Prebiotic composition and application thereof in preparation of functional food for dispelling effects of alcohol and protecting liver

Abstract

The invention relates to the technical field of functional food. The invention provides a prebiotic composition. The prebiotic composition comprises the following components: galactooligosaccharide, fructo-oligosaccharide, ampelopsis grossedentata leaves, aloe polysaccharide, hovenia dulcis thunb, flos puerariae, radix puerariae, corn oligopeptide, vitamins and sea cucumber peptide. The invention also provides an application of the prebiotic composition in preparation of functional food for dispelling the effects of alcohol and protecting liver, wherein the functional food for dispelling the effects of alcohol and protecting liver comprises various dosage forms of products such as functional oral liquid for dispelling the effects of alcohol and protecting liver. The functional food for dispelling the effects of alcohol and protecting the liver, which is prepared by utilizing the prebiotic composition, has good effects of dispelling the effects of alcohol, inducing resuscitation, protecting the liver, relieving uncomfortable symptoms of a body after drinking and the like; the alcohol dehydrogenase activity can be effectively enhanced, the alcohol metabolism capability of a body is improved, the drunkenness time is delayed, and the application range is wide.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, in particular to a prebiotic composition and application thereof in preparing functional foods for sobering up and protecting the liver. Background Art

[0002] In recent years, with the rapid economic development and the improvement of living standards, the number of people consuming alcohol and the amount of alcohol consumed have increased year by year. Long-term excessive drinking can induce more than 60 acute and chronic diseases, including alcohol poisoning, abnormal liver nodules, alcoholic cardiomyopathy, alcoholic gastritis, alcoholic cirrhosis, liver fibrosis, etc., causing serious damage to the cardiovascular, liver and nervous systems.

[0003] The liver is the main detoxification organ in the body. It decomposes, inactivates and excretes many drugs, exogenous poisons and other toxic substances. The liver is the main organ for metabolizing alcohol. Long-term excessive drinking will damage or destroy liver cells, affecting liver metabolism, and alcohol or cytotoxic metabolite acetaldehyde will accumulate, aggravating liver damage and creating a vicious cycle.

[0004] Most existing liver protection products can only improve the liver's antioxidant capacity, but cannot act on the direct damage to the liver caused by alcohol and its metabolites, nor can they promote the decomposition of alcohol or relieve the symptoms of drunkenness. Therefore, it is of great significance to develop products that can sober up, relieve alcohol, and reduce and prevent liver damage caused by alcohol. Summary of the invention

[0005] The purpose of the present invention is to provide a prebiotic composition and its application in the preparation of functional food for sobering up and protecting the liver. Through the reasonable combination and combined use of the prebiotic composition, the prepared product can enhance the activity of alcohol dehydrogenase, promote the body's alcohol metabolism ability, reduce the ethanol concentration in the blood, thereby playing the role of sobering up and protecting the liver, and can also alleviate the discomfort symptoms after drunkenness.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides a prebiotic composition, comprising the following components in parts by weight: 0.5-30 parts of galacto-oligosaccharide, 5-70 parts of fructo-oligosaccharide, 0.1-20 parts of ampelopsis grossedentata leaves, 0.1-30 parts of aloe polysaccharide, 0.1-20 parts of Hovenia dulcis seeds, 0.2-16 parts of kudzu flower, 0.5-20 parts of kudzu root, 0.5-30 parts of corn oligopeptide, 0.5-20 parts of vitamins and 0.1-30 parts of sea cucumber peptide.

[0008] Preferably, the composition comprises the following components in parts by weight: 1-25 parts of galacto-oligosaccharide, 7-65 parts of fructo-oligosaccharide, 0.5-18 parts of Ampelopsis grossedentata leaves, 0.3-27 parts of aloe polysaccharide, 0.2-17 parts of Hovenia dulcis seeds, 0.5-15 parts of Pueraria flowers, 0.8-18 parts of Pueraria roots, 1-26 parts of corn oligopeptides, 0.7-18.5 parts of vitamins and 0.4-28 parts of sea cucumber peptides.

[0009] The present invention also provides application of the prebiotic composition in preparing functional food for sobering up and protecting liver.

[0010] Preferably, the mass percentage of the prebiotic composition in the alcohol sobering and liver protecting functional food is 15-40%.

[0011] Preferably, the dosage form of the alcohol sobering and liver protecting functional food includes powder, granules, tablets, capsules, oral liquid and gel.

[0012] The present invention also provides a functional oral liquid for sobering up and protecting the liver, comprising the following components in percentage by weight: 1-10% broccoli extract, 5-12% gynostemma pentaphyllum extract, 5-15% bupleurum extract, 2-10% mulberry extract, 0.5-5% honey, 0.01-2% sorbic acid, 0.05-3% β-cyclodextrin, 0.01-3% lecithin, 10-25% mannitol and 15-40% of the prebiotic composition according to claim 1 or 2.

[0013] By adopting the above technical solution, the present invention has the following beneficial effects:

[0014] 1. The rationally formulated prebiotic composition of the present invention can prevent the degradation of the sugar chain structure of intestinal glycoproteins, maintain intestinal integrity, stimulate the activity and growth of beneficial bacterial colonies in the intestines; effectively enhance the activity of alcohol dehydrogenase, promote the body's alcohol metabolism ability, delay the time of drunkenness; and alleviate the discomfort symptoms after drunkenness. Among them, the combination of aloe polysaccharide and oligofructose has a significant liver protective effect on alcoholic liver damage; the combination of Hovenia dulcis, Pueraria root and corn oligopeptides can better promote alcohol metabolism, reduce the concentration of ethanol in the blood, and reduce the damage of ethanol to liver tissue.

[0015] 2. The raw materials used in the technical solution of the present invention are medicinal and edible raw materials, which can promote alcohol metabolism and protect the liver while also supplementing the nutrients needed by the human body. DETAILED DESCRIPTION

[0016] The invention provides a prebiotic composition, comprising the following components: galacto-oligosaccharide, fructo-oligosaccharide, Ampelopsis grossedentata leaf, aloe polysaccharide, Hovenia dulcis fruit, Pueraria flower, Pueraria root, corn oligopeptide, vitamins and sea cucumber peptide.

[0017] In the present invention, the weight proportion of the galacto-oligosaccharide is preferably 0.5-30 parts, further preferably 1-25 parts, and further preferably 23 parts; the weight proportion of the fructo-oligosaccharide is preferably 5-70 parts, further preferably 7-65 parts, and further preferably 60 parts; the weight proportion of the Ampelopsis grossedentata leaves is preferably 0.1-20 parts, further preferably 0.5-18 parts, and further preferably 15 parts; the weight proportion of the aloe polysaccharide is preferably 0.1-30 parts, further preferably 0.3-27 parts, and further preferably 25 parts; the weight proportion of the Hovenia dulcis fruit is preferably 0.1-20 parts, further preferably 0.2-17 parts, and further preferably 15 parts; the weight portion of the kudzu flower is preferably 0.2-16 parts, further preferably 0.5-15 parts, and further preferably 13 parts; the weight portion of the kudzu root is preferably 0.5-20 parts, further preferably 0.8-18 parts, and further preferably 16 parts; the weight portion of the corn oligopeptide is preferably 0.5-30 parts, further preferably 1-26 parts, and further preferably 24 parts; the weight portion of the vitamin is preferably 0.5-20 parts, further preferably 0.7-18.5 parts, and further preferably 17 parts; the weight portion of the sea cucumber peptide is preferably 0.1-30 parts, further preferably 0.4-28 parts, and further preferably 26 parts.

[0018] In the present invention, the aloe polysaccharide is the main biologically active component of aloe gel, and has the effects of immunomodulation, anti-tumor, liver protection, anti-radiation, anti-oxidation, anti-aging and hypoglycemic. Aloe polysaccharide can significantly reduce the activity of ALT and AST in serum, increase the activity of liver SOD, reduce the content of liver MDA, and has a certain protective effect on liver damage. The combination of aloe polysaccharide and oligofructose has a more significant liver protective effect on alcoholic liver damage.

[0019] In the present invention, the leaves of Ampelopsis serrulata contain rich flavonoids dihydromyricetin, which has obvious liver protection and liver protection effects and inhibits the formation of fatty liver. Studies have found that the myricetin in Ampelopsis serrulata can significantly reduce the ALT, AST activity and T-BIL content in the serum of mice with acute liver injury model induced by carbon tetrachloride, D-galactosamine and naphthyl isothiocyanate, and reduce the degeneration and necrosis of liver tissue, thereby achieving the effect of protecting the liver, reducing enzymes and jaundice.

[0020] In the present invention, the Hovenia dulcis contains a large amount of glucose and organic acids, which can not only expand the blood volume of the human body, but also detoxify alcohol, so it has the effect of sobering up and calming the nerves; the large amount of glucose, sucrose, fructose, organic acids, inorganic salts, vitamins, etc. contained in it can also promote the production of body fluids and quench thirst, clear away heat and relieve restlessness, and can supplement nutrients to the human body and enhance the body's disease resistance; the glucose and organic acids contained in it can not only expand the blood volume of the human body, but also detoxify alcohol, so it has the effect of sobering up and calming the nerves.

[0021] In the present invention, the corn oligopeptide is produced by using corn protein powder as raw material through pulping, protease hydrolysis, separation, filtration, spray drying and other processes. It is a new resource product. Its special amino acid composition and structure make the corn oligopeptide have multiple biological activities, such as promoting alcohol metabolism, protecting alcoholic chemical liver damage, enhancing immunity, etc.

[0022] In the present invention, the kudzu flower is a Chinese medicinal material, which is sweet and flat in nature, has the effect of detoxifying alcohol, and can improve the discomfort symptoms such as headache and vomiting caused by alcohol poisoning. The kudzu flower contains rich saponins, isoflavones and other substances, which can increase the speed of alcohol oxidation and decomposition, protect the gastric mucosa and intestinal mucosa, reduce the body's absorption of alcohol, and thus play the role of detoxifying alcohol.

[0023] In the present invention, the puerarin in the pueraria root can effectively reduce the damage of alcohol to the liver, and achieve the effect of sobering up and protecting the liver. Pueraria root also contains a large amount of flavonoids, triterpenoid saponins, coumarins, sterols, volatile oils, alkaloids, and essential amino acids and inorganic salts that cannot be synthesized by the human body. The total flavonoids of pueraria root can increase the activity of alcohol dehydrogenase, accelerate alcohol metabolism, reduce alcohol absorption, and also protect the liver through anti-oxidation. The synergistic use of the present invention's Hovenia dulcis, pueraria root and corn oligopeptide can relieve symptoms after drinking, shorten the sobering time, and regulate chronic diseases caused by long-term drinking.

[0024] In the present invention, the sea cucumber peptide is an extract of sea cucumber, which contains sea cucumber polysaccharides, sea cucumber saponins and other bioactive substances, and has the effects of repairing damaged cells, enhancing immunity, and resisting fatigue and aging.

[0025] The present invention also provides the use of the prebiotic composition in preparing functional food for sobering up and protecting the liver.

[0026] In the present invention, the mass percentage of the prebiotic composition in the functional food for sobering up and protecting the liver is preferably 15-40%, further preferably 18-38%, and further preferably 35%.

[0027] In the present invention, the dosage forms of the functional food for sobering up and protecting the liver include powder, granules, tablets, capsules, oral liquids and gels.

[0028] The present invention also provides a functional oral liquid for sobering up and protecting the liver, comprising the following components: broccoli extract, gynostemma pentaphyllum extract, bupleurum extract, mulberry extract, honey, sorbic acid, beta-cyclodextrin, lecithin, mannitol and the prebiotic composition.

[0029] In the present invention, the broccoli extract contains rich sulforaphane, which can remove free radicals in the body, has strong antioxidant capacity, and reduces liver oxidative damage. The Gynostemma pentaphyllum extract in the present invention can well inhibit lipid peroxidation in the liver and plasma, reduce the content of lipid oxides in the liver, and can also increase the activity of superoxidase in free radical-damaged cells, and has antioxidant and free radical damage effects; it can also inhibit acute liver damage caused by carbon tetrachloride, and has good protective functions for symptoms such as vacuolar degeneration, inflammatory infiltration, and necrosis of liver cells. The Bupleurum root of the present invention has the effects of relieving fever, soothing the liver and relieving depression, and raising Yang Qi, and is mainly used to treat fever due to superficial symptoms, Shaoyang syndrome, liver depression and qi stagnation, qi deficiency and sinking, organ prolapse, and antipyretic and antimalarial. The effective ingredient bupleurosaponin in Bupleurum root has anti-inflammatory effects, and can also resist fatty liver, liver damage, promote bile secretion, reduce transaminase, excite intestinal smooth muscle, inhibit gastric acid secretion, resist ulcers, and inhibit trypsin. The mulberry extract of the present invention contains rich biologically active substances, such as vitamin C, flavonoids, proanthocyanidins, etc., which have multiple functions such as preventing oxidation, enhancing immunity, and lowering blood sugar. At the same time, mulberry extract can also accelerate the excretion of alcohol, protect the liver and wake up the brain. Mulberries are rich in selenium, which can indirectly promote liver detoxification and detoxification, and have a certain alleviating effect on symptoms such as dizziness, drowsiness or brain excitement caused by alcohol after drinking. Mulberries taste sour and sweet, which can indirectly promote digestion, improve gastrointestinal function, promote gastric acid secretion, stimulate gastrointestinal peristalsis, and are beneficial to the decomposition of alcohol and metabolites to a certain extent. In addition, mulberries contain a special nutrient called "mulberry anthocyanin", which has a significant effect of dissolving alcohol substances.

[0030] In the present invention, the mass percentage of the broccoli extract is preferably 1-10%, further preferably 2-9%, and further preferably 3-8%; the mass percentage of the Gynostemma pentaphyllum extract is preferably 5-12%, further preferably 6-11%, and further preferably 7-10%; the mass percentage of the Bupleurum extract is preferably 5-15%, further preferably 6-14%, and further preferably 8-13%; the mass percentage of the mulberry extract is preferably 2-10%, further preferably 3-9%, and further preferably 4-8%; the mass percentage of the honey is preferably 0.5-5%, further preferably 0.8-4.6%, and further preferably 1-4.3%; the mass percentage of the sorbic acid is preferably 1-2%. The amount is preferably 0.01-2%, further preferably 0.05-1.8%, further preferably 0.1-1.7%; the mass percentage of the β-cyclodextrin is preferably 0.05-3%, further preferably 0.08-2.9%, further preferably 0.1-2.8%; the mass percentage of the lecithin is preferably 0.01-3%, further preferably 0.05-2.9%, further preferably 0.1-2.8%; the mass percentage of mannitol is preferably 10-25%, further preferably 11-24%, further preferably 12-23%; the mass percentage of the prebiotic composition is preferably 15-40%, further preferably 16-38%, further preferably 18-36%.

[0031] In the present invention, the preparation process of the broccoli extract is as follows: (1) the broccoli is dried and crushed, sieved, and the sieve is collected to obtain broccoli powder; (2) the broccoli powder and the extraction solvent are mixed in a mass ratio of 1: (10-20), ultrasonically extracted, filtered, and vacuum dried to obtain the broccoli extract. In the present invention, the broccoli is preferably dried in an oven to constant weight, and the drying temperature is preferably 40-50°C, further preferably 42-48°C, and further preferably 45°C. After drying, the broccoli is crushed and sieved, and the mesh number of the sieve is preferably 70-90 mesh, further preferably 75-85 mesh, and further preferably 80 mesh. In the present invention, the extraction solvent is preferably sodium dodecyl sulfate, potassium laurate or ethyl acetate, and further preferably potassium laurate; the concentration of potassium laurate is preferably 0.05-1wt%, and further preferably 0.1wt%. The mass ratio of the broccoli powder and the extraction solvent of the present invention is preferably 1: (10-20), further preferably 1: (12-18), and further preferably 1: 16. The ultrasonic time of the present invention is preferably 30-60min, further preferably 35-50min, and further preferably 45min; the ultrasonic temperature is preferably 60-70°C, further preferably 62-68°C, and further preferably 65°C; the ultrasonic frequency is preferably 10-30kHz, further preferably 15-25kHz, and further preferably 20kHz. In the present invention, centrifugation is preferably performed after ultrasonic extraction, and the centrifugal time is preferably 10-20min, further preferably 12-18min, and further preferably 15min; the centrifugal speed is preferably 2500-3500rpm, further preferably 2800-3200rpm, and further preferably 3000rpm.

[0032] In the present invention, the Gynostemma pentaphyllum extract, Bupleurum chinense extract and Mulberry extract are all prepared by ethanol reflux extraction, and the preparation process is: Gynostemma pentaphyllum or Bupleurum chinense or Mulberry are cut into sections and dried, and then mixed with ethanol solution according to a mass volume ratio of 1g: (5-20)mL, extracted, concentrated and then dried to obtain Gynostemma pentaphyllum extract or Bupleurum chinense extract or Mulberry extract. In the present invention, Gynostemma pentaphyllum or Bupleurum chinense or Mulberry are cut into sections of 0.3-1cm, further preferably 0.4-0.8cm, and further preferably 0.5-0.6cm; the drying is performed until the moisture content is less than 10%. The mass volume ratio of the mixture of the gynostemma pentaphyllum segment, the bupleurum segment or the mulberry segment and the ethanol solution in the present invention is preferably 1g:(5-20)mL, further preferably 1g:(8-15)mL, further preferably 1g:10mL; the ethanol solution is preferably 35-70vt%, further preferably 40-60vt%, further preferably 55vt%. In the present invention, the number of extractions is preferably 1-3 times, further preferably 2 times; the extraction time is preferably 25-40min, further preferably 28-37min, further preferably 32min; the extraction temperature is preferably 50-85°C, further preferably 55-80°C, further preferably 70°C. In the present invention, the powder is pulverized after concentration and drying, and then sieved after pulverization, and the sieve material is collected to obtain a solid powder; the drying temperature is preferably 50-65°C, further preferably 55-63°C, and further preferably 58°C; the drying time is preferably 10-20min, further preferably 13-18min, and further preferably 16min; the mesh number of the sieve is preferably 120-180 mesh, further preferably 140-170 mesh, and further preferably 150 mesh.

[0033] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0034] The fructooligosaccharide and galacto-oligosaccharide described in the present invention are derived from Yunfu Xinjinshan Biotechnology Co., Ltd.

[0035] Example 1

[0036] A prebiotic composition comprises the following components: 23 parts of galacto-oligosaccharide, 60 parts of fructo-oligosaccharide, 15 parts of ampelopsis ternata leaves, 25 parts of aloe polysaccharide, 15 parts of Hovenia dulcis seeds, 13 parts of kudzu flowers, 16 parts of kudzu roots, 24 parts of corn oligopeptides, 17 parts of vitamins and 26 parts of sea cucumber peptides.

[0037] Example 2

[0038] A prebiotic composition comprises the following components: 0.5 parts of galacto-oligosaccharide, 5 parts of fructo-oligosaccharide, 20 parts of ampelopsis ternata leaves, 0.1 parts of aloe polysaccharide, 20 parts of Hovenia dulcis seeds, 16 parts of kudzu flowers, 20 parts of kudzu roots, 0.5 parts of corn oligopeptides, 0.5 parts of vitamins and 30 parts of sea cucumber peptides.

[0039] Example 3

[0040] A prebiotic composition comprises the following components: 30 parts of galacto-oligosaccharide, 70 parts of fructo-oligosaccharide, 0.1 parts of Ampelopsis grossedentata leaves, 30 parts of aloe polysaccharide, 0.1 parts of Hovenia dulcis seeds, 0.2 parts of Pueraria flowers, 0.5 parts of Pueraria roots, 30 parts of corn oligopeptides, 20 parts of vitamins and 0.1 parts of sea cucumber peptides.

[0041] Example 4

[0042] A functional oral liquid for sobering up and protecting the liver, the formula of which is calculated by mass percentage as follows:

[0043] 8% broccoli extract, 9% Jiaogulan extract, 13% Bupleurum extract, 5% mulberry extract, 4.3% honey, 1.7% sorbic acid, 2.4% β-cyclodextrin, 2.6% lecithin, 20% mannitol and 34% of the prebiotic composition described in Example 1.

[0044] Example 5

[0045] A functional oral liquid for sobering up and protecting the liver, the formula of which is calculated by mass percentage as follows:

[0046] 3.4% broccoli extract, 6% Jiaogulan extract, 8% Bupleurum extract, 10% mulberry extract, 0.8% honey, 1.8% sorbic acid, 2.1% β-cyclodextrin, 2.9% lecithin, 25% mannitol and 40% of the prebiotic composition described in Example 1.

[0047] Example 6

[0048] A functional oral liquid for sobering up and protecting the liver, the formula of which is calculated by mass percentage as follows:

[0049] 10% broccoli extract, 10% Jiaogulan extract, 12% Bupleurum extract, 8% mulberry extract, 5% honey, 1.2% sorbic acid, 3% β-cyclodextrin, 2.8% lecithin, 20% mannitol and 28% of the prebiotic composition described in Example 1.

[0050] Comparative Example 1

[0051] As in Example 4, only 34% of the "prebiotic composition described in Example 1" is replaced with 34% of "23 parts of galacto-oligosaccharides, 60 parts of fructo-oligosaccharides, 15 parts of Agave ulmoides leaves, 25 parts of aloe polysaccharides, 13 parts of kudzu flowers, 17 parts of vitamins and 26 parts of sea cucumber peptides".

[0052] Comparative Example 2

[0053] Same as Example 4, only 34% of the "prebiotic composition described in Example 1" was replaced with 34% of "23 parts of galacto-oligosaccharides, 15 parts of Agave ulmoides leaves, 15 parts of Hovenia dulcis seeds, 13 parts of Pueraria flowers, 16 parts of Pueraria roots, 24 parts of corn oligopeptides, 17 parts of vitamins and 26 parts of sea cucumber peptides".

[0054] Comparative Example 3

[0055] Same as Example 4, except that "34% of the prebiotic composition described in Example 1" is replaced by "1% of the prebiotic composition described in Example 1".

[0056] Test Example 1

[0057] Fifty Kunming SPF mice (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.) were selected, 20±2g / mouse, and randomly divided into 5 groups, 10 mice in each group: model group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group. Each group of mice was fed with standard feeding and free drinking water. After adaptive continuous feeding for 5 days, they were fasted for 8 hours. The model group was given distilled water at a standard of 0.2mL / 10g, and the Example 4 group and the Comparative Example 1-3 groups were given the corresponding functional oral liquid for alcohol detoxification and liver protection at a standard of 0.2mL / 10g. After 40 minutes, each group of mice was given a 50% ethanol solution at an amount of 15mL / kg.

[0058] (I) Drunk Experiment

[0059] After 15 minutes of alcohol infusion, the mice in each group were observed for drunkenness, and the disappearance of righting reflex and recovery time of the mice were recorded. The mice that staggered and had a tendency to sleep were gently placed on a smooth horizontal table with their backs facing down. Those that kept their backs facing down for more than 30 seconds were considered drunk. The specific experimental results are shown in Table 1.

[0060] Table 1 Intoxication of mice in different treatment groups

[0061]

[0062]

[0063] As can be seen from Table 1, administering the oral liquid prepared by Example 4 and Comparative Examples 1-3 before drinking can reduce the number of drunk mice. At the same time, administering the oral liquid prepared by Example 4 and Comparative Examples 1-3 can also significantly prolong the time from drinking to the disappearance of the righting reflex of mice, and significantly shorten the righting reflex recovery time. Among them, the oral liquid administered to Example 4 group has the most significant effect. (two)

[0065] After 16 hours of ethanol gavage, mice in each group were killed, and liver tissues from the same part were taken, weighed, and added with physiological saline at a ratio of 1:20 (W / V), homogenized in an ice bath, and centrifuged at 4°C / 3000rpm for 10 minutes to prepare liver homogenates. Malondialdehyde (MDA, purchased from), reduced glutathione (GSH), serum triglyceride (TG) content, and alcohol dehydrogenase (ADH) activity in liver tissues were determined. The results are shown in Table 2.

[0066] Malondialdehyde was determined by a malondialdehyde kit purchased from Nanjing Jiancheng Bioengineering Institute; serum triglycerides were determined by a serum triglyceride kit purchased from Shanghai ELISA Biotechnology; reduced glutathione was determined by a reduced glutathione kit purchased from Shanghai Yiyan Biotechnology Co., Ltd.; alcohol dehydrogenase was determined by an alcohol dehydrogenase kit purchased from Shanghai ELISA Biotechnology.

[0067] Table 2 MDA, GSH, TG and ADH in liver tissues of mice in different treatment groups

[0068] Group MDA (nmol / mg) GSH (mg / g) TG (nmol / L) ADH (U / mL) Model Group 3.25±0.80 18.14±0.56 1.63±0.24 46.34±0.52 Example 4 Group 1.72±0.33 23.09±0.27 1.25±0.46 57.19±0.34 Comparative Example 1 2.35±0.62 21.76±71 1.39±0.50 52.07±0.23 Comparative Example 2 2.51±0.55 21.34±0.85 1.47±0.34 49.61±0.82 Comparative Example 3 2.63±0.74 20.08±0.32 1.43±0.19 51.32±0.61

[0069] As can be seen from Table 2, taking the products prepared in Example 4 and Comparative Examples 1-3 can significantly reduce the MDA content in the liver tissue of mice and increase the GSH content in the liver tissue, inhibit the increase in serum TG content caused by ethanol, enhance ADH activity, and thus accelerate the decomposition and metabolism of ethanol.

[0070] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A prebiotic composition, characterized in that The invention comprises the following components in parts by weight: 0.5-30 parts of galacto-oligosaccharide, 5-70 parts of fructo-oligosaccharide, 0.1-20 parts of ampelopsis pilosula leaves, 0.1-30 parts of aloe polysaccharide, 0.1-20 parts of Hovenia dulcis seeds, 0.2-16 parts of kudzu flower, 0.5-20 parts of kudzu root, 0.5-30 parts of corn oligopeptide, 0.5-20 parts of vitamins and 0.1-30 parts of sea cucumber peptide.

2. The prebiotic composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 1-25 parts of galacto-oligosaccharide, 7-65 parts of fructo-oligosaccharide, 0.5-18 parts of ampelopsis ternata leaves, 0.3-27 parts of aloe polysaccharide, 0.2-17 parts of Hovenia dulcis seeds, 0.5-14 parts of kudzu flowers, 0.8-18 parts of kudzu roots, 1-26 parts of corn oligopeptides, 0.7-18.5 parts of vitamins and 0.4-28 parts of sea cucumber peptides.

3. Use of the prebiotic composition according to claim 1 or 2 in the preparation of functional foods for sobering up and protecting the liver.

4. The use according to claim 3, characterized in that: The mass percentage of the prebiotic composition in the functional food for sobering up and protecting liver is 15-40%.

5. The use according to claim 3, characterized in that: The dosage forms of the alcohol-resolving and liver-protecting functional food include powder, granules, tablets, capsules, oral liquids and gels.

6. A functional oral liquid for sobering up and protecting the liver, characterized in that: The invention comprises the following components in percentage by weight: 1-10% broccoli extract, 5-12% gynostemma pentaphyllum extract, 5-15% bupleurum extract, 2-10% mulberry extract, 0.5-5% honey, 0.01-2% sorbic acid, 0.05-3% beta-cyclodextrin, 0.01-3% lecithin, 10-25% mannitol and 15-40% of the prebiotic composition according to claim 1 or 2.

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