Ruminant premix feed composition containing bacillus licheniformis

Abstract

The invention belongs to the technical field of animal feed, and relates to a ruminant premix feed composition containing bacillus licheniformis. The premixed feed composition comprises the following components in percentage by mass: 12-20% of Chinese herbal medicines, 1.5-3.0% of compound amino acid, 36-50% of compound trace elements, 3.0-5.0% of a microecological preparation and 32-40% of a carrier. By adding the premix feed composition into daily ration, the IgG content of the calves is remarkably increased, diarrhea is effectively reduced, the body weight is increased, rapid growth of the calves is promoted, and the breeding benefits of the calves are remarkably increased.

Description

Technical Field

[0001] The invention belongs to the technical field of animal feed, and relates to a ruminant premix feed composition containing Bacillus licheniformis, and a preparation method and application thereof. Background Art

[0002] Calf diarrhea is a digestive system disease, usually caused by excessive intestinal peristalsis, malabsorption of intestinal fluid or increased secretion of intestinal fluid. The causes of calf diarrhea can be divided into two categories: feeding and management factors and pathogenic microorganisms. Calf diarrhea may occur to varying degrees at different ages and in different seasons. Calf diarrhea is one of the serious diseases that affects the growth and development of calves and even causes the death of calves. It brings huge economic losses to farms every year. Reducing the incidence of diarrhea is of great significance to the health of cattle and ranch benefits.

[0003] Premix feed is a uniform mixture of two or more feed additives and carriers or diluents prepared in a certain proportion. Its purpose is to provide compound feed with nutritional elements such as vitamins, trace elements, amino acids, etc., as well as to improve feed quality and increase digestibility.

[0004] As a type of animal dietary additive, probiotics stabilize the intestinal flora structure, prevent pathogens from entering, and restore the intestinal microecological system to a healthy state when they enter the animal body. my country's Ministry of Agriculture and Rural Affairs lists 16 microorganisms of microecological preparations, such as Bacillus licheniformis, Bacillus subtilis, and Streptococcus faecalis, as feed-grade microbial additives. Different probiotics have different effects on animal bodies.

[0005] Chinese herbal medicine has the advantages of multiple functions, little toxic side effects, and not easy to develop drug resistance. With the implementation of the comprehensive "antibiotic ban" policy, its application in the prevention and treatment of calf diarrhea has become increasingly widespread.

[0006] It is of great significance for calf breeding to study a premixed feed with added probiotics and Chinese herbal medicine to enhance calf immunity and prevent calf diarrhea. Summary of the invention

[0007] In view of this, the object of the present invention is to provide a ruminant premix feed composition containing Bacillus licheniformis. The premix feed composition of the present invention enhances the immune function of ruminants, promotes growth, and significantly improves the breeding efficiency of animals.

[0008] To achieve the above objectives, this application adopts the following technical solutions: In a first aspect, the present invention provides a ruminant premix feed composition containing Bacillus licheniformis, wherein the premix feed composition comprises, by weight percentage, 12-20% of Chinese herbal medicine, 1.5-3.0% of complex amino acids, 36-50% of complex trace elements, 3.0-5.0% of probiotic preparations, and 32-40% of carriers; the Chinese herbal medicine is composed of the following parts by weight: 8-12 parts of Atractylodes macrocephala, 8-12 parts of Angelica sinensis, 8-12 parts of Astragalus membranaceus, 8-12 parts of Radix Angelicae Sinensis, 8-12 parts of Radix Astragali, 8-12 parts of Radix Angelicae Sinensis ... 4-6 parts, Echinacea purpurea 8-12 parts, white peony root 4-6 parts, Codonopsis pilosula 4-6 parts, tangerine peel 8-12 parts and liquorice 4-6 parts; the composite amino acid consists of lysine, methionine and arginine in a mass ratio of 1-3:2-4:1; the composite trace element consists of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 1-3:1:3-5; the proecological preparation consists of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 3-5:1-3:2-4; the carrier is soybean meal or bone meal.

[0009] Furthermore, in the microecological preparation, the amount of live bacteria of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum is 1×10 10 CFU / g.

[0010] In one embodiment of the present invention, the premix feed composition comprises, by mass percentage, 15% Chinese herbal medicine, 2.0% complex amino acids, 45% complex trace elements, 3.0% probiotic preparations and 35% carriers; the Chinese herbal medicines are composed of the following parts by weight: 10 parts of Atractylodes macrocephala, 10 parts of Angelica sinensis, 5 parts of Astragalus membranaceus, 10 parts of Echinacea purpurea, 5 parts of White Peony Root, 5 parts of Codonopsis pilosula, 10 parts of Tangerine Peel and 5 parts of Licorice; the complex amino acids are composed of lysine, methionine and arginine in a mass ratio of 2:3:1; the complex trace elements are composed of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 2:1:4; the probiotic preparations are composed of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 4:2:3; and the carrier is bone meal.

[0011] In one embodiment of the present invention, the premix feed composition comprises, by weight percentage, 10% Chinese herbal medicine, 3.0% complex amino acids, 50% complex trace elements, 2.5% probiotic preparations and 30% carriers; the Chinese herbal medicines are composed of the following parts by weight: 12 parts of Atractylodes macrocephala, 8 parts of Angelica sinensis, 6 parts of Astragalus membranaceus, 8 parts of Echinacea purpurea, 6 parts of White Peony Root, 4 parts of Codonopsis pilosula, 8 parts of Tangerine Peel and 6 parts of Licorice; the complex amino acids are composed of lysine, methionine and arginine in a mass ratio of 1:2:1; the complex trace elements are composed of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 1:1:3; the probiotic preparations are composed of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 3:1:2; and the carrier is soybean meal.

[0012] In one embodiment of the present invention, the premix feed composition comprises, by weight percentage, 20% Chinese herbal medicine, 1.5% complex amino acid, 40% complex trace element, 5.0% probiotic preparation and 40% carrier; the Chinese herbal medicine is composed of the following parts by weight: 8 parts of Atractylodes macrocephala, 12 parts of Angelica sinensis, 4 parts of Astragalus membranaceus, 12 parts of Echinacea purpurea, 4 parts of White Peony Root, 6 parts of Codonopsis pilosula, 12 parts of Tangerine Peel and 4 parts of Licorice; the complex amino acid is composed of lysine, methionine and arginine in a mass ratio of 3:4:1; the complex trace element is composed of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 3:1:3; the probiotic preparation is composed of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 5:3:4; and the carrier is bone meal.

[0013] In a second aspect, the present invention provides a method for preparing the above-mentioned premix feed composition, which comprises: premixing a formula amount of Chinese herbal medicines Atractylodes macrocephala, Angelica sinensis, Astragalus membranaceus, Echinacea purpurea, White Peony Root, Codonopsis pilosula, Tangerine peel and Licorice, and then crushing the mixture, passing through an 80-mesh sieve to obtain a Chinese herbal medicine complex; and uniformly mixing the Chinese herbal medicine complex with other components according to the formula amount to obtain a ruminant premix feed composition containing Bacillus licheniformis.

[0014] In a third aspect, the present invention provides the use of the above-mentioned Bacillus licheniformis-containing ruminant premix feed composition or the Bacillus licheniformis-containing ruminant premix feed composition prepared by the above-mentioned preparation method in ruminant feed.

[0015] Furthermore, the present invention provides the above-mentioned Bacillus licheniformis-containing ruminant premix feed composition or the Bacillus licheniformis-containing ruminant premix feed composition prepared by the above-mentioned preparation method to enhance the immune function of ruminants and promote growth.

[0016] Furthermore, the dosage of the ruminant premix feed composition containing Bacillus licheniformis is 4.5% of the basic diet.

[0017] Chinese herbal medicine can directly act on bacteria and destroy their cell membrane structure, thereby achieving antibacterial effects; it can also inhibit bacterial growth by interfering with bacterial sugar metabolism, hindering RNA or DNA synthesis, etc. In addition, the various immune active substances contained in Chinese herbal medicine can also indirectly exert antibacterial effects by improving immune function.

[0018] After probiotics enter the body of ruminants, they limit the colonization, survival and reproduction of harmful bacteria in the intestines through temporal and spatial competition and nutritional competition with the adhesion sites of pathogenic bacteria, thereby supporting the growth of beneficial bacteria. By controlling the ratio of beneficial bacteria to harmful bacteria, they regulate the balance of microbial colonies in the animal body, regulate the intestinal health of calves, improve digestive function, and reduce the incidence of diarrhea.

[0019] The various probiotics and Chinese herbal medicines in the premixed feed composition of the present invention act synergistically to enhance the immune function of animals, reduce the occurrence of diarrhea, promote growth, and significantly improve the breeding efficiency of animals.

[0020] Beneficial effects achieved by the present invention: The content of immunoglobulin in calf serum can directly reflect the calf's ability to resist disease and certain disease states, and can also reflect the body's protein metabolism level to a certain extent, and indirectly reflect the body's immune ability. The proteins involved in humoral immunity in animals are mainly IgG, IgM and IgA. Immunoglobulins have biological functions such as binding antigens and activating complement. Improving the levels of IgG, IgM and IgA in animals can enhance their health.

[0021] Adding the premixed feed composition of the present invention into the diet significantly increases the IgG content of calves, effectively reduces the occurrence of calf diarrhea, and increases the weight of calves.

[0022] The premixed feed composition of the invention enhances the immune function of animals, promotes growth, and significantly improves the breeding efficiency of calves. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the drawings required for the specific implementation methods are briefly introduced below.

[0024] Figure 1 Comparison of body weight among groups Compared with the CK group, * P<0.05, ** P<0.01; compared with group A, # P<0.05.

[0025] Figure 2 Comparison of IgG content in each group Compared with the CK group, * P<0.05, ** P<0.01; compared with group A, # P<0.05, ## P<0.01.

[0026] Figure 3 Comparison of IgM content in each group.

[0027] Figure 4 Comparison of IgA content in each group.

[0028] Figure 5 Comparison of diarrhea frequency among groups Compared with the CK group, * P<0.05, ** P<0.01; compared with group A, #P<0.05.

[0029] Figure 6 Comparison of stool scores among groups. DETAILED DESCRIPTION

[0030] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0031] Example 1 A ruminant premix feed composition containing Bacillus licheniformis comprises, by mass percentage, 150 g of Chinese herbal medicine, 20 g of complex amino acids, 450 g of complex trace elements, 30 g of a probiotic preparation and 350 g of a carrier.

[0032] The Chinese herbal medicine consists of the following parts by weight: 10 parts of Atractylodes macrocephala, 10 parts of Angelica sinensis, 5 parts of Astragalus membranaceus, 10 parts of Echinacea purpurea, 5 parts of White Peony Root, 5 parts of Codonopsis pilosula, 10 parts of Tangerine Peel and 5 parts of Licorice.

[0033] The composite amino acid consists of lysine, methionine and arginine in a mass ratio of 2:3:1.

[0034] The composite trace element consists of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 2:1:4.

[0035] The microecological preparation is composed of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 4:2:3, and the live bacterial counts of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum are 1×10 10 CFU / g.

[0036] The carrier is bone meal.

[0037] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines such as Atractylodes macrocephala, Angelica sinensis, Astragalus membranaceus, Echinacea purpurea, White Peony Root, Codonopsis pilosula, Tangerine peel and Licorice, and then crushing the mixture and passing it through an 80-mesh sieve to obtain a Chinese herbal medicine complex; and uniformly mixing the Chinese herbal medicine complex with other components according to the formula amount to obtain a ruminant premix feed composition containing Bacillus licheniformis.

[0038] Example 2 A ruminant premix feed composition containing Bacillus licheniformis comprises, by mass percentage, 100 g of Chinese herbal medicine, 30 g of complex amino acids, 500 g of complex trace elements, 25 g of a probiotic preparation and 300 g of a carrier.

[0039] The Chinese herbal medicine consists of the following parts by weight: 12 parts of Atractylodes macrocephala, 8 parts of Angelica sinensis, 6 parts of Astragalus membranaceus, 8 parts of Echinacea purpurea, 6 parts of White Peony Root, 4 parts of Codonopsis pilosula, 8 parts of Tangerine Peel and 6 parts of Licorice.

[0040] The composite amino acid consists of lysine, methionine and arginine in a mass ratio of 1:2:1.

[0041] The composite trace element consists of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 1:1:3.

[0042] The microecological preparation consists of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 3:1:2.

[0043] The carrier is soybean meal.

[0044] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines such as Atractylodes macrocephala, Angelica sinensis, Astragalus membranaceus, Echinacea purpurea, White Peony Root, Codonopsis pilosula, Tangerine peel and Licorice, and then crushing the mixture and passing it through an 80-mesh sieve to obtain a Chinese herbal medicine complex; and uniformly mixing the Chinese herbal medicine complex with other components according to the formula amount to obtain a ruminant premix feed composition containing Bacillus licheniformis.

[0045] Example 3 A ruminant premix feed composition containing Bacillus licheniformis comprises, by mass percentage, 200 g of Chinese herbal medicine, 15 g of complex amino acids, 400 g of complex trace elements, 50 g of a probiotic preparation and 400 g of a carrier.

[0046] The Chinese herbal medicine consists of the following parts by weight: 8 parts of Atractylodes macrocephala, 12 parts of Angelica sinensis, 4 parts of Astragalus membranaceus, 12 parts of Echinacea purpurea, 4 parts of White Peony Root, 6 parts of Codonopsis pilosula, 12 parts of Tangerine Peel and 4 parts of Licorice.

[0047] The composite amino acid consists of lysine, methionine and arginine in a mass ratio of 3:4:1.

[0048] The composite trace element consists of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 3:1:3.

[0049] The microecological preparation consists of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 5:3:4.

[0050] The carrier is bone meal.

[0051] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines such as Atractylodes macrocephala, Angelica sinensis, Astragalus membranaceus, Echinacea purpurea, White Peony Root, Codonopsis pilosula, Tangerine peel and Licorice, and then crushing the mixture and passing it through an 80-mesh sieve to obtain a Chinese herbal medicine complex; and uniformly mixing the Chinese herbal medicine complex with other components according to the formula amount to obtain a ruminant premix feed composition containing Bacillus licheniformis.

[0052] Example 4 A ruminant premix feed composition containing Bacillus licheniformis comprises, by mass percentage, 200 g of Chinese herbal medicine, 30 g of complex amino acids, 450 g of complex trace elements, 30 g of a probiotic preparation and 350 g of a carrier.

[0053] The Chinese herbal medicine consists of the following parts by weight: 10 parts of Atractylodes macrocephala, 8 parts of Angelica sinensis, 6 parts of Astragalus membranaceus, 10 parts of Echinacea purpurea, 5 parts of White Peony Root, 6 parts of Codonopsis pilosula, 8 parts of Tangerine Peel and 4 parts of Licorice.

[0054] The composite amino acid consists of lysine, methionine and arginine in a mass ratio of 1:4:1.

[0055] The composite trace element consists of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 3:1:5.

[0056] The microecological preparation consists of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 3:3:4.

[0057] The carrier is bone meal.

[0058] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines such as Atractylodes macrocephala, Angelica sinensis, Astragalus membranaceus, Echinacea purpurea, White Peony Root, Codonopsis pilosula, Tangerine peel and Licorice, and then crushing the mixture and passing it through an 80-mesh sieve to obtain a Chinese herbal medicine complex; and uniformly mixing the Chinese herbal medicine complex with other components according to the formula amount to obtain a ruminant premix feed composition containing Bacillus licheniformis.

[0059] Example 5 A ruminant premix feed composition containing Bacillus licheniformis comprises, by mass percentage, 100 g of Chinese herbal medicine, 20 g of complex amino acids, 400 g of complex trace elements, 50 g of a probiotic preparation and 400 g of a carrier.

[0060] The Chinese herbal medicine consists of the following parts by weight: 12 parts of Atractylodes macrocephala, 10 parts of Angelica sinensis, 5 parts of Astragalus membranaceus, 12 parts of Echinacea purpurea, 4 parts of White Peony Root, 5 parts of Codonopsis pilosula, 10 parts of Tangerine Peel and 4 parts of Licorice.

[0061] The composite amino acid consists of lysine, methionine and arginine in a mass ratio of 3:2:1.

[0062] The composite trace element consists of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 1:1:5.

[0063] The microecological preparation consists of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 5:1:2.

[0064] The carrier is soybean meal.

[0065] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines such as Atractylodes macrocephala, Angelica sinensis, Astragalus membranaceus, Echinacea purpurea, White Peony Root, Codonopsis pilosula, Tangerine peel and Licorice, and then crushing the mixture and passing it through an 80-mesh sieve to obtain a Chinese herbal medicine complex; and uniformly mixing the Chinese herbal medicine complex with other components according to the formula amount to obtain a ruminant premix feed composition containing Bacillus licheniformis.

[0066] Example 6 A ruminant premix feed composition containing Bacillus licheniformis comprises, by mass percentage, 150 g of Chinese herbal medicine, 15 g of complex amino acids, 400 g of complex trace elements, 50 g of a probiotic preparation and 350 g of a carrier.

[0067] The Chinese herbal medicine consists of the following parts by weight: 8 parts of Atractylodes macrocephala, 8 parts of Angelica sinensis, 6 parts of Astragalus membranaceus, 12 parts of Echinacea purpurea, 4 parts of White Peony Root, 5 parts of Codonopsis pilosula, 8 parts of Tangerine Peel and 6 parts of Licorice.

[0068] The composite amino acid consists of lysine, methionine and arginine in a mass ratio of 1:4:1.

[0069] The composite trace element consists of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 1:1:3.

[0070] The microecological preparation consists of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 3:2:3.

[0071] The carrier is soybean meal.

[0072] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines such as Atractylodes macrocephala, Angelica sinensis, Astragalus membranaceus, Echinacea purpurea, White Peony Root, Codonopsis pilosula, Tangerine peel and Licorice, and then crushing the mixture and passing it through an 80-mesh sieve to obtain a Chinese herbal medicine complex; and uniformly mixing the Chinese herbal medicine complex with other components according to the formula amount to obtain a ruminant premix feed composition containing Bacillus licheniformis.

[0073] Comparative Example 1 Compared with Example 1, the difference lies in that the components of the Chinese herbal medicine are different.

[0074] A ruminant premix feed composition containing Bacillus licheniformis comprises, by mass percentage, 150 g of Chinese herbal medicine, 20 g of complex amino acids, 450 g of complex trace elements, 30 g of a probiotic preparation and 350 g of a carrier.

[0075] The Chinese herbal medicine consists of the following parts by weight: 10 parts of Atractylodes macrocephala, 15 parts of Angelica sinensis, 10 parts of Echinacea purpurea, 10 parts of Codonopsis pilosula, 10 parts of dried tangerine peel and 5 parts of liquorice.

[0076] The composite amino acid consists of lysine, methionine and arginine in a mass ratio of 2:3:1.

[0077] The composite trace element consists of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 2:1:4.

[0078] The microecological preparation is composed of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 4:2:3, and the live bacterial counts of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum are 1×10 10 CFU / g.

[0079] The carrier is bone meal.

[0080] The preparation method is similar to that of Example 1.

[0081] Comparative Example 2 Compared with Example 1, the difference lies in that the components of the Chinese herbal medicine and the components of the composite amino acids are different.

[0082] A ruminant premix feed composition containing Bacillus licheniformis comprises, by mass percentage, 150 g of Chinese herbal medicine, 20 g of complex amino acids, 450 g of complex trace elements, 30 g of a probiotic preparation and 350 g of a carrier.

[0083] The Chinese herbal medicine consists of the following parts by weight: 15 parts of angelica sinensis, 10 parts of astragalus, 10 parts of echinacea, 5 parts of white peony root, 5 parts of codonopsis pilosula, 10 parts of tangerine peel and 5 parts of liquorice.

[0084] The composite amino acid consists of lysine, glycine and threonine in a mass ratio of 2:3:1.

[0085] The composite trace element consists of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 2:1:4.

[0086] The microecological preparation is composed of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 4:2:3, and the live bacterial counts of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum are 1×10 10 CFU / g.

[0087] The carrier is bone meal.

[0088] The preparation method is similar to that of Example 1.

[0089] Comparative Example 3 Compared with Example 1, the difference lies in that the components of the Chinese herbal medicine and the components of the microecological preparation are different.

[0090] A ruminant premix feed composition containing Bacillus licheniformis comprises, by mass percentage, 150 g of Chinese herbal medicine, 20 g of complex amino acids, 450 g of complex trace elements, 30 g of a probiotic preparation and 350 g of a carrier.

[0091] The Chinese herbal medicine consists of the following parts by weight: 10 parts of Atractylodes macrocephala, 10 parts of Angelica sinensis, 10 parts of Astragalus membranaceus, 10 parts of White Peony Root, 5 parts of Codonopsis pilosula, 10 parts of Tangerine Peel and 5 parts of Licorice.

[0092] The composite amino acid consists of lysine, methionine and arginine in a mass ratio of 2:3:1.

[0093] The composite trace element consists of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 2:1:4.

[0094] The microecological preparation is composed of Enterococcus faecalis, Bacillus amyloliquefaciens and Lactobacillus plantarum in a mass ratio of 2:5:2, and the live bacterial counts of Enterococcus faecalis, Bacillus amyloliquefaciens and Lactobacillus plantarum are 1×10 10 CFU / g.

[0095] The carrier is bone meal.

[0096] The preparation method is similar to that of Example 1.

[0097] Comparative Example 4 Compared with Example 1, the difference lies in that the percentages of the components in the premix feed composition are different.

[0098] A ruminant premix feed composition containing Bacillus licheniformis comprises, by mass percentage, 250 g of Chinese herbal medicine, 10 g of complex amino acids, 300 g of complex trace elements, 25 g of a probiotic preparation and 415 g of a carrier.

[0099] The Chinese herbal medicine consists of the following parts by weight: 10 parts of Atractylodes macrocephala, 10 parts of Angelica sinensis, 5 parts of Astragalus membranaceus, 10 parts of Echinacea purpurea, 5 parts of White Peony Root, 5 parts of Codonopsis pilosula, 10 parts of Tangerine Peel and 5 parts of Licorice.

[0100] The composite amino acid consists of lysine, methionine and arginine in a mass ratio of 2:3:1.

[0101] The composite trace element consists of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 2:1:4.

[0102] The microecological preparation is composed of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 4:2:3, and the live bacterial counts of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum are 1×10 10 CFU / g.

[0103] The carrier is bone meal.

[0104] The preparation method is similar to that of Example 1.

[0105] Experimental Example Effect of the Premixed Feed Composition of the Present Invention on Diarrhea and Growth Performance of Calf 1. Experimental Methods 42 newborn local yellow cattle calves aged 20 days were selected and divided into 7 groups according to the principle of similar birth order and birth weight: control group (CK group), embodiment 1 group (A group), embodiment 2 group (B group), comparative example 1 group (C group), comparative example 2 group (D group), comparative example 3 group (E group), comparative example 4 group (F group), 6 calves in each group, the test period was 70 days, including a pre-feeding period of 10 days and a main test period of 60 days. The control group was fed a basic diet, and each test group added 4.5% of the corresponding product to the basic diet.

[0106] The experimental calves were fed in the calf pen before weaning, and each calf had separate water and feed, free access to food and water, and milk was pasteurized before weaning and then artificially fed. They were fed milk twice a day in the morning and evening, 6.5L per day for 20-50 days of age, and 4L per day for 50-60 days of age (weaning). After weaning, they were transferred to free stalls for feeding.

[0107] 2. Determination of indicators 2.1 Weight At 90 days of age, the rats were weighed on an empty stomach (BW).

[0108] 2.2 Serum immune indexes Blood was collected from the vein, centrifuged for 15 min, and serum was extracted and stored at -20°C for testing. The contents of bovine immunoglobulin M (IgM), immunoglobulin A (IgA), and immunoglobulin G (IgG) were determined.

[0109] 2.3 Stool scores and diarrhea incidence Observe the feces of the calves before feeding every morning and score them. The scoring criteria are shown in Table 1. When the feces score of the calf is >2, it is recorded as diarrhea. When the score is 4, treatment is carried out according to the diagnosis and treatment plan.

[0110] Table 1 Fecal scoring criteria The feces index of each group during the trial period = the sum of feces scores / total number of heads.

[0111] Diarrhea frequency (%) = Σ(number of diarrhea cases × number of diarrhea days) / (number of test cases × number of days in the test period) × 100.

[0112] 3. Data processing Graphpad Prism 7.0 software and SPSS 19.0 data processing system were used for data statistical analysis and graphing.

[0113] 4. Test results 4.1 Effect on body weight like Figure 1As shown, compared with the CK group, the treatment of group A and group B significantly increased body weight. The effect of group A on increasing body weight was the most obvious, and the effect of group B on increasing body weight was also significant. The effect of group A and group B on increasing body weight was significantly better than that of group C, group D, group E, and group F.

[0114] 4.2 Effects on serum immune indicators like Figure 2 , 3 As shown in Figures 4 and 5, compared with the CK group, the IgG content in groups A and B was significantly increased. The effect of increasing the IgG content in groups A and B was significantly better than that in groups C, D, E, and F.

[0115] 4.3 Effect on the occurrence of diarrhea like Figure 5 , 6 As shown, compared with the CK group, the treatment of group A and group B reduced the frequency and probability of diarrhea. The effect of group A and group B in reducing the occurrence of diarrhea was significantly better than that of group C, group D, group E, and group F.

[0116] The content of immunoglobulin in calf serum can directly reflect the calf's ability to resist disease and certain disease states, and can also reflect the body's protein metabolism level to a certain extent, and indirectly reflect the body's immune ability. The proteins involved in humoral immunity in animals are mainly IgG, IgM and IgA. Immunoglobulins have biological functions such as binding antigens and activating complement. Improving the levels of IgG, IgM and IgA in animals can enhance their health.

[0117] Adding the premixed feed composition of the present invention into the diet can significantly increase the IgG content of calves, effectively reduce the occurrence of calf diarrhea, and increase the weight of calves.

[0118] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A ruminant premix feed composition containing Bacillus licheniformis, characterized in that: The premixed feed composition comprises, by weight percentage, 12-20% of Chinese herbal medicine, 1.5-3.0% of complex amino acids, 36-50% of complex trace elements, 3.0-5.0% of probiotic preparations and 32-40% of carriers; The Chinese herbal medicine is composed of the following parts by weight: 8-12 parts of Atractylodes macrocephala, 8-12 parts of Angelica sinensis, 4-6 parts of Astragalus, 8-12 parts of Echinacea, 4-6 parts of White Peony Root, 4-6 parts of Codonopsis pilosula, 8-12 parts of Tangerine Peel and 4-6 parts of Licorice; The composite amino acid is composed of lysine, methionine and arginine in a mass ratio of 1-3:2-4:1; The composite trace elements are composed of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 1-3:1:3-5; The microecological preparation is composed of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 3-5:1-3:2-4; The carrier is soybean meal or bone meal.

2. The ruminant premix feed composition containing Bacillus licheniformis according to claim 1, characterized in that: In the microecological preparation, the amount of live bacteria of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum is 1×10 10 CFU / g.

3. The ruminant premix feed composition containing Bacillus licheniformis according to claim 1, characterized in that: The premixed feed composition comprises, by weight percentage, 15% Chinese herbal medicine, 2.0% complex amino acids, 45% complex trace elements, 3.0% probiotic preparations and 35% carriers; The Chinese herbal medicine is composed of the following parts by weight: 10 parts of Atractylodes macrocephala, 10 parts of Angelica sinensis, 5 parts of Astragalus membranaceus, 10 parts of Echinacea purpurea, 5 parts of White Peony Root, 5 parts of Codonopsis pilosula, 10 parts of Tangerine Peel and 5 parts of Licorice; The composite amino acid is composed of lysine, methionine and arginine in a mass ratio of 2:3:1; The composite trace element is composed of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 2:1:4; The microecological preparation is composed of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 4:2:3; The carrier is bone meal.

4. The ruminant premix feed composition containing Bacillus licheniformis according to claim 1, characterized in that: The premixed feed composition comprises, by weight percentage, 10% Chinese herbal medicine, 3.0% compound amino acids, 50% compound trace elements, 2.5% probiotic preparations and 30% carriers; The Chinese herbal medicine is composed of the following parts by weight: 12 parts of Atractylodes macrocephala, 8 parts of Angelica sinensis, 6 parts of Astragalus membranaceus, 8 parts of Echinacea purpurea, 6 parts of White Peony Root, 4 parts of Codonopsis pilosula, 8 parts of Tangerine Peel and 6 parts of Licorice; The composite amino acid is composed of lysine, methionine and arginine in a mass ratio of 1:2:1; The composite trace element is composed of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 1:1:3; The microecological preparation is composed of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 3:1:2; The carrier is soybean meal.

5. The ruminant premix feed composition containing Bacillus licheniformis according to claim 1, characterized in that: The premixed feed composition comprises, by weight percentage, 20% Chinese herbal medicine, 1.5% complex amino acids, 40% complex trace elements, 5.0% probiotic preparations and 40% carriers; The Chinese herbal medicine is composed of the following parts by weight: 8 parts of Atractylodes macrocephala, 12 parts of Angelica sinensis, 4 parts of Astragalus membranaceus, 12 parts of Echinacea purpurea, 4 parts of White Peony Root, 6 parts of Codonopsis pilosula, 12 parts of Tangerine Peel and 4 parts of Licorice; The composite amino acid is composed of lysine, methionine and arginine in a mass ratio of 3:4:1; The composite trace element is composed of zinc sulfate, calcium iodate and sodium selenite in a mass ratio of 3:1:3; The microecological preparation is composed of Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum in a mass ratio of 5:3:4; The carrier is bone meal.

6. The ruminant premix feed composition containing Bacillus licheniformis according to claim 1, characterized in that: The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines such as Atractylodes macrocephala, Angelica sinensis, Astragalus membranaceus, Echinacea purpurea, White Peony Root, Codonopsis pilosula, Tangerine peel and Licorice, and then crushing the mixture and passing it through an 80-mesh sieve to obtain a Chinese herbal medicine complex; and uniformly mixing the Chinese herbal medicine complex with other components according to the formula amount to obtain a ruminant premix feed composition containing Bacillus licheniformis.

7. Use of the ruminant premix feed composition containing Bacillus licheniformis according to any one of claims 1 to 5 or the ruminant premix feed composition containing Bacillus licheniformis prepared by the preparation method according to claim 6 in ruminant feed.

8. The use according to claim 7, characterized in that: The ruminant premix feed composition containing Bacillus licheniformis or the ruminant premix feed composition containing Bacillus licheniformis prepared by the preparation method according to claim 6 enhances the immune function of ruminants and promotes growth.

9. The use according to claim 7, characterized in that: The amount of the ruminant premix feed composition containing Bacillus licheniformis is 4.5% of the basic diet.

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