A PDO barbed suture with an antibacterial coating and its preparation method

Abstract

This application relates to the field of medical suture technology, specifically disclosing a PDO barbed suture with an antibacterial coating and its preparation method. The PDO barbed suture with an antibacterial coating is prepared by blending and spinning a PDO mixed suture matrix with polylactic acid (PLA) and polydioxanone (PD-dioxanone) in a certain proportion to obtain a PDO mixed suture matrix with good mechanical properties. Then, using hyaluronic acid as a gel matrix, 4-(ethylene oxide-2-ylmethoxy)butyl acrylate is grafted onto the hyaluronic acid for alkenyl modification. Next, it is mixed with quercetin-3-O-glucoside and TCS@ZIF-8 loaded with oridonin to obtain an antibacterial gel coating. Finally, the PDO mixed suture matrix is ​​impregnated in the antibacterial gel coating and prepared by ultraviolet light irradiation under a photoinitiator. The suture obtained by this application has the advantages of a smooth coating, highly efficient and durable antibacterial properties, high strength, good flexibility, low friction, and high operability.

Description

Technical Field

[0001] This application relates to the field of medical suture technology, and more specifically, to a PDO barbed suture with an antibacterial coating and a method for preparing the same. Background Technology

[0002] Biodegradable sutures have been widely studied due to their significant advantages, such as eliminating the need for suture removal during wound healing and the non-toxic and harmless degradation products. Polydioxanone (PDO), with its excellent biodegradability, bioabsorbability, biocompatibility, softness, and ease of processing, is often used in the manufacture of biodegradable surgical sutures. However, traditional PDO sutures generally employ a braided structure, and the resulting uneven surface easily attracts bacteria, causing surgical site infections. This can lead to varying degrees of suppuration, inflammation, and lesions in the wound. Therefore, antibacterial treatment of the sutures is necessary.

[0003] However, existing absorbable sutures all have various problems in clinical use. For example, Chinese patent CN204275113U uses polycaprolactone loaded with the antibacterial drug levofloxacin hydrochloride to prepare an antibacterial coating solution, coating the braided sutures to obtain a medical suture with good antibacterial and sustained-release properties. However, excessive use of antibiotics poses certain risks to the human body. Chinese patent CN105107016A discloses a highly antibacterial absorbable medical suture using carboxymethyl chitosan, polyhydroxyacetic acid, hyaluronic acid, silk fibroin, salmon collagen, glutaraldehyde, genipin, and glycosides. The suture was prepared using mannitol, glycerin, and a coating. Although the prepared suture has good antibacterial properties, it suffers from a short antibacterial time in the later stages. Chinese patent CN107213507A discloses an absorbable suture and its preparation method. The suture is composed of polyglycolic acid (PGA), calcium stearate, and chitosan and is prepared by a weaving process. Although it has good tensile strength and antibacterial properties, the woven suture is relatively rough and easily drags and damages tissues. It also causes continuous friction and wear to the contacting tissues after surgery.

[0004] Therefore, developing a suture with excellent mechanical properties and durable antibacterial properties can effectively prevent the risk of surgical site infection. Summary of the Invention

[0005] To further improve the ability of sutures to prevent the risk of surgical site infection, this application provides a PDO barbed suture with an antibacterial coating and a method for preparing the same.

[0006] In a first aspect, this application provides a method for preparing PDO barbed sutures with an antibacterial coating, using the following technical solution:

[0007] A method for preparing PDO barbed sutures with an antibacterial coating includes the following steps:

[0008] S1, Preparation of PDO mixed suture matrix: Polylactic acid (PLA) and polydioxanone (PD-dioxanone) are mixed in proportion, melted and mixed at 150-170℃ for 10-20 min, spun and stretched, and woven with a braiding machine to obtain PDO mixed suture matrix;

[0009] S2, Preparation of antibacterial gel coating:

[0010] S2.1, Preparation of alkenyl hyaluronic acid: Hyaluronic acid was uniformly dissolved in deionized water, and then 4-(ethylene oxide-2-ylmethoxy)butyl acrylate was added. The mixture was stirred at 50-60℃ for 20-24 h. Then, lysine solution was added at room temperature and the reaction was terminated by stirring for 30-50 min. After dialyzing in water for 3-4 days, the mixture was freeze-dried to obtain alkenyl hyaluronic acid.

[0011] S2.2, mix the alkenyl hyaluronic acid aqueous solution and the quercetin-3-O-glucoside methanol solution evenly, then add it to the TCS@ZIF-8 mixture, stir for 3-5 hours, add the photoinitiator, and obtain the antibacterial gel coating solution;

[0012] S3. Immerse the PDO mixed suture matrix in the antibacterial gel coating solution for 2-3 hours, remove it, vacuum dry it, and then irradiate it under ultraviolet light for 30-45 seconds. After cleaning and drying, cut out the barbs to obtain PDO barbed sutures with antibacterial coating.

[0013] By adopting the above technical solution

[0014] Preferably, the ratio of L-polylactic acid to polydioxanone in S1 is (35-55)g:(45-65)g.

[0015] By adopting the above technical solution, a PDO mixed suture matrix with good mechanical properties is obtained by blending and spinning polylactic acid (PLA) and polydioxanone (PDX) in a certain proportion. Then, hyaluronic acid is used as a gel matrix and 4-(ethylene oxide-2-ylmethoxy)butyl acrylate is grafted onto hyaluronic acid for alkenyl modification. Then, it is mixed with quercetin-3-O-glucoside with antibacterial properties and TCS@ZIF-8 loaded with oridonin with antibacterial activity to obtain an antibacterial gel coating. Finally, the PDO mixed suture matrix is ​​impregnated in the antibacterial gel coating and irradiated with ultraviolet light under a photoinitiator to obtain a PDO barbed suture with excellent mechanical properties and durable antibacterial properties.

[0016] Preferably, in step S2.1, the ratio of hyaluronic acid, deionized water, 4-(ethylene oxide-2-ylmethoxy)butyl acrylate, and lysine solution is (5-10) g:(500-800) mL:(15-20) mL:(20-27) mL; and the concentration of the lysine solution is 20-25 wt%.

[0017] Hyaluronic acid, a naturally occurring polysaccharide, is widely distributed in various tissues and body fluids of the human body. It has a variety of active functional groups such as carboxyl, hydroxyl and amide groups. By reacting the carboxyl group with 4-(ethylene oxide-2-ylmethoxy)butyl acrylate through ring opening, a modified hydrogel with alkenyl functional groups is obtained, which prepares for the subsequent loading of antibacterial components.

[0018] Preferably, in step S2.2, the ratio of the amount of alkenyl hyaluronic acid aqueous solution, quercetin-3-O-glucoside methanol solution, TCS@ZIF-8 solution, and photoinitiator is (20-40) mL:(12-18) mL:(7-12) mL:(14-22) mg.

[0019] By adopting the above technical solution, on the one hand, quercetin-3-O-glucoside has multiple effects such as anti-oxidation, anti-inflammation, and antibacterial properties. When used in wound suturing, it is beneficial to wound healing and reduces postoperative infection. At the same time, quercetin-3-O-glucoside contains multiple hydrogen bonds and is stable under ultraviolet light, which is conducive to forming hydrogen bonds with the carbonyl group in alkenyl hyaluronic acid and is not destroyed by ultraviolet light.

[0020] Preferably, in step S2.2, the concentration of the alkenyl hyaluronic acid aqueous solution is 4.3-6.7 wt%, the concentration of the quercetin-3-O-glucoside methanol solution is 3.5-5.8 wt%, and the concentration of the TCS@ZIF-8 solution is 7.2-8.5 wt%.

[0021] Preferably, the photoinitiator is 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylphenylacetone.

[0022] By adopting the above technical solution, the alkenyl group undergoes a polymerization reaction under external light to form a chemical cross-linked network. At the same time, the hydrogen bonding between the hydroxyl group of quercetin-3-O-glucoside and the carbonyl group in the alkenyl hyaluronic acid is conducive to the formation of a physical cross-linked network. The double cross-linked network structure is conducive to the stable dispersion of TCS@ZIF-8.

[0023] Preferably, the preparation method of the TCS@ZIF-8 solution is as follows: (1) Dissolve 2-methylimidazolium and oridonin in methanol, then pour in zinc nitrate solution under stirring, stir for 2-3 min, then centrifuge at 6000-8000 r / min for 20-30 min, collect the product, and wash with methanol to obtain TCS@ZIF-8; (2) Dilute TCS@ZIF-8 with methanol to obtain a 7.2-8.5 wt% TCS@ZIF-8 mixture.

[0024] Preferably, the ratio of the amounts of 2-methylimidazole, oridonin, methanol, and zinc nitrate solution is (4.6-6.2)g:(80-100)mg:(120-180)mL:(75-92)mL; and the concentration of the nitric acid solution is 20-25mg / mL.

[0025] Preferably, the stirring speed in (1) is 650-850 r / min.

[0026] By adopting the above technical solution, and by preparing nano-sized TCS@ZIF-8 with regular morphology and good biocompatibility, oridonin is loaded into the zeolite imidazole ester framework, which avoids the destruction and inactivation of oridonin during ultraviolet cross-linking, and at the same time enables the slow release of oridonin, thereby enhancing its antibacterial durability.

[0027] Secondly, this application provides a PDO barbed suture with an antibacterial coating, which is prepared using the above-described method.

[0028] In summary, this application has the following beneficial effects:

[0029] 1. In this application, polylactic acid (PLA) and polydioxanone (PDX) are blended and spun in a certain proportion to obtain a PDO mixed suture matrix with good mechanical properties. Then, hyaluronic acid is used as a gel matrix and 4-(ethylene oxide-2-ylmethoxy)butyl acrylate is grafted onto the hyaluronic acid for alkenyl modification. Then, it is mixed with quercetin-3-O-glucoside, which has antibacterial properties, and TCS@ZIF-8 loaded with oridonin, which has antibacterial activity, to obtain an antibacterial gel coating. Finally, the PDO mixed suture matrix is ​​impregnated in the antibacterial gel coating and irradiated with ultraviolet light under a photoinitiator to obtain a PDO barbed suture with excellent mechanical properties and durable antibacterial properties.

[0030] 2. This application preferentially uses 2-methylimidazole, zinc nitrate, and oridonin to prepare nano-TCS@ZIF-8 particles with strong sustained-release properties, biocompatibility, thermal stability, and water stability. These particles are then co-added with quercetin-3-O-glucoside to an alkenylated gel coating. Photo-initiated alkenyl polymerization and the synergistic hydrogen bonding between multiple hydroxyl groups in quercetin-3-O-glucoside and the carbonyl groups in alkenyl hyaluronic acid form a chemical-physical dual network structure, enabling the TCS@ZIF-8 particles to be stably dispersed within it. The gel coating fills the gaps in the braided PDO mixed suture matrix, forming a smooth surface and reducing tissue damage caused by the sutures.

[0031] 3. The PDO barbed suture with antibacterial coating prepared by the method of this application has the characteristics of smooth coating, moderate thickness, high and long-lasting antibacterial performance, high strength, good flexibility, low friction, and strong operability. Attached Figure Description

[0032] Figure 1 The reaction mechanism of the antibacterial gel coating in this application.

[0033] Figure 2 Scanning electron microscope image of TCS@ZIF-8 prepared in Example 3 of this application.

[0034] Figure 3 The scanning electron microscope image of the PDO barbed suture with antibacterial coating prepared in Example 3 of this application, wherein a is the PDO mixed suture matrix, and b, c, and d are PDO barbed sutures with antibacterial coating before the barbs are cut at different magnifications.

[0035] Figure 4 Example 3 of this application shows the antibacterial test diagram of the PDO barbed suture with antibacterial coating prepared in this study. Detailed Implementation

[0036] The present application will be further described in detail below with reference to the embodiments.

[0037] Unless otherwise specified, the raw materials used in the embodiments and comparative examples of this application are all commercially available.

[0038] Example

[0039] Example 1

[0040] A method for preparing PDO barbed sutures with an antibacterial coating, characterized by comprising the following steps:

[0041] S1, Preparation of PDO hybrid suture matrix: 35g of L-polylactic acid and 65g of polydioxanone were thoroughly dried and mixed, melted and spun at 150℃ for 20min, and then spun and stretched to obtain monofilaments. Then, 8 monofilaments were used as shell yarns and 2 identical monofilaments were used as core yarns. Using a domestic 8-spindle vertical medical braiding machine, a No. 0 braided suture, i.e., PDO hybrid suture matrix, was prepared.

[0042] S2, Preparation of antibacterial gel coating:

[0043] S2.1, Preparation of alkenyl hyaluronic acid: Dissolve 5g of hyaluronic acid uniformly in 500mL of deionized water, then add 15mL of 4-(ethylene oxide-2-ylmethoxy)butyl acrylate, stir at 50℃ for 24h, then add 20mL of 25wt% lysine solution at room temperature, stir for 30min to terminate the reaction, dialyze in water for 3 days and freeze dry to obtain alkenyl hyaluronic acid;

[0044] S2.2, 20 mL of 6.7 wt% alkenyl hyaluronic acid aqueous solution and 12 mL of 5.8 wt% quercetin-3-O-glucoside methanol solution were mixed evenly, and then 7 mL of 8.5 wt% TCS@ZIF-8 mixture was added. The mixture was stirred for 3 h, and 14 mg of photoinitiator 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylphenylacetone was added to obtain antibacterial gel coating solution;

[0045] S3, immerse the PDO-mixed suture matrix in an antibacterial gel coating solution for 2 hours, remove it, vacuum dry it, then irradiate it under ultraviolet light for 30 seconds, clean and dry it, and cut out barbs to obtain PDO barbed sutures with an antibacterial coating; the wavelength of the ultraviolet light is 450nm, and the light intensity is 7W / cm². 2 .

[0046] The preparation method of the TCS@ZIF-8 solution is as follows: (1) Dissolve 4.6g of 2-methylimidazolium and 80mg of oridonin in 120mL of methanol, then pour in 75mL of zinc nitrate solution with a concentration of 20mg / mL under stirring at 650r / min, continue stirring for 2min, then centrifuge at 6000r / min for 30min, collect the product, and wash with methanol to obtain TCS@ZIF-8; (2) Dilute TCS@ZIF-8 with methanol to prepare a TCS@ZIF-8 mixture with a concentration of 8.5wt%.

[0047] Example 2

[0048] A method for preparing PDO barbed sutures with an antibacterial coating, characterized by comprising the following steps:

[0049] S1, Preparation of PDO hybrid suture matrix: 45g of L-polylactic acid and 55g of polydioxanone were thoroughly dried and mixed, melted and spun at 160℃ for 15min, and then spun and stretched to obtain monofilaments. Then, 8 monofilaments were used as shell yarns and 2 identical monofilaments were used as core yarns. Using a domestic 8-spindle vertical medical braiding machine, a No. 0 braided suture, i.e., PDO hybrid suture matrix, was prepared.

[0050] S2, Preparation of antibacterial gel coating:

[0051] S2.1, Preparation of alkenyl hyaluronic acid: Dissolve 8g of hyaluronic acid uniformly in 650mL of deionized water, then add 18mL of 4-(ethylene oxide-2-ylmethoxy)butyl acrylate, stir at 55℃ for 22h, then add 27mL of 20wt% lysine solution at room temperature, stir for 40min to terminate the reaction, dialyze in water for 3 days and freeze dry to obtain alkenyl hyaluronic acid;

[0052] S2.2, 40 mL of 4.3 wt% alkenyl hyaluronic acid aqueous solution and 18 mL of 3.5 wt% quercetin-3-O-glucoside methanol solution were mixed evenly, and then added to 12 mL of 7.2 wt% TCS@ZIF-8 mixture. The mixture was stirred for 4 h, and then 19 mg of photoinitiator 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylphenylacetone was added to obtain the antibacterial gel coating solution.

[0053] S3, the PDO-mixed suture matrix is ​​immersed in an antibacterial gel coating solution for 2.5 hours, removed, vacuum dried, and then irradiated under ultraviolet light for 40 seconds. After cleaning and drying, barbs are cut out to obtain PDO barbed sutures with an antibacterial coating; the wavelength of the ultraviolet light is 450 nm, and the light intensity is 7 W / cm². 2 .

[0054] The preparation method of the TCS@ZIF-8 solution is as follows: (1) Dissolve 5.4 g of 2-methylimidazole and 90 mg of oridonin in 150 mL of methanol, then pour in 83 mL of zinc nitrate solution with a concentration of 22 mg / mL under stirring at 750 r / min, continue stirring for 3 min, then centrifuge at 7000 r / min for 25 min, collect the product, and wash with methanol to obtain TCS@ZIF-8; (2) Dilute TCS@ZIF-8 with methanol to prepare a TCS@ZIF-8 mixture with a concentration of 7.2 wt%.

[0055] Example 3

[0056] A method for preparing PDO barbed sutures with an antibacterial coating, characterized by comprising the following steps:

[0057] S1, Preparation of PDO hybrid suture matrix: 55g of L-polylactic acid and 45g of polydioxanone were thoroughly dried and mixed, melted and spun at 170℃ for 10min, and then spun and stretched to obtain monofilaments. Then, 8 monofilaments were used as shell yarns and 2 identical monofilaments were used as core yarns. Using a domestic 8-spindle vertical medical braiding machine, a No. 0 braided suture, i.e., PDO hybrid suture matrix, was prepared.

[0058] S2, Preparation of antibacterial gel coating:

[0059] S2.1, Preparation of alkenyl hyaluronic acid: 10g of hyaluronic acid was uniformly dissolved in 800mL of deionized water, and then 20mL of 4-(ethylene oxide-2-ylmethoxy)butyl acrylate was added. The mixture was stirred at 60℃ for 20h. Then, 24mL of 23wt% lysine solution was added at room temperature and the reaction was terminated by stirring for 50min. After dialyzing in water for 4 days, the mixture was freeze-dried to obtain alkenyl hyaluronic acid.

[0060] S2.2, 30 mL of 5.4 wt% alkenyl hyaluronic acid aqueous solution and 15 mL of 4.2 wt% quercetin-3-O-glucoside methanol solution were mixed evenly, and then added to 10 mL of 7.8 wt% TCS@ZIF-8 mixture. The mixture was stirred for 5 h, and then 22 mg of photoinitiator 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylphenylacetone was added to obtain antibacterial gel coating solution;

[0061] S3, immerse the PDO-mixed suture matrix in an antibacterial gel coating solution for 3 hours, remove it, vacuum dry it, then irradiate it under ultraviolet light for 45 seconds, clean and dry it, and cut out barbs to obtain PDO barbed sutures with an antibacterial coating; the wavelength of the ultraviolet light is 450nm, and the light intensity is 7W / cm². 2 .

[0062] The preparation method of the TCS@ZIF-8 solution is as follows: (1) Dissolve 6.2g of 2-methylimidazolium and 100mg of oridonin in 180mL of methanol, then pour in 92mL of zinc nitrate solution with a concentration of 25mg / mL under stirring at 850r / min, continue stirring for 3min, then centrifuge at 8000r / min for 30min, collect the product, and wash with methanol to obtain TCS@ZIF-8; (2) Dilute TCS@ZIF-8 with methanol to prepare a TCS@ZIF-8 mixture with a concentration of 7.8wt%.

[0063] The microstructure of the prepared TCS@ZIF-8 was observed using scanning electron microscopy, and the results are as follows: Figure 2 As shown. From Figure 2As can be seen, TCS@ZIF-8 has a regular crystal structure with uniform particle size of approximately 160 nm.

[0064] Comparative Example 1

[0065] Similar to Example 3, except that the amount of polylactic acid (PLA) 55g and polydioxanone (PD-dioxanone) 45g added in S1 was adjusted to PLA 60g and PD-dioxanone 40g.

[0066] Comparative Example 2

[0067] Similar to Example 3, except that in S2.2, hyaluronic acid is directly replaced with alkenyl hyaluronic acid.

[0068] Comparative Example 3

[0069] Similar to Example 3, except that in S2.2, an equimolar amount of oridonin A is used instead of TCS@ZIF-8.

[0070] Comparative Example 4

[0071] Similar to Example 3, except that the concentration of the TCS@ZIF-8 mixture in S2.2 was adjusted to 10.0 wt%. Comparative Example 5

[0072] Same as Example 3, except that S2.2 does not contain quercetin-3-O-glucoside methanol solution.

[0073] Performance testing

[0074] Morphological observation: The micromorphological characteristics of the PDO hybrid suture matrix prepared in Example 3 and the PDO barbed suture with antibacterial coating before the barbs were cut were observed using scanning electron microscopy. The results are as follows: Figure 3 As shown. From Figure 3 As shown in (a), the PDO hybrid suture matrix has a regular structure with a surface of regular woven gaps. In (b), before the barbs are cut, the PDO hybrid suture with an antibacterial coating shows that the antibacterial gel coating evenly covers the PDO hybrid suture matrix and fills the woven gaps, resulting in a smooth surface. The thickness of the antibacterial gel coating is approximately 0.04 mm. In (c), the antibacterial gel coating exhibits a uniformly distributed porous structure. In (d), the pore size is approximately 3.5 μm. The large number of pores on the surface of the antibacterial gel coating indicates its strong water storage capacity. The water content promotes the transfer of water vapor and oxygen, which is beneficial for cell adhesion and growth, promotes cell metabolism, and accelerates wound repair.

[0075] Mechanical property testing: Samples were taken from the PDO barbed sutures with antibacterial coating prepared in Examples 1-3 and Comparative Examples 1-5, as well as the PDO mixed suture matrix prepared in Example 3. Their breaking strength and elongation at break were tested on a yarn tensile testing machine with a tensile rate of 100 mm / min. Their bending stiffness was tested using the cantilever beam method. Their dynamic friction was tested using a suture friction performance tester.

[0076] Antibacterial durability test: The antibacterial properties of PDO barbed sutures with antibacterial coatings prepared in Examples 1-3 and Comparative Examples 1-5, as well as the PDO mixed suture matrix prepared in Example 3, against Staphylococcus aureus and Escherichia coli were tested using the agar plate diffusion method. The sutures were transferred to a new petri dish every 24 hours until the inhibition zone disappeared and bacteria multiplied under the sutures. The time was recorded and one day was subtracted to represent the antibacterial time.

[0077] The results are shown in Table 1 and Figure 4 As shown;

[0078] Table 1 Performance Test Results

[0079]

[0080] As can be seen from Table 1, the PDO barbed sutures with antibacterial coating prepared in Examples 1-3 of this application have excellent mechanical properties and antibacterial durability. Based on the PDO mixed suture matrix prepared in Comparative Example 1 and Example 3, it can be seen that the suture prepared using the addition ratio of polylactic acid to polydioxanone described in this application has both strength and flexibility. Based on Comparative Examples 2, 3, and 5, it can be seen that the addition of alkenyl-functionalized hyaluronic acid, oridonin TCS@ZIF-8, and quercetin-3-O-glucoside all help to improve the antibacterial durability of the antibacterial coated PDO barbed suture, and have little impact on its mechanical properties. Based on the PDO mixed suture matrix prepared in Example 3, it can be seen that the PDO barbed suture with antibacterial coating prepared in this application has a small dynamic friction force, which means that when suturing a wound, it causes less dragging damage to the contact tissue. Based on Comparative Example 4, it can be seen that when the concentration of TCS@ZIF-8 mixture is too high, it will increase the dynamic friction force of the PDO barbed suture with antibacterial coating.

[0081] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, and such modifications are protected by patent law as long as they fall within the scope of protection claimed in this application.

Claims

1. A method for preparing PDO barbed sutures with an antibacterial coating, characterized in that, Includes the following steps: S1, Preparation of PDO mixed suture matrix: Polylactic acid (PLA) and polydioxanone (PD-dioxanone) are mixed in proportion, melted and mixed at 150-170℃ for 10-20 min, spun and stretched, and woven with a braiding machine to obtain PDO mixed suture matrix; S2, Preparation of antibacterial gel coating: S2.1, Preparation of alkenyl hyaluronic acid: Hyaluronic acid was uniformly dissolved in deionized water, and then 4-(ethylene oxide-2-ylmethoxy)butyl acrylate was added. The mixture was stirred at 50-60℃ for 20-24 h. Then, lysine solution was added at room temperature and the reaction was terminated by stirring for 30-50 min. After dialyzing in water for 3-4 days, the mixture was freeze-dried to obtain alkenyl hyaluronic acid. S2.2, mix the alkenyl hyaluronic acid aqueous solution and the quercetin-3-O-glucoside methanol solution evenly, then add it to the TCS@ZIF-8 mixture, stir for 3-5 hours, add the photoinitiator, and obtain the antibacterial gel coating solution; S3, Immerse the PDO mixed suture matrix in the antibacterial gel coating solution for 2-3 hours, take it out, vacuum dry it, and then irradiate it under ultraviolet light for 30-45 seconds. After cleaning and drying, cut out the barbs to obtain PDO barbed sutures with antibacterial coating. The ratio of L-polylactic acid to polydioxanone in S1 is (35-55) g: (45-65) g; The preparation method of the TCS@ZIF-8 solution is as follows: (1) Dissolve 2-methylimidazolium and oridonin in methanol, then pour in zinc nitrate solution under stirring, stir for 2-3 min, then centrifuge at 6000-8000 r / min for 20-30 min, collect the product, and wash with methanol to obtain TCS@ZIF-8; (2) Dilute TCS@ZIF-8 with methanol to obtain a 7.2-8.5 wt% TCS@ZIF-8 mixture.

2. The method for preparing PDO barbed sutures with an antibacterial coating according to claim 1, characterized in that, In S2.1, the ratio of hyaluronic acid, deionized water, 4-(ethylene oxide-2-ylmethoxy)butyl acrylate, and lysine solution is (5-10) g: (500-800) mL: (15-20) mL: (20-27) mL; the concentration of the lysine solution is 20-25 wt%.

3. The method for preparing PDO barbed sutures with an antibacterial coating according to claim 2, characterized in that, In S2.2, the ratio of the amount of alkenyl hyaluronic acid aqueous solution, quercetin-3-O-glucoside methanol solution, TCS@ZIF-8 solution, and photoinitiator is (20-40) mL: (12-18) mL: (7-12) mL: (14-22) mg.

4. The method for preparing PDO barbed sutures with an antibacterial coating according to claim 3, characterized in that, In step S2.2, the concentration of the alkenyl hyaluronic acid aqueous solution is 4.3-6.7 wt%, the concentration of the quercetin-3-O-glucoside methanol solution is 3.5-5.8 wt%, and the concentration of the TCS@ZIF-8 solution is 7.2-8.5 wt%.

5. The method for preparing PDO barbed sutures with an antibacterial coating according to claim 4, characterized in that, The photoinitiator is 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylphenylacetone.

6. The method for preparing PDO barbed sutures with an antibacterial coating according to claim 5, characterized in that, The ratio of the amounts of 2-methylimidazole, oridonin, methanol, and zinc nitrate solution is (4.6-6.2) g: (80-100) mg: (120-180) mL: (75-92) mL; the concentration of the nitric acid solution is 20-25 mg / mL.

7. The method for preparing PDO barbed sutures with an antibacterial coating according to claim 6, characterized in that, The stirring speed in (1) is 650-850 r / min.

8. A PDO barbed suture with an antibacterial coating, characterized in that, It is obtained by the preparation method of PDO barbed suture with antibacterial coating as described in any one of claims 1-7.

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