Formula camel milk powder with function of reducing blood pressure

Abstract

The invention relates to the technical field of modified milk powder preparation, and discloses a formula camel milk powder with a blood pressure lowering function, which comprises the following raw materials: camel milk powder, a vitamin composition and an auxiliary blood pressure lowering composition, the vitamin composition comprises riboflavin and D-alpha-tocopherol; the composition for assisting in lowering blood pressure comprises an astragalus sinicus flower extract and an achyranthes bidentata extract; a brand-new formula is found through a large number of experiments, the vitamin composition, the auxiliary blood pressure reducing composition and the camel milk powder can generate a synergistic effect, the blood pressure reducing function of the camel milk powder is remarkably improved, the time for taking the blood pressure reducing function of the camel milk powder is shortened, and the blood pressure reducing taking dosage of the camel milk powder is reduced. In addition, the selected raw materials are all from nutrients necessary for human bodies and extracts of medicinal and edible materials, so that the health-care food has good safety and wide application prospects.

Description

Technical Field

[0001] The invention relates to the technical field of preparation of modulated milk powder, in particular to a formula camel milk powder with blood pressure lowering function. Background Art

[0002] Camel milk has high nutritional value and is rich in vitamin C, amino acids and unsaturated fatty acids (such as oleic acid, linoleic acid, etc.). These ingredients have certain benefits for cardiovascular health. For example, unsaturated fatty acids help lower cholesterol levels, thereby indirectly assisting in controlling blood pressure. However, current nutritional and pharmacological studies have not found that camel milk has the effect of directly lowering blood pressure, and the treatment of hypertension still needs to rely on standardized drug treatment and lifestyle adjustments. In recent years, medicinal and edible materials have been increasingly valued by food researchers because of their high safety and good effects. Among them, ginger, wolfberry, and gardenia are common medicinal and edible materials with multiple biological activities such as antioxidant and blood pressure lowering, and have great application potential in functional foods. However, there are currently few studies on camel milk and medicinal and edible materials, and it is not known what role camel milk and medicinal and edible materials can play in the field of cardiovascular health.

[0003] Patent CN202110639517.5 discloses a method for preparing high-protein camel milk powder, including the following steps: pretreatment, irradiation, mixing, hydrolysis and spray drying. Camel milk powder includes fresh camel milk, yam powder and maltodextrin. The patent states that the high-protein camel milk powder prepared has good ACE (angiotensin converting enzyme) inhibitory activity and DPPH free radical scavenging rate, and therefore has good blood pressure lowering and antioxidant effects. However, its main raw materials are only fresh camel milk and yam powder, and it is difficult to understand the sources of high protein, blood pressure lowering and antioxidant effects, and it is impossible to predict how much blood pressure lowering effect it can have in actual use.

[0004] Therefore, there is an urgent need to combine camel milk and edible materials to prepare a new formula camel milk powder with blood pressure lowering function, so that the beneficial ingredients in camel milk and edible materials can work synergistically, expand the application of camel milk in functional foods, and improve its blood pressure lowering function. Summary of the invention

[0005] In order to solve the above-mentioned existing problems, the present invention discloses a formula camel milk powder with blood pressure lowering function, and a new formula is found. The vitamin composition, the auxiliary blood pressure lowering composition and the camel milk powder can produce a synergistic effect, significantly improve the blood pressure lowering function of the camel milk powder, shorten the time for the blood pressure lowering function of the camel milk powder to take effect, and reduce the blood pressure lowering dosage of the camel milk powder. In addition, the raw materials selected by the present invention are all derived from essential nutrients for the human body and extracts of medicinal and edible materials, have good safety, and have broad application prospects.

[0006] The blood pressure lowering function of the camel milk formula of the present invention has the following implications: first, after taking the camel milk formula for a period of time, the blood pressure range of individuals with hypertension is reduced as a whole, and the symptoms of hypertension are alleviated; second, compared with taking camel milk or some components in the formula alone, the blood pressure range of individuals with hypertension who take the camel milk formula is lower as a whole; third, after taking the camel milk formula for a period of time, the risk of developing hypertension in individuals who do not suffer from hypertension is reduced; fourth, compared with taking camel milk or some components in the formula alone, the risk of developing hypertension in individuals who do not suffer from hypertension who take the camel milk formula is lower.

[0007] On the one hand, the present invention provides a formula camel milk powder with blood pressure lowering function, comprising the following raw materials: camel milk powder, a vitamin composition and an auxiliary blood pressure lowering composition; the vitamin composition comprises riboflavin and D-α-tocopherol; the auxiliary blood pressure lowering composition comprises astragalus flower extract and Achyranthes bidentata extract.

[0008] Camel milk powder is mainly fresh camel milk obtained through modern milk powder manufacturing technology, and auxiliary materials, food additives, etc. can be selectively added; Riboflavin is vitamin B2, a type of B vitamins, and a component of the body's flavonoids. If it is deficient, it can affect the body's biological oxidation and cause metabolic disorders. The lesions are mostly manifested as inflammation of the mouth, eyes and external genitalia, such as angular cheilitis, cheilitis, glossitis, conjunctivitis and scrotitis, etc. Therefore, this product is often used to prevent and treat the above diseases. The body's storage of vitamin B2 is very limited and needs to be supplemented by diet every day; D-α-tocopherol is a type of vitamin E and is used in medical, health care, feed, food and cosmetics industries. It has a wide range of uses. For example, its deficiency can cause muscle atrophy, infertility and miscarriage. It is clinically used for the treatment of habitual abortion, threatened abortion, infertility and menopausal disorders, progressive muscular dystrophy, coronary heart disease, hyperlipidemia, atherosclerosis, etc.; Milk vetch flower extract and Chinese cyperus rotundus extract are substances extracted from the flowers of the herb Milk vetch and the Chinese medicinal material Chinese cyperus rotundus slices, respectively, and are rich in flavonoids, saponins and other plant active ingredients. Milk vetch has the effects and functions of clearing away heat and detoxifying, diuresis and swelling, cooling blood and stopping bleeding, clearing the liver and improving eyesight, as well as enhancing immunity. Chinese cyperus rotundus has the effects of nourishing the liver and kidney, strengthening the tendons and bones, and is mainly used to treat liver and kidney deficiency, soreness of the waist and knees, or long-term rheumatism and pain due to liver and kidney deficiency.

[0009] The present invention finds that there is a synergistic effect between camel milk powder, a vitamin composition and an auxiliary blood pressure lowering composition. After being prepared into a formula camel milk powder and taken with water, it can promote the decrease of blood pressure in hypertensive individuals. Long-term use can reduce the blood pressure range and relieve the symptoms of hypertension. The overall synergistic effect is caused by the synergistic effect between the active ingredients in camel milk, the vitamin composition, the Chinese milk vetch flower extract and the Chinese rhubarb extract. The synergistic effect is manifested as follows: when any one of the raw materials is missing or the content of any one of the raw materials is lower than the minimum value determined by the experiment, the blood pressure lowering effect of the formula camel milk powder is significantly reduced. Obviously, this is caused by the lack of interaction between the active ingredients of the raw materials or the lack of physiological pathways related to the corresponding active ingredients. Therefore, one of the reasons for the synergistic effect is the interaction between the active ingredients, which is easy to understand. There are complex synergistic and antagonistic mechanisms between plant flavonoid saponins and between them and other active ingredients. The second reason for the synergistic effect may be that multiple active ingredients participate in and synergistically promote the same physiological pathway related to lowering blood pressure. The third reason for the synergistic effect may be that multiple active ingredients participate in multiple physiological pathways related to lowering blood pressure, and multiple physiological pathways are activated simultaneously to significantly produce a blood pressure lowering effect.

[0010] In some embodiments, the preparation method of the astragalus flower extract is: drying and crushing the astragalus flowers to obtain astragalus flower powder, using a polar solvent to dissolve the astragalus flower powder, filtering, distilling, and drying.

[0011] At present, there have been many studies on the preparation methods of astragalus flower extracts, mainly using polar solvents to extract the active ingredients in astragalus flowers and eliminating irrelevant and toxic components. It is understandable that the astragalus flower extracts obtained by different extraction methods have similar effects in the formula camel milk powder of the present invention, because even if the extraction methods are different, the main component astragaloside is extracted; and it is also understandable that the effects of astragalus flower extracts obtained by different extraction methods in the formula camel milk powder of the present invention may also be significantly different, because the types of active ingredients contained and the ratios between different active ingredients are different, which may be critical to the complex synergistic and antagonistic mechanisms of flavonoid saponins.

[0012] Furthermore, the polar solvent used to dissolve the astragalus powder includes a choline chloride-glycerol low eutectic solvent.

[0013] A low eutectic solvent is a two-component or three-component low eutectic mixture composed of a certain stoichiometric ratio of hydrogen bond acceptors (such as quaternary ammonium salts) and hydrogen bond donors (such as compounds such as amides, carboxylic acids and polyols). Its freezing point is significantly lower than the melting point of each component pure substance. The physicochemical properties of low eutectic solvents are very similar to those of ionic liquids, so some people classify it as a new type of ionic liquid or ionic liquid analog. Choline chloride-glycerol low eutectic solvent is a two-component low eutectic mixture composed of choline chloride as a hydrogen bond acceptor and glycerol as a hydrogen bond donor.

[0014] At present, various studies have pointed out that the extraction efficiency and extraction selectivity of active ingredients of deep eutectic solvents prepared by different hydrogen bond acceptors and hydrogen bond donors are different. After a large number of experiments, the present invention found that the blood pressure lowering effect of the formula camel milk powder prepared by using the final obtained milk vetch flower extract by using choline chloride-glycerol deep eutectic solvent to extract milk vetch flower was significantly improved, which is related to the difference in active ingredients of the extracted milk vetch flower extract.

[0015] In some embodiments, the preparation method of the Achyranthes bidentata extract is: taking Achyranthes bidentata slices of the Chinese medicinal material, crushing them, boiling and extracting them, subjecting the extract to graded precipitation with ammonium sulfate, desalting the precipitation by dialysis, and freeze-drying.

[0016] Preferably, in the auxiliary blood pressure lowering composition, the mass ratio of the Astragalus membranaceus flower extract to the Achyranthes bidentata extract is 1:12-25.

[0017] The ratio of Astragalus membranaceus flower extract and Achyranthes bidentata extract has a certain influence on the complex synergistic and antagonistic mechanisms between plant flavonoid saponins and between them and other active ingredients. According to the experiments of the present invention, the content of Astragalus membranaceus flower extract should be low, but should be higher than the minimum value. Within this range, the synergistic effect is more obvious and the blood pressure lowering effect is higher.

[0018] In some embodiments, in the vitamin composition, the mass ratio of riboflavin to D-α-tocopherol is 5-10:1.

[0019] In some embodiments, the mass ratio of the camel milk powder, the vitamin composition and the auxiliary blood pressure lowering composition is 70-90:2-5:10-20.

[0020] Furthermore, the auxiliary blood pressure lowering composition also includes white kidney bean extract and polydextrose.

[0021] White kidney bean extract is a natural amylase inhibitor extracted from white kidney bean. Its main active ingredient is α-amylase inhibitory protein, which exists in the endosperm of white kidney bean seeds. Its specific glycoprotein structure can bind to the glycoside cleavage site on the α-amylase molecule and cause the conformation of the α-amylase molecule to change, causing it to lose the ability to bind to starch glycosides and thus lead to loss of catalytic activity. It is a glycoprotein with N-terminal glycosylation. White kidney bean extract is often used in the production of weight loss functional foods and the research and development of diabetes drugs in the food and pharmaceutical fields. Polydextrose is a water-soluble dietary fiber. It is a D-glucose polymer made from glucose, sorbitol and citric acid. The mixture is mixed in a specific proportion and heated into a molten state, and then subjected to vacuum condensation. Polydextrose is a random condensation product of D-glucose, mainly bonded by 1,6-glycosidic bonds, with an average molecular weight of about 3200, a limiting molecular weight of less than 22000, and an average degree of polymerization of 20. It is usually used as a food component for health functions. Polydextrose can supplement the water-soluble dietary fiber needed by the human body. After entering the human digestive system, it produces special physiological metabolic functions, thereby preventing and treating constipation and fat deposition.

[0022] When studying the effect of long-term use of formula camel milk powder on the overall range of individual blood pressure, the present invention accidentally discovered that the blood pressure lowering effect time of one of the formulas was significantly shortened. After further conducting a large number of experiments, the above formula was obtained, that is, the auxiliary blood pressure lowering composition also includes white kidney bean extract and polydextrose. The white kidney bean extract can regulate the digestion process of polydextrose in the human digestive system. These two ingredients may play a role in assisting the effect of Astragalus membranaceus flower extract and Achyranthes bidentata extract, or changing the composition structure of the active ingredients in the previous formula, thereby accelerating the onset time of the formula camel milk powder for long-term control of blood pressure.

[0023] On the other hand, the present invention provides a use of an astragalus flower extract for preparing an agent for improving the blood pressure lowering effect of formula camel milk powder. The preparation method of the astragalus flower extract comprises extracting the astragalus flower using a choline chloride-glycerol low eutectic solvent; the formula camel milk powder comprises camel milk powder, a vitamin composition and an auxiliary blood pressure lowering composition, the vitamin composition comprises riboflavin and D-α-tocopherol, and the auxiliary blood pressure lowering composition comprises the astragalus flower extract and the Achyranthes bidentata extract.

[0024] On the other hand, the present invention provides a composition for preparing an agent for shortening the blood pressure lowering onset time of camel milk formula powder, the composition comprising white kidney bean extract and polydextrose, the camel milk formula powder comprising camel milk powder, a vitamin composition and an auxiliary blood pressure lowering composition, the vitamin composition comprising riboflavin and D-α-tocopherol, and the auxiliary blood pressure lowering composition comprising astragalus flower extract and Achyranthes bidentata extract.

[0025] In summary, the present invention includes at least one of the following beneficial technical effects:

[0026] 1. The present invention discloses a camel milk powder formula with a blood pressure lowering function. A new formula has been found. The auxiliary blood pressure lowering composition and the camel milk powder can produce a synergistic effect, significantly improve the blood pressure lowering function of the camel milk powder, shorten the time for the blood pressure lowering function of the camel milk powder to take effect, and reduce the blood pressure lowering dosage of the camel milk powder. In addition, the raw materials selected by the present invention are all derived from essential nutrients for the human body and extracts of medicinal and edible materials, which have good safety and broad application prospects.

[0027] 2. The present invention found that the preparation of astragalus flower extract using different methods has an impact on the blood pressure lowering function of formula camel milk powder. Among them, the present invention found that the astragalus flower was extracted using choline chloride-glycerol low eutectic solvent, and the blood pressure lowering effect of the formula camel milk powder prepared using the astragalus flower extract obtained was significantly improved, which is related to the difference in active ingredients of the extracted astragalus flower extract;

[0028] 3. The present invention accidentally discovered that white kidney bean extract and polydextrose can shorten the onset time of long-term blood pressure lowering of formula camel milk powder. These two ingredients may have played a role in assisting the effect of Astragalus membranaceus flower extract and Achyranthes bidentata extract, or changing the composition structure of the active ingredients in the previous formula, thereby accelerating the onset time of long-term blood pressure control of formula camel milk powder. DETAILED DESCRIPTION

[0029] The present invention is further described in detail below in conjunction with specific examples, which are only used to explain the present invention and are not used to limit the scope of the present invention. The materials, reagents, etc. used in the following examples are reagents and materials available from commercial sources unless otherwise specified.

[0030] Example 1: Preparation of camel milk powder with blood pressure lowering function

[0031] First, fresh camel milk transported from the pasture is taken and filtered using a double filter to remove most of the impurities and microorganisms in the fresh camel milk through filtration and adsorption; then it is purified using a milk purifier to further remove mammary gland cells and certain microorganisms in the fresh camel milk; then it is sent to a spray drying equipment for spray drying to obtain camel milk powder.

[0032] Commercially available riboflavin and D-α-tocopherol were mixed in a mass ratio of 8:1 to obtain a vitamin composition.

[0033] Preparation of astragalus flower extract: Take freshly picked astragalus flowers, place them in a 40°C oven for low-temperature drying, take out the dried flowers for crushing, add choline chloride-glycerol low eutectic solvent to the astragalus flower powder, and refer to the existing public method for the preparation of choline chloride-glycerol low eutectic solvent. Stir choline chloride and glycerol at a mass ratio of 1:5 at 60°C and cool them down. Extract astragalus flower powder with choline chloride-glycerol low eutectic solvent at room temperature for 3h, filter, distill under reduced pressure, and dry to obtain a solid, which is astragalus flower extract, the main component of which is astragalus glycosides, and it also contains a variety of trace plant active ingredients. The preparation of the extract of Chinese herbal medicine is based on the existing public method, and the Chinese herbal medicine slices of Chinese herbal medicine are taken, crushed, boiled and extracted, and the extract is graded and precipitated with ammonium sulfate, and the precipitation is dialyzed for desalination and freeze-dried. The astragalus flower extract and the Chinese herbal medicine extract are mixed at a mass ratio of 1:15 to obtain an auxiliary blood pressure lowering composition.

[0034] The camel milk powder, vitamin composition and auxiliary blood pressure lowering composition obtained in the above steps are placed in a fluidized bed, mixed evenly on the fluidized bed according to a mass ratio of 80:5:15, and sieved to obtain a formula camel milk powder with blood sugar lowering function.

[0035] ACE inhibitory activity is correlated with the blood pressure lowering effect of the product. It is generally believed that an ACE inhibition rate of more than 50% has a significant blood pressure lowering effect. ACE inhibitory activity detection is mainly an in vitro test. By adding a simulated substrate of angiotensin I, FAPGG (fluorescein acetylprocainamide-proline-glycine), it acts on ACE under certain conditions, and the absorbance of FAPGG at 340nm is measured by an ELISA instrument to calculate the activity of ACE. The ACE inhibition rate kit of abcam is used for detection. The detection principle is the same as above. The detection method refers to the kit instructions. The above-mentioned camel milk powder is configured into a 0.2g / mL milk powder solution for detection, and the ACE inhibition rate is 71.46%, showing that the formula camel milk powder of this embodiment has an effective blood pressure lowering effect.

[0036] Example 2: Experiment on the blood pressure lowering effect of camel milk powder with different formulas

[0037] This example selected a portion of camel milk formula powders that were significant in previous experiments and the test results of their ACE inhibition rates to study the generation of the synergistic effect of the formula on lowering blood pressure and its causes.

[0038] First, the camel milk powder formula prepared by the method of Example 1 is taken, recorded as Formula 1, and then the following camel milk powder formula is prepared by referring to the method of Example 1:

[0039] Formula 2: 90 parts of camel milk powder, 10 parts of auxiliary blood pressure lowering composition;

[0040] Formula 3: 90 parts of camel milk powder, 10 parts of vitamin composition;

[0041] Formula 4: 80 parts of camel milk powder, 5 parts of riboflavin, and 15 parts of the auxiliary blood pressure lowering composition;

[0042] Formula 5: 80 parts of camel milk powder, 5 parts of vitamin composition (riboflavin: D-α-tocopherol = 12:1), 15 parts of auxiliary blood pressure lowering composition;

[0043] Formula 6: 80 parts of camel milk powder, 5 parts of vitamin composition, 15 parts of Achyranthes bidentata extract;

[0044] Formula 7: 80 parts of camel milk powder, 5 parts of vitamin composition, 15 parts of auxiliary blood pressure lowering composition (Astragalus membranaceus flower extract: Cyathula officinalis extract = 1:30);

[0045] The above-mentioned camel milk powder formulas were respectively tested for ACE inhibition rate according to the method described in Example 1, and each formula was tested in parallel 3 times to calculate the average value. The results are shown in Table 1.

[0046] Table 1: ACE inhibition rate of camel milk powder with different formulas (n=3)

[0047]

[0048]

[0049] According to the experimental results, formulas 2-7 do not have effective blood pressure lowering effects. Combined with formulas 1-3, it can be seen that only when camel milk powder, vitamin composition, and auxiliary blood pressure lowering composition are present at the same time, the formula camel milk powder can have the effect of lowering blood pressure, indicating that there is a synergistic effect between the above three; and as for which specific substances have a synergistic effect, the results of formulas 4 and 6 show that riboflavin and D-α-tocopherol, astragalus flower extract and Achyranthes bidentata extract all need to be present in the formula to achieve a synergistic effect. Combined with the experimental results of formulas 5 and 7, it can be seen that even if the content of D-α-tocopherol and astragalus flower extract is extremely low, it can also significantly improve the ACE inhibition rate, suggesting that the active ingredients of D-α-tocopherol and astragalus flower extract may play a catalytic or complex coordination mechanism, which is also the source of the synergistic effect.

[0050] In order to clarify the composition ratio of each ingredient, it was observed that the ACE inhibition rate of formula 5 and formula 7 was only slightly greater than 50%, and the content of D-α-tocopherol and astragalus flower extract was directly determined as the minimum value; further proportion experiments showed that the mass ratio of astragalus flower extract and Achyranthes bidentata extract was preferably 1:12-25, and the ACE inhibition rate was >55%, and the mass ratio of riboflavin and D-α-tocopherol was preferably 5-10:1, and the ACE inhibition rate was >55%; in addition, the mass ratio of camel milk powder, vitamin composition and auxiliary blood pressure lowering composition was preferably 70-90:2-5:10-20, and the ACE inhibition rate was >55%.

[0051] Example 3: Effects of different preparation methods of Chinese Milkvetch flower extract on blood pressure lowering efficacy

[0052] Generally speaking, the preparation method of astragalus flower extract is to extract astragalus dried flowers using polar organic solvents. Since the main active ingredient in astragalus flower is astragaloside, it is generally believed that ethyl acetate or acetone should be used for extraction. The present invention also selected ethyl acetate for extraction in previous studies, but it was found that the ACE inhibition rate of the final formula camel milk powder prepared was low; it was also found that the preparation methods of astragalus flower extracts were slightly different in multiple experiments, but the final ACE inhibition rates were quite different. Therefore, it is speculated that the trace amounts of certain components in the astragalus flower extract have a greater influence on the final blood pressure lowering effect, and different preparation methods have an influence on the extraction selectivity of the components.

[0053] This example selects several methods for preparing astragalus flower extracts in previous experiments, and the ACE inhibition rate test results of formula camel milk powder prepared using astragalus flower extracts prepared according to these methods. The specific method for preparing astragalus flower extracts is as follows:

[0054] Method 1 (Example 1): Take freshly picked astragalus flowers, place them in an oven at 40°C for low-temperature drying, take out the dried flowers and crush them, add choline chloride-glycerol low eutectic solvent to the astragalus flower powder, the preparation of choline chloride-glycerol low eutectic solvent refers to the existing public method, choline chloride and glycerol are stirred at room temperature in a mass ratio of 1:5, and the astragalus flower powder is extracted with choline chloride-glycerol low eutectic solvent at room temperature for 3h, filtered, distilled under reduced pressure, and dried to obtain a solid;

[0055] Method 2: The only difference from method 1 is that the choline chloride-glycerol deep eutectic solvent is replaced by ethyl acetate;

[0056] Method 3: The only difference from method 1 is that the choline chloride-glycerol deep eutectic solvent is replaced by n-butanol;

[0057] Method 4: The only difference from method 1 is that the choline chloride-glycerol deep eutectic solvent is replaced by a betaine-glycerol deep eutectic solvent, and the addition ratio of betaine is the same as that of choline chloride;

[0058] Method 5: The only difference from method 1 is that the choline chloride-glycerol deep eutectic solvent is replaced by the choline chloride-phenylacetic acid deep eutectic solvent, and the addition ratio of phenylacetic acid is the same as that of glycerol;

[0059] Method 6: The only difference from Method 1 is that choline chloride and glycerol are stirred evenly at room temperature in a mass ratio of 1:2.

[0060] The ACE inhibition rate test results of the camel milk powder formula prepared with the Chinese astragalus flower extract prepared by the above 6 methods are shown in Table 2. Each group was tested in parallel 3 times to calculate the average value.

[0061] Table 2: ACE inhibition rate of different preparation methods of Chinese milk vetch flower extract (n=3)

[0062] ACE inhibition rate Method 1 71.46％ Method 2 51.69％ Method 3 52.47％ Method 4 55.40％ Method 5 57.01％ Method 6 70.96％

[0063] According to the experimental results, when using solvents other than choline chloride-glycerol low eutectic solvent for extraction, the ACE inhibition rate of formula camel milk powder is low, only slightly higher than 50%. When using solvents other than choline chloride-glycerol low eutectic solvent for extraction, the ACE inhibition rate of formula camel milk powder can reach more than 70%, which has a significant effect on lowering blood pressure. The reason is that different solvents have different extraction selectivity for plant active ingredients, and different combinations and proportions of plant active ingredients have a significant impact on the synergistic effect between them and other active ingredients in formula camel milk powder.

[0064] Example 4: Animal blood pressure lowering test of camel milk formula

[0065] In the above embodiment, the formula camel milk powder of the present invention shows a strong ACE inhibition rate. In this embodiment, an animal experiment is conducted to verify that the formula camel milk powder of the present invention has a good blood pressure lowering effect on individuals; the experimental animals are 6-week-old SPF-grade SD male rats, weighing 260±10g, and the feeding conditions are 12h light / dark alternation, temperature 22±1℃, relative humidity 50-60%, free access to water and diet, and after acclimating to the environment for 1 week, rats with no significant change in weight are selected to start the experiment. First, the rats are randomly divided into 2 groups, wherein the control group rats are fed with ordinary feed, and the model group rats are induced with high-salt feed to induce hypertension, and the high-salt feed is an ordinary feed with 8wt% NaCl added; the tail artery blood pressure of the rats is measured weekly during the feeding period. After 2 weeks of feeding, the tail artery blood pressure of the control group rats has no significant change from the beginning of the experiment, and the next step of the experiment is entered. After 2 weeks of feeding, the tail artery blood pressure of the model group rats has a significant increase from the beginning of the experiment, and the next step of the experiment is entered.

[0066] The rats entering the next step of the experiment were grouped, with 20 rats in each group; the control group rats were divided into a blank control group, and the feeding mode during the experiment did not change. The model group rats were further divided into a negative control group, a positive control group and an experimental group. The negative control group was gavaged with 5 mg / kg of captopril (Bayer) daily, which was configured into a solution for gavage; the feeding mode of the positive control group was not changed, and the experimental group was gavaged with 2 mL / 100 g of formula camel milk powder (Example 1) solution daily, and the concentration of the formula camel milk powder solution was 0.2 g / mL; the experiment was carried out for a total of 4 weeks, during which the tail artery blood pressure of the test rats was measured every week, and the mean of systolic and diastolic blood pressure was calculated in the group, respectively. The results are shown in Table 3.

[0067] Table 3: Rat blood pressure reduction experiment (n=20)

[0068]

[0069]

[0070] According to the above experimental results, the blank control showed the blood pressure of normal rats, the positive control showed the blood pressure of hypertension model rats, and the rats in the negative control group reduced their systolic blood pressure by about 13 mmHg and diastolic blood pressure by about 8 mmHg by taking antihypertensive drugs. However, under the feeding conditions of inducing hypertension, the blood pressure could not be further reduced. The rats in the experimental group were gavaged with the formula camel milk powder of the present invention. Although the blood pressure reduction in the first two weeks was not as large as that of drug intervention, with continued gavage, the antihypertensive effect of the formula camel milk powder of the present invention was improved, and the blood pressure of the rats in the experimental group was much lower than that of the negative control group.

[0071] Example 5: Effects of different auxiliary blood pressure lowering compositions on long-term blood pressure lowering efficacy

[0072] In the above examples, the camel milk formula of the present invention shows a strong blood pressure lowering effect, that is, the camel milk formula of the present invention can promptly lower the individual's blood pressure after taking and digesting and absorbing, but the overall control of the individual's blood pressure range after long-term use is not discussed. This example attempts to use different auxiliary blood pressure lowering compositions to prepare camel milk formula, hoping to find a formula that can control the individual's blood pressure range after taking it for a period of time.

[0073] This example introduces an animal experiment. The experimental animals are 6-week-old SPF SD male rats, weighing 260±10g. The feeding conditions are 12h light / dark alternation, temperature 22±1℃, relative humidity 50-60%, free access to water and diet, and after acclimating to the environment for 1 week, rats with no significant change in weight are selected to start the experiment. First, the rats are randomly divided into 2 groups, where the control group rats are fed with ordinary feed, and the model group rats are induced to have high blood pressure with high-salt feed, which is ordinary feed with additional 8wt% NaCl; the tail artery blood pressure of the rats is measured weekly during the feeding period. After 2 weeks of feeding, the tail artery blood pressure of the control group rats has no significant change from the beginning of the experiment, and the next step of the experiment is entered. After 2 weeks of feeding, the tail artery blood pressure of the model group rats has a significant increase from the beginning of the experiment, and the next step of the experiment is entered.

[0074] Long-term blood pressure lowering experiment: It is mainly to verify that after taking camel milk powder for a period of time, the blood pressure of hypertensive individuals can still be controlled during the period of stopping. 10 rats in the above model group were gavaged with 2mL / 100g camel milk powder solution every day. The concentration of camel milk powder solution was 0.2g / mL. After gavage for 4 weeks, gavage was stopped for 1 week. During this period, the tail artery blood pressure of the test rats was measured every week.

[0075] Long-term use to prevent hypertension experiment: mainly to verify that after healthy individuals stop taking camel milk powder for a period of time and replace it with a diet that induces hypertension, the blood pressure can still be controlled. Take 10 rats in the above control group and gavage 2mL / 100g camel milk powder solution daily. The concentration of camel milk powder solution is 0.2g / mL. After 4 weeks of gavage, stop gavage for 1 week and replace the ordinary feed with high-salt feed. During this period, the tail artery blood pressure of the test rats is measured every week.

[0076] The formula camel milk powder prepared in Example 1 was introduced into the above two experiments, and it was found that the tail artery blood pressure of the rats in the model group did not increase significantly one week after stopping gavage (systolic blood pressure mean 101.2 mmHg, diastolic blood pressure mean 86.8 mmHg) compared with before stopping gavage (systolic blood pressure mean 102.7 mmHg, diastolic blood pressure mean 85.2 mmHg), and the tail artery blood pressure of the rats in the control group after replacing the high-salt feed for one week (systolic blood pressure mean 94.4 mmHg, diastolic blood pressure mean 72.1 mmHg) was not significantly increased compared with before stopping gavage (systolic blood pressure mean 94.1 mmHg, diastolic blood pressure mean 71.4 mmHg), indicating that the formula camel milk powder of the present invention has a certain effect of long-term control of blood pressure and prevention of hypertension.

[0077] In order to further clarify the time required for long-term camel milk powder to be taken for long-term control of blood pressure and its relationship with the formula, the present invention conducted animal tests on camel milk powder of various formulas selected and prepared in the previous experiments, and found that one of the formulas of camel milk powder can control the blood pressure of model group mice for 1 week after gavage for 2 weeks. In this formula, based on the formula of Example 1, the auxiliary blood pressure lowering composition also contains white kidney bean extract and polydextrose, and the specific mass ratio is astragalus flower extract: Achyranthes bidentata extract: white kidney bean extract: polydextrose = 1:15:1:5. The principle of shortening the onset time of long-term blood pressure control may be that the white kidney bean extract regulates the individual's digestion process of polydextrose, and polydextrose and its metabolites can be combined with other active ingredients to promote individual absorption and utilization, as well as affect the pattern of bioavailability and related physiological pathways.

[0078] In order to further study the correlation between the relevant active ingredients and blood pressure control, this example tested coix seed, cassia seed, etc., which have similar effects to white kidney bean, and oligofructose (FOS), lactulose, etc., which have similar effects to polydextrose. It was found that when the above formula was used, the overall blood pressure lowering effect was even worse than the camel milk powder formula in Example 1, indicating that there may be antagonism between the above formula and the original formula. Only the formula containing white kidney bean extract and polydextrose can shorten the onset time of long-term blood pressure control.

[0079] Further ratio tests showed that the white kidney bean extract and polydextrose can be effective when the content of white kidney bean extract is lower, and the preferred ratio is 1:3-15; the best ratio of white kidney bean extract to astragalus flower extract is 1:1-5.

[0080] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto, and any equivalent modifications or substitutions that can be easily thought of by any technician familiar with the technical field within the technical scope disclosed by the present invention should be included in the protection scope of the present invention.

Claims

1. A camel milk powder formula with blood pressure lowering function, characterized in that: The invention comprises the following raw materials: camel milk powder, a vitamin composition and an auxiliary blood pressure lowering composition; the vitamin composition comprises riboflavin and D-α-tocopherol; the auxiliary blood pressure lowering composition comprises astragalus flower extract and cyathula officinalis extract.

2. The camel milk powder formula with blood pressure lowering function as claimed in claim 1, characterized in that: The preparation method of the astragalus flower extract comprises the following steps: drying and crushing the astragalus flowers to obtain astragalus flower powder, dissolving the astragalus flower powder with a polar solvent, filtering, distilling and drying.

3. The camel milk powder formula with blood pressure lowering function as claimed in claim 2, characterized in that: The polar solvent used to dissolve astragalus powder includes choline chloride-glycerol deep eutectic solvent.

4. The camel milk powder formula with blood pressure lowering function according to claim 1, characterized in that: The preparation method of the Achyranthes bidentata extract comprises the following steps: taking Achyranthes bidentata slices of Chinese medicinal material, grinding them, boiling and extracting them, subjecting the extract to graded precipitation with ammonium sulfate, performing precipitation and dialysis to desalt, and freeze-drying.

5. The camel milk powder formula with blood pressure lowering function according to claim 1, characterized in that: In the auxiliary blood pressure lowering composition, the mass ratio of the Chinese milk vetch flower extract to the Chinese rhizome of radix achyranthis Bidentatae extract is 1:12-25.

6. The camel milk powder formula with blood pressure lowering function according to claim 1, characterized in that: In the vitamin composition, the mass ratio of riboflavin to D-α-tocopherol is 5-10:

1.

7. The camel milk powder formula with blood pressure lowering function according to claim 1, characterized in that: The mass ratio of the camel milk powder, the vitamin composition and the auxiliary blood pressure lowering composition is 70-90:2-5:10-20.

8. The camel milk powder formula with blood pressure lowering function according to claim 1, characterized in that: The auxiliary blood pressure lowering composition also includes white kidney bean extract and polydextrose.

9. A use of a Chinese milk vetch flower extract for preparing a reagent for improving the blood pressure lowering effect of formula camel milk powder, characterized in that: The preparation method of the astragalus flower extract comprises extracting astragalus flower using a choline chloride-glycerol low eutectic solvent; the formula camel milk powder comprises camel milk powder, a vitamin composition and an auxiliary blood pressure lowering composition, the vitamin composition comprises riboflavin and D-α-tocopherol, and the auxiliary blood pressure lowering composition comprises the astragalus flower extract and the Achyranthes bidentata extract.

10. A composition for preparing an agent for shortening the blood pressure lowering effect time of camel milk formula, characterized in that: The composition comprises white kidney bean extract and polydextrose, the formula camel milk powder comprises camel milk powder, a vitamin composition and an auxiliary blood pressure lowering composition, the vitamin composition comprises riboflavin and D-α-tocopherol, and the auxiliary blood pressure lowering composition comprises astragalus flower extract and rhizoma achyranthis Bidentatae extract.

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