Water channel composition as well as preparation method and application thereof

Abstract

The invention provides a water channel composition. The water channel composition is prepared from 60 to 90 parts of deionized water, 2 to 6 parts of urea, 1 to 3 parts of yeast extract, 1 to 3 parts of trehalose, 0.3 to 0.6 part of inositol, 0.1 to 0.3 part of taurine, 0.5 to 1.5 parts of betaine, 1.5 to 2.5 parts of phenoxyethanol and 1 to 4 parts of potassium sorbate. When the prepared water channel composition is added into cosmetics, the percutaneous moisture loss can be obviously reduced, the moisturizing effect is improved, and the barrier function of the skin is enhanced while powerful skin moisturizing is promoted.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetic preparation, and specifically relates to a water channel composition and a preparation method and application thereof. Background Art

[0002] Water is essential to human health because it helps digestion, proper metabolism and circulation in the body. Proper hydration is also the most important factor for healthy and youthful skin. The epidermis, as the outer layer of the skin, is exposed to the environment for a long time and is affected by various factors such as temperature changes, dry air, humidity and pollution. It is easy to damage the skin's barrier protection function, leading to skin moisture loss, dry skin, and even wrinkles.

[0003] Optimal epidermal hydration increases cellular metabolism, allowing cells to function more efficiently. By increasing cellular efficiency, certain cellular activities, such as protein production, also increase. Proteins such as proteoglycans are essential for epidermal integrity. Increasing intracellular hydration not only improves skin, but also reduces the appearance of fine lines and wrinkles. Water is essential for life and our health and skin. Well-hydrated skin looks clearer, younger, plumper, and glows from within.

[0004] Aquaporins are proteins that have designated water-balancing channels in our skin cells. Epidermal moisturizing can be improved by increasing the activity of aquaporin-3 in the dermis. Aquaporin-3 is synthesized by human keratinocytes and enhances skin hydration by increasing osmosis between the dermis and keratin. The function of aquaporin-3 is to move water against the concentration gradient to areas where water is scarce.

[0005] However, the moisturizing effect obtained by conventional products is only short-term in most cases, and usually does not have a deep and long-term good effect, that is, it does not moisturize deeper skin layers. Therefore, it is of great significance to develop a water channel composition to increase the moisturizing time and effect. Summary of the invention

[0006] The purpose of the present invention is to provide a water channel composition and a preparation method and application thereof. By using the synergistic effect of urea, yeast extract, trehalose, inositol, taurine, betaine and other ingredients, a special water channel composition is studied and applied to moisturizing products, and the moisturizing effect and maintenance time are significantly improved.

[0007] In order to achieve the above object, the present invention provides the following technical solutions: A water channel composition comprises 60-90 parts of deionized water, 2-6 parts of urea, 1-3 parts of yeast extract, 1-3 parts of trehalose, 0.3-0.6 parts of inositol, 0.1-0.3 parts of taurine, 0.5-1.5 parts of betaine, 1.5-2.5 parts of phenoxyethanol and 1-4 parts of potassium sorbate.

[0008] Furthermore, the yeast extract is obtained by sequentially treating yeast cell residues with enzymes and ultrafiltration.

[0009] Furthermore, the enzyme is a combination of papain and alkaline protease or a combination of cell wall-breaking enzyme and yeast extract enzyme.

[0010] Furthermore, the ultrafiltration uses an organic ultrafiltration membrane, and the molecular weight cutoff of the organic ultrafiltration membrane is 5-20kDa.

[0011] Furthermore, the ultrafiltration pressure is 0.5-1.5 MPa, the temperature is 20-30° C., and the time is 10-15 min.

[0012] A method for preparing a water channel composition comprises the following steps: S1: Preparation of yeast extract; S2: Mix 60-90 parts of deionized water, 1-3 parts of yeast extract and 0.3-0.6 parts of inositol, and stir at 85-95°C for 30-40 minutes; S3: Lower the temperature to 45-55°C, add 1-3 parts of trehalose, 0.1-0.3 parts of taurine and 0.5-1.5 parts of betaine, stir and react for 15-25 minutes, and keep warm for 5-10 minutes; S4: lowering the temperature to 30-35° C., adding 1.5-2.5 parts of phenoxyethanol and 1-4 parts of potassium sorbate, stirring and reacting for 25-35 minutes, and obtaining a water channel composition.

[0013] Furthermore, step S1 is specifically as follows: preparing a yeast cell residue suspension with a mass concentration of 25%, shaking at 250 r / min for 5-10 min at a temperature of 50° C. and a pH of 6.5; adding an enzyme accounting for 1% of the mass fraction of the suspension, reacting for 8 hours, centrifuging at 15000 rpm for 15 minutes to remove the supernatant; ultrafiltration at a pressure of 1.2 MPa and a temperature of 25° C. using an organic membrane with a molecular weight cutoff of 10 kDa for 12 minutes to obtain a yeast extract.

[0014] Furthermore, the yeast is one or a mixture of Pichia pastoris, Saccharomyces cerevisiae or Saccharomyces.

[0015] Furthermore, the yeast is a mixture of brewer's yeast and saccharomyces cerevisiae, and the mass ratio of the two is 1:1.2-1.8.

[0016] Application of any of the above prepared water channel compositions in hydrating, moisturizing, skin and hair moisturizing products.

[0017] Trehalose is a naturally occurring disaccharide composed of two glucose molecules connected by an α,α-1,1-glycosidic bond. Trehalose has a wide range of applications in many fields, including food industry, medicine, cosmetics and biotechnology, due to its unique physical and chemical properties and biological functions.

[0018] Yeast Extract is a multifunctional food ingredient and biological product extracted from yeast cells, which is rich in nutrients and has multiple functions. Yeast Extract has a wide range of applications in the food industry, feed industry, medicine and biotechnology due to its unique flavor, nutritional value and functionality.

[0019] Inositol is a cyclohexanehexol, also known as cyclohexanehexol or vitamin B8, although it is not a vitamin in the traditional sense. Inositol is widely present in animal and plant cells in nature, especially in fruits, beans, grains and liver. Inositol has multiple physiological functions in the human body, including participation in phospholipid metabolism, signal transduction, neurotransmission and blood sugar regulation.

[0020] Compared with the prior art, the advantages and beneficial effects of the present invention are: The present invention uses urea, yeast extract, trehalose, inositol, taurine, betaine, phenoxyethanol and potassium sorbate to prepare a water channel composition, which uses urea, trehalose, inositol and other ingredients to synergistically moisturize and accelerate the shedding of aged keratin; yeast extract, betaine and taurine are used to regulate the flow of water in cells and quickly provide energy supplement to the skin. The composition has a deep moisturizing function for the skin, has a stronger moisturizing and water-locking effect, and can effectively prevent dryness of the skin and mucous membranes. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is the average TEWL reading; Figure 2 It is transepidermal water loss; Figure 3 is an average hydration reading; Figure 4 The percentage of moisturizing changes. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] The sources of raw materials used in the present invention are as follows: Urea, trehalose, inositol, taurine, betaine, phenoxyethanol, and potassium sorbate were from Shanghai Yuanye Biotechnology Co., Ltd., item numbers S30375, S11052, S11074, S20155, S18046, B67323, and S11114; papain, alkaline protease, wall-breaking enzyme, and yeast extraction enzyme were from Hefei Tanrun Biotechnology Co., Ltd.; Pichia pastoris was from Shanghai Yubo Biotechnology Co., Ltd., item number YB4000A; brewer's yeast was from Jiangsu Jiujia Biotechnology Co., Ltd., item number 2153; and yeast was from Henan Yinian Biotechnology Co., Ltd., item number 66.

[0024] Example 1 This embodiment provides a method for preparing a water channel composition, comprising the following steps: S1: A mixture of brewer's yeast and yeast in a mass ratio of 1:1.2 was used as the yeast raw material, and a yeast cell residue suspension with a mass concentration of 25% was prepared. The suspension was shaken at 250 r / min for 5 min at a temperature of 50°C and a pH of 6.5. An enzyme accounting for 1% of the mass fraction of the suspension was added, and the reaction was carried out for 8 h. The supernatant was removed by centrifugation at 15000 rpm for 15 min. The yeast extract was obtained by ultrafiltration at a pressure of 0.5 MPa and a temperature of 20°C using an organic membrane with a molecular weight cutoff of 5 kDa for 10 min. S2: 60 g of deionized water, 1 g of yeast extract and 0.3 g of inositol were mixed and stirred at 85 °C and 600 r / min for 30 min; S3: Lower the temperature to 45°C, add 1 g trehalose, 0.1 g taurine and 0.5 parts betaine, stir and react for 15 min, and keep warm for 5 min; S4: The temperature was lowered to 30° C., and 1.5 g of phenoxyethanol and 1 g of potassium sorbate were added, and the mixture was stirred for reaction for 25 min to obtain a water channel composition.

[0025] Example 2 This embodiment provides a method for preparing a water channel composition, comprising the following steps:

[0026] S1: A mixture of brewer's yeast and yeast in a mass ratio of 1:1.8 was used as the yeast raw material, and a yeast cell residue suspension with a mass concentration of 25% was prepared. The suspension was shaken at 250 r / min for 10 min at a temperature of 50°C and a pH of 6.5. An enzyme accounting for 1% of the mass fraction of the suspension was added, and the reaction was carried out for 8 h. The supernatant was removed by centrifugation at 15000 rpm for 15 min. The yeast extract was obtained by ultrafiltration at a pressure of 1.5 MPa and a temperature of 30°C using an organic membrane with a molecular weight cutoff of 20 kDa for 15 min. S2: 90 g of deionized water, 3 g of yeast extract and 0.6 g of inositol were mixed and stirred at 95 °C and 600 r / min for 40 min; S3: Lower the temperature to 55°C, add 3 g trehalose, 0.3 g taurine and 1.5 parts betaine, stir and react for 25 min, and keep warm for 10 min; S4: The temperature was lowered to 35° C., and 2.5 g of phenoxyethanol and 4 g of potassium sorbate were added, and the mixture was stirred for reaction for 35 min to obtain a water channel composition.

[0027] Example 3 This embodiment provides a method for preparing a water channel composition, comprising the following steps: S1: A mixture of brewer's yeast and yeast in a mass ratio of 1:1.5 was used as the yeast raw material, and a yeast cell residue suspension with a mass concentration of 25% was prepared. The suspension was shaken at 250 r / min for 8 min at a temperature of 50°C and a pH of 6.5. An enzyme accounting for 1% of the mass fraction of the suspension was added, and the reaction was carried out for 8 h. The supernatant was removed by centrifugation at 15000 rpm for 15 min. The yeast extract was obtained by ultrafiltration at a pressure of 1.2 MPa and a temperature of 25°C using an organic membrane with a molecular weight cutoff of 10 kDa for 12 min. S2: 80 g of deionized water, 2 g of yeast extract and 0.5 g of inositol were mixed and stirred at 90 °C and 600 r / min for 35 min; S3: Lower the temperature to 52°C, add 2 g trehalose, 0.2 g taurine and 1.2 parts betaine, stir and react for 22 min, and keep warm for 7 min; S4: The temperature was lowered to 32° C., and 2.2 g of phenoxyethanol and 3 g of potassium sorbate were added, and the mixture was stirred for reaction for 30 min to obtain a water channel composition.

[0028] The water channel composition obtained in this example is applied in moisturizing product applications.

[0029] Comparative Example 1 Commercially available basic cream.

[0030] Comparative Example 2 The product without adding the water channel composition of Example 3. That is, 12 g of polyethylene glycol ether, 3 g of acrylonitrile, 5 g of citric acid, and 60 g of water were taken, and stirred at 40° C. and 2000 rpm for 30 min to obtain the product.

[0031] Comparative Example 3 The product of the water channel composition of Example 3 was added, that is, 12 g of polyethylene glycol ether, 3 g of acrylonitrile, 5 g of citric acid, 0.4 g of the water channel composition of Example 3, and 60 g of water were taken, and stirred at 40° C. and 2000 rpm for 30 min to obtain the product.

[0032] Performance Test: Invite 33 male / female subjects aged between 23 and 45 to participate in the test by applying the films of the products of comparative examples 1-3. The average readings of TEWL of comparative examples 1-3, as well as the readings of the reduction of water loss over time, the average moisturizing readings and the change rate of moisturizing percentage were tested at 0min, 15min, 8h and 24h after application. Figure 1-4 .

[0033] The basic lotion is: from Figure 1 and Figure 2 It can be seen that the moisturizing product added with the water channel composition of Example 3 can effectively reduce transepidermal water loss, and the effect is better over time, and can promote the moisturizing effect when added to cosmetics. Compared with Comparative Example 1 and Comparative Example 2, the transepidermal water loss after 24 hours is reduced by 25.82% and 39.47%, respectively. It shows that the water channel composition prepared by the present invention can significantly reduce TEWL, thereby maintaining moisture.

[0034] from Figure 3 and Figure 4 It can be seen that the moisture content of the product of Comparative Example 3 with the addition of the water channel composition increased by 126.51% after 15 minutes and increased by 42.94% after 24 hours compared with the untreated Comparative Example 1; compared with the basic lotion of Comparative Example 2, the moisturizing effect of the lotion of Comparative Example 3 increased by 13.06% after 15 minutes and increased by 21.72% after 24 hours. The results show that Comparative Example 3 with the addition of the water channel composition can increase skin moisturizing to a greater extent.

[0035] However, since the water channel composition of the present invention is not added to Comparative Examples 1-2, the corresponding performance tests are obviously worse than those of the embodiments. The above experimental results further prove the importance of the technical solution defined in the present invention to its technical effect.

[0036] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A water channel composition, characterized in that The invention comprises 60-90 parts of deionized water, 2-6 parts of urea, 1-3 parts of yeast extract, 1-3 parts of trehalose, 0.3-0.6 parts of inositol, 0.1-0.3 parts of taurine, 0.5-1.5 parts of betaine, 1.5-2.5 parts of phenoxyethanol and 1-4 parts of potassium sorbate.

2. A water channel composition according to claim 1, characterized in that: The yeast extract is obtained by sequentially treating yeast cell residues with enzymes, ultrafiltration, and removing endotoxins.

3. A water channel composition according to claim 2, characterized in that: The enzyme is a combination of papain and alkaline protease or a combination of cell wall breaking enzyme and yeast extract enzyme.

4. A water channel composition according to claim 2, characterized in that: The ultrafiltration uses an organic ultrafiltration membrane, and the molecular weight cutoff of the organic ultrafiltration membrane is 5-20 kDa.

5. The water channel composition according to claim 2, characterized in that: The ultrafiltration pressure is 0.5-1.5 MPa, the temperature is 20-30° C., and the time is 10-15 min.

6. A method for preparing a water channel composition, characterized in that: The steps include: S1: Preparation of yeast extract; S2: Mix 60-90 parts of deionized water, 1-3 parts of yeast extract and 0.3-0.6 parts of inositol, and stir at 85-95°C for 30-40 minutes; S3: Lower the temperature to 45-55°C, add 1-3 parts of trehalose, 0.1-0.3 parts of taurine and 0.5-1.5 parts of betaine, stir and react for 15-25 minutes, and keep warm for 5-10 minutes; S4: lowering the temperature to 30-35° C., adding 1.5-2.5 parts of phenoxyethanol and 1-4 parts of potassium sorbate, stirring and reacting for 25-35 minutes, and obtaining a water channel composition.

7. The method for preparing a water channel composition according to claim 6, characterized in that: Step S1 specifically comprises: preparing a yeast cell residue suspension with a mass concentration of 25%, shaking at 250 r / min for 5-10 min at a temperature of 50° C. and a pH of 6.5; adding an enzyme accounting for 1% of the mass fraction of the suspension, reacting for 8 h, centrifuging at 15000 rpm for 15 min to remove the supernatant; ultrafiltration at a pressure of 1.2 MPa and a temperature of 25° C. using an organic membrane with a molecular weight cutoff of 10 kDa for 12 min to obtain a yeast extract.

8. The method for preparing a water channel composition according to claim 6, characterized in that: The yeast is one or a mixture of Pichia pastoris, Saccharomyces cerevisiae or Saccharomyces cerevisiae.

9. The method for preparing a water channel composition according to claim 8, characterized in that: The yeast is a mixture of brewer's yeast and saccharomyces cerevisiae, with a mass ratio of 1:1.2-1.

8.

10. Use of the water channel composition prepared according to any one of claims 1 to 9 in hydrating, moisturizing, skin and hair moisturizing products.

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