Infrared fluorescence camera focusing system capable of automatically coupling exciting light and focal plane

Abstract

The invention discloses an infrared fluorescence camera focusing system capable of automatically coupling exciting light and a focal plane, which is applied to the technical field of fluorescence imaging. Comprising a double-path excitation light source, a rebound laser fixing frame, a focusing ring and an infrared fluorescence camera, the double-path excitation light sources are arranged on two sides of the infrared fluorescence camera and are used for exciting a specific contrast agent to generate a fluorescence signal; the rebounding laser fixing frame is tightly attached to the focusing ring and used for fixing the double-path excitation light source, and the angle of the double-path excitation light source is changed along with rotation of the focusing ring. The focusing ring is arranged in front of the infrared fluorescence camera and used for adjusting the focal plane of the infrared fluorescence camera and controlling the angle of the double-path excitation light source to achieve coupling of the excitation light irradiation face and the imaging plane. In the imaging process, the function of automatic coupling of the exciting light irradiation surface and the imaging plane can be achieved, and the operation of the imaging process is simplified.

Description

Technical Field

[0001] The invention relates to the technical field of fluorescence imaging, and more particularly to an infrared fluorescence camera focusing system with automatic coupling of excitation light and focal plane. Background Art

[0002] Near-infrared fluorescence imaging technology can mark target tissues under the action of contrast agents. Due to its excellent spatiotemporal resolution, its application scenarios are becoming increasingly extensive. Currently, the existing fluorescence imaging systems on the market have low integration and integration. When the imaging position changes, the excitation light irradiation surface needs to be manually changed, which is time-consuming and labor-intensive in actual use and is not conducive to the application expansion and promotion of infrared fluorescence imaging systems. Therefore, how to provide an infrared fluorescence camera focusing system that automatically couples the excitation light and the focal plane is a problem that technicians in this field urgently need to solve. Summary of the invention

[0003] In view of this, the present invention provides an infrared fluorescence camera focusing system with automatic coupling of excitation light and focal plane. During the imaging process, the excitation light irradiation surface and the imaging plane are automatically coupled, thereby solving the problems existing in the prior art.

[0004] In order to achieve the above object, the present invention provides the following technical solutions:

[0005] The invention discloses an infrared fluorescence camera focusing system with automatic coupling of excitation light and focal plane, comprising a dual-path excitation light source, a reboundable laser fixing frame, a focusing ring and an infrared fluorescence camera; the dual-path excitation light source is arranged at both sides of the infrared fluorescence camera and is used for exciting a specific contrast agent to generate a fluorescence signal; the reboundable laser fixing frame is close to the focusing ring and is used for fixing the dual-path excitation light source, and the angle of the dual-path excitation light source is changed as the focusing ring rotates; the focusing ring is arranged in front of the infrared fluorescence camera and is used for adjusting the focal plane of the infrared fluorescence camera and controlling the angle of the dual-path excitation light source to realize coupling of the excitation light irradiation surface and the imaging plane.

[0006] Optionally, the focus ring is elliptical, and the range of the rotation angle θ is 0-90 degrees.

[0007] Optionally, the focus of the infrared fluorescence camera changes as the focus ring is rotated:

[0008] f(θ)=f max +(f max -f min )·g(θ)

[0009] Where θ is the rotation angle of the focus ring, f is the current focal length, and f max and f min are the maximum focal length and minimum focal length of the infrared fluorescence camera respectively, and g(θ) represents a nonlinear function that reflects the change of focal length with the rotation angle of the focus ring.

[0010] Optionally, the angle θ2 between the dual-path excitation light source and the fixed fulcrum changes with the rotation of the focusing ring:

[0011]

[0012] Where d is half of the distance between the two laser fixed points in the dual-path excitation light source, h is half of the length L of the tangent line at the center of the focusing ring ellipse, and e is the length of the infrared fluorescence camera.

[0013] Optionally, the length L of the tangent line at the center of the focus ring ellipse changes as the focus ring rotates:

[0014]

[0015] Wherein, a is half of the length of the major axis of the focusing ring ellipse, and b is half of the length of the minor axis of the focusing ring ellipse; when θ=0, L=2a; when θ=90, L=2b.

[0016] Optionally, if the excitation light irradiation surface is coupled to the imaging plane, then:

[0017]

[0018] The major axis length 2a and the minor axis length 2b of the focusing ring ellipse are obtained by calculation. Under this condition, the excitation light irradiation surface and the imaging plane are automatically coupled, and the imaging position is changed by adjusting the rotation angle θ of the focusing ring ellipse.

[0019] It can be seen from the above technical solution that, compared with the prior art, the present invention provides an infrared fluorescence camera focusing system with automatic coupling of excitation light and focal plane, which has the following beneficial effects: the present invention realizes the function of automatic coupling of the excitation light irradiation surface and the imaging plane during the imaging process by adjusting the rotation angle of the focusing ring, thereby simplifying the operation of the imaging process and solving the problem that when the imaging position changes, the excitation light irradiation surface needs to be manually changed, which is time-consuming and labor-intensive and inconvenient to operate in actual use. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0021] Figure 1 This is a schematic diagram of the structure of the infrared fluorescence camera focusing system of the present invention;

[0022] Figure 2 It is a front view of the focusing system of the infrared fluorescence camera of the present invention;

[0023] Figure 3 is a schematic diagram of a tangent line of the center of the focus ring ellipse in an embodiment of the present invention;

[0024] Figure 4 It is a left view of the focusing system of the infrared fluorescence camera of the present invention;

[0025] Figure 5 It is a simplified schematic diagram of a left view of a focusing system of an infrared fluorescence camera in an embodiment of the present invention;

[0026] Figure 6 Schematic diagram of the coordinate system equivalent model in the embodiment of the present invention;

[0027] In the figure: 1- dual-channel excitation light source, 2- reboundable laser fixing frame, 3- focusing ring. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] The embodiment of the present invention discloses an infrared fluorescence camera focusing system with automatic coupling of excitation light and focal plane, such as Figure 1 As shown, it includes a dual-path excitation light source 1, a reboundable laser fixing frame 2, a focusing ring 3 and an infrared fluorescence camera; the dual-path excitation light source 1 is arranged on both sides of the infrared fluorescence camera, and is used to excite a specific contrast agent to generate a fluorescence signal; the reboundable laser fixing frame 2 is close to the focusing ring 3, and is used to fix the dual-path excitation light source 1, and the angle of the dual-path excitation light source 1 changes with the rotation of the focusing ring 3; the focusing ring 3 is arranged in front of the infrared fluorescence camera, and is used to adjust the focal plane of the infrared fluorescence camera, and control the angle of the dual-path excitation light source 1 to achieve the coupling of the excitation light irradiation surface and the imaging plane.

[0030] Furthermore, the focus ring 3 is elliptical, and the range of the rotation angle θ is 0-90 degrees.

[0031] Furthermore, the focal length of the infrared fluorescence camera changes as the focus ring 3 rotates:

[0032] f(θ)=f max +(f max -f min )·g(θ)

[0033] Where θ is the rotation angle of the focus ring 3, f is the current focal length, and f max and f minare the maximum focal length and the minimum focal length of the infrared fluorescence camera respectively, and g(θ) represents a nonlinear function reflecting the change of the focal length with the rotation angle of the focus ring 3.

[0034] g(θ) may be an exponential function or a logarithmic function. In the embodiment of the present invention, the use of a logarithmic function involves:

[0035]

[0036] In the formula, θ max is the maximum rotation angle of the focus ring 3.

[0037] Furthermore, the angle θ2 between the dual-path excitation light source 1 and the fixed fulcrum changes as the focusing ring 3 rotates:

[0038]

[0039] Where d is half of the distance between the two laser fixed points in the dual-path excitation light source 1, h is half of the length L of the tangent line at the center of the ellipse of the focusing ring 3, and e is the length of the infrared fluorescence camera. Therefore, the angle θ2 of the dual-path excitation light source 1 depends on the length of the tangent line passing through the center of the ellipse.

[0040] Further, such as Figure 2 and Figure 3 As shown, when the elliptical focusing ring 3 is rotated, as the rotation angle changes, the length L of the tangent line at the center of the ellipse of the focusing ring 3 changes with the rotation of the focusing ring 3:

[0041]

[0042] Wherein, a is half of the length of the major axis of the ellipse of the focusing ring 3, and b is half of the length of the minor axis of the ellipse of the focusing ring 3; when θ=0, L=2a; when θ=90, L=2b.

[0043] In the embodiment of the present invention, the derivation process of L(θ) is specifically as follows:

[0044] Let the center of the ellipse be the origin of the coordinate system and consider the equation of the ellipse:

[0045]

[0046] The length of the tangent line at the center of the ellipse of the focusing ring 3 is the straight line at the origin of the ellipse:

[0047] y=tan(θ)x

[0048] Substituting the equation of the line into the equation of the ellipse, we obtain:

[0049]

[0050] After finishing, we get:

[0051]

[0052] make:

[0053]

[0054] but:

[0055]

[0056] The corresponding y coordinate is:

[0057]

[0058] Therefore, the intersection point of the line and the ellipse is:

[0059]

[0060] The length of the ellipse's intangent is the distance between the two intersection points:

[0061]

[0062] The distance between two points is:

[0063]

[0064] Substitute tan 2 (θ)+1=sec 2 (θ), we can get:

[0065]

[0066] Further sorting gives:

[0067]

[0068] Furthermore, if the excitation light irradiation surface is coupled with the imaging plane, then:

[0069]

[0070] The major axis length 2a and the minor axis length 2b of the focus ring 3 are obtained by calculation. Under this condition, the excitation light irradiation surface and the imaging plane are automatically coupled, and the imaging position is changed by adjusting the rotation angle θ of the focus ring 3 .

[0071] In the embodiment of the present invention, the derivation process of the coupling between the excitation light irradiation surface and the imaging plane satisfying the condition is specifically as follows:

[0072] See also Figure 4 and Figure 5 (In the simplified figure, the laser ignores the volume and is regarded as a line segment) and establishes an equivalent model in the coordinate system, such as Figure 6As shown, the coordinates of point A are (0, d), the coordinates of point C are (e, 0), and the coordinates of point D are (e, h). When the volume of the laser is ignored, θ2 is the emission angle of the laser, and the path of the laser emitted by the laser is the line segment AE. If the focal length is to be coupled with the laser focal plane, the optical focus of the camera should also be located at point E. The focal length of the camera should be the distance from the optical center of the lens to the focus, so the following equation is satisfied:

[0073]

[0074] In the large triangle ABE:

[0075]

[0076] After finishing, we can get:

[0077]

[0078] Right now:

[0079]

[0080] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0081] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An infrared fluorescence camera focusing system with automatic coupling of excitation light and focal plane, characterized in that: It includes a dual-path excitation light source, a reboundable laser fixing frame, a focusing ring and an infrared fluorescence camera; the dual-path excitation light source is arranged on both sides of the infrared fluorescence camera to excite a specific contrast agent to generate a fluorescence signal; the reboundable laser fixing frame is close to the focusing ring and is used to fix the dual-path excitation light source, and the angle of the dual-path excitation light source is changed as the focusing ring rotates; the focusing ring is arranged in front of the infrared fluorescence camera to adjust the focal plane of the infrared fluorescence camera, and control the angle of the dual-path excitation light source to achieve coupling between the excitation light irradiation surface and the imaging plane.

2. The infrared fluorescence camera focusing system with automatic coupling of excitation light and focal plane according to claim 1, characterized in that: The focus ring is elliptical, and the range of the rotation angle θ is 0-90 degrees.

3. The infrared fluorescence camera focusing system with automatic coupling of excitation light and focal plane according to claim 2, characterized in that: The focal length of the infrared fluorescence camera changes as the focus ring rotates: f(θ)=f max +(f max -f min )·g(θ) Where θ is the rotation angle of the focus ring, f is the current focal length, and f max and f min are the maximum focal length and minimum focal length of the infrared fluorescence camera respectively, and g(θ) represents a nonlinear function that reflects the change of focal length with the rotation angle of the focus ring.

4. The infrared fluorescence camera focusing system with automatic coupling of excitation light and focal plane according to claim 3, characterized in that: The angle θ2 between the dual-path excitation light source and the fixed fulcrum changes with the rotation of the focusing ring: Where d is half of the distance between the two laser fixed points in the dual-path excitation light source, h is half of the length L of the tangent line at the center of the focusing ring ellipse, and e is the length of the infrared fluorescence camera.

5. The infrared fluorescence camera focusing system with automatic coupling of excitation light and focal plane according to claim 4, characterized in that: The length L of the tangent line at the center of the focusing ring ellipse changes as the focusing ring rotates: Wherein, a is half of the length of the major axis of the focusing ring ellipse, and b is half of the length of the minor axis of the focusing ring ellipse; when θ=0, L=2a; when θ=90, L=2b.

6. The infrared fluorescence camera focusing system with automatic coupling of excitation light and focal plane according to claim 5, characterized in that: If the excitation light irradiation surface is coupled to the imaging plane, then: The major axis length 2a and the minor axis length 2b of the focusing ring ellipse are obtained by calculation. Under this condition, the excitation light irradiation surface and the imaging plane are automatically coupled, and the imaging position is changed by adjusting the rotation angle θ of the focusing ring ellipse.

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