Formula feed for indoor feeding of yellow rice borers and preparation method of formula feed
By providing a formula feed containing ingredients such as fresh wild rice rice, sugar cane stem powder, the problem of poor indoor feeding effect of yellow borer is solved, the survival rate and hatching rate of larvae are improved, the growth needs of yellow borer in each growth stage are met, and the breeding procedures are simplified.
Patent Information
- Application Number
- CN202510113670.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-24
- Publication Date
- 2025-04-25
AI Technical Summary
In the prior art, the indoor feeding effect of yellow borer is poor, especially the survival rate of larvae is low, which limits the process of yellow borer prevention and control research.
It provides a formula feed for raising yellow borer indoors, including fresh water chestnuts, sugar cane stem powder, agar powder, wheat germ powder, casein, yeast powder, sucrose, cholesterol, choline chloride, ascorbic acid, methylparaben, sorbic acid, multi-dimensional element tablets and formaldehyde, etc., which are prepared by step-by-step mixtures and are suitable for the entire growth stage of yellow borer.
The formula feed is low cost, easy to obtain raw materials, and simple preparation technology. It can significantly improve the survival rate and hatch rate of larvae of yellow borer, meet the growth needs of yellow borer at each growth stage, simplify the breeding procedures, and save manpower and material resources.
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Abstract
Description
[Technical field]
[0001] The invention relates to the technical field of breeding, and in particular to a formula feed for indoor breeding of yellow borer and a preparation method thereof. [Background technology]
[0002] The yellow borer is an important stem-boring pest of sugarcane. During the sugarcane seedling stage, the newly hatched larvae bore into the base of the sugarcane seedlings from the ground and feed on the seedlings. After damaging the growing point, the heart leaves of the sugarcane die, resulting in dead heart seedlings. In the middle and late stages of sugarcane growth, after the yellow borer larvae hatch, they mostly feed from the buds or root belts of the sugarcane stems and sneak into the sugarcane stems to cause damage, causing damage to the sugarcane buds and borer nodes, seriously affecting sugarcane seed retention and the quality and yield of sugarcane.
[0003] In the process of conducting prevention and control research and formulating management strategies for the yellow borer, indoor artificial breeding of the yellow borer can provide a stable insect source at a specific time and specific age for the comprehensive prevention and control of the yellow borer, such as drug resistance monitoring and mechanism research, drug screening, sex pheromones, transgenic insect-resistant sugarcane, parasitic wasps, etc. Therefore, the breeding and reproduction of the yellow borer is particularly important and will promote the research and prevention of the yellow borer. Based on this, Chinese patent CN 117546817 A discloses a method for breeding sugarcane yellow borer, and the specific breeding method includes using wild rice stem to breed the yellow borer. First, the wild rice stem needs to be replaced during the breeding process, and second, the larval survival rate under this method is not high.
[0004] Therefore, in the currently disclosed technology, the effect of raising yellow borer needs to be improved, which restricts the research progress of yellow borer prevention and control. Therefore, it is necessary to develop a more efficient yellow borer breeding method. [Summary of the invention]
[0005] In view of the above, it is necessary to provide a formula feed for indoor breeding of yellow borer and a breeding method for yellow borer. The formula feed for yellow borer has low cost, easy raw material acquisition, and simple preparation process. The feed can be used for indoor breeding and reproduction, laying the foundation for future indoor large-scale artificial breeding.
[0006] In order to achieve the above object, the technical solution adopted by the present invention is:
[0007] The invention discloses a formula feed for indoor feeding of yellow borer, comprising the following raw materials in parts by weight: 100-150 parts of fresh wild rice stem, 50-60 parts of sugarcane stem powder, 10-15 parts of agar powder, 30-40 parts of wheat germ powder, 5-10 parts of casein, 10-20 parts of yeast powder, 5-10 parts of sucrose, 0.15-0.25 parts of cholesterol, 0.4-0.5 parts of choline chloride, 1-1.5 parts of ascorbic acid, 1-1.3 parts of methyl paraben, 1-1.3 parts of sorbic acid, 0.5-1 parts of multi-dimensional element tablets and 0.2-0.5 parts of 40% formaldehyde.
[0008] Furthermore, the formula feed includes the following raw materials in parts by weight: 150 parts of fresh wild rice stem, 60 parts of sugarcane stem powder, 15 parts of agar powder, 40 parts of wheat germ powder, 10 parts of casein, 20 parts of yeast powder, 10 parts of sucrose, 0.25 parts of cholesterol, 0.5 parts of choline chloride, 1.5 parts of ascorbic acid, 1.3 parts of methyl paraben, 1.3 parts of sorbic acid, 1 part of multivitamin tablets and 0.5 parts of 40% formaldehyde.
[0009] The present application also provides a method for preparing the above-mentioned formula feed, which comprises the following steps:
[0010] (1) Preparation of sugarcane stem powder: peeling the sugarcane stems, slicing, roasting, and grinding into powder to obtain the sugarcane stem powder for later use;
[0011] (2) Preparation of wild rice stem pulp: Slice fresh wild rice stem and beat it into pulp to obtain wild rice stem pulp for later use;
[0012] (3) Preparation of a mixture: Cholesterol, choline chloride, ascorbic acid, methylparaben and sorbic acid are mixed, and then water is added thereto to dissolve the mixture, thereby obtaining a mixture for use;
[0013] (4) Preparation of mixed slurry: sugarcane stem powder, casein, wheat germ powder, yeast powder and sucrose are mixed, and then poured into the wild rice stem slurry obtained in step (2), distilled water is added and stirred evenly, and then heated to boiling to obtain a mixed slurry for use;
[0014] (5) Preparation of yellow borer feed: add distilled water to agar powder, then heat until the agar powder is completely melted, then pour into the mixed slurry obtained in step (4) and stir evenly, finally cool the material, add multidimensional element tablets and 40% formaldehyde, continue to stir evenly, cool and set, obtain the formula feed, and refrigerate at 4°C for use.
[0015] Furthermore, in the step (2), after slicing the water bamboo pulp and before beating it, the water bamboo slices are soaked in water at 45-50°C.
[0016] Furthermore, sodium chloride is added to the soaking water, and the mass ratio of the added sodium chloride to water is 1-2:100.
[0017] Furthermore, the temperature of water added in step (3) is 35-45°C.
[0018] Furthermore, in step (5), the material is cooled to 55-60° C. before adding the multidimensional element tablets and 40% formaldehyde.
[0019] Furthermore, the multidimensional element tablets are ground into powder before being added in step (5).
[0020] 40% formaldehyde is a formaldehyde aqueous solution with a volume concentration of 40%.
[0021] The present application also provides a method for raising yellow borer using the above-mentioned formula feed, comprising the following steps:
[0022] 1. Egg treatment: Put wet cotton balls into the spawning bottle with eggs, cover the bottle mouth with a double layer of paper towels and fix it with a rubber band, place a culture dish or culture dish lid larger than the diameter on top to cover the bottle mouth, so as to maintain a certain humidity in the bottle and prevent the eggs from drying out. When the eggs in the spawning bottle have black heads, take out the wet cotton balls, spray disinfectant (2% sodium hypochlorite and 2% formaldehyde by mass) on the inner wall of the spawning bottle for disinfection for about 5 minutes, then rinse with distilled water and let it dry for later use.
[0023] 2. Larvae feeding: Place the egg-laying bottle upside down on a multi-layer sterile paper towel, and place a culture dish with a smaller diameter than the egg-laying bottle on the paper towel. Put the prepared feed in the dish. When the eggs hatch into larvae the next day, the larvae will crawl into the feed to eat. After drilling into the feed, take it out and replace it with a new culture dish with feed to continue to let the larvae hatch and eat until the hatching is completed. Cover the taken out culture dish with multi-layer sterile filter paper, then cover the culture dish lid, and stack 5-7 culture dishes and tie them with rubber bands crosswise and place them on the insect raising rack, so that the larvae can feed themselves in the dish and pupate (most of them drill into the paper towel to pupate, and a few in the feed).
[0024] 3. Pupa treatment: After the larvae pupate, take the pupae or cocoons out of the culture dish, collect them and place them in a culture dish covered with 4 to 8 layers of wet sterile gauze and wet sterile cotton balls (this culture dish is smaller than the diameter of the egg-laying bottle), and put the culture dish into the egg-laying bottle. Cover the bottle mouth with a double layer of paper towels and fix it with a rubber band, and place a culture dish larger than the diameter on top to cover the bottle mouth to maintain a certain humidity in the bottle. Observe the moisture retention every day until the pupae emerge.
[0025] 4. Adult egg-laying: After the pupae in the egg-laying bottle emerge as adults, they mate freely and lay eggs on the inner wall of the bottle. During the egg-laying period, new egg-laying bottles should be replaced every day, and the gauze and cotton balls in the culture dish with pupae should be humidified every day to ensure that the pupae do not dry out and the adults absorb moisture until the adults finish laying eggs.
[0026] 5. Cycle feeding: Repeat steps 1 to 4 to continue feeding.
[0027] The present invention has at least the following beneficial effects:
[0028] 1. The yellow moth formula feed of the present application has low cost, easy raw material acquisition, simple preparation process, and fresh wild rice stem and sugarcane stem powder as main ingredients, combined with other nutrients required for growth. The materials are mixed in steps to finally obtain the yellow moth formula feed that can be used in the entire growth stage of the yellow moth. The feed can be used for indoor feeding and reproduction, laying the foundation for future indoor large-scale artificial breeding.
[0029] 2. The raw materials of the formulated feed for Tryporyza incertulas in this application include fresh water bamboo shoots. For the first time, it is proposed to make water bamboo shoots into formulated feed, which can attract Tryporyza incertulas to feed and improve the feeding enthusiasm of Tryporyza incertulas. In addition, before preparing the formulated feed, the water bamboo shoots are pretreated in this application. First, the water bamboo shoots are soaked in warm water containing sodium chloride, and then pulping treatment is carried out. Experiments have found that the formulated feed obtained by mixing the water bamboo shoot pulp after the above treatment with other components of the feed in this application can feed Tryporyza incertulas at different growth stages. If no pretreatment is carried out, there will be problems with the unsatisfactory survival rate of larvae. The applicant predicts that it may be due to the intake of components such as oxalate in the water bamboo shoots by the larvae, resulting in stress, death, etc. of the larvae. Therefore, soaking the water bamboo shoots in advance is carried out, and it is unexpectedly found that after slicing the water bamboo shoots and soaking them in a sodium chloride solution, the survival rate of the larvae can be effectively improved; the formulated feed prepared by the method in this application does not require formulating raw materials with different contents at different growth stages of Tryporyza incertulas. In the prior art, the feed generally needs to be fed in stages to avoid stress reactions in the animals being fed. In the early stage, components that are beneficial to digestion and absorption need to be fed, and the energy is increased in the later stage. However, the formulated feed combined in this application can solve the problem that the larvae are prone to stress reactions in the early stage, and at the same time meet the nutrient requirements of the adults in the later stage, streamline the breeding process, save manpower and material resources, and the obtained formulated feed has substantial significance.
[0030] 3. The present application also discloses a method for raising yellow borer by using the formula feed. In the raising method, the ovipositor is tied with a paper towel, and a culture dish or a culture dish cover is placed on it. The purpose is to keep a certain humidity in the bottle, and the paper towel also has a ventilating effect. The wet cotton ball is added to the bottle to keep the eggs in the bottle with a certain humidity, thereby improving the hatching rate. For larvae feeding, we use the ovipositor to stand on a multi-layer paper towel. The multi-layer paper towel has multiple uses. The first is to prevent the escape of the newly hatched larvae, the second is to be ventilated, and the third is to be easy to operate, and the paper towel is easy to buy. A culture dish with feed added is placed on the paper towel so that the larvae can crawl to the feed to feed after hatching. After drilling into the feed, the culture dish can be directly taken out, covered with paper and cover, and placed on the insect raising rack for feeding until pupation. The above operation solves the trouble of artificially connecting the larvae to the feed with a small brush for the newly hatched larvae, and the feed does not need to be changed during the larval stage, which saves labor and time, and reduces the pollution caused by artificial operation to the feed. This operation is innovative. When larvae are raised in a culture dish, the paper towel used under the cover is also very useful. It not only provides ventilation, moisture retention, and prevents larvae from escaping, but also provides a place for larvae to pupate. Most of the larvae will drill into the paper towel to pupate at the end of their instar, and very few will pupate in the feed. This operation also solves the problem of larvae being able to pupate smoothly, saves the tediousness of artificially taking out the pupation, saves labor and effort, and the multi-layer paper towel has a good pupation effect and improves the pupation rate. During the pupal emergence process, the pupa is directly placed in the culture dish, and the culture dish is then placed in the egg-laying bottle, so that it can mate freely and lay eggs on the inner wall of the bottle after emergence. No manual operation is required, and only the egg-laying bottle needs to be replaced every day. The purpose of the replacement is to make the eggs laid on the same day, so that the number of hatched larvae can be controlled when the feed is received later, and the incubation period is shortened, the feed replacement time is short, and it is easy to operate. [Specific implementation method]
[0031] In order to make the above-mentioned purpose, features and advantages of the present invention more obvious and easy to understand, the specific implementation of the present invention is described in detail below. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific implementation disclosed below.
[0032] Embodiment 1:
[0033] The present embodiment provides a formula feed for indoor feeding of yellow borer, the formula feed comprising the following raw materials in parts by weight: 100 parts of fresh wild rice stem, 50 parts of sugarcane stem powder, 10 parts of agar powder, 30 parts of wheat germ powder, 5 parts of casein, 10 parts of yeast powder, 5 parts of sucrose, 0.15 parts of cholesterol, 0.4 parts of choline chloride, 1 part of ascorbic acid, 1 part of methyl paraben, 1 part of sorbic acid, 0.5 parts of multivitamin tablets and 0.2 parts of 40% formaldehyde.
[0034] This embodiment also provides a method for preparing the above-mentioned formula feed, which comprises the following steps:
[0035] (1) Preparation of sugarcane stem powder: peeling the sugarcane stems, slicing, roasting, and grinding into powder to obtain the sugarcane stem powder for later use;
[0036] (2) Preparation of wild rice stem pulp: fresh wild rice stem slices are sliced and pulped to obtain wild rice stem pulp for later use; after slicing the wild rice stem pulp and before pulping, the wild rice stem slices are soaked in water at 45° C.; sodium chloride is added to the soaking water, and the mass ratio of the added sodium chloride to water is 1:100;
[0037] (3) Preparation of a mixture: Cholesterol, choline chloride, ascorbic acid, methylparaben and sorbic acid are mixed, and then 100 parts of water is added thereto to dissolve the mixture, and a mixture is obtained for use; the temperature of the added water is 35° C.;
[0038] (4) Preparation of mixed slurry: sugarcane stem powder, casein, wheat germ powder, yeast powder and sucrose are mixed, and then poured into the wild rice stem slurry obtained in step (2), 300 parts of distilled water are added and stirred evenly, and then heated to boiling to obtain a mixed slurry for use;
[0039] (5) Preparation of feed for yellow borer: 400 parts of distilled water are added to agar powder, and then heated until the agar powder is completely melted, and then the mixed slurry obtained in step (4) is poured into the mixture and stirred evenly, and then the material is cooled to 55° C., and multidimensional element tablets and 40% formaldehyde are added, and the mixture is stirred evenly, cooled and shaped to obtain the formula feed, which is refrigerated at 4° C. for use; before adding the multidimensional element tablets, it is ground into powder.
[0040] Embodiment 2:
[0041] The present embodiment provides a formula feed for indoor feeding of yellow borer, the formula feed comprising the following raw materials in parts by weight: 120 parts of fresh wild rice stem, 55 parts of sugarcane stem powder, 12 parts of agar powder, 35 parts of wheat germ powder, 7 parts of casein, 15 parts of yeast powder, 7 parts of sucrose, 0.2 parts of cholesterol, 0.4 parts of choline chloride, 1.2 parts of ascorbic acid, 1.1 parts of methyl paraben, 1.1 parts of sorbic acid, 0.7 parts of multivitamin tablets and 0.3 parts of 40% formaldehyde.
[0042] This embodiment also provides a method for preparing the above-mentioned formula feed, which comprises the following steps:
[0043] (1) Preparation of sugarcane stem powder: peeling the sugarcane stems, slicing, roasting, and grinding into powder to obtain the sugarcane stem powder for later use;
[0044] (2) Preparation of wild rice stem pulp: fresh wild rice stem slices are sliced and pulped to obtain wild rice stem pulp for later use; after slicing the wild rice stem pulp but before pulping, the wild rice stem slices are soaked in distilled water at 47° C.; sodium chloride is added to the distilled water for soaking, and the mass ratio of the added sodium chloride to the distilled water is 1:100;
[0045] (3) Preparation of a mixture: Cholesterol, choline chloride, ascorbic acid, methylparaben and sorbic acid are mixed, and then 100-200 parts of distilled water are added thereto to dissolve the mixture, and the mixture is prepared for use; the temperature of the added distilled water is 40° C.;
[0046] (4) Preparation of mixed slurry: sugarcane stem powder, casein, wheat germ powder, yeast powder and sucrose are mixed, and then poured into the wild rice stem slurry obtained in step (2), 350 parts of distilled water are added and stirred evenly, and then heated to boiling to obtain a mixed slurry for use;
[0047] (5) Preparation of yellow borer feed: add 350 parts of distilled water to agar powder, then heat until the agar powder is completely melted, then pour the mixed slurry obtained in step (4) into it and stir evenly, then cool the material to 58°C, add multidimensional element tablets and 40% formaldehyde, continue to stir evenly, cool and set, obtain the formula feed, and refrigerate at 4°C for use; grind it into powder before adding the multidimensional element tablets.
[0048] Embodiment 3:
[0049] The present embodiment provides a formula feed for indoor feeding of yellow borer, the formula feed comprising the following raw materials in parts by weight: 150 parts of fresh wild rice stem, 60 parts of sugarcane stem powder, 15 parts of agar powder, 40 parts of wheat germ powder, 10 parts of casein, 20 parts of yeast powder, 10 parts of sucrose, 0.25 parts of cholesterol, 0.5 parts of choline chloride, 1.5 parts of ascorbic acid, 1.3 parts of methyl paraben, 1.3 parts of sorbic acid, 1 part of multivitamin tablets and 0.5 parts of 40% formaldehyde.
[0050] This embodiment also provides a method for preparing the above-mentioned formula feed, which comprises the following steps:
[0051] (1) Preparation of sugarcane stem powder: peeling the sugarcane stems, slicing, roasting, and grinding into powder to obtain the sugarcane stem powder for later use;
[0052] (2) Preparation of wild rice stem pulp: fresh wild rice stem slices are sliced and beaten to obtain wild rice stem pulp for later use; after slicing the wild rice stem pulp but before beating it, the wild rice stem slices are soaked in distilled water at 50° C.; sodium chloride is added to the distilled water for soaking, and the mass ratio of the added sodium chloride to the distilled water is 2:100;
[0053] (3) Preparation of a mixture: Cholesterol, choline chloride, ascorbic acid, methylparaben and sorbic acid are mixed, and then 200 parts of distilled water are added thereto to dissolve the mixture, and a mixture is obtained for use; the temperature of the added distilled water is 45° C.;
[0054] (4) Preparation of mixed slurry: sugarcane stem powder, casein, wheat germ powder, yeast powder and sucrose are mixed, and then poured into the wild rice stem slurry obtained in step (2), 400 parts of distilled water are added and stirred evenly, and then heated to boiling to obtain a mixed slurry for use;
[0055] (5) Preparation of feed for yellow moth: add 300 parts of distilled water to agar powder, then heat until the agar powder is completely melted, then pour the mixed slurry obtained in step (4) into it and stir evenly, then cool the material to 60°C, add multidimensional element tablets and 40% formaldehyde, continue to stir evenly, cool and set, obtain the formula feed, and refrigerate at 4°C for use; grind it into powder before adding the multidimensional element tablets.
[0056] Embodiment 4:
[0057] This embodiment provides a method for indoor feeding of yellow borer using the yellow borer formula feed described in Example 1, the method comprising the following steps:
[0058] 1. Egg treatment: Put wet cotton balls into the spawning bottle with eggs, cover the bottle mouth with a double layer of paper towels and fix it with a rubber band, place a culture dish or culture dish lid larger than the diameter on top to cover the bottle mouth, so as to maintain a certain humidity in the bottle and prevent the eggs from drying out. When the eggs in the spawning bottle have black heads, take out the wet cotton balls, spray disinfectant (2% sodium hypochlorite and 2% formaldehyde by mass) on the inner wall of the spawning bottle for disinfection for about 5 minutes, then rinse with distilled water and let it dry for later use.
[0059] 2. Larvae feeding: Place the egg-laying bottle upside down on a multi-layer sterile paper towel, and place a culture dish with a smaller diameter than the egg-laying bottle on the paper towel. Put the prepared feed in the dish. When the eggs hatch into larvae the next day, the larvae will crawl into the feed to eat. After drilling into the feed, take it out and replace it with a new culture dish with feed to continue to let the larvae hatch and eat until the hatching is completed. Cover the taken out culture dish with multi-layer sterile filter paper, then cover the culture dish lid, and stack 5-7 culture dishes and tie them with rubber bands crosswise and place them on the insect raising rack, so that the larvae can feed themselves in the dish and pupate (most of them drill into the paper towel to pupate, and a few in the feed).
[0060] 3. Pupa treatment: After the larvae pupate, take the pupae or cocoons out of the culture dish, collect them and place them in a culture dish covered with 4 to 8 layers of wet sterile gauze and wet sterile cotton balls (this culture dish is smaller than the diameter of the egg-laying bottle), and put the culture dish into the egg-laying bottle. Cover the bottle mouth with a double layer of paper towels and fix it with a rubber band, and place a culture dish larger than the diameter on top to cover the bottle mouth to maintain a certain humidity in the bottle. Observe the moisture retention every day until the pupae emerge.
[0061] 4. Adult egg-laying: After the pupae in the egg-laying bottle emerge as adults, they mate freely and lay eggs on the inner wall of the bottle. During the egg-laying period, new egg-laying bottles should be replaced every day, and the gauze and cotton balls in the culture dish with pupae should be humidified every day to ensure that the pupae do not dry out and the adults absorb moisture until the adults finish laying eggs.
[0062] 5. Cycle feeding: Repeat steps 1-4 to continue feeding.
[0063] Embodiment 5:
[0064] This embodiment provides a method for raising yellow moth, using the formula feed of embodiment 2 as breeding feed, and other raising methods are the same as embodiment 4.
[0065] Embodiment 6:
[0066] This embodiment provides a method for raising yellow moth, using the formula feed of embodiment 3 as breeding feed, and other raising methods are the same as embodiment 4.
[0067] Comparative Example 1:
[0068] This comparative example provides a method for raising Sesamia sempervirens, in which fresh wild rice stem borer is used to raise the borer to the third age in the early stage, and then the feed according to the formula of Example 3 is used to raise the borer until it pupates.
[0069] Comparative Example 2:
[0070] This comparative example provides a method for feeding Sesamia sempervirens, wherein the fresh wild rice stem in the formula feed is replaced with fresh corn kernels, and the other methods are the same as those in Example 4.
[0071] Comparative Example 3:
[0072] This comparative example provides a method for raising yellow stem borer. The preparation of wild rice stem pulp is as follows: fresh wild rice stem is sliced and pulped to obtain wild rice stem pulp for standby use, that is, no soaking pretreatment is performed. Other methods are the same as those in Example 4.
[0073] Comparative Example 4:
[0074] This comparative example provides a method for raising yellow borer. The preparation of water bamboo pulp is as follows: after the water bamboo pulp is sliced and before it is pulped, the water bamboo slices are soaked in clean water at 50° C., and the other methods are the same as those in Example 4.
[0075] Comparative Example 5:
[0076] This comparative example provides a method for raising yellow stem borer, and the preparation of water bamboo pulp is as follows: fresh water bamboo is sliced and pulped to obtain water bamboo pulp for later use; after slicing the water bamboo pulp but before pulping, the water bamboo slices are soaked in 50°C water; the soaking water is water containing 8wt% citric acid, and the other methods are the same as those in Example 4.
[0077] Comparative Example 6:
[0078] Patent application number: 202311656349.6. Method for raising yellow borer.
[0079] Test example:
[0080] In order to illustrate the effect of the present application, this experiment compares the main life parameters of the yellow borer under different feeding methods, including the breeding methods of comparative example 6 and comparative examples 1-6. Except for the differences emphasized in each group, the other treatment methods are the same. The results are shown in Table 1:
[0081] Table 1 Main life parameters of Sesamina edulis under different feeding methods
[0082]
[0083]
[0084] According to the results in Table 1, the survival rate of the larvae of the yellow borer raised by the formula feed of the present application is above 70%, the hatching rate and the eclosion rate are both above 90%, the egg laying amount of a single female is greater than 300, and the pupa weight is heavier than the comparative example, indicating that the use of the formula feed of the present application to raise the yellow borer can meet the growth needs of the yellow borer at each growth stage, and does not need to be fed in stages, which improves the convenience of breeding and saves manpower and material resources. In the comparative example, if fresh corn kernels are used to replace wild rice stems, larval stress leads to death, which may be due to the insufficient proportion of fresh corn kernels in the formula feed, resulting in the larvae being unable to absorb and adapt. In addition, in the group where the fresh wild rice stems are not treated, the phenomenon of low larval survival rate also occurs, indicating that the method of pre-treating wild rice stems in the present application can improve the survival rate of larvae. Several embodiments of the present invention are described in more detail, but they cannot be understood as limiting the scope of the present invention. It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present invention, and these modifications and improvements all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the attached claims.
Claims
1. A formula feed for indoor rearing of yellow borer, characterized in that: The formula feed comprises the following raw materials in parts by weight: 100-150 parts of fresh wild rice stem, 50-60 parts of sugarcane stem powder, 10-15 parts of agar powder, 30-40 parts of wheat germ powder, 5-10 parts of casein, 10-20 parts of yeast powder, 5-10 parts of sucrose, 0.15-0.25 parts of cholesterol, 0.4-0.5 parts of choline chloride, 1-1.5 parts of ascorbic acid, 1-1.3 parts of methyl paraben, 1-1.3 parts of sorbic acid, 0.5-1 parts of multivitamin tablets and 0.2-0.5 parts of 40% formaldehyde.
2. The formula feed according to claim 1, characterized in that The formula feed comprises the following raw materials in parts by weight: 150 parts of fresh wild rice stem, 60 parts of sugarcane stem powder, 15 parts of agar powder, 40 parts of wheat germ powder, 10 parts of casein, 20 parts of yeast powder, 10 parts of sucrose, 0.25 parts of cholesterol, 0.5 parts of choline chloride, 1.5 parts of ascorbic acid, 1.3 parts of methyl paraben, 1.3 parts of sorbic acid, 1 part of multivitamin tablets and 0.5 parts of 40% formaldehyde.
3. The method for preparing a formula feed for indoor rearing of yellow borer according to claim 1, characterized in that: The method comprises the following steps: (1) Preparation of sugarcane stem powder: peeling the sugarcane stems, slicing, roasting, and grinding into powder to obtain the sugarcane stem powder for later use; (2) Preparation of wild rice stem pulp: Slice fresh wild rice stem and beat to obtain wild rice stem pulp for later use; (3) Preparation of a mixture: Cholesterol, choline chloride, ascorbic acid, methylparaben and sorbic acid are mixed, and then water is added thereto to dissolve the mixture, thereby obtaining a mixture for use; (4) Preparation of mixed slurry: sugarcane stem powder, casein, wheat germ powder, yeast powder and sucrose are mixed, and then poured into the wild rice stem slurry obtained in step (2), water is added and stirred evenly, and then heated to boiling to obtain a mixed slurry for later use; (5) Preparation of yellow borer feed: add water to agar powder, then heat until the agar powder is completely melted, then pour into the mixed slurry obtained in step (4) and stir evenly, finally cool the material, add multi-dimensional element tablets and 40% formaldehyde, continue to stir evenly, cool and set, obtain the formula feed, and refrigerate for later use.
4. The method according to claim 3, characterized in that In the step (2), after slicing the water bamboo pulp and before beating the water bamboo pulp, the water bamboo slices are soaked in water at 45-50°C.
5. The method according to claim 4, characterized in that Sodium chloride is added to the soaking water, and the mass ratio of the added sodium chloride to water is 1-2:
100.
6. The method according to claim 3, characterized in that The temperature of water added in step (3) is 35-45°C.
7. The method according to claim 3, characterized in that In the step (5), the material is cooled to 55-60° C. before adding the multidimensional element tablets and 40% formaldehyde.
8. The method according to claim 3, characterized in that In the step (5), the multidimensional element tablets are ground into powder before being added.
Citation Information
Patent Citations
Breeding method of yellow rice borers
CN117546817A