An oil-control and soothing composition, its use, and a washing and care product

Abstract

The present invention belongs to the technical field of hair care, and discloses an oil-control and soothing composition, uses thereof, and a washing and care product. The oil-control and soothing composition includes semen vaccariae extract, flaxseed extract, fructuskochiae extract, and tropaeolum majus extract. The semen vaccariae extract, flaxseed extract, fructuskochiae extract, and tropaeolum majus extract are obtained by compound extraction of semen vaccariae, flaxseed, fructuskochiae, and tropaeolum majus. The weight ratio of semen vaccariae, flaxseed, fructuskochiae, and tropaeolum majus is 1-3:1-5:0.5-2:0.1-1.5. Through the compounding of the semen vaccariae extract, flaxseed extract, fructuskochiae extract, and tropaeolum majus extract, the present invention synergistically improves the oil-control effect of the composition from aspects such as improving scalp metabolism, regulating scalp microcirculation, and promoting hair follicle nutrient absorption, and can produce a synergistic effect of inhibiting 5α-reductase.

Description

Technical Field

[0001] The present invention relates to the technical field of hair care, and particularly relates to an oil-control and soothing composition, its use, and a hair washing and care product. Background Art

[0002] In modern society, due to factors such as eating habits and life pressure, hair loss among young people has gradually become a common phenomenon. Hair loss is mainly caused by follicle blockage and hair root corrosion due to excessive sebum secretion on the scalp. Normally, sebum has a protective effect on the scalp and hair, keeping the scalp moist and making the hair shiny and smooth. However, if stimulated by factors such as irregular work and rest, staying up late, excessive mental stress, and often eating a diet rich in oil and spicy food, the sebaceous glands will secrete excessive sebum. Excessive oil on the hair will cause pore blockage, affecting the respiration of hair follicles, and thus leading to hair loss.

[0003] Therefore, oil control is not only for keeping the scalp and hair clean and healthy, but also for preventing possible scalp problems and hair loss.

[0004] The secretion of scalp sebum is closely related to 5α-reductase. 5α-reductase is a protease, and its abnormal activity or increase will stimulate the conversion of testosterone in the body into dihydrotestosterone (DHT). The increase in 5α-reductase will convert male hormones into dihydrotestosterone, which binds to androgen receptors in the sebaceous glands, causing the sebaceous glands to fall into a state of excessive secretion, thus resulting in excessive scalp sebum secretion. In addition, high levels of dihydrotestosterone will also affect the cell function of hair follicles, leading to a shortened hair follicle growth cycle, smaller hair follicles, and hair follicle atrophy, and ultimately may cause problems such as hair loss.

[0005] Therefore, the inhibition of 5α-reductase is a key research and development direction for hair washing and care products.

[0006] Such as the prior art 1: Chinese Patent Application No. 202411354989.6 discloses a method and application for fermenting Platycladus orientalis leaves with a composite bacterium. The composite bacterium includes Lactococcus lactis and Pichia pastoris. The method includes the following steps: (1) pretreatment of Platycladus orientalis leaves; (2) enzymatic treatment of Platycladus orientalis leaves; (3) fermentation of Platycladus orientalis leaves; (4) treatment of the fermentation broth. The description of this patent application states: "Using the optimized fermentation method, more flavonoids can be obtained, better anti-hair loss effects can be achieved, and it is more conducive to the application of Platycladus orientalis leaves"; and the description of this patent application also verifies the effect of the fermentation product of Platycladus orientalis leaves in inhibiting 5α-reductase through experiments.

[0007] Similarly, Prior Art 2: Chinese Patent Application No. 2024113149408 discloses the application of plant extracts in the preparation of oil-control and anti-hair loss products. The plant extracts contain ganoderic triterpenoids. Its specification states that "active ingredients in many plants, such as triterpenoids, flavonoids, alkaloids, etc., have various biological activities such as antioxidant, anti-inflammatory, and antibacterial, which contribute to scalp health and thus improve the hair loss condition to a certain extent". This patent application uses the ganoderic triterpenoids obtained by extraction to inhibit 5α-reductase and thus applies it to anti-hair loss washing and care products.

[0008] Prior Art 2 shows that the active ingredients of many plants all have the effect of inhibiting 5α-reductase and improving the hair loss state; at the same time, Prior Art 1 also verifies the authenticity of this theory. However, in washing and care products, the content of a single component needs to be restricted to ensure safety in use. Therefore, using only a single component for anti-hair loss has a weak effect, and Prior Art 1 and Prior Art 2 do not deeply study how to improve the inhibitory effect on 5α-reductase by combining different active ingredients of multiple plants. Summary of the Invention

[0009] One of the purposes of the present invention is to provide an oil-control and soothing composition, avoiding the problem that washing and care products rely solely on a single component and thus have a weak inhibitory effect on 5α-reductase, and effectively improving the inhibitory effect of the composition on 5α-reductase through the combination of multiple components.

[0010] Another purpose of the present invention is to provide a preparation method of an oil-control and soothing composition, extracting the active ingredients of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus through composite extraction to achieve an excellent inhibitory effect on 5α-reductase.

[0011] At the same time, the present invention also provides the use of the oil-control and soothing composition. This oil-control and soothing composition is used to prepare washing and care products, and washing and care products with excellent oil-control and soothing improvement conditions can be obtained.

[0012] Furthermore, the present invention also provides a washing and care product. This shampoo contains a certain amount of the oil-control and soothing composition, and effectively improves the oil-control and soothing efficacy of the washing and care product through the compounding of multiple components under the limitation of the dosage of a single component.

[0013] To achieve the above purposes, the present invention provides an oil-control and soothing composition, including Vaccaria segetalis extract, Linum usitatissimum extract, Kochia scoparia extract, and Tropaeolum majus flower extract. The Vaccaria segetalis extract, Linum usitatissimum extract, Kochia scoparia extract, and Tropaeolum majus flower extract are obtained by composite extraction of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus. The weight ratio of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus is 1 - 3:1 - 5:0.5 - 2:0.1 - 1.5.

[0014] Fenugreek extract is rich in a variety of active ingredients. When these ingredients act on the scalp, they can effectively reduce the production of excessive oil and keep the scalp refreshed. At the same time, it can also refine pores, improve the scalp environment, and promote nutrient absorption by hair follicles, making the scalp look healthier and more delicate.

[0015] Flaxseed extract is rich in unsaturated fatty acids. When acting on the scalp, these ingredients can improve the metabolism of the scalp and make the stratum corneum of the scalp more resilient, thus helping to control the secretion of oil. At the same time, unsaturated fatty acids also have a certain antioxidant effect, which is beneficial to hair growth and can assist in the treatment of hair loss caused by seborrheic dermatitis. In addition, essential fatty acids such as Omega-3 and Omega-6 in flaxseed extract can also promote scalp microcirculation and provide sufficient nutrition for hair follicles. This nutrient delivery helps hair growth, making hair thicker and more lustrous, which also helps to control oil to a certain extent.

[0016] The saponin component rich in Kochia scoparia seed extract has a good cleansing effect and can remove dirt and oil on the scalp. At the same time, its antioxidant and anti-inflammatory effects also help to improve the scalp environment and thus control oil secretion.

[0017] Although nasturtium extract does not have a clear oil-control effect, it has the effect of nourishing hair and enhancing hair elasticity; and after being combined with fenugreek extract, linseed extract, and Kochia scoparia extract, the oil-control effect of the composition can be significantly improved.

[0018] The present invention, through the compounding of fenugreek extract, linseed extract, Kochia scoparia extract and nasturtium extract, synergistically enhances the oil control effect of the composition from the directions of improving scalp metabolism, regulating scalp microcirculation, and promoting nutrient absorption of hair follicles, and can produce a synergistic inhibition of 5α-reductase.

[0019] In addition, the combination of fenugreek extract, flaxseed extract, Kochia scoparia extract and nasturtium extract synergistically enhances the soothing effect of the composition. ‌‌

[0020] The composite extraction of the fenugreek extract, linseed extract, Kochia scoparia extract and nasturtium extract comprises the following steps:

[0021] Step 1: Add fenugreek, linseed, Kochia scoparia and nasturtium to water, stir at 60-70° C. for 3-4 hours to obtain a mixture, and centrifuge the mixture to obtain an extract.

[0022] Step 2: Concentrate the extract to 20-30% of the original mass at 65±5°C and -0.06~-0.1MPa to obtain a concentrated solution;

[0023] Step 3: Add an equal mass of 95% ethanol to the concentrated solution for alcohol precipitation treatment, and perform solid-liquid separation to obtain the alcohol precipitation solution;

[0024] Step 4: Transfer the alcohol precipitation solution to a falling film concentrator, and under the concentration temperature of 60±5°C and the concentration pressure of -0.06~-0.1MPa, vacuum concentrate the filtrate until the ethanol content is zero to obtain a non-alcohol concentrated solution;

[0025] Step 5: Add butanediol and pure water to the non-alcohol concentrated solution for ultrafiltration to obtain an ultrafiltrate. The addition amount of butanediol is 1-15 times the mass of the non-alcohol concentrated solution, and the addition amount of pure water is 2-20 times the mass of the non-alcohol concentrated solution.

[0026] Preferably, in Step 5, the pore size of the ultrafiltration membrane for ultrafiltration is 0.05-0.45um, the ultrafiltration pressure is 0.1-0.3MPa, and the ultrafiltration temperature is 30±10°C.

[0027] Further, it further includes Step 6: Add a preservative to the ultrafiltrate and sterilize.

[0028] The preservative is at least one of p-hydroxyacetophenone, hexylene glycol, octanohydroxamic acid, ethylhexylglycerin, pentylene glycol, sodium benzoate, phenoxyethanol.

[0029] The present invention also provides the use of an oil-control and soothing composition in preparing a washing and care product.

[0030] Preferably, the washing and care product includes shampoo, hair cream, hair conditioner, essence, lotion, cream, hair care essential oil, dry hair spray.

[0031] Further, the present invention also provides a washing and care product containing 0.1-30wt% of the oil-control and soothing composition.

[0032] Compared with the prior art, the beneficial effects of the present invention are:

[0033] (1) Through the compounding of the extracts of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus, the present invention synergistically improves the oil-control effect of the composition from aspects such as improving scalp metabolism, regulating scalp microcirculation, and promoting follicle nutrient absorption, and can produce a synergistic effect of inhibiting 5α-reductase;

[0034] (2) The combination of the extracts of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus in the present invention produces a synergistic effect of enhancing the soothing effect of the composition. Description of the Drawings

[0035] Figure 1 is a chicken embryo diagram before using the oil-control and soothing composition in Example 6 of the present invention;

[0036] Figure 2 It is a chicken embryo image after using the oil-control and soothing composition of Example 6 of the present invention. Detailed implementation manners

[0037] The present invention will be further described below in conjunction with embodiments, but it does not constitute any limitation to the present invention. Any limited modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0038] In order to elaborate on the technical content of the present invention, further explanations will be given below in conjunction with the implementation manners.

[0039] In the following examples and comparative examples, the properties of Vaccaria segetalis, flaxseed, Kochia scoparia, Tropaeolum majus, Hamamelis mollis, Olea europaea leaf, Sophora flavescens, and Rosmarinus officinalis are all powders after being crushed and passed through a 100-mesh sieve.

[0040] Unless otherwise specified, the parts mentioned in the following examples and comparative examples are all parts by weight.

[0041] In the following examples and comparative examples, the compositions are all obtained by composite extraction through the following steps:

[0042] Step 1: Add Vaccaria segetalis or Hamamelis mollis, flaxseed or Olea europaea leaf, Kochia scoparia or Sophora flavescens, Tropaeolum majus or Rosmarinus officinalis to water, stir at a temperature of 65 ± 2 °C for 3 - 4 h to obtain a mixture, and centrifuge the mixture to obtain an extract.

[0043] Step 2: Concentrate the extract at 65 ± 5 °C and -0.08 MPa to 20% of the original mass to obtain a concentrated solution;

[0044] Step 3: Add an equal mass of 95% ethanol to the concentrated solution for alcohol precipitation treatment, and perform solid-liquid separation to obtain an alcohol precipitation solution;

[0045] Step 4: Transfer the alcohol precipitation solution to a falling film concentrator, and vacuum concentrate the filtrate at a concentration temperature of 60 ± 5 °C and a concentration pressure of -0.08 MPa until the ethanol content is zero to obtain a non-alcohol concentrated solution;

[0046] Step 5: Add 1.5 times the mass of the non-alcohol concentrated solution of butanediol and 2.5 times the mass of the non-alcohol concentrated solution of pure water to the non-alcohol concentrated solution, and then perform ultrafiltration using a ultrafiltration membrane with a pore size of 0.25 um at a ultrafiltration pressure of 0.2 MPa and a ultrafiltration temperature of 30 ± 10 °C to obtain an ultrafiltrate.

[0047] Example 1

[0048] An oil-control and soothing composition is obtained by composite extraction using 3 parts of Vaccaria segetalis, 2 parts of flaxseed, 0.5 part of Kochia scoparia, and 0.2 part of Tropaeolum majus.

[0049] Example 2

[0050] An oil-control and soothing composition is obtained by compound extraction with 2 parts of Vaccaria segetalis, 4 parts of flaxseed, 1 part of Kochia scoparia, and 1 part of Tropaeolum majus.

[0051] Example 3

[0052] An oil-control and soothing composition is obtained by compound extraction with 1 part of Vaccaria segetalis, 5 parts of flaxseed, 2 parts of Kochia scoparia, and 0.5 part of Tropaeolum majus.

[0053] Example 4

[0054] An oil-control and soothing composition is obtained by compound extraction with 3 parts of Vaccaria segetalis, 1 part of flaxseed, 1.5 parts of Kochia scoparia, and 1.5 parts of Tropaeolum majus.

[0055] Example 5

[0056] An oil-control and soothing composition is obtained by compound extraction with 2 parts of Vaccaria segetalis, 1 part of flaxseed, 2 parts of Kochia scoparia, and 0.9 part of Tropaeolum majus.

[0057] Example 6

[0058] An oil-control and soothing composition is obtained by compound extraction with 3 parts of Vaccaria segetalis, 3 parts of flaxseed, 3 parts of Kochia scoparia, and 3 parts of Tropaeolum majus.

[0059] Comparative Example 1

[0060] An oil-control and soothing composition is obtained by compound extraction with 4 parts of Vaccaria segetalis, 4 parts of flaxseed, and 4 parts of Kochia scoparia.

[0061] Comparative Example 2

[0062] An oil-control and soothing composition is obtained by compound extraction with 4.5 parts of Vaccaria segetalis, 4.5 parts of flaxseed, and 3 parts of Kochia scoparia.

[0063] Comparative Example 3

[0064] An oil-control and soothing composition is obtained by compound extraction with 4 parts of Vaccaria segetalis, 4 parts of flaxseed, and 4 parts of Tropaeolum majus.

[0065] Comparative Example 4

[0066] An oil-control and soothing composition is obtained by compound extraction with 4.5 parts of Vaccaria segetalis, 4.5 parts of flaxseed, and 3 parts of Tropaeolum majus.

[0067] Comparative Example 5

[0068] An oil-control and soothing composition is obtained by compound extraction with 4 parts of flaxseed, 4 parts of Kochia scoparia, and 4 parts of Tropaeolum majus.

[0069] Comparative Example 6

[0070] An oil-control and soothing composition is obtained by compound extraction of 4.5 parts of flaxseed, 4.5 parts of Kochia scoparia, and 3 parts of nasturtium.

[0071] Comparative Example 7

[0072] An oil-control and soothing composition is obtained by compound extraction of 4.5 parts of flaxseed, 3 parts of Kochia scoparia, and 4.5 parts of nasturtium.

[0073] Comparative Example 8

[0074] An oil-control and soothing composition is obtained by compound extraction of 4 parts of Vaccaria segetalis, 4 parts of Kochia scoparia, and 4 parts of nasturtium.

[0075] Comparative Example 9

[0076] An oil-control and soothing composition is obtained by compound extraction of 3 parts of witch hazel, 3 parts of flaxseed, 3 parts of Kochia scoparia, and 3 parts of nasturtium.

[0077] Comparative Example 10

[0078] An oil-control and soothing composition is obtained by compound extraction of 3 parts of Vaccaria segetalis, 3 parts of olive leaf, 3 parts of Kochia scoparia, and 3 parts of nasturtium.

[0079] Comparative Example 11

[0080] An oil-control and soothing composition is obtained by compound extraction of 3 parts of Vaccaria segetalis, 3 parts of flaxseed, 3 parts of Sophora flavescens, and 3 parts of nasturtium.

[0081] Comparative Example 12

[0082] An oil-control and soothing composition is obtained by compound extraction of 3 parts of Vaccaria segetalis, 3 parts of flaxseed, 3 parts of Kochia scoparia, and 3 parts of rosemary.

[0083] Efficacy Test

[0084] I. 5α-Reductase Inhibition Rate Test

[0085] Test Method:

[0086] 1 Test Purpose and Principle

[0087] The sebaceous gland secretion function of oily skin is relatively strong, the face feels greasy, is not easy to clean, and has shiny oil that affects beauty;

[0088] At the same time, some corresponding skin diseases are also likely to occur, such as acne vulgaris and seborrheic dermatitis, etc. In view of the relationship between 5α-reductase and sebaceous glands, the inhibitory effect of the test sample on 5α-reductase is usually detected to reflect the role of sebum regulation and to characterize the oil-control efficacy of the test sample.

[0089] This test refers to laboratory methods and compares the 5α-reductase inhibition rate test results of the test sample with those of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P < 0.05), it can be considered that the test sample has a certain oil control effect.

[0090] This test method is an in vitro method and is applicable to evaluating cosmetics that claim to achieve an oil control effect by inhibiting 5α-reductase.

[0091] 2 Test indicators

[0092] 5α-reductase inhibition rate determination criterion: If the 5α-reductase inhibition rate of the sample is higher than that of the negative control and there is a significant difference (P < 0.05), it can be considered that the test sample has a certain oil control effect.

[0093] 3 Test materials and methods

[0094] Test materials:

[0095] Testosterone, 98%;

[0096] NADPH, 90%;

[0097] Methanol, HPLC grade;

[0098] Buffer: Tris-HCl.

[0099] Test methods

[0100] (1)Treatment of control substances and test samples

[0101] Sample group: Dilute with buffer to a sample concentration of 5%;

[0102] Positive control substance (finasteride, purity ≥ 98%): Dilute with methanol to a positive control substance concentration of 0.1%;

[0103] Negative control substance: Tris-HCl buffer.

[0104] (2)Test operation steps

[0105] Set up a sample group, a positive control group, a negative control group, and a blank group. Each group needs to have 3 parallels. Add different reagent solutions to the four groups respectively, shake well, perform HPLC analysis on each group, and measure the testosterone content in each tube.

[0106] (3)Calculation formula

[0107] 。

[0108] (4)Data analysis

[0109] The statistical analysis software was SPSS. The independent samples t-test was used to compare the 5α-reductase inhibition rates among the test samples, positive control substances, and negative control substances. The above statistical analyses were all two-tailed tests, and the significance level was α = 0.05. P > 0.05 indicates no significant difference between the two groups; P < 0.05 indicates a significant difference between the two groups.

[0110] The oil-control and soothing compositions of Examples 1-6 and Comparative Examples 1-12 were tested according to the above method, and the results are shown in Table 1.

[0111] Table 1 Test results of 5α-reductase inhibition rates of the oil-control and soothing compositions of Examples 1-6 and Comparative Examples 1-12

[0112] 5α-Reductase Inhibition Rate % P Value Example 1 33.645 P<0.05 Example 2 36.198 P<0.05 Example 3 32.957 P<0.05 Example 4 33.211 P<0.05 Example 5 33.645 P<0.05 Example 6 35.799 P<0.05 Comparative Example 1 22.634 P<0.05 Comparative Example 2 24.268 P<0.05 Comparative Example 3 19.823 P<0.05 Comparative Example 4 22.463 P<0.05 Comparative Example 5 16.106 P<0.05 Comparative Example 6 16.529 P<0.05 Comparative Example 7 16.288 P<0.05 Comparative Example 8 16.430 P<0.05 Comparative Example 9 16.731 P<0.05 Comparative Example 10 16.912 P<0.05 Comparative Example 11 18.498 P<0.05 Comparative Example 12 18.335 P<0.05 Positive Control 66.916 P<0.05 Negative Control 2.033 /

[0113] It can be seen from the results in Table 1 that:

[0114] From the data of Examples 1-6, it can be known that in this application, the combination of semen vaccariae extract, flaxseed extract, Kochia scoparia extract, and Tropaeolum majus extract can make the 5α-reductase inhibition rate of the composition reach more than 32%, with excellent oil-control efficacy, and the effect of Example 2 is relatively better.

[0115] By comparing the data of Example 6 and Comparative Example 1, it can be seen that in the case of Comparative Example 1 lacking Tropaeolum majus, even if the total amount of raw materials is the same, the 5α-reductase inhibition rate of the composition formed by the obtained compound extract shows a significant decrease compared with Example 1; it shows that Tropaeolum majus plays an important role in the technical solution of this application; similarly, by comparing the data of Example and Comparative Example 3, it can be seen that in the case of lacking Kochia scoparia, even if the total amount of raw materials is the same, the 5α-reductase inhibition rate of the composition formed by the obtained compound extract shows a significant decrease compared with Example 1; similarly, the technical solutions of Comparative Example 5 lacking semen vaccariae and Comparative Example 8 lacking flaxseed also show a significant downward trend; thus, it can be seen that the technical solution of this application has a synergistic effect of improving the 5α-reductase inhibition rate of the composition through the combination of semen vaccariae extract, flaxseed extract, Kochia scoparia extract, and Tropaeolum majus extract.

[0116] By comparing the data of Comparative Example 1 and Comparative Example 2, it can be seen that under the same total amount, slightly reducing the amount of Kochia scoparia and correspondingly increasing the amounts of semen vaccariae and flaxseed, the 5α-reductase inhibition rate shown by the composition has a certain increase. It is speculated that one of the reasons is as follows:

[0117] (1) The 5α-reductase inhibition rates of semen vaccariae and flaxseed themselves are significantly higher than that of Kochia scoparia. Therefore, there is a certain increase after adjusting the amounts.

[0118] (2) Vaccaria segetalis and linseed have certain compound synergy, but the compound synergy is not obvious enough. Therefore, after adjusting the dosage, the synergy is further improved, thereby enhancing the 5α-reductase inhibition rate of the composition.

[0119] To verify which reason it is, in Comparative Example 6 of this application, the technical solution of increasing the dosages of linseed and Kochia scoparia in Comparative Example 5 is used to verify the differences brought by it. According to the data comparison between Comparative Example 6 and Comparative Example 5, it can be seen that in Comparative Example 6, increasing the dosage of linseed and synchronously increasing the dosage of Kochia scoparia cannot significantly enhance the 5α-reductase inhibition rate of the composition; combined with the data comparison between Comparative Example 8 and Comparative Example 5, it can be known that the 5α-reductase inhibition rate exhibited by Vaccaria segetalis is not much higher than that of linseed. Thus, it can be determined that Vaccaria segetalis and linseed have certain compound synergy, but the compound synergy is not obvious enough.

[0120] At the same time, according to the data comparison between Comparative Example 3 and Comparative Example 4, it can be seen that under the condition of the same total dosage, slightly reducing the dosage of Tropaeolum majus and correspondingly increasing the dosages of Vaccaria segetalis and linseed, the 5α-reductase inhibition rate exhibited by the composition has a certain increase; and it should be noted that the increase amplitude of the 5α-reductase inhibition rate exhibited by Comparative Example 4 compared with Comparative Example 3 is much higher than the increase amplitude of the 5α-reductase inhibition rate exhibited by Comparative Example 2 compared with Comparative Example 1. Based on this, there is reason to guess that the effect of Tropaeolum majus itself in inhibiting 5α-reductase is very weak.

[0121] Thus, Comparative Example 7 also indirectly verifies this conclusion with Comparative Example 5 and Comparative Example 6. Comparative Example 7 increases the dosage of Tropaeolum majus and reduces the dosage of Kochia scoparia compared with Comparative Example 6, but the 5α-reductase inhibition rate exhibited by the composition has a certain decrease; and in Comparative Example 7, when the dosage of linseed is higher than that in Comparative Example 5, the 5α-reductase inhibition rate exhibited by it has no improvement. Thus, it can be seen that the effect of Tropaeolum majus itself in inhibiting 5α-reductase is very weak, at least much lower than that of Kochia scoparia and linseed.

[0122] However, according to the data comparison between Example 6 and Comparative Example 1 and Comparative Example 2, it can be known that even though the effect of Tropaeolum majus itself in inhibiting 5α-reductase is very weak, through the compounding of Vaccaria segetalis, linseed, Kochia scoparia, and Tropaeolum majus, the 5α-reductase inhibition effect exhibited by it can be much higher than the compounding of Vaccaria segetalis, linseed, and Kochia scoparia, which fully shows that the compounding of extracts of Vaccaria segetalis, linseed, Kochia scoparia, and Tropaeolum majus has a synergistic effect in enhancing the 5α-reductase inhibition of the composition.

[0123] According to the data comparison of Example 6 and Comparative Examples 9-12, it can be seen that in the absence of any one of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus, even if Witch Hazel similar to the efficacy of Vaccaria segetalis is used to replace Vaccaria segetalis, Olea europaea leaf similar to the efficacy of Linum usitatissimum is used to replace Linum usitatissimum, Sophora flavescens similar to the efficacy of Kochia scoparia is used to replace Kochia scoparia, and Rosmarinus officinalis similar to the efficacy of Tropaeolum majus is used to replace Tropaeolum majus, the 5α-reductase inhibition rates shown by the compositions of Comparative Examples 9-12 all show a significant decrease, indicating that the present application shows a synergistic improvement in the 5α-reductase inhibitory effect of the composition through the compounding of extracts of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus, and none of the four can be missing; further comparing Comparative Example 11, Comparative Example 12 with Comparative Example 9 and Comparative Example 10 carefully, it can be seen that there will be a certain improvement when Vaccaria segetalis and Linum usitatissimum exist simultaneously in Comparative Example 11 and Comparative Example 12. Combining with the previous experiments, it can be more certain that there is a certain compounding synergy between Vaccaria segetalis and Linum usitatissimum, but the compounding synergy effect is not obvious enough.

[0124] II. Hyaluronidase Inhibition Rate Test

[0125] 1 Test Purpose and Principle

[0126] Hyaluronidase is a specific lyase of hyaluronic acid and is a participant in allergic reactions, with a strong correlation with the release of histamine by mast cells. Whether the test sample has a soothing effect can be judged by the hyaluronidase inhibition rate. The higher the hyaluronidase inhibition rate, the stronger the soothing effect of the substance, and vice versa.

[0127] In this test, referring to the laboratory method (HMC-WI-029 Hyaluronidase Inhibition Rate), the hyaluronidase inhibition rate test results of the test sample are compared with those of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain soothing effect.

[0128] This test method is an in vitro method and is applicable to evaluating cosmetics that claim to achieve a soothing effect by inhibiting hyaluronidase.

[0129] 2 Test Index

[0130] Hyaluronidase Inhibition Rate Judgment Standard: If the hyaluronidase inhibition rate of the sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain soothing effect.

[0131] 3 Test Materials and Methods

[0132] 3.1 Instrument and Equipment:

[0133] BSA224S Analytical Balance;

[0134] L6s Ultraviolet Spectrophotometer

[0135] 3.2 Reagents

[0136] Hyaluronidase, BR

[0137] Sodium Hyaluronate, BR

[0138] 3.3 Test Methods

[0139] (1)Treatment of Control and Test Samples

[0140] Sample Group: Dilute with pure water to a sample concentration of 5%;

[0141] Positive Control (Dipotassium Glycyrrhizinate, purity ≥ 98%): Dilute with water to a positive control concentration of 3%;

[0142] Negative Control: Pure water

[0143] (2)Test Operation Steps

[0144] Set up a sample group, a sample background group, a solvent group and a solvent background group. Each group needs to have 3 parallels. Add different reagent solutions to the four groups respectively, shake well, let stand at room temperature for 30 min for color development, and measure the absorbance value at a wavelength of 528 nm with an ultraviolet spectrophotometer.

[0145] (3)Calculation Formula

[0146] 。

[0147] In the formula: A — is the absorbance of the reaction solution without the sample;

[0148] B — is the absorbance of the reaction solution without the sample and the enzyme;

[0149] C — is the absorbance of the reaction solution containing the sample and the enzyme;

[0150] D — is the absorbance of the reaction solution containing the sample and without the enzyme.

[0151] (4)Data Analysis

[0152] The statistical analysis software is SPSS. The independent samples t-test is used to compare the hyaluronidase inhibition rates of the test samples, positive controls and negative controls.

[0153] The above statistical analyses are all two-tailed tests, and the significance level is α = 0.05. P > 0.05 indicates no significant difference between the two groups; P < 0.05 indicates a significant difference between the two groups.

[0154] The oil-control and soothing compositions of Examples 1-6 and Comparative Examples 1-12 were tested according to the above method, and the results are shown in Table 2.

[0155] Table 2 Test results of hyaluronidase inhibition rate of the oil-control and soothing compositions of Examples 1-6 and Comparative Examples 1-12

[0156] Hyaluronidase Inhibition Rate % P Value Example 1 52.604 P<0.05 Example 2 53.399 P<0.05 Example 3 53.861 P<0.05 Example 4 54.947 P<0.05 Example 5 53.258 P<0.05 Example 6 57.582 P<0.05 Comparative Example 1 48.314 P<0.05 Comparative Example 2 47.047 P<0.05 Comparative Example 3 45.662 P<0.05 Comparative Example 4 44.878 P<0.05 Comparative Example 5 50.470 P<0.05 Comparative Example 6 48.915 P<0.05 Comparative Example 7 48.034 P<0.05 Comparative Example 8 49.237 P<0.05 Comparative Example 9 47.463 P<0.05 Comparative Example 10 46.575 P<0.05 Comparative Example 11 50.686 P<0.05 Comparative Example 12 46.932 P<0.05 Positive Control 68.338 P<0.05 Negative Control -0.528 /

[0157] It can be seen from the results in Table 2 that:

[0158] From the data of Examples 1-6, it can be known that the oil-control and soothing composition of the present invention has an excellent hyaluronidase inhibition rate, but its effect varies greatly according to the dosage fluctuations among the components, and the scheme of Example 6 has a better effect.

[0159] From the data comparison between Example 6 and Comparative Examples 1-8, it can be known that in the case of lacking any one of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus, the hyaluronidase inhibition rate of the prepared composition decreases significantly, indicating that the combination of extracts of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus in this application also has the effect of synergistically enhancing the soothing effect of the composition; however, from the data of Comparative Example 5 and Comparative Example 8, it can be known that the synergistic effect of the composition of this application in terms of soothing effect is lower than that of 5α-reductase inhibition; combining the data comparison between Example 1 and Example 6, it can be known that the synergistic effect of enhancing the hyaluronidase inhibition rate by the combination of extracts of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus in this application is relatively limited, but still has a synergistic effect.

[0160] By comparing the data of Comparative Examples 1-8, it can be known that in the case of lacking any one of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus, the prepared composition only has the effect of additive efficacy; especially from the data comparison between Comparative Example 1 and Comparative Example 2, the data comparison between Comparative Example 3 and Comparative Example 4, the data comparison between Comparative Example 5 and Comparative Examples 1-4, the data comparison between Comparative Example 5 and Comparative Example 6, and the data comparison between Comparative Example 7 and Comparative Example 8, it can be inferred that: in terms of soothing effect, the efficacy ranking of the extracts of the four plants is Kochia scoparia > Tropaeolum majus > Linum usitatissimum > Vaccaria segetalis.

[0161] From the data comparison between Comparative Example 1 and Comparative Examples 9-12, it can be known that the combination of extracts of Vaccaria segetalis, Linum usitatissimum, Kochia scoparia, and Tropaeolum majus in this application has a synergistic effect of enhancing the hyaluronidase inhibition rate, and other plants cannot obtain the corresponding synergistic effect of enhancing the soothing effect; however, from the data of Comparative Example 11, it can be known that Sophora flavescens shows a hyaluronidase inhibition rate not weaker than that of Kochia scoparia.

[0162] III. Irritation test

[0163] Test method: Chicken embryo chorioallantoic membrane test

[0164] 1. Test purpose and principle

[0165] The chicken embryo chorioallantoic membrane test is an early adopted in vitro evaluation method for eye irritation. The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. This test utilizes the characteristics of the intact, clear, and transparent vascular system of the chorioallantoic membrane in the mid-stage of incubated chicken embryos. A certain amount of the test substance is directly contacted with the allantoic membrane of the chicken embryo. After acting for a period of time, the changes in the toxicity effect indicators of the chorioallantoic membrane (such as bleeding, coagulation, and vascular lysis) are observed. These indicators reflect the changes in the morphological structure, color, and permeability of blood vessels and vascular networks, as well as the phenomena of protein denaturation and the degree of damage of the chorioallantoic membrane. Then, a score is combined to evaluate the eye irritation of the test substance.

[0166] The purpose of this test is to test the ability of the test substance to cause toxicity changes in the chicken embryo chorioallantoic membrane and evaluate the elements and processes of the potential eye irritation of the substance to be evaluated.

[0167] 2. Treatment of test groups and reagents

[0168] Sample group: The oil-control and soothing compositions prepared in Examples 1-6 and Comparative Examples 1-12 are diluted into 2% aqueous solutions;

[0169] Negative control: 0.9% normal saline;

[0170] Positive control: 1% sodium dodecyl sulfate (SDS) solution.

[0171] 3. Test steps

[0172] For this test, 6 chicken embryos are selected for each group. The situation of the chorioallantoic membrane is recorded with a photographing device. The polytetrafluoroethylene resin ring is placed on the chorioallantoic membrane of the chicken embryo and photographed. The test sample to be measured is added into the polytetrafluoroethylene resin ring, the time of adding the sample is recorded, and the air chamber is covered with a moistened plastic wrap. The chicken embryo is transferred to an incubator with constant temperature and humidity for cultivation, and the degree of change of each toxicity effect is observed.

[0173] 4. Result calculation

[0174] The endpoint evaluation method is adopted for the test, and the endpoint evaluation (ES) is calculated, and the result is reserved to two decimal places; the score of each chicken embryo is the sum of the bleeding, coagulation, and vascular lysis degrees observed in each chicken embryo; ES is the average value of the mathematical sum obtained from 6 chicken embryos.

[0175] The average score of ES is calculated according to the following formula:

[0176] .

[0177] 5. Result judgment criteria

[0178] ES ≤ 4, non-irritating;

[0179] 4 < ES ≤ 12, slightly irritating;

[0180] 12 < ES < 16, moderately irritating;

[0181] ES ≥ 16, strongly irritating / corrosive.

[0182] II. Test results

[0183] Samples of Examples 1-6 and Comparative Examples 1-12 were sent for testing according to the above test method, and the ES values were all less than 4, and the results were all judged as non-irritating.

[0184] Among them, the results of the chicken embryos before and after the use of Example 6 are as Figure 1 、 Figure 2 shown;

[0185] It proves that the technical solution of the present invention is mild and non-irritating.

[0186] Application example

[0187] A shampoo, the formulation ratio is shown in Table 3, and it is prepared according to the following steps:

[0188] 1. Clean and disinfect the required equipment and utensils;

[0189] Pre-prepared phase: Phase D is pre-dispersed evenly; Phase E is pre-dissolved completely;

[0190] 2. After cleaning the pot, add pure water of Phase A, add JK-140E under homogenization conditions, after homogenously dispersing, raise the temperature to 62 ± 2 °C and add anhydrous citric acid, and keep stirring at 80-85 °C for 15-20 min;

[0191] 3. Add each raw material of Phase B in turn under the condition of 82 ± 2 °C, keep stirring for 30 min until completely dissolved and then start to cool down;

[0192] 4. When starting to cool down, add Phase C to the emulsifying pot and stir to dissolve evenly;

[0193] 5. When the temperature drops to 60 °C, add the pre-dispersed raw materials of Phase D and stir evenly;

[0194] 6. When the temperature drops to 45 °C, add the pre-dispersed Phase E and Phase F to the emulsifying pot in turn, disperse and dissolve, and stir evenly.

[0195] Table 3 Formulation ratio table

[0196]

[0197] The embodiments presented herein are merely a selection of implementation manners according to combinations of all possible embodiments. The appended claims should not be limited by the embodiments that illustrate the present invention. Some numerical ranges used in the claims include sub-ranges within them, and variations within these ranges should also be covered by the appended claims.

Claims

1. An oil-control and soothing composition, characterized in that, It includes semen vaccariae extract, linseed extract, fructuskochiae extract and tropaeolum majus extract. The semen vaccariae extract, linseed extract, fructuskochiae extract and tropaeolum majus extract are obtained by compound extraction of semen vaccariae, linseed, fructuskochiae and tropaeolum majus through the following steps: Step 1: Add semen vaccariae, linseed, fructuskochiae and tropaeolum majus into water, stir at a temperature of 60 - 70 °C for 3 - 4 h to obtain a mixture, and centrifuge the mixture to obtain an extract; Step 2: Concentrate the extract at 65 ± 5 °C and -0.06 to -0.1 MPa to 20 - 30% of the original mass to obtain a concentrated solution; Step 3: Add an equal mass of 95% ethanol to the concentrated solution for alcohol precipitation treatment, and perform solid-liquid separation to obtain an alcohol-precipitated solution; Step 4: Transfer the alcohol-precipitated solution to a falling film concentrator, and under a concentration temperature of 60 ± 5 °C and a concentration pressure of -0.06 to -0.1 MPa, vacuum concentrate the filtrate until the ethanol content is zero to obtain a non-alcohol concentrated solution; Step 5: Add butanediol and pure water to the non-alcohol concentrated solution for ultrafiltration to obtain an ultrafiltrate. The addition amount of butanediol is 1 - 15 times the mass of the non-alcohol concentrated solution, and the addition amount of pure water is 2 - 20 times the mass of the non-alcohol concentrated solution; The weight ratio of semen vaccariae, linseed, fructuskochiae and tropaeolum majus is 1 - 3:1 - 5:0.5 - 2:0.1 - 1.

5.

2. The oil-control and soothing composition according to claim 1, characterized in that, In step 5, the pore size of the ultrafiltration membrane for ultrafiltration is 0.05 - 0.45 μm, the ultrafiltration pressure is 0.1 - 0.3 MPa, and the ultrafiltration temperature is 30 ± 10 °C.

3. The oil-control and soothing composition according to claim 1, wherein It further includes step 6: Add a preservative to the ultrafiltrate and sterilize.

4. The oil-control and soothing composition according to claim 3, wherein The preservative is at least one of p-hydroxyacetophenone, hexylene glycol, octanohydroxamic acid, ethylhexylglycerin, pentylene glycol, sodium benzoate, phenoxyethanol.

5. Use of the oil-control and soothing composition as described in any one of claims 1 - 4 for preparing a washing and care product.

6. Use of the oil-control and soothing composition according to claim 5 for preparing a washing and care product, characterized in that, The washing and care product includes shampoo, hair cream, hair conditioner, essence, hair care essential oil, dry hair spray.

7. Use of the oil-control and soothing composition according to claim 5 for preparing a washing and care product, characterized in that, The washing and care product includes lotion, cream.

8. A washing and care product, characterized in that, It contains 0.1 - 30 wt% of the oil-control and soothing composition as described in any one of claims 1 - 4.

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