Method for producing biological enzyme protein feed by utilizing pineapple peel pomace through biological enzyme fermentation

Abstract

The invention relates to the technical field of bio-enzyme protein feed production, and particularly discloses a method for producing a bio-enzyme protein feed by using pineapple peel pomace through bio-enzyme fermentation, which comprises the following steps: 1, carrying out test tube culture, shake-flask culture and seeding tank culture on high-protein high-activity saccharomycetes with the mycoprotein content of more than 40% to obtain high-protein high-activity saccharomycetes with the mycoprotein content of more than 40%; inoculating into a fermentation tank according to the inoculum size of 10%, and culturing for 24 hours to obtain bacterial liquid for production; the pineapple peel pomace treated by the method is simple in process, mature industrial complete equipment is selected, the amount of the pineapple peel pomace treated is large, and the produced product is high in nutritional value, high in livestock palatability, free of chemical additive components and suitable for feeding of various livestock, solves the problem of pollution caused by pomace treatment in fruit factories and fruit can factories at present, and has a good market prospect. The method has obvious environmental benefits, economic benefits and social benefits, and is suitable for treating and converting the pineapple peel pomace of various fruit factories and fruit tin factories and treating the pollution of the pineapple peel pomace to the environment.

Description

Technical Field

[0001] The invention belongs to the technical field of producing protein feed, and in particular relates to a method for producing bio-enzyme protein feed by utilizing pineapple peel pomace through bio-enzyme fermentation. Background Art

[0002] In the agricultural and food processing industries, pineapple peel pomace, as a by-product of juice production, is usually regarded as waste. However, pineapple peel pomace is rich in cellulose, hemicellulose, protein and other nutrients and has potential utilization value. Traditional treatment methods, such as landfill or incineration, not only waste resources but also may pollute the environment. Therefore, it is particularly important to find an efficient and environmentally friendly method to utilize pineapple peel pomace.

[0003] At present, some studies have attempted to convert pineapple peel pomace into feed, but most methods have problems such as low fermentation efficiency, poor feed quality, and high production cost. Especially in the process of microbial fermentation, the difficult-to-degrade components in pineapple peel pomace limit the growth and metabolism of microorganisms, resulting in low protein content and nutritional value in the feed. How to effectively improve the fermentation efficiency of pineapple peel pomace and the nutritional value of feed has become a problem that needs to be solved urgently. To this end, a method for producing bio-enzyme protein feed by bio-enzyme fermentation using pineapple peel pomace is proposed. Summary of the invention

[0004] The purpose of the present invention is to provide a method for producing bio-enzyme protein feed by using pineapple peel pomace through bio-enzyme fermentation, so as to solve the problems of low fermentation efficiency, poor feed quality and high production cost.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A method for producing bio-enzyme protein feed by using pineapple peel pomace through bio-enzyme fermentation, comprising:

[0007] Step 1: The high-protein and high-activity yeast with a bacterial protein content of more than 40% is cultured in a test tube, a shake flask, or a seed tank, and then inoculated into a fermentation tank at a 10% inoculation rate and cultured for 24 hours to obtain a production bacterial liquid;

[0008] Step 2: crushing and mixing the pineapple peel pomace with 1% to 5% of the production bacterial liquid and (the commercially available finished product Ganoderma lucidum polysaccharide, the usage amount is 1% to 5%), and evenly mixing the pineapple peel pomace, loading into a barrel of more than 10 tons, and then sealing and fermenting for 7×24 hours, the fermentation temperature is between 20 and 72°C;

[0009] Step 3: adjusting the pH value of the fermented pineapple peel pomace to below 5.0, and packaging the obtained fermentation product with a breathing bag and storing it in a cool place for later use;

[0010] In the step three, it also includes taking samples from the mixture after the enzymatic hydrolysis reaction to test indicators such as protein content, amino acid composition, enzyme activity, ganoderma polysaccharide, oligosaccharide content, etc., and evaluating the quality of the finished product based on the test results. The evaluation content includes protein conversion rate, amino acid balance and ganoderma polysaccharide activity, oligosaccharide content, etc. to ensure that the quality of the finished product meets the predetermined standards and requirements;

[0011] In step 3, the qualified finished products are packaged, and the sealing, light-proof and moisture-proof properties of the packaging materials should be ensured during the packaging process; after the packaging is completed, the finished products are stored in a dry, cool and well-ventilated storage environment to avoid the influence of high temperature and humidity on the quality of the finished products;

[0012] Step 4: Mix the fermentation product obtained in the above step 3 with corn, fish meal, kelp powder, zeolite powder and oyster shell powder, and extrude the mixture into pellet feed through a forming extruder;

[0013] In step 4, reasonable usage specifications are formulated based on the nutritional requirements and digestive capacity of different poultry and aquatic animals, and the usage should be adjusted according to factors such as the animal's growth stage, weight and breeding environment.

[0014] Preferably, the test tube culture temperature of the high-protein and high-activity yeast is 25-30° C., and the culture time is 48 hours; the shake flask culture temperature is 25-28° C., the rotation speed is 150-250 rpm, and the culture time is 12-24 hours.

[0015] Preferably, the production bacterial liquid contains 10 high-protein and high-activity yeast viable bacteria 6 ~10 9 The pineapple peel pomace is fresh pomace without any processing after peeling in juice factories and canning factories. After the fermentation products are mixed in the step 4, the moisture content thereof should be less than 15%.

[0016] Preferably, in the step one, under the stirring condition of the seed tank, the fermentation temperature is 25-28°C for 12-24 hours, the fermentation tank culture temperature is 25-28°C, the culture time is 24-36 hours, and the seed liquid is obtained, and the seed liquid is inoculated into the fermentation tank at a 10% inoculation amount.

[0017] Preferably, in the step 2, ganoderma lucidum polysaccharide is added to the processed pineapple peel pomace, and the added amount can be adjusted according to the specific formula to enhance the biological activity and nutritional value of the feed.

[0018] Preferably, in step 1, the seed liquid is pretreated with the first biological enzyme, and the first biological enzyme is any one of cellulase or hemicellulase, so as to improve the degradation ability of microorganisms on pineapple peel pomace during the subsequent fermentation process.

[0019] Preferably, in the step 2, the second biological enzyme is used to promote the decomposition of the difficult-to-degrade components in the pineapple peel pomace, and the second biological enzyme is any one of a protease or an amylase, so as to improve the fermentation efficiency and the feed quality.

[0020] Preferably, in the step 4, the mixed fermentation product is extruded through a molding extruder, and pellet feed is produced using molds of different shapes and sizes to meet the needs of different animal feeding;

[0021] In step 4, the quality of the pellet feed produced is tested, including protein content, moisture content and microbial indicators, to ensure that the product meets relevant standards and requirements, and the qualified products are packaged and marked with production date, shelf life and ingredient information for easy storage and sale.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] By introducing the biological enzyme pretreatment technology, the present invention pretreats the pineapple peel pomace before fermentation, thereby reducing the content of the difficult-to-degrade components thereof, improving the degradation ability of the pineapple peel pomace by microorganisms, and at the same time, continuously utilizing the biological enzyme to promote the decomposition of the difficult-to-degrade components during the fermentation process, further improving the fermentation efficiency, thereby enabling the pineapple peel pomace to be more fully converted into high-quality protein feed;

[0024] The present invention adopts high-protein and high-activity yeast for fermentation, and combines the addition of ganoderma lucidum polysaccharide to significantly improve the protein content and nutritional value of the feed. At the same time, by optimizing the fermentation conditions and real-time monitoring and regulation technology, the stability and controllability of the fermentation process are ensured, thereby producing a protein feed with excellent quality and balanced nutrition.

[0025] Compared with the traditional feed production method, the present invention uses waste pineapple peel and pomace as raw materials, which not only reduces the production cost but also realizes the recycling of resources. In addition, through intelligent management and control technology, the production efficiency and product quality are improved, and the production cost is further reduced.

[0026] The implementation of the present invention reduces the amount of landfill and incineration of pineapple peel and pomace, reduces environmental pollution, and at the same time, the produced protein feed can be used for animal breeding, replacing part of traditional feed, reduces dependence on food resources, and helps to achieve sustainable agricultural development.

[0027] In summary, the protein feed produced by the present invention has no chemical additives, has a natural fruit fermentation aroma, has a protein content of about 20%, and contains a variety of amino acids, vitamins and trace elements, has a strong palatability for livestock, and is a high-quality and low-cost nutritious protein feed. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a flow chart of the steps of the present invention. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] A method for producing bio-enzyme protein feed by using pineapple peel pomace through bio-enzyme fermentation, comprising:

[0031] Step 1: The high-protein and high-activity yeast with a bacterial protein content of more than 40% is cultured in a test tube, a shake flask, or a seed tank, and then inoculated into a fermentation tank at a 10% inoculation rate and cultured for 24 hours to obtain a production bacterial liquid;

[0032] Step 2: crushing and mixing the pineapple peel pomace with 1% to 5% of the production bacterial liquid and (the finished product Ganoderma lucidum polysaccharide, the usage amount is 1% to 5%), and evenly mixing the pineapple peel pomace, loading into a barrel of more than 10 tons, and then sealing and fermenting for 7×24 hours, and the fermentation temperature is between 20 and 72°C;

[0033] Step 3: adjusting the pH value of the fermented pineapple peel pomace to below 5.0, and packaging the obtained fermentation product with a breathing bag and storing it in a cool place for later use;

[0034] In the step three, it also includes taking samples from the mixture after the enzymatic hydrolysis reaction to test indicators such as protein content, amino acid composition, enzyme activity, ganoderma polysaccharide, oligosaccharide content, etc., and evaluating the quality of the finished product based on the test results. The evaluation content includes protein conversion rate, amino acid balance and polysaccharide activity to ensure that the quality of the finished product meets the predetermined standards and requirements;

[0035] In step 3, the qualified finished products are packaged, and the sealing, light-proof and moisture-proof properties of the packaging materials should be ensured during the packaging process; after the packaging is completed, the finished products are stored in a dry, cool and well-ventilated storage environment to avoid the influence of high temperature and humidity on the quality of the finished products;

[0036] Step 4: Mix the fermentation product obtained in the above step 3 with corn, fish meal, kelp powder, zeolite powder and oyster shell powder, and extrude the mixture into pellet feed through a forming extruder;

[0037] In step 4, reasonable usage specifications are formulated according to the nutritional requirements and digestive capacity of different poultry and aquatic animals, and the usage should be adjusted according to factors such as the growth stage, weight and feeding environment of the animals;

[0038] Through the setting of the above technical scheme, after poultry and aquatic animals eat the bio-enzyme feed formula, the feed utilization rate is improved, the digestion and absorption of nutrients in the feed are promoted, the anti-nutritional factors in the feed are reduced and eliminated, and the endogenous enzyme deficiency is supplemented, so that the products produced are free of pesticide residues, drug residues, heavy metals, CFU = 2.2x10 to the fifth power or more, and the key is that they are rich in selenium and Ganoderma lucidum polysaccharides and oligosaccharides, etc., and have many trace elements, various acids such as amino acids, glycine, glutamic acid, aspartic acid, methionine, arginine histidine and other ingredients, saturated fatty acids, unsaturated fatty acids, niacin and niacin ammonia = 4.03x10 to the third power or more, and vitamins are also numerous. The more critical function is that it can also lower cholesterol: this is the real purpose of bio-enzyme (removing the inferior and retaining the superior) breeding. Antibiotic replacement and prevention in the breeding process is the highlight of this technical scheme; the direct sun-drying and packaging of poultry manure after breeding is also a growth point of this technical scheme.

[0039] Preparation method 1:

[0040] 20 tons of fresh pineapple peel pomace were fermented and its main components were: water content about 76%; dry matter about 24%; crude protein 6.55; crude ash 6.34; crude fiber 28.8; total sugar 1.16; calcium 2643; phosphorus 1342; pH value below 5.0, viable bacteria count 10 6 ~10 9 / ml high-protein and high-activity yeast liquid, Ganoderma lucidum polysaccharide, etc.

[0041] The high-protein and high-activity yeast strain (commercially available) was inoculated into a 1000 ml shake flask at a temperature of 25-28°C and a speed of 200 rpm. After 18 hours of culture, it was inoculated into a 1 ton seed tank with intermittent stirring at a temperature of 25-28°C. After 20 hours of culture, it was inoculated into a 10 ton fermentation tank with intermittent stirring at a temperature of 25-28°C. After 24 hours of culture, the number of viable bacteria per ml of the fermentation liquid reached 10 6 ~10 9 / ml, which is the mature production bacterial solution;

[0042] 1% to 5% of the bacterial liquid for production and 1% to 5% of the ganoderma lucidum polysaccharide are added to the peeled fresh pineapple peel pomace from a juice factory or a canning factory according to the weight of the pineapple peel pomace, crushed and stirred evenly, and then sealed and fermented at a temperature of 20 to 72° C. for 7×24 hours. The pH value of the pineapple peel pomace is detected to be below 5.0, and the fermentation is completed;

[0043] After fermentation, the water content of the pineapple peel pomace is 70% to 80%. After mixing (corn, fish meal, kelp powder, zeolite powder, oyster shell powder) to reduce the water content to below 15%, the protein feed can be extruded into particles of desired shape and size by an extrusion molding machine, and then weighed and packaged to become a biological enzyme protein feed.

[0044] Preparation method 2:

[0045] 20 tons of fresh pineapple peel pomace were fermented and its main components were: water content about 76%; dry matter about 24%; crude protein 6.55; crude ash 6.34; crude fiber 28.8; total sugar 1.16; calcium 2643; phosphorus 1342; pH value below 5.0, viable bacteria count 10 6 ~10 9 / ml high-protein and high-activity yeast liquid, Ganoderma lucidum polysaccharide, etc.

[0046] The high-protein and high-activity yeast strain (commercially available) was inoculated into a 1000 ml shake flask at a temperature of 25-28°C and a speed of 200 rpm. After 18 hours of culture, it was inoculated into a 1 ton seed tank and intermittently stirred at a temperature of 25-28°C for 20 hours. After that, it was inoculated into a 10 ton fermentation tank with intermittent stirring at a temperature of 25-28°C for 24 hours. The number of viable bacteria per ml of fermentation liquid was 10 6 ~10 9 / ml, which is the mature production bacterial solution;

[0047] 1% to 5% of the bacterial liquid for production and 1% to 5% of the ganoderma lucidum polysaccharide are added to the fresh pineapple peel pomace according to the weight of the pomace, crushed, added with 20% of fish meal, stirred evenly, and then sealed and fermented at a temperature of 20 to 72° C. for 7×24 hours. When the crude protein content of the pineapple pomace is detected to be between 20 and 68 and the pH value is below 5.0, the fermentation is completed;

[0048] As can be seen from the above, by introducing the bio-enzyme pretreatment technology, the present invention pre-treats the pineapple peel pomace before fermentation, thereby reducing the content of the difficult-to-degrade components thereof and improving the degradation ability of the pineapple peel pomace by microorganisms. Meanwhile, during the fermentation process, the bio-enzyme is continuously used to promote the decomposition of the difficult-to-degrade components, thereby further improving the fermentation efficiency. This enables the pineapple peel pomace to be more fully converted into high-quality bio-enzyme protein feed.

[0049] The present invention adopts high-protein and high-activity yeast for fermentation, and combines with the addition of ganoderma lucidum polysaccharide, which significantly improves the protein content and nutritional value of the feed. At the same time, by optimizing the fermentation conditions and real-time monitoring and regulation technology, the stability and controllability of the fermentation process are ensured, thereby producing a biological enzyme protein feed with excellent quality and balanced nutrition.

[0050] Compared with the traditional feed production method, the present invention uses waste pineapple peel and pomace as raw materials, which not only reduces the production cost but also realizes the recycling of resources. In addition, through intelligent management and control technology, the production efficiency and product quality are improved, and the production cost is further reduced.

[0051] The implementation of the present invention reduces the amount of landfill and incineration of pineapple peel and pomace, reduces environmental pollution, and at the same time, the produced bio-enzyme protein feed can be used for animal breeding, replacing part of traditional feed, reducing dependence on food resources, and helping to achieve sustainable agricultural development;

[0052] In summary, the bio-enzyme protein feed produced by the present invention has no chemical additive ingredients, has a natural fruit fermentation aroma, a protein content of more than 20%, and contains a variety of amino acids, vitamins and trace elements, has strong palatability for livestock, and is a nutritious protein feed with high quality and low price. At the same time, the fresh pomace processed by the present invention has a simple process, and the selected equipment is a mature industrial complete set, and a large amount of pomace can be processed. The produced product has high nutritional value, strong palatability for livestock, and has no chemical additive ingredients. It is suitable for feeding various livestock, solves the pollution problem of pomace processing in current juice factories and canning factories, and the enterprises can increase considerable profits, and have significant environmental benefits, economic benefits and social benefits. The present invention is suitable for the processing and transformation of pomace in various juice factories and canning factories, and controls the pollution to the environment.

[0053] Specifically, the test tube culture temperature of the high-protein and high-activity yeast is 25-30° C., and the culture time is 12-24 hours; the shake flask culture temperature is 25-28° C., the rotation speed is 150-250 rpm, and the culture time is 12-24 hours;

[0054] Specifically, the production bacterial liquid contains 10 high-protein and high-activity yeast viable bacteria. 6 ~10 9 The pineapple is fresh pomace after peeling in a juice factory or canning factory without any processing, or fresh pineapple pomace is dried or treated with other antiseptic methods. The fermentation products are mixed in the step 4, and the moisture content thereof should be less than 15%;

[0055] Specifically, in the step 1, under the stirring condition of the seed tank, the fermentation temperature is 25-28° C. and the culture time is 24-36 hours, the seed liquid is obtained, and the seed liquid is inoculated into the fermentation tank at a 10% inoculation amount;

[0056] Specifically, in the step 3, ganoderma lucidum polysaccharide is added to the treated pineapple peel pomace, and the amount of the added ganoderma lucidum polysaccharide can be adjusted according to the specific formula to enhance the biological activity and nutritional value of the feed;

[0057] Specifically, in the step 1, the seed liquid is pretreated with the first biological enzyme, wherein the first biological enzyme is any one of cellulase or hemicellulase, so as to improve the degradation ability of microorganisms on pineapple peel pomace in the subsequent fermentation process;

[0058] Specifically, in the step three, the second biological enzyme is used to promote the decomposition of the difficult-to-degrade components in the pineapple peel pomace, and the second biological enzyme is any one of a protease or an amylase, so as to improve the fermentation efficiency and the feed quality;

[0059] Specifically, in the step 4, the dried fermentation product is extruded through a molding extruder, and pellet feed is produced using molds of different shapes and sizes to meet the needs of different animal feeding;

[0060] In the fourth step, the produced pellet feed is subjected to quality inspection, including protein content, moisture content and microbial index, to ensure that the product meets relevant standards and requirements, and the qualified products are packaged and marked with production date, shelf life and ingredient information for easy storage and sale;

[0061] Through the setting of the above technical solution,

[0062] Preparation method 1:

[0063] 20 tons of fresh pineapple peel pomace were fermented and its main components were: water content about 76%; dry matter about 24%; crude protein 6.55; crude ash 6.34; crude fiber 28.8; total sugar 1.16; calcium 2643; phosphorus 1342; pH value below 5.0, viable bacteria count 10 6 ~10 9 / ml high-protein and high-activity yeast liquid, Ganoderma lucidum polysaccharide, etc.

[0064] The high-protein and high-activity yeast strain (commercially available) was inoculated into a 1000 ml shake flask at a temperature of 25-28°C and a speed of 200 rpm. After 18 hours of culture, it was inoculated into a 1 ton seed tank with intermittent stirring at a temperature of 25-28°C. After 20 hours of culture, it was inoculated into a 10 ton fermentation tank with intermittent stirring at a temperature of 25-28°C. After 24 hours of culture, the number of viable bacteria per ml of the fermentation liquid reached 10 6 ~10 9 / ml, which is the mature production bacterial solution;

[0065] 1% to 5% of the bacterial liquid for production and 1% to 5% of the ganoderma lucidum polysaccharide are added to the peeled fresh pineapple peel pomace from a juice factory or a canning factory according to the weight of the pineapple peel pomace, crushed and stirred evenly, and then sealed and fermented at a temperature of 20 to 72° C. for 7×24 hours. The pH value of the pineapple peel pomace is detected to be below 5.0, and the fermentation is completed;

[0066] After fermentation, the water content of the pineapple peel pomace is 70% to 80%. After mixing (corn, fish meal, kelp powder, zeolite powder, oyster shell powder) to reduce the water content to below 15%, the protein feed can be extruded into particles of desired shape and size by an extrusion molding machine, and then weighed and packaged to become a biological enzyme protein feed.

[0067] Preparation method 2:

[0068] 20 tons of fresh pineapple peel pomace were fermented and its main components were: moisture about 76%; dry matter about 24%; crude protein 6.55; crude ash 6.34; crude fiber 28.8; total sugar 1.16; calcium 2643; phosphorus 1342; pH value below 5.0; viable bacteria count 10 6 ~10 9 / ml high-protein and high-activity yeast liquid, Ganoderma lucidum polysaccharide, etc.

[0069] The high-protein and high-activity yeast strain (commercially available) was inoculated into a 1000 ml shake flask at a temperature of 25-28°C and a speed of 200 rpm. After 18 hours of culture, it was inoculated into a 1 ton seed tank and intermittently stirred at a temperature of 25-28°C for 20 hours. After that, it was inoculated into a 10 ton fermentation tank with intermittent stirring at a temperature of 25-28°C for 24 hours. The number of viable bacteria per ml of fermentation liquid was 10 6 ~10 9 / ml, which is the mature production bacterial solution;

[0070] 1% to 5% of the bacterial liquid for production and 1% to 5% of the ganoderma lucidum polysaccharide are added to the fresh pineapple peel pomace according to the weight of the pineapple peel pomace, crushed, added with 20% of fish meal, stirred evenly, and then sealed and fermented at a temperature of 20 to 72° C. for 7×24 hours. When the crude protein content of the pineapple pomace is detected to be between 20 and 68, and the pH value is below 5.0, the fermentation is completed;

[0071] From the above, it can be seen that crushing the pineapple peel and sealing and fermenting it with biological protease and Ganoderma lucidum polysaccharide at a pH value below 5.0 can provide a low-cost and high-efficiency ecological feed solution for poultry farming. The specific advantages are as follows:

[0072] Pineapple peel is rich in cellulose, pectin, organic acid and natural bromelain, and can be used as a high-quality fermentation substrate after being crushed; exogenous proteases work together with pineapple endogenous enzymes to efficiently degrade crude fiber and anti-nutritional factors in pineapple peel, release soluble sugars (such as fructose, glucose) and small molecule peptides, and increase feed energy density; polysaccharides form complexes with organic acids in pineapple peel to enhance the antioxidant and immunomodulatory functions of feed;

[0073] Pineapple peel is a waste product of the juice processing industry. It can be obtained at almost zero cost and can replace 10% to 25% of corn or soybean meal, significantly reducing feed costs (estimated savings of 15% to 30%). The high moisture content of pineapple peel (70% to 80%) is suitable for wet-base sealed fermentation, without the need for additional humidity adjustment, thus reducing energy consumption.

[0074] The cellulose and hemicellulose in pineapple peel are converted into short-chain fatty acids (SCFAs) and soluble dietary fiber under the synergistic action of acid protease and lactic acid bacteria, which improves the fiber utilization rate of poultry (from less than 10% to 25% to 30%); the pectin and tannin in pineapple peel are degraded, reducing the inhibition of poultry intestinal digestive enzymes, reducing feed viscosity, and improving digestion rate;

[0075] Lactic acid bacteria produced by sealed fermentation (≥1×10 8 CFU / g) and fructooligosaccharides and ganoderma polysaccharide fragments in pineapple peel form a "prebiotic network" to promote the proliferation of beneficial intestinal bacteria (such as bifidobacteria) and inhibit Salmonella and Escherichia coli (the inhibition rate can reach 60% to 80%); Ganoderma polysaccharides can stimulate the secretion of mucin by intestinal goblet cells, strengthen the intestinal barrier function, and reduce the risk of parasitic infections such as coccidiosis;

[0076] The lactic acid (pH 3.5-4.0), bacteriocins (such as nisin) and antimicrobial peptide-like effects of ganoderma lucidum polysaccharides in the fermentation products form a triple antimicrobial defense line, which can reduce the use of antibiotics in poultry farming by 30%-50%; the organic acids (such as citric acid) and ganoderma lucidum polysaccharides in pineapple peels synergistically alleviate heat stress and improve poultry growth performance (daily weight gain increased by 8%-12% under high temperature environment);

[0077] Pineapple peel (after crushing) 70% + fish meal 20% + biological protease (acidic protease, 5000U / g) 0.1% + Ganoderma lucidum polysaccharide (purity ≥30%) 0.5% + lactic acid bacteria inoculant (5%);

[0078] The acidic sealed fermentation system of pineapple peel-bioprotease-Ganoderma lucidum polysaccharide provides a green, low-cost, high-return innovation path for poultry farming through the three-in-one effect of "waste value-added + nutrition fortification + antibiotic replacement and prevention". Its core value lies in:

[0079] Transform pineapple peel into highly active functional feed; the acidic environment is in line with the digestive physiology of poultry and improves nutrient utilization; reduces antibiotic pollution and promotes the development of circular agriculture.

[0080] By introducing the biological enzyme pretreatment technology, the present invention pretreats the pineapple peel pomace before fermentation, thereby reducing the content of the difficult-to-degrade components thereof, improving the degradation ability of the pineapple peel pomace by microorganisms, and at the same time, continuously utilizing the biological enzyme to promote the decomposition of the difficult-to-degrade components during the fermentation process, further improving the fermentation efficiency, thereby enabling the pineapple peel pomace to be more fully converted into high-quality protein feed;

[0081] The present invention adopts high-protein and high-activity yeast for fermentation, and combines the addition of ganoderma lucidum polysaccharide to significantly improve the protein content and nutritional value of the feed. At the same time, by optimizing the fermentation conditions and real-time monitoring and regulation technology, the stability and controllability of the fermentation process are ensured, thereby producing a protein feed with excellent quality and balanced nutrition.

[0082] Compared with the traditional feed production method, the present invention uses waste pineapple peel and pomace as raw materials, which not only reduces the production cost but also realizes the recycling of resources. In addition, through intelligent management and control technology, the production efficiency and product quality are improved, and the production cost is further reduced.

[0083] The implementation of the present invention reduces the amount of landfill and incineration of pineapple peel and pomace, reduces environmental pollution, and at the same time, the produced protein feed can be used for animal breeding, replacing part of traditional feed, reducing dependence on food resources, and helping to achieve sustainable agricultural development;

[0084] In summary, the bio-enzyme protein feed produced by the present invention has no chemical additive ingredients, has a natural fruit fermentation aroma, a protein content of about 20%, and contains a variety of amino acids, vitamins and trace elements, has strong palatability for livestock, and is a high-quality and low-cost nutritious protein feed. At the same time, the fresh pomace processed by the present invention has a simple process, and the selected equipment is a mature industrial complete set, and a large amount of pomace can be processed. The produced product has high nutritional value, strong palatability for livestock, and has no chemical additive ingredients. It is suitable for feeding various livestock, solves the current pollution problem of pomace processing in juice factories and canning factories, and the enterprises can increase considerable profits, with significant environmental benefits, economic benefits and social benefits. The present invention is suitable for the processing and transformation of pomace in various juice factories and canning factories, and controls the pollution to the environment.

[0085] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine different embodiments or examples described in this specification and the features of different embodiments or examples, unless they are contradictory.

[0086] In the drawings of the embodiments disclosed in the present invention, only the structures involved in the embodiments disclosed in the present invention are involved, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0087] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for producing bio-enzyme protein feed by using pineapple peel pomace through bio-enzyme fermentation, characterized in that: include: Step 1: The high-protein and high-activity yeast with a bacterial protein content of more than 40% is cultured in a test tube, a shake flask, or a seed tank, and then inoculated into a fermentation tank at a 10% inoculation rate and cultured for 24 hours to obtain a production bacterial liquid; Step 2: 1% to 5% of the production bacterial liquid and 1% to 5% of the commercially available finished ganoderma lucidum polysaccharide by weight of the pineapple peel pomace are crushed and evenly stirred with the pineapple peel pomace, and then put into a barrel of more than 10 tons, and then sealed and fermented for 7×24 hours, and the fermentation temperature is between 20 and 72°C; Step 3: adjusting the pH value of the fermented pineapple peel pomace to below 5.0, and packaging the obtained fermentation product with a breathing bag and storing it in a cool place for later use; In the step three, it also includes taking samples from the mixture after the enzymatic hydrolysis reaction to test indicators such as protein content, amino acid composition, enzyme activity, ganoderma polysaccharide, oligosaccharide content, etc., and evaluating the quality of the finished product based on the test results. The evaluation content includes protein conversion rate, amino acid balance, ganoderma polysaccharide activity, oligosaccharide content, etc. to ensure that the quality of the finished product meets the predetermined standards and requirements; In the step 3, the qualified finished products are packaged, and the sealing, light-proof and moisture-proof properties of the packaging materials should be ensured during the packaging process; After packaging, store the finished products in a dry, cool, well-ventilated storage environment to avoid the effects of high temperature and humidity on the quality of the finished products; Step 4: Mix the fermentation product obtained in the above step 3 with corn, fish meal, kelp powder, zeolite powder and oyster shell powder, and extrude the mixture into pellet feed through a forming extruder; In step 4, reasonable usage specifications are formulated based on the nutritional requirements and digestive capacity of different poultry and aquatic animals, and the usage should be adjusted according to factors such as the animal's growth stage, weight and breeding environment.

2. A method for producing bio-enzyme protein feed by bio-enzyme fermentation using pineapple peel pomace according to claim 1, characterized in that: The test tube culture temperature of the high-protein and high-activity yeast is 25-30° C., and the culture time is 48 hours; the shaking flask culture temperature is 25-28° C., the rotation speed is 150-250 rpm, and the culture time is 12-24 hours.

3. A method for producing bio-enzyme protein feed by bio-enzyme fermentation using pineapple peel pomace according to claim 1, characterized in that: The production bacterial liquid contains 10 high-protein and high-activity yeast live bacteria 6 ~10 9 The pineapple peel pomace is fresh pomace without any processing after peeling in the juice factory. After the fermentation products are mixed in the step 4, the moisture content thereof should be less than 15%.

4. A method for producing bio-enzyme protein feed by bio-enzyme fermentation using pineapple peel pomace according to claim 1, characterized in that: In the step one, under the stirring condition of the seed tank, the fermentation temperature is 25-28°C and the culture time is 24-36 hours to obtain the seed liquid, and the seed liquid is inoculated into the fermentation tank at a 10% inoculation amount.

5. A method for producing bio-enzyme protein feed by bio-enzyme fermentation using pineapple peel pomace according to claim 1, characterized in that: In the step 2, ganoderma lucidum polysaccharide is added to the processed pineapple peel pomace, and the added amount can be adjusted according to the specific formula to enhance the biological activity and nutritional value of the feed.

6. A method for producing bio-enzyme protein feed by bio-enzyme fermentation using pineapple peel pomace according to claim 1, characterized in that: In the step 1, the seed liquid is pretreated with the first biological enzyme, which is any one of cellulase and hemicellulase, so as to improve the degradation ability of microorganisms on pineapple peel pomace in the subsequent fermentation process.

7. A method for producing bio-enzyme protein feed by bio-enzyme fermentation using pineapple peel pomace according to claim 1, characterized in that: In the step 2, the second biological enzyme is used to promote the decomposition of the difficult-to-degrade components in the pineapple peel pomace, and the second biological enzyme is any one of a protease or an amylase, so as to improve the fermentation efficiency and the feed quality.

8. The method for producing bio-enzyme protein feed by bio-enzyme fermentation using pineapple peel pomace according to claim 1, characterized in that: In the step 4, the mixed fermentation product is extruded through a molding extruder, and pellet feed is produced using molds of different shapes and sizes to meet the needs of different animal feeding; In step 4, the quality of the pellet feed produced is tested, including protein content, moisture content and microbial indicators, to ensure that the product meets relevant standards and requirements, and the qualified products are packaged and marked with production date, shelf life and ingredient information for easy storage and sale.

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