Animal body surface standardized grid coordinate system construction method based on anatomical markers
By constructing a standardized grid coordinate system for animal body surfaces based on anatomical marks, the problems of low acupoint sensitization positioning accuracy and lack of standardization are solved, and high-precision and low-cost acupoint sensitization area positioning and multimodal data analysis are realized.
Patent Information
- Application Number
- CN202510304541.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-14
- Publication Date
- 2025-04-25
AI Technical Summary
In the research on acupoint sensitization, the low accuracy of acupoint sensitization positioning and lack of standardization, which makes it difficult to achieve accurate expressions across experiments and models. The multimodal sensitization data lacks a unified spatial reference framework, which affects parameter correlation analysis and data comparison analysis.
A standardized grid coordinate system construction method based on animal body surfaces is adopted. By determining the coordinate axis, establishing coordinate grid grids and filling grid numbers, a systematic grid area is constructed to provide an accurate spatial reference framework.
It realizes standardized and quantifiable precise positioning, improves the positioning accuracy of acupoint sensitized areas, provides a unified spatial reference framework for multimodal data, supports cross-experimental data comparative analysis, and is significantly cost-saving and can replace high-cost image positioning equipment.
Smart Images
Figure CN119867958A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of animal body surface coordinate determination, and in particular to a method for constructing an animal body surface standardized grid coordinate system based on anatomical landmarks. Background Art
[0002] Acupoint sensitization is the core scientific proposition of the classic theory of traditional Chinese medicine that "what is manifested inside must be manifested outside". Its essence lies in revealing the dynamic relationship between the biological information transmission between the body surface and the internal organs under disease conditions. Modern research shows that acupoint sensitization has the characteristics of dynamics and diversity. Therefore, accurate positioning of sensitized areas is not only the core prerequisite for guiding clinical individualized intervention (such as acupuncture point selection), but also the research basis for exploring the manifestations and biological mechanisms of acupoint sensitization.
[0003] In clinical practice, acupoint sensitization positioning mainly relies on the operator's manual operation and experience judgment, such as exploring sensitive points on the body surface by cutting, tracing, pressing, etc. In addition, traditional acupoint positioning methods can also be used to locate sensitized sites, such as body surface landmarks (including fixed landmarks and movable landmarks), bone measurement method, and finger measurement method. Although these methods are of great value in TCM practice, they also have problems such as strong subjectivity and poor repeatability.
[0004] In animal experiments, due to differences in individual animals and body positions, traditional positioning methods are difficult to achieve accurate descriptions across experiments and models, resulting in the inability to accurately and standardizedly locate surface sensitization points. In addition, multimodal sensitization data such as Evans Blue (EB), pain threshold, and microcirculation (laser speckle) lack a unified spatial reference framework, resulting in the inability to perform correlation analysis between parameters, which in turn makes cross-experimental data difficult to compare and analyze due to inconsistent positioning benchmarks. Therefore, the field of experimental animal surface positioning urgently needs a low-cost, high-precision, and multimodal data-compatible standardized technical solution to break through the technical barriers of upgrading acupoint sensitization research from empirical description to spatial quantification. Summary of the invention
[0005] The purpose of the present invention is to solve the problems of low acupoint sensitization positioning accuracy and lack of standardization in acupoint sensitization research. Therefore, a method for constructing a standardized grid coordinate system on the animal surface based on anatomical landmarks is proposed to solve the above problems.
[0006] The following technical solutions are specifically adopted:
[0007] The construction methods include the following:
[0008] S1 Determine the coordinate axis: Take rats as experimental subjects, and use the rat back as the coordinate object. Determine the top of the spinous process of the first thoracic vertebra of the rat as the coordinate origin, the posterior midline of the body as the longitudinal axis, and the longitudinal axis mark extends caudally along the spine. The vertical line of the coronal plane is the horizontal axis, thereby determining the central longitudinal and horizontal axes of the grid coordinates. The longitudinal and horizontal axes are distributed in a T shape.
[0009] S2 establishes a coordinate grid: extends to the left and right sides with the vertical axis as the center, and extends downward with the horizontal axis as the starting point, forming a rectangular grid film with the length of the vertical axis as the long side and the length of the horizontal axis as the wide side, which is used to cover the body surface of the rat;
[0010] S3 fills in grid numbers: The horizontal axis is marked symmetrically on both sides. The horizontal axis origin is marked from 1 to 6 to the right, and the horizontal axis origin is marked from 11 to 16 to the left. The vertical axis is marked from the origin downward with letters A to V. The rat's back is divided into systematic grid areas through coordinate combination, where positions 6 and 16 on the horizontal axis correspond to the lowest points of the bilateral costal arches, respectively.
[0011] Preferably, in the operation of determining the coordinate axis in step S1, the letter identifiers A to V on the vertical axis are located in the vertical line area, and the digital identifiers distributed on the horizontal axis are located in the grid frame.
[0012] Preferably, in the operation of determining the coordinate axis in step S1, the rectangular film uses a polymer film as a material, and is stretched and formed through cold stretching and hot stretching processes. During the stretching process, the film is first cold stretched in a cold stretching zone, and then enters a hot stretching zone for hot stretching, and finally undergoes a high-temperature forming treatment to reduce internal stress and obtain a stable size.
[0013] Preferably, in the operation of determining the coordinate axis in step S1, when the object is established with the rat abdomen as the coordinate, the rat's sternal jugular notch is used as the origin, the upper edge of the pubic symphysis is used as the terminal mark of the longitudinal axis, the lowest point of the bilateral costal arches is used as the auxiliary calibration point of the horizontal axis, and the longitudinal axis mark extends from the ventral side to the caudal side, thereby systematically segmenting the rat's abdominal area.
[0014] Preferably, in the operation of determining the coordinate axis in step S1, when the rat hind limb is used as the coordinate to establish the object, the rat hind limb is maintained in an anatomical extended position as the basis, and the line from the highest point of the greater trochanter of the femur to the midpoint of the patella (or the lowest point of the popliteal fossa) to the midpoint of the line connecting the inner and outer malleolus is used as the dividing line of the coronal reference plane. The hind limb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the greater trochanter of the rat femur and the midpoint of the patella (or the lowest point of the popliteal fossa) are divided into three equal parts, and the thigh is divided into the proximal 1 / 3 area (area A), the middle 1 / 3 area (area B) and the distal 1 / 3 area (area C); the midpoint of the rat patella (or the lowest point of the popliteal fossa) and the midpoint of the line connecting the inner and outer malleolus are divided into three equal parts, and the calf is divided into the proximal 1 / 3 area (area D), the middle 1 / 3 area (area E) and the distal 1 / 3 area (area F), so as to construct a standardized positioning grid coordinate system for the hind limb.
[0015] Preferably, in the operation of determining the coordinate axis in step S1, when the rat forelimb is used as the coordinate to establish the object, the rat forelimb is maintained in the anatomical extended position as the basis, and the line from the highest point of the humeral head to the olecranon to the most distal end of the ulna is used as the dividing line of the coronal reference plane. With this dividing line as the boundary, the forelimb is divided into the ventral side and the dorsal side. The highest point of the rat humeral head and the olecranon are divided into three equal parts, and the proximal end of the forelimb is divided into the proximal 1 / 3 area (A zone), the middle 1 / 3 area (B zone) and the distal 1 / 3 area (C zone); the rat olecranon to the most distal end of the ulna is divided into three equal parts, and the distal end of the forelimb is divided into the proximal 1 / 3 area (D zone), the middle 1 / 3 area (E zone) and the distal 1 / 3 area (F zone), so as to construct a standardized positioning grid coordinate system for the forelimb.
[0016] Compared with the prior art, the present invention has the following beneficial effects:
[0017] 1. The present invention sets a grid-shaped coordinate system, selects different standard lines for comparison according to different positions on the rat, can systematically separate the corresponding areas, can achieve standardized and quantifiable precise positioning, can more accurately locate and analyze the position characteristics of the surface exudation points, selects stable anatomical landmarks on the rat body surface as the coordinate system reference, constructs a standardized grid coordinate system at different positions on the rat body surface, divides the rat back into systematic grid areas through coordinate combination, and then achieves quantifiable positioning of the area, provides an accurate spatial reference framework for acupoint sensitization research, has significant cost savings, and can replace high-cost image positioning equipment. In addition, since the present invention adopts a stretchable polymer material that can be fixed at high temperature, when the target rat is small, it can be directly trimmed. When the target rat is large in size, the device can be stretched and enlarged in proportion by high-temperature stretching and fixing, so that it can adapt to experimental rats of different sizes.
[0018] 2. The present invention can adapt to different usage requirements by dividing the coordinate system into six different types. The criss-cross line grid without a film can fully fit the body surface of the rat, which is convenient for locating and marking the uneven positions on the rat's body surface, and each grid is divided into four equal parts: upper right, lower right, upper left and lower left. Four holes are set in circular holes in the four areas for instruments to be inserted into the holes for fixed-point experimental operations. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 It is a schematic flow chart of a method for constructing a standardized grid coordinate system on the surface of an animal based on anatomical landmarks in the present invention;
[0020] Figure 2 It is a grid type 1 of a method for constructing a standardized grid coordinate system on an animal body surface based on anatomical landmarks in the present invention;
[0021] Figure 3 It is the grid type 2 of the method for constructing a standardized grid coordinate system of an animal body surface based on anatomical landmarks in the present invention;
[0022] Figure 4 It is the grid type 3 of the method for constructing a standardized grid coordinate system of an animal body surface based on anatomical landmarks in the present invention;
[0023] Figure 5 This is the grid type 4 of the method for constructing a standardized grid coordinate system on the surface of an animal based on anatomical landmarks in the present invention;
[0024] Figure 6 It is the grid type 5 of the method for constructing a standardized grid coordinate system of an animal body surface based on anatomical landmarks in the present invention;
[0025] Figure 7 This is grid type six of a method for constructing a standardized grid coordinate system on an animal body surface based on anatomical landmarks in the present invention. DETAILED DESCRIPTION
[0026] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.
[0027] Embodiment 1:
[0028] like Figure 1 and Figure 2 As shown, a method for constructing a standardized grid coordinate system on the surface of an animal based on anatomical landmarks includes the following steps:
[0029] S1 Determine the coordinate axis: Take rats as experimental subjects, and use the rat back as the coordinate object. Determine the top of the spinous process of the first thoracic vertebra of the rat as the coordinate origin, the posterior midline of the body as the longitudinal axis, and the longitudinal axis mark extends caudally along the spine. The vertical line of the coronal plane is the horizontal axis, thereby determining the central longitudinal and horizontal axes of the grid coordinates. The longitudinal and horizontal axes are distributed in a T shape.
[0030] S2 establishes a coordinate grid: extends to the left and right sides with the vertical axis as the center, and extends downward with the horizontal axis as the starting point, forming a rectangular grid film with the length of the vertical axis as the long side and the length of the horizontal axis as the wide side, which is used to cover the body surface of the rat;
[0031] S3 fills in grid numbers: The horizontal axis is marked symmetrically on both sides. From the origin of the horizontal axis, it is marked as 1 to 6 to the right, and from the origin of the horizontal axis to the left, it is marked as -1 to -6. The vertical axis is marked with letters 1 to 22 from the origin downward. The rat's back is divided into systematic grid areas through coordinate combination, where the 6th and -6th positions on the horizontal axis correspond to the lowest points of the bilateral costal arches, respectively.
[0032] Specifically, in the operation of determining the coordinate axis in step S1, the letter symbols A to V on the vertical axis are located in the vertical line area, and the digital symbols distributed on the horizontal axis are located in the grid frame.
[0033] Specifically, in the operation of determining the coordinate axis in step S1, the rectangular film uses a polymer film as a material, and is stretched and formed through cold stretching and hot stretching processes. During the stretching process, the film is first cold stretched in a cold stretching zone, and then enters a hot stretching zone for hot stretching, and finally undergoes a high-temperature forming treatment to reduce internal stress and obtain a stable size.
[0034] Specifically, in the operation of determining the coordinate axis in step S1, when the object is established with the rat abdomen as the coordinate, the rat's sternal jugular notch is used as the origin, the upper edge of the pubic symphysis is used as the terminal mark of the longitudinal axis, the lowest point of the bilateral costal arches is used as the auxiliary calibration point of the horizontal axis, and the longitudinal axis mark extends from the ventral side to the caudal side, thereby systematically segmenting the rat's abdominal area.
[0035] Specifically, in the operation of determining the coordinate axis in step S1, when the rat hind limb is used as the coordinate establishment object, the rat hind limb is maintained in the anatomical extension position as the basis, and the line from the highest point of the greater trochanter of the femur to the midpoint of the patella (or the lowest point of the popliteal fossa) to the midpoint of the line connecting the inner and outer malleolus is used as the dividing line of the coronal reference plane. The hind limb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the greater trochanter of the rat femur and the midpoint of the patella (or the lowest point of the popliteal fossa) are divided into three equal parts, and the thigh is divided into the proximal 1 / 3 area (area A), the middle 1 / 3 area (area B) and the distal 1 / 3 area (area C); the midpoint of the patella (or the lowest point of the popliteal fossa) of the rat and the midpoint of the line connecting the inner and outer malleolus are divided into three equal parts, and the calf is divided into the proximal 1 / 3 area (area D), the middle 1 / 3 area (area E) and the distal 1 / 3 area (area F), so as to construct a standardized positioning grid coordinate system for the hind limb.
[0036] Specifically, in the operation of determining the coordinate axis in step S1, when the rat forelimb is used as the coordinate to establish the object, the rat forelimb is maintained in the anatomical extended position as the basis, and the line from the highest point of the humeral head to the olecranon to the most distal end of the ulna is used as the dividing line of the coronal reference plane. The forelimb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the rat humeral head and the olecranon are divided into three equal parts, and the proximal end of the forelimb is divided into the proximal 1 / 3 area (Area A), the middle 1 / 3 area (Area B) and the distal 1 / 3 area (Area C); the olecranon to the most distal end of the ulna is divided into three equal parts, and the distal end of the forelimb is divided into the proximal 1 / 3 area (Area D), the middle 1 / 3 area (Area E) and the distal 1 / 3 area (Area F), so as to construct a standardized positioning grid coordinate system for the forelimb.
[0037] As can be seen from the above, this method sets a grid-like coordinate system, selects different standard lines for comparison according to different positions on the rat, can systematically separate the corresponding areas, can achieve standardized and quantifiable precise positioning, can more accurately locate and analyze the position characteristics of the surface exudation points, selects stable anatomical landmarks on the rat's body surface as the coordinate system reference, constructs a standardized grid coordinate system at different positions on the rat's body surface, divides the rat's back into systematic grid areas through coordinate combination, and then achieves quantifiable positioning of the area, provides an accurate spatial reference framework for acupoint sensitization research, has significant cost savings, and can replace high-cost image positioning equipment. In addition, since the present invention uses a stretchable polymer material that can be fixed at high temperature, when the target rat is small, it can be directly trimmed. When the target rat is large in size, the device can be stretched and enlarged in proportion by high-temperature stretching and fixing, so that it can adapt to experimental rats of different sizes.
[0038] Embodiment 2:
[0039] like Figure 3As shown, S1 determines the coordinate axis: rats are used as experimental objects, and the rat back is used as the coordinate object. The top of the spinous process of the first thoracic vertebra of the rat is determined as the coordinate origin, the posterior midline of the body is used as the longitudinal axis, and the longitudinal axis mark extends caudally along the spine. The vertical line of the coronal plane is used as the horizontal axis, thereby determining the central longitudinal and horizontal axes of the grid coordinates. The longitudinal and horizontal axes are distributed in a T shape.
[0040] S2 establishes a coordinate grid: extending to the left and right sides with the vertical axis as the center, and extending downward with the horizontal axis as the starting point, to form a rectangular grid line with the length of the vertical axis as the long side and the length of the horizontal axis as the wide side, which is used to cover the body surface of the rat;
[0041] S3 fill in grid numbers: The horizontal axis labels are symmetrically distributed on both sides, and the vertical axis is labeled with letters 1 to 22 from the origin downward. The rat's back is divided into systematic grid areas through coordinate combinations.
[0042] Specifically, the horizontal axis is marked symmetrically on both sides. The horizontal axis is marked from 1 to 6 from the origin to the right, and from -1 to -6 from the origin to the left. The vertical axis is marked with letters from A to V from the origin downward. The rat's back is divided into systematic grid areas through coordinate combination, where positions 6 and -6 on the horizontal axis correspond to the lowest points of the bilateral costal arches, respectively.
[0043] Specifically, in the operation of determining the coordinate axis in step S1, when the rat hind limb is used as the coordinate establishment object, the rat hind limb is maintained in the anatomical extension position as the basis, and the line from the highest point of the greater trochanter of the femur to the midpoint of the patella (or the lowest point of the popliteal fossa) to the midpoint of the line connecting the inner and outer malleolus is used as the dividing line of the coronal reference plane. The hind limb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the greater trochanter of the rat femur and the midpoint of the patella (or the lowest point of the popliteal fossa) are divided into three equal parts, and the thigh is divided into the proximal 1 / 3 area (area A), the middle 1 / 3 area (area B) and the distal 1 / 3 area (area C); the midpoint of the patella (or the lowest point of the popliteal fossa) of the rat and the midpoint of the line connecting the inner and outer malleolus are divided into three equal parts, and the calf is divided into the proximal 1 / 3 area (area D), the middle 1 / 3 area (area E) and the distal 1 / 3 area (area F), so as to construct a standardized positioning grid coordinate system for the hind limb.
[0044] Specifically, in the operation of determining the coordinate axis in step S1, when the rat forelimb is used as the coordinate to establish the object, the rat forelimb is maintained in the anatomical extended position as the basis, and the line from the highest point of the humeral head to the olecranon to the most distal end of the ulna is used as the dividing line of the coronal reference plane. The forelimb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the rat humeral head and the olecranon are divided into three equal parts, and the proximal end of the forelimb is divided into the proximal 1 / 3 area (Area A), the middle 1 / 3 area (Area B) and the distal 1 / 3 area (Area C); the olecranon to the most distal end of the ulna is divided into three equal parts, and the distal end of the forelimb is divided into the proximal 1 / 3 area (Area D), the middle 1 / 3 area (Area E) and the distal 1 / 3 area (Area F), so as to construct a standardized positioning grid coordinate system for the forelimb.
[0045] As can be seen from the above, the present invention sets a grid-shaped coordinate system, selects different standard lines for comparison according to different positions on the rat, can systematically separate the corresponding areas, can achieve standardized and quantifiable precise positioning, can more accurately locate and analyze the position characteristics of the surface exudation points, selects stable anatomical landmarks on the rat surface as the coordinate system reference, constructs a standardized grid coordinate system at different positions on the rat surface, divides the rat back into systematic grid areas through coordinate combination, and then achieves quantifiable positioning of the area, provides an accurate spatial reference framework for acupoint sensitization research, has significant cost savings, and can replace high-cost image positioning equipment, and because the present invention uses a stretchable polymer material that can be fixed at high temperature, when the target rat is small, it can be directly trimmed, and when the target rat is large, the device can be stretched and enlarged in proportion by high-temperature stretching and fixing, so that it can adapt to experimental rats of different sizes, and the criss-crossing line grid can be fully fitted with the rat's body surface, which is convenient for positioning and marking the uneven position of the rat's body surface.
[0046] Embodiment three:
[0047] like Figure 4 As shown, S1 determines the coordinate axis: rats are used as experimental objects, and the rat back is used as the coordinate object. The top of the spinous process of the first thoracic vertebra of the rat is determined as the coordinate origin, the posterior midline of the body is used as the longitudinal axis, and the longitudinal axis mark extends caudally along the spine. The vertical line of the coronal plane is used as the horizontal axis, thereby determining the central longitudinal and horizontal axes of the grid coordinates. The longitudinal and horizontal axes are distributed in a T shape.
[0048] S2 establishes a coordinate grid: extending to the left and right sides with the vertical axis as the center, and extending downward with the horizontal axis as the starting point, to form a rectangular grid film with the length of the vertical axis as the long side and the length of the horizontal axis as the wide side, which is used to cover the body surface of the rat, and each grid is divided into four equal parts: upper right, lower right, upper left and lower left, and circular holes are opened in the four areas;
[0049] S3 fills in grid numbers: The horizontal axis is marked symmetrically on both sides. From the origin of the horizontal axis, it is marked as A to E to the right, and from the origin of the horizontal axis to the left, it is marked as a to e. The vertical axis is marked with letters 1 to 22 from the origin downward. The rat's back is divided into systematic grid areas through coordinate combination, where the E and e positions on the horizontal axis correspond to the lowest points of the bilateral costal arches, respectively.
[0050] Specifically, in the operation of determining the coordinate axis in step S1, the letter symbols A to V on the vertical axis are located in the vertical line area, and the digital symbols distributed on the horizontal axis are located in the grid frame.
[0051] Specifically, in the operation of determining the coordinate axis in step S1, the rectangular film uses a polymer film as a material, and is stretched and formed through cold stretching and hot stretching processes. During the stretching process, the film is first cold stretched in a cold stretching zone, and then enters a hot stretching zone for hot stretching, and finally undergoes a high-temperature forming treatment to reduce internal stress and obtain a stable size.
[0052] Specifically, in the operation of determining the coordinate axis in step S1, when the object is established with the rat abdomen as the coordinate, the rat's sternal jugular notch is used as the origin, the upper edge of the pubic symphysis is used as the terminal mark of the longitudinal axis, the lowest point of the bilateral costal arches is used as the auxiliary calibration point of the horizontal axis, and the longitudinal axis mark extends from the ventral side to the caudal side, thereby systematically segmenting the rat's abdominal area.
[0053] Specifically, in the operation of determining the coordinate axis in step S1, when the rat hind limb is used as the coordinate establishment object, the rat hind limb is maintained in the anatomical extension position as the basis, and the line from the highest point of the greater trochanter of the femur to the midpoint of the patella (or the lowest point of the popliteal fossa) to the midpoint of the line connecting the inner and outer malleolus is used as the dividing line of the coronal reference plane. The hind limb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the greater trochanter of the rat femur and the midpoint of the patella (or the lowest point of the popliteal fossa) are divided into three equal parts, and the thigh is divided into the proximal 1 / 3 area (area A), the middle 1 / 3 area (area B) and the distal 1 / 3 area (area C); the midpoint of the patella (or the lowest point of the popliteal fossa) of the rat and the midpoint of the line connecting the inner and outer malleolus are divided into three equal parts, and the calf is divided into the proximal 1 / 3 area (area D), the middle 1 / 3 area (area E) and the distal 1 / 3 area (area F), so as to construct a standardized positioning grid coordinate system for the hind limb.
[0054] Specifically, in the operation of determining the coordinate axis in step S1, when the rat forelimb is used as the coordinate to establish the object, the rat forelimb is maintained in the anatomical extended position as the basis, and the line from the highest point of the humeral head to the olecranon to the most distal end of the ulna is used as the dividing line of the coronal reference plane. The forelimb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the rat humeral head and the olecranon are divided into three equal parts, and the proximal end of the forelimb is divided into the proximal 1 / 3 area (Area A), the middle 1 / 3 area (Area B) and the distal 1 / 3 area (Area C); the olecranon to the most distal end of the ulna is divided into three equal parts, and the distal end of the forelimb is divided into the proximal 1 / 3 area (Area D), the middle 1 / 3 area (Area E) and the distal 1 / 3 area (Area F), so as to construct a standardized positioning grid coordinate system for the forelimb.
[0055] As can be seen from the above, the present invention sets a grid-shaped coordinate system, selects different standard lines for comparison according to different positions on the rat, can systematically separate the corresponding areas, can achieve standardized and quantifiable precise positioning, can more accurately locate and analyze the position characteristics of the surface exudation points, selects stable anatomical landmarks on the rat's body surface as the coordinate system reference, constructs a standardized grid coordinate system at different positions on the rat's body surface, divides the rat's back into systematic grid areas through coordinate combination, and then achieves quantifiable positioning of the area, provides an accurate spatial reference framework for acupoint sensitization research, and has significant cost savings. It can replace high-cost image positioning equipment, and because the present invention uses a stretchable polymer material that can be fixed at high temperature, when the target rat is small, it can be directly trimmed. When the target rat is large in size, the device can be stretched and enlarged in proportion by high-temperature stretching and fixing, so that it can adapt to experimental rats of different sizes, and four holes are set in the grid for the instrument to be inserted into the hole for fixed-point experimental operation.
[0056] Embodiment 4:
[0057] like Figure 5 As shown, a method for constructing a standardized grid coordinate system on the surface of an animal based on anatomical landmarks includes the following steps:
[0058] S1 Determine the coordinate axis: Take rats as experimental subjects, and use the rat back as the coordinate object. Determine the top of the spinous process of the first thoracic vertebra of the rat as the coordinate origin, the posterior midline of the body as the longitudinal axis, and the longitudinal axis mark extends caudally along the spine. The vertical line of the coronal plane is the horizontal axis, thereby determining the central longitudinal and horizontal axes of the grid coordinates. The longitudinal and horizontal axes are distributed in a T shape.
[0059] S2 establishes a coordinate grid: extends to the left and right sides with the vertical axis as the center, and extends downward with the horizontal axis as the starting point, forming a rectangular grid film with the length of the vertical axis as the long side and the length of the horizontal axis as the wide side, which is used to cover the body surface of the rat;
[0060] S3 fills in grid numbers: The horizontal axis is marked symmetrically on both sides. From the origin of the horizontal axis, it is marked as 1 to 6 to the right, and from the origin of the horizontal axis to the left, it is marked as -1 to -6. The vertical axis is marked with letters 1 to 22 from the origin downward. The rat's back is divided into systematic grid areas through coordinate combination, where the 6th and -6th positions on the horizontal axis correspond to the lowest points of the bilateral costal arches, respectively.
[0061] Specifically, in the operation of determining the coordinate axis in step S1, the rectangular film uses a polymer film as a material, and is stretched and formed through cold stretching and hot stretching processes. During the stretching process, the film is first cold stretched in a cold stretching zone, and then enters a hot stretching zone for hot stretching, and finally undergoes a high-temperature forming treatment to reduce internal stress and obtain a stable size.
[0062] Specifically, in the operation of determining the coordinate axis in step S1, when the object is established with the rat abdomen as the coordinate, the rat's sternal jugular notch is used as the origin, the upper edge of the pubic symphysis is used as the terminal mark of the longitudinal axis, the lowest point of the bilateral costal arches is used as the auxiliary calibration point of the horizontal axis, and the longitudinal axis mark extends from the ventral side to the caudal side, thereby systematically segmenting the rat's abdominal area.
[0063] Specifically, in the operation of determining the coordinate axis in step S1, when the rat hind limb is used as the coordinate establishment object, the rat hind limb is maintained in the anatomical extension position as the basis, and the line from the highest point of the greater trochanter of the femur to the midpoint of the patella (or the lowest point of the popliteal fossa) to the midpoint of the line connecting the inner and outer malleolus is used as the dividing line of the coronal reference plane. The hind limb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the greater trochanter of the rat femur and the midpoint of the patella (or the lowest point of the popliteal fossa) are divided into three equal parts, and the thigh is divided into the proximal 1 / 3 area (area A), the middle 1 / 3 area (area B) and the distal 1 / 3 area (area C); the midpoint of the patella (or the lowest point of the popliteal fossa) of the rat and the midpoint of the line connecting the inner and outer malleolus are divided into three equal parts, and the calf is divided into the proximal 1 / 3 area (area D), the middle 1 / 3 area (area E) and the distal 1 / 3 area (area F), so as to construct a standardized positioning grid coordinate system for the hind limb.
[0064] Specifically, in the operation of determining the coordinate axis in step S1, when the rat forelimb is used as the coordinate to establish the object, the rat forelimb is maintained in the anatomical extended position as the basis, and the line from the highest point of the humeral head to the olecranon to the most distal end of the ulna is used as the dividing line of the coronal reference plane. The forelimb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the rat humeral head and the olecranon are divided into three equal parts, and the proximal end of the forelimb is divided into the proximal 1 / 3 area (Area A), the middle 1 / 3 area (Area B) and the distal 1 / 3 area (Area C); the olecranon to the most distal end of the ulna is divided into three equal parts, and the distal end of the forelimb is divided into the proximal 1 / 3 area (Area D), the middle 1 / 3 area (Area E) and the distal 1 / 3 area (Area F), so as to construct a standardized positioning grid coordinate system for the forelimb.
[0065] As can be seen from the above, this method sets a grid-like coordinate system, selects different standard lines for comparison according to different positions on the rat, can systematically separate the corresponding areas, can achieve standardized and quantifiable precise positioning, can more accurately locate and analyze the position characteristics of the surface exudation points, selects stable anatomical landmarks on the rat's body surface as the coordinate system reference, constructs a standardized grid coordinate system at different positions on the rat's body surface, divides the rat's back into systematic grid areas through coordinate combination, and then achieves quantifiable positioning of the area, provides an accurate spatial reference framework for acupoint sensitization research, has significant cost savings, and can replace high-cost image positioning equipment. In addition, since the present invention uses a stretchable polymer material that can be fixed at high temperature, when the target rat is small, it can be directly trimmed. When the target rat is large in size, the device can be stretched and enlarged in proportion by high-temperature stretching and fixing, so that it can adapt to experimental rats of different sizes.
[0066] Embodiment five:
[0067] like Figure 6 As shown, a method for constructing a standardized grid coordinate system on the surface of an animal based on anatomical landmarks includes the following steps:
[0068] S1 Determine the coordinate axis: Take rats as experimental subjects, and use the rat back as the coordinate object. Determine the top of the spinous process of the first thoracic vertebra of the rat as the coordinate origin, the posterior midline of the body as the longitudinal axis, and the longitudinal axis mark extends caudally along the spine. The vertical line of the coronal plane is the horizontal axis, thereby determining the central longitudinal and horizontal axes of the grid coordinates. The longitudinal and horizontal axes are distributed in a T shape.
[0069] S2 establishes a coordinate grid: extends to the left and right sides with the vertical axis as the center, and extends downward with the horizontal axis as the starting point, forming a rectangular grid film with the length of the vertical axis as the long side and the length of the horizontal axis as the wide side, which is used to cover the body surface of the rat;
[0070] S3 fills in grid numbers: The horizontal axis is marked symmetrically on both sides. The horizontal axis origin is marked as 1 to 6 to the right, and the horizontal axis origin is marked as -1 to -6 to the left. The vertical axis is marked with letters from A to V from the origin downward. The rat's back is divided into systematic grid areas through coordinate combination. The 6th and -6th positions on the horizontal axis correspond to the lowest points of the bilateral costal arches, respectively. The negative numbers in the grid can be changed to positive numbers according to the research habits.
[0071] Specifically, in the operation of determining the coordinate axis in step S1, the letter symbols A to Z on the vertical axis are located in the vertical line area, and the digital symbols distributed on the horizontal axis are located in the grid frame.
[0072] Specifically, in the operation of determining the coordinate axis in step S1, the rectangular film uses a polymer film as a material, and is stretched and formed through cold stretching and hot stretching processes. During the stretching process, the film is first cold stretched in a cold stretching zone, and then enters a hot stretching zone for hot stretching, and finally undergoes a high-temperature forming treatment to reduce internal stress and obtain a stable size.
[0073] Specifically, in the operation of determining the coordinate axis in step S1, when the object is established with the rat abdomen as the coordinate, the rat's sternal jugular notch is used as the origin, the upper edge of the pubic symphysis is used as the terminal mark of the longitudinal axis, the lowest point of the bilateral costal arches is used as the auxiliary calibration point of the horizontal axis, and the longitudinal axis mark extends from the ventral side to the caudal side, thereby systematically segmenting the rat's abdominal area.
[0074] Specifically, in the operation of determining the coordinate axis in step S1, when the rat hind limb is used as the coordinate establishment object, the rat hind limb is maintained in the anatomical extension position as the basis, and the line from the highest point of the greater trochanter of the femur to the midpoint of the patella (or the lowest point of the popliteal fossa) to the midpoint of the line connecting the inner and outer malleolus is used as the dividing line of the coronal reference plane. The hind limb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the greater trochanter of the rat femur and the midpoint of the patella (or the lowest point of the popliteal fossa) are divided into three equal parts, and the thigh is divided into the proximal 1 / 3 area (area A), the middle 1 / 3 area (area B) and the distal 1 / 3 area (area C); the midpoint of the patella (or the lowest point of the popliteal fossa) of the rat and the midpoint of the line connecting the inner and outer malleolus are divided into three equal parts, and the calf is divided into the proximal 1 / 3 area (area D), the middle 1 / 3 area (area E) and the distal 1 / 3 area (area F), so as to construct a standardized positioning grid coordinate system for the hind limb.
[0075] Specifically, in the operation of determining the coordinate axis in step S1, when the rat forelimb is used as the coordinate to establish the object, the rat forelimb is maintained in the anatomical extended position as the basis, and the line from the highest point of the humeral head to the olecranon to the most distal end of the ulna is used as the dividing line of the coronal reference plane. The forelimb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the rat humeral head and the olecranon are divided into three equal parts, and the proximal end of the forelimb is divided into the proximal 1 / 3 area (area A), the middle 1 / 3 area (area B) and the distal 1 / 3 area (area C); the olecranon to the most distal end of the ulna is divided into three equal parts, and the distal end of the forelimb is divided into the proximal 1 / 3 area (area D), the middle 1 / 3 area (area E) and the distal 1 / 3 area (area F), so as to construct a standardized positioning grid coordinate system for the forelimb. The form of the table also includes a laser projection method. When used, a laser spotlight can be used to form a virtual grid pattern to illuminate the rat specimen for reference.
[0076] As can be seen from the above, this method sets a grid-like coordinate system, selects different standard lines for comparison according to different positions on the rat, can systematically separate the corresponding areas, can achieve standardized and quantifiable precise positioning, can more accurately locate and analyze the position characteristics of the surface exudation points, selects stable anatomical landmarks on the rat's body surface as the coordinate system reference, constructs a standardized grid coordinate system at different positions on the rat's body surface, divides the rat's back into systematic grid areas through coordinate combination, and then achieves quantifiable positioning of the area, provides an accurate spatial reference framework for acupoint sensitization research, has significant cost savings, and can replace high-cost image positioning equipment. In addition, since the present invention uses a stretchable polymer material that can be fixed at high temperature, when the target rat is small, it can be directly trimmed. When the target rat is large in size, the device can be stretched and enlarged in proportion by high-temperature stretching and fixing, so that it can adapt to experimental rats of different sizes.
[0077] Embodiment five:
[0078] like Figure 7 As shown, a method for constructing a standardized grid coordinate system on the surface of an animal based on anatomical landmarks includes the following steps:
[0079] S1 Determine the coordinate axis: Take rats as experimental subjects, and use the rat back as the coordinate object. Determine the top of the spinous process of the first thoracic vertebra of the rat as the coordinate origin, the posterior midline of the body as the longitudinal axis, and the longitudinal axis mark extends caudally along the spine. The vertical line of the coronal plane is the horizontal axis, thereby determining the central longitudinal and horizontal axes of the grid coordinates. The longitudinal and horizontal axes are distributed in a T shape.
[0080] S2 establishes a coordinate grid: extends to the left and right sides with the vertical axis as the center, and extends downward with the horizontal axis as the starting point, forming a rectangular grid film with the length of the vertical axis as the long side and the length of the horizontal axis as the wide side, which is used to cover the body surface of the rat;
[0081] S3 fills in grid numbers: The horizontal axis is marked symmetrically on both sides. From the origin of the horizontal axis, it is marked as 1 to 6 to the right, and from the origin of the horizontal axis, it is marked as 11 to 16 to the left. The vertical axis is marked with letters from A to V from the origin downward. The rat's back is divided into systematic grid areas through coordinate combination. The 6th and 16th positions on the horizontal axis correspond to the lowest points of the bilateral costal arches, respectively.
[0082] Specifically, in the operation of determining the coordinate axis in step S1, the letter symbols A to Z on the vertical axis are located in the vertical line area, and the digital symbols distributed on the horizontal axis are located in the grid frame.
[0083] Specifically, in the operation of determining the coordinate axis in step S1, the rectangular film uses a polymer film as a material, and is stretched and formed through cold stretching and hot stretching processes. During the stretching process, the film is first cold stretched in a cold stretching zone, and then enters a hot stretching zone for hot stretching, and finally undergoes a high-temperature forming treatment to reduce internal stress and obtain a stable size.
[0084] Specifically, in the operation of determining the coordinate axis in step S1, when the object is established with the rat abdomen as the coordinate, the rat's sternal jugular notch is used as the origin, the upper edge of the pubic symphysis is used as the terminal mark of the longitudinal axis, the lowest point of the bilateral costal arches is used as the auxiliary calibration point of the horizontal axis, and the longitudinal axis mark extends from the ventral side to the caudal side, thereby systematically segmenting the rat's abdominal area.
[0085] Specifically, in the operation of determining the coordinate axis in step S1, when the rat hind limb is used as the coordinate establishment object, the rat hind limb is maintained in the anatomical extension position as the basis, and the line from the highest point of the greater trochanter of the femur to the midpoint of the patella (or the lowest point of the popliteal fossa) to the midpoint of the line connecting the inner and outer malleolus is used as the dividing line of the coronal reference plane. The hind limb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the greater trochanter of the rat femur and the midpoint of the patella (or the lowest point of the popliteal fossa) are divided into three equal parts, and the thigh is divided into the proximal 1 / 3 area (area A), the middle 1 / 3 area (area B) and the distal 1 / 3 area (area C); the midpoint of the patella (or the lowest point of the popliteal fossa) of the rat and the midpoint of the line connecting the inner and outer malleolus are divided into three equal parts, and the calf is divided into the proximal 1 / 3 area (area D), the middle 1 / 3 area (area E) and the distal 1 / 3 area (area F), so as to construct a standardized positioning grid coordinate system for the hind limb.
[0086] Specifically, in the operation of determining the coordinate axis in step S1, when the rat forelimb is used as the coordinate to establish the object, the rat forelimb is maintained in the anatomical extended position as the basis, and the line from the highest point of the humeral head to the olecranon to the most distal end of the ulna is used as the dividing line of the coronal reference plane. The forelimb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the rat humeral head and the olecranon are divided into three equal parts, and the proximal end of the forelimb is divided into the proximal 1 / 3 area (area A), the middle 1 / 3 area (area B) and the distal 1 / 3 area (area C); the olecranon to the most distal end of the ulna is divided into three equal parts, and the distal end of the forelimb is divided into the proximal 1 / 3 area (area D), the middle 1 / 3 area (area E) and the distal 1 / 3 area (area F), so as to construct a standardized positioning grid coordinate system for the forelimb. The form of the table also includes a laser projection method. When used, a laser spotlight can be used to form a virtual grid pattern to illuminate the rat specimen for reference.
[0087] As can be seen from the above, this method sets a grid-like coordinate system, selects different standard lines for comparison according to different positions on the rat, can systematically separate the corresponding areas, can achieve standardized and quantifiable precise positioning, can more accurately locate and analyze the position characteristics of surface exudation points, selects stable anatomical landmarks on the rat's body surface as the coordinate system reference, constructs a standardized grid coordinate system at different positions on the rat's body surface, divides the rat's back into systematic grid areas through coordinate combination, and then achieves quantifiable positioning of the area, provides an accurate spatial reference framework for acupoint sensitization research, has significant cost savings, and can replace high-cost image positioning equipment. In addition, since the present invention uses a stretchable polymer material that can be fixed at high temperature, when the target rat is small, it can be directly trimmed. When the target rat is large in size, the device can be stretched and enlarged in proportion by high-temperature stretching and fixing, so that it can adapt to experimental rats of different sizes.
[0088] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. The replacement may be a replacement of a part of the structure, device, method step, or a complete technical solution. Any equivalent replacement or change according to the technical solution and the inventive concept of the present invention shall be covered within the protection scope of the present invention.
Claims
1. A method for constructing a standardized grid coordinate system on an animal body surface based on anatomical landmarks, characterized in that: The following steps are involved: S1 Determine the coordinate axis: Take rats as experimental subjects, and use the rat back as the coordinate object. Determine the top of the spinous process of the first thoracic vertebra of the rat as the coordinate origin, the posterior midline of the body as the longitudinal axis, and the longitudinal axis mark extends caudally along the spine. The vertical line of the coronal plane is the horizontal axis, thereby determining the central longitudinal and horizontal axes of the grid coordinates. The longitudinal and horizontal axes are distributed in a T shape. S2 establishes a coordinate grid: extends to the left and right sides with the vertical axis as the center, and extends downward with the horizontal axis as the starting point, forming a rectangular grid film with the length of the vertical axis as the long side and the length of the horizontal axis as the wide side, which is used to cover the body surface of the rat; S3 fills in grid numbers: The horizontal axis is marked symmetrically on both sides. The horizontal axis origin is marked from 1 to 6 to the right, and the horizontal axis origin is marked from 11 to 16 to the left. The vertical axis is marked from the origin downward with letters A to V. The rat's back is divided into systematic grid areas through coordinate combination, where positions 6 and 16 on the horizontal axis correspond to the lowest points of the bilateral costal arches, respectively.
2. A method for constructing a standardized grid coordinate system on an animal body surface based on anatomical landmarks as claimed in claim 1, characterized in that: In the operation of determining the coordinate axis in step S1, the letter symbols A to V on the vertical axis are located in the vertical line area, and the digital symbols distributed on the horizontal axis are located in the grid frame.
3. The method for constructing a standardized grid coordinate system on the surface of an animal based on anatomical landmarks according to claim 1, characterized in that: In the operation of determining the coordinate axis in step S1, the rectangular film uses a polymer film as a material, and is stretched and shaped through cold stretching and hot stretching processes. During the stretching process, the film is first cold stretched in a cold stretching zone, and then enters a hot stretching zone for hot stretching, and finally undergoes a high-temperature shaping treatment to reduce internal stress and obtain a stable size.
4. The method for constructing a standardized grid coordinate system on the surface of an animal based on anatomical landmarks according to claim 1, characterized in that: In the operation of determining the coordinate axis in step S1, when the object is established with the rat abdomen as the coordinate, the rat's sternal jugular notch is used as the origin, the upper edge of the pubic symphysis is used as the terminal mark of the longitudinal axis, the lowest point of the bilateral costal arches is used as the auxiliary calibration point of the horizontal axis, and the longitudinal axis mark extends from the ventral side to the caudal side, thereby systematically segmenting the rat's abdominal area.
5. The method for constructing a standardized grid coordinate system on the surface of an animal based on anatomical landmarks according to claim 1, characterized in that: In the operation of determining the coordinate axis in step S1, when the rat hind limb is used as the coordinate establishment object, the rat hind limb is maintained in the anatomical extension position as the basis, and the line connecting the highest point of the greater trochanter of the femur to the midpoint of the patella and the midpoint of the line connecting the medial and lateral malleolus is used as the dividing line of the coronal reference plane. The hind limb is divided into the ventral side and the dorsal side based on this dividing line. The area between the highest point of the greater trochanter of the rat femur and the midpoint of the patella is divided into three equal parts, and the thigh is divided into the proximal 1 / 3 area, the middle 1 / 3 area and the distal 1 / 3 area; the midpoint of the rat patella and the midpoint of the line connecting the medial and lateral malleolus are divided into three equal parts, and the calf is divided into the proximal 1 / 3 area, the middle 1 / 3 area and the distal 1 / 3 area, so as to construct a standardized positioning grid coordinate system for the hind limbs.
6. The method for constructing a standardized grid coordinate system on the surface of an animal based on anatomical landmarks according to claim 1, characterized in that: In the operation of determining the coordinate axis in step S1, when the rat forelimb is used as the coordinate to establish the object, the rat forelimb is maintained in the anatomical extended position as the basis, and the line from the highest point of the humeral head to the olecranon to the most distal end of the ulna is used as the dividing line of the coronal reference plane. The forelimb is divided into the ventral side and the dorsal side based on this dividing line. The highest point of the rat humeral head and the olecranon are divided into three equal parts, and the proximal end of the forelimb is divided into the proximal 1 / 3 area, the middle 1 / 3 area and the distal 1 / 3 area; the olecranon to the most distal end of the ulna is divided into three equal parts, and the distal end of the forelimb is divided into the proximal 1 / 3 area, the middle 1 / 3 area and the distal 1 / 3 area, so as to construct a standardized positioning grid coordinate system for the forelimb.