered CGMCC1.1837, which has direct ammonia oxidation function.

[0008] Furthermore, the resuscitation and activation of the strain with direct ammonia oxidation function is specifically as follows: preparing a nutrient broth agar medium, the specific ingredients of which are as follows: 5.0g of peptone, 3.0g of beef extract, 5.0g of NaCl, 1000ml of distilled water, pH 7.0-7.5. The above medium is sterilized at 121-126°C for 20-30min, and the purchased strain CGMCC1.1837 is transferred to the above medium on a clean bench to revive the strain.

[0009] Furthermore, the culture conditions for the growth and cultivation of the strain with direct ammonia oxidation function are: ammonia nitrogen concentration of 400-3000 mg / L, C / N of 5-20, salinity of 0.0%-2.0%, pH of 7.0-9.0, carbon source of CH3COONa, and temperature of 29.5-30.5°C.

[0010] Furthermore, direct ammonia oxidation treatment of actual pig farm wastewater is specifically as follows: the pig farm wastewater is untreated raw water from a pig farm in Shandong, the obtained pig farm wastewater is filtered, the filtered pig farm wastewater is sterilized at 121-126°C for 20-30 minutes, the above-mentioned strain CGMCC1.1837 is inoculated on the clean bench, sealed with a sealing film, and three parallel sets are set up.

[0011] Furthermore, the inoculated actual pig wastewater was placed in a constant temperature oscillator. The constant temperature oscillation conditions were a temperature culture temperature of 29.5-30.5°C, a rotation speed of 150-160rpm, a cycle of 7-9d, and a water quality test every 23-24h.

[0012] Furthermore, the influent of the MBR reactor uses simulated pig farm wastewater: (NH4)2SO4 is used as the nitrogen source and CH3COONa is used as the carbon source. During the startup phase of the MBR reactor, the water quality is as follows: NH4 + -N concentration is 1000-1500mg / L, COD concentration is 7000-10000mg / L.

[0013] As a further step, during the start-up of the MBR reactor, CGMCC1.1837 was used as the inoculum, where the concentration of CGMCC1.1837 bacterial solution was OD 600 The volume ratio of CGMCC1.1837 bacterial solution to simulated pig wastewater is 1 / 25. The reaction temperature is maintained at 29-31°C by a water bath heating layer. The MBR reactor is operated under aeration conditions, providing oxygen to maintain bacterial activity and carrier movement, and the aeration volume is controlled at a rate of 1.0-1.8L / min by a flow meter. The hydraulic retention time (HRT) is set to 4-6 days. When NH4 +The removal of -N and COD tended to be stable and a stable biofilm was formed on the carrier, indicating that the MBR reactor was successfully started.

[0014] The present invention also seeks to protect the application of the above method for direct ammonia oxidation treatment of piggery wastewater in treating piggery wastewater.

[0015] The beneficial effects of the present invention compared with the prior art are:

[0016] Compared with the existing pig wastewater treatment technology, the advantages of the present invention are: 1) the growth rate of the strain with direct ammonia oxidation function is significantly higher than that of autotrophic nitrifying bacteria, and it is easier to proliferate and enrich; 2) it can maintain high activity in the pH range of 7-10; 3) it does not require anaerobic and anoxic stages, which greatly simplifies the process flow, greatly reduces space requirements, and reduces operating costs; 4) it has strong tolerance to high ammonia nitrogen in pig wastewater; 5) it can maintain high activity in a wide C / N range (5-20), and effectively denitrify and remove carbon from pig wastewater simultaneously. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a performance comparison diagram of four strains with direct ammonia oxidation function; Figure 1 a is the denitrification effect of the four strains within 48 h; Figure 1 b shows the growth of the four strains within 48 h.

[0018] Figure 2 Flowchart for the growth culture of CGMCC1.1837.

[0019] Figure 3 These are the effects diagrams of CGMCC1.1837 treating actual pig farm wastewater, specifically, the denitrification efficiency diagram of CGMCC1.1837 treating actual pig farm wastewater and the carbon removal effect diagram of CGMCC1.1837 treating actual pig farm wastewater.

[0020] Figure 4 Schematic diagram of the MBR reactor structure.

[0021] In the figure: 1 constant temperature water tank; 2 first water inlet pump; 3 MBR reaction tank; 4 stirring propeller motor; 5 stirring propeller blades; 6 aeration head; 7 second water inlet pump; 8 water inlet tank. DETAILED DESCRIPTION

[0022] Below in conjunction with the embodiment of the present invention, the technical scheme of the present invention is clearly and completely described, it is obvious that the described embodiment is only a part of the embodiment of the present invention, rather than all the embodiments. Based on the embodiment in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work premise belong to the scope of protection of the present invention. Unless otherwise specified, the equipment and reagents used in the present invention are conventional commercial products in the art.

[0023] The term "including" and its variations used in the present invention represent open inclusion, i.e., "including but not limited to". The terms "first", "second", etc. may refer to different or the same objects. Other explicit and implicit definitions may also be included below.

[0024] The present embodiment provides a method for treating pig farm wastewater with a strain having direct ammonia oxidation function, comprising: resuscitation and activation of the strain having direct ammonia oxidation function; growth and cultivation of the strain having direct ammonia oxidation function; direct ammonia oxidation treatment of actual pig farm wastewater; starting an MBR reactor using the strain having direct ammonia oxidation function as an inoculant and simulated pig farm wastewater as influent.

[0025] Example 1

[0026] (1) Resuscitation and activation of strains with direct ammonia oxidation function

[0027] Alcaligenes faecalis subsp. faecalis was purchased from the China General Microbiological Culture Collection Center (CGMCC), numbered CGMCC1.1837, which has direct ammonia oxidation function. The method for resuscitation and activation of strains with direct ammonia oxidation function is as follows: according to the instructions attached to the Culture Collection Center, nutrient broth agar medium is prepared, and the specific ingredients are as follows: 5.0g peptone, 3.0g beef extract, 5.0g NaCl, 1000ml distilled water, pH 7.0-7.5. The above culture medium is sterilized at 121°C for 30min, and the purchased strain CGMCC1.1837 is transferred to the above culture medium on a clean bench for strain resurrection.

[0028] (2) Growth and cultivation of strains with direct ammonia oxidation function

[0029] The strain CGMCC1.1837 was grown and cultured under the following conditions: ammonia nitrogen concentration of 500 mg / L, C / N of 10, salinity of 0.2%, pH of 7.0, carbon source of CH3COONa, and temperature of 30°C. Specific culture process:

[0030] S1: Prepare culture medium / L: (NH4)2SO42.36 g, CH3COONa 6.41 g, KH2PO40.44 g, MgSO4.7H2O0.2 g, NaCl 2 g, trace elements 2.00 ml, pH 7.0. Take 100 ml of the culture medium into a 250 ml conical flask, seal it with sealing film, set up three parallels for each group, and sterilize it in a high pressure steam sterilizer at 121°C for 30 minutes.

[0031] S2: When the temperature of the high-pressure steam sterilizer in step S1 drops to about 30°C, take out the culture medium in it, place it on the clean bench, turn on the ultraviolet sterilization lamp, perform ultraviolet sterilization on the inside of the clean bench for 30 minutes, turn on the air supply switch of the clean bench, and inoculate 1 ml of CGMCC1.1837 bacterial solution into the above culture medium, where the bacterial solution concentration OD 600 is 2.0232.

[0032] S3: Place the culture medium inoculated with CGMCC1.1837 in step S2 in a constant temperature oscillator at a culture temperature of 30°C and a rotation speed of 150 rpm. Take samples on the clean bench every 24 hours for a period of 4 days and measure the water quality index.

[0033] (3) Direct ammonia oxidation treatment of actual piggery wastewater

[0034] The piggery wastewater was taken from untreated raw water from a piggery in Shandong. The basic water quality indicators of the piggery wastewater were as follows: ammonia nitrogen content 1200-1500 mg / L, total nitrogen 1300-1600 mg / L, COD content 8000-8500 mg / L, and pH value 7.4-7.6. The piggery wastewater was filtered through a 0.45um water system microporous filter membrane using a vacuum filter. The piggery wastewater after filtration was dark brown and free of other impurities. Filtration could remove most of the suspended matter. The filtered piggery wastewater was divided into 100 ml in a 250 ml conical flask, and three parallel bottles were set up. Sterilized at 121°C for 30 minutes, and 2 ml of the aforementioned CGMCC1.1837 bacterial solution was inoculated into the sterilized piggery wastewater, where the bacterial solution concentration OD 600 The concentration of ammonia nitrogen and COD in the wastewater was determined to determine the denitrification effect.

[0035] Depend on Figure 3 It can be seen that direct ammonia oxidation can achieve efficient denitrification and carbon removal of pig farm wastewater, with an ammonia nitrogen removal rate of 84.16% and a total organic carbon removal rate of 89.6%.

[0036] (4) Start the MBR reactor with a strain with direct ammonia oxidation function as inoculum

[0037] Wastewater treatment was carried out in a cylindrical MBR reactor made of organic glass with a height of 30 cm, a diameter of 12 cm, and an effective volume of 3.0 L. Figure 4As shown, the MBR reactor includes: a constant temperature water tank, a first water inlet pump, an MBR reaction tank, a stirring paddle motor, a stirring paddle blade, an aeration head, a second water inlet pump, and a water inlet tank. The constant temperature water tank, the first water inlet pump, the MBR reaction tank, the second water inlet pump, and the water inlet tank are connected in sequence, and the stirring paddle motor, the stirring paddle blade, and the aeration head are connected to the MBR reaction tank. The stirring paddle motor is located at the top of the MBR reaction tank, the stirring paddle blade is located inside the MBR reaction tank, and the aeration head is located at the bottom of the MBR reaction tank. The top of the stirring paddle blade is connected to the stirring paddle motor.

[0038] The constant temperature water tank 1 continuously supplies water to the water bath insulation layer of the MBR reaction tank 3 through the first water supply pump 2 to control the temperature in the MBR reactor to be constant. The simulated piggery wastewater in the water inlet tank 8 enters the MBR reaction tank 3 under the action of the second water inlet pump 7, and is fully mixed with CGMCC1.1837 in the MBR reaction tank 3 under the action of the stirring blade 5. Two aeration heads 6 are installed at the bottom of the MBR reactor to provide dissolved oxygen DO for the MBR reactor.

[0039] The influent of the MBR reactor is simulated pig farm wastewater: (NH4)2SO4 is used as the nitrogen source and CH3COONa is used as the carbon source. During the startup phase of the MBR reactor, the water quality is as follows: NH4 + -N concentration is 1000-1500mg / L, COD concentration is 7000-10000mg / L. During the startup of the MBR reactor, CGMCC1.1837 is used as the inoculum, and the CGMCC1.1837 bacterial solution concentration OD 600 The volume ratio of CGMCC1.1837 bacterial solution to simulated pig wastewater is 1 / 25. The reaction temperature is maintained at 30°C by a water bath heating layer. The MBR reactor is operated under aeration conditions, providing oxygen to maintain bacterial activity and carrier movement, and the aeration volume is controlled at a rate of 1.0-1.8L / min by a flow meter. The hydraulic retention time (HRT) is set to 5d. OD is regularly tested. 600 , MLSS, TN removal rate, COD removal rate and NH4 + -N removal rate, when NH4 + The removal of -N and COD tended to be stable and a stable biofilm was formed on the carrier, indicating that the MBR reactor was successfully started.

[0040] Example 2

[0041] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: the ammonia nitrogen concentration was 400 mg / L, and the rest was the same as in Example 1.

[0042] Example 3

[0043] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: the ammonia nitrogen concentration was 600 mg / L, and the rest was the same as in Example 1.

[0044] Example 4

[0045] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: the ammonia nitrogen concentration was 800 mg / L, and the rest was the same as in Example 1.

[0046] Example 5

[0047] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: the ammonia nitrogen concentration was 1000 mg / L, and the rest was the same as in Example 1.

[0048] Example 6

[0049] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: the ammonia nitrogen concentration was 1500 mg / L, and the rest was the same as in Example 1.

[0050] Example 7

[0051] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: the ammonia nitrogen concentration was 2000 mg / L, and the rest was the same as in Example 1.

[0052] Example 8

[0053] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: the ammonia nitrogen concentration was 3000 mg / L, and the rest was the same as in Example 1.

[0054] Example 9

[0055] In the step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: C / N was 5, and the rest were the same as in Example 1.

[0056] Example 10

[0057] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: C / N was 20, and the rest were the same as in Example 1.

[0058] Embodiment 11

[0059] In the step (2) of growing and culturing the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultured, and the culture conditions were: the salinity was 0.0%, and the rest were the same as in Example 1.

[0060] Example 12

[0061] In the step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated. The culture conditions were: the salinity was 0.4%, and the other conditions were the same as in Example 1.

[0062] Embodiment 13

[0063] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated. The culture conditions were: the salinity was 0.6%, and the other conditions were the same as in Example 1.

[0064] Embodiment 14

[0065] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated. The culture conditions were: the salinity was 0.8%, and the other conditions were the same as in Example 1.

[0066] Embodiment 15

[0067] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated. The culture conditions were: the salinity was 1.0%, and the other conditions were the same as in Example 1.

[0068] Example 16

[0069] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated. The culture conditions were: the salinity was 2.0%, and the other conditions were the same as in Example 1.

[0070] Embodiment 17

[0071] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: pH 7.0, and the rest were the same as in Example 1.

[0072] Embodiment 18

[0073] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: pH 8.0, and the rest were the same as in Example 1.

[0074] Embodiment 19

[0075] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated. The culture conditions were as follows: pH 9.0, and the rest were the same as in Example 1.

[0076] Comparative Example 1

[0077] In step S1, a strain with direct ammonia oxidation function is selected from the resuscitation and activation of the strain with direct ammonia oxidation function, and Alcaligenes faecalis subsp. faecalis is purchased from the China General Microbiological Culture Collection Center (CGMCC), with the number CGMCC1.2098. Others are the same as step S1 in Example 1.

[0078] Comparative Example 2

[0079] In step S1, a strain with direct ammonia oxidation function is selected from the resuscitation and activation of the strain with direct ammonia oxidation function, and a water-producing alkali bacteria with the number CGMCC1.9051 is purchased from the China General Microbiological Culture Collection Center (CGMCC). Others are the same as step S1 of Example 1.

[0080] Comparative Example 3

[0081] In step S1, a strain with direct ammonia oxidation function is selected from the resuscitation and activation of the strain with direct ammonia oxidation function, and Alcaligenes faecalis is purchased from the China Industrial Microbiological Culture Collection (CICC), with the number CICC2.3439. Others are the same as step S1 of Example 1.

[0082] Comparative Example 4

[0083] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: the ammonia nitrogen concentration was 3500 mg / L, and the rest was the same as in Example 1.

[0084] Comparative Example 5

[0085] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: the ammonia nitrogen concentration was 4000 mg / L, and the rest was the same as in Example 1.

[0086] Comparative Example 6

[0087] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: the ammonia nitrogen concentration was 5000 mg / L, and the rest was the same as in Example 1.

[0088] Comparative Example 7

[0089] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: C / N was 1, and the rest were the same as in Example 1.

[0090] Comparative Example 8

[0091] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated. The culture conditions were: C / N was 2.5, and the rest were the same as in Example 1.

[0092] Comparative Example 9

[0093] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated. The culture conditions were: the salinity was 3.0%, and the other conditions were the same as in Example 1.

[0094] Comparative Example 10

[0095] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: pH 5, and the rest were the same as in Example 1.

[0096] Comparative Example 11

[0097] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were: pH 6, and the rest were the same as in Example 1.

[0098] Comparative Example 12

[0099] In step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were as follows: the carbon source was glucose, the ammonia nitrogen concentration was 140 mg / L, and the rest was the same as in Example 1.

[0100] Comparative Example 13

[0101] In the step (2), in the growth and cultivation of the strain with direct ammonia oxidation function, the strain CGMCC1.1837 was grown and cultivated, and the culture conditions were as follows: the carbon source was starch, the ammonia nitrogen concentration was 140 mg / L, and the rest was the same as in Example 1.

[0102] Performance study of strains with direct ammonia oxidation function: The performance of the revived strains of Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 was studied; Heterotrophic nitrification medium (HNM / L) was prepared: (NH4)2SO40.66g, CH3COONa 9.44g, KH2PO40.5g, MgSO4.7H2O1.25g, NaCl 10.0g, trace elements 2.00ml (trace elements: EDTA·2Na 57.10g, ZnSO4·7H2O 3.90g, CaCl2·2H2O 7.00g, MnCl2.4H2O 1.00g, FeSO4·7H2O 5.00g, (NH4)6Mo7O 24 ·4H2O 1.10g, CuSO4·5H2O 1.60g, CoCl2·6H2O 1.60g, pH 6.0), pH 7.0-7.5). Take 100ml of the culture medium and place it in a 250ml conical flask. Sterilize the culture medium at 121-126°C for 30min. Take 1ml of the bacterial solution of each of the four strains revived in Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 and inoculate it into the culture medium. The initial OD of the culture medium is 600 The values ​​were all around 0.03, sealed with sealing film, and three parallels were set in each group, all placed in a constant temperature oscillator. The temperature was 30°C, the speed was 150rpm, the cycle was 2d, and the water quality index was measured every 24h to measure the OD of the bacterial solution. 600 The value is used to determine the growth of the bacteria, and the concentration of ammonia nitrogen in the wastewater is measured to determine the denitrification effect.

[0103] Depend on Figure 1 a It can be seen that the removal efficiency of the strain CGMCC1.1837 can reach 73.41% within 24 hours, and the ammonia nitrogen in the culture medium can be completely removed within 48 hours. Figure 1 b It can be seen that after 48 hours of cultivation, the bacterial concentration of CGMCC1.1837 was significantly higher than that of other strains, indicating that the proliferation ability of this strain was relatively good. Therefore, CGMCC1.1837 showed good denitrification performance throughout the process.

[0104] The above description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is obvious that the above invention can be subjected to many changes and variations. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A method for treating piggery wastewater using direct ammonia oxidizing bacteria, characterized in that: include: Resuscitation and activation of direct ammonia oxidizing bacteria; Growth culture of direct ammonia oxidizing bacteria; Direct ammonia oxidizing bacteria to treat actual piggery wastewater; The MBBR reactor was started with direct ammonia oxidizing bacteria as inoculum and simulated piggery wastewater as influent.

2. A method for treating piggery wastewater using direct ammonia oxidizing bacteria as claimed in claim 1, characterized in that: The resuscitation and activation of the direct ammonia oxidizing bacteria is specifically as follows: the culture medium is prepared: 0002 nutrient broth agar, and the specific ingredients are as follows: 5.0g of peptone, 3.0g of beef extract, 5.0g of NaCl, 1000ml of distilled water, pH 7.0-7.

5. The above ingredients are sterilized at 121-126°C for 20-30min, and the operation is performed on a clean bench, and the strain CGMCC1.1837 is transferred to the above culture medium for strain resuscitation.

3. A method for treating piggery wastewater using direct ammonia oxidizing bacteria as claimed in claim 1, characterized in that: The culture conditions for the growth and cultivation of the direct ammonia oxidizing bacteria are: ammonia nitrogen concentration of 500-600 mg / L, C / N of 8-10, salinity of 0.2%-0.3%, pH of 7.0-7.5, carbon source of CH3COONa, and temperature of 29.5-30.5°C.

4. A method for treating piggery wastewater using direct ammonia oxidizing bacteria as claimed in claim 1, characterized in that: The specific steps of direct ammonia oxidizing bacteria treatment of actual pig farm wastewater are as follows: the obtained pig farm wastewater is filtered, the filtered pig farm wastewater is sterilized at 121-126°C for 20-30 minutes, the above-mentioned direct ammonia oxidizing strain CGMCC1.1837 is inoculated on the clean bench, and sealed with a sealing film.

5. A method for treating piggery wastewater using direct ammonia oxidizing bacteria as claimed in claim 1, characterized in that: The inoculated piggery wastewater was placed in a constant temperature oscillator; The constant temperature oscillation conditions are a culture temperature of 29.5-30.5°C, a rotation speed of 150-160 rpm, and a cycle of 7-9 days.

6. A method for treating piggery wastewater using direct ammonia oxidizing bacteria as claimed in claim 1, characterized in that MB The influent of the BR reactor was simulated pig farm wastewater: (NH4)2SO4 was used as the nitrogen source, CH3COONa as the carbon source, K2HPO4 as the phosphorus source, and the direct ammonia oxidizing bacteria CGMCC1.1837 as the inoculum.

7. Use of the method for treating piggery wastewater using direct ammonia oxidizing bacteria as described in any one of claims 1 to 6 in treating piggery wastewater.

Images