Protease preparation for preventing pet hair ball disease as well as preparation method and application thereof

Abstract

The invention discloses a protease preparation for preventing pet hair ball disease as well as a preparation method and application thereof, and belongs to the technical field of pet nutrition and health. The protease preparation for preventing the pet hair ball disease is mainly prepared from a compound enzyme, vitamins, trace elements, dietary fibers, probiotics, meat powder and a wall material, and the mass ratio of the compound enzyme to the vitamins to the trace elements to the dietary fibers to the probiotics to the meat powder to the wall material is (1-100): (0.1-1): (0.1-10): (1-70): (0.1-10): (10-60): (10-60). The protease preparation provided by the invention can effectively degrade hair and indigestible food, prevents the hair and food residues from forming pellets to block gastrointestinal tracts, prevents hair bulb syndrome from occurring in mechanism, and is simple in preparation process and wide in application prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of animal nutrition and health, and specifically relates to a protease preparation for preventing pet hairball disease, and a preparation method and application thereof. Background Art

[0003] Unlike other pets, cats lick their fur to comb their hair, and the barbs on their tongues will bring the hair into the body. These indigestible hairs will be accumulated in the pylorus, the upper small intestine, and even the entire digestive tract along with undigested food. A small amount of hair accumulated in the stomach can be discharged through vomiting, but it will cause damage to the digestive tract. In severe cases, a large amount of hair will accumulate in the pylorus and the upper small intestine, making it impossible to vomit or swallow, which will lead to loss of appetite and even hunger strike. At this time, the hairballs must be removed surgically.

[0004] How to gently remove the hair accumulated in the gastrointestinal tract has always been a key concern for pet owners. Existing methods for removing hairballs include combing hair, feeding cat grass or hairball remover. Combing hair can certainly reduce the occurrence of hairball disease, but it requires pet owners to spend a lot of time and energy. Cat grass and hairball remover have two functions: 1. By stimulating the animal's stomach, vomiting out hairballs; 2. Intake of a large amount of oil and soluble fiber causes diarrhea / pseudo-diarrhea, promoting intestinal peristalsis to remove hair. However, these methods will cause damage to the front / back digestive tract and malnutrition, and long-term use is not good for pet health.

[0005] Hair is mainly composed of keratin, which is a protein that cannot be degraded by animal digestive enzymes. However, some microbial enzymes (such as keratinase) can degrade this type of protein to generate short peptides or amino acids. Existing keratinase is mostly used in textiles, food processing, and feed processing, but is less used in the field of alleviating hairball disease. Chinese patent CN114223788A discloses "a composite enzyme preparation and its application for effectively removing hairballs in pets". It uses composite keratinase, bromelain, papain and other enzyme preparations and other stabilizers to dissolve hairballs from the body, effectively preventing or reducing the occurrence of hairball diseases such as vomiting, while reducing symptoms such as diarrhea and constipation. Chinese patent CN115299533A discloses "an edible composition for pet hair removal containing enzyme complexes and its preparation". The composite enzyme preparation contains keratinase, bromelain, trypsin, pepsin, recombinant disulfide oxidoreductase, serine endopeptidase, and papain. This method also degrades hairballs from the body to achieve the purpose of improving hairball disease.

[0006] Although enzyme preparations containing keratinase can degrade hair, there are few cases of its actual application in improving hairball disease. There are two main reasons for this: 1. The traditional view is that the main site of hairball disease is the stomach; 2. The best working condition of keratinase is a moderately alkaline environment, and it is easy to be inactivated under acidic conditions. In response to the above issues, we launched a comprehensive investigation.

[0007] Through communication with doctors from more than 200 pet hospitals, it was found that the frequency of cat vomiting is positively correlated with the hair content from the pylorus to the front end of the small intestine observed by abdominal examination (X-ray, B-ultrasound, etc.), but has nothing to do with the hair content in the stomach. Cats with severe hairballs can reduce vomiting / hair vomiting for a long time after surgery to remove intestinal hair. At the same time, cats with hairballs can also be clearly observed to have constipation and hair in their feces. Therefore, hairballs often occur in the digestive tract after the stomach, especially the pylorus and small intestine. Existing hairball-eliminating products are mainly dietary fiber and oil substances. Their properties of reducing gastric emptying and promoting intestinal peristalsis also confirm that hair accumulated in the intestines (rather than the stomach) is the main cause of hairballs.

[0008] The pH of a cat's small intestine is between 6 and 7, and most enzyme preparations can exert their activity under this condition. However, enzyme preparations need to pass through the stomach before reaching the small intestine. Gastric acid and pepsin can inactivate most proteins, and even a small amount of enzyme preparations can hardly exert their activity even if they reach the intestine. After the inventors evaluated the gastric acid resistance of 12 types of keratinase on the market, the loss rates were all > 99%. It can be seen that the main technical problem of the current application of enzyme preparations in the direction of hairball removal is how to safely and stably reach the intestine and exert their effects. Fixed-point release and sustained-release technologies can make functional substances be released at a fixed point or slowly in the intestine, which is expected to achieve a breakthrough in enzyme preparations in the field of pet hairball removal, but there are no reports and applications of fixed-point release and sustained-release technologies in hairball removal enzyme preparations.

[0009] In addition, the degradation of hair by a single keratinase requires a mass ratio of enzyme to hair of about 1, which is difficult to achieve in large doses in actual hairball products. Researchers have found that there is an interaction between keratinase and thiol protease, and under the conditions of a composite preparation, hair degradation can be achieved when the ratio of enzyme to hair is 0.01 to 0.1, greatly reducing the amount of enzyme preparation used. Summary of the invention

[0010] The present invention aims to solve the technical problems in the prior art and provides a protease preparation for preventing pet hairball disease and a preparation method and application thereof.

[0011] To achieve the above purpose, the specific technical solution of the present invention is as follows:

[0012] In a first aspect, the present invention provides a protease preparation for preventing pet hairball disease, and a preparation method and application thereof. The protease preparation comprises complex enzymes, vitamins, trace elements, dietary fiber, probiotics, meat powder, and wall materials.

[0013] The mass ratio of the complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall material in the protease preparation is (1-100):(0.1-1):(0.1-10):(1-70):(0.1-10):(10-60):(10-60).

[0014] Preferably, the mass ratio of complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall material in the protease preparation is (10-20):(0.3-0.6):(0.5-5):(5-50):(0.5-1):(20-40):(15-30).

[0015] Further preferably, the mass ratio of complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall material in the protease preparation is (10-20):(0.3-0.6):(0.5-2):(20-40):(0.5-1):(20-40):(15-30).

[0016] More preferably, the mass ratio of complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall material in the protease preparation is 18:0.4:1:30:0.6:30:20.

[0017] The components of the complex enzyme include keratinase, ficin and actinidia protease.

[0018] The mass ratio of keratinase, ficin and actinidia protease in the composite enzyme is (5-80):(1-30):(1-30).

[0019] Preferably, the mass ratio of keratinase, ficin and actinidia protease in the complex enzyme is 60:(20-40):(20-40).

[0020] More preferably, the mass ratio of keratinase, ficin and actinidia protease in the complex enzyme is 60:20:20 (3:1:1).

[0021] Preferably, the complex enzyme also includes one or more of serine protease, papain, bromelain, trypsin and pepsin.

[0022] The vitamins are vitamin A, vitamin B6, vitamin B 12 , vitamin D, lycopene, and folic acid.

[0023] The trace elements include one or more of calcium, magnesium, iron, zinc and copper.

[0024] The dietary fiber is one or more of barley grass powder, astragalus polysaccharide, salvia miltiorrhiza polysaccharide, tuckahoe polysaccharide, oligofructose, inulin, oligoxylose and oligogalactose.

[0025] The probiotics are one or more of Lactobacillus plantarum, Lactobacillus casei, Pediococcus acidilactici, Pediococcus pentosaceus, Enterococcus faecium, Saccharomyces boulardii, Bacillus licheniformis and / or fermentation products thereof.

[0026] The wall material is one or more of calcium alginate, hydroxypropyl cellulose, hydroxypropyl methylcellulose, polyacrylic acid resin, polyvinyl pyrrolidone, copovidone, cellulose acetate phthalate, hydroxypropyl methylcellulose phthalate, polyvinyl phthalate, styrene maleic acid copolymer, ethyl cellulose, cellulose acetate, methyl cellulose, hydroxypropyl methylcellulose acetate succinate, gelatin, sodium carboxymethyl cellulose, polyvinyl alcohol, polyhydroxymethylacrylate alkyl ester, and polyethylene glycol.

[0027] Preferably, the wall material is one or more of ethyl cellulose, hydroxypropyl cellulose, and hydroxypropyl methylcellulose phthalate.

[0028] Preferably, the wall material is hydroxypropyl cellulose.

[0029] The second aspect of the present invention provides a method for preparing a protease preparation for preventing pet hairball disease, and the preparation steps are as follows: weigh each raw material by mass, mix the complex enzyme, probiotics and auxiliary materials (vitamins, trace elements, dietary fiber, meat powder) and granulate them, then spray coat them with wall materials, and finally mix them with other ingredients and press them into tablets.

[0030] The third aspect of the present invention provides an application of a protease preparation for preventing hairballs in pets, wherein the protease preparation is used to degrade hair and difficult-to-degrade food, thereby avoiding the formation of balls of hair and food residues that block the pet's gastrointestinal tract and preventing the occurrence of hairballs.

[0031] Preferably, the pet is a dog or cat, more preferably a cat.

[0032] Beneficial effects:

[0033] 1. The present invention finds that kiwi protease and ficin can cooperate with keratinase to degrade hair, and play the role of degrading hair at a low dose.

[0034] 2. Vitamin B6 and vitamin B selected by the present invention 12, folic acid, etc. have the effect of relieving stress and reducing habitual vomiting; the selected trace elements such as calcium, magnesium, iron, zinc, etc. have the effect of beautifying hair and reducing hair loss, and effectively reduce cats' hair licking behavior and hair intake.

[0035] 3. The present invention finds that hair degraded by keratinase is small insoluble crystalline fragments, which are still not conducive to intestinal peristalsis and easily cause constipation; the selected dietary fiber and probiotics have the function of regulating intestinal flora and promoting defecation, which can effectively solve the constipation problem induced by hair degradation.

[0036] 4. The wall material used in the present invention not only has a coating effect, which can prevent the enzyme preparation and probiotics from being directly exposed to gastric juice and pepsin, greatly ensuring that the enzyme preparation and probiotics safely reach the intestine and play a role in degrading hair and improving the intestine, but also has a long-term sustained release effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 To simulate the degradation of hair by different compound enzyme preparations;

[0038] Figure 2 This is a microscopic observation of the hair degradation simulated by different complex enzyme preparations;

[0039] Figure 3 The figure shows the disintegration effect of enzyme preparations coated with different wall materials in artificial gastric juice;

[0040] Figure 4 It is a visual diagram of the degradation of hair by enzyme preparations coated with different wall materials;

[0041] Figure 5 Microscopic observation of hair degradation by enzyme preparations coated with different wall materials. DETAILED DESCRIPTION

[0042] The above contents will be further described in detail below through specific implementation methods in the form of embodiments. However, this should not be understood as the scope of the above subject matter being limited to the following embodiments. All technologies realized based on the contents of the present invention are within the scope.

[0043] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods; the reagents, materials, etc. used in the following examples, unless otherwise specified, can be obtained from commercial channels.

[0044] Example 1

[0045] A protease preparation for preventing pet hairball disease, wherein the mass ratio of complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall material in the protease preparation is 10:0.3:0.5:5:0.5:20:15.

[0046] Wherein, the compound vitamins are: vitamin A, vitamin B6, vitamin B 12 , vitamin D, lycopene and folic acid are in a weight ratio of 800:1400:3:10:18000:400.

[0047] The trace elements are calcium carbonate, magnesium sulfate, ferrous sulfate, zinc sulfate and copper sulfate in a weight ratio of 1000:300:20:10:1.

[0048] The dietary fiber comprises barley grass powder, astragalus polysaccharide, fructo-oligosaccharide and xylo-oligosaccharide in a weight ratio of 5:4:2:3.

[0049] The mass ratio of keratinase, ficin and actinidia protease in the complex enzyme is 3:2:1.

[0050] The probiotics are: Lactobacillus plantarum, Lactobacillus casei, Pediococcus acidilactici, Pediococcus pentosaceus and Saccharomyces boulardii in a weight ratio of 1:1:1:1:1:1.

[0051] Preparation method: weigh the raw materials according to the above mass parts, mix the complex enzyme and probiotics and granulate them, then spray-coat them with hydroxypropyl cellulose, and finally mix them with vitamins, trace elements, dietary fiber and meat powder and press them into tablets.

[0052] Example 2

[0053] A protease preparation for preventing pet hairball disease, wherein the mass ratio of complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall material in the protease preparation is 10:0.6:0.5:5:1:20:15.

[0054] Wherein, the compound vitamins are: vitamin A, vitamin B6, vitamin B 12 , vitamin D, lycopene and folic acid are in a weight ratio of 700:1500:5:20:20000:500.

[0055] The trace elements are: calcium carbonate, magnesium sulfate, ferrous sulfate, zinc sulfate, and copper sulfate in a weight ratio of 8000:200:15:8:0.8.

[0056] The dietary fiber comprises barley grass powder, astragalus polysaccharide, fructooligosaccharide and xylo-oligosaccharide in a weight ratio of 4:2:3:2.

[0057] The mass ratio of keratinase, ficin and actinidia protease in the complex enzyme is 3:1:2.

[0058] The probiotics are: Lactobacillus plantarum, Lactobacillus casei, Pediococcus acidilactici, Pediococcus pentosaceus and Saccharomyces boulardii in a weight ratio of 2:1:2:2:1.

[0059] Preparation method: weigh the raw materials according to the above mass parts, mix the complex enzyme and probiotics and granulate them, then spray-coat them with hydroxypropyl cellulose, and finally mix them with vitamins, trace elements, dietary fiber and meat powder and press them into tablets.

[0060] Example 3

[0061] A protease preparation for preventing pet hairball disease, wherein the mass ratio of complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall material in the protease preparation is 10:0.6:0.5:50:0.5:40:15.

[0062] Wherein, the compound vitamins are: vitamin A, vitamin B6, vitamin B 12 , vitamin D, lycopene and folic acid are in a weight ratio of 800:1000:5:10:15000:200.

[0063] The trace elements are calcium carbonate, magnesium sulfate, ferrous sulfate, zinc sulfate and copper sulfate in a weight ratio of 1200:500:40:20:1.

[0064] The dietary fiber comprises barley grass powder, astragalus polysaccharide, fructooligosaccharide and xylo-oligosaccharide in a weight ratio of 5:5:3:3.

[0065] The mass ratio of keratinase, ficin and actinidia protease in the complex enzyme is 2:1:1.

[0066] The probiotics are: Lactobacillus plantarum, Lactobacillus casei, Pediococcus acidilactici, Pediococcus pentosaceus and Saccharomyces boulardii in a weight ratio of 3:2:1:2:1.

[0067] Preparation method: weigh the raw materials according to the above mass parts, mix the complex enzyme and probiotics and granulate them, then spray-coat them with hydroxypropyl cellulose, and finally mix them with vitamins, trace elements, dietary fiber and meat powder and press them into tablets.

[0068] Example 4

[0069] A protease preparation for preventing pet hairball disease, wherein the mass ratio of complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall material in the protease preparation is 20:0.3:5:5:1:20:30.

[0070] Wherein, the compound vitamins are: vitamin A, vitamin B6, vitamin B 12 , vitamin D, lycopene and folic acid are in a weight ratio of 500:2000:1:20:18000:400.

[0071] The trace elements are calcium carbonate, magnesium sulfate, ferrous sulfate, zinc sulfate and copper sulfate in a weight ratio of 1000:500:25:17:0.8.

[0072] The dietary fiber comprises barley grass powder, astragalus polysaccharide, oligofructose and oligoxylose in a weight ratio of 4:7:2:3.

[0073] The mass ratio of keratinase, ficin and actinidia protease in the complex enzyme is 3:2:2.

[0074] The probiotics are: Lactobacillus plantarum, Lactobacillus casei, Pediococcus acidilactici, Pediococcus pentosaceus, and Saccharomyces boulardii in a weight ratio of 3:1:2:3:1.

[0075] Preparation method: weigh the raw materials according to the above mass parts, mix the complex enzyme and probiotics and granulate them, then spray-coat them with hydroxypropyl cellulose, and finally mix them with vitamins, trace elements, dietary fiber and meat powder and press them into tablets.

[0076] Example 5

[0077] A protease preparation for preventing pet hairball disease, wherein the mass ratio of complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall material in the protease preparation is 18:0.4:1:30:0.6:30:20.

[0078] Wherein, the compound vitamins are: vitamin A, vitamin B6, vitamin B 12 , vitamin D, lycopene and folic acid are in a weight ratio of 400:700:6:20:10000:600.

[0079] The trace elements are calcium carbonate, magnesium sulfate, ferrous sulfate, zinc sulfate and copper sulfate in a weight ratio of 800:400:40:20:1.5.

[0080] The dietary fiber comprises barley grass powder, astragalus polysaccharide, fructo-oligosaccharide and xylo-oligosaccharide in a weight ratio of 6:5:3:3.

[0081] The mass ratio of keratinase, ficin and actinidia protease in the complex enzyme is 3:1:1.

[0082] The probiotics are: Lactobacillus plantarum, Lactobacillus casei, Pediococcus acidilactici, Pediococcus pentosaceus, and Saccharomyces boulardii in a weight ratio of 2:1:1:3:3.

[0083] Preparation method: weigh the raw materials according to the above mass parts, mix the complex enzyme and probiotics and granulate them, then spray-coat them with hydroxypropyl cellulose, and finally mix them with vitamins, trace elements, dietary fiber and meat powder and press them into tablets.

[0084] Comparative Example 1

[0085] Similar to the formulation of Example 5, the difference is that the complex enzyme preparation (the mass ratio of keratinase, ficinase and actinidia protease is 3:1:1) is replaced by an equal amount of keratinase, and the other components and proportions are exactly the same.

[0086] Comparative Example 2

[0087] Similar to the formulation of Example 5, the difference is that the complex enzyme preparation (the mass ratio of keratinase, ficin and actinidia protease is 3:1:1) is replaced by equal amounts of ficin and actinidia protease, the ratio is 2.5:2.5, and the other components and ratios are exactly the same.

[0088] Comparative Example 3

[0089] The formula is similar to that of Example 5, except that the wall material is replaced by an equal amount of ethyl cellulose instead of hydroxypropyl cellulose, and the other components and proportions are exactly the same.

[0090] Comparative Example 4

[0091] The formula is similar to that of Example 5, except that the wall material is replaced by an equal amount of hydroxypropyl methylcellulose phthalate instead of hydroxypropyl cellulose, and the other components and proportions are exactly the same.

[0092] Comparative Example 5

[0093] The formula is similar to that of Example 5, except that the wall material hydroxypropyl cellulose is replaced with an equal amount of maltodextrin, and the other components and proportions are exactly the same.

[0094] Test Example 1

[0095] Test materials: cat hair, composite enzyme preparations of Examples 1 to 5 of the present invention, composite enzyme preparations of Comparative Examples 1 to 2 of the present invention, PBS buffer (pH=6.5), test tubes, and a constant temperature water bath;

[0096] Experimental treatment: 0.01 g of cat hair was weighed and added to 7 test tubes, 0.5 g of the composite enzyme preparations in Examples 1 to 5 of the present invention and Comparative Examples 1 to 2 were added to each test tube, PBS buffer was added to 20 mL, the hair was immersed, and the mixture was heated in a water bath at 38° C. for 24 h, and the decomposition of the hair was observed;

[0097] For the decomposition of hair after the reaction, see Figures 1-2 ;

[0098] from Figure 1 It can be seen that the composite enzyme preparations in Examples 1 to 5 have a degradation effect on cat hair, while the enzyme preparations in Comparative Examples 1 to 2 have no significant degradation effect on cat hair. Figure 2 From the microscope observation diagram, it can be seen that the enzyme preparation in Comparative Example 2 has no effect on degrading cat hair. In comparison, the enzyme preparation in Comparative Example 1 has an effect on degrading cat hair, but the effect is not obvious. Figure 2 In the experiment, the composite enzyme preparations of Examples 1 to 5 all observed obvious cat hair degradation effects, among which the hair degradation effect of Example 5 was the best.

[0099] Test Example 2

[0100] Test materials: cat hair, artificial gastric juice, the composite enzyme preparation of Example 5 of the present invention, the composite enzyme preparations of Comparative Examples 3 to 5 of the present invention, NaOH solution (0.1 M), test tubes, and a constant temperature water bath;

[0101] Test treatment: Take 0.5g of the composite enzyme preparation in Example 5 of the present invention and Comparative Examples 3 to 5, treat in 37℃ artificial gastric juice (15mL) for 2h, take pictures and observe. Adjust the pH to 6.5 with NaOH solution, and add water to 20mL. Weigh 0.01g of cat hair respectively and add it to each test tube, immerse the hair, shake well, heat in a 38℃ water bath for 24h, and observe the decomposition of the hair;

[0102] The effect of gastric acid treatment is Figure 3 , the hair decomposition after the reaction can be seen in Figures 4-5 ;

[0103] from Figure 3 It can be seen that the enzyme preparation of Comparative Example 5 without using a coating wall material will dissolve quickly in artificial gastric juice without a sustained release effect, and the enzyme preparations of Comparative Examples 3, 4 and Example 5 all have good gastric protection effects. In comparison, the gastric protection effect of Example 5 using hydroxypropyl cellulose as a wall material is better than that of Comparative Example 3 using ethyl cellulose, and the effect of Comparative Example 3 using ethyl cellulose as a wall material is better than that of Comparative Example 4 using hydroxypropyl methylcellulose phthalate.

[0104] from Figure 4 and Figure 5 It can be seen that the enzyme preparation without wall material coating (Comparative Example 5) lost the ability to degrade cat hair after being treated with artificial gastric juice, and the enzyme preparations with wall material coating all showed the effect of degrading hair balls. Comprehensive comparison shows that Example 5 is better than Comparative Example 3, and both are better than Comparative Example 4.

[0105] Test Example 3 Clinical Hairball Removal Effect Test

[0106] Animal tests were conducted using the complex enzyme sustained-release tablets in Example 5;

[0107] The experimental treatment and feeding method described: 30 British shorthair cats with hairball disease were selected from multiple pet stray rescue stations in Tianjin and divided into 3 groups, namely a blank group, a control group and an experimental group, with 10 cats in each group. The blank group was fed a normal diet (no other treatment), the control group was orally fed with commercially available hairball tablets every day, and the experimental group was orally fed with the hairball preparation prepared in Example 5 every day. The control and experimental groups were fed 2 tablets each morning and evening, and were free to eat and drink water. The experimental period was 30 days, and the number of vomiting cats, vomiting frequency, feces texture and integrity of hair contained, and food intake were recorded every day;

[0108] Symptoms of the cats in the blank group: coarse and dull fur, about 1 / 3 of the time spent licking fur, vomiting frequency about 4 times / month, with a small amount of hair in the vomit, frequent retching, 1-2 bowel movements per day, dry and hard feces, with a small amount of intact hair in the feces;

[0109] The symptoms of the cats in the control group: the roughness and color of the hair improved, about 1 / 3 of the time was spent licking the hair (slightly decreased but not obvious), the frequency of vomiting decreased to 1-2 times / month (in the early stage), a small amount of hair was found in the vomit, and the cats defecated 3-6 times a day, mostly soft and unformed stools with intact hair in the stool;

[0110] Symptoms of cats in the experimental group: hair roughness and color improved, about 1 / 3 of the time spent licking hair (decreased but not significantly), average vomiting frequency less than 1 time / month (some cats vomited once in the early stage), a small amount of hair in vomit, 2-3 bowel movements per day, normal stool texture, no obvious hair or short hair visible in the stool;

[0111] Commercially available hair ball removers have a good effect on improving hair gloss and reducing the frequency of vomiting, but they can cause the British Shorthair cat's defecation pattern to change from constipation to diarrhea. Compared with commercially available hair ball removers, the hair ball removers of the present invention reduce the frequency of vomiting, the defecation volume and frequency return to normal, the texture of the feces is improved, and the degradation of hair in the feces can be significantly observed.

Claims

1. A protease preparation for preventing pet hairball disease, characterized in that: The protease preparation comprises complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall materials; the mass ratio of the complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall materials in the protease preparation is (1-100):(0.1-1):(0.1-10):(1-70):(0.1-10):(10-60):(10-60).

2. The protease preparation according to claim 1, characterized in that The mass ratios of the complex enzyme, vitamins, trace elements, dietary fiber, probiotics, meat powder and wall material in the protease preparation are (10-20):(0.3-0.6):(0.5-5):(5-50):(0.5-1):(20-40):(15-30).

3. The protease preparation according to claim 1 or 2, characterized in that The components of the composite enzyme include keratinase, ficin and kiwi protease, and the mass ratio of keratinase, ficin and kiwi protease in the composite enzyme is (5-80):(1-30):(1-30).

4. The protease preparation according to claim 3, characterized in that The mass ratio of keratinase, ficin and actinidia protease in the composite enzyme is 60:(20-40):(20-40).

5. The protease preparation according to claim 1 or 2, characterized in that The vitamins are vitamin A, vitamin B6, vitamin B 12 , vitamin D, lycopene, folic acid; the trace elements include one or more of calcium, magnesium, iron, zinc, and copper; the dietary fiber is one or more of barley grass powder, astragalus polysaccharide, salvia miltiorrhiza polysaccharide, tuckahoe polysaccharide, oligofructose, inulin, oligoxylose, and oligogalactose; the probiotics are one or more of Lactobacillus plantarum, Lactobacillus casei, Pediococcus acidilactici, Pediococcus pentosaceus, Enterococcus faecalis, Saccharomyces boulardii, Bacillus licheniformis and / or their fermentation products.

6. The protease preparation according to claim 1 or 2, characterized in that The wall material is one or more of calcium alginate, hydroxypropyl cellulose, hydroxypropyl methylcellulose, polyacrylic acid resin, polyvinyl pyrrolidone, copovidone, cellulose acetate phthalate, hydroxypropyl methylcellulose phthalate, polyvinyl phthalate, styrene maleic acid copolymer, ethyl cellulose, cellulose acetate, methyl cellulose, hydroxypropyl methylcellulose acetate succinate, gelatin, sodium carboxymethyl cellulose, polyvinyl alcohol, polyhydroxymethylacrylate alkyl ester, and polyethylene glycol.

7. The protease preparation according to claim 6, characterized in that The wall material is one or more of ethyl cellulose, hydroxypropyl cellulose and hydroxypropyl methyl cellulose phthalate.

8. The protease preparation according to claim 7, characterized in that The wall material is hydroxypropyl cellulose.

9. Use of a protease preparation for preventing pet hairball disease as claimed in any one of claims 1 to 8 in degrading hair and difficult-to-degrade food, characterized in that: Avoid the formation of balls of hair and non-degradable food residues that may block the pet's gastrointestinal tract and prevent the occurrence of hairball disease.

10. The use according to claim 9, characterized in that: The pet is a cat.

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