A composition for preventing hair loss and strengthening hair, and its preparation method and application

Abstract

The present invention discloses a composition for preventing hair loss and strengthening hair, as well as its preparation method and application, specifically belonging to the field of cosmetic preparations. The composition comprises a composite fermentation product filtrate and a sericin concentrate. The preparation method comprises the following steps: mixing mulberry leaves, safflower, arborvitae leaves, agarwood, and prunella vulgaris, crushing and sieving to obtain a composite powder; extracting the composite powder with an ethanol solution to obtain a crude extract; acclimating and culturing Leuconostoc citriodora with the crude extract to obtain an acclimated composite bacterial solution; fermenting and culturing the acclimated composite bacterial solution, centrifuging the supernatant, filtering and sterilizing the solution, and obtaining a composite fermentation product filtrate. The composition provides a good hair loss prevention and strengthening effect, with an improvement rate of hair follicle density exceeding 70%.

Description

Technical Field

[0001] The invention belongs to the field of cosmetic preparations, and particularly relates to a hair loss prevention and hair strengthening composition, a preparation method and an application thereof. Background Art

[0002] Hair loss, a common physiological phenomenon, has complex and diverse mechanisms. It can generally be divided into two categories: physiological and non-physiological. Hair growth follows a natural cyclical process, encompassing the growth phase, the regression phase, and the resting phase. In a healthy state, after sufficient growth during the growth phase, hair naturally enters the regression and resting phases, resulting in a certain amount of physiological shedding. During this process, the replacement of old and new hair maintains a dynamic balance and ensures relative stability of hair volume. Typically, individuals naturally shed between 50 and 100 hairs per day, which is considered a normal physiological phenomenon.

[0003] However, when the amount of hair loss exceeds this range, especially when the average daily hair loss exceeds 100 strands, the balance between hair growth and shedding is disrupted, leading to gradual thinning of hair and even baldness. This abnormal, excessive hair loss not only seriously affects personal appearance but can also place a heavy psychological burden and mental stress on patients. This type of hair loss, which significantly affects appearance, such as thinning hair and receding hairlines caused by various non-physiological factors, is classified as non-physiological hair loss, also known as pathological hair loss.

[0004] Pathological hair loss can be divided into five categories according to its main causes: androgenic alopecia, immunodeficiency alopecia, traumatic alopecia, nutritional alopecia and drug-induced alopecia. As the problem of pathological hair loss becomes increasingly serious, many anti-hair loss products have emerged on the market to solve this problem. These products are mainly divided into two camps: one is based on the theory of traditional Chinese medicine, using a variety of herbal compound extracts to try to slow down hair loss through nourishing or oil control mechanisms, but consumer feedback shows that most products have limited effects; the other is the integration of Western medicine ingredients, such as sildenafil, minoxidil, etc. as active substances. Although they can significantly inhibit hair loss and promote hair growth in the early stage, the side effects associated with long-term use (such as sexual dysfunction, abnormally low blood pressure, etc.) cannot be ignored, which limits the feasibility of its long-term application.

[0005] Therefore, given the shortcomings of existing anti-hair loss products in terms of effectiveness and safety, there is an urgent need to develop a new anti-hair loss technology or product that can effectively prevent hair loss and has good safety, so as to meet market demand and improve the quality of life of hair loss patients. Summary of the Invention

[0006] In view of the defects of the existing anti-hair loss and hair strengthening products in the prior art that are not effective or have significant side effects, the purpose of the present invention is to provide an anti-hair loss and hair strengthening composition. The composition promotes hair follicle cell proliferation and hair growth by regulating the growth metabolism of hair follicle cells, inhibiting the release of androgens and inflammatory factors, and regulating the scalp microecology, thereby exerting the effect of preventing hair loss and strengthening hair, and is safe for the human body and has no side effects.

[0007] To achieve the above objectives, the present invention discloses the following technical solutions:

[0008] In a first aspect, the present invention provides a composition for preventing hair loss and strengthening hair, comprising the following components in parts by mass:

[0009] 15-18 parts of composite fermentation product filtrate;

[0010] 1 part of sericin concentrate;

[0011] The preparation method of the composite fermentation product filtrate comprises the following steps:

[0012] (1) Mix mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal, crush them, and sieve them to obtain a composite powder;

[0013] (2) extracting the composite powder with an ethanol aqueous solution and removing the ethanol to obtain a crude extract;

[0014] (3) using the crude extract to acclimate and culture Leuconostoc citrifolia to obtain an acclimatized composite bacterial solution;

[0015] (4) The acclimated composite bacterial liquid is inoculated into the fermentation medium containing the crude extract for fermentation culture, and then the supernatant is centrifuged and filtered for sterilization to obtain the composite fermentation product filtrate.

[0016] Preferably, in step (1), the mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal are mixed in a mass ratio of (4.5-5.5):(0.09-0.11):(6-8):(1.8-2.2):(1.8-2.2).

[0017] Preferably, in step (2), the ethanol aqueous solution is a 50% v / v ethanol aqueous solution.

[0018] Preferably, the step (2) is to extract the composite powder with a 50% v / v ethanol aqueous solution at room temperature for 15 days, remove the ethanol after filtration, and obtain a crude extract.

[0019] Preferably, the step (3) is to inoculate Leuconostoc citriodora into an acclimation culture medium containing a crude extract, and then culture the culture medium in a shaker at a speed of 150-200 r / min and a temperature of 25-30° C. for 1-2 days to obtain an acclimated composite bacterial solution;

[0020] The step (4) is to dilute the acclimated composite bacterial solution with sterile water to a viable bacterial count of 1×10 7 -1×10 9 CFU / mL of diluted bacterial solution, and then inoculated the diluted bacterial solution into the fermentation medium at an inoculum rate of 9-12% v / v. Fermentation was carried out for 48-60 hours at a temperature of 25-28°C and a stirring speed of 150-200 r / min. The supernatant was then centrifuged and filtered for sterilization to obtain a composite fermentation product filtrate.

[0021] Further preferably, the acclimation medium comprises the following components: 10 wt% crude extract, carbon source, nitrogen source, vitamins, inorganic salts, deionized water, and a pH of 6.5±0.2;

[0022] The fermentation medium comprises the following components: 10 wt% of a crude extract, a carbon source, a nitrogen source, vitamins, inorganic salts, and deionized water, and has a pH of 6.5±0.2.

[0023] In a second aspect, the present invention provides a use of the anti-hair loss and hair strengthening composition described in the first aspect in the preparation of a hair care product having the efficacy of preventing hair loss and hair strengthening.

[0024] In a third aspect, the present invention provides an anti-hair loss and hair strengthening essence, comprising the anti-hair loss and hair strengthening composition of the first aspect;

[0025] The amount of the anti-hair loss and hair strengthening composition added to the anti-hair loss and hair strengthening essence is 10-50 wt %.

[0026] Preferably, the invention further comprises a fibroin concentrate, a moisturizing agent, a preservative, a thickener, an emulsifier, a pH regulator and a solvent.

[0027] In a fourth aspect, the present invention provides a method for preparing the anti-hair loss and hair strengthening essence according to the third aspect, comprising the following steps:

[0028] The silk fibroin concentrate, the anti-hair loss and hair strengthening composition, moisturizer, thickener, emulsifier, preservative and solvent are mixed and stirred evenly, and then a pH regulator is added to adjust the pH to 6.5-7 to obtain the anti-hair loss and hair strengthening essence.

[0029] In the present invention:

[0030] Mulberry leaves can activate luciferase, prevent the occurrence of atopic dermatitis, have strong antioxidant properties, accelerate the biosynthesis of hyaluronic acid in the cortex, reduce the irritation of fruit acid, inhibit and regulate the activity of 5a-reductase, and promote hair growth; Prunella vulgaris can stimulate hair growth, activate enzyme tyrosinase, and have anti-inflammatory, antibacterial and analgesic effects. It also has an inhibitory effect on 5a-reductase, prevents and treats hair loss caused by high androgen levels, activates tyrosinase activity, increases the production of melanin, inhibits methioninase, reduces the production of methyl mercaptan and reduces body odor; Agarwood has the function of inhibiting hyaluronic acid. The decomposition of hyaluronic acid enhances the moisturizing effect of the skin and promotes hair growth. The resin components in agarwood also have the function of deep cleansing and absorbing dirt and oil, which can keep the skin fresh and clean; Platycladus orientalis leaves have the effect of cooling blood and stopping bleeding, resolving phlegm and relieving cough, and can also grow black hair. It has a certain auxiliary therapeutic effect on blood-heat hair loss and premature graying of hair, and has the function of controlling oil and cleaning the scalp. Platycladus orientalis leaves can stimulate scalp blood circulation and play an antibacterial and anti-inflammatory role; safflower can be used to improve microcirculation, promote scalp blood circulation, thereby helping hair growth and reducing hair loss.

[0031] The present invention found that lemon-colored Leuconostoc can enrich the effective ingredients in the crude extracts of mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal. At the same time, the fermentation of Leuconostoc itself can produce active substances such as bacteriocins, organic acids, and extracellular polysaccharides, which have the effects of regulating scalp barrier function damage, hormone metabolism disorders, activating the physiological functions of hair follicles and hair bulbs, and promoting scalp blood circulation; in addition, it is also compounded with sericin, which has the effects of strong moisturizing and precise oil control, and can effectively regulate the oil-water balance of the scalp, improve the scalp barrier function, and provide a good material basis for healthy hair growth.

[0032] Beneficial effects of the present invention:

[0033] 1. The anti-hair loss and hair strengthening composition provided by the present invention has the effect of promoting the proliferation of hair papilla cells and thus promoting hair growth;

[0034] 2. The anti-hair loss and hair-fixing composition provided by the present invention, when applied to the scalp, can increase the production of cytokines related to hair growth, resist the production of inhibitory factors, and have the effect of promoting and fixing hair growth;

[0035] 3. The anti-hair loss and hair-fixing composition provided by the present invention containing the above composition has a good hair-fixing and anti-hair loss effect, and the hair follicle density improvement rate reaches more than 70%. DETAILED DESCRIPTION

[0036] To make the objectives, technical solutions, and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to specific embodiments. However, the following embodiments are merely preferred embodiments of the present invention and are not exhaustive. For the sake of clarity, not all features of the actual embodiments are described.

[0037] Based on the embodiments in the implementation manner, other embodiments obtained by those skilled in the art without making any creative work shall all fall within the protection scope of the present invention.

[0038] In the present invention

[0039] Mulberry leaves: Morus alba L. of the Moraceae family were purchased from the Qingping Chinese Herbal Medicine Market in Guangzhou;

[0040] Safflower: Carthamus tinctorius L., Asteraceae, purchased from Guangzhou Qingping Chinese Herbal Medicine Market;

[0041] Platycladus orientalis (L.) Franco, Cupressaceae, purchased from Guangzhou Qingping Chinese Herbal Medicine Market;

[0042] Aquilaria sinensis (Lour.) Spreng., Thymelaeaceae, purchased from Guangzhou Qingping Chinese Herbal Medicine Market;

[0043] Prunella vulgaris: Prunella vulgaris L., Lamiaceae, purchased from Guangzhou Qingping Chinese Herbal Medicine Market;

[0044] Leuconostoc citreum: scientific name Leuconostoc citreum, strain number GDMCC NO.1.919, purchased from Guangdong Provincial Microbiological Culture Collection Center;

[0045] Sericin concentrate: a sericin concentrate prepared according to the preparation method of Example 1 in Chinese patent CN116083510B;

[0046] Yeast extract: purchased from Beijing Hongrun Baoshun Technology Co., Ltd.

[0047] Silk fibroin concentrate: purchased from Suzhou Soho Biomaterials Technology Co., Ltd.

[0048] Other raw materials are commercially available.

[0049] Preparation of anti-hair loss and hair solidifying composition

[0050] Step (1), taking dried mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal in a mass ratio of (4.5-5.5):(0.09-0.11):(6-8):(1.8-2.2):(1.8-2.2), mixing, and grinding through a 60-mesh sieve to obtain a composite powder;

[0051] Step (2), extracting the composite powder with a 50% v / v ethanol aqueous solution at room temperature for 15 days, removing the ethanol after filtration, and obtaining a crude extract;

[0052] Step (3), preparation of acclimation medium: crude extract 10wt%, glucose 2.5wt%, peptone 0.1wt%, beef extract 0.2wt%, KH2PO4 0.1wt%, MgSO4 0.15wt%, vitamin B1 0.01wt%, balance deionized water, pH = 6.5 ± 0.2;

[0053] Step (4), acclimatization culture: scrape 0.5cm 2 The activated Leuconostoc citrifolia slant strain is inoculated into the prepared acclimation medium and cultured in a shaker at a speed of 150-200 r / min and a temperature of 25-30°C for 1-2 days to obtain an acclimated composite bacterial solution;

[0054] Step (5), preparation of fermentation medium: crude extract 10wt%, glucose 1.5wt%, peptone 0.1wt%, yeast extract 0.5wt%, KH2PO4 0.1wt%, MgSO4 0.15wt%, vitamin B1 0.01wt%, the balance is deionized water, pH = 6.5 ± 0.2;

[0055] Step (6): dilute the acclimated composite bacterial solution with sterile water to a viable bacterial count of 1×10 7 -1×10 9 CFU / mL of diluted bacterial solution, and then inoculating the diluted bacterial solution into the fermentation medium at an inoculum rate of 9-12% v / v, fermenting and culturing for 48-60 hours at a temperature of 25-28°C and a stirring speed of 150-200 r / min, then centrifuging and taking the supernatant, filtering and sterilizing through a 0.22 μm polyethersulfone filter element to obtain a composite fermentation product filtrate;

[0056] Step (7), taking the composite fermentation product filtrate obtained in step (6) and the sericin protein concentrate in a mass ratio of (15-18):1 and mixing them to obtain an anti-hair loss and hair solidification composition.

[0057] Table 1 Preparation parameters of Examples and Comparative Examples

[0058]

[0059] Table 1 Preparation parameters of Examples and Comparative Examples (continued)

[0060]

[0061] Note: “-” in the table means not added.

[0062] Comparative Example 1:

[0063] The raw materials lack mulberry leaves, and the remaining steps are consistent with Example 2.

[0064] Comparative Example 2:

[0065] The raw materials lack safflower, and the remaining steps are consistent with Example 2.

[0066] Comparative Example 3:

[0067] The raw materials lacked Platycladus orientalis leaves, and the remaining steps were consistent with those in Example 2.

[0068] Comparative Example 4:

[0069] There is no agarwood in the raw materials, and the remaining steps are consistent with Example 2.

[0070] Comparative Example 5:

[0071] The raw materials lacked Prunella vulgaris, and the remaining steps were consistent with those in Example 2.

[0072] Comparative Example 6:

[0073] Without performing steps (3) to (6), the crude extract and the sericin concentrate are directly mixed to obtain an anti-hair loss and hair solidifying composition. The remaining steps are the same as those in Example 2.

[0074] Comparative Example 7:

[0075] The mass ratio of mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal was adjusted to 1:1:1:1:1, and the remaining steps were consistent with Example 2.

[0076] Performance Testing

[0077] Dermal papilla cell proliferation promotion experiment

[0078] Cell culture: Human dermal papilla cells (HFDPCs) were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% (v / v) fetal bovine serum and 1% (v / v) antibiotics at 37°C and 5% CO2.

[0079] Sample preparation: Examples 1-3 and Comparative Examples 1-7 were diluted with DMEM culture medium to prepare test samples with a concentration of 10 wt%.

[0080] CCK-8 assay: Dermal papilla cells were plated at 5×10 4Cells were seeded at a density of 100 μg / well in a 96-well plate and incubated for 24 hours. 10 wt% of the test sample was added to each well. Serum-free DMEM medium and serum-supplemented DMEM medium were used as blank and negative controls, respectively. Three replicates were run in each group, and the results were averaged. After 24 hours of treatment, 10% CCK-8 solution was added to each well and incubated for an additional hour. The absorbance (OD) was measured at a detection wavelength of 450 nm.

[0081] The results were calculated as follows: cell viability (%) = (OD sample - OD blank) / (OD negative control - OD blank) × 100%. The results are shown in Table 2.

[0082] Table 2 Relative cell viability (%)

[0083]

[0084]

[0085] Result analysis:

[0086] When co-incubated with the anti-hair loss and hair solidifying composition, the cell viability was significantly improved compared with the negative control group without the anti-hair loss and hair solidifying composition, reaching a maximum of 156.22%, proving that the anti-hair loss and hair solidifying composition can promote the proliferation of hair papilla cells, thereby promoting hair growth.

[0087] Experimental study on the regulation of cytokines related to hair growth in dermal papilla cells

[0088] Cell culture: Human dermal papilla cells (HFDPCs) were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% (v / v) fetal bovine serum and 1% (v / v) antibiotics at 37°C and 5% CO2.

[0089] Sample preparation: Examples 1-3 and Comparative Examples 1-7 were diluted with DMEM medium to a concentration of 10 wt% of the test sample, minoxidil was diluted with DMEM medium to a concentration of 0.0002 wt% as a positive control group, and DMEM medium without any ingredients was added as a blank control group. Three parallel treatments were performed in each group, and the results were averaged.

[0090] Experimental method: The regulatory effects of growth factors VEGF and TGF-β1 related to hair growth in the culture supernatant obtained after treatment with the test sample were assessed using an ELISA kit. The operation was performed according to the kit instructions. The results are shown in Table 3.

[0091] Table 3 VEGF and TGF-β1 content

[0092]

[0093]

[0094] Result analysis:

[0095] Co-incubation with the anti-hair loss and hair-fixing composition increased the production of VEGF in human dermal papilla cells. VEGF plays a role in hair follicle growth and is a hallmark indicator of hair growth promotion. Furthermore, minoxidil, a positive control, significantly increased VEGF production in human dermal papilla cells compared to the blank control. Furthermore, both the positive control and the anti-hair loss and hair-fixing composition significantly reduced the production of TGF-β1, a factor produced by androgen through reductase conversion and binding to androgen receptors. This factor regulates the apoptosis of dermal papilla cells, leading to hair follicle atrophy and ultimately hair loss. These results indicate that the anti-hair loss and hair-fixing composition, when applied to the scalp, can increase the production of cytokines associated with hair growth and counteract the production of inhibitory factors, thereby promoting both hair growth and hair growth.

[0096] Application Example Preparation

[0097] Deionized water, hydroxyethyl acrylate / methoxyethyl acrylate copolymer, 1,3-propylene glycol, butylene glycol, isopentyl glycol and ethylhexylglycerin are added to a reactor and mixed and stirred evenly. Then, carbohydrate isomers, silk fibroin concentrate, capryloyl glycine, arginine, anti-hair loss and hair strengthening composition or composite fermentation product filtrate or sericin concentrate are added to the reactor in sequence and mixed and stirred evenly. Lactic acid is added to adjust the pH to 6.8 to prepare an anti-hair loss and hair strengthening essence.

[0098] Table 4 Raw materials and their mass percentages in the anti-hair loss and hair strengthening essence

[0099]

[0100] Note: “-” in the table means not added.

[0101] Evaluation of anti-hair loss effects

[0102] Experimental plan:

[0103] Fifty volunteers with hair loss, aged 30 to 55 years, were randomly divided into five groups of 10 each. Each group was assigned to use Application Examples 1-5, applying 2 mL of sample solution to the scalp in the affected area once in the morning and evening, and massaging for 5-10 minutes. This was continued for six months. No other cosmetics, medications, or health supplements that could affect the results were used during the trial.

[0104] The hair follicle density changes of the subjects' hair loss areas (area 4 cm × 4 cm) were detected using a hair follicle analyzer before and after use, and the average value was taken. The results are shown in Table 5.

[0105] At the same time, the subjects evaluated the improvement of hair loss symptoms before and after use. The results are shown in Table 6.

[0106] Table 5 Evaluation results of hair follicle density changes (number / cm 2 )

[0107] Group Before use After use Improvement rate / % Application Example 1 45.7 78.2 71.12％ Application Example 2 44.9 71.1 58.35％ Application Example 3 47.3 69.6 47.15％ Application Example 4 46.1 57.3 24.30％ Application Example 5 44.7 53.7 20.13％

[0108] Table 6 Evaluation results of changes in hair loss (based on the number of evaluators)

[0109] Group No change Slight improvement Significant improvement Stop hair loss Application Example 1 0 0 7 3 Application Example 2 0 4 5 1 Application Example 3 0 8 2 0 Application Example 4 3 6 1 0 Application Example 5 6 4 0 0

[0110] Result analysis:

[0111] According to the effect verification of Application Examples 1-3, it can be seen that as the concentration of the anti-hair loss and hair solidifying composition increases, the anti-hair loss and hair solidifying effect becomes better, showing a certain concentration dependence, which proves that the anti-hair loss and hair solidifying composition provided by the present invention has a good hair loss prevention effect;

[0112] The anti-hair loss effect of Application Example 2 is better than that of Application Examples 4 and 5. The results show that the anti-hair loss and hair solidifying composition of the present invention is better than using the composite fermentation product filtrate alone or using the sericin protein concentrate alone in improving hair loss symptoms. The anti-hair loss and hair solidifying composition of the present invention has excellent anti-hair loss efficacy, and the composite fermentation product filtrate and the sericin protein concentrate have a certain synergistic effect.

[0113] Although specific embodiments of the present invention have been described above, those skilled in the art will appreciate that these are merely illustrative and that the scope of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, and such changes and modifications are intended to fall within the scope of the present invention.

Claims

1. A composition for preventing hair loss and strengthening hair, characterized in that: Calculated by mass, it includes the following components: 15-18 parts of composite fermentation product filtrate; 1 part of sericin concentrate; The preparation method of the composite fermentation product filtrate comprises the following steps: (1) Mix mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal, crush them, and sieve them to obtain a composite powder; (2) extracting the composite powder with an ethanol aqueous solution, removing the ethanol, and obtaining a crude extract; (3) using the crude extract to acclimate and culture Leuconostoc citrifolia to obtain an acclimatized composite bacterial solution; (4) inoculating the acclimated composite bacterial solution into a fermentation medium containing the crude extract for fermentation culture, then centrifuging the supernatant, filtering and sterilizing to obtain a composite fermentation product filtrate; In the step (1), mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal are mixed according to a mass ratio of (4.5-5.5):(0.09-0.11):(6-8):(1.8-2.2):(1.8-2.2); The sericin concentrate is prepared by the preparation method of Example 1 in Chinese patent CN116083510B.

2. The anti-hair loss and hair solidifying composition according to claim 1, characterized in that In step (2), the ethanol aqueous solution is a 50% v / v ethanol aqueous solution.

3. The anti-hair loss and hair solidifying composition according to claim 1, characterized in that: The step (2) is to extract the composite powder at room temperature for 15 days using a 50% v / v ethanol aqueous solution, and remove the ethanol after filtering to obtain a crude extract.

4. The anti-hair loss and hair solidifying composition according to claim 1, characterized in that: The step (3) is to inoculate Leuconostoc citriodora into an acclimation culture medium containing a crude extract, and then culture the culture medium in a shaker at a speed of 150-200 r / min and a temperature of 25-30° C. for 1-2 days to obtain an acclimated composite bacterial solution; Step (4) is to dilute the acclimated composite bacterial solution with sterile water to a viable bacterial count of 1×10 7 -1×10 9 CFU / mL of diluted bacterial solution, and then inoculated the diluted bacterial solution into the fermentation medium at an inoculum rate of 9-12% v / v. Fermentation was carried out for 48-60 hours at a temperature of 25-28°C and a stirring speed of 150-200 r / min. The supernatant was then centrifuged and filtered for sterilization to obtain a composite fermentation product filtrate.

5. The anti-hair loss and hair solidifying composition according to claim 4, characterized in that: The acclimation medium comprises the following components: 10 wt% crude extract, carbon source, nitrogen source, vitamins, inorganic salts, deionized water, and a pH of 6.5±0.2; The fermentation medium comprises the following components: 10 wt% of a crude extract, a carbon source, a nitrogen source, vitamins, inorganic salts, and deionized water, and has a pH of 6.5±0.

2.

6. Use of the anti-hair loss and hair strengthening composition according to any one of claims 1 to 5 in the preparation of a hair care product having the efficacy of preventing hair loss and strengthening hair.

7. An anti-hair loss and strengthening essence, characterized in that: A composition for preventing and solidifying hair loss comprising any one of claims 1 to 5; The amount of the anti-hair loss and hair strengthening composition added to the anti-hair loss and hair strengthening essence is 10-50 wt %.

8. The anti-hair loss and strengthening essence according to claim 7, characterized in that: The invention also contains fibroin concentrate, moisturizing agent, preservative, thickener, emulsifier, pH regulator and solvent.

9. The method for preparing the anti-hair loss and strengthening essence according to claim 8, characterized in that: The following steps are involved: The silk fibroin concentrate, the anti-hair loss and hair strengthening composition, moisturizer, thickener, emulsifier, preservative and solvent are mixed and stirred evenly, and then a pH regulator is added to adjust the pH to 6.5-7 to obtain the anti-hair loss and hair strengthening essence.

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