Anti-hair-loss and oil-control composition suitable for Asian men and application of anti-hair-loss and oil-control composition

Abstract

The invention discloses an anti-hair-loss and oil-control composition suitable for Asian men and application of the anti-hair-loss and oil-control composition, and relates to the technical field of cosmetics. The composition disclosed by the invention is prepared from the following components in parts by weight: 0.5 to 10 parts of melissa officinalis extract, 0.05 to 3 parts of gorgonian extract, 0.02 to 3 parts of cypress leaf extract and 0.05 to 0.8 part of black willow bark extract. According to the composition, the melissa officinalis extract, the gorgonian extract, the hinokii leaf extract and the black willow bark extract are combined to have a synergistic interaction effect, and the composition formed by the components through specific compatibility can improve the alopecia problem of sensitive skin from multiple dimensions of comprehensive emotion regulation, cell autophagy activation, oil control and soothing; the anti-alopecia composition integrates anti-alopecia components on the physiological level and emotion relieving components on the psychological level, realizes double intervention on the alopecia problem, selects plant components, and is more suitable for being used by male Asian people with sensitive skin.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to an anti-hair loss and oil control composition suitable for Asian men and application thereof. Background Art

[0002] As the pace of life continues to accelerate and the pressure of life and work increases, hair loss has become a common problem faced by the social population, and it is showing a trend of becoming younger. The scalp is a part of the skin and has a similar structure to the facial skin, but the scalp is more fragile than the facial skin. Compared with the cheeks, the scalp is thinner but has more sebaceous glands, which means that the scalp is more sensitive and secretes more oil than the cheeks. Therefore, the scalp should be taken care of more, and the care products used should pay more attention to sensitivity and other issues.

[0003] In the field of men's anti-hair loss products, the existing technologies on the market mostly rely on chemical synthetic ingredients. Although these products can alleviate hair loss problems to a certain extent, they often ignore the special needs of sensitive skin. In addition, hair loss problems are often related to emotional stress, especially for men with sensitive skin. Existing anti-hair loss products usually only focus on a single effect, such as oil control or promoting hair growth, but lack comprehensive consideration of multiple effects such as emotional regulation, cell autophagy activation and soothing. It is of great significance to develop an anti-hair loss product that can act on both the physiological and psychological levels.

[0004] It can be speculated that adding mood-regulating ingredients to anti-hair loss products, in combination with oil control, cell autophagy activation, anti-inflammatory and soothing ingredients, can effectively improve men's hair loss caused by excessive stress. In addition, the selected active ingredients are all plant-based, which are more suitable for people with sensitive skin.

[0005] Existing anti-hair loss products have a single function. Most products only address the problem of hair loss, ignoring other important factors such as mood regulation and skin soothing. Existing anti-hair loss products add a variety of functional ingredients to improve their functions, ignoring the needs of Asian men with sensitive skin. Therefore, seeking products suitable for anti-hair loss and oil control for Asian men with sensitive skin has become a technical problem that needs to be solved urgently in this field. Summary of the invention

[0006] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide an anti-hair loss and oil-control composition and application suitable for Asian men, which can improve the hair loss problem of sensitive skin of Asian men from multiple dimensions such as comprehensive emotional regulation, activation of cell autophagy, oil control, and soothing; the anti-hair loss composition combines the anti-hair loss ingredients at the physiological level and the emotional soothing ingredients at the psychological level, achieving a dual intervention in the hair loss problem, and all of them are plant ingredients, which is more suitable for use by Asian men with sensitive skin.

[0007] To achieve the above object, the technical solution adopted by the present invention is:

[0008] In a first aspect, the present invention provides an anti-hair loss and oil control composition suitable for Asian men, the composition comprising the following ingredients in parts by weight: 0.5-10 parts of Melissa officinalis extract, 0.05-3 parts of Gorgon coral extract, 0.02-3 parts of Chamaecyparis obtusa leaf extract, and 0.05-0.8 parts of Black Willow bark extract.

[0009] Melissa officinalis extract can affect neuromodulators such as dopamine and cortisol to achieve emotional skin care. In addition, Melissa officinalis extract has strong antioxidant and free radical scavenging activity, which can effectively reduce oxidative stress damage in nerve cells, and may improve mood by reducing oxidative stress in nerve cells; the diterpenoid glycosides contained in Coralleaf extract have significant anti-inflammatory effects on the skin and can reduce redness of the skin; the cypress phenol in Cypress leaf extract can increase the autophagic activity of human hair papilla cells and mitochondrial autophagic activity, and promote the proliferation of hair papilla cells; the active ingredients in black willow bark extract can affect the secretion of sebaceous glands to achieve an oil control effect, and the phenylethanol glycosides can inhibit fat production. In addition, it can also inhibit the production of inflammatory factors and play a soothing function.

[0010] The present invention has found that when the above-mentioned plant extracts are compounded and used in specific dosages, the hair loss problem of sensitive skin in Asian men can be improved from multiple dimensions such as comprehensive mood regulation, activation of cell autophagy, oil control, and soothing. The anti-hair loss composition combines the anti-hair loss ingredients at the physiological level and the mood soothing ingredients at the psychological level, achieving a dual intervention in the hair loss problem, and all of them are plant ingredients, which is more suitable for use by Asian men with sensitive skin.

[0011] As a preferred embodiment of the first aspect, the mass ratio of the sum of the mass of the Melissa officinalis extract and the Gorgonian coral extract to the mass ratio of the sum of the mass of the Chamaecyparis obtusifolia extract and the black willow bark extract in the composition is: (Melissa officinalis extract + Gorgonian coral extract): (Chamaecyparis obtusifolia extract + black willow bark extract) = (1.1-8): (0.6-2.5). For example, the mass ratio of (Melissa officinalis extract + Gorgonian coral extract): (Chamaecyparis obtusifolia extract + black willow bark extract) can be, but is not limited to, 1.1:0.6, 1.5:1, 2:2, 4:1, 6:2, 8:2, 8:2.5.

[0012] As a preferred embodiment of the first aspect, the mass ratio of the sum of the mass of the Melissa officinalis extract and the Gorgonian coral extract to the sum of the mass of the Chamaecyparis obtusifolia leaf extract and the Willow of Black Bark extract in the composition is: (Melissa officinalis extract + Gorgonian coral extract): (Chamaecyparis obtusifolia leaf extract + Willow of Black Bark extract) = (2.3-6.7): (0.9-1.9).

[0013] As a preferred embodiment of the first aspect, the mass ratio of the sum of the mass of the Melissa officinalis extract and the Gorgonian coral extract to the sum of the mass of the Chamaecyparis obtusifolia extract and the Willow of Black Bark extract in the composition is: (Melissa officinalis extract + Gorgonian coral extract): (Chamaecyparis obtusifolia extract + Willow of Black Bark extract) = 4.5:1.3.

[0014] The present invention has found that the mass ratio of the mass sum of the lemon balm extract and the gorgonian extract to the mass sum of the cypress leaf extract and the black willow bark extract in the anti-hair loss and oil control composition will not only affect the TNF-α inhibition rate of the composition to a certain extent, thereby affecting the anti-inflammatory and oxidative stress protection of the composition; it also affects the improvement of the a* value and TEWL value of the composition, thereby affecting the soothing and skin barrier repair functions of the composition, and also affecting the anti-hair loss effect and oil control effect of the composition. When the mass sum of the lemon balm extract and the gorgonian extract and the mass sum of the cypress leaf extract and the black willow bark extract are further selected to be (1.1-8): (0.6-2.5), especially (2.3-6.7): (0.9-1.9), the obtained men's anti-hair loss composition has better oil control, anti-hair loss, anti-inflammatory, antipruritic, soothing and skin barrier repair functions; when the mass sum of the lemon balm extract and the gorgonian extract and the mass sum of the cypress leaf extract and the black willow bark extract The mass ratio is 4.5: 1.3, the overall comprehensive effect of the composition can be enhanced to the greatest extent.

[0015] As a preferred embodiment of the first aspect, in the composition, the mass ratio of the Melissa officinalis extract to the Gorgonian coral extract is Melissa officinalis extract: Gorgonian coral extract = (1-7): (0.1-1). For example, the mass ratio of the Melissa officinalis extract to the Gorgonian coral extract can be, but is not limited to, 1:0.1, 2:0.2, 4:0.5, 5:0.6, 6:0.8, 7:1.

[0016] As a preferred embodiment of the first aspect, in the composition, the mass ratio of the Melissa officinalis extract to the Gorgonian coral extract is Melissa officinalis extract:Gorgonian coral extract=(2-6):(0.3-0.7).

[0017] As a preferred embodiment of the first aspect, in the composition, the mass ratio of the Melissa officinalis extract to the Gorgonian coral extract is Melissa officinalis extract:Gorgonian coral extract=3.8:0.7.

[0018] The present invention has found that the mass ratio of the lemon balm extract and the gorgonian extract in the anti-hair loss and oil control composition will affect the TNF-α inhibitory ability and the a* value and TEWL value improvement effect of the composition to a certain extent, thereby affecting the anti-inflammatory and itching, redness improvement and reduction of the oxidative stress response of nerve cells, and the skin barrier repair and soothing effects of the composition. When the mass ratio of the lemon balm extract and the gorgonian extract is further selected to be (1-7): (0.1-1), especially (2-6): (0.3-0.7), the skin anti-inflammatory effect and nerve cell oxidative stress effect of the composition can be improved, and the mood-improving ability of the composition, as well as the skin barrier repair and soothing ability, can be enhanced. When the mass ratio of the lemon balm extract and the gorgonian extract is 3.8: 0.7, the overall comprehensive effect of the composition can be enhanced to the greatest extent.

[0019] As a preferred embodiment of the first aspect, in the composition, the mass ratio of the Chamaecyparis obtusifolia leaf extract to the Willow nigra bark extract is Chamaecyparis obtusifolia leaf extract: Willow nigra bark extract = (0.5-2): (0.1-0.5). For example, the mass ratio of the Chamaecyparis obtusifolia leaf extract to the Willow nigra bark extract is 0.5:0.1, 0.8:0.2, 1:0.2, 1.5:0.3, 2:0.5.

[0020] As a preferred embodiment of the first aspect, in the composition, the mass ratio of Chamaecyparis obtusifolia leaf extract to Salix nigra bark extract is Chamaecyparis obtusifolia leaf extract: Salix nigra bark extract = (0.7-1.5): (0.2-0.4).

[0021] As a preferred embodiment of the first aspect, in the composition, the mass ratio of Chamaecyparis obtusifolia leaf extract to Salix nigra bark extract is Chamaecyparis obtusifolia leaf extract: Salix nigra bark extract=0.9:0.4.

[0022] The present invention has found that the mass ratio of the cypress leaf extract to the black willow bark extract in the anti-hair loss and oil control composition will affect the composition's inhibition of TNF-α, a* value and TEWL value improvement effect to a certain extent, thereby affecting the composition's anti-inflammatory, soothing, skin barrier damage repair efficacy and the ability to increase hair papilla cells and reduce oil secretion. When the mass ratio of the cypress leaf extract to the black willow bark extract is further selected to be (0.5-2): (0.1-0.5), especially (0.7-1.5): (0.2-0.4), the composition's soothing and skin barrier damage repair ability can be improved, and the composition's anti-inflammatory, anti-hair loss and oil control effects can also be improved. When the mass ratio of the cypress leaf extract to the black willow bark extract is 0.9: 0.4, the overall comprehensive effect of the composition can be enhanced to the greatest extent.

[0023] As a preferred embodiment of the first aspect, the preparation method of the Melissa officinalis extract comprises the following steps: taking the flowers of Melissa officinalis, drying them, crushing them, adding them to a phosphate buffer mixture for enzymatic hydrolysis, collecting the enzymatic hydrolysis products, centrifuging them, filtering them, and the filtrate is the Melissa officinalis extract; wherein, the enzymatic hydrolysis uses cellulase.

[0024] As a preferred implementation of the first aspect, at least one of the following is satisfied:

[0025] (a) the mass ratio of the phosphate buffer to the lemon balm powder is (8-10):1; or / and the pH value of the phosphate buffer is 4.5-5;

[0026] (b) the mass ratio of the cellulase to the lemon balm powder is (1-1.5):100;

[0027] (c) the enzymolysis time is 10-30 min; or / and the enzymolysis temperature is 45-55°C.

[0028] The present invention finds that different extraction methods have an impact on the lemon balm extract, and affect the synergistic effect between the lemon balm extract and other components, affect the inhibition rate of the composition on TNF-α and the improvement effect of a* value and TEWL value, thereby affecting the anti-inflammatory and antipruritic effects of the composition, improving redness and reducing the oxidative stress response of nerve cells, and the repair and soothing effects on the skin barrier, thereby affecting the overall comprehensive effect of the composition.

[0029] In a second aspect, the present invention provides the use of the anti-hair loss composition for men as described in the first aspect in the preparation of an anti-hair loss product.

[0030] In a third aspect, the present invention provides a men's anti-hair loss product, the men's anti-hair loss product comprising the men's anti-hair loss composition according to the first aspect,

[0031] Compared with the prior art, the beneficial effects of the present invention are:

[0032] The present invention finds that lemon balm extract, gorgonian extract, cypress leaf extract and black willow bark extract have a synergistic effect from numerous plant extracts. The composition formed by specific compatibility can improve the hair loss problem of sensitive skin from multiple dimensions such as comprehensive mood regulation, activation of cell autophagy, oil control and soothing. The anti-hair loss composition combines the anti-hair loss ingredients at the physiological level and the mood soothing ingredients at the psychological level to achieve dual intervention in the hair loss problem, and all of them are plant ingredients, which is more suitable for use by Asian men with sensitive skin. DETAILED DESCRIPTION

[0033] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials. Further description of the raw materials used in the present invention:

[0034] The Chamaecyparis obtusifolia leaf extract was purchased from Xi'an Youbohui Biotechnology Co., Ltd., product name: Chamaecyparis obtusifolia leaf extract, item number: 111111;

[0035] Black willow bark extract was purchased from Xi'an Lishi Biotechnology Co., Ltd., product name: Black willow bark extract, item number: LS-PG;

[0036] Gorgonian coral extract was purchased from Lanzhou Waterless Biotechnology Co., Ltd., product name: Gorgonian coral extract;

[0037] Grape seed extract replaced the lemon balm extract, purchased from Shaanxi Yinuo Biotechnology Co., Ltd., product name: grape seed extract, item number: 5554;

[0038] Platycladus orientalis leaf extract replaced Chamaecyparis obtusa leaf extract, purchased from Shanxi Yirun Biotechnology Co., Ltd., product name: Platycladus orientalis leaf extract;

[0039] White willow bark extract replaced black willow bark extract, purchased from Xi'an Lishi Biotechnology Co., Ltd., product name: white willow bark extract, item number: LS-blp;

[0040] Hawthorn leaf extract replaced the coral extract and was purchased from Shaanxi Lvshengyuan Biological Products Manufacturing Co., Ltd., product name: Hawthorn leaf extract, item number: SXLSYSZYTQW;

[0041] The preparation method of lemon balm extract-1 is as follows:

[0042] Take commercially available lemon balm, dry and grind it, then pass it through a 40-mesh sieve, add 8 times the mass of phosphate buffer (PBS) and mix it, adjust the pH to 4.5, and add cellulase; stir and mix at 45°C for enzymatic hydrolysis, then place it at 90°C for 10 minutes to inactivate the enzyme, then centrifuge and filter it, and the filtrate is the lemon balm extract-1; wherein the mass ratio of cellulase to lemon balm powder is 1:100.

[0043] The preparation method of lemon balm extract-2 is as follows:

[0044] Take commercially available lemon balm, dry and crush it, then pass it through a 50-mesh sieve, add 9-mass phosphate buffer (PBS) and mix it, adjust the pH to 4.5, add cellulase, stir and mix at 50°C for enzymatic hydrolysis, then place it at 90°C for 20 minutes to inactivate the enzyme, then centrifuge and filter it, and the filtrate is the lemon balm extract-2; wherein the mass ratio of cellulase to lemon balm powder is 1.2:100.

[0045] The preparation method of lemon balm extract-3 is as follows:

[0046] Take commercially available lemon balm, dry and crush it, then pass it through a 60-mesh sieve, add 10 mass% phosphate buffer (PBS) and mix it, adjust the pH to 5.0, add cellulase, stir and mix at 50°C for enzymatic hydrolysis, then place it at 90°C for 30 minutes to inactivate the enzyme, then centrifuge and filter it, and the filtrate is the lemon balm extract-3; wherein the mass ratio of cellulase to lemon balm powder is 1.5:100.

[0047] The preparation method of lemon balm extract-4 is as follows:

[0048] Take commercially available lemon balm, dry and grind it, and then pass it through a 40-mesh sieve. Take 1g of lemon balm powder, add 20mL of 70% ethanol by volume, and perform ultrasonic extraction at 40°C for 30min. The power of ultrasonic extraction is 120W. The obtained product is centrifuged, and the supernatant is concentrated to 5mL to obtain lemon balm extract-4.

[0049] The preparation method of lemon balm extract-5 is as follows:

[0050] Take commercially available lemon balm, dry and grind it, and then pass it through a 40-mesh sieve. Take 1g of lemon balm powder, add 20mL of 70% ethanol by volume, and reflux extract at 80°C for 1h to obtain lemon balm extract-5.

[0051] The preparation method of the anti-hair loss and oil control composition for Asian men suitable for sensitive skin of the present invention is as follows: weigh the components in parts by weight as described in Table 1, add water to 20 g, stir and mix evenly, and perform ultrasound for 10 minutes to obtain the anti-hair loss and oil control composition.

[0052] Examples and Comparative Examples

[0053] The present application provides an anti-hair loss and oil control composition suitable for sensitive skin of Asian men. The components and weight portions of the composition are shown in Table 1, wherein the comparative example uses Example 1 as a reference, and the total amount of the missing components in Comparative Examples 3-6 is the same as that in Example 1.

[0054] Table 1:

[0055]

[0056]

[0057]

[0058] Effect example

[0059] This effect example uses cell experiments to prove the anti-inflammatory ability of the composition of the present invention, as follows:

[0060] Experimental methods:

[0061] Plating: RAW264.7 cells in the logarithmic growth phase were inoculated in 24-well plates at a cell density of 2×10 5 / mL, 0.5mL per well, mark the top cover of the cell plate after laying the plate, and place it in a carbon dioxide incubator for incubation for 24h;

[0062] Sample treatment: After incubating the cells for 24 hours, the supernatant was removed and 0.5 mL of the following samples were added respectively: ① blank control group: DMEM medium; ② LPS inflammation model group: LPS (1 μg / mL); ③ positive control group: 0.001% dexamethasone + LPS (1 μg / mL); ④ experimental group: 1% embodiment or comparative example composition + LPS (1 μg / mL);

[0063] Detection of inflammatory factors in cell supernatant: After the sample is treated with cells for 24 hours, the supernatant is aspirated into a centrifuge tube with a pipette, centrifuged at 1000r / min for 5 minutes, the precipitate is discarded and the supernatant is aspirated, and the change in the amount of inflammatory factors in the supernatant is detected using the Xinbosheng TNF-α inflammatory factor detection kit; the inhibition rate of TNF-α is calculated, TNF-α inhibition rate % = (TNF-α amount in the model group - TNF-α amount in the experimental group) / TNF-α amount in the model group * 100%; the higher the inhibition rate, the stronger the anti-inflammatory ability. The calculated results are shown in Table 2;

[0064] Table 2:

[0065]

[0066]

[0067] It can be seen from Table 1 that the anti-hair loss and oil control composition for men provided by the present invention can significantly improve the TNF-α inhibition rate, which indicates that the combination of the present invention has a strong anti-inflammatory effect.

[0068] It can be seen from Examples 1-14 and Comparative Examples 1-2 that the mass ratio of the components in the anti-hair loss and oil control composition will affect the ability of the composition to inhibit TNF-α, thereby affecting the overall anti-inflammatory effect of the composition.

[0069] It can be seen from Examples 1-7 that the mass ratio of the components in the composition affects the ability of the composition to inhibit TNF-α. When the mass ratio of the sum of the mass of the Melissa officinalis extract and the Gorgonian coral extract to the mass ratio of the sum of the mass of the Chamaecyparis obtusifolia extract and the black willow bark extract is limited to the range of (1.1-8):(0.6-2.5), the resulting composition has a strong anti-inflammatory effect, especially in the range of (2.3-6.7):(0.9-1.9), the resulting composition has a better anti-inflammatory effect, and when the mass ratio is 4.5:1.3 (Example 7), the effect is the best.

[0070] The present invention studies the influence of the mass ratio of the Melissa officinalis extract to the Gorgonian coral extract in the composition on the inhibition of TNF-α by the composition through Examples 8-10. It can be seen from Examples 8-10 that the mass ratio of the Melissa officinalis extract to the Gorgonian coral extract affects the effect of the composition on the inhibition of TNF-α, thereby affecting the anti-inflammatory efficacy of the composition. When the mass ratio of the Melissa officinalis extract to the Gorgonian coral extract is limited to the range of (1-7): (0.1-1), the ability of the composition to inhibit TNF-α can be improved, especially when it is in the range of (2-6): (0.3-0.7), the effect of the obtained composition is better. When the mass ratio is 3.8: 0.7 (Example 9), the effect is best.

[0071] The present invention studies the influence of the mass ratio of the cypress leaf extract and the black willow bark extract in the composition on the inhibition of TNF-α by the composition through Examples 11-13. It can be seen from Examples 11-13 that the mass ratio of the cypress leaf extract and the black willow bark extract affects the effect of the composition on the inhibition of TNF-α, thereby affecting the anti-inflammatory effect of the composition. When the mass ratio of the cypress leaf extract and the black willow bark extract is limited to the range of (0.5-2): (0.1-0.5), the ability of the composition to inhibit TNF-α can be improved, especially when the range is (0.7-1.5): (0.2-0.4), the effect of the obtained composition is better. When its mass ratio is 0.9: 0.4 (Example 12), its effect reaches the best.

[0072] In summary, the optimal ratio of each group in the composition of the present invention is: Melissa officinalis extract: Gorgonian coral extract: Chamaecyparis obtusifolia leaf extract: Willow nigra bark extract = 3.8:0.7:0.9:0.4 (Example 14).

[0073] The present invention studies the effect of different extraction methods of Melissa officinalis extracts on the ability of the composition to inhibit TNF-α through Examples 15-18. It can be seen from Examples 15-18 that the extraction method of Melissa officinalis extract has a certain effect on the effect of inhibiting TNF-α, thereby affecting the anti-inflammatory effect of the composition. When Melissa officinalis extract-2 is used, the anti-inflammatory effect of the composition obtained is the best (Example 15).

[0074] The TNF-α inhibition rate of Comparative Example 1-2 is 26%. This is because the mass ratios of the components in the composition of Comparative Example 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0075] The TNF-α inhibition rate of Comparative Examples 3-6 is 22-23%. This is because the lack of one of the components of the present invention in the composition of Comparative Examples 3-6 affects the synergistic effect between the components and reduces the efficacy of the composition.

[0076] The TNF-α inhibition rate of Comparative Examples 7-10 is 24-25%. This is because the composition of Comparative Examples 7-10 replaces one of the components in the combination group of the present invention with other plant extracts, which does not produce a synergistic effect with the specific components selected by the present invention, and also affects the synergistic effect between other components of the present invention, thereby reducing the efficacy of the composition.

[0077] Application Examples

[0078] The application examples of the present invention and the comparative application examples provide a men's anti-hair loss product, and the components (mass percentage) of the anti-hair loss product are shown in Table 3; wherein the anti-hair loss and oil control compositions used in application examples 1-18 are respectively the anti-hair loss and oil control compositions prepared in embodiments 1-18; application examples 19-20 use the composition prepared in embodiment 15; the anti-hair loss and oil control compositions used in comparative application examples 1-10 are respectively the anti-hair loss and oil control compositions prepared in comparative examples 1-10; in addition, a blank application example is set, and the only difference between the blank application example and application example 1 is that no anti-hair loss and oil control composition is added;

[0079] Table 3:

[0080]

[0081] This embodiment provides a method for preparing shampoo, and the preparation method comprises the following steps:

[0082] (1) Add polyquaternium-10 to deionized water and stir to disperse, and then add other materials of phase A after uniform dispersion, heat to 85° C., stir to dissolve uniformly, and then keep warm for standby use to obtain prefabricated phase A;

[0083] (2) Add the ingredients of phase B at 80-85°C, stir thoroughly to dissolve evenly until transparent, and keep warm to remove foam;

[0084] (3) Cool down to below 45°C, add the raw materials of phase C one by one, and stir evenly;

[0085] (4) Continue to add the raw materials of phase D and mix well to obtain the men's anti-hair loss shampoo.

[0086] Test Example 1 Test of TEWL and a* value improvement of anti-hair loss products

[0087] A number of male volunteers aged 18-45 were selected. On the day of the visit, the subjects sat in a constant temperature and humidity room with a temperature of 21±1℃ and a humidity of 50±10% for 30 minutes. The skin color probe Skin-Colorimeter CL-400 and the transepidermal water loss probe were used. TM Hex (Courage+Khazaka) tests the initial a* value and initial TEWL value at the hairline. The subjects were randomly divided into 31 groups, 5 people in each group, and the anti-hair loss products provided by the present invention were randomly used. Each index was tested at 8 weeks and 12 weeks, and the improvement of the TEWL value and a* value of each volunteer's skin at 8 weeks and 12 weeks was analyzed. The improvement was represented by the improvement rate of each value, and the calculation method was as follows:

[0088] Improvement rate relative to the initial value = |(X 使用后 -X 使用前 ) / X 使用前 ×100%|; TEWL value indicates the skin barrier repair effect, and the greater the improvement rate, the stronger the skin barrier repair ability; a* value indicates the soothing function, and the greater the improvement rate, the stronger the soothing ability. The calculated data are shown in Table 4;

[0089] Table 4:

[0090]

[0091]

[0092] It can be seen from Table 4 that the shampoo provided by the present invention has excellent effects of soothing and repairing the skin barrier. Specifically, at the 12th week, the a* improvement rate % was above 11%, and the TEWL value improvement rate % was above 13%.

[0093] It can be seen from Application Examples 1-14 and Comparative Examples 1-2 that the mass ratio of the components in the men's anti-hair loss and oil control composition will affect the ability of the composition to improve TEWL value and a* value, thereby affecting the overall soothing and skin barrier repairing effect of the composition.

[0094] It can be seen from Application Examples 1-7 that the mass ratio of the components in the composition will affect the composition's ability to soothe and repair the skin barrier. When the mass ratio of the sum of the mass of the Melissa officinalis extract and the Coralleaf extract to the mass ratio of the sum of the mass of the Chamaecyparis obtusifolia extract and the Willow bark extract is limited to the range of (1.1-8):(0.6-2.5), the resulting composition has a strong soothing and repairing effect on the skin barrier, especially in the range of (2.3-6.7):(0.9-1.9), the resulting composition has a better effect of soothing and repairing the skin barrier, and when the mass ratio is 4.5:1.3 (Application Example 7), the effect is the best.

[0095] The present invention studies the influence of the mass ratio of the melissa extract to the gorgonian extract in the composition on the improvement of the TEWL value of the composition through application examples 8-10. It can be seen from application examples 8-10 that the mass ratio of the melissa extract to the gorgonian extract affects the effect of the composition on improving the TEWL value, thereby affecting the barrier repair efficacy of the composition. When the mass ratio of the melissa extract to the gorgonian extract is limited to the range of (1-7):(0.1-1), the ability of the composition to improve the TEWL value can be improved, especially when the mass ratio is (2-6):(0.3-0.7), the effect of the obtained composition is better. When the mass ratio is 3.8:0.7 (application example 9), the effect is optimal.

[0096] The present invention studies the influence of the mass ratio of the cypress leaf extract and the black willow bark extract in the composition on the improvement of the a* value of the composition through application examples 11-13. It can be seen from application examples 11-13 that the mass ratio of the cypress leaf extract and the black willow bark extract affects the effect of the composition to improve the a* value, thereby affecting the soothing effect of the composition. When the mass ratio of the cypress leaf extract and the black willow bark extract is limited to the range of (0.5-2): (0.1-0.5), the soothing ability of the composition can be improved, especially when (0.7-1.5): (0.2-0.4), the effect of the obtained composition is better. When its mass ratio is 0.9: 0.4 (application 12), its effect reaches the best.

[0097] In summary, the best combination of the present invention obtained from the above results is: the mass ratio of Melissa officinalis extract, Gorgon coral extract, Chamaecyparis obtusifolia leaf extract and Willow nigra bark extract is 3.8:0.7:0.9:0.4 (Application Example 14).

[0098] The present invention studies the effects of lemon balm extracts obtained by different extraction methods on the soothing and barrier repairing abilities of the composition through Application Examples 15-18. It can be seen that the extraction method of the lemon balm extract has an effect on the TEWL value improvement effect, thereby affecting the barrier repairing effect. When the lemon balm extract-2 is used, the obtained composition has the best effect (Application Example 15).

[0099] The TEWL value improvement rate of Comparative Application Example 1-2 is 12%, and the a* value improvement is 10%. This is because the mass ratios of the components in the compositions of Comparative Application Example 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0100] The TEWL value improvement rate of comparative application example 3-6 is 11%, and the a* value improvement is 9%. This is because the lack of one of the components of the present invention in the composition of comparative application example 3-6 affects the synergistic effect between the components and reduces the efficacy of the composition.

[0101] The TEWL value improvement rate of comparative application examples 7-10 is 11-12%, and the a* value improvement is 9-10%. This is because the comparative application examples 7-10 compositions replace one of the components in the combination group of the present invention with other plant extracts, which do not produce a synergistic effect with the specific components selected by the present invention, and also affect the synergistic effect between other components of the present invention, thereby reducing the efficacy of the composition.

[0102] Test Example 2: Test on the anti-hair loss effect and emotional impact of anti-hair loss products

[0103] A number of male volunteers aged 18-45 years old, all with different degrees of hair loss problems, were selected and divided into 31 groups with 5 people in each group. The subjects used the anti-hair loss products of the above application examples or comparative application examples once a day. After 8 and 12 weeks of use, the hair loss was counted. The 60-times combing method was used to count the number of hair lost in 60 combings. The number of hair loss was counted before using the product and after 8 and 12 weeks of use. The subjects did not wash their hair within 48±4 hours before each visit and did not comb their hair on the day of the visit. The hair loss reduction rate was calculated, hair loss reduction rate / %=(number of hair loss roots at 0 weeks-number of hair loss roots at 8 / 12 weeks) / number of hair loss roots at 0 weeks; the calculated data are shown in Table 5:

[0104] In addition, volunteers filled out the Self-Assessment Manikin (SAM) to evaluate the current skin feeling. The evaluation was based on a 9-point system, with higher scores representing higher levels of pleasure. The subjects scored before and after the first use, and the results were expressed as improvement rate, improvement rate / % = (score after use - score after use) / score before use. The evaluation results are shown in Table 5.

[0105] Table 5

[0106]

[0107]

[0108] It can be seen from Table 5 that the shampoo provided by the present invention has excellent anti-hair loss and mood-improving effects. Specifically, at the 12th week, the obtained hair loss reduction rate was above 40%.

[0109] It can be seen from Application Examples 1-14 and Comparative Examples 1-2 that the mass ratio of the components in the anti-hair loss composition for men will affect the anti-hair loss effect of the composition.

[0110] It can be seen from Application Examples 1-7 that the mass ratio of the components in the composition will affect the anti-hair loss effect of the composition. When the mass ratio of the sum of the mass of the Melissa officinalis extract and the Coralleaf extract to the mass ratio of the sum of the mass of the Chamaecyparis obtusifolia extract and the Willow bark extract is limited to the range of (1.1-8):(0.6-2.5), the obtained composition has a strong anti-hair loss effect, especially when (2.3-6.7):(0.9-1.9), the obtained composition has a better anti-hair loss effect, and when the mass ratio is 4.5:1.3 (Application Example 7), the effect is the best.

[0111] The present invention studies the influence of the mass ratio of the Melissa officinalis extract to the Gorgonian coral extract in the composition on the anti-hair loss effect of the composition through application examples 8-10. It can be seen from application examples 8-10 that the mass ratio of the Melissa officinalis extract to the Gorgonian coral extract affects the anti-hair loss effect of the composition. When the mass ratio of the Melissa officinalis extract to the Gorgonian coral extract is limited to the range of (1-7):(0.1-1), the anti-hair loss effect of the composition can be improved, especially when the mass ratio is (2-6):(0.3-0.7), the effect of the obtained composition is better. When the mass ratio is 3.8:0.7 (application example 9), the effect is best.

[0112] The present invention studies the influence of the mass ratio of the cypress leaf extract and the black willow bark extract in the composition on the anti-hair loss effect of the composition through application examples 11-13. It can be seen from application examples 11-13 that the mass ratio of the cypress leaf extract and the black willow bark extract affects the anti-hair loss effect of the composition. When the mass ratio of the cypress leaf extract and the black willow bark extract is limited to the range of (0.5-2): (0.1-0.5), the ability of the composition to improve the a* value can be improved, especially when (0.7-1.5): (0.2-0.4), the effect of the obtained composition is better. When its mass ratio is 0.9: 0.4 (application 12), its effect reaches the best.

[0113] In summary, the best combination of the present invention obtained from the above results is: the mass ratio of Melissa officinalis extract, Gorgon coral extract, Chamaecyparis obtusifolia leaf extract and Willow nigra bark extract is 3.8:0.7:0.9:0.4 (Application Example 14).

[0114] The present invention studies the effect of different extraction methods of Melissa officinalis extract on the anti-hair loss effect of the composition through application examples 15-18. It can be seen that the extraction method of Melissa officinalis extract has a certain effect on the anti-hair loss effect. When Melissa officinalis extract-2 is used, the obtained composition has the best effect (application example 15).

[0115] The hair loss reduction rate of comparative application examples 1-2 is 35-36%. This is because the mass ratios of the components in the compositions of comparative application examples 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the compositions.

[0116] The hair loss reduction rate of comparative application examples 3-6 is 27-30%. This is because the lack of one of the components of the present invention in the composition of comparative application examples 3-6 affects the synergistic effect between the components and reduces the efficacy of the composition.

[0117] The hair loss reduction rate of comparative application examples 7-10 is 32-34%. This is because the comparative application examples 7-10 compositions replace one of the components in the combination group of the present invention with other plant extracts, which do not produce a synergistic effect with the specific components selected by the present invention, and also affect the synergistic effect between other components of the present invention, thereby reducing the efficacy of the composition.

[0118] Test Example 3 Consumer Use Test of the Anti-hair Loss Product of the Present Invention Suitable for Asian Men with Sensitive Skin

[0119] This test example verifies the applicability and soothing effect of the product prepared in Application Example 1 on Asian men with sensitive skin, and specifically includes the following steps:

[0120] 30 male subjects with sensitive facial skin and recent sensitive skin symptoms were selected to participate in the test. This product was distributed to them. Each subject applied an appropriate amount of the product to the corresponding part every day for a 28-day sensitive skin suitability test. Consumers completed a questionnaire survey on the improvement of indicators such as anti-hair loss effect, soothing effect, oil control effect and irritation. See Table 6 for details:

[0121] Table 6:

[0122]

[0123]

[0124] It can be seen from Table 6 that the anti-hair loss product of the present invention with the anti-hair loss and oil control composition added has the effects of anti-hair loss, soothing, and relieving itching, and can allow the subjects to experience a refreshing and comfortable feeling. At the same time, the anti-hair loss product of the present invention is mild and non-irritating, and can be used by people with sensitive skin.

[0125] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An anti-hair loss and oil control composition suitable for Asian men, characterized in that: The composition comprises the following ingredients in parts by weight: 0.5-10 parts of melissa officinalis extract, 0.05-3 parts of gorgonian coral extract, 0.02-3 parts of cypress leaf extract, and 0.05-0.8 parts of black willow bark extract.

2. The anti-hair loss and oil control composition according to claim 1, characterized in that The mass ratio of the sum of the mass of the Melissa officinalis extract and the Gorgonian coral extract to the sum of the mass of the Chamaecyparis obtusifolia leaf extract and the Willow of Black Bark extract in the composition is: (Melissa officinalis extract + Gorgonian coral extract): (Chamaecyparis obtusifolia leaf extract + Willow of Black Bark extract) = (1.1-8): (0.6-2.5).

3. The anti-hair loss and oil control composition according to claim 2, characterized in that: The mass ratio of the sum of the mass of the Melissa officinalis extract and the Gorgonian coral extract to the sum of the mass of the Chamaecyparis obtusifolia extract and the Willow of Black Bark extract in the composition is: (Melissa officinalis extract + Gorgonian coral extract): (Chamaecyparis obtusifolia extract + Willow of Black Bark extract) = (2.3-6.7): (0.9-1.9).

4. The anti-hair loss and oil control composition according to claim 1, characterized in that: In the composition, the mass ratio of the lemon balm extract to the gorgonian coral extract is: lemon balm extract:gorgonian coral extract = (1-7): (0.1-1).

5. The anti-hair loss and oil control composition according to claim 4, characterized in that: In the composition, the mass ratio of the lemon balm extract to the gorgonian extract is: lemon balm extract:gorgonian extract = (2-6): (0.3-0.7).

6. The anti-hair loss and oil control composition according to claim 1, characterized in that: In the composition, the mass ratio of Chamaecyparis obtusifolia leaf extract to Willow nigra bark extract is: Chamaecyparis obtusifolia leaf extract: Willow nigra bark extract = (0.5-2): (0.1-0.5).

7. The anti-hair loss and oil control composition according to claim 6, characterized in that: In the composition, the mass ratio of Chamaecyparis obtusifolia leaf extract to Willow nigra bark extract is: Chamaecyparis obtusifolia leaf extract: Willow nigra bark extract = (0.7-1.5): (0.2-0.4).

8. The anti-hair loss and oil control composition according to any one of claims 1 to 7, characterized in that: The preparation method of the lemon balm extract comprises the following steps: The flowers of Melissa officinalis are dried, crushed and added to a phosphate buffer mixture for enzymatic hydrolysis, and the enzymatic hydrolysis products are collected, centrifuged and filtered, and the filtrate is the Melissa officinalis extract; wherein the enzymatic hydrolysis uses cellulase.

9. The anti-hair loss and oil control composition according to claim 8, characterized in that: Satisfy at least one of the following: (a) the mass ratio of the phosphate buffer to the lemon balm powder is (8-10):1; (b) the pH value of the phosphate buffer is 4.5-5; (b) the mass ratio of the cellulase to the lemon balm powder is (1-1.5):100; (c) The enzymolysis time is 10-30 min; the enzymolysis temperature is 45-55°C.

10. Use of the anti-hair loss and oil control composition according to any one of claims 1 to 9 in the preparation of an anti-hair loss product.

11. A men's anti-hair loss product, characterized in that: The men's anti-hair loss product comprises the anti-hair loss and oil control composition according to any one of claims 1 to 9.

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