Oily composition for nourishing and conditioning skin and application thereof

Abstract

The invention discloses an oily composition for nourishing and conditioning skin and application thereof, the composition is a product obtained by co-fermentation of a raw material and yeast, and the raw material of the composition is a fermentation raw material combination 1 or a fermentation raw material combination 2; wherein the fermentation raw material combination 1 comprises perigord truffle, white truffle and tricholoma matsutake; the fermentation raw material combination 2 comprises radix pseudostellariae, codonopsis pilosula and ginseng. According to the composition disclosed by the invention, through compounding and synergistic effects of all the components, skin aging resistance is realized, free radicals can be effectively removed, and the composition is mild and safe, has soothing and repairing effects on people with sensitive skin, has relatively good hydrophilicity and is easy to absorb.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and in particular relates to an oily composition for nourishing and conditioning the skin and an application thereof. Background Art

[0002] Conventional cosmetics follow the principles of cosmetic formula design. On the basis of functional raw materials, thickeners, emulsifiers, preservatives and fragrances are added to maintain the stability of their appearance and meet the needs of commercialization. In addition, antioxidant cosmetics also add a variety of antioxidant ingredients to improve their antioxidant effects, including antioxidant extracts from plant sources and some non-plant-derived antioxidants. For pregnant women, lactating women and other people with sensitive skin, this increases the burden on the skin and even increases the risk of irritation and sensitivity.

[0003] As the market demand for natural and all-plant ingredients in cosmetics increases, more and more cosmetic formulas have reduced the use of non-plant-derived raw materials. However, existing antioxidant cosmetic technologies and inventions are only compounding and post-processing of plant cosmetic raw materials, without controlling and changing the source of raw materials and production technology to meet the market consumers' demands for all-plant ingredients, natural and mild safety. Summary of the invention

[0004] The object of the present invention is to overcome at least one of the deficiencies of the prior art and to provide an oily composition for nourishing and conditioning the skin and its application.

[0005] The technical solution adopted by the present invention is:

[0006] In the first aspect, the present invention provides an oily composition for nourishing and conditioning the skin, wherein the composition is a product obtained by co-fermentation of raw materials and yeast, and the raw materials of the composition are fermentation raw material combination 1 or fermentation raw material combination 2; wherein, fermentation raw material combination 1 contains black truffle, white truffle and matsutake; and fermentation raw material combination 2 contains Pseudostellaria heterophylla, Codonopsis pilosula and ginseng.

[0007] In some examples, the mass ratio of black truffle: white truffle: matsutake in the fermentation raw material combination 1 is 1: (8-12): (26-32).

[0008] In some examples, the mass ratio of Pseudostellaria heterophylla: Codonopsis pilosula: Ginseng ginseng in the fermentation raw material combination 2 is 5: (7-9): (9-11).

[0009] In some examples, the mass composition of the components in the fermentation raw material combination 1 is: 0.5% black truffle, 5% white truffle, 15% matsutake, 0.5% natural vitamin E oil, and the remainder is coconut oil caprylate / caprate.

[0010] In some examples, the mass composition of the components in the fermentation raw material combination 2 is: 5% ginseng, 8% codonopsis, 10% pseudoginseng, 1% natural vitamin E oil, and the remainder is coconut oil caprylate / caprate.

[0011] In some examples, the preparation method of the oily composition is as follows:

[0012] 1) Select raw materials, crush and screen;

[0013] 2) Extract, filter and concentrate the product using supercritical carbon dioxide extraction method to obtain an extract;

[0014] 3) The extract obtained in step 2) is used as a raw material to prepare a culture medium for fermentation, and the mixture of the small molecule active metabolites and yeast fermentation is a composition for nourishing and conditioning the skin.

[0015] In some examples, the culture medium is composed of: 5-30wt% extract, 0.2-1.0wt% potassium dihydrogen phosphate, 0.5-3wt% yeast powder, 0.2-1.0wt% sodium chloride, 5-20wt% sucrose, and the balance is water.

[0016] In some examples, the effective ingredient is the oily composition for nourishing and conditioning the skin according to claim 1.

[0017] In some examples, the skin care product further comprises a moisturizer, a thickener, an emollient, a pH adjuster, a penetration enhancer, an antioxidant, and a preservative.

[0018] In some examples, the mass addition amount of the oily composition for nourishing and conditioning the skin is 0.1-10%.

[0019] The beneficial effects of the present invention are:

[0020] 1) The composition of the present invention achieves anti-aging of the skin through the synergistic effect of the various components, and can effectively remove free radicals. The composition is mild and safe, has a soothing and repairing effect on people with sensitive skin, has good hydrophilicity, and is easy to absorb;

[0021] 2) The composition of the present invention can effectively relieve sensitive skin from external stimulation, does not contain hormones, antihistamines, etc., thereby avoiding the stimulation and sensitization of the skin by chemical agents. DETAILED DESCRIPTION

[0022] The following disclosure provides many different embodiments or examples for implementing different solutions of the present invention.

[0023] The specific compositions of the active ingredients of the oily composition for nourishing and conditioning the skin in different examples in terms of mass percentage are shown in Tables 1 and 2. In Examples 1-3 and Comparative Examples 1-3, 0.5% natural vitamin E oil is added, and the balance is coconut oil alcohol-caprylate / caprate; in Examples 4-6 and Comparative Examples 4-6, 1% natural vitamin E oil is added, and the balance is coconut oil alcohol-caprylate / caprate:

[0024] Table 1

[0025] Group Black Truffle / % White Truffle / % Matsutake / % Example 1 0.45 5 15 Example 2 0.55 5 15 Example 3 0.5 5 15 Comparative Example 1 0 5 15 Comparative Example 2 0.5 0 15 Comparative Example 3 0.5 5 0

[0026] Table 2

[0027] Group Pseudostellariae / % Codonopsis pilosula / % Ginseng / % Example 4 4.5 8 10 Example 5 5.5 8 10 Example 6 5 8 10 Comparative Example 4 0 8 10 Comparative Example 5 5 0 10 Comparative Example 6 5 8 0

[0028] The preparation method of the essence of each example is as follows:

[0029] 1) According to the mass ratio in Table 1 or Table 2, select the raw materials and grind and sieve them separately;

[0030] 2) Extraction by supercritical carbon dioxide extraction, filtration, concentration, and obtaining an extract;

[0031] 3) using the extract obtained in step 2) as a raw material to prepare a culture medium for solid-state fermentation in a constant temperature incubator at 28-30° C. for 3-5 days, wherein the culture medium comprises: 20wt% extract, 0.6wt% potassium dihydrogen phosphate, 2wt% yeast powder, 0.6wt% sodium chloride, 15wt% sucrose, and the remainder is water; and mixing the obtained small molecule active metabolites and a mixture of yeast fermentation.

[0032] 4) The skin nourishing and conditioning composition is mixed with a moisturizer, a thickener, an emollient, a pH adjuster, a penetration enhancer, an antioxidant, and a preservative at 35-45° C. to obtain the essence corresponding to each example.

[0033] Efficacy verification

[0034] 1. Efficacy test

[0035] 1. Test equipment:

[0036] 1. The present invention uses the Finnish Delfin VapoMeter SWL5201 transepidermal water loss measuring instrument. VapoMeter uses the closed chamber measurement principle, so the measurement is not affected by the indoor airflow. The measurement time is short, ensuring that the blocking of normal evaporation is minimized.

[0037] 2. Take photos using Delfin Antera 3D from Finland. Antera3D uses innovative optical methods and complex mathematical algorithms to obtain three-dimensional images. This allows data to be extracted from images related to the three-dimensional shape of the skin, which will be able to quantify the effects of treatment and monitor changes over time. For example, measure the texture, smoothness, and depth and width of fine lines and wrinkles of the skin. With its multispectral analysis, Antera software can also measure pigmentation and redness, which will provide meaningful information about sun damage, veins, etc.

[0038] 2. Experimental Principle:

[0039] TEWL value, transepidermal water loss (TEWL) measures the change in water vapor pressure near the skin surface. TEWL value indicates the loss of water from the stratum corneum and is an important criterion for evaluating the barrier function of the stratum corneum. The lower the TEWL value of the skin, the better the barrier function of the skin, and vice versa. By testing the TEWL value of skin water loss, the efficacy of moisturizing and repairing cosmetics can be evaluated. It can also be used in allergic patch tests, contact dermatitis, physical therapy, burns and monitoring of new tissue to promptly detect whether the protective function of the skin has been damaged. The unit of TEWL is: g / hm 3 .

[0040] 3. Test population:

[0041] There are 6 groups in total, with 28 people in each group, including volunteers aged 18-60 who meet the experimental requirements.

[0042] 4. Test conditions:

[0043] At room temperature of 20±2℃ and relative humidity of 50±10%, real-time dynamic monitoring was carried out and the subjects sat quietly for 30 minutes before the relevant data were tested.

[0044] 5. Experimental process:

[0045] The experiment was divided into 6 groups. The first group of testers applied Example 1 on the left face and Comparative Example 1 on the right face; the second group of testers applied Example 2 on the left face and Comparative Example 2 on the right face; and so on, until the sixth group applied Example 6 on the left face and Comparative Example 6 on the right face;

[0046] Apply it on the face twice a day, morning and evening, for 4 weeks, record the TEWL value, then take a photo of each volunteer's left and right cheeks with Antera 3D, record the average RED value of the skin redness, and archive it.

[0047] 6. Experimental results:

[0048] Transepidermal water loss (TEWL), skin redness average (RED) test:

[0049] In this test, the TEWL measured by the subject in week 0 is set as TEWL0, and the TEWL in week 4 is set as TEWL4. The TEWL change value is △TEWL, △TEWL = TEWL4- TEWL0, △TEWL is a negative number, and the lower the value, the less water loss of the skin after four weeks, that is, the better the skin barrier function is repaired, and the healthier the skin is;

[0050] The RED of the 0th week is set as RED0, and the RED of the 4th week is set as RED4. The RED change value is △RED, △RED = RED4-RED0, △RED is a negative number, and the lower the value, the less redness of the skin, that is, the less inflammatory reaction of the basal skin, the more comfortable the skin, and the better the soothing and anti-allergic effect of the product. Table 3 shows the average △TEWL value and △RED value of each group of 25 people, as follows:

[0051] Table 3

[0052]

[0053] Experimental results:

[0054] From the experimental results in Table 3, we can see that:

[0055] 1. At the addition ratios of Example 3 and Example 6, the △TEWL value and △RED value are relatively small, that is, the less the skin moisture loss, the better the integrity of the skin barrier, the better the ability to resist environmental damage, the less likely it is to cause allergies, and the less redness of the skin, which means that the inflammatory response at the base of the skin is smaller and the soothing effect on sensitive skin is better.

[0056] 2. The △TEWL and △RED values ​​of any example are smaller than those of the comparative example, indicating that removing any component in the composition of the present invention will increase the corresponding △TEWL and △RED values, that is, removing any component will weaken the function of the composition of the present invention in soothing sensitive skin and strengthening the skin barrier.

[0057] DPPH free radical scavenging rate test

[0058] DPPH free radical scavenging rate can characterize the efficiency of scavenging free radicals, thereby judging the antioxidant effect of cosmetics and further reflecting its whitening effect. The testing principle is as follows:

[0059] 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable nitrogen-centered organic free radical. The DPPH method was proposed in 1958 and is widely used to quantitatively determine the antioxidant capacity of biological samples, phenolic substances and foods. This method is based on the characteristics that the DPPH free radical has a single electron, has a strong absorption at 517nm, and its alcohol solution is purple. When a free radical scavenger is present, its absorption gradually disappears due to its pairing with a single electron, and its fading degree is quantitatively related to the number of electrons it accepts. Therefore, a spectrophotometer can be used for rapid quantitative analysis to detect the free radical scavenging situation, thereby evaluating the antioxidant capacity of the sample. Its capacity is expressed by the scavenging rate. The greater the scavenging rate, the better its antioxidant effect. Therefore, the smaller the probability of stimulating the formation of melanin, the better the whitening effect.

[0060] Reagents: 0.0178mmol / L DPPH-anhydrous methanol solution is added to the cuvette, and then 3ml of the test solution is added. After mixing, the absorbance value is immediately measured at a wavelength of 517nm, and the absorbance value is recorded as A1. Then, the absorbance value is measured after being stored at 37℃ in the dark for 30min, and it is recorded as A2. The blank control is to add only DPPH-anhydrous methanol solution, and its absorbance value is recorded as A0. Calculate the DPPH free radical scavenging rate according to the following formula:

[0061] DPPH free radical scavenging rate (%) = [1-(A1-A2) / A0] × 100%, take the average value of three measurements, and the test results are shown in Table 4.

[0062] Table 4

[0063] Group DPPH free radical scavenging rate (%) Example 1 48.54 Example 2 55.76 Example 3 64.45 Example 4 47.80 Example 5 52.69 Example 6 61.33 Comparative Example 1 25.57 Comparative Example 2 18.54 Comparative Example 3 32.41 Comparative Example 4 31.24 Comparative Example 5 28.45 Comparative Example 6 18.64

[0064] It can be seen from the data in Table 4 that the efficiency of scavenging free radicals using the composition of the present invention in the example is better than that in the comparative example, and the antioxidant effect of the composition of the present invention is very excellent. The composition of the present invention can effectively scavenges free radicals generated by the body itself or free radicals generated by ultraviolet radiation in vitro, thereby greatly reducing the content of melanin produced in the skin, that is, achieving a better skin whitening effect.

[0065] The above is a further detailed description of the present invention, which should not be regarded as a limitation on the specific implementation of the present invention. For ordinary technicians in the technical field to which the present invention belongs, simple deduction or replacement without departing from the concept of the present invention is within the protection scope of the present invention.

Claims

1. An oily composition for nourishing and conditioning the skin, characterized in that: The oily composition is a product obtained by co-fermentation of raw materials and yeast, and the raw materials of the composition are fermentation raw material combination 1 or fermentation raw material combination 2; wherein, the fermentation raw material combination 1 contains black truffle, white truffle and matsutake; the fermentation raw material combination 2 contains Pseudostellaria heterophylla, Codonopsis pilosula and ginseng.

2. The oily composition according to claim 1, characterized in that In the fermentation raw material combination 1, the mass ratio of black truffle: white truffle: matsutake is 1: (8-12): (26-32).

3. The oily composition according to claim 1, characterized in that In the fermentation raw material combination 2, the mass ratio of Pseudostellaria heterophylla: Codonopsis pilosula: Ginseng is 5: (7-9): (9-11).

4. The oily composition according to claim 1 or 2, characterized in that The mass composition of the components in the fermentation raw material combination 1 is: 0.5% black truffle, 5% white truffle, 15% matsutake, 0.5% natural vitamin E oil, and the remainder is coconut oil alcohol-caprylate / caprate.

5. The oily composition according to claim 1 or 3, characterized in that The mass composition of the components in the fermentation raw material combination 2 is: 5% ginseng, 8% codonopsis, 10% pseudoginseng, 1% natural vitamin E oil, and the remainder is coconut oil alcohol-caprylate / caprate.

6. The oily composition according to claim 1, wherein the preparation method of the oily composition is as follows: 1) Select raw materials, crush and screen; 2) Extract, filter and concentrate the product using supercritical carbon dioxide extraction method to obtain an extract; 3) The extract obtained in step 2) is used as a raw material to prepare a culture medium for fermentation, and the mixture of the small molecule active metabolites obtained by mixing and yeast fermentation is an oily composition for nourishing and conditioning the skin.

7. The oily composition according to claim 6, characterized in that The culture medium comprises: 5-30wt% extract, 0.2-1.0wt% potassium dihydrogen phosphate, 0.5-3wt% yeast powder, 0.2-1.0wt% sodium chloride, 5-20wt% sucrose, and the balance is water.

8. A skin care product for nourishing and conditioning the skin, comprising auxiliary materials and functional ingredients, characterized in that: The effective ingredient is the oily composition for nourishing and conditioning the skin according to claim 1.

9. The skin care product according to claim 8, characterized in that: The skin care product also includes a moisturizer, a thickener, an emollient, a pH adjuster, a penetration enhancer, an antioxidant, and a preservative.

10. The skin care product according to claim 8, characterized in that: The mass addition amount of the oily composition for nourishing and conditioning the skin is 0.1 to 10%.

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