Composition with effects of dispelling effects of alcohol, protecting liver and protecting intestinal injury as well as preparation method and application of composition

Abstract

The invention belongs to the technical field of functional microorganisms, and particularly relates to a composition with effects of dispelling effects of alcohol, protecting liver and protecting intestinal injury as well as a preparation method and application of the composition. The composition with the effects of dispelling the effects of alcohol, protecting the liver and protecting the intestinal injury is prepared from the following components in parts by weight: 20 to 30 parts of lactobacillus delbrueckii powder, 20 to 30 parts of lactobacillus delbrueckii metabolite, 10 to 20 parts of lactobacillus plantarum powder and 10 to 20 parts of lactobacillus plantarum metabolite. The composition provided by the invention is environment-friendly, healthy and free from toxic and side effects and dependence. Through synergistic interaction between the lactobacillus delbrueckii powder and metabolites and the lactobacillus plantarum powder and metabolites, alcohol metabolism can be promoted, alcohol effect dispelling can be effectively achieved, the immunity of the organism can be enhanced, gastrointestinal tract and liver injury caused by alcohol is reduced, and the alcohol effect dispelling, liver protecting and intestinal injury protecting capacity of the product is further enhanced.

Description

Technical Field

[0001] The invention belongs to the technical field of functional microorganisms, and specifically relates to a composition with alcohol sobering, liver protecting and intestinal damage protecting functions, and a preparation method and application thereof. Background Art

[0002] Due to social and work needs, modern people's alcohol intake is gradually increasing. Intake of a large amount of alcohol in a short period of time will irritate the gastric mucosa, and in severe cases it will even cause gastric mucosal erosion and bleeding. In addition, alcohol is mainly metabolized by the liver. Drinking a lot of alcohol in a short period of time will not only increase the burden on the liver and cause liver damage, but also cause alcohol poisoning in severe cases, affecting the central nervous system, causing shock and coma in patients. Slow alcohol intake can cause intestinal microbiome disorders and intestinal barrier dysfunction in patients with alcoholic liver damage. In patients with severe alcohol poisoning, the central nervous system is deeply suppressed and they fall into a coma or even die.

[0003] There are many types of hangover products on the market, but most of them are chemical synthetic drugs, liver-protecting Chinese medicines or protein peptide drugs, which have problems such as slow effect, unstable efficacy, large side effects or unclear mechanism of action, and may even increase the burden on the liver; hangover drug formulas containing multiple Chinese medicines also have the problem of poor palatability. Developing natural and effective hangover and liver-protecting composite products is a new direction for the prevention and treatment of alcoholic liver disease, and is also a new direction for the research and development of hangover products today. Summary of the invention

[0004] Technical problem solved by the present invention: Most of the alcohol-detoxifying products currently on the market are chemically synthesized drugs, liver-protecting Chinese medicines or protein peptide drugs, which have the problems of slow effect, unstable efficacy, large side effects or unclear mechanism of action, and may even increase the burden on the liver; alcohol-detoxifying drug formulas containing multiple Chinese medicines also have the problem of poor palatability.

[0005] In view of the above technical problems, the present invention provides a composition having the effects of sobering up, protecting the liver and protecting the intestinal damage, and a preparation method and application thereof. The present invention provides a natural, healthy, effective and palatable composition formula having the effects of sobering up, protecting the liver and protecting the intestinal damage, and using live bacterial powder of Lactobacillus delbrueckii and Lactobacillus plantarum and their metabolites to prepare the composition, and the components synergize to achieve rapid sobering up, protect the gastrointestinal tract and liver of the human body, and prevent and treat alcoholic liver disease.

[0006] Specifically, the present invention provides the following technical solutions:

[0007] In a first aspect, the present invention provides a composition having the effects of sobering up, protecting the liver and protecting intestinal damage, which comprises, by weight, 20 to 30 parts of Lactobacillus delbrueckii powder, 20 to 30 parts of Lactobacillus delbrueckii metabolites, 10 to 20 parts of Lactobacillus plantarum powder and 10 to 20 parts of Lactobacillus plantarum metabolites.

[0008] Preferably, it comprises 25 to 29 parts by weight of Lactobacillus delbrueckii powder. And / or

[0009] Preferably, it comprises 25 to 29 parts by weight of Lactobacillus delbrueckii metabolites. And / or

[0010] Preferably, it comprises 15 to 20 parts by weight of Lactobacillus plantarum powder. And / or

[0011] Preferably, it comprises 15 to 20 parts by weight of Lactobacillus plantarum metabolites. And / or

[0012] Preferably, the Lactobacillus delbrueckii powder and / or Lactobacillus delbrueckii metabolite contains Lactobacillus delbrueckii, which is Lactobacillus delbrueckii subsp. lactis DangxiongLBⅧ, with a deposit number of CCTCC NO.: M2023396, deposited in China Center for Type Culture Collection. And / or

[0013] Preferably, the Lactobacillus plantarum powder and / or Lactobacillus plantarum metabolites contain Lactobacillus plantarum Ali.Plateau.LP.Ⅷ (Lactobacillus plantarum Ali.Plateau.LP.Ⅷ), with a deposit number of CCTCCNO: M2022068, which is deposited in China Center for Type Culture Collection. And / or

[0014] Preferably, the method for preparing the Lactobacillus delbrueckii powder comprises the following steps:

[0015] Step 1: inoculating Lactobacillus delbrueckii into a culture medium for activation;

[0016] Step 2: The activated Lactobacillus delbrueckii is inoculated into a culture medium for cultivation, the bacteria are separated and collected, and then a freeze-drying protective agent is added for freeze-drying to obtain Lactobacillus delbrueckii powder.

[0017] Preferably, in step 1, the inoculation amount of Lactobacillus delbrueckii is 10% to 15% based on the total volume of the culture medium; and / or, the culture medium used for activation is MRS culture medium; and / or, the activation temperature is 36 to 38°C; and / or, the activation time is 22 to 26 hours; and / or, the number of repeated passages is 2 to 3 times. and / or

[0018] Preferably, in step 2, the inoculation amount of Lactobacillus delbrueckii is 10% to 15% based on the total volume of the culture medium; and / or, the culture medium is MRS culture medium; and / or, the culture temperature is 35 to 39°C; and / or, the culture time is 24 to 48 hours; and / or, the separation speed is 5000 to 6500 rpmin, and / or, the separation time is 10 to 15 minutes. and / or

[0019] Preferably, in step 2, the weight ratio of the wet weight of the separated and collected bacterial cells to the lyophilization protective agent is 1:2-5. And / or

[0020] Preferably, the lyophilization protective agent comprises, by weight, 15 to 30 parts of skim milk, 5 to 15 parts of mannitol, 5 to 15 parts of trehalose, 1 to 5 parts of sodium glutamate and 1 to 5 parts of glycerol. And / or

[0021] Preferably, the viable count of Lactobacillus delbrueckii powder is 5.0×10^10 to 8.0×10^10 CFU / g. And / or

[0022] Preferably, the method for preparing the Lactobacillus delbrueckii metabolite comprises the following steps:

[0023] Step 1: inoculating Lactobacillus delbrueckii strains into a culture medium for cultivation, thereby obtaining a Lactobacillus delbrueckii seed solution;

[0024] Step 2: inoculate the Lactobacillus delbrueckii seed liquid into the fermentation medium for cultivation, inactivation, crushing and separation to obtain the supernatant, and concentrate and dry the supernatant to obtain the Lactobacillus delbrueckii metabolite.

[0025] Preferably, in step 1, the inoculation amount of Lactobacillus delbrueckii is 5% to 10% based on the total volume of the culture medium; and / or, the culture medium is MRS culture medium; and / or, the culture temperature is 35 to 39° C.; and / or, the culture time is 24 to 48 hours. and / or

[0026] Preferably, in step 2, the inoculation amount of the Lactobacillus delbrueckii seed solution is 10% to 15% based on the total volume of the culture medium. And / or

[0027] Preferably, in step 2, the culture conditions are: temperature of 35 to 39°C; and / or, the culture time is 24 to 48 hours. and / or

[0028] Preferably, in step 2, the fermentation medium comprises, by weight, 20-30 parts of glucose, 10-15 parts of brown sugar, 10-20 parts of skimmed milk powder, 6-12 parts of soy peptone, 4-8 parts of yeast extract powder, 0.1-0.3 parts of sodium chloride and 1000 parts of water. And / or

[0029] Preferably, in step 2, the inactivation conditions are: a temperature of 95°C to 105°C; and / or a time of 10 to 30 minutes. and / or

[0030] Preferably, in step 2, the separation conditions are: a rotation speed of 5000 to 6000 rpm; and / or a separation time of 5 to 10 min. and / or

[0031] Preferably, in step 2, the conditions for disruption are: ultrasonic power 200-400 W, working for 5-30 seconds and then resting for 5-30 seconds, and repeating this process until the bacteria are completely disrupted.

[0032] Preferably, in step 2, the concentration conditions are: temperature of 30-35°C; and / or time of 30-40 min. and / or

[0033] Preferably, in step 2, the drying is spray drying; preferably, the conditions of the spray drying are: the air inlet temperature is 155-160°C; and / or, the air outlet temperature is 80-85°C; and / or, the vacuum degree is 0.1Pa-0.00001Pa. and / or

[0034] Preferably, the method for preparing the Lactobacillus plantarum powder comprises the following steps:

[0035] Step 1: inoculating Lactobacillus plantarum into a culture medium for activation;

[0036] Step 2: The activated Lactobacillus plantarum is inoculated into a culture medium for cultivation, the bacteria are separated and collected, and then a freeze-drying protective agent is added for freeze-drying to obtain Lactobacillus plantarum powder.

[0037] Preferably, in step 1, the inoculum amount of Lactobacillus plantarum is 10% to 15% based on the total volume of the culture medium; and / or the culture medium used for activation is MRS culture medium; and / or the activation temperature is 36 to 38° C.; and / or the activation time is 22 to 26 h; and / or the number of passages is 2 to 3 times.

[0038] and / or

[0039] Preferably, in step 2, the inoculation amount of Lactobacillus plantarum is 10% to 15% based on the total volume of the culture medium; and / or, the culture medium is MRS culture medium; and / or, the culture temperature is 35 to 39° C.; and / or, the culture time is 12 to 24 hours; and / or, the separation speed is 5000 to 6500 rpm; and / or, the separation time is 10 to 15 minutes. and / or

[0040] Preferably, in step 2, the weight ratio of the wet weight of the separated and collected bacterial cells to the lyophilization protective agent is 1:2-5. And / or

[0041] Preferably, in step 2, the lyoprotectant comprises, by weight, 15 to 30 parts of skim milk, 5 to 15 parts of sorbitol, 5 to 15 parts of trehalose, 1 to 5 parts of sodium glutamate and 1 to 5 parts of glycerol. And / or

[0042] Preferably, the viable bacterial count of the Lactobacillus plantarum powder is 5.0×10^10 to 8.0×10^10 CFU / g. And / or

[0043] Preferably, the method for preparing the Lactobacillus plantarum metabolite comprises the following steps:

[0044] Step 1: inoculating Lactobacillus plantarum strains into a culture medium for cultivation, thereby obtaining Lactobacillus plantarum seed liquid;

[0045] Step 2: inoculate the Lactobacillus plantarum seed liquid into the fermentation medium for cultivation, inactivation, crushing and separation to obtain the supernatant, and concentrate and dry the supernatant to obtain the Lactobacillus plantarum metabolite.

[0046] Preferably, in step 1, the inoculation amount of Lactobacillus plantarum is 5% to 10% based on the total volume of the culture medium; and / or the culture medium is MRS culture medium; and / or the culture temperature is 35 to 39° C.; and / or the culture time is 12 to 24 hours. and / or

[0047] Preferably, in step 2, the inoculation amount of Lactobacillus plantarum seed solution is 10-15% based on the total volume of the culture medium. And / or

[0048] Preferably, in step 2, the culture conditions are: temperature of 35-39°C; and / or, the culture time is 12-24 hours. and / or

[0049] Preferably, in step 2, the fermentation medium comprises, by weight, 20-30 parts of glucose, 10-15 parts of brown sugar, 10-20 parts of whey protein powder, 6-12 parts of wheat peptone, 4-8 parts of yeast extract powder, 0.1-0.3 parts of sodium chloride and 1000 parts of water. And / or

[0050] Preferably, in step 2, the inactivation conditions are: a temperature of 95 to 105°C; and / or a time of 10 to 30 minutes. and / or

[0051] Preferably, in step 2, the conditions for disruption are: ultrasonic power 200-400W, working for 5-30s and then resting for 5-30s, and repeating this process until the bacteria are completely disrupted. And / or

[0052] Preferably, in step 2, the centrifugal conditions are: a rotation speed of 5000-6000 rpm; and / or a time of 5-10 min. and / or

[0053] Preferably, in step 2, the concentration conditions are: temperature of 30-35°C; and / or time of 30-40 min. and / or

[0054] Preferably, in step 2, the drying is spray drying; preferably, the conditions of the spray drying are: an air inlet temperature of 155-160°C; and / or an air outlet temperature of 80-85°C; and / or a vacuum degree of 0.1Pa-0.00001Pa.

[0055] In a second aspect, the present invention provides a method for preparing a composition having alcohol sobering, liver protecting and intestinal damage protecting effects, comprising the following steps:

[0056] The Lactobacillus delbrueckii powder, the Lactobacillus delbrueckii metabolite, the Lactobacillus plantarum powder and the Lactobacillus plantarum metabolite are mixed.

[0057] Preferably, a composition having the effects of sobering up, protecting the liver and protecting the intestine from damage is prepared by the above-mentioned method for preparing the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage.

[0058] In the third aspect, the present invention provides a composition with alcohol sobering, liver protecting and intestinal damage protective effects or a composition with alcohol sobering, liver protecting and intestinal damage protective effects prepared by the preparation method of the composition with alcohol sobering, liver protecting and intestinal damage protective effects, and its use in the preparation of beverages with one or more of the effects of sobering up, liver protecting, preventing or alleviating liver damage and preventing or alleviating intestinal damage.

[0059] In a fourth aspect, the present invention provides a composition with alcohol sobering, liver protecting and intestinal damage protecting effects or a composition with alcohol sobering, liver protecting and intestinal damage protecting effects prepared by the preparation method of the composition with alcohol sobering, liver protecting and intestinal damage protecting effects, and its use in the preparation of health foods having one or more of alcohol sobering, liver protecting, preventing or alleviating liver damage and preventing or alleviating intestinal damage effects.

[0060] In a fifth aspect, the present invention provides a composition with alcohol sobering, liver protecting and intestinal damage protective effects as mentioned above, or a composition with alcohol sobering, liver protecting and intestinal damage protective effects as prepared by the preparation method of the composition with alcohol sobering, liver protecting and intestinal damage protective effects as mentioned above, and its use in the preparation of a drug with one or more of alcohol sobering, liver protecting, preventing or alleviating liver damage and preventing or alleviating intestinal damage effects.

[0061] In a sixth aspect, the present invention provides the use of the above-mentioned composition with alcohol sobering, liver-protecting and intestinal damage protective effects or the composition with alcohol sobering, liver-protecting and intestinal damage protective effects prepared by the preparation method of the above-mentioned composition with alcohol sobering, liver-protecting and intestinal damage protective effects in the preparation of drugs for preventing and / or treating alcoholic liver disease.

[0062] In the seventh aspect, the present invention provides a beverage having one or more of the effects of sobering up, protecting the liver, preventing or alleviating liver damage, and preventing or alleviating intestinal damage, the beverage comprising the above-mentioned composition having the effects of sobering up, protecting the liver, and protecting from intestinal damage, or a composition having the effects of sobering up, protecting the liver, and protecting from intestinal damage prepared by the preparation method of the above-mentioned composition having the effects of sobering up, protecting the liver, and protecting from intestinal damage.

[0063] Preferably, based on the total weight of the beverage, the content of the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage is 0.5% to 5%.

[0064] In an eighth aspect, the present invention provides a health food having one or more of the effects of sobering up, protecting the liver, preventing or alleviating liver damage, and preventing or alleviating intestinal damage, the health food comprising the above-mentioned composition having the effects of sobering up, protecting the liver, and protecting from intestinal damage, or a composition having the effects of sobering up, protecting the liver, and protecting from intestinal damage prepared by the preparation method of the above-mentioned composition having the effects of sobering up, protecting the liver, and protecting from intestinal damage.

[0065] Preferably, based on the total weight of the health food, the content of the composition having the effects of sobering up, protecting the liver and protecting intestinal damage is 5% to 15%.

[0066] In a ninth aspect, the present invention provides a medicine having one or more of the effects of sobering up, protecting the liver, preventing or alleviating liver damage, and preventing or alleviating intestinal damage, the medicine comprising the above-mentioned composition having the effects of sobering up, protecting the liver, and protecting from intestinal damage, or a composition having the effects of sobering up, protecting the liver, and protecting from intestinal damage prepared by the preparation method of the above-mentioned composition having the effects of sobering up, protecting the liver, and protecting from intestinal damage.

[0067] Preferably, based on the total weight of the drug, the content of the composition having alcohol sobering, liver protecting and intestinal damage protecting effects is 5% to 15%.

[0068] In a tenth aspect, the present invention provides a drug for preventing and / or treating alcoholic liver disease, the drug comprising the above-mentioned composition having alcohol sobering, liver-protecting and intestinal damage protecting effects or a composition having alcohol sobering, liver-protecting and intestinal damage protecting effects prepared by the above-mentioned method for preparing the composition having alcohol sobering, liver-protecting and intestinal damage protecting effects.

[0069] Preferably, based on the total weight of the drug for preventing and / or treating alcoholic liver disease, the content of the composition having alcohol sobering, liver protecting and intestinal damage protecting effects is 10% to 30%.

[0070] Beneficial effects of the present invention

[0071] (1) The composition of the present invention having the effects of sobering up, protecting the liver and protecting the intestine from damage adopts the combination of bacterial powder and metabolites, which is environmentally friendly, healthy, non-toxic, non-addictive and has no side effects.

[0072] (2) In the composition of the present invention having the effects of sobering up, protecting the liver and protecting the intestine from damage, the bacterial powder and metabolites of Lactobacillus delbrueckii and the bacterial powder and metabolites of Lactobacillus plantarum have synergistic effects, which can not only promote alcohol metabolism and effectively sober up, but also enhance the body's immunity and reduce gastrointestinal and liver damage caused by alcohol, further enhancing the product's ability to sober up, protect the liver and protect the intestine from damage.

[0073] (3) The composition of the present invention having the effects of sobering up, protecting the liver and protecting the intestine from damage can be stored at room temperature and is not restricted in the scene of taking.

[0074] (4) The composition of the present invention having the effects of sobering up, protecting the liver and protecting the intestine from damage has a strong sobering ability and good palatability. It can inhibit the increase of aspartate aminotransferase to a certain extent, increase the tolerance of the animal body to alcohol and improve the liver damage caused by alcohol, and at the same time improve the intestinal structure and intestinal epithelial barrier damage caused by Escherichia coli infection. Whether it is a preventive administration of the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage or a therapeutic administration of the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage after infection, the integrity of the intestinal epithelial barrier can be maintained, and the protective effect of the preventive administration of the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage is better than that of the therapeutic administration of the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage.

[0075] Strain deposit information

[0076] Lactobacillus delbrueckii subsp. lactis Dangxiong LBⅧ was deposited in the China Center for Type Culture Collection on March 23, 2023, with a deposit number of CCTCCNO: M 2023396, and a deposit address of: Wuhan University, Wuhan, China, zip code: 430072; telephone: (027) 68754052. It is disclosed in the patent application number CN202310802241.7.

[0077] Lactobacillus plantarum Ali.Plateau.LP.VIII was deposited in China Center for Type Culture Collection on January 13, 2022, with a deposit number of CCTCC NO: M 2022068, deposit address: Wuhan University, Wuhan, China, postal code: 430072; telephone: (027) 68754052. Disclosed in patent application number 202210895628.7. BRIEF DESCRIPTION OF THE DRAWINGS

[0078] Figure 1 HE staining of the coronal section of mouse duodenum.

[0079] Figure 2 HE staining of the coronal section of mouse colon.

[0080] Figure 3 The graph shows the expression levels of tight junction proteins in the duodenum and colon of mice. DETAILED DESCRIPTION

[0081] As described above, the purpose of the present invention is to provide a composition having the effects of sobering up, protecting the liver and protecting intestinal damage, as well as a preparation method and application thereof.

[0082] In the present invention, the bacterial powder and the freeze-dried powder represent a substance, for example, the Lactobacillus delbrueckii powder and the Lactobacillus delbrueckii freeze-dried powder are a substance, the Lactobacillus plantarum powder and the Lactobacillus plantarum freeze-dried powder are a substance, the Lactobacillus delbrueckii CICC 23482 freeze-dried powder and the Lactobacillus delbrueckii CICC 23482 bacterial powder are a substance, and the Lactobacillus plantarum CICC 24936 freeze-dried powder and the Lactobacillus plantarum CICC 24936 bacterial powder are a substance.

[0083] In the present invention, the first purpose is to provide a composition with alcohol sobering, liver protecting and intestinal damage protecting effects, which comprises 20 to 30 parts of Lactobacillus delbrueckii powder, 20 to 30 parts of Lactobacillus delbrueckii metabolites, 10 to 20 parts of Lactobacillus plantarum powder and 10 to 20 parts of Lactobacillus plantarum metabolites in parts by weight.

[0084] Preferably, by weight, it comprises 25-29 parts of Lactobacillus delbrueckii powder, 25-29 parts of Lactobacillus delbrueckii metabolites, 15-20 parts of Lactobacillus plantarum powder and 15-20 parts of Lactobacillus plantarum metabolites. Within the preferred range, the composition has better effects of sobering up, protecting the liver and protecting intestinal damage.

[0085] Further preferably, the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage comprises 20 to 30 parts of Lactobacillus delbrueckii powder. The Lactobacillus delbrueckii powder is preferably Lactobacillus delbrueckii subspecies Dangxiong LBⅧ powder. For example, in some embodiments, the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage may contain 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 parts of Lactobacillus delbrueckii subspecies Dangxiong LBⅧ powder, or Lactobacillus delbrueckii subspecies Dangxiong LBⅧ powder within a numerical range consisting of any two of the above specific numerical values ​​as endpoints; and / or

[0086] Further preferably, the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage comprises 20 to 30 parts of Lactobacillus delbrueckii metabolites. The Lactobacillus delbrueckii metabolites are preferably metabolites of Lactobacillus delbrueckii subspecies Dangxiong LBⅧ. For example, in some embodiments, the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage may contain 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 parts of Lactobacillus delbrueckii subspecies Dangxiong LBⅧ metabolites, or metabolites of Lactobacillus delbrueckii subspecies Dangxiong LBⅧ within the numerical range formed by any two of the above specific numerical values ​​as endpoints.

[0087] Further preferably, the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage comprises 10 to 20 parts of Lactobacillus plantarum powder. The Lactobacillus plantarum powder is preferably Lactobacillus plantarum Ali.Plateau.LP.Ⅷ powder. For example, in some embodiments, the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage may contain 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 parts of Lactobacillus plantarum Ali.Plateau.LP.Ⅷ powder, or Lactobacillus plantarum Ali.Plateau.LP.Ⅷ powder within a numerical range consisting of any two of the above specific numerical values ​​as endpoints; and / or

[0088] Further preferably, the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage comprises 10 to 20 parts of Lactobacillus plantarum metabolites. The Lactobacillus plantarum metabolites are preferably Lactobacillus plantarum Ali.Plateau.LP.Ⅷ metabolites. For example, in some embodiments, the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage may contain 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 parts of Lactobacillus plantarum Ali.Plateau.LP.Ⅷ metabolites, or Lactobacillus plantarum Ali.Plateau.LP.Ⅷ metabolites within a numerical range consisting of any two of the above specific numerical values ​​as endpoints.

[0089] Preferably, the raw material for preparing the Lactobacillus delbrueckii powder and / or Lactobacillus delbrueckii metabolite contains Lactobacillus delbrueckii, and the Lactobacillus delbrueckii is Lactobacillus delbrueckii subsp. lactis Dangxiong LBⅧ, which was deposited in the China Center for Type Culture Collection on March 23, 2023, with a deposit number of CCTCC NO: M 2023396, and a deposit address: Wuhan University, Wuhan, China, Postal Code: 430072; Telephone: (027) 68754052. Among them, the Lactobacillus delbrueckii subsp. lactis Dangxiong LBⅧ was isolated from the milk of a herdsman's home at an altitude of about 4,350 meters in Dangxiong County, Tibet. It is a natural wild strain with good stress resistance and the ability to withstand strong adverse environments. Its isolation and identification process has been disclosed by Chinese Patent 202310802241.7.

[0090] Preferably, the raw material for preparing the Lactobacillus plantarum powder and / or Lactobacillus plantarum metabolites contains Lactobacillus plantarum, and the Lactobacillus plantarum is Lactobacillus plantarum Ali.Plateau.LP.VIII, which was deposited in the China Center for Type Culture Collection on January 13, 2022, with a deposit number of CCTCC NO: M 2022068, and a deposit address: Wuhan University, Wuhan, China, zip code: 430072; telephone: (027) 68754052. The Lactobacillus plantarum Ali.Plateau.LP.VIII was isolated from yak milk from herders' homes at an altitude of about 5,000 m in Ali, Tibet, and is a natural wild strain with food safety attributes. Its isolation and identification process has been disclosed by Chinese Patent 202210895628.7.

[0091] Preferably, in the present invention, the powder and metabolites of two bacteria, Lactobacillus delbrueckii and Lactobacillus plantarum, are used in combination to improve the antioxidant activity of the composition and produce substances with specific health benefits, such as conjugated linoleic acid (CLA). This substance has a variety of health benefits, such as improving blood lipid levels and reducing obesity; it can also play a positive role in promoting the absorption of nutrients and producing substances with specific health benefits, increasing the number of beneficial bacteria, and protecting intestinal health. More preferably, the composition with the effects of sobering up, protecting the liver and protecting intestinal damage contains the powder and metabolites of Lactobacillus delbrueckii subspecies Dangxiong LBⅧ and the powder and metabolites of Lactobacillus plantarum Ali.Plateau.LP.Ⅷ. The composition obtained by combining the powder and metabolites of the above two bacteria has a more excellent effect of sobering up, protecting the liver and protecting intestinal damage.

[0092] The second object of the present invention is to provide a method for preparing a composition having alcohol sobering, liver protecting and intestinal damage protecting effects, comprising the following steps: mixing Lactobacillus delbrueckii powder, Lactobacillus delbrueckii metabolites, Lactobacillus plantarum powder and Lactobacillus plantarum metabolites.

[0093] Preferably, the method for preparing the Lactobacillus delbrueckii powder comprises the following steps:

[0094] Step 1: inoculate Lactobacillus delbrueckii at a volume percentage of 10% to 15% in MRS culture medium for activation, and the activation conditions are: culture in a biochemical incubator at a temperature of 36 to 38° C. for 22 to 26 days, and repeat the above steps for 2 to 3 times;

[0095] Step 2: The activated Lactobacillus delbrueckii is inoculated into MRS medium at a volume percentage of 10% to 15%, cultured at a temperature of 35 to 39°C for 24 to 48 hours, and the bacteria are collected by centrifugation. The centrifugation conditions are: a speed of 5000 to 6500 rpm and a time of 10 to 15 minutes; then a freeze-drying protective agent is added, and the weight ratio of the separated bacteria wet weight to the freeze-drying protective agent is 1:2 to 5, and freeze-drying treatment is performed to obtain Lactobacillus delbrueckii powder. Wherein, the freeze-drying protective agent includes 15 to 30 parts of skim milk, 5 to 15 parts of mannitol, 5 to 15 parts of trehalose, 1 to 5 parts of sodium glutamate and 1 to 5 parts of glycerol. The number of viable bacteria of Lactobacillus delbrueckii powder is 5.0×10^10 to 8.0×10^10 CFU / g.

[0096] Preferably, the method for preparing the Lactobacillus delbrueckii metabolite comprises the following steps:

[0097] Step 1: inoculating Lactobacillus delbrueckii strains into MRS medium at an inoculum rate of 5% to 10% by volume for culturing at a temperature of 35 to 39° C. for 24 to 48 hours, thereby obtaining Lactobacillus delbrueckii seed solution;

[0098] Step 2: Inoculate the Lactobacillus delbrueckii seed liquid into the fermentation medium at an inoculum amount of 10% to 15% by volume, cultivate at a temperature of 35 to 39° C. for 24 to 48 hours, inactivate for 10 to 30 minutes at a temperature of 95 to 105° C., ultrasonic power of 200 to 400 W, work for 5 to 30 seconds and then leave for 5 to 30 seconds, repeat this process until the bacterial cell is completely broken, centrifuge at a speed of 5000 to 6000 rpm for 5 to 10 minutes, take the supernatant and concentrate at a temperature of 30 to 35° C. for 30 to 40 minutes; finally, spray dry, with an air inlet temperature of 155 to 160° C., an air outlet temperature of 80 to 85° C., and a vacuum degree of 0.1 to 0.00001 Pa, and dry to obtain the Lactobacillus delbrueckii metabolite. The fermentation medium comprises 20-30 parts of glucose, 10-15 parts of brown sugar, 10-20 parts of skimmed milk powder, 6-12 parts of soy peptone, 4-8 parts of yeast extract powder, 0.1-0.3 parts of sodium chloride and 1000 parts of water.

[0099] Preferably, the method for preparing the Lactobacillus plantarum powder comprises the following steps:

[0100] Step 1: Inoculate Lactobacillus plantarum at an inoculum volume percentage of 10% to 15% into MRS culture medium for activation. The activation conditions are: culture in a biochemical incubator at a temperature of 36 to 38° C. for 22 to 26 hours, and repeat the above steps for 2 to 3 times.

[0101] Step 2: The activated plant lactobacillus is inoculated into MRS medium at a volume percentage of 10% to 15% for cultivation, the culture conditions are: temperature of 35 to 39°C and time of 12 to 24 hours, centrifugal separation is performed to collect the bacteria, the centrifugal separation conditions are: speed of 5000 to 6500 rpm and time of 10 to 15 minutes; then a freeze-drying protective agent is added, the weight ratio of the bacteria wet weight obtained by centrifugal separation to the freeze-drying protective agent is 1:2 to 5, freeze-drying treatment is performed to obtain plant lactobacillus powder; wherein the freeze-drying protective agent includes 15 to 30 parts of skim milk, 5 to 15 parts of sorbitol, 5 to 15 parts of trehalose, 1 to 5 parts of sodium glutamate and 1 to 5 parts of glycerol. The viable bacterial count of the Lactobacillus delbrueckii powder is 5.0×10^10 to 8.0×10^10 CFU / g.

[0102] Preferably, the method for preparing the Lactobacillus plantarum metabolite comprises the following steps:

[0103] Step 1: inoculating Lactobacillus plantarum strains in an inoculum amount of 5% to 10% by volume into MRS medium for culturing at a temperature of 35 to 39° C. and a time of 12 to 24 hours to obtain Lactobacillus plantarum seed liquid;

[0104] Step 2: plant lactobacillus seed liquid is inoculated into the fermentation medium and cultivated at an inoculum size of 10% to 15% by volume, the temperature of cultivation is 35 to 39 ℃ and the time is 12 to 24h, deactivation is carried out under 95 to 105 ℃ of conditions for 10 to 30min, crushing ultrasonic power is 200 to 400W, work is carried out after 5 to 30s and an interval of 5 to 30s, repeat this process until the cell is broken completely, centrifugation, centrifugal rotating speed is 5000 to 6000rpm and the time is 5 to 15min, supernatant is obtained, and the temperature is 30 to 35 ℃ and concentrated for 30 to 40min, spray drying, air inlet temperature is 155 to 160 ℃, and air outlet temperature is 80 to 85 ℃ and vacuum is 0.1 to 0.00001Pa, namely obtains plant lactobacillus metabolite. The fermentation medium comprises 20-30 parts of glucose, 10-15 parts of brown sugar, 10-20 parts of whey protein powder, 6-12 parts of wheat peptone, 4-8 parts of yeast extract powder, 0.1-0.3 parts of sodium chloride and 1000 parts of water.

[0105] In the present invention, the wet weight of the bacteria refers to the direct weighing of the heavy phase obtained by centrifuging the solution after culturing Lactobacillus delbrueckii or Lactobacillus plantarum, which is the wet weight of the bacteria.

[0106] The third object of the present invention is to provide a composition having the effects of sobering up, protecting the liver and protecting the intestine from damage, and its use in the preparation of beverages, health products or medicines.

[0107] Preferably, the food has one or more of the following effects: sobering up, protecting the liver, preventing or alleviating liver damage, and preventing or alleviating intestinal damage.

[0108] Preferably, the health care product is a health care product having one or more of the effects of sobering up, protecting the liver, preventing or alleviating liver damage, and preventing or alleviating intestinal damage.

[0109] Preferably, the drug is a drug having one or more of the following effects: sobering up, protecting the liver, preventing or alleviating liver damage, and preventing or alleviating intestinal damage.

[0110] Preferably, the drug is a drug for preventing and / or treating alcoholic liver disease.

[0111] In the present invention, the fourth object is to provide a beverage having one or more of the effects of sobering up, protecting the liver, preventing or alleviating liver damage, and preventing or alleviating intestinal damage. The beverage comprises the above-mentioned composition having the effects of sobering up, protecting the liver, and protecting intestinal damage. The content of the composition having the effects of sobering up, protecting the liver, and protecting intestinal damage is 0.5% to 5% based on the total weight of the beverage.

[0112] In the present invention, the fifth object is to provide a health product having one or more of the effects of sobering up, protecting the liver, preventing or slowing down liver damage and preventing or slowing down intestinal damage. The health product comprises the above-mentioned protective composition having the effects of sobering up, protecting the liver and protecting intestinal damage. The content of the composition having the effects of sobering up, protecting the liver and protecting intestinal damage is 5% to 15% based on the total weight of the health food.

[0113] In the present invention, the sixth object is to provide a medicine having one or more of the effects of sobering up, protecting the liver, preventing or slowing down liver damage and preventing or slowing down intestinal damage. The medicine comprises the above-mentioned composition having the effects of sobering up, protecting the liver and protecting intestinal damage. The content of the composition having the effects of sobering up, protecting the liver and protecting intestinal damage is 5% to 15% based on the total weight of the medicine.

[0114] Alternatively, a drug for preventing and / or treating alcoholic liver disease comprises the above-mentioned composition having the effects of sobering up, protecting the liver and protecting intestinal damage, wherein the content of the composition having the effects of sobering up, protecting the liver and protecting intestinal damage is 10% to 30% based on the total weight of the drug for preventing and / or treating alcoholic liver disease.

[0115] The raw materials or reagents used in the present invention are purchased from mainstream manufacturers in the market. If the manufacturer or concentration is not specified, they are all analytically pure raw materials or reagents that can be routinely obtained. As long as they can play the expected role, there is no special restriction. The experimental methods without specific conditions are conventional methods and conventional conditions known in the field, or according to the conditions recommended by the instrument manufacturer. The experimental materials and instrument information used in the present invention are shown in Table 1 below.

[0116] Table 1 Experimental materials / instruments and manufacturers

[0117]

[0118] In order to better understand the technical solution of the present invention, the technical solution of the present invention is described in detail below in conjunction with specific embodiments.

[0119] Example 1

[0120] This embodiment provides a composition with alcohol sobering, liver protecting and intestinal damage protecting effects, comprising the following raw materials in the following weight proportions: 26 parts of Lactobacillus delbrueckii powder, 26 parts of Lactobacillus delbrueckii metabolites, 18 parts of Lactobacillus plantarum powder and 18 parts of Lactobacillus plantarum metabolites.

[0121] The Lactobacillus delbrueckii powder and the Lactobacillus delbrueckii metabolites contain Lactobacillus delbrueckii subspecies Dangxiong LBⅧ strain, with a preservation number of CCTCCNO: M 2023396. The Lactobacillus plantarum powder and the Lactobacillus plantarum metabolites contain Lactobacillus plantarum Ali. Plateau. LP.Ⅷ strain, with a preservation number of CCTCCNO: M 2022068.

[0122] Wherein, the preparation method of the Lactobacillus delbrueckii powder comprises the following steps:

[0123] Step 1: Lactobacillus delbrueckii subsp. lactis Dangxiong LBⅧ strain was inoculated into MRS medium at a volume percentage of 10% for activation. The activation conditions were: cultured in a biochemical incubator at a temperature of 37° C. for 24 hours, and the above steps were repeated for 2 times. The activation of the strain was performed in a clean bench.

[0124] Step 2: The activated Lactobacillus delbrueckii is inoculated into MRS medium at a volume percentage of 10% for cultivation. The culture conditions are: temperature of 37°C and time of 24h, centrifugal separation and collection of bacteria, centrifugal separation conditions: speed of 5000rpm and time of 15min; then add freeze-drying protective agent, the weight ratio of the wet weight of the bacteria to the freeze-drying protective agent is 1:3, freeze-drying treatment is performed to obtain Lactobacillus delbrueckii powder. Wherein, the freeze-drying protective agent includes 20 parts of skim milk, 13 parts of mannitol, 10 parts of trehalose, 2 parts of sodium glutamate and 2 parts of glycerol. The viable count of Lactobacillus delbrueckii powder is 6×10^10CFU / g.

[0125] Wherein, the preparation method of the Lactobacillus delbrueckii metabolite comprises the following steps:

[0126] Step 1: inoculating Lactobacillus delbrueckii subspecies Dangxiong LBⅧ strain into MRS medium at an inoculum volume percentage of 10% for culturing at a temperature of 37° C. and a culturing time of 24 h, thereby obtaining Lactobacillus delbrueckii seed liquid;

[0127] Step 2: Lactobacillus delbrueckii seed liquid is inoculated into the fermentation medium at an inoculum size of 10% by volume and cultivated, the temperature of cultivation is 37 ℃ and the time is 24h, the temperature is deactivated at 95 ℃ for 20min, the crushing ultrasonic power is 300W, the interval is 5-30s after working for 5-30s, and this process is repeated until the bacterial cell is completely crushed, centrifuged, the centrifugal speed is 5000rpm and the time is 10min, and the supernatant is obtained, and the temperature is concentrated for 30min at 30 ℃, spray-dried, the air inlet temperature is 158 ℃, the air outlet temperature is 80 ℃, and the vacuum degree in the spray-drying tower is 0.01MPa, and the Lactobacillus delbrueckii metabolite is obtained. Wherein, the fermentation medium comprises 20 parts of glucose, 10 parts of brown sugar, 10 parts of skimmed milk powder, 6 parts of soy peptone, 4 parts of yeast extract powder, 0.1 part of sodium chloride and 1000 parts of water.

[0128] Wherein, the preparation method of described plant lactobacillus powder comprises the following steps:

[0129] Step 1: Inoculate the plant lactobacillus Ali.Plateau.LP.Ⅷ strain at a volume percentage of 10% into MRS medium for activation. The activation conditions are: culture in an incubator at a temperature of 37° C. for 24 hours, and repeat the above steps for two times. The activation of the strain is performed in an ultra-clean workbench.

[0130] Step 2: The activated Lactobacillus plantarum is inoculated into MRS medium at a volume percentage of 10% for cultivation, the culture conditions are: temperature 37°C and time 12h, centrifugal separation is performed to collect the bacteria, the centrifugal separation conditions are: speed 5000rpm and time 15min; then a freeze-drying protective agent is added, the weight ratio of the bacteria wet weight obtained by centrifugation to the freeze-drying protective agent is 1:2, freeze-drying treatment is performed to obtain Lactobacillus plantarum powder; wherein the freeze-drying protective agent includes 20 parts of skim milk, 10 parts of sorbitol, 10 parts of trehalose, 3 parts of sodium glutamate and 2 parts of glycerol. The number of viable bacteria of the Lactobacillus plantarum powder is 8×10^10 CFU / g.

[0131] Wherein, the preparation method of the Lactobacillus plantarum metabolite comprises the following steps:

[0132] Step 1: Inoculate the plant lactobacillus strain into MRS medium at an inoculum volume percentage of 10% for culturing under the following conditions: temperature of 37° C. and time of 24 h, thereby obtaining plant lactobacillus seed liquid.

[0133] Step 2: plant lactobacillus seed liquid is inoculated into fermentation medium according to 10% inoculum volume percentage and cultivated, the temperature of cultivation is 37 ℃ and the time is 24h, inactivation is 15min under temperature 95 ℃ condition, the crushing ultrasonic power is 300W, 5~30s interval after working 5~30s, repeat this process until the cell is broken completely, centrifugal separation, centrifugal condition: rotating speed is 5500rpm and time is 10min, obtain supernatant, concentrate 30min under temperature 30 ℃ condition, adopt spray drying, spray drying condition: inlet air temperature is 158 ℃, outlet air temperature is 80 ℃ and vacuum degree in tower is 0.01MPa, namely obtain plant lactobacillus metabolite. Wherein, described fermentation medium comprises 20 parts of glucose, 10 parts of brown sugar, 10 parts of whey protein powder, 6 parts of wheat peptone, 4 parts of yeast extract powder, 0.1 part of sodium chloride and 1000 parts of water.

[0134] Example 2

[0135] The present embodiment provides a composition having the effects of sobering up, protecting the liver and protecting the intestine from damage, comprising the following raw materials in the following weight proportions: 30 parts of Lactobacillus delbrueckii powder, 30 parts of Lactobacillus delbrueckii metabolites, 13 parts of Lactobacillus plantarum powder and 13 parts of Lactobacillus plantarum metabolites.

[0136] The Lactobacillus delbrueckii powder and the Lactobacillus delbrueckii metabolites contain Lactobacillus delbrueckii subspecies Dangxiong LBⅧ strain, with a preservation number of CCTCCNO: M2023396. The Lactobacillus plantarum powder and the Lactobacillus plantarum metabolites contain Lactobacillus plantarum Ali. Plateau. LP.Ⅷ strain, with a preservation number of CCTCC NO: M2022068.

[0137] The preparation methods of the Lactobacillus delbrueckii powder, the Lactobacillus delbrueckii metabolites, the Lactobacillus plantarum powder and the Lactobacillus plantarum metabolites are the same as those in Example 1.

[0138] Example 3

[0139] This embodiment provides a composition with alcohol sobering, liver protecting and intestinal damage protecting effects, comprising the following raw materials in proportion by weight: 28 parts of Lactobacillus delbrueckii powder, 28 parts of Lactobacillus delbrueckii metabolites, 20 parts of Lactobacillus plantarum powder and 20 parts of Lactobacillus plantarum metabolites.

[0140] The Lactobacillus delbrueckii powder and the Lactobacillus delbrueckii metabolites contain Lactobacillus delbrueckii subspecies Dangxiong LBⅧ strain, with a preservation number of CCTCCNO: M2023396. The Lactobacillus plantarum powder and the Lactobacillus plantarum metabolites contain Lactobacillus plantarum Ali. Plateau. LP.Ⅷ strain, with a preservation number of CCTCC NO: M2022068.

[0141] The preparation methods of the Lactobacillus delbrueckii powder, the Lactobacillus delbrueckii metabolites, the Lactobacillus plantarum powder and the Lactobacillus plantarum metabolites are the same as those in Example 1.

[0142] Example 4

[0143] The present embodiment provides a composition having the effects of sobering up, protecting the liver and protecting the intestine from damage, comprising the following raw materials in proportion by weight: 20 parts of Lactobacillus delbrueckii powder, 20 parts of Lactobacillus delbrueckii metabolites, 10 parts of Lactobacillus plantarum powder and 10 parts of Lactobacillus plantarum metabolites.

[0144] The Lactobacillus delbrueckii powder and the Lactobacillus delbrueckii metabolites contain Lactobacillus delbrueckii subspecies Dangxiong LBⅧ strain, with a preservation number of CCTCCNO: M2023396. The Lactobacillus plantarum powder and the Lactobacillus plantarum metabolites contain Lactobacillus plantarum Ali. Plateau. LP.Ⅷ strain, with a preservation number of CCTCC NO: M2022068.

[0145] The preparation methods of the Lactobacillus delbrueckii powder, the Lactobacillus delbrueckii metabolites, the Lactobacillus plantarum powder and the Lactobacillus plantarum metabolites are the same as those in Example 1.

[0146] Example 5

[0147] The present embodiment provides a composition having the effects of sobering up, protecting the liver and protecting the intestine from damage, comprising the following raw materials in proportion by weight: 28 parts of freeze-dried powder of Lactobacillus delbrueckii CICC 23482, 28 parts of metabolites of Lactobacillus delbrueckii, 20 parts of Lactobacillus plantarum powder and 20 parts of Lactobacillus plantarum metabolites.

[0148] Among them, the Lactobacillus delbrueckii CICC 23482 freeze-dried powder was purchased from the China Industrial Microbiological Culture Collection Administration Center. The Lactobacillus delbrueckii contained in the Lactobacillus delbrueckii metabolite is the Lactobacillus delbrueckii lactis subspecies Dangxiong LBⅧ strain, with a preservation number of CCTCC NO: M2023396. The Lactobacillus plantarum contained in the Lactobacillus plantarum powder and the Lactobacillus plantarum metabolite is the Lactobacillus plantarum Ali.Plateau.LP.Ⅷ strain, with a preservation number of CCTCC NO: M2022068.

[0149] The preparation methods of the Lactobacillus delbrueckii metabolites, Lactobacillus plantarum powder and Lactobacillus plantarum metabolites are the same as those in Example 1.

[0150] Example 6

[0151] The present embodiment provides a composition having the effects of sobering up, protecting the liver and protecting the intestine from damage, comprising the following raw materials in proportion by weight: 28 parts of Lactobacillus delbrueckii powder, 28 parts of Lactobacillus delbrueckii metabolites, 20 parts of Lactobacillus plantarum CICC 24936 freeze-dried powder and 20 parts of Lactobacillus plantarum metabolites.

[0152] Among them, the plant lactobacillus CICC 24936 freeze-dried powder was purchased from the China Industrial Microbiological Culture Collection Administration Center. The Lactobacillus delbrueckii powder and the Lactobacillus delbrueckii metabolites contained Lactobacillus delbrueckii lactis subspecies Dangxiong LBⅧ strain, with a preservation number of CCTCC NO: M2023396. The plant lactobacillus plantarum contained in the plant lactobacillus plantarum metabolites was Lactobacillus plantarum Ali.Plateau.LP.Ⅷ strain, with a preservation number of CCTCC NO: M2022068.

[0153] The preparation methods of the Lactobacillus delbrueckii powder, the Lactobacillus delbrueckii metabolites and the Lactobacillus plantarum metabolites are the same as those in Example 1.

[0154] Comparative Example 1

[0155] The comparative example provides a composition with alcohol sobering, liver protecting and intestinal damage protecting effects, comprising the following raw materials in proportion by weight: 20 parts of Lactobacillus delbrueckii powder, 20 parts of Lactobacillus delbrueckii metabolites and 13 parts of Lactobacillus plantarum powder.

[0156] The Lactobacillus delbrueckii powder and the Lactobacillus delbrueckii metabolites contain Lactobacillus delbrueckii subspecies Dangxiong LBⅧ strain, with a preservation number of CCTCC NO: M2023396. The Lactobacillus plantarum powder contains Lactobacillus plantarum Ali. Plateau. LP.Ⅷ strain, with a preservation number of CCTCC NO: M2022068.

[0157] The preparation methods of the Lactobacillus delbrueckii powder, the Lactobacillus delbrueckii metabolites and the Lactobacillus plantarum powder are the same as those in Example 1.

[0158] Comparative Example 2

[0159] This comparative example provides a composition with alcohol sobering, liver protecting and intestinal damage protecting effects, comprising the following raw materials in proportion by weight: 28 parts of Lactobacillus delbrueckii freeze-dried powder and 28 parts of Lactobacillus delbrueckii metabolites.

[0160] Wherein, the Lactobacillus delbrueckii contained in the Lactobacillus delbrueckii powder and the Lactobacillus delbrueckii metabolites is the Lactobacillus delbrueckii subspecies Dangxiong LBⅧ strain, and its preservation number is CCTCCNO: M2023396.

[0161] The preparation methods of the Lactobacillus delbrueckii powder and the Lactobacillus delbrueckii metabolites are the same as those in Example 1.

[0162] Comparative Example 3

[0163] The comparative example provides a composition having the effects of sobering up, protecting the liver and protecting the intestinal damage, comprising the following raw materials in proportion by weight: 13 parts of Lactobacillus plantarum freeze-dried powder and 13 parts of Lactobacillus plantarum metabolites.

[0164] Wherein, the Lactobacillus plantarum powder and the Lactobacillus plantarum metabolites contain the Lactobacillus plantarum Ali. Plateau. LP. VIII strain, and the preservation number is CCTCC NO: M2022068.

[0165] The preparation methods of the Lactobacillus plantarum powder and the Lactobacillus plantarum metabolites are the same as those in Example 1.

[0166] Comparative Example 4

[0167] This embodiment provides a composition with alcohol sobering, liver protecting and intestinal damage protecting effects, comprising the following raw materials in proportion by weight: 18 parts of Lactobacillus delbrueckii powder, 18 parts of Lactobacillus delbrueckii metabolites, 9 parts of Lactobacillus plantarum powder and 9 parts of Lactobacillus plantarum metabolites.

[0168] The Lactobacillus delbrueckii powder and the Lactobacillus delbrueckii metabolites contain Lactobacillus delbrueckii subspecies Dangxiong LBⅧ strain, with a preservation number of CCTCCNO: M2023396. The Lactobacillus plantarum powder and the Lactobacillus plantarum metabolites contain Lactobacillus plantarum Ali. Plateau. LP.Ⅷ strain, with a preservation number of CCTCC NO: M2022068.

[0169] The preparation methods of the Lactobacillus delbrueckii powder, the Lactobacillus delbrueckii metabolites, the Lactobacillus plantarum powder and the Lactobacillus plantarum metabolites are the same as those in Example 1.

[0170] Application example 1: Determination of alcohol sobering ability

[0171] Fifty male mice were selected and randomly divided into five groups after adaptive feeding. After fasting (without water) overnight, the mice were gavaged with 0.1, 0.125, 0.15, 0.175 and 0.2 mL / 10 g BW of 56° Red Star Erguotou according to body weight. The righting reflex was used as an indicator of drunkenness. The specific operation was as follows: the mice were gently placed in the cage with their backs down. The mice could immediately right themselves and return to normal posture, indicating that the righting reflex existed. If they could not right themselves for more than 30 seconds, the righting reflex was considered to be lost, that is, they were judged to be in a drunken state. The spirit and activity of the mice were observed, the number of drunkenness and the number of deaths were recorded, and the dose with high drunkenness rate and survival rate but zero mortality was selected as the optimal dose.

[0172] According to the same standard, 96 mice were randomly divided into a blank control group, a model group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group and Comparative Example 4 group, with 8 mice in each group.

[0173] The compositions obtained in the examples and comparative examples with the effects of sobering up, protecting the liver and protecting the intestine from damage were prepared into a 25 mg / mL solution by adding sterile water. The mice in the blank control group and the model group were first given normal saline at a gavage volume of 20.0 mL / kg body weight; the mice in the Example 1 group were first given the composition with alcohol-relieving, liver-protecting and intestinal injury-protecting effects obtained in Example 1 at a gavage volume of 20.0 mL / kg body weight; the mice in the Example 2 group were first given the composition with alcohol-relieving, liver-protecting and intestinal injury-protecting effects obtained in Example 2 at a gavage volume of 20.0 mL / kg body weight; the mice in the Example 3 group were first given the composition with alcohol-relieving, liver-protecting and intestinal injury-protecting effects obtained in Example 3 at a gavage volume of 20.0 mL / kg body weight; the mice in the Example 4 group were first given the composition with alcohol-relieving, liver-protecting and intestinal injury-protecting effects obtained in Example 4 at a gavage volume of 20.0 mL / kg body weight; the mice in the Example 5 group were first given the composition with alcohol-relieving, liver-protecting and intestinal injury-protecting effects obtained in Example 5 at a gavage volume of 20.0 mL / kg body weight; the mice in the Example 6 group were first given the composition with alcohol-relieving, liver-protecting and intestinal injury-protecting effects obtained in Example 6 The mice in the comparative example 1 group were first given the composition with alcohol-relieving, liver-protecting and intestinal injury-protecting effects obtained in comparative example 1, and the gavage volume was 20.0 mL / kg body weight; the mice in the comparative example 2 group were first given the composition with alcohol-relieving, liver-protecting and intestinal injury-protecting effects obtained in comparative example 2, and the gavage volume was 20.0 mL / kg body weight; the mice in the comparative example 3 group were first given the composition with alcohol-relieving, liver-protecting and intestinal injury-protecting effects obtained in comparative example 3, and the gavage volume was 20.0 mL / kg body weight; the mice in the comparative example 4 group were first given the composition with alcohol-relieving, liver-protecting and intestinal injury-protecting effects obtained in comparative example 4, and the gavage volume was 20.0 mL / kg body weight; 30 minutes after gavage, the model group, the embodiment 1-6 groups and the comparative example 1-4 groups were respectively given the optimal dose (0.2 ml / 10 g BW dose) of Red Star Erguotou, and the righting reflex test was used to judge the drunkenness of the mice, and the righting reflex disappearance time during the incubation period of drunkenness and the number of animals awakening at 4h were recorded. The experimental results are shown in Table 2 below.

[0174] Table 2 Test data of average intoxication latency and number of awakenings

[0175]

[0176] Among them, the righting reflex disappearance time (also called average drunkenness latency) refers to the time from the start of alcohol intake to the disappearance of the righting reflex (the righting reflex disappearance means that the mouse is put into the mouse cage with its back down, and the mouse cannot immediately right its body position and resume normal posture within 30 seconds); the 4h animal awakening number refers to the number of mice whose righting reflex is recovered (the righting reflex is recovered means that the mouse is put into the mouse cage with its back down, and the mouse can right its body position and resume normal posture within 30 seconds) within 4 hours from the start of alcohol intake.

[0177] As can be seen from the above table, after each group of mice ingested sufficient alcohol, the average incubation period of drunkenness of the mice in the Example 1-6 group and the Comparative Example 4 group was higher than that in the model group, and the average incubation period of drunkenness of the mice in the Example 1-6 group was higher than that in the Comparative Example 4 group. After all the mice were drunk (i.e., in a drunken state where the righting reflex disappeared), the number of mice awakening within 4 hours was detected. There were 3 to 6 mice awakening in the Example 1-6 group, while the number of awakening mice in the Comparative Example 4 group was lower than that in the Example 1-6 group, and none of the drunken mice in the Comparative Example 1-3 group awakened.

[0178] Application Example 2: Determination of liver protection ability

[0179] The mice were killed 2 hours after intragastric alcohol administration, and liver and serum samples were taken for liver protection evaluation. The mouse liver tissue was weighed, and then a 10wt% liver homogenate was prepared, centrifuged at 3000rpm for 10min, and the supernatant was taken. The enzyme activity of aspartate aminotransferase, serum ADH activity and serum glutathione-peroxidase GSH-PX activity value were evaluated by ELISA. The experimental results are shown in Table 3 below.

[0180] Table 3 Hepatoprotective ability test data

[0181]

[0182]

[0183] The "activity unit" in the table means: at a temperature of 37°C, the amount of enzyme required (reduced) to catalyze the oxidation of 1 μmol of GSH in 1 min per 1L of serum is one GSH-PX activity unit. Among them, the enzyme activity of aspartate aminotransferase, serum ADH activity and serum GSH-PX activity were determined by ELISA kits. The ELISA kits were purchased from Shanghai Youkewei Biotechnology Co., Ltd.

[0184] It can be seen from Table 4 above:

[0185] Alcohol intake can cause mild enlargement of the liver and increase the liver weight. After intervention with the composition having the effects of sobering up, protecting the liver and protecting the intestine from injury, the liver weight of each group of the examples and comparative examples decreased relative to the model group. After intervention with the composition having the effects of sobering up, protecting the liver and protecting the intestine from injury, the liver weight of the groups of Examples 1-6 decreased significantly.

[0186] The enzyme activity of aspartate aminotransferase in the liver is increased after alcohol intervention, and can be partially restored by the intervention of the composition with alcohol sobering liver protection and intestinal damage protection functions of the examples and comparative examples. The composition with alcohol sobering liver protection and intestinal damage protection functions obtained in Examples 1-6 can significantly reduce the enzyme activity of aspartate aminotransferase to 214-342U / gprot., which shows that the composition with alcohol sobering liver protection and intestinal damage protection functions obtained in the examples inhibits the increase of aspartate aminotransferase enzyme activity to a certain extent, and can improve liver function damage caused by alcohol.

[0187] It can be seen in the serum samples that after consuming alcohol, the serum ADH activity in the model group was higher than that in the blank control group. After intervention with the composition with alcohol sobering, liver protecting and intestinal damage protection functions obtained in Examples 1-6, the serum ADH activity was significantly reduced. However, the composition with alcohol sobering, liver protecting and intestinal damage protection functions obtained in Comparative Examples 1-4 reduced the activity of serum ADH to a lesser extent. After consuming alcohol, the serum GSH-PX activity in the model group was lower than that in the blank control group. After intervention with the composition with alcohol sobering, liver protecting and intestinal damage protection functions obtained in Examples 1-6, the serum GSH-PX activity was significantly increased. However, the composition with alcohol sobering, liver protecting and intestinal damage protection functions obtained in Comparative Examples 1-3 increased the activity of serum GSH-PX to a lesser extent.

[0188] Application Example 3: Determination of intestinal damage protection ability

[0189] Mice aged 4-6 weeks were divided into 4 groups, 10 in each group, namely the control group, model group, prevention group and treatment group. The prevention group and the treatment group were intervened with the composition with alcohol sobering, liver protection and intestinal damage protection prepared in Example 3 and the effect was observed. Among them, the preparation method of Escherichia coli liquid includes the following steps: Escherichia coli lyophilized powder, with a preservation number of CICC 23657, is purchased from the China Industrial Microbiological Culture Collection Management Center, and is inoculated into MRS medium at a 10% inoculation amount based on the total volume of the culture medium, and cultured at 37°C for 24 hours to obtain Escherichia coli liquid (Escherichia coli liquid), and the number of viable bacteria is 1×10^8CFU / mL. The specific grouping and operation are shown in Table 4:

[0190] Table 4 Grouping and operation of strain protection injury intestinal infection model

[0191]

[0192] During the period, the weight and clinical manifestations of the mice were recorded. The mice were blooded from their orbits in the morning of the next day after the last administration of the composition, and were killed and the duodenum and colon were quickly collected. The intestinal tissue was observed by pathological histology by HE staining to evaluate the changes in intestinal structure. Specifically, step 1: dewaxing: Soak the intestinal tissue sections in xylene for 10 to 15 minutes to remove the paraffin on the sections. Step 2: hydration: Soak the sections in anhydrous ethanol, 95% ethanol, 80% ethanol and 70% ethanol for 5 minutes each to hydrate the sections. Step 3: staining: Stain the sections in hematoxylin stain for 5 to 10 minutes to stain the cell nuclei, rinse the sections with tap water to remove excess hematoxylin stain, and differentiate the sections in 1% hydrochloric acid ethanol for 30 seconds to darken the color of the cell nuclei. Rinse the sections with tap water to remove excess hydrochloric acid ethanol. Stain the sections in eosin stain for 2 to 5 minutes to stain the cytoplasm. Step 4: Dehydration: Soak the slices in 70% ethanol, 80% ethanol, 95% ethanol and anhydrous ethanol for 5 minutes each to dehydrate the slices. Step 5: Transparency: Soak the slices in xylene for 10 to 15 minutes to make the slices transparent. Step 6: Seal the slices with neutral gum to facilitate observation under a microscope; the specific HE staining images of the coronal section of the duodenum and the coronal section of the colon are shown in questions 1 and 2, where, Figure 1 and Figure 2 Each picture in the figure has three rows. The first row is the picture magnified 50 times, the second row is the picture magnified 100 times, and the third row is the picture magnified 200 times.

[0193] The composition obtained in Example 3 has the beneficial function of protecting the intestine. Whether the composition is administered therapeutically after infection or preventively, it can improve the intestinal villus structure and the integrity of the intestinal epithelial barrier.

[0194] exist Figure 1 Compared with the control group, the epithelial cells at the tips of the intestinal villi in the model group were significantly shed (black arrow → indicated), the villus thickness became thinner, the villus column was broken, and the goblet cells decreased; compared with the model group, the epithelial cells at the tips of the intestinal villi in the treatment group were improved (black round arrow The thickness and integrity of the villus column were improved; compared with the model group, the shedding of epithelial cells at the villus tip in the prevention group was improved to a certain extent, and the degree of improvement was better than that in the treatment group (black diamond dotted arrow However, compared with the normal group, there was still a certain amount of villous epithelial cell shedding, and the integrity of the villous column was also damaged to a certain extent, with scattered inflammatory cell infiltration (black diamond arrows) where indicated).

[0195] exist Figure 2In the control group, the colon mucosal surface was flat and smooth with parallel crypts, and the villus structure was intact without damage; compared with the control group, the colon tip of the model group was slightly swollen, inflammatory cells in the epithelium were visible (black arrow → pointed out), and the crypts were twisted and irregular; compared with the model group, the villus columns of the treatment group and the prevention group did not bend, the structure was relatively complete, and the crypts were relatively parallel.

[0196] The above results show that E. coli infection causes damage to the structure of the duodenum and colon and the intestinal epithelial barrier, among which the damage to the duodenum is more obvious in the intestinal villus structure. The composition of Example 3 embodies the beneficial function of protecting the intestine. Whether the composition is administered preventively or therapeutically after infection, the composition of Example 3 can improve the intestinal villus structure and the integrity of the intestinal epithelial barrier. The effect of protecting the intestinal epithelial barrier in the colon segment is better than that in the duodenum, and the protective effect of the preventive administration of the composition is better than that of the therapeutic administration of the composition.

[0197] The Western blot method was used (the principle is to use antigen-antibody reaction, transfer protein to the membrane by electrophoresis, and then use antibody for detection). The operation steps include: Step 1: protein sample preparation and determination; Step 2: protein extraction and denaturation; Step 3: SDS-PAGE electrophoresis; Step 4: membrane transfer; Step 5: immune reaction; Step 6: chemiluminescence and gel image analysis. The expression level changes of tight junction proteins Zo-1 and Claudin-1, key markers of mouse intestinal barrier, were detected. Among them, GAPDH was used as an internal reference and a reference gene.

[0198] Tight junction proteins Claudin-1 and Zo-1 are important functional proteins that ensure the integrity of the intestinal epithelial barrier, maintain normal intestinal permeability and intestinal barrier homeostasis. Under pathological conditions such as intestinal infection, the intestinal barrier is destroyed, which leads to the destruction of tight junction protein structure and changes in intestinal permeability. Figure 3 AB in the duodenal), E. coli infection severely damaged the expression of Zo-1 and Claudin 1 in the duodenum, causing barrier damage. The WB bands in the treatment group were darker in color, indicating that the administration of the composition to the treatment group could increase the expression of ZO-1 and Claudin 1. Figure 3 coli infection seriously damaged the expression of colon Zo-1 and Claudin 1, and the WB bands in the prevention group were darker in color, indicating that the administration of the composition to the prevention group could significantly increase the expression of ZO-1 and Claudin 1, while the treatment group that was administered the composition after infection had limited effect.

[0199] Although the above describes the specific implementation methods of the present invention, it does not limit the scope of protection of the present invention. Based on the technical solution of the present invention, various modifications or variations that can be made by those skilled in the art without creative work are still within the scope of protection of the present invention.

Claims

1. A composition having the effects of sobering up, protecting the liver and protecting the intestine from damage, characterized in that: The invention comprises, by weight, 20 to 30 parts of Lactobacillus delbrueckii powder, 20 to 30 parts of Lactobacillus delbrueckii metabolites, 10 to 20 parts of Lactobacillus plantarum powder and 10 to 20 parts of Lactobacillus plantarum metabolites.

2. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to claim 1, wherein: In parts by weight, it comprises 25 to 29 parts of Lactobacillus delbrueckii powder; and / or, in parts by weight, comprising 25 to 29 parts of a metabolite of Lactobacillus delbrueckii; And / or, in parts by weight, it comprises 15 to 20 parts of Lactobacillus plantarum powder; And / or, in parts by weight, it comprises 15 to 20 parts of Lactobacillus plantarum metabolites.

3. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to claim 1 or 2, wherein: The Lactobacillus delbrueckii powder and / or Lactobacillus delbrueckii metabolites contain Lactobacillus delbrueckii, which is Lactobacillus delbrueckii subsp. lactis Dangxiong LBⅧ, with a preservation number of CCTCCNO: M2023396, and is preserved in China Center for Type Culture Collection; And / or, the Lactobacillus plantarum powder and / or Lactobacillus plantarum metabolite contains Lactobacillus plantarum, which is Lactobacillus plantarum Ali. Plateau. LP. VIII, with a preservation number of CCTCC NO: M2022068, and is preserved in China Center for Type Culture Collection.

4. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to any one of claims 1 to 3, wherein: The preparation method of the Lactobacillus delbrueckii powder comprises the following steps: Step 1: inoculating Lactobacillus delbrueckii into a culture medium for activation; Step 2: The activated Lactobacillus delbrueckii is inoculated into a culture medium for cultivation, the bacteria are separated and collected, and then a freeze-drying protective agent is added for freeze-drying to obtain Lactobacillus delbrueckii powder.

5. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to claim 4, wherein: In step 1, the inoculation amount of Lactobacillus delbrueckii is 10% to 15% based on the total volume of the culture medium; and / or, the culture medium used for activation is MRS culture medium; and / or, the activation temperature is 36 to 38° C.; and / or, the activation time is 22 to 26 hours; and / or, the number of repeated passages is 2 to 3 times; And / or, in step 2, the inoculation amount of Lactobacillus delbrueckii is 10% to 15% based on the total volume of the culture medium; and / or, the culture medium is MRS culture medium; and / or, the culture temperature is 35 to 39° C.; and / or, the culture time is 24 to 48 hours; and / or, the separation speed is 5000 to 6500 rpm, and / or, the separation time is 10 to 15 minutes; And / or, in step 2, the weight ratio of the wet weight of the separated and collected bacterial cells to the lyophilization protective agent is 1:2-5.

6. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to claim 4 or 5, wherein: In parts by weight, the lyophilization protective agent includes 15 to 30 parts of skim milk, 5 to 15 parts of mannitol, 5 to 15 parts of trehalose, 1 to 5 parts of sodium glutamate and 1 to 5 parts of glycerol; Preferably, the viable count of Lactobacillus delbrueckii powder is 5.0×10^10 to 8.0×10^10 CFU / g.

7. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to any one of claims 1 to 6, wherein: The preparation method of the Lactobacillus delbrueckii metabolite comprises the following steps: Step 1: inoculating Lactobacillus delbrueckii strains into a culture medium for cultivation, thereby obtaining a Lactobacillus delbrueckii seed solution; Step 2: inoculate the Lactobacillus delbrueckii seed liquid into the fermentation medium for cultivation, inactivation, crushing and separation to obtain the supernatant, and concentrate and dry the supernatant to obtain the Lactobacillus delbrueckii metabolite.

8. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to claim 7, wherein: In step 1, the inoculation amount of Lactobacillus delbrueckii is 5% to 10% based on the total volume of the culture medium; And / or, the culture medium is MRS culture medium; and / or, the culture temperature is 35-39°C; And / or, the culture time is 24 to 48 hours.

9. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to claim 7 or 8, wherein: In step 2, the volume percentage of the inoculation amount of the Lactobacillus delbrueckii seed solution is 10% to 15% based on the total volume of the culture medium; and / or, the culture conditions are: a temperature of 35 to 39°C; and / or, a culture time of 24 to 48 hours; And / or, in parts by weight, the fermentation medium comprises 20-30 parts of glucose, 10-15 parts of brown sugar, 10-20 parts of skimmed milk powder, 6-12 parts of soy peptone, 4-8 parts of yeast extract powder, 0.1-0.3 parts of sodium chloride and 1000 parts of water; And / or, inactivation conditions: temperature is 95-105°C; and / or, time is 10-30 min; and / or, separation conditions: a rotation speed of 5000 to 6000 rpm; and / or, a time of 5 to 10 min; And / or, the conditions for disruption: ultrasonic power of 200-400W, until the bacteria are completely disrupted; And / or, the concentration conditions are: temperature of 30-35°C; and / or, time of 30-40 min; And / or, the drying is spray drying; preferably, the conditions of the spray drying are: the inlet air temperature is 155-160°C; and / or, the outlet air temperature is 80-85°C; and / or, the vacuum degree is 0.1Pa-0.00001Pa.

10. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to any one of claims 1 to 9, wherein: The preparation method of the plant lactobacillus powder comprises the following steps: Step 1: inoculating Lactobacillus plantarum into a culture medium for activation; Step 2: The activated Lactobacillus plantarum is inoculated into a culture medium for cultivation, the bacteria are separated and collected, and then a freeze-drying protective agent is added for freeze-drying to obtain Lactobacillus plantarum powder.

11. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to claim 10, wherein: In step 1, the volume percentage of the inoculum amount of Lactobacillus plantarum is 10% to 15% based on the total volume of the culture medium; and / or, the culture medium used for activation is MRS culture medium; and / or, the activation temperature is 36 to 38° C.; and / or, the activation time is 22 to 26 hours; and / or, the number of passages is 2 to 3 times; And / or, in step 2, the volume percentage of the inoculum amount of Lactobacillus plantarum is 10% to 15% based on the total volume of the culture medium; and / or, the culture medium is MRS culture medium; and / or, the culture temperature is 35 to 39° C.; and / or, the culture time is 12 to 24 hours; and / or, the separation speed is 5000 to 6500 rpm; and / or, the separation time is 10 to 15 minutes; And / or, in step 2, the weight ratio of the wet weight of the separated and collected bacterial cells to the lyophilization protective agent is 1:2-5.

12. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to claim 10 or 11, wherein: In step 2, the lyoprotectant comprises, by weight, 15 to 30 parts of skim milk, 5 to 15 parts of sorbitol, 5 to 15 parts of trehalose, 1 to 5 parts of sodium glutamate and 1 to 5 parts of glycerol; Preferably, the viable bacterial count of the Lactobacillus plantarum powder is 5.0×10^10 to 8.0×10^10 CFU / g.

13. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to any one of claims 1 to 12, wherein: The preparation method of the Lactobacillus plantarum metabolite comprises the following steps: Step 1: inoculating Lactobacillus plantarum strains into a culture medium for cultivation, thereby obtaining Lactobacillus plantarum seed liquid; Step 2: inoculate the Lactobacillus plantarum seed liquid into the fermentation medium for cultivation, inactivation, crushing and separation to obtain the supernatant, and concentrate and dry the supernatant to obtain the Lactobacillus plantarum metabolites.

14. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to claim 13, wherein: In step 1, the inoculation amount of Lactobacillus plantarum is 5% to 10% based on the total volume of the culture medium; And / or, the culture medium is MRS culture medium; and / or, the culture temperature is 35-39°C; And / or, the culture time is 12 to 24 hours.

15. The composition having alcohol sobering, liver protecting and intestinal damage protecting effects according to claim 13 or 14, wherein: In step 2, the inoculation amount of the Lactobacillus plantarum seed solution is 10% to 15% based on the total volume of the culture medium; and / or, the culture conditions are: a temperature of 35 to 39° C.; and / or, a culture time of 12 to 24 hours; And / or, in parts by weight, the fermentation medium comprises 20-30 parts of glucose, 10-15 parts of brown sugar, 10-20 parts of whey protein powder, 6-12 parts of wheat peptone, 4-8 parts of yeast extract powder, 0.1-0.3 parts of sodium chloride and 1000 parts of water; And / or, inactivation conditions: temperature is 95-105°C; and / or, time is 10-30 min; And / or, the conditions for disruption: ultrasonic power of 200-400W, until the bacteria are completely disrupted; and / or, centrifugal conditions: a rotation speed of 5000 to 6000 rpm; and / or, a time of 5 to 10 min; And / or, the concentration conditions are: temperature of 30-35°C; and / or, time of 30-40 min; And / or, the drying is spray drying; preferably, the conditions of the spray drying are: the inlet air temperature is 155-160°C; and / or, the outlet air temperature is 80-85°C; and / or, the vacuum degree is 0.1Pa-0.00001Pa.

16. A method for preparing a composition having alcohol sobering, liver protecting and intestinal damage protecting effects as claimed in any one of claims 1 to 15, characterized in that: The steps include: The Lactobacillus delbrueckii powder, the Lactobacillus delbrueckii metabolite, the Lactobacillus plantarum powder and the Lactobacillus plantarum metabolite are mixed.

17. Use of the composition with alcohol sobering, liver protection and intestinal damage protection effects according to any one of claims 1 to 15 or the composition with alcohol sobering, liver protection and intestinal damage protection effects prepared by the preparation method of the composition with alcohol sobering, liver protection and intestinal damage protection effects according to claim 16 in preparing a beverage with one or more of alcohol sobering, liver protection, liver damage prevention or alleviation and intestinal damage prevention or alleviation effects; And / or, use of the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage in the preparation of a health food having one or more of the effects of sobering up, protecting the liver, preventing or slowing down liver damage and preventing or slowing down intestinal damage; And / or, use of the composition having alcohol sobering, liver protecting and intestinal damage protecting effects in the preparation of a drug having one or more of alcohol sobering, liver protecting, preventing or alleviating liver damage and preventing or alleviating intestinal damage; And / or, use of the composition having alcohol sobering, liver protecting and intestinal damage protecting effects in the preparation of a drug for preventing and / or treating alcoholic liver disease.

18. A beverage having one or more of the following effects: hangover sobering effect, liver protection effect, prevention or alleviation of liver damage effect, and prevention or alleviation of intestinal damage effect, characterized in that: The beverage comprises the composition with alcohol sobering, liver protecting and intestinal damage protecting effects as described in any one of claims 1 to 15 or the composition with alcohol sobering, liver protecting and intestinal damage protecting effects prepared by the preparation method of the composition with alcohol sobering, liver protecting and intestinal damage protecting effects as described in claim 16; Preferably, based on the total weight of the beverage, the content of the composition having the effects of sobering up, protecting the liver and protecting the intestine from damage is 0.5% to 5%.

19. A health food having one or more of the following effects: hangover relief, liver protection, prevention or alleviation of liver damage, and prevention or alleviation of intestinal damage, characterized in that: The health food comprises the composition with alcohol sobering, liver protecting and intestinal injury protection effects as described in any one of claims 1 to 15 or the protective composition with alcohol sobering, liver protecting and intestinal injury protection effects prepared by the preparation method of the composition with alcohol sobering, liver protecting and intestinal injury protection effects as described in claim 16; Preferably, based on the total weight of the health food, the content of the composition having the effects of sobering up, protecting the liver and protecting intestinal damage is 5% to 15%.

20. A drug having one or more of the following effects: hangover sobering effect, liver protection effect, prevention or alleviation of liver damage effect, and prevention or alleviation of intestinal damage effect, characterized in that: The drug comprises the composition with alcohol sobering, liver protecting and intestinal damage protecting effects as described in any one of claims 1 to 15 or the composition with alcohol sobering, liver protecting and intestinal damage protecting effects prepared by the preparation method of the composition with alcohol sobering, liver protecting and intestinal damage protecting effects as described in claim 16; Preferably, based on the total weight of the drug, the content of the composition having alcohol sobering, liver protecting and intestinal damage protecting effects is 5% to 15%.

21. A drug for preventing and / or treating alcoholic liver disease, characterized in that: The drug comprises the composition with alcohol sobering, liver protecting and intestinal damage protecting effects as described in any one of claims 1 to 15 or the composition with alcohol sobering, liver protecting and intestinal damage protecting effects prepared by the preparation method of the composition with alcohol sobering, liver protecting and intestinal damage protecting effects as described in claim 16; Preferably, based on the total weight of the drug for preventing and / or treating alcoholic liver disease, the content of the composition having alcohol sobering, liver protecting and intestinal damage protecting effects is 10% to 30%.

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