Application of rutin in improving germination rate of tobacco seeds under cadmium stress

Abstract

The invention discloses application of rutin in improving the germination rate of tobacco seeds under cadmium stress, and belongs to the technical field of planting. The method for improving the germination rate of the tobacco seeds under the cadmium stress comprises the step of applying rutin before the tobacco seeds germinate. According to the method disclosed by the invention, by exogenously adding rutin, the germination rate of the tobacco seeds under the stress of high-concentration heavy metal cadmium is remarkably improved, and the method plays an important role in remediation of a heavy metal polluted environment.

Description

Technical Field

[0001] The invention relates to the field of planting technology, in particular to application of rutin in improving the germination rate of tobacco seeds under cadmium stress. Background Art

[0002] Cadmium is a common heavy metal pollutant and one of the heavy metals with the highest soil enrichment content. After entering the soil, it not only affects the physical and chemical properties of the soil, but also affects the growth and development of plants, seriously affecting the development of modern agriculture and green economic industries. The heavy metal cadmium is absorbed by plants and enriched in the plant body, and eventually enters the human body through the food chain and accumulates, seriously endangering human health. Studies have shown that under heavy metal stress, the stomata of plant leaves are closed, resulting in slower photosynthesis and transpiration of plants. If the heavy metal content in the soil exceeds the tolerance level of the plant, it will affect the development of plant roots and leaves. Plants have different tolerance levels to heavy metals at different growth stages. The impact of heavy metals on seed germination also affects the growth of the entire plant to a certain extent. In the process of plant growth, the seed germination period is the most vulnerable and is most affected by the outside world.

[0003] At present, spermidine (Spd), nano-titanium dioxide (TiO2-NPs), copper oxide nanoparticles, etc. are mainly used as seed soaking materials, which can reduce plant Cd absorption and effectively improve the germination vitality of seeds under Cd stress. However, the cost of nanomaterials is too high and is not suitable for large-scale production and application. Therefore, it is urgent to find a new solution. Summary of the invention

[0004] The purpose of the present invention is to provide an application of rutin in improving the germination rate of tobacco seeds under cadmium stress, so as to solve the problems existing in the above-mentioned prior art.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] One of the technical solutions of the present invention is a method for improving the germination rate of tobacco seeds under cadmium stress, which comprises applying rutin before the tobacco seeds germinate.

[0007] Furthermore, when the cadmium concentration under cadmium stress is 300 mg / L, the application concentration of rutin is 50 μM.

[0008] The second technical solution of the present invention: application of rutin in improving the germination rate of tobacco seeds under cadmium stress.

[0009] Furthermore, when the cadmium concentration under cadmium stress is 300 mg / L, the application concentration of rutin is 50 μM.

[0010] Rutin is an important product of flavonoid metabolism, with very strong antioxidant capacity and biological activity. The application of rutin can reduce the accumulation of peroxides in seeds caused by heavy metal stress and improve the seed germination rate. At the same time, rutin can promote the formation of complexes with heavy metals, fix or degrade heavy metals, reduce the concentration of heavy metals in seed cells, and thus improve the seed germination rate. After the application of rutin, cadmium ions will be enriched in the cell wall, and the cadmium content in the cell vacuole will be reduced, thereby improving the tolerance of the plant.

[0011] The present invention discloses the following technical effects:

[0012] The present invention significantly improves the germination rate of tobacco seeds under high-concentration heavy metal cadmium stress by exogenously adding rutin, and plays an important role in the restoration of heavy metal polluted environments. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0014] Figure 1 This is a statistical result diagram of the germination rate of tobacco seeds treated with different cadmium nitrate concentrations in Example 1;

[0015] Figure 2 The figure is a statistical result diagram of the inhibitory effect of different cadmium nitrate concentrations on the germination rate of tobacco seeds in Example 1;

[0016] Figure 3 This is the result of the effect of exogenous addition of rutin on the germination rate of tobacco seeds under cadmium nitrate treatment. DETAILED DESCRIPTION

[0017] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0018] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0019] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0020] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to the skilled artisan. The present invention description and examples are exemplary only.

[0021] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0022] The tobacco seed variety used in the specific embodiment of the present invention is Zhongyan 300.

[0023] Example 1

[0024] Application of rutin in improving tobacco seed germination rate under cadmium stress:

[0025] (I) Effect of cadmium nitrate on tobacco seed germination rate

[0026] (1) Seed disinfection

[0027] Place tobacco seeds in a 2mL centrifuge tube, add 1mL of 75% by volume ethanol and soak for 30 seconds, absorb the ethanol, wash with sterile water three times, add 1mL of 2% by volume sodium hypochlorite and soak for 5 minutes, absorb the sodium hypochlorite, then wash with sterile water three times, absorb the water with filter paper to obtain sterilized tobacco seeds.

[0028] (2) Seeding and germination rate statistics

[0029] Two layers of filter paper were laid in a 9 cm diameter round dish, 3 mL of cadmium nitrate solution of different concentrations (100 mg / L (Cd-100), 200 mg / L (Cd-200) or 300 mg / L (Cd-300)) was dripped onto the filter paper, and clean water without cadmium nitrate was used as a blank control (CK). After the filter paper was fully moistened with water, the sterilized tobacco seeds were evenly planted on the filter paper of the culture dish using a toothpick, 50 seeds were planted per dish, and repeated 3 times. After the planting was completed, the seeds were covered to keep moist, and placed at room temperature. After 5 days, the germination rate was counted and calculated, and the statistics were counted once a day for 7 days. The results are shown in Table 1. Figure 1 and Figure 2 .

[0030] from Figure 1 and Figure 2 It can be seen that cadmium nitrate has an important effect on the germination rate of tobacco seeds. Different concentrations of cadmium nitrate significantly reduce the germination rate of tobacco seeds, and the inhibitory effect increases with the increase of concentration; the inhibitory effect of cadmium nitrate solution on tobacco seed germination is most significant in the early stage of seed germination, that is, 5 to 6 days after sowing. The experimental statistical results show that on the 5th day after sowing, the germination rate of tobacco seeds treated with 100mg / L, 200mg / L and 300mg / L cadmium nitrate was reduced by 31.3%, 87.88% and 100% respectively compared with the clear water control ((germination rate of clear water control-germination rate of treatment group) / germination rate of clear water control × 100%); on the 6th day after sowing, the germination rate of tobacco seeds treated with 100mg / L, 200mg / L and 300mg / L cadmium nitrate was reduced by 0.71%, 21.42% and 50% respectively compared with the clear water control.

[0031] (II) Effect of rutin on germination rate of tobacco seeds under cadmium nitrate treatment

[0032] Adding rutin exogenously to a 300 mg / L cadmium nitrate solution, so that the concentration of rutin in the cadmium nitrate solution is 0 μM, 50 μM, 100 μM, 150 μM, and 200 μM, respectively, to obtain cadmium nitrate solutions containing rutin at different concentrations;

[0033] Two layers of filter paper were laid in a round dish with a diameter of 9 cm. 3 mL of cadmium nitrate solution containing different concentrations of rutin was dripped onto the filter paper. Clean water without cadmium nitrate and rutin was used as a blank control (CK). After the filter paper was fully moistened with water, the sterilized tobacco seeds were evenly planted on the filter paper of the culture dish using a toothpick. 50 seeds were planted in each dish. This was repeated 3 times. After the planting was completed, the dish was covered to keep it moist. The dish was placed at room temperature. After 3 days, the germination rate was counted and calculated. The germination rate was counted once a day. The results are shown in Table 1. Figure 3 .

[0034] Figure 3 CK in the table is a blank control with 3 mL of water added;

[0035] Cd-300 is the treatment group with 3 mL of 300 mg / L cadmium nitrate solution added;

[0036] RT-50 is the treatment group to which 3 mL of cadmium nitrate solution containing rutin (rutin concentration is 50 μM, cadmium nitrate concentration is 300 mg / L) was added;

[0037] RT-100 is a treatment group to which 3 mL of cadmium nitrate solution containing rutin (rutin concentration is 100 μM, cadmium nitrate concentration is 300 mg / L) was added;

[0038] RT-150 is a treatment group to which 3 mL of cadmium nitrate solution containing rutin (rutin concentration is 150 μM, cadmium nitrate concentration is 300 mg / L) was added;

[0039] RT-200 is a treatment group to which 3 mL of cadmium nitrate solution containing rutin (rutin concentration is 200 μM, cadmium nitrate concentration is 300 mg / L) was added.

[0040] from Figure 3 It can be seen that compared with the control treated with clear water, the 300 mg / L cadmium nitrate treatment significantly reduced the germination rate of tobacco seeds. Starting from the 5th day after sowing, the germination rate of tobacco seeds treated with cadmium nitrate solution with 50 μM rutin (rutin concentration of 50 μM, cadmium nitrate concentration of 300 mg / L) was significantly higher than that treated with cadmium nitrate without rutin.

[0041] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for improving the germination rate of tobacco seeds under cadmium stress, characterized in that: It involves applying rutin to tobacco seeds before germination.

2. The method for improving tobacco seed germination rate under cadmium stress according to claim 1, characterized in that: When the cadmium concentration under cadmium stress is 300 mg / L, the application concentration of rutin is 50 μM.

3. Application of rutin in improving the germination rate of tobacco seeds under cadmium stress.

4. The use according to claim 3, characterized in that: When the cadmium concentration under cadmium stress is 300 mg / L, the application concentration of rutin is 50 μM.

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