Phytobacterium plantarum YW99, cell lysate thereof and antibacterial application of phytobacterium plantarum YW99 and cell lysate

Abstract

The invention relates to the field of microorganisms, in particular to a plant lactobacillus YW99 strain, a cell lysate of the plant lactobacillus YW99 strain and antibacterial application of the plant lactobacillus YW99 strain and the cell lysate. The lactobacillus plantarum YW99 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lactobacillus plantarum YW99 is CGMCC No.33160. The lactobacillus plantarum YW99 has the advantages that the lactobacillus plantarum YW99 is preserved in the China General Microbiological Culture Collection Center (CGMCC); the plant lactobacillus YW99 cell lysate is prepared by heating and inactivating a YW99 strain and then carrying out a series of operations, has good safety, does not generate toxic and side effects on a human body, has a certain function of improving the immunity of the organism, and has a good application prospect. And by changing the concentration of the YW99 cell lysate, the YW99 cell lysate can show different degrees of bacteriostatic ability to candida albicans, gardnerella, pseudomonas aeruginosa, aspergillus brazilis and other strains, and has a very good application prospect in female health products, hygienic products, daily chemical products and disinfection products.

Description

Technical Field

[0001] The invention relates to the field of microorganisms, in particular to a strain of Lactobacillus plantarum YW99 and a cell lysate thereof and antibacterial applications of the two. Background Art

[0002] As people's living standards improve, they are not only satisfied with food and clothing, but also pay more attention to health. Pathogenic bacteria are bacteria that cause human diseases. They are tiny and can enter the human body through various pathways, parasitize, proliferate and cause diseases in the human body.

[0003] Pathogenic bacteria such as Candida albicans and Gardnerella can attach to the skin or mucous membranes of the human body. When the balance of the human flora is broken, these pathogens will multiply in large numbers and cause diseases. They are the main pathogens of vaginitis and the primary factor causing many common female health problems. However, there are not many targeted effective substances and the effects are not obvious. Among the common existing technologies on the market, most female hygiene products with antibacterial and antibacterial functions use antibacterial agents containing metals, antibiotics or organic matter: First, during use, these antibacterial agents are highly irritating to the skin and mucous membranes, which will cause certain discomfort to the applicable population, or have defects such as carcinogenicity, teratogenicity, and short validity period, affecting physical and mental health and normal life; secondly, the antibacterial agent will be lost, which will not only reduce the antibacterial efficiency and timeliness of female hygiene products, but also pollute the environment.

[0004] Pseudomonas aeruginosa, also known as Pseudomonas aeruginosa, is a conditionally pathogenic bacterium that is resistant to multiple antibiotics and is a common contaminant of food and cosmetics. There are currently no good antimicrobial drugs, and it is mainly inhibited by the use of antibiotics. However, Pseudomonas aeruginosa is very prone to drug resistance, so there are few effective antibiotics. However, excessive use will cause bacterial resistance to become more serious, posing a potential threat to human health and the ecological environment.

[0005] Aspergillosis is a chronic fungal disease caused by infection with Aspergillus, which can invade the skin, mucous membranes, eyes, external auditory canal, nose, sinuses, bronchi, lungs, gastrointestinal tract, nervous system or bones, and in severe cases lead to sepsis. Brazilian Aspergillus is the most common type of Aspergillus fungus, which can be spread in production sites or hospitals through improperly cleaned equipment, and can cause serious lung lesions after infection. Common inhibitory drugs are broad-spectrum disinfectants and antifungal agents, the former are used for industrial disinfection, and the latter are used to treat infected people.

[0006] Natural antibacterial preservatives are mainly derived from animals, plants, microbial extracts, etc. They are safer for the human body and meet green environmental protection requirements. Due to its safety, lactic acid bacteria have been widely used in the food field, and the metabolites of lactic acid bacteria, bacteriocins, are currently natural antibacterial agents that combine safety and antibacterial activity, and have attracted much attention. For example, reuterin has extremely strong antibacterial activity against Helicobacter pylori, and nisin has a certain inhibitory effect on some foodborne Gram-positive pathogens, but in the fields of women's health products, sanitary products, daily chemical products, disinfection products, etc., it still cannot meet the increasingly diversified market demand and the antibacterial and antibacterial needs of the female population, which not only restricts the high-quality development of various industries, but also affects people's physical health. Therefore, it is urgent to find a new, natural, safe, broad-spectrum antibacterial and efficient antibacterial substance, which can be used in women's health products, sanitary products, daily chemical products, disinfection products and other fields to promote the upgrading of the industry. Summary of the invention

[0007] In view of the deficiencies in the above-mentioned prior art, the present application provides a strain of Lactobacillus plantarum YW99 and a cell lysate of Lactobacillus plantarum YW99.

[0008] The first object of the present invention is to provide an application of Lactobacillus plantarum in preparing a product with antibacterial function, wherein the Lactobacillus plantarum is Lactobacillus plantarum YW99, which is classified and named Lactiplantibacillus plantarum , deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 23, 2024, with the deposit number CGMCC No.33160. The product is one or more of women's health products, sanitary products, daily chemical products, and disinfection products.

[0009] Furthermore, the above-mentioned antibacterial function includes at least one of the following: (1) inhibiting the growth of Candida albicans; (2) inhibiting the growth of Gardnerella; (3) inhibiting the growth of Pseudomonas aeruginosa; (4) inhibiting the growth of Aspergillus brasiliensis.

[0010] Furthermore, the above-mentioned products meet at least one of the following conditions: (1) sanitary products are gynecological hygiene products, which are sanitary napkins and gel products; (2) daily chemical products are oral health care products, which are one or more of mouthwash, mouthwash powder, toothpaste, tooth powder, and mouth spray; (3) disinfectant products are skin disinfectant products, which are one or more of paper towels, wet wipes, antibacterial liquid products, wet toilet paper, and facial masks.

[0011] The second object of the present invention is to provide an application of a Lactobacillus plantarum YW99 cell lysate solution in the preparation of a product with antibacterial function, wherein the Lactobacillus plantarum YW99 cell lysate solution is prepared from Lactobacillus plantarum YW99 with a deposit number of CGMCC No. 33160, and the Lactobacillus plantarum YW99 cell lysate solution satisfies at least one of the following conditions: (1) the concentration of the Lactobacillus plantarum YW99 cell lysate solution is 10% (W / V), and at least 10 10 CFU / mL of inactivated Lactobacillus plantarum YW99 bacteria; (2) including metabolites of Lactobacillus plantarum YW99, lactic acid content ≥22mg / 100mL, and acetic acid content ≥5mg / 100mL; (3) including flavonoids, metabolites of Lactobacillus plantarum YW99, with a flavonoid content ≥52mg / mL; (4) including amino acids, metabolites of Lactobacillus plantarum YW99, with an amino acid content ≥70µmol / mL; (5) including polysaccharides, metabolites of Lactobacillus plantarum YW99, with a polysaccharide content ≥700mg / L; the product is one or more of women's health products, sanitary products, daily chemical products, and disinfection products.

[0012] Furthermore, the above-mentioned antibacterial function includes at least one of the following: (1) inhibiting the growth of Candida albicans; (2) inhibiting the growth of Gardnerella; (3) inhibiting the growth of Pseudomonas aeruginosa; (4) inhibiting the growth of Aspergillus brasiliensis.

[0013] Furthermore, the above-mentioned products meet at least one of the following conditions: (1) sanitary products are gynecological hygiene products, which are sanitary napkins and gel products; (2) daily chemical products are oral health care products, which are one or more of mouthwash, mouthwash powder, toothpaste, tooth powder, and mouth spray; (3) disinfectant products are skin disinfectant products, which are one or more of paper towels, wet wipes, antibacterial liquid products, wet toilet paper, and facial masks.

[0014] Compared with the prior art, the present invention has the following advantages: (1) The present application provides a strain of Lactobacillus plantarum YW99 and a cell lysate of Lactobacillus plantarum YW99. The strain is isolated and screened from Tibetan kefir grains and is a probiotic with strong antibacterial function. It has certain application value in the preparation of women's health products, sanitary products, daily chemical products (including but not limited to dental products, cosmetics), and disinfection products with antibacterial function. The cell lysate of Lactobacillus plantarum YW99 is prepared by heat inactivation, and the preparation method is simple and efficient. The prepared cell lysate of Lactobacillus plantarum YW99 has a good inhibitory effect on the growth of pathogenic bacteria such as Candida albicans, Gardnerella, Pseudomonas aeruginosa, and Aspergillus braziliensis. The preparation method can increase the concentration of effective substances in the cell lysate of Lactobacillus plantarum YW99 and enhance the antibacterial effect of the cell lysate of Lactobacillus plantarum YW99 on Candida albicans, Gardnerella, Pseudomonas aeruginosa, and Aspergillus braziliensis.

[0015] (2) Based on the function of Lactobacillus plantarum YW99 cell lysate, this application applies Lactobacillus plantarum YW99 or Lactobacillus plantarum YW99 cell lysate to the fields of women's health products, sanitary products, daily chemical products (including but not limited to dental products, cosmetics), disinfection products, etc., providing the market and consumers with a natural and highly safe antibacterial option. Compared with chemical antibacterial and antibacterial drugs, it has the characteristics of being natural, safe and non-toxic, and having good biocompatibility. On the premise of ensuring antibacterial efficiency and timeliness, it reduces the irritation to the user's skin and mucous membranes, as well as the probability of carcinogenicity and teratogenicity, and on the other hand, it meets the requirements of green environmental protection and has a wider range of applications; compared with antibiotic agents, there is no risk of excessive use causing bacterial resistance; compared with traditional probiotic preparations, Lactobacillus plantarum YW99 cell lysate is non-pathogenic and its metabolites are released more completely. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a diagram showing the effect of the YW99 strain cell lysate of Experimental Example 1 of the present application on inhibiting Candida albicans; Figure 2 This is a diagram showing the effect of the YW99 strain cell lysate of Experimental Example 1 of the present application on inhibiting Gardnerella; Figure 3 This is a diagram showing the effect of the YW99 strain cell lysate of Experimental Example 1 of the present application on inhibiting Pseudomonas aeruginosa; Figure 4 The MIC test results of the YW99 strain cell lysate against Candida albicans in Experimental Example 2 of this application; Figure 5 This is the antiseptic test result of the YW99 antibacterial gel of Test Example 4 of this application. DETAILED DESCRIPTION

[0017] The present invention will be further described below in conjunction with specific embodiments. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and cannot be used to limit the scope of protection of the present invention. Anything not described in detail in the present patent application can be understood as common knowledge in the art.

[0018] With the development of society, consumers have more diverse requirements for women's health products, sanitary products, daily chemical products (including but not limited to dental products, cosmetics), and disinfection products with antibacterial and antimicrobial functions. A large number of studies have shown that probiotics have a variety of probiotic functions, such as regulating intestinal flora, enhancing immunity, promoting nutrient absorption, lowering blood sugar, anti-oxidation, and reducing disease risks. Lactobacillus cell lysate, as a preparation of inanimate microorganisms and / or their components that are beneficial to host health, mainly includes metabolites of probiotic bacteria, as well as soluble substances produced after the bacteria are eliminated and decomposed, such as bacteriocins, antimicrobial peptides, organic acids, and extracellular polysaccharides. Compared with probiotics, it is more stable and safer, and is also suitable for some special populations such as newborns and sensitive populations, and is not interfered with or inhibited by antibiotics. However, at present, probiotics and lactic acid bacteria cell lysates are mostly used in the food field and are rarely used in the disinfection and antibacterial fields. Therefore, this application aims to apply probiotics and / or lactic acid bacteria cell lysates to women's health products, sanitary products, daily chemical products (including but not limited to dental chemical products, cosmetics), disinfection products and other industries to provide consumers with a new, natural, safe, broad-spectrum antibacterial and efficient antibacterial and antimicrobial product.

[0019] The plant lactobacillus used in the present invention is: plant lactobacillus YW99, the preservation date is December 23, 2024, the preservation unit is the General Microbiology Center of the China Microbiological Culture Collection Administration, and the classification name is plant lactobacillus ( Lactiplantibacillus plantarum ), the deposit number is CGMCC No.33160.

[0020] Unless otherwise specified, the experimental methods in the following examples are all conventional methods; the experimental materials and culture media used in the following examples are all purchased from commercial channels unless otherwise specified.

[0021] MRS medium: glucose 20.0 g / L, peptone 10.0 g / L, beef extract 10.0 g / L, yeast extract 5.0 g / L, sodium acetate 5.0 g / L, diammonium hydrogen citrate 2.0 g / L, dipotassium hydrogen phosphate 2.0 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, 1.0 g / L Tween-80, distilled water to 1,000 mL, solid culture medium with 2% agar, pH 6.2-6.4, autoclaved at 115°C for 15 min.

[0022] Sabouraud dextrose agar (SDA): 40.0 g glucose, 5.0 g animal tissue gastric digest, 5.0 g tryptone, 15.0 g agar, distilled water to 1,000 mL, pH 5.4-5.8, autoclave at 121°C for 15 min.

[0023] Potato dextrose agar (PDA): 200.0 g potato extract, 20.0 g glucose, 20.0 g agar, distilled water to 1,000 mL, pH 5.4-5.8, autoclave at 121°C for 15 min.

[0024] Example 1 Preparation of YW99 cell lysate Pick an appropriate amount of Lactobacillus plantarum YW99 isolated from Tibetan kefir grains to prepare YW99 cell lysate, the specific method is as follows: S1. Inoculate Lactobacillus plantarum YW99 into MRS medium for full activation, and culture at 37°C for 24 hours until the end of the logarithmic phase to obtain an activated strain of YW99; then inactivate at 105°C for 20 minutes, centrifuge at 8,000 r / min for 3 minutes, and collect the supernatant to obtain a YW99 cell lysis stock solution, which is stored at -20°C for later use; S2. Prefreeze the YW99 cell lysate stock solution at -80°C for 3 h, and then perform freeze-drying operation for 24 h to obtain dry powdered YW99 cell lysate, which is stored for later use.

[0025] Example 2 Preparation of YW99 cell lysate solution The YW99 cell lysate obtained in Example 1 was resuspended to obtain a YW99 cell lysate solution with a concentration of 10% (W / V). The component was detected, and it was found that the 10% YW99 cell lysate solution contained metabolites such as lactic acid, acetic acid, flavonoids, amino acids and polysaccharides. The content detection results of the main metabolites are shown in Table 1.

[0026] Table 1 Component content in 10% YW99 cell lysate solution

[0027] In some specific embodiments, the 10% YW99 cell lysate solution can be added as a raw material to women's health products, sanitary products, daily chemical products (including but not limited to dental products, cosmetics), and disinfection products. In other specific embodiments, the pH of the final 10% YW99 cell lysate solution is in the range of 3.5-4.0, and the content of the bacteria and metabolites at least meets the following requirements: the inactivated Lactobacillus plantarum YW99 bacteria ≥ 10 10CFU / mL, lactic acid ≥22mg / 100mL, acetic acid ≥5mg / 100mL, flavonoids ≥52mg / mL, amino acids ≥70µmol / mL, polysaccharides ≥700mg / L. In other specific embodiments, the YW99 cell lysate solution may also contain the above metabolites alone or simultaneously to meet different application requirements.

[0028] Experimental Example 1 Qualitative detection of antibacterial ability of YW99 cell lysate In this test example, the YW99 cell lysate prepared in Example 1 was prepared into a solution with a concentration of 10% (W / V) for antibacterial performance determination. Activated Candida albicans ATCC 10231, Gardnerella, and Pseudomonas aeruginosa ATCC9027 were taken and prepared at a concentration of 1×10 8 CFU / mL of bacterial solution, 100 μL of each bacterial solution was spread on the corresponding solid culture medium, and holes were punched on the solid culture medium with a 6 mm inner diameter puncher, 4 or 5 holes per dish, 2 of which were used as positive control (+: 50 μg / mL ampicillin) and negative control (-: saline water), and YW99 cell lysate solution of different concentrations was punched into the wells at a content of 100 μL per well, and cultured at a certain temperature for a period of time, and the diameter of the inhibition zone was measured with a vernier caliper. The analysis results are as follows.

[0029] 1. Detection of the inhibitory ability of YW99 cell lysate against Candida albicans by the inhibition zone method Using Candida albicans as the experimental bacteria, the inhibition zone method was used. In this test example, the YW99 cell lysate prepared in Example 1 was prepared into solutions with concentrations of 10% (W / V) and 20% (W / V), and the antibacterial performance was measured respectively to measure the antibacterial ability of the two solutions against Candida albicans. The solid culture medium was SDA solid culture medium, and the culture was carried out at 35°C for 16h-18h. The inhibition zone results of the 10% YW99 cell lysate solution are shown in Figure 2. Figure 1 As shown in Table 2, the specific experimental data show that the diameters of the inhibition zones are 1.7 cm and 2.0 cm, respectively, indicating that the YW99 cell lysate has an inhibitory effect on Candida albicans.

[0030] Table 2 Diameters of inhibition zones of YW99 cell lysates against Candida albicans

[0031] The above results show that the YW99 cell lysate and the resuspended solution showed obvious inhibitory effect on Candida albicans. As the concentration of the YW99 cell lysate solution increased, its antibacterial effect on Candida albicans also increased significantly.

[0032] 2. Detection of the inhibitory ability of YW99 cell lysate against Gardnerella by the inhibition zone method The culture medium is BHI solid culture medium: purchased from Qingdao Haibo Biotechnology Co., Ltd., sterilized at 121℃ for 15min. Cultured at 35℃ for 24h-48h. The inhibition zone results of 10% YW99 cell lysate solution are as follows Figure 2 As shown, the diameter of the inhibition zone was 1.2 cm ± 0.2 cm, indicating that the YW99 cell lysate had an inhibitory effect on Gardnerella.

[0033] 3. Detection of the inhibitory ability of YW99 cell lysate against Pseudomonas aeruginosa by the inhibition zone method The solid culture medium was LB agar medium, and the culture was carried out at 37°C for 16h-18h. The inhibition zone results of 10% YW99 cell lysate solution were as follows Figure 3 As shown, the diameter of the inhibition zone was 1.6 cm ± 0.06 cm, indicating that the YW99 cell lysate had a good inhibitory effect on Pseudomonas aeruginosa.

[0034] Experimental Example 2 Quantitative detection of antibacterial ability of YW99 cell lysate This test example measured the minimum inhibitory concentration (MIC) of the YW99 cell lysate prepared in Example 1 against Candida albicans. The experiment was carried out using the microbroth dilution method in accordance with the "Health Industry Standard of the People's Republic of China" (WS / T639-2018). 6 CFU / mL of Candida albicans liquid was added to a 96-well plate at a volume of 100 μL / well, and the culture medium was used as the growth control group. The experimental group prepared different concentrations of YW99 cell lysate solution, including: 2.5% YW99 cell lysate solution, 5% YW99 cell lysate solution, 10% YW99 cell lysate solution, 20% YW99 cell lysate solution, and 3 parallels were set up for each group. The incubation conditions were 25℃-40℃ for 16h-24h. After the incubation, the turbidity of the liquid in the 96-well plate was observed to determine the lowest concentration of lactic acid bacteria cell lysate that inhibited the visible growth of pathogenic bacteria as MIC. The experimental results are as follows Figure 4 As shown, by naked eye observation, compared with the control group, with the increase of the concentration of YW99 cell lysate solution, the inhibitory effect on Candida albicans gradually increased. When the concentration of YW99 cell lysate solution was 5%, the suspension in the well was clear and no colonies were generated at the bottom of the well, indicating that YW99 cell lysate had a significant inhibitory effect on Candida albicans, and its minimum inhibitory concentration was 5%.

[0035] Experimental Example 3 Antibacterial ability of YW99 cell lysate was tested using standard Since the YW99 cell lysate has a strong antibacterial function against Candida albicans and Gardnerella, it has the basis and prospect for application in the preparation of disposable sanitary products and other products. Therefore, this test example was tested in accordance with GB 15979-2002 "Hygiene Standard for Disposable Sanitary Products" to verify its antibacterial properties as a raw material for the production of antibacterial products.

[0036] According to the requirements of GB15979-2002, this test example determines the inhibition rate of 10% YW99 cell lysate solution on Candida albicans at different action times. Take 5 mL of the sample to be tested (10% YW99 cell lysate solution) and 5 mL of the control solution (normal saline) in 5 tubes respectively, add 100 μL of Candida albicans suspension to each sample solution and control solution and mix them evenly. Start timing after mixing, and dilute and apply the sample solution and control solution at 2 min, 5 min, 10 min, and 20 min of action respectively. Set one repetition for each group of tests. Calculate the inhibition rate at different action times according to the following formula.

[0037]

[0038] Where: A is the average colony count in the control solution, and B is the average colony count in the sample to be tested.

[0039] According to the evaluation criteria of GB 15979-2002, if the inhibition rate is ≥50%-90%, the tested product has an antibacterial effect, and if the inhibition rate is ≥90%, the tested product has a strong antibacterial effect. The test results are shown in Table 3. It can be seen from Table 3 that the 10% YW99 cell lysate solution showed a strong antibacterial effect on Candida albicans at 2min, 5min, 10min, and 20min, and had an inhibitory effect at 2min.

[0040] Table 3 Antibacterial rate of YW99 cell lysates

[0041] The results in Table 3 show that adding YW99 cell lysate as a raw material in the process of preparing disposable sanitary products can improve the antibacterial effect of the product. In other specific embodiments, disposable sanitary products can be various daily necessities that are discarded after one use, directly or indirectly contact the human body, and are used to achieve the purpose of human physiological hygiene or health care (antibacterial or antibacterial). The product properties can be solid or liquid, including but not limited to paper towels, wet wipes, sanitary wet wipes, women's menstrual hygiene products, diapers and other excrement hygiene products, and condoms.

[0042] Example 3 Application of YW99 cell lysate in women's health products and hygiene products Because plant lactobacillus YW99 and / or plant lactobacillus YW99 cell lysate have strong antibacterial effect, it is added as raw material in suitable products to play the role of preservation. In some embodiments, plant lactobacillus YW99 and / or plant lactobacillus YW99 cell lysate can be used to be directly or indirectly added to women's health products to inhibit the growth of Candida albicans, Gardnerella, and Pseudomonas aeruginosa. In other embodiments, plant lactobacillus YW99 and / or YW99 cell lysate can also be added to sanitary products, including but not limited to gynecological hygiene products such as sanitary napkins and gel products.

[0043] This embodiment provides an application of a YW99 cell lysate in a female health product, and specifically relates to applying the YW99 cell lysate to prepare a gel-like gynecological hygiene product.

[0044] A YW99 antibacterial gel product, the preparation method of which is specifically as follows: adding 3% carbomer (Carbomer 934p powder produced by Anhui Newman Fine Chemical Co., Ltd.) to the 10% YW99 cell lysate solution prepared in Example 2, slowly stirring to completely dissolve it, standing for a period of time to fully hydrate, adjusting the pH to 4.2-4.5, to obtain the YW99 antibacterial gel.

[0045] Test Example 4: Antibacterial and antiseptic properties of YW99 antibacterial gel This test example tests the antibacterial and antiseptic properties of the YW99 antibacterial gel prepared in Example 3 against Candida albicans, Pseudomonas aeruginosa, and Aspergillus brasiliensis. Candida albicans was cultured on Sabouraud dextrose agar (SDA) and Aspergillus brasiliensis was cultured on potato dextrose agar (PDA). The concentration of pathogenic bacteria was 5×10 5 CFU / mL-4.5×10 6 CFU / mL. The test method is based on the international standard ISO 11930, with a detection period of 28 days. The evaluation criteria are shown in Table 4.

[0046] Table 4 Evaluation criteria

[0047] The results of the antibacterial effect test of YW99 antibacterial gel are as follows Figure 5As shown in the figure, T0, T7, T14, and T28 represent the sampling time on the 0th, 7th, 14th, and 28th days, respectively, and N0, N7, N14, and N28 represent the total number of bacteria in the samples at different sampling times. The results show that on the 14th day of culture, the number of viable bacteria of Candida albicans and Aspergillus brasiliensis in the YW99 antibacterial gel is less than 10. According to the formula in Experimental Example 3, the logarithmic reduction values ​​R14 of the number of surviving bacteria are 4.6 and 4.6, respectively, and the experimental results meet the standard B. On the 28th day of culture, the number of surviving bacteria of the mixed gel against Pseudomonas aeruginosa, Candida albicans, and Aspergillus brasiliensis is less than 10. According to the formula in Experimental Example 3, the logarithmic reduction values ​​R28 of the number of surviving bacteria are 5.7, 4.6, and 4.6, respectively.

[0048] By comparing the results on the 7th, 14th and 28th days with standard B, it is known that YW99 antibacterial gel meets standard B in the international standard (ISO 11930), that is, it has antiseptic and antibacterial effects on Pseudomonas aeruginosa, Candida albicans and Aspergillus brasiliensis.

[0049] In summary, it can be seen that YW99 cell lysate can be used as a raw material for antibacterial products in women's health products to inhibit or prevent the reproduction of pathogenic bacteria such as Pseudomonas aeruginosa, Candida albicans and Aspergillus brasiliensis, and exert an antibacterial effect.

[0050] Example 4 Application of YW99 cell lysate in daily chemical products and disinfectants Since the human oral cavity naturally contains various bacteria, fungi and other microbial flora, and has suitable temperature and humidity, it is very easy to promote the reproduction of microorganisms. In some embodiments, Lactobacillus plantarum YW99 and / or YW99 cell lysate can be added to daily chemical products, including but not limited to oral health care products such as mouthwash, mouthwash powder, toothpaste, tooth powder, mouth spray, etc.

[0051] In other embodiments, Lactobacillus plantarum YW99 and / or YW99 cell lysate can also be added to disinfection products, including but not limited to disinfectants, skin disinfection products (paper towels, wet wipes, antibacterial liquid products, wet toilet paper), etc. In some embodiments, the final dosage form of the antibacterial product added with Lactobacillus plantarum YW99 and / or Lactobacillus plantarum YW99 cell lysate can be any one of aerosols, sprays, films, ointments, suppositories, pastes, solutions, gels, injections, and lotions.

[0052] The above is based on the ideal embodiment of the present invention. Through the above description, relevant personnel can make various changes and modifications without departing from the technical concept of the present invention. The technical scope of the present invention is not limited to the content in the specification, and the technical scope must be determined according to the scope of the claims.

Claims

1. An application of Lactobacillus plantarum in preparing a product with antibacterial function, characterized in that: The plant lactobacillus is plant lactobacillus YW99, which is classified and named Lactiplantibacillus plantarum , deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 23, 2024, with the deposit number CGMCC No.33160. The product is one or more of women's health products, sanitary products, daily chemical products, and disinfection products.

2. The use according to claim 1, characterized in that: The antibacterial function includes at least one of the following: (1) Inhibit the growth of Candida albicans; (2) Inhibit the growth of Gardnerella; (3) Inhibit the growth of Pseudomonas aeruginosa; (4) Inhibit the growth of Aspergillus brasiliensis.

3. The use according to claim 2, characterized in that: The product meets at least one of the following requirements: (1) The sanitary products are gynecological sanitary products, and the gynecological sanitary products are sanitary napkins and gel products; (2) The daily chemical product is an oral health care product, and the oral health care product is one or more of mouthwash, mouthwash powder, toothpaste, tooth powder, and mouth spray; (3) The disinfectant is a skin disinfectant, which is one or more of paper towels, wet wipes, antibacterial liquid products, wet toilet paper, and facial masks.

4. An application of a Lactobacillus plantarum YW99 cell lysate solution in the preparation of a product with antibacterial function, characterized in that: The Lactobacillus plantarum YW99 cell lysate solution is prepared from Lactobacillus plantarum YW99 with a deposit number of CGMCC No. 33160, and the Lactobacillus plantarum YW99 cell lysate solution at least satisfies one of the following conditions: (1) The concentration of the Lactobacillus plantarum YW99 cell lysate solution is 10% (W / V), including at least 10 10 CFU / mL of inactivated Lactobacillus plantarum YW99 bacteria; (2) Lactic acid and acetic acid, metabolites of Lactobacillus plantarum YW99, wherein the lactic acid content is ≥22 mg / 100 mL and the acetic acid content is ≥5 mg / 100 mL; (3) including flavonoids metabolites of Lactobacillus plantarum YW99, wherein the flavonoid content is ≥ 52 mg / mL; (4) Amino acids comprising metabolites of Lactobacillus plantarum YW99, wherein the amino acid content is ≥ 70 µmol / mL; (5) Polysaccharides comprising metabolites of Lactobacillus plantarum YW99, wherein the content of the polysaccharides is ≥ 700 mg / L; The products are one or more of women's health products, sanitary products, daily chemical products, and disinfection products.

5. The use according to claim 4, characterized in that: The antibacterial function includes at least one of the following: (1) Inhibit the growth of Candida albicans; (2) Inhibit the growth of Gardnerella; (3) Inhibit the growth of Pseudomonas aeruginosa; (4) Inhibit the growth of Aspergillus brasiliensis.

6. The use according to claim 5, characterized in that: The product meets at least one of the following requirements: (1) The sanitary products are gynecological sanitary products, and the gynecological sanitary products are sanitary napkins and gel products; (2) The daily chemical product is an oral health care product, and the oral health care product is one or more of mouthwash, mouthwash powder, toothpaste, tooth powder, and mouth spray; (3) The disinfectant is a skin disinfectant, which is one or more of paper towels, wet wipes, antibacterial liquid products, wet toilet paper, and facial masks.

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