Device and method for detecting in-vitro and in-vivo components of colquhoumia root sheet

Abstract

The invention relates to a device and a method for detecting in-vitro and in-vivo components of a colquhoumia root sheet. The device comprises a vertical bracket, a fixing unit, a head limiting assembly, an upper limb limiting assembly, a lower limb limiting assembly and a medicine supply assembly, according to the invention, the following problems existing in the process of in-vivo and in-vitro component detection of the tripterygium hypoglaucum root slice in the prior art can be solved: a laboratory mouse cannot be limited and fixed, so that the laboratory mouse is easily frightened to randomly move and bounce, and great difficulty is caused to drenching and blood sampling; in the prior art, colquhoumia root tablet medicines with different concentrations cannot be infused into a plurality of laboratory mice at the same time, so that the plurality of laboratory mice need to be sequentially drenched, and the detection efficiency is influenced; the experimental mouse can be limited and fixed in all directions, so that the stability of the experimental mouse is ensured, and the experimental mouse is prevented from randomly bouncing to influence the drenching and blood sampling progress; and different concentrations of medicines can be infused into different laboratory mice, so that the detection of various different concentrations of medicine components can be completed at the same time.

Description

Technical Field

[0001] The present invention relates to the technical field of drug detection, and in particular to a device and method for detecting in vivo and in vitro components of a torch flower root tablet. Background Art

[0002] Torch flower root tablets are made from the roots of Kunming Crabapple. They have the effects of dispelling wind and dampness, relaxing muscles and tendons, clearing away heat and detoxifying, and have obvious inhibitory effects on pathological immune responses and anti-inflammatory and analgesic effects. In order to determine the specific content of each component in the torch flower root tablets, as well as to evaluate the safety of the torch flower root tablets and the biological activity of each component during the test, it is necessary to conduct corresponding in vivo and in vitro component tests on the torch flower root tablets. Animal models (such as experimental mice, etc.) are usually used for in vivo and in vitro testing to ensure its safety in clinical applications.

[0003] In the prior art, a large number of detection methods and corresponding devices for Torch Flower Root Tablets have been disclosed. For example, a Chinese patent with publication number CN115407005A discloses a method for detecting the in vivo and in vitro components of Torch Flower Root Tablets. When detecting Torch Flower Root Tablets, 15 g / kg of Torch Flower Root Tablets extract is administered orally to experimental mice; after 2 hours, blood is collected through the orbital venous plexus, placed in a centrifuge tube with sodium heparin and centrifuged at 4000 rpm for 20 minutes, the supernatant is frozen at -80°C to obtain serum; and an ultra-high pressure liquid chromatography time-of-flight mass spectrometer is used to detect and analyze the serum in positive ion mode and negative ion mode respectively.

[0004] However, in the process of using the above-mentioned prior art to test the root tablets of Torch Flower in vitro and in vivo using experimental mice, there are still the following shortcomings: 1. Although the above-mentioned prior art can infuse the laboratory mice with a specified concentration of torch flower root tablets and collect blood samples after a specified time, the laboratory mice cannot be fixed in position during this period. Therefore, the laboratory mice are easily frightened and move around at will, which causes great difficulties in infusing the medicine and collecting blood. It is also easy to make it difficult to accurately collect blood from the specified position, resulting in changes in the blood collection position and affecting the accuracy of blood collection, which in turn affects the test results.

[0005] 2. Since the above-mentioned prior art can only use one experimental mouse for torch flower root tablets testing at a time, it is impossible to infuse different concentrations of torch flower root tablets into multiple experimental mice at the same time, so it is necessary to infuse the drugs into multiple experimental mice in turn, which affects the detection efficiency.

[0006] 3. In order to ensure the accuracy of the test results, it is usually necessary to inject the torch flower root tablets into the experimental mice and place them in different temperature environments for a certain period of time before blood sampling. However, the above-mentioned existing technology cannot make corresponding adjustments to the environment in which the experimental mice are located, thereby failing to enhance the detection diversity and affecting the detection accuracy.

[0007] Therefore, based on the above-stated viewpoints, there is still room for improvement in the existing technology of detecting torch flower root pieces. Summary of the invention

[0008] In order to solve the above problems, the present invention provides a device for detecting the internal and external components of a torch flower root piece, comprising a vertical bracket, on which a plurality of fixing units are equidistantly arranged along the length direction, and the fixing units include a head limiting component, an upper limb limiting component and a lower limb limiting component distributed in sequence from top to bottom.

[0009] The head limiting assembly includes a pad installed on the side wall of the vertical bracket and close to the upper side, the upper limb limiting assembly is arranged on two positioning blocks on the side wall of the vertical bracket, the two positioning blocks are both located below the pad and symmetrically distributed along its width direction, and the lower limb limiting assembly includes an installation block located below the two positioning blocks.

[0010] As a preferred technical solution of the present invention, the fixed unit also includes a drug supply component for infusing the experimental mice with the torch flower root tablets. The drug supply component includes a plurality of drug storage barrels installed on the outer wall of the vertical bracket away from the fixed unit. The plurality of drug storage barrels correspond to the positions of the plurality of fixed units. A delivery pump is installed on the drug storage barrel. The output end of the delivery pump is connected to a drug delivery tube. The end of the drug delivery tube away from the delivery pump passes through the vertical bracket and extends to the pad.

[0011] As a preferred technical solution of the present invention, the head limit assembly also includes an arc-shaped groove opened on the side of the pad away from the vertical bracket, a rubber pad is installed on the inner wall of the arc-shaped groove, and a plurality of anti-slip rubber strips are installed at equal distances along the width direction on the side of the rubber pad away from the pad; A plurality of mounting holes are provided on both sides of the inner wall of the arc-shaped groove close to the length direction of the pad, elastic telescopic rods are arranged in the mounting holes, and a protective ball is rotatably installed at one end of the elastic telescopic rod away from the pad.

[0012] As a preferred technical solution of the present invention, the head limit assembly also includes two ferrule groups installed on the side of the pad away from the vertical bracket, the two ferrule groups are symmetrically distributed along the length direction of the pad, and the ferrule groups each include two support plates symmetrically distributed along the rubber pad, and pull ropes are installed on the support plates, and a mouth sleeve is commonly provided between the two pull ropes of the same ferrule group.

[0013] As a preferred technical solution of the present invention, the ends of the two pull ropes on the same side of the two muzzles are jointly installed with a threaded pull rod after passing through the support plate, and two positioning frames are symmetrically arranged along the muzzle on the outer wall of the pad, and the positioning frames are equipped with fixing nuts sleeved on the outer wall of the threaded pull rod, and the fixing nuts and the threaded pull rod are matched with each other by a threaded connection.

[0014] As a preferred technical solution of the present invention, the upper limb limiting assembly includes a limiting slide groove provided on the side of the positioning block away from the vertical bracket, a displacement block is slidably docked inside the limiting slide groove, an L-shaped groove is provided on the displacement block, a through hole penetrating the positioning block is provided on the bottom wall of the limiting slide groove, and the vertical section of the L-shaped groove is connected to the through hole; A flexible belt connected end to end is slidably provided in the L-shaped groove. One side of the flexible belt passes through the horizontal section of the L-shaped groove to form a limit ring, and the other side of the flexible belt passes through the vertical section of the L-shaped groove and extends into the through hole. An adjusting branch chain is installed between the positioning block and the displacement block.

[0015] As a preferred technical solution of the present invention, the adjusting branch chain includes a connecting groove, and two connecting grooves are symmetrically opened on the inner wall of the limiting slide groove along the displacement block. Two linkage blocks that are slidably docked in the connecting grooves are installed on the outer wall of the displacement block. Threaded rods are rotatably arranged in the two connecting grooves, and the threaded rods pass through the linkage blocks by means of threaded connection.

[0016] As a preferred technical solution of the present invention, the lower limb limit assembly includes an actuator plate installed on the side of the vertical bracket close to the pad, the actuator plate is located below the two positioning blocks, a height adjustment branch chain is installed between the actuator plate and the vertical bracket, a mounting block and two supporting plates are arranged on the side of the actuator plate away from the vertical bracket, the two supporting plates are symmetrically distributed along the mounting block, a rotating shaft is rotatably arranged between the two supporting plates on the same actuator plate, the rotating shaft rotates through the mounting block, and two adjacent rotating shafts are connected by a linkage shaft; Two wire wheels are symmetrically mounted along the mounting block on the outer wall of the rotating shaft and are respectively located below the positioning blocks. A connecting rope is wound around the outer wall of the wire wheel. The end of the connecting rope away from the wire wheel passes upward through the through hole and is connected to the flexible belt. A tightening rope is also wound around the outer wall of the rotating shaft, and a tightening ring is provided on the side of the tightening rope away from the execution plate.

[0017] As a preferred technical solution of the present invention, the height adjustment branch chain includes a sliding groove, and the side of the vertical bracket close to the execution plate has a plurality of linkage groups corresponding to the position of the execution plate, each linkage group includes two sliding grooves symmetrically distributed along the length direction of the execution plate, and the side of the execution plate close to the vertical bracket is equipped with two auxiliary bars that are slidably docked in the sliding groove; A connecting shaft is rotatably arranged on the side of the vertical bracket away from the actuator plate through a fixed frame, and a plurality of rope pulleys corresponding to the position of the actuator plate are sleeved on the outer wall of the connecting shaft. Winding ropes are installed at the upper and lower ends of the auxiliary strip, and the ends of the two winding ropes away from the auxiliary strip pass through the vertical bracket and are wound around the rope pulleys together.

[0018] In addition, the present invention also provides a method for detecting the internal and external components of the torch flower root slice, comprising the following steps: S1: Placement of experimental mice: First, place the experimental mice at the fixed unit of the vertical stand; S2: Fixing the head of the experimental mouse: The head of the experimental mouse is fixed by the head limiting component; S3: Fixation of the limbs of the experimental mouse: The limbs of the experimental mouse are fixed by the upper limb limit assembly and the lower limb limit assembly; S4: Instilling medicine into experimental mice and collecting blood for testing: Infuse the experimental mice with a specified concentration of Torch Flower Root Tablets through their mouths. After a specified period of time, collect blood from the experimental mice's orbital venous plexus and conduct corresponding analysis and testing on the collected blood samples, thereby completing the in vivo and in vitro component testing of the Torch Flower Root Tablets.

[0019] In summary, the present application includes at least one of the following beneficial technical effects: 1. The present invention uses a head limiting component, an upper limb limiting component and a lower limb limiting component to limit and fix the experimental mouse in all directions, thereby ensuring the stability of the experimental mouse during the infusion of the Torch Flower Root Tablets and blood sampling, and preventing the experimental mouse from moving at will and affecting the progress of the infusion and blood sampling; and different concentrations of the Torch Flower Root Tablets can be infused into different experimental mice respectively, so that the detection of multiple drug components with different concentrations can be completed at the same time, thereby enhancing the detection effect and detection efficiency.

[0020] 2. The present invention places the head of the experimental mouse against the pad so that the head of the experimental mouse contacts with a plurality of anti-skid rubber strips. The anti-skid rubber strips can prevent the head of the experimental mouse from moving at will and affecting the infusion of drugs and the blood collection process. In addition, a plurality of elongated elastic telescopic rods are used to block the outside of the head of the experimental mouse to further limit the position and prevent the head of the experimental mouse from shaking at will, thereby enhancing the stability of the experimental mouse during the detection process.

[0021] 3. The present invention further limits and fixes the head of the experimental mouse by fastening two muzzles on the upper and lower jaws of the experimental mouse respectively, and can ensure that the mouth of the experimental mouse is open, so as to improve the infusion effect of the Torch Flower Root Tablets drug; in addition, the head swing of the experimental mouse can be controlled by offsetting and tightening the muzzle, so that the blood collection site of the experimental mouse is exposed and then limited and fixed, which not only facilitates the infusion of drugs, but also allows rapid blood collection, and can effectively improve the detection efficiency.

[0022] Fourth, the present invention inserts the two upper limbs of the experimental mouse into two limiting rings for limiting and fixing, thereby preventing the upper limbs from moving at will and affecting the normal progress of the test. In addition, the rotating threaded rod can drive the linkage block and the displacement block to move up or down as a whole, so that the distance between the pad and the limiting ring can be adjusted according to the distance between the head and the upper limbs of the experimental mouse, so as to adapt to experimental mice of different sizes.

[0023] 5. The present invention can limit and fix the lower limbs of the experimental mouse by rotating the rotating shaft to tighten the connecting rope, thereby further enhancing the fixation effect of the experimental mouse, and rotating the connecting shaft can drive the rope wheel to rotate forward and reverse, so that the rope wheel applies an upward lifting force or a downward pulling force to the auxiliary bar through the winding rope, thereby adaptively adjusting the height of the mounting block according to the position of the lower limbs of the experimental mouse, so that experimental mice of different sizes can be limited and fixed.

[0024] 6. The present invention can separate multiple medicine injection areas, and then simulate the experimental mice being in an environment of different temperatures for a specified time after taking the medicine, so that the absorption effect of the experimental mice on each component of the Torch Flower Root Tablets at different temperatures can be detected based on the analysis of blood samples; and multiple medicine injection areas are connected, and then multiple experimental mice are infused with Torch Flower Root Tablets with different drug concentrations, so as to detect the absorption effect of the experimental mice on each component of the Torch Flower Root Tablets with different concentrations, which is conducive to the detection diversity of drug components and further enhances the detection effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0026] Figure 1 It is a structural schematic diagram of the present invention.

[0027] Figure 2 It is a schematic diagram of the structure between the head limiting component and the medicine supply component of the present invention.

[0028] Figure 3 The present invention Figure 2 A partial enlarged view of point A.

[0029] Figure 4 The present invention Figure 2 A partial enlarged view of point B.

[0030] Figure 5 It is a schematic diagram of the structure between the upper limb limiting component and the lower limb limiting component of the present invention.

[0031] Figure 6 It is a schematic diagram of the internal structure of the positioning block of the present invention.

[0032] Figure 7 It is a schematic diagram of the structure of the regulating branch chain of the present invention.

[0033] Figure 8 It is a schematic structural diagram of the height-adjustable branch chain of the present invention.

[0034] Fig. 9 The present invention Figure 8 A partial enlarged view of point C.

[0035] Fig.10 It is a structural schematic diagram of the separation and detection unit of the present invention.

[0036] In the figure, 1, vertical bracket; 2, fixing unit; 3, head limit assembly; 31, pad; 32, rubber pad; 33, anti-skid rubber strip; 34, elastic telescopic rod; 35, protective ball; 36, support plate; 37, pull rope; 38, mouth cover; 381, threaded pull rod; 382, ​​positioning frame; 383, fixing nut; 4, upper limb limit assembly; 41, positioning block; 42, limit slide; 43, displacement block; 44, flexible belt; 45, adjustment branch chain; 451, linkage block; 452, threaded rod; 5, lower limb limit assembly; 51, installation Block; 52, actuator plate; 53, height adjustment branch chain; 531, auxiliary strip; 532, connecting shaft; 533, rope pulley; 534, winding rope; 54, supporting plate; 55, rotating shaft; 56, linkage shaft; 57, wire pulley; 58, connecting rope; 59, tightening rope; 6, drug supply component; 61, delivery pump; 62, drug storage barrel; 63, drug delivery tube; 7, separation detection unit; 71, horizontal plate; 72, baffle; 73, cover; 74, temperature controller; 75, air hole; 76, partition; 77, control board; 78, positioning cylinder. DETAILED DESCRIPTION

[0037] The following is combined with Figure 1-Figure 10 Embodiments of the present invention are described in detail.

[0038] The embodiment of the present application discloses a device for detecting the internal and external components of a torch flower root tablet. It should be noted that the device for detecting the internal and external components of a torch flower root tablet of the present application is mainly used in the process of fixing experimental mice, injecting medicine, and drawing blood. In terms of technical effect, the head and limbs of the experimental mice can be fixed in all directions, thereby ensuring the stability of the experimental mice during the injection of torch flower root tablets and blood sampling, and preventing the experimental mice from moving at will and affecting the injection and blood sampling progress. In particular, in the process of fixing the head of the experimental mouse, the mouth of the mouse can be opened on the basis of the head limit fixation, so as to improve the injection effect of the torch flower root tablets, and also It can control the shaking of the experimental mouse's head, so as to expose the blood collection site of the experimental mouse and then fix it in a limited position, which is not only convenient for drug infusion, but also for rapid blood collection, and can effectively improve the detection efficiency. Furthermore, the in vivo and in vitro component detection device of the torch flower root tablets of the present application can also infuse different concentrations of torch flower root tablets into different experimental mice respectively, so as to complete the detection of multiple drug components of different concentrations at the same time, thereby enhancing the detection effect and detection efficiency, so as to detect the absorption effect of the experimental mice on each component in the torch flower root tablets of different concentrations, which is beneficial to the detection diversity of drug components and thus enhances the detection effect.

[0039] Embodiment 1: Reference Figure 1As shown, a device for detecting the internal and external components of a torch flower root piece includes a vertical bracket 1, on which a plurality of fixing units 2 are equidistantly arranged along the length direction, and the fixing units 2 include a head limiting component 3, an upper limb limiting component 4 and a lower limb limiting component 5 which are sequentially distributed from top to bottom.

[0040] Furthermore, in this embodiment, the head limiting assembly 3 includes a pad 31 installed on the side wall of the upright bracket 1 and close to the upper side, the upper limb limiting assembly 4 is arranged on two positioning blocks 41 on the side wall of the upright bracket 1, and the two positioning blocks 41 are both located below the pad 31 and symmetrically distributed along its width direction, and the lower limb limiting assembly 5 includes a mounting block 51 located below the two positioning blocks 41.

[0041] During the specific implementation process, the experimental mouse is first placed on the fixing unit 2 of the vertical bracket 1, and then the head, upper limbs and lower limbs of the experimental mouse are respectively fixed by the head limiting component 3, the upper limb limiting component 4 and the lower limb limiting component 5, so as to ensure the stability of the experimental mouse during the infusion of the torch flower root tablets and blood collection, and to prevent the experimental mouse from moving at will and affecting the progress of the infusion and blood collection; then, the torch flower root tablets of the specified concentration are infused into the experimental mouse's mouth, and blood is collected from the orbital venous plexus of the experimental mouse after the specified time, and then the collected blood samples are tested accordingly, so as to complete the in vivo and in vitro component detection of the torch flower root tablets; in addition, by fixing multiple experimental mice on the vertical bracket 1, different concentrations of the torch flower root tablets can be infused into different experimental mice respectively, so that the detection of multiple drug components of different concentrations can be completed at the same time, thereby enhancing the detection effect and detection efficiency.

[0042] Reference Figure 2 As shown, in order to prevent the inspectors from being scratched when infusing the experimental mice with the torch flower root tablets, based on this, in this embodiment, the experimental mice can be automatically infused with drugs. Specifically, the fixed unit 2 also includes a drug supply component 6 for infusing the experimental mice with the torch flower root tablets. The drug supply component 6 includes a plurality of drug storage barrels 62 installed on the outer wall of the vertical bracket 1 away from the side of the fixed unit 2. The plurality of drug storage barrels 62 correspond to the positions of the plurality of fixed units 2. A delivery pump 61 is installed on the drug storage barrel 62. The output end of the delivery pump 61 is connected to The drug delivery tube 63, one end of the drug delivery tube 63 away from the delivery pump 61 passes through the upright bracket 1 and extends to the pad 31; in the present embodiment, a one-way valve for adding torch flower root tablets is installed on the upper end of the medicine storage barrel 62, and the one-way valve can prevent the torch flower root tablets in the medicine storage barrel 62 from overflowing. In addition, an L-shaped hard tube is installed at one end of the drug delivery tube 63 away from the medicine storage barrel 62 for the convenience of the tester to hold, and the L-shaped hard tube can increase the distance between the tester's hand and the experimental mouse, thereby preventing the tester from being scratched by the experimental mouse.

[0043] During the specific implementation process, after the experimental mouse is fixed in position, the drug delivery tube 63 is inserted into the mouth of the experimental mouse, and then the delivery pump 61 is started. The delivery pump 61 discharges the torch flower root tablets medicine in the medicine storage barrel 62 into the drug delivery tube 63, and the torch flower root tablets medicine is infused into the mouth of the experimental mouse through the drug delivery tube 63, thereby realizing automatic infusion of the drug and avoiding scratches caused by the tester when infusing the drug by himself.

[0044] Reference Figure 2 and Figure 3 As shown, in order to prevent the head of the experimental mouse from shaking during the infusion of the torch flower root tablets and the subsequent blood sampling process and affecting the detection process, it is necessary to limit and fix the head of the experimental mouse accordingly. Based on this, a corresponding head limiting component 3 is provided in this embodiment. Specifically, the head limiting component 3 also includes an arc-shaped groove opened on the side of the pad 31 away from the upright bracket 1, and a rubber pad 32 is installed on the inner wall of the arc-shaped groove. A plurality of anti-slip rubber strips 33 are equidistantly installed along the width direction on the side of the rubber pad 32 away from the pad 31.

[0045] Furthermore, in this embodiment, a plurality of mounting holes are provided on both sides of the inner wall of the arc-shaped groove close to the length direction of the pad 31, and an elastic telescopic rod 34 is arranged in the mounting hole. A protective ball 35 is rotatably installed at one end of the elastic telescopic rod 34 away from the pad 31. The end of the elastic telescopic rod 34 can be shielded by the protective ball 35 to prevent the experimental mouse from colliding with the end of the elastic telescopic rod 34 and being injured during the struggle, thereby affecting the detection accuracy.

[0046] It should be noted that the elastic telescopic rod 34 itself has an ejection elastic force, so that the elastic telescopic rod 34 can apply a thrust with an ejection tendency to the protective ball 35, and the elastic telescopic rod 34 is extended to the longest in the initial state.

[0047] During the specific implementation process, after the experimental mouse is placed on the vertical support 1, its head is placed against the pad 31, so that the head of the experimental mouse is in contact with multiple anti-slip rubber strips 33. The anti-slip rubber strips 33 can increase the friction between the head of the experimental mouse and the head of the experimental mouse, thereby preventing the head of the experimental mouse from moving at will and affecting the infusion of drugs and the blood collection process.

[0048] In addition, when the head of the experimental mouse rests on the pad 31, the elastic telescopic rod 34 that coincides with its contour can be pressed and contracted, so that the other multiple elastic telescopic rods 34 are normally extended, so that the extended elastic telescopic rods 34 are blocked on the outside of the experimental mouse's head, so as to further limit the position of the experimental mouse's head and prevent the experimental mouse's head from shaking randomly, thereby enhancing the stability of the experimental mouse during the detection process.

[0049] Reference Figure 2 and Figure 4As shown, in order to avoid the frequent opening and closing of the mouths of the experimental mice during the infusion of the torch flower root tablets and thus affect the infusion efficiency and infusion amount of the drug, based on this, in this embodiment, the mouths of the experimental mice can also be limited in opening. Specifically, the head limiting assembly 3 also includes two card sleeve groups installed on the side of the pad 31 away from the vertical bracket 1, and the two card sleeve groups are symmetrically distributed along the length direction of the pad 31. The card sleeve groups include two support plates 36 symmetrically distributed along the rubber pad 32, and a pull rope 37 is installed on the support plate 36. A mouthpiece 38 is commonly provided between the two pull ropes 37 of the same card sleeve group.

[0050] Furthermore, in this embodiment, the ends of the two pull ropes 37 on the same side of the two muzzles 38 pass through the support plate 36 and are jointly installed with a threaded pull rod 381. Two positioning frames 382 are symmetrically provided on the outer wall of the pad 31 along the muzzle 38. The positioning frames 382 are installed with fixing nuts 383 which are sleeved on the outer wall of the threaded pull rod 381. The fixing nuts 383 and the threaded pull rod 381 are matched with each other by a threaded connection.

[0051] During the specific implementation process, after the head of the experimental mouse is limited and fixed, the two muzzles 38 are respectively put on the upper and lower jaws of the experimental mouse, and then the threaded pull rod 381 is rotated. Since the fixing nut 383 is always in a fixed state, the threaded pull rod 381 can apply a tightening force to the pull rope 37 during the rotation of the threaded pull rod 381, so that the pull rope 37 tightly buckles the muzzle 38 on the upper and lower jaws of the experimental mouse, thereby further limiting and fixing the head of the experimental mouse, and ensuring that the mouth of the experimental mouse is open, so as to improve the infusion effect of the torch flower root tablets and avoid the outflow of the drug.

[0052] In addition, the muzzle 38 can be controlled to shift by rotating the threaded pull rods 381 on both sides of the pad 31 for different numbers of turns. That is, when the threaded pull rods 381 on either side of the pad 31 are rotated, the threaded pull rods 381 tighten the two muzzles 38 through the pull rope 37, so that the muzzles 38 shift to the side of the threaded pull rods 381, so that the muzzles 38 can drive the head of the experimental mouse to swing adaptively, and then it is convenient to expose the blood collection site of the experimental mouse and then limit and fix it, which not only facilitates the infusion of drugs, but also allows rapid blood collection, which can effectively improve the detection efficiency.

[0053] Reference Figure 5 and Figure 6As shown, in order to ensure that the experimental mouse does not move during the drug infusion and blood collection process, in this embodiment, the upper limbs of the experimental mouse can be correspondingly limited and fixed on the basis of the limited fixation of the head. Specifically, the upper limb limiting assembly 4 includes a limiting slide groove 42 provided on the side of the positioning block 41 away from the upright bracket 1, and a displacement block 43 is slidably connected inside the limiting slide groove 42. The displacement block 43 is provided with an L-shaped groove, and a through hole penetrating the positioning block 41 is provided on the inner bottom wall of the limiting slide groove 42, and the vertical section of the L-shaped groove is connected to the through hole; a flexible belt 44 connected end to end is slidably penetrated in the L-shaped groove, one side of the flexible belt 44 passes through the horizontal section of the L-shaped groove to form a limiting ring, and the other side of the flexible belt 44 passes through the vertical section of the L-shaped groove and extends to the through hole, and an adjusting branch chain 45 is installed between the positioning block 41 and the displacement block 43.

[0054] During the specific implementation process, after the head of the experimental mouse is limited and fixed, its two upper limbs are respectively inserted into two limiting rings, and then the vertical section of the flexible belt 44 is pulled downward to tighten the flexible belt 44 in the horizontal section of the L-shaped groove, thereby reducing the diameter of the limiting ring and making the flexible belt 44 tightened around the outer wall of the upper limb of the experimental mouse. In this way, the upper limb of the experimental mouse can be limited and fixed to prevent it from moving at will and affecting the normal progress of the test.

[0055] Reference Figure 6 and Figure 7 As shown, since the distance between the upper limbs and the head of experimental mice of different sizes is different, an adjustment branch chain 45 that can adaptively adjust the height of the limit ring is provided in this embodiment. Specifically, the adjustment branch chain 45 includes a connecting groove, and the inner wall of the limiting slide groove 42 is symmetrically provided with two connecting grooves along the displacement block 43. The outer wall of the displacement block 43 is equipped with two linkage blocks 451 that are slidably docked in the connecting grooves. Threaded rods 452 are rotatably arranged in the two connecting grooves. The threaded rods 452 pass through the linkage block 451 by means of a threaded connection, and the two threaded rods 452 are connected by a belt drive.

[0056] It should be noted that the two threaded rods 452 on the same positioning block 41 are connected via a belt transmission, so that the two threaded rods 452 can rotate synchronously.

[0057] During the specific implementation process, the threaded rod 452 is rotated, and the threaded rod 452 drives the linkage block 451 and the displacement block 43 to move up or down as a whole, so that the distance between the pad 31 and the limit ring can be adjusted, and then it can be adaptively adjusted according to the distance between the head and upper limbs of the experimental mouse, so as to adapt to experimental mice of different sizes.

[0058] Reference Figure 5As shown, in order to further improve the stability of the experimental mouse, its lower limbs can also be limited and fixed in this embodiment. Specifically, the lower limb limiting assembly 5 includes an actuator plate 52 installed on the side of the upright bracket 1 close to the pad 31, the actuator plate 52 is located below the two positioning blocks 41, and a height adjustment branch chain 53 is installed between the actuator plate 52 and the upright bracket 1. A mounting block 51 and two supporting plates 54 are provided on the side of the actuator plate 52 away from the upright bracket 1. The two supporting plates 54 are symmetrically distributed along the mounting block 51, and a rotating shaft 55 is rotatably provided between the two supporting plates 54 on the same actuator plate 52. The rotating shaft 55 rotates through the mounting block 51, and two adjacent rotating shafts 55 are connected by a linkage shaft 56.

[0059] Furthermore, in this embodiment, two wire wheels 57 are symmetrically sleeved on the outer wall of the rotating shaft 55 along the mounting block 51 and are respectively located below the positioning block 41. A connecting rope 58 is wound around the outer wall of the wire wheel 57. The end of the connecting rope 58 away from the wire wheel 57 passes upward through the through hole and is connected to the flexible belt 44. A tightening rope 59 is also wound around the outer wall of the rotating shaft 55. The tightening rope 59 has a pulling ring on the side away from the execution plate 52.

[0060] During the specific implementation process, after the head and upper limbs of the experimental mouse are limited and fixed, the lower limbs of the experimental mouse are inserted into the retracting ring, and then the linkage shaft 56 is rotated. The linkage shaft 56 drives the multiple rotating shafts 55 to rotate synchronously. The rotating shaft 55 tightens the connecting rope 58, so that the pull rope 37 reduces the inner diameter of the retracting ring, so as to facilitate the limited fixation of the lower limbs of the experimental mouse, thereby further enhancing the fixation effect of the experimental mouse; at the same time, during the rotation of the rotating shaft 55, the connecting rope 58 is reeled in by the wire wheel 57, so that the connecting rope 58 applies tension to the flexible belt 44, and then the lower limbs of the experimental mouse are limited and fixed while the upper limbs are limited and fixed, thereby improving convenience.

[0061] Reference Figure 5 , Figure 8 and Fig. 9 As shown, in order to limit and fix the lower limbs of experimental mice of different sizes, it is necessary to adjust the height of the mounting block 51. Based on this, in the present embodiment, the height adjustment branch chain 53 includes a sliding groove, and the side of the vertical bracket 1 close to the execution plate 52 has a plurality of linkage groups corresponding to the position of the execution plate 52, and each linkage group includes two sliding grooves symmetrically distributed along the length direction of the execution plate 52, and the side of the execution plate 52 close to the vertical bracket 1 is equipped with two auxiliary strips 531 that are slidably docked in the sliding groove; the side of the vertical bracket 1 away from the execution plate 52 is provided with a connecting shaft 532 through a fixed frame for rotation, and the outer wall of the connecting shaft 532 is provided with a plurality of rope pulleys 533 corresponding to the position of the execution plate 52, and the upper and lower ends of the auxiliary strip 531 are installed with winding ropes 534, and the ends of the two winding ropes 534 away from the auxiliary strip 531 pass through the vertical bracket 1 and are wound around the rope pulley 533 together.

[0062] It should be noted that a damping ring is provided between the connecting shaft 532 and the fixing frame, and the damping ring can limit the rotation direction of the connecting shaft 532, so that the connecting shaft 532 can be limited after rotation and will not rotate at will.

[0063] During the specific implementation process, the connecting shaft 532 is rotated, and the connecting shaft 532 drives the rope pulley 533 to rotate forward or reversely. When the rope pulley 533 rotates forward, the winding rope 534 on the upper side applies an upward lifting force to the auxiliary bar 531, so that the auxiliary bar 531 drives the execution plate 52, the mounting block 51 and the tightening rope 59 to move upward as a whole; when the rope pulley 533 rotates reversely, the winding rope wheel 533 on the lower side moves the auxiliary bar 531, the execution plate 52, the mounting block 51 and the tightening rope 59 downward as a whole, so that the height of the mounting block 51 can be adaptively adjusted according to the position of the lower limbs of the experimental mouse, so that experimental mice of different sizes can be limited and fixed.

[0064] It should be further explained that after adjusting the height of the actuator plate 52 and the mounting block 51 , the connecting shaft 532 is adaptively rotated so that the rope wheel 533 adaptively reels and unwinds the winding rope 534 according to the distance between the mounting block 51 and the positioning block 41 .

[0065] Embodiment 2: Reference Fig.10 As shown, on the basis of Example 1, in order to further enhance the detection effect, and after the drug is infused into the body of the experimental mouse, whether the experimental mouse is in an environment of different temperatures that affects the absorption effect of each component of the drug, based on this, a separation detection unit 7 is also provided in this embodiment, and the separation detection unit 7 includes a horizontal plate 71 installed at the bottom of the vertical bracket 1 close to the side of the fixed unit 2, and a plurality of baffles 72 are installed between the vertical bracket 1 and the horizontal plate 71 and are spaced apart from the fixed units 2. The plurality of baffles 72 can separate the plurality of fixed units 2, and a plurality of drug injection areas are formed between the baffles 72, the horizontal plate 71 and the vertical bracket 1. A cover 73 for closing the drug injection area is rotatably installed on the horizontal plate 71, and a temperature controller 74 for adjusting the temperature in the drug injection area is installed on the cover 73.

[0066] Furthermore, in the present embodiment, an air-connecting hole 75 for connecting the two medicine-filling areas is provided on the baffle 72 between two adjacent fixed units 2, and a receiving groove is provided through the upper end of the baffle 72, which is connected to the air-connecting hole 75, and a partition 76 is slidably provided in the receiving groove, and a control board 77 is commonly provided at the upper ends of the plurality of partitions 76, and a plurality of mounting grooves staggered with the positions of the partitions 76 are provided at the upper end of the vertical bracket 1, and a positioning cylinder 78 is provided in the mounting groove, and the top of the telescopic end of the positioning cylinder 78 is connected to the control board 77.

[0067] In the initial state, the telescopic end of the positioning cylinder 78 is in an extended state to facilitate lifting the control plate 77 upward, and the control plate 77 drives the partition plate 76 to move up to the highest position. At this time, multiple medicine filling areas are connected through the string holes 75.

[0068] During the specific implementation process, the cover 73 is opened and rotated downward along the connection between the cover 73 and the horizontal plate 71 to open the medicine filling area and fix the experimental mouse in a limited position, and then the medicine is injected into the mouth of the experimental mouse. Then, the cover 73 is closed and the positioning cylinder 78 is started. The telescopic end of the positioning cylinder 78 drives the control plate 77 and the partition 76 to move downward, so that the partition 76 separates the multiple medicine filling areas; then, the temperature controller 74 is started to make the temperatures in the multiple medicine filling areas different, thereby simulating the experimental mouse being in an environment of different temperatures for a specified time after taking the medicine, and then blood is collected from different experimental mice. According to the analysis of the blood samples, it is convenient to detect the absorption effect of the experimental mice on the various components in the Torch Flower Root Tablets at different temperatures, which is conducive to the detection of diversity of drug components.

[0069] In addition, by driving the control plate 77 and the partition 76 upward to the initial state through the positioning cylinder 78, the temperatures of multiple drug filling areas are made the same. At this time, multiple experimental mice can be infused with torch flower root tablets of different drug concentrations to detect the absorption effect of the experimental mice on the various components in the torch flower root tablets of different concentrations, thereby enhancing the detection effect.

[0070] In addition, the present invention also provides a method for detecting the internal and external components of the torch flower root slice, comprising the following steps: S1: Placing the experimental mouse: First, place the experimental mouse on the vertical support 1 , and then place its head against the pad 31 , so that the head of the experimental mouse contacts the plurality of anti-slip rubber strips 33 .

[0071] S2: Fixing the head of the experimental mouse: The head of the experimental mouse can be limited by the anti-slip rubber strip 33 and multiple elastic telescopic rods 34 to prevent its head from shaking at will. Then, the two muzzles 38 are respectively put on the upper and lower jaws of the experimental mouse, and the threaded pull rod 381 is rotated to apply a tightening force to the pull rope 37, so that the pull rope 37 tightly buckles the muzzle 38 on the upper and lower jaws of the experimental mouse, thereby further limiting and fixing the head of the experimental mouse and ensuring that the mouth of the experimental mouse is open, so as to improve the infusion effect of the Torch Flower Root Tablets.

[0072] In addition, by controlling the offset of the muzzle 38, the head of the experimental mouse can be driven to swing adaptively, thereby facilitating the exposure of the blood collection site of the experimental mouse and then limiting and fixing it. This not only facilitates the infusion of drugs, but also allows for rapid blood collection, which can effectively improve the detection efficiency.

[0073] S3: Fixing the limbs of the experimental mouse: insert the two upper limbs of the experimental mouse into the two limiting rings respectively, and insert the two lower limbs into the retracting ring, then rotate the linkage shaft 56, the linkage shaft 56 drives the multiple rotating shafts 55 to rotate synchronously, the rotating shaft 55 tightens the connecting rope 58, so that the pull rope 37 reduces the inner diameter of the retracting ring, so as to facilitate the limiting and fixing of the lower limbs of the experimental mouse; at the same time, the connecting rope 58 is reeled in by the wire wheel 57 during the rotation of the rotating shaft 55, and the connecting rope 58 applies tension to the flexible belt 44, so that the flexible belt 44 is tightened and the upper limbs of the experimental mouse are limited and fixed.

[0074] In addition, the rotating threaded rod 452 drives the linkage block 451 and the displacement block 43 to move up or down as a whole, so that the distance between the pad 31 and the limiting ring can be adjusted, and then it can be adaptively adjusted according to the distance between the head and upper limbs of the experimental mouse, so as to adapt to experimental mice of different sizes.

[0075] Furthermore, rotating the connecting shaft 532 can drive the rope wheel 533 to rotate forward and reverse, so that the rope wheel 533 applies an upward lifting force or a downward pulling force to the auxiliary bar 531 through the winding rope 534, thereby adaptively adjusting the height of the mounting block 51 according to the position of the lower limbs of the experimental mouse, thereby being able to limit and fix experimental mice of different sizes.

[0076] S4: Infusing the experimental mouse with medicine and taking blood for testing: After the experimental mouse is fixed in position, insert the drug delivery tube 63 into the mouth of the experimental mouse, and then start the delivery pump 61. The delivery pump 61 discharges the torch flower root tablets in the medicine storage barrel 62 into the drug delivery tube 63, and then the torch flower root tablets are infused into the mouth of the experimental mouse through the drug delivery tube 63, thereby realizing automatic infusion of the drug; after a specified time, blood is collected from the orbital venous plexus of the experimental mouse, and then the collected blood sample is subjected to corresponding testing, thereby completing the in vivo and in vitro component testing of the torch flower root tablets.

[0077] It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention.

[0078] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A device for detecting the internal and external components of a torch flower root piece, comprising a vertical support (1), characterized in that: A plurality of fixing units (2) are equidistantly arranged on the vertical support (1) along the length direction, and the fixing units (2) include a head limiting component (3), an upper limb limiting component (4) and a lower limb limiting component (5) which are sequentially distributed from top to bottom, wherein: The head limiting assembly (3) comprises a pad (31) mounted on the side wall of the vertical support (1) and close to the upper side; the upper limb limiting assembly (4) is provided with two positioning blocks (41) on the side wall of the vertical support (1); the two positioning blocks (41) are both located below the pad (31) and are symmetrically distributed along the width direction thereof; and the lower limb limiting assembly (5) comprises a mounting block (51) located below the two positioning blocks (41).

2. A device for detecting internal and external components of a torch flower root piece according to claim 1, characterized in that: The fixed unit (2) also includes a drug supply component (6) for infusing the torch flower root tablets into the experimental mice. The drug supply component (6) includes a plurality of drug storage barrels (62) installed on the outer wall of the vertical support (1) away from the fixed unit (2). The plurality of drug storage barrels (62) correspond to the positions of the plurality of fixed units (2). A delivery pump (61) is installed on the drug storage barrel (62). The output end of the delivery pump (61) is connected to a drug delivery tube (63). The end of the drug delivery tube (63) away from the delivery pump (61) passes through the vertical support (1) and extends to the pad (31).

3. A device for detecting internal and external components of a torch flower root piece according to claim 1, characterized in that: The head limiting assembly (3) further comprises an arc-shaped groove formed on a side of the backing plate (31) away from the vertical bracket (1), a rubber pad (32) being installed on the inner wall of the arc-shaped groove, and a plurality of anti-slip rubber strips (33) being installed at equal distances along the width direction on the side of the rubber pad (32) away from the backing plate (31); A plurality of mounting holes are provided on both sides of the inner wall of the arc-shaped groove in the length direction close to the backing plate (31), and elastic telescopic rods (34) are arranged in the mounting holes. A protective ball (35) is rotatably mounted on one end of the elastic telescopic rod (34) away from the backing plate (31).

4. A device for detecting internal and external components of a torch flower root piece according to claim 3, characterized in that: The head limit assembly (3) further comprises two ferrule groups mounted on a side of the pad (31) away from the vertical bracket (1), the two ferrule groups being symmetrically distributed along the length direction of the pad (31), each ferrule group comprising two support plates (36) symmetrically distributed along the rubber pad (32), a pull rope (37) being mounted on the support plate (36), and a mouthpiece (38) being provided between the two pull ropes (37) of the same ferrule group.

5. A device for detecting internal and external components of a torch flower root piece according to claim 4, characterized in that: The ends of the two pull ropes (37) on the same side of the two mouth sleeves (38) pass through the support plate (36) and are jointly installed with a threaded pull rod (381). Two positioning frames (382) are symmetrically arranged along the outer wall of the backing plate (31) along the mouth sleeve (38). The positioning frames (382) are installed with a fixing nut (383) sleeved on the outer wall of the threaded pull rod (381). The fixing nut (383) and the threaded pull rod (381) are matched with each other by a threaded connection.

6. A device for detecting internal and external components of a torch flower root piece according to claim 1, characterized in that: The upper limb limiting assembly (4) comprises a limiting slide groove (42) provided on a side of the positioning block (41) away from the vertical support (1), a displacement block (43) being slidably connected inside the limiting slide groove (42), an L-shaped groove being provided on the displacement block (43), a through hole penetrating the positioning block (41) being provided on the inner bottom wall of the limiting slide groove (42), and a vertical section of the L-shaped groove being connected to the through hole; A flexible belt (44) connected end to end is slidably provided in the L-shaped groove, one side of the flexible belt (44) passes through the horizontal section of the L-shaped groove to form a limit ring, and the other side of the flexible belt (44) passes through the vertical section of the L-shaped groove and extends into the through hole. An adjustment branch chain (45) is installed between the positioning block (41) and the displacement block (43).

7. A device for detecting internal and external components of a torch flower root piece according to claim 6, characterized in that: The adjusting branch chain (45) comprises a connecting groove, the inner wall of the limiting sliding groove (42) is symmetrically provided with two connecting grooves along the displacement block (43), the outer wall of the displacement block (43) is provided with two linkage blocks (451) slidably docked in the connecting grooves, and threaded rods (452) are rotatably arranged in the two connecting grooves, and the threaded rods (452) pass through the linkage blocks (451) in a threaded connection manner.

8. The device for detecting internal and external components of a torch flower root piece according to claim 1, characterized in that: The lower limb limiting assembly (5) further comprises an actuator plate (52) mounted on a side of the vertical support (1) close to the pad (31), the actuator plate (52) being located below the two positioning blocks (41), a height adjustment branch chain (53) being installed between the actuator plate (52) and the vertical support (1), a mounting block (51) and two supporting plates (54) being arranged on a side of the actuator plate (52) away from the vertical support (1), the two supporting plates (54) being symmetrically distributed along the mounting block (51), a rotating shaft (55) being rotatably arranged between the two supporting plates (54) on the same actuator plate (52), the rotating shaft (55) rotating through the mounting block (51), and two adjacent rotating shafts (55) being connected via a linkage shaft (56); Two wire wheels (57) are symmetrically sleeved on the outer wall of the rotating shaft (55) along the mounting block (51), and are respectively located below the positioning block (41). A connecting rope (58) is wound around the outer wall of the wire wheel (57). One end of the connecting rope (58) away from the wire wheel (57) passes upward through the through hole and is connected to the flexible belt (44). A tightening rope (59) is also wound around the outer wall of the rotating shaft (55). The tightening rope (59) has a retracting ring on one side away from the execution plate (52).

9. A device for detecting internal and external components of a torch flower root piece according to claim 8, characterized in that: The height adjustment branch chain (53) comprises a sliding groove, and a side of the vertical support (1) close to the execution plate (52) comprises a plurality of linkage groups corresponding to the position of the execution plate (52), each linkage group comprises two sliding grooves symmetrically distributed along the length direction of the execution plate (52), and a side of the execution plate (52) close to the vertical support (1) is provided with two auxiliary bars (531) slidably docked in the sliding grooves; A connecting shaft (532) is rotatably provided on a side of the vertical support (1) away from the actuating plate (52) via a fixing frame, and a plurality of rope pulleys (533) corresponding to the positions of the actuating plate (52) are sleeved on the outer wall of the connecting shaft (532), and winding ropes (534) are installed at both upper and lower ends of the auxiliary strip (531), and ends of the two winding ropes (534) away from the auxiliary strip (531) pass through the vertical support (1) and are wound around the rope pulleys (533) together.

10. A method for detecting internal and external components of a torch flower root slice, comprising a device for detecting internal and external components of a torch flower root slice as claimed in any one of claims 1 to 9, characterized in that: The detection method includes the following steps: S1: Placement of experimental mice: First, place the experimental mice on the fixing unit (2) of the vertical support (1); S2: Fixing the head of the experimental mouse: The head of the experimental mouse is fixed by the head limiting component (3); S3: Fixing the limbs of the experimental mouse: The limbs of the experimental mouse are fixed by the upper limb limiting assembly (4) and the lower limb limiting assembly (5); S4: Instilling medicine into experimental mice and collecting blood for testing: Infuse the experimental mice with a specified concentration of Torch Flower Root Tablets through their mouths. After a specified period of time, collect blood from the experimental mice's orbital venous plexus and conduct corresponding analysis and testing on the collected blood samples, thereby completing the in vivo and in vitro component testing of the Torch Flower Root Tablets.

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