Microbial agent and application thereof

Abstract

The invention provides a microbial agent and application thereof, and belongs to the technical field of biology, the microbial agent comprises the following components: a component A: Penicillium restrictum and Penicillium verruculosum, a component B: Penicillium verruculosum, a component C: Penicillium verruculosum, and a component D: Penicillium verruculosum; the component B comprises sodium alginate, fulvic acid, a panax japonicus extract, a polygonatum odoratum extract and corn starch; wherein the addition amount of the penicillium limited accounts for 1%-3% of the total weight of the microbial agent, the addition amount of the penicillium verrucosum accounts for 1%-5% of the total weight of the microbial agent, and the balance is the component B. The microbial agent provided by the invention can be used for effectively preventing and treating the Chinese cabbage soft rot caused by the pectobacterium carotovorum (Pectobacterium carotovorum). The microbial agent provided by the invention can be used for effectively preventing and treating the Chinese cabbage soft rot caused by the pectobacterium carotovorum.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and specifically relates to a microbial agent and application thereof. Background Art

[0002] Chinese cabbage( Turnip cabbage ssp .of Beijing ) is a vegetable of the genus Brassica in the family Cruciferae. It is a leafy vegetable that is widely grown in my country and prefers a warm and humid environment.

[0003] Pectinobacterium carotovora Peclobacteriumcarolovorumsubsp.carolovorum ) is a common pathogen of cabbage soft rot. The pathogen can invade the seedlings or root seedlings of the plant and cause disease. After the occurrence of soft rot, the cabbage plants will rot and deteriorate, seriously affecting the yield of cabbage and restricting the development of cabbage planting. At present, soft rot is mostly controlled by chemical drugs. The long-term application of traditional chemical agents has increasingly prominent harm to the soil ecological environment, agricultural product safety and human and animal health. Biological control has the advantages of being green, safe, non-toxic and harmless, and meets the requirements of sustainable agricultural development. It is an important direction and research hotspot for the prevention and control of vegetable soft rot and has broad application prospects. Summary of the invention

[0004] In order to overcome the problems existing in the prior art, the present invention provides a microbial agent and application thereof.

[0005] To achieve the above object, the present invention provides a microbial agent in a first aspect, comprising the following components: Component A: Penicillium restrictum ( Penicillium restricted ) and Penicillium verrucosum ( Penicillium verruculosum ); Component B: sodium alginate, fulvic acid, ginseng extract, polygonatum odoratum extract, corn starch; Among them, the addition amount of Penicillium restrictum is 1%-3% of the total weight of the microbial agent, the addition amount of Penicillium verrucosus is 1%-5% of the total weight of the microbial agent, and the remainder is component B.

[0006] Furthermore, the effective viable count of Penicillium restrictum and Penicillium verrucosum in component A is 1×10 9 -2x10 9 CFU / g.

[0007] Furthermore, the mass ratio of sodium alginate, fulvic acid, Panax notoginseng extract, Polygonatum odoratum extract and corn starch in component B is 6-8:90-120:2-3:1-4:6-9.

[0008] The second aspect of the present invention provides the use of the microbial agent of the first aspect in preventing and treating soft rot of cabbage, wherein the pathogen of soft rot is Pectinobacterium carotovora ( Peclobacteriumcarolovorumsubsp.carolo we were ).

[0009] The third aspect of the present invention provides a method for preventing and controlling soft rot of cabbage, the method comprising applying the microbial agent described in the first aspect to the rhizosphere of cabbage seedlings.

[0010] Through the above technical scheme, the present invention can at least achieve the following beneficial effects: the microbial agent provided by the present invention can effectively prevent and control carrot soft rot pectin bacteria ( Peclobacteriumcarolovorumsubsp.carolovorum ) caused by soft rot of cabbage. DETAILED DESCRIPTION

[0011] Unless otherwise specified, the materials and reagents used in the present invention are commercially available.

[0012] Embodiment 1 In the present invention, Penicillium restrictedum and Penicillium verrucosum were purchased from Ningbo Mingzhou Biotechnology Co., Ltd. The product number of Penicillium restrictedum is BMZ072236, and the product number of Penicillium verrucosum is BMZ074093. After activation and expansion culture, the effective viable count of Penicillium restrictedum was about 1.5x10 10 CFU / g, the effective viable count of Penicillium verrucosum is about 2x10 10 CFU / g; The sodium alginate in the present invention was purchased from Shandong Huiheng Biotechnology Co., Ltd. Fulvic acid CAS No. 479-66-3 in the present invention was purchased from Wuhan Huaxiang Kejie Biotechnology Co., Ltd. The Panax notoginseng extract and Polygonatum odoratum extract in the present invention were purchased from Shanyang Lianfeng Biotechnology Co., Ltd., and the extracts were all water extracts with a specification of 10:1; Corn starch is a common commercial product.

[0013] Prepare the microbial agent according to Table 1 below: Table 1 Microbial agent formula There is no particular restriction on the preparation method of the microbial agent. Any method of adding biocontrol bacteria to the composition in the art can be applied to the present invention. For example, Penicillium restrictum and Penicillium verrucosum can be first made into a microbial agent, and then directly added to the aforementioned ingredients according to the proportions in the above table, or the Penicillium restrictum and Penicillium verrucosum can be packaged separately from the aforementioned ingredients, and then mixed or applied together before use. For another example, Penicillium restrictum and Penicillium verrucosum can be expanded, and the obtained culture solution can be used to prepare the aforementioned microbial agent according to a certain ratio. Taking into account the convenience of the preparation process and use, it is preferred to use Penicillium restrictum and Penicillium verrucosum and add them to the aforementioned formula.

[0014] Embodiment 2 Select three true-leaf Chinese cabbage seedlings with good growth and basically the same size, and use a concentration of 1×10 8 The cabbage was irrigated with a suspension of Pectinobacterium carotovora at a concentration of CFU / mL, with 200 mL per plant. Seven days after irrigating the roots, the cabbage plants were irrigated with a microbial agent prepared according to the formula in Table 1, 30 g per plant; 50 replicates were set for each formula, and the disease index was investigated 14 days after the application of the microbial agent. The results are shown in Table 2 below.

[0015] According to the disease grading standards in the "Guidelines for Field Efficacy Tests of Pesticides", the disease is graded according to the proportion of the lesion area to the leaf area: Level 0 is 0; Level 1 is 1% to 5%; Level 3 is 6% to 10%; Level 5 is 11% to 25%; Level 7 is 26% to 50%; Level 9 is above 50%.

[0016] Disease index = Σ (number of diseased leaves at each level × disease level) / (total number of leaves investigated × highest disease level) × 100; Table 2 Disease index table It can be seen from Table 2 above that the microbial agent of the present invention can effectively prevent and treat the soft rot of cabbage caused by Pectinobacterium carotovora, and reduce the disease index of the soft rot of cabbage caused by Pectinobacterium carotovora.

[0017] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the present invention.

Claims

1. A microbial agent, characterized in that: Contains the following components: Component A: Penicillium restrictum ( Penicillium restrictum ) and Penicillium verrucosum ( Penicillium verruculosum ); Component B: sodium alginate, fulvic acid, ginseng extract, polygonatum odoratum extract, corn starch; Among them, the addition amount of Penicillium restrictum is 1%-3% of the total weight of the microbial agent, the addition amount of Penicillium verrucosus is 1%-5% of the total weight of the microbial agent, and the remainder is component B.

2. The microbial agent according to claim 1, characterized in that: The effective viable count of Penicillium restrictum and Penicillium verrucosum in component A is 1×10 9 -2x10 9 CFU / g.

3. A microbial agent according to claim 1, characterized in that: The mass ratio of sodium alginate, fulvic acid, Panax notoginseng extract, Polygonatum odoratum extract and corn starch in component B is 6-8:90-120:2-3:1-4:6-9.

4. Use of the microbial agent according to any one of claims 1 to 3 in preventing and treating soft rot of Chinese cabbage, wherein: The pathogen of the soft rot is Pectinobacterium carotovora Peclobacterium carolovorumsubsp.carolovorum ).

5. A method for preventing and treating soft rot of Chinese cabbage, characterized in that: The microbial agent according to any one of claims 1 to 3 is applied to the rhizosphere of cabbage seedlings.

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