Anti-wrinkle firming compound fermentation extracting solution as well as preparation method and application thereof
The anti-wrinkle firming composite fermentation extract prepared by using the compound combination of Agaricus bisporus, Madhya, Chindeca and Kirin leaves, and the compound fermentation of yeast Jedinaceae Berlindner, Lactobacillus casei and Bacillus amyloidus, has solved the problem of strong irritation and poor effect of existing anti-wrinkle skin care products, and achieved significant anti-wrinkle, anti-aging and whitening effects.
Patent Information
- Application Number
- CN202510078360.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-17
- Publication Date
- 2025-04-25
AI Technical Summary
The existing anti-wrinkle skin care ingredients such as vitamin C derivatives and retinols have problems such as strong irritation and poor long-term use. Although plant extracts can reduce irritation, their effects are relatively average and have slow results, making it difficult to meet the needs of rapid wrinkle removal.
The combination of Agaricus bisporus, madder, cherry cabbage and kirin leaves was used as raw materials, and fermented by the combination of yeast Jedinaceae, Lactobacillus casei and Bacillus amyloidus to prepare an anti-wrinkle firming complex fermentation extract.
It significantly improves the anti-wrinkle and anti-aging effects, can quickly remove wrinkles, restore the skin to smoothness, and has significant whitening effects, achieving the dual effects of anti-aging and whitening.
Smart Images
Figure BDA0005247767770000071
Abstract
Description
Technical Field
[0001] The invention relates to the field of skin care product raw materials, and in particular to an anti-wrinkle and firming composite fermentation extract, a preparation method thereof, and applications thereof. Background Art
[0002] In recent years, with the growing demand for anti-aging products, various anti-wrinkle and firming skin care products have appeared on the market. However, although traditional anti-wrinkle ingredients such as vitamin C derivatives and retinols have certain effects, they generally have problems such as strong irritation and poor long-term effects.
[0003] With the development of plant extraction technology, more and more skin care products are using natural plant extracts to replace chemical synthetic ingredients, which can effectively reduce the irritation of skin care products and have certain anti-aging effects.
[0004] However, although skin care products on the market that add plant extracts can have certain anti-wrinkle effects, the effects are relatively general and slow to take effect, which can hardly meet consumers' needs for quick wrinkle removal. Therefore, there is still room for improvement. Summary of the invention
[0005] In order to improve the anti-wrinkle effect of skin care products, the present application provides an anti-wrinkle and firming composite fermentation extract, a preparation method thereof, and applications thereof.
[0006] In a first aspect, the present application provides a method for preparing an anti-wrinkle and firming composite fermentation extract, using the following technical solution: A method for preparing an anti-wrinkle and firming composite fermentation extract comprises the following steps: Step 1), putting the plant raw material into water, heating and extracting, to obtain a pre-extract; Step 2), adding composite bacteria to the pre-extract, fermenting, and obtaining a fermentation product; Step 3), filtering and sterilizing the fermented product to obtain a filtrate; Step 4), concentrating the filtrate to obtain an anti-wrinkle and firming composite fermentation extract; The plant raw materials are a combination of Agaricus bisporus, Rubia cordifolia, Lythrum saussurea var. The composite bacteria is a combination of Saccharomyces cerevisiae, Lactobacillus paracasei and Bacillus amyloliquefaciens.
[0007] By adopting the above technical scheme, by using a combination of Agaricus bisporus, Rubia cordifolia, Lythrum sachalinensis and Euphorbia cerevisiae as raw materials, and fermenting with a composite bacteria formed by a combination of Saccharomyces cerevisiae, Lactobacillus paracasei and Bacillus amyloliquefaciens, the active ingredients and the fermentation products cooperate with each other to produce significant anti-wrinkle and anti-aging effects, which can quickly remove wrinkles, restore skin smoothness, and has a significant whitening effect, and can achieve the anti-aging and whitening effects at the same time.
[0008] Preferably, the mass ratio of the Agaricus bisporus, Rubia cordifolia, Lythrum saussurea and Euphorbia trichosanthis is 10-12:3-5:2-4:5-7.
[0009] By adopting the above technical scheme and specifically selecting the mass ratio of Agaricus bisporus, Rubia cordifolia, Lythrum saussurea and Euphorbia trichosanthis, the active ingredients can cooperate with each other better, and the anti-aging and anti-wrinkle effects can be better.
[0010] Preferably, the mass ratio of the yeast Jendingseberlindnerella, Lactobacillus paracasei and Bacillus amyloliquefaciens is 1-2:1-2:1-2.
[0011] By adopting the above technical scheme and specifically selecting the mass ratio of Saccharomyces cerevisiae, Lactobacillus paracasei and Bacillus amyloliquefaciens, the fermentation product and the active ingredients can cooperate with each other better, which can better improve the anti-aging and whitening effects.
[0012] Preferably, the mass ratio of the plant raw material to the composite bacteria is 20-28:0.003-0.006.
[0013] By adopting the above technical scheme and specifically selecting the mass ratio of plant raw materials and composite bacteria, the fermentation can be more complete, while the cost can be effectively controlled, which has a higher economic value. The active ingredients and fermentation products can be better coordinated to exert a better synergistic effect, further improving the anti-aging and whitening effects.
[0014] Preferably, the mass ratio of the plant raw material to water is 20-28:100-120.
[0015] By adopting the above technical solution and specifically selecting the mass ratio of plant raw materials and water, the extraction efficiency is increased, the energy waste in the subsequent concentration step is reduced, the process cost is effectively controlled, and the economic value is high.
[0016] Preferably, the mass ratio of the plant raw material to the anti-wrinkle and firming composite fermentation extract is 20-28:40-56.
[0017] By adopting the above technical scheme and specifically selecting the mass ratio of plant raw materials and anti-wrinkle and firming composite fermentation extract, the concentration of active ingredients and fermentation products in the anti-wrinkle and firming composite fermentation extract is just right, and the amount added to skin care products is easier to match with the skin care product formula, reducing the difficulty of the skin care product production process and making the anti-wrinkle and firming composite fermentation extract easier to use.
[0018] Preferably, in the step 1), during the heating extraction, the temperature is kept constant at 80-85° C., condensed and refluxed, and the extraction is performed for 7-8 hours.
[0019] By adopting the above technical solution and specifically selecting a constant temperature of 80-85°C for extraction for 7-8h, the extraction effect is better, and the damage of high temperature to active ingredients is reduced, so that the product quality is better.
[0020] Preferably, in step 2), during fermentation, the temperature is kept at 28-30° C. and the fermentation lasts for 6-7 days.
[0021] By adopting the above technical solution and specifically selecting a constant temperature of 28-30°C for fermentation for 6-7 days, the activity of the composite bacteria is higher, the fermentation effect is better, and the product quality is better.
[0022] In a second aspect, the present application provides an anti-wrinkle and firming composite fermented extract, which adopts the following technical solution: An anti-wrinkle and firming composite fermentation extract is prepared by the above-mentioned preparation method of the anti-wrinkle and firming composite fermentation extract.
[0023] By adopting the above technical scheme, the anti-wrinkle and firming composite fermentation extract has excellent anti-wrinkle, anti-aging and whitening effects, and can repair the skin well, making the skin smooth and healthy again.
[0024] In the third aspect, the present application provides an application of an anti-wrinkle and firming composite fermentation extract, adopting the following technical scheme: an application of the above-mentioned anti-wrinkle and firming composite fermentation extract, characterized in that: the anti-wrinkle and firming composite fermentation extract is added to skin care products to prepare skin care products with anti-wrinkle and firming effects, and the mass content of the anti-wrinkle and firming composite fermentation extract in the skin care products is 20-30%.
[0025] By adopting the above technical solution, the prepared skin care products can not only play the original functions of skin care products, but also have good anti-wrinkle, anti-aging and whitening effects, so that the skin care products can repair the skin more comprehensively and make the skin healthier.
[0026] In summary, this application has the following beneficial effects: 1. Since the present application adopts a compound of Agaricus bisporus, Rubia cordifolia, Lythrum sachalinensis, and Euphorbia cerevisiae as raw materials, and is fermented by a composite bacteria formed by a compound of Saccharomyces cerevisiae, Lactobacillus paracasei, and Bacillus amyloliquefaciens, the active ingredients and the fermentation products cooperate with each other to produce significant anti-wrinkle and anti-aging effects, can quickly remove wrinkles, restore skin to smoothness, and has a significant whitening effect, and can achieve the anti-aging and whitening effects at the same time.
[0027] 2. In the present application, it is preferred to specifically select the mass ratio of Agaricus bisporus, Rubia cordifolia, Lythrum saussurea and Euphorbia trichosanthis so that the active ingredients cooperate with each other better and the anti-aging and anti-wrinkle effects are better.
[0028] 3. In the present application, it is preferred to specifically select the mass ratio of Saccharomyces cerevisiae, Lactobacillus paracasei, and Bacillus amyloliquefaciens so that the fermentation product and the active ingredients cooperate with each other better, which can better improve the anti-aging and whitening effects. DETAILED DESCRIPTION
[0029] The present application is further described in detail below with reference to the embodiments.
[0030] Example 1 An anti-wrinkle and firming composite fermentation extract, the preparation method of the anti-wrinkle and firming composite fermentation extract is as follows: Step 1), 10kg of Agaricus bisporus, 3kg of Rubia cordifolia, 2kg of Lythrum saussurea, 5kg of Euphorbia cerasifera and 100kg of water are put into a stirring tank, heated and kept at a constant temperature of 80°C, condensed and refluxed, and extracted for 7h to obtain a pre-extract.
[0031] Step 2), after the pre-extract is cooled to room temperature, 1 g of Yeast Jardine Seberlindner, 1 g of Lactobacillus paracasei, and 1 g of Bacillus amyloliquefaciens are added to the pre-extract, and the mixture is fermented at a constant temperature of 28° C. for 6 days to obtain a fermented product.
[0032] Step 3), the fermented product is filtered through a 200-mesh filter cloth to remove the filter residue, and then filtered through a 0.22 μm filter membrane for sterilization to obtain a filtrate.
[0033] Step 4), the filtrate is concentrated by rotary evaporation at a rotary evaporation temperature of 50° C. and a rotary evaporation pressure of 0.01 MPa to obtain 40 kg of anti-wrinkle and firming composite fermentation extract.
[0034] Agaricus bisporus is from commercially available, dry product.
[0035] Rubia cordifolia was purchased from commercial sources and is a dry product.
[0036] Lythrum salicinae is from commercially available, dry product.
[0037] Kirin leaf is from commercially available, dry product.
[0038] Yeast of Jardine Seiberlin was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D13702.
[0039] Lactobacillus casei was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D24349.
[0040] Bacillus amyloliquefaciens was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D52949.
[0041] Example 2 An anti-wrinkle and firming composite fermentation extract, the preparation method of the anti-wrinkle and firming composite fermentation extract is as follows: Step 1), 11 kg of Agaricus bisporus, 4 kg of Rubia cordifolia, 3 kg of Lythrum saussurea, 6 kg of Euphorbia cerasifera, and 110 kg of water are put into a stirring tank, heated and kept at a constant temperature of 82.5° C., condensed and refluxed, and extracted for 7.5 hours to obtain a pre-extract.
[0042] Step 2), after the pre-extract is cooled to room temperature, 1.5 g of Jedinseiberlindner yeast, 1.5 g of Lactobacillus paracasei, and 1.5 g of Bacillus amyloliquefaciens are added to the pre-extract, and the mixture is fermented at a constant temperature of 29° C. for 6.5 days to obtain a fermented product.
[0043] Step 3), the fermented product is filtered through a 200-mesh filter cloth to remove the filter residue, and then filtered through a 0.22 μm filter membrane for sterilization to obtain a filtrate.
[0044] Step 4), the filtrate is concentrated by rotary evaporation at a rotary evaporation temperature of 50° C. and a rotary evaporation pressure of 0.01 MPa to obtain 48 kg of anti-wrinkle and firming composite fermentation extract.
[0045] Agaricus bisporus is from commercially available, dry product.
[0046] Rubia cordifolia was purchased from commercial sources and is a dry product.
[0047] Lythrum salicinae is from commercially available, dry product.
[0048] Kirin leaf is from commercially available, dry product.
[0049] Yeast of Jardine Seiberlin was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D13702.
[0050] Lactobacillus casei was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D24349.
[0051] Bacillus amyloliquefaciens was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D52949.
[0052] Example 3 An anti-wrinkle and firming composite fermentation extract, the preparation method of the anti-wrinkle and firming composite fermentation extract is as follows: Step 1), 12kg of Agaricus bisporus, 5kg of Rubia cordifolia, 4kg of Lythrum saussurea, 7kg of Euphorbia cerasifera, and 120kg of water are put into a stirring tank, heated and kept at a constant temperature of 85°C, condensed and refluxed, and extracted for 8h to obtain a pre-extract.
[0053] Step 2), after the pre-extract is cooled to room temperature, 2 g of Saccharomyces cerevisiae, 2 g of Lactobacillus paracasei, and 2 g of Bacillus amyloliquefaciens are added to the pre-extract, and the mixture is fermented at a constant temperature of 30° C. for 7 days to obtain a fermented product.
[0054] Step 3), the fermented product is filtered through a 200-mesh filter cloth to remove the filter residue, and then filtered through a 0.22 μm filter membrane for sterilization to obtain a filtrate.
[0055] Step 4), the filtrate is concentrated by rotary evaporation at a rotary evaporation temperature of 50° C. and a rotary evaporation pressure of 0.01 MPa to obtain 56 kg of anti-wrinkle and firming composite fermentation extract.
[0056] Agaricus bisporus is from commercially available, dry product.
[0057] Rubia cordifolia was purchased from commercial sources and is a dry product.
[0058] Lythrum salicinae is from commercially available, dry product.
[0059] Kirin leaf is from commercially available, dry product.
[0060] Yeast of Jardine Seiberlin was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D13702.
[0061] Lactobacillus casei was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D24349.
[0062] Bacillus amyloliquefaciens was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D52949.
[0063] Comparative Example 1 An anti-wrinkle and firming composite fermented extract, compared with Example 2, the only difference is: Replace Agaricus bisporus with an equal amount of olives.
[0064] Olives were commercially available, dried products.
[0065] Comparative Example 2 An anti-wrinkle and firming composite fermented extract, compared with Example 2, the only difference is: Replace madder with an equal amount of Centella asiatica.
[0066] Centella asiatica is from commercially available, dry product.
[0067] Comparative Example 3 An anti-wrinkle and firming composite fermented extract, compared with Example 2, the only difference is: Replace Lythrum salicaria with an equal amount of Globenia gracilis.
[0068] Globe amaranth is from commercially available, dry product.
[0069] Comparative Example 4 An anti-wrinkle and firming composite fermented extract, compared with Example 2, the only difference is: Use kelp in equal amounts to replace the kiwi leaf.
[0070] Kelp is from commercially available, dry product.
[0071] Comparative Example 5 An anti-wrinkle and firming composite fermented extract, compared with Example 2, the only difference is: Replace Agaricus bisporus with an equal amount of olive, replace madder with an equal amount of Centella asiatica, replace Lythrum saussurea with an equal amount of Globe theta, and replace Euphorbia trichosanthis with an equal amount of kelp.
[0072] Olives were commercially available, dried products.
[0073] Centella asiatica is from commercially available, dry product.
[0074] Globe amaranth is from commercially available, dry product.
[0075] Kelp is from commercially available, dry product.
[0076] Comparative Example 6 An anti-wrinkle and firming composite fermented extract, compared with Example 2, the only difference is: Candida glabrata was used to replace the Y. jadinseberlindnerii in equal amounts.
[0077] Candida glabrata was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D10518.
[0078] Comparative Example 7 An anti-wrinkle and firming composite fermented extract, compared with Example 2, the only difference is: Lactobacillus fermentum was used to replace Lactobacillus paracasei in equal amounts.
[0079] Lactobacillus fermentum was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D14218.
[0080] Comparative Example 8 An anti-wrinkle and firming composite fermented extract, compared with Example 2, the only difference is: Bacillus subtilis was used to replace Bacillus amyloliquefaciens in equal amounts.
[0081] Bacillus subtilis was purchased from Shanghai Biotechnology Collection Center, strain number: SHBCC D10342.
[0082] Comparative Example 9 An anti-wrinkle and firming composite fermented extract, compared with Example 2, the only difference is: Candida glabrata was used to replace Saccharomyces jadinseberlindneri in equal amounts, Lactobacillus fermentum was used to replace Lactobacillus paracasei in equal amounts, and Bacillus subtilis was used to replace Bacillus amyloliquefaciens in equal amounts.
[0083] Candida glabrata was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D10518.
[0084] Lactobacillus fermentum was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D14218.
[0085] Bacillus subtilis was purchased from Shanghai Biotechnology Collection Center, strain number: SHBCC D10342.
[0086] Experiment 1 According to T / AHPCA 047-2023 "Test Method for Anti-Wrinkle and Firming Efficacy of Cosmetics (Rapid Determination of Elastase Inhibition)", the elastase inhibition rate (%) of the test samples prepared from the anti-wrinkle and firming composite fermentation extracts of each embodiment and comparative example was tested.
[0087] Sample to be tested: Take 20g of anti-wrinkle and firming composite fermentation extract and 80g of deionized water, mix well to obtain the sample to be tested.
[0088] Experiment 2 According to T / SHRH 006-2018 "Experimental Method for Scavenging Cosmetics Free Radicals (DPPH)", the DPPH free radical scavenging rate (%) of the test sample prepared from the anti-wrinkle and firming composite fermentation extract of each embodiment and comparative example was detected.
[0089] Sample to be tested: Take 20g of anti-wrinkle and firming composite fermentation extract and 80g of deionized water, mix them evenly to obtain the sample to be tested.
[0090] Experiment 3 According to T / GDCA 006-2021 "Test method for inhibition of tyrosinase activity by cosmetic raw materials (in vitro method)", the tyrosinase inhibition rate (%) of the test samples prepared from the anti-wrinkle and firming composite fermentation extracts of each embodiment and comparative example was tested.
[0091] Sample to be tested: Take 20g of anti-wrinkle and firming composite fermentation extract and 80g of deionized water, mix well to obtain the sample to be tested.
[0092] The specific test data of Experiments 1-3 are shown in Table 1.
[0093] Table 1 According to the data comparison of each embodiment and the comparative example in Table 1, it can be obtained that the elastase inhibition rate, DPPH free radical scavenging rate, and tyrosinase inhibition rate of each embodiment are significantly higher than those of each comparative example. It can be seen that each embodiment has more significant anti-wrinkle, anti-aging, and whitening effects, can more comprehensively and efficiently care for the skin, and restore the skin to health faster.
[0094] In Comparative Examples 1-5, other plant raw materials with anti-wrinkle effects are used to replace the Agaricus bisporus, Rubia cordifolia, Lythrum sachalinensis, and Euphorbia cerana in the embodiments alone or in whole. Although they can also have certain anti-wrinkle and anti-aging effects, the special compound of Agaricus bisporus, Rubia cordifolia, Lythrum sachalinensis, and Euphorbia cerana is destroyed, resulting in a significant decrease in the effect of the various active ingredients cooperating with each other, resulting in a more obvious decrease in the anti-wrinkle and anti-aging effects compared with the embodiments, and the whitening effect is poor, making it difficult to achieve a better whitening effect.
[0095] In Comparative Examples 6-9, other fermentation bacteria are used alone or in whole to replace the Jedinseiberlindner Saccharomyces cerevisiae, Lactobacillus paracasei, and Bacillus amyloliquefaciens in the embodiments. Since the composite system of Jedinseiberlindner Saccharomyces cerevisiae, Lactobacillus paracasei, and Bacillus amyloliquefaciens is destroyed, the effect of the fermentation product and the active ingredient is reduced, resulting in the anti-aging, anti-wrinkle, and whitening effects being better than those of Comparative Examples 1-5, compared with the embodiments, the anti-aging, anti-wrinkle, and whitening effects are all reduced.
[0096] It can be seen that only when Agaricus bisporus, Rubia cordifolia, Lythrum sachalinensis, and Euphorbia cerevisiae are compounded and fermented by Saccharomyces cerevisiae, Lactobacillus paracasei, and Bacillus amyloliquefaciens, can the obtained extract exert very significant anti-wrinkle, anti-aging, and whitening effects.
[0097] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.
Claims
1. A method for preparing an anti-wrinkle and firming composite fermentation extract, characterized in that: The following steps are involved: Step 1), putting the plant raw material into water, heating and extracting, to obtain a pre-extract; Step 2), adding composite bacteria to the pre-extract, fermenting, and obtaining a fermentation product; Step 3), filtering and sterilizing the fermented product to obtain a filtrate; Step 4), concentrating the filtrate to obtain the anti-wrinkle and firming composite fermentation extract; The plant raw materials are a combination of Agaricus bisporus, Rubia cordifolia, Lythrum saussurea var. The composite bacteria is a combination of Saccharomyces cerevisiae, Lactobacillus paracasei and Bacillus amyloliquefaciens.
2. The method for preparing the anti-wrinkle and firming composite fermentation extract according to claim 1, characterized in that: The mass ratio of the Agaricus bisporus, Rubia cordifolia, Lythrum saussurea and Euphorbia trichosanthis is 10-12:3-5:2-4:5-7.
3. The method for preparing the anti-wrinkle and firming composite fermentation extract according to claim 2, characterized in that: The mass ratio of the yeast Jedinseiberlindner, Lactobacillus paracasei and Bacillus amyloliquefaciens is 1-2:1-2:1-2.
4. The method for preparing the anti-wrinkle and firming composite fermentation extract according to claim 3, characterized in that: The mass ratio of the plant raw material to the composite bacteria is 20-28:0.003-0.
006.
5. The method for preparing the anti-wrinkle and firming composite fermentation extract according to claim 1, characterized in that: The mass ratio of the plant raw material to water is 20-28:100-120.
6. The method for preparing the anti-wrinkle and firming composite fermentation extract according to claim 5, characterized in that: The mass ratio of the plant raw material to the anti-wrinkle and firming composite fermentation extract is 20-28:40-56.
7. The method for preparing the anti-wrinkle and firming composite fermentation extract according to claim 1, characterized in that: In the step 1), during the heating extraction, the temperature is kept constant at 80-85° C., condensed and refluxed, and the extraction is performed for 7-8 hours.
8. The method for preparing the anti-wrinkle and firming composite fermentation extract according to claim 1, characterized in that: In the step 2), during fermentation, the temperature is kept at 28-30° C. and the fermentation lasts for 6-7 days.
9. An anti-wrinkle and firming composite fermented extract, characterized in that: The invention is prepared by the preparation method of the anti-wrinkle and firming composite fermentation extract according to any one of claims 1 to 8.
10. An application of the anti-wrinkle and firming composite fermented extract according to claim 9, characterized in that: The anti-wrinkle and firming composite fermentation extract is added to skin care products to prepare skin care products with anti-wrinkle and firming effects. The mass content of the anti-wrinkle and firming composite fermentation extract in the skin care products is 20-30%.