Antibacterial mouthwash and preparation method thereof

Abstract

The invention belongs to the field of oral care products and provides antibacterial mouthwash which is prepared from the following raw materials in percentage by mass: 20-30% of lactobacillus plantarum powder, 25-30% of plant extract, 10-15% of humectant, 2-3% of preservative and 0.5-2% of pH regulator. 5-12% of an antibacterial synergist; 2-3% of a sweetening agent and 17-35% of deionized water; the plant extracts comprise a honeysuckle flower extract, a Philippine violet herb extract, a left-hand fragrant extract, a tea leaf extract and a licorice root extract; lactobacillus plantarum powder is provided by Shanghai Shanghai Shanghai Shanghai Detection Technology Co., Ltd., and the product number is BNCC299253. The toothpaste can play a good role in protecting helicobacter pylori, salmonella, listeria monocytogenes, enterobacter sakakaakii and the like, can relieve swelling and aching of gum, oral mucosa bleeding and other symptoms, and avoids halitosis. And the toothpaste also has a very good prevention effect on diseases such as oral ulcer, gingivitis and periodontitis.

Description

Technical Field

[0001] The invention relates to the field of oral care products, and in particular to an antibacterial mouthwash and a preparation method thereof. Background Art

[0002] Mouthwash, as an oral care product, has been widely popularized and used around the world in recent years. It can not only effectively clean the mouth, remove food residues and bad breath, but also bring a fresh and comfortable oral feeling to the user. With the increasing awareness of oral health, the mouthwash market has also ushered in rapid development. However, traditional mouthwash products often rely on chemical synthetic ingredients to achieve their antibacterial and cleaning effects, and long-term use may pose potential risks to human health.

[0003] The oral cavity is one of the environments with the richest variety of microorganisms in the human body, including a variety of pathogenic bacteria such as Candida albicans, Streptococcus, Escherichia coli, Staphylococcus, etc. The balance of these flora in the oral cavity is crucial to maintaining oral health. However, when the flora is unbalanced or the immune system is reduced, these pathogenic bacteria may cause various oral problems, such as swollen and painful gums, bad breath, oral mucosal bleeding, etc. What's more serious is that some pathogenic bacteria such as Helicobacter pylori and Salmonella may enter the human body through the oral cavity and cause systemic diseases.

[0004] In the current medical environment where antibiotics and immunosuppressants are widely used, the problems of oral flora imbalance and reduced immunity are becoming increasingly prominent. Therefore, it is particularly important to find a mouthwash product that can effectively fight bacteria and maintain the balance of oral flora while being safe and harmless to the human body.

[0005] In addition, as consumers pursue a healthy lifestyle, natural, green, and additive-free products are becoming more and more popular. The chemically synthesized ingredients in traditional mouthwashes often cannot meet consumers' demands for product safety and naturalness. Therefore, developing an antibacterial mouthwash made from natural herbs as the main raw material, through scientific proportions and advanced processes, not only meets the current market demand for health products, but is also a major innovation and development direction in the field of oral care. Summary of the invention

[0006] The present invention is aimed at the problems of existing mouthwash products, such as many chemical synthetic ingredients, potential risks in long-term use, and failure to meet consumers' demand for naturalness. An antibacterial mouthwash and preparation method are proposed, which uses pure natural herbal essences for reasonable combination to achieve antibacterial effect of the mouthwash product and its preparation method. The present invention aims to provide a safe and effective oral care solution to meet consumers' demand for healthy, natural, and green products.

[0007] The present invention is achieved through the following technical solutions: An antibacterial mouthwash is composed of the following raw materials in percentage by weight: 20-30% of plant lactobacillus powder, 25-30% of plant extract, 10-15% of moisturizer, 2-3% of preservative, 0.5-2% of pH regulator, 5-12% of antibacterial enhancer, 2-3% of sweetener, and 17-35% of deionized water; The plant extracts are honeysuckle extract, magnolia officinalis extract, Plectranthus amboinicus extract, tea extract, and liquorice extract; Lactobacillus plantarum powder is provided by Shanghai Shangjian Testing Technology Co., Ltd., and the product number is BNCC299253.

[0008] Furthermore, the raw materials are composed of the following mass percentages: 22-30% of plant lactobacillus powder, 25-30% of plant extract, 12-15% of moisturizer, 2-3% of preservative, 1.2-2% of pH adjuster, 6-12% of antibacterial enhancer, 2-3% of sweetener, and 20-35% of deionized water.

[0009] Furthermore, the raw materials are composed of the following mass percentages: 24-28% of plant lactobacillus powder, 28-30% of plant extract, 13-15% of moisturizer, 2-3% of preservative, 1.5-2% of pH adjuster, 5-10% of antibacterial enhancer, 2-3% of sweetener, and 20-30% of deionized water.

[0010] Furthermore, the raw materials are composed of the following mass percentages: 25-30% of plant lactobacillus powder, 28-30% of plant extract, 12-15% of moisturizer, 2-3% of preservative, 1.8-2% of pH adjuster, 10-12% of antibacterial enhancer, 2-3% of sweetener, and 32-35% of deionized water.

[0011] Furthermore, the raw materials are composed of the following mass percentages: 30% of plant lactobacillus powder, 28% of plant extract, 12% of moisturizer, 2% of preservative, 1.2% of pH adjuster, 6% of antibacterial enhancer, 3% of sweetener, and 30% of deionized water.

[0012] Furthermore, the plant extract is honeysuckle extract, hollyhock extract, pectinaceus extract, tea extract, and licorice extract.

[0013] Furthermore, the extraction method of honeysuckle extract is: using ultrasonic assisted extraction method, using 60% ethanol as solvent, material ratio 1:30, extraction time 458min, far-infrared microwave 1min, the extraction is obtained.

[0014] Honeysuckle is cold in nature and sweet in taste. It has the effects of clearing away heat and detoxifying, dispersing wind and dissipating heat. It is a good medicine for clearing heat and detoxifying. The compounds secologanol, swertia glycoside, canka glycoside, At a certain concentration, cordysinin B showed an inhibitory effect comparable to ampicillin on Streptococcus mutans, a common oral caries-causing bacterium. Honeysuckle extract has significant antibacterial and antibacterial effects, and has a good inhibitory effect on a variety of pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. Honeysuckle extract in mouthwash can be used to relieve symptoms such as gingivitis and oral ulcers, and also has a certain preventive effect on dental plaque and gingival bleeding.

[0015] Furthermore, the extraction method of the ponzu extract is: ethanol reflux extraction, which is performed by using ethanol as a solvent and heating under reflux.

[0016] The main active ingredient in Magnolia officinalis extract is magnolol, which has a significant antibacterial effect. Studies have shown that magnolol has a high inhibitory effect on Streptococcus mutans, a common cariogenic bacteria. Magnolia officinalis extract can not only inhibit bacterial growth, but also reduce the formation of dental plaque, thereby preventing the occurrence of caries.

[0017] Furthermore, the extraction method of the Plectranthus amboinicus extract is: using an ethanol extraction method, using 65% ethanol as a solvent, to extract.

[0018] The role of Plectranthus amboinicus extract in mouthwash is mainly reflected in its antibacterial, anti-inflammatory and expectorant effects. Plectranthus amboinicus extract can help remove excess mucus in the respiratory tract, thereby achieving an expectorant effect. By inhibiting bacterial growth, it reduces the occurrence of oral problems such as dental plaque and gingivitis.

[0019] Furthermore, the extraction method of tea extract is: microwave-assisted extraction, using a solid / liquid ratio of 1:20, and extracting at a temperature of 200-230°C for 2 minutes; after the green tea leaves are extracted for 5 minutes under controlled microwave power, they are extracted with distilled water for 5 minutes to obtain an extract.

[0020] Tea polyphenols and catechins in tea extracts have significant antibacterial activity and can inhibit the growth of pathogenic bacteria in the mouth. Green tea extract has an inhibitory effect on oral bacteria such as Staphylococcus aureus and Rhinoceros. Polyphenolic compounds in green tea extracts, such as epigallocatechin gallate (EGCG), have anti-inflammatory properties and can reduce gingival inflammation. Green tea mouthwash can effectively reduce volatile sulfide compounds (VSC) in the mouth, thereby reducing bad breath. In addition, green tea extract mouthwash can also reduce the number of bacteria in saliva, such as Streptococcus mutans. Green tea extract has a promoting effect on the healing process of oral ulcers. Green tea extract is also used to prevent dental caries and has a certain anti-caries effect. In addition, green tea mouthwash can regulate the oral environment and change saliva from acidic to neutral or alkaline, thereby helping to maintain oral health.

[0021] Furthermore, the extraction method of the licorice extract is: using 70% ethanol as a solvent, subjecting the licorice powder to ultrasonic treatment for 2 hours at 25° C., and then concentrating by vacuum evaporation to obtain a filtrate.

[0022] Licorice extract has significant antibacterial activity and can effectively inhibit the growth of bacteria in the mouth. Licorice extract has an inhibitory effect on a variety of oral bacteria such as Helicobacter pylori, Candida albicans, and Staphylococcus aureus. Its antibacterial effect is even better than some traditional mouthwash ingredients such as chlorhexidine. In addition, the active ingredients in licorice extract, such as glycyrrhetinic acid and liquiritin, can inhibit the growth and metabolism of bacteria. Licorice extract also has anti-inflammatory properties and can reduce oral inflammation. When treating oral ulcers and periodontal disease, licorice extract can relieve symptoms by inhibiting the release of inflammatory factors.

[0023] Furthermore, the moisturizer is one or more of glycerol, 1,3-butylene glycol, polyethylene glycol, and sorbitol.

[0024] Furthermore, the preservative is one or more of sodium benzoate, methylparaben, hydroxybenzoate, and potassium sorbate.

[0025] Furthermore, the pH adjuster is one or both of citric acid and benzoic acid.

[0026] Furthermore, the antibacterial synergist is one or more of menthol, thymol, and methyl salicylate.

[0027] Furthermore, the sweetener is one or more of xylitol, sorbitol, D-xylose and thaumatin.

[0028] The method for preparing the above-mentioned antibacterial mouthwash comprises the following steps: (1) inoculating Lactobacillus plantarum into MRS medium or similar medium rich in carbohydrates and nitrogen sources, activating and culturing at 30-40° C. for 24-36 hours, with a pH value of 6.0-7.5, then inoculating the activated Lactobacillus plantarum culture liquid into the MRS medium at an inoculum amount of 5% by volume, fermenting and culturing at 30-40° C. for 20-30 hours to obtain Lactobacillus plantarum fermentation liquid, sterilizing, concentrating and drying to obtain Lactobacillus plantarum powder; (2) The plant extract, moisturizer, preservative, pH adjuster, antibacterial enhancer and sweetener are mixed evenly according to a proportion, and then deionized water is added and stirred to obtain a mixed solution; (3) Add Lactobacillus plantarum powder into the mixed solution at a temperature of 30-40° C. and mix well to prepare an antibacterial mouthwash.

[0029] Further, the main components of the MRS medium include: glucose: 20 g / L, casein hydrolysate: 10 g / L, broth: 10 g / L, yeast extract: 5 g / L, sodium acetate: 5 g / L, polysorbate 80 (Tween80): 80.1 g / L, ammonium chloride: 2 g / L, dipotassium hydrogen phosphate: 2 g / L, magnesium sulfate: 0.5 g / L, manganese sulfate: 0.05 g / L, and after the pH value was adjusted to 6.5, it was sterilized by high-pressure steam at 121°C for 30 minutes.

[0030] Furthermore, a similar culture medium rich in carbohydrates and nitrogen sources is CM0006 culture medium, the components of which are: casein peptone 10.0 g / L, beef extract 10.0 g / L, yeast powder 5.0 g / L, glucose 5.0 g / L, sodium acetate 5.0 g / L, diammonium citrate 2.2 g / L, K2HPO4 1.0 g / L, MgSO4.7H2O 0.4 g / L, MnSO4.H2O 0.05 g / L, CaCO3 2.0 g / L, agar 15 g / L, distilled water 1.0 L, pH 6.8.

[0031] Compared with the existing technical solutions, it has the following beneficial effects: 1. The present invention obtains a natural antibacterial mouthwash by rationally matching natural herbal extracts, which achieves antibacterial effects on a variety of pathogenic bacteria in the oral cavity, including Helicobacter pylori, Salmonella, Listeria, Enterobacter sakazakii, etc., can relieve symptoms such as swollen and painful gums, oral mucosal bleeding, and avoid bad breath. At the same time, it also has a good preventive effect on diseases such as oral ulcers, gingivitis, and periodontitis.

[0032] 2. The main effects of the Magnolia officinalis extract in the plant extract of the present invention are to dry dampness and eliminate phlegm, to relieve gas and eliminate stagnation, and can adjust the lungs and digestive tract, especially the digestive tract. In addition, tea extracts contain rich tea polyphenols and other ingredients that can achieve the effect of preventing caries and strengthening teeth. Citric acid has a refreshing lemon fragrance, is mild and refreshing, and can freshen breath and play a role in cleaning tartar. The use of pure natural herbal plant essence avoids the side effects that may be caused by chemical synthetic ingredients, and is safer and gentler. In addition to antibacterial, it also has multiple effects such as drying dampness and eliminating phlegm, preventing caries and strengthening teeth, and refreshing breath, which comprehensively protects oral health.

[0033] 3. The present invention has developed a green and healthy mouthwash with Lactobacillus plantarum as the main ingredient to inhibit the pathogenic bacteria Streptococcus mutans. Lactobacillus plantarum can be used as a new type of safe and effective raw material for oral care cleaning liquid to prepare mouthwash and prevent the occurrence of dental caries. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is an antibacterial experiment of four antibacterial mouthwashes prepared in the embodiments of the present invention and a control group. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings and corresponding embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] The oral cavity is a bacterial environment with a large number of fungi, such as Lactobacillus, oral Candida albicans, Actinomycetes, Porphyromonas gingivalis, etc. Under normal circumstances, the oral flora will maintain a relatively dynamic equilibrium state, will not affect the body, and generally will not cause infection. However, if there is an imbalance in the flora, or the body's immunity is low, it may cause abnormal conditions, such as swollen and painful gums, bad breath, oral mucosal bleeding and other symptoms. Usually, if a bacterial infection occurs in the oral cavity, it may also cause an immune response in the body, causing oral ulcers, gingivitis, periodontitis and other diseases. In addition, some bacteria such as Helicobacter pylori, Salmonella, Listeria, and Enterobacter sakazakii can easily enter the human body through the mouth, causing some diseases.

[0037] Therefore, in addition to brushing your teeth every morning and evening and rinsing your mouth after meals, it is best to use an antibacterial mouthwash to keep your mouth clean while resisting various harmful bacteria, avoiding diseases from entering the mouth, and preventing bacteria and food residues from forming dental plaque, which further calcifies into tartar and causes diseases such as gingivitis and tartar.

[0038] In view of the problems that traditional mouthwash products have many chemical synthetic ingredients, potential risks in long-term use, and cannot meet consumers' demand for naturalness, the present invention has developed an antibacterial mouthwash that can effectively fight bacteria and maintain the balance of oral flora, while being safe and harmless to the human body, by selecting natural herbal ingredients, optimizing extraction and preparation processes, integrating multiple functions, and ensuring safety and naturalness. In the present invention, multiple functions can be achieved by adding a variety of pure plant extracts. According to the data and the experimental data made by the inventor, for example, when the content of honeysuckle extract increases from 1% to 30%, its help to gum and oral health increases significantly. When the content of Houpu extract increases from 1% to 20%, the functions of drying dampness and eliminating phlegm, lowering qi and eliminating accumulation are significant, and the lungs and digestive tract are improved, especially the digestive tract. The improvement effect will also be greatly improved as the concentration increases. When the content of tea extract reaches 0.1%, the tea polyphenols contained therein can achieve the effect of preventing caries and strengthening teeth. When the content is further increased to 30%, its effect will increase and reach a limit. When the content of licorice extract reaches 0.1%, it has obvious heat-clearing and detoxifying effects. When the content exceeds 30%, the effect of licorice extract increases and the adverse effects of licorice extract on the product will be much more obvious. However, the plant extract components will have an adverse effect on the texture of mouthwash. Therefore, the present invention can neutralize the adverse effects of the high extract components on the texture of mouthwash by adding glycerin as a moisturizing component.

[0039] Example 1 A method for preparing an antibacterial mouthwash comprises the following steps: (1) Preparation of Lactobacillus plantarum: Lactobacillus plantarum was inoculated into MRS medium, activated and cultured at 37°C for 24 h, pH = 6.0, then the activated Lactobacillus plantarum culture liquid was inoculated into MRS medium at a volume fraction of 5%, fermented and cultured at 40°C for 20 h to obtain Lactobacillus plantarum fermentation liquid, sterilized at 85°C for 20 min, concentrated and dried to obtain Lactobacillus plantarum powder; The main components of MRS medium include: glucose: 20g / L, casein hydrolysate: 10g / L, broth: 10g / L, yeast extract: 5g / L, sodium acetate: 5g / L, polysorbate 80 (Tween80): 80.1g / L, ammonium chloride: 2g / L, dipotassium hydrogen phosphate: 2g / L, magnesium sulfate: 0.5g / L, manganese sulfate: 0.05g / L. After the pH value is adjusted to 6.5, it is sterilized by high-pressure steam at 121°C for 30 minutes.

[0040] (2) Weigh 1 g of sodium benzoate and 1 g of benzoic acid, add them into 30 g of purified water, stir thoroughly to dissolve, then add 3 g of glycerin, 3 g of citric acid, 2 g of menthol, and 10 g of xylitol, stir thoroughly to evenly mix, then weigh 10 g of honeysuckle extract, 10 g of ponzu extract, 20 g of Plectranthus amomum extract, 10 g of tea extract, and 3 g of licorice extract, stir thoroughly to evenly mix to obtain a mixed solution; (3) Add Lactobacillus plantarum powder to the mixed solution at 35°C, mix evenly, and package after passing the test to obtain an antibacterial mouthwash with a long-lasting fragrance.

[0041] By selecting natural herbs such as honeysuckle, hollyhock, Plectranthus, tea, and licorice, and using pure natural herbal essences to reasonably match and extract their effective ingredients, as well as accurately proportioning by weight, the antibacterial effect on multiple pathogens in the oral cavity is achieved, and each also has unique health benefits. The antibacterial mouthwash obtained in this way not only has pure natural herbal properties, but also can keep breath fresh for a long time.

[0042] Example 2 A method for preparing an antibacterial mouthwash comprises the following steps: (1) Preparation of Lactobacillus plantarum: Lactobacillus plantarum was inoculated into MRS medium, activated and cultured at 38°C for 24 h, pH = 7.0, then the activated Lactobacillus plantarum culture liquid was inoculated into MRS medium at a volume fraction of 5%, fermented and cultured at 40°C for 20 h to obtain Lactobacillus plantarum fermentation liquid, sterilized at 85°C for 20 min, concentrated and dried to obtain Lactobacillus plantarum powder; The main components of MRS medium include: glucose: 20g / L, casein hydrolysate: 10g / L, broth: 10g / L, yeast extract: 5g / L, sodium acetate: 5g / L, polysorbate 80 (Tween80): 80.1g / L, ammonium chloride: 2g / L, dipotassium hydrogen phosphate: 2g / L, magnesium sulfate: 0.5g / L, manganese sulfate: 0.05g / L. After the pH value is adjusted to 6.5, it is sterilized by high-pressure steam at 121°C for 30 minutes.

[0043] (2) Weigh 0.8 g of sodium benzoate and 1 g of benzoic acid respectively, add them into 25 g of purified water, stir thoroughly to dissolve, then add 20 g of glycerin, 1 g of citric acid, 1 g of menthol, and 1 g of xylitol respectively, stir thoroughly to evenly mix, then weigh 3 g of honeysuckle extract, 3 g of ponticum odoratum extract, 3 g of pectin extract, 3 g of tea extract, and 3 g of licorice extract, stir thoroughly to evenly mix to obtain a mixed solution; (3) Add Lactobacillus plantarum powder to the mixed solution at 35°C, mix evenly, and package after passing the test to obtain an antibacterial mouthwash that can relieve dryness.

[0044] The antibacterial mouthwash obtained in this way not only has pure natural herbal properties, but also can relieve the phenomenon of dry lips and chapped lips caused by dryness problems.

[0045] Example 3 A method for preparing an antibacterial mouthwash comprises the following steps: (1) Preparation of Lactobacillus plantarum: Lactobacillus plantarum was inoculated into MRS medium, activated and cultured at 40°C for 24 h, pH = 7.0, then the activated Lactobacillus plantarum culture liquid was inoculated into MRS medium at an inoculum volume fraction of 5%, fermented and cultured at 40°C for 20 h to obtain Lactobacillus plantarum fermentation liquid, sterilized at 85°C for 20 min, concentrated and dried to obtain Lactobacillus plantarum powder; The main components of MRS medium include: glucose: 20g / L, casein hydrolysate: 10g / L, broth: 10g / L, yeast extract: 5g / L, sodium acetate: 5g / L, polysorbate 80 (Tween80): 80.1g / L, ammonium chloride: 2g / L, dipotassium hydrogen phosphate: 2g / L, magnesium sulfate: 0.5g / L, manganese sulfate: 0.05g / L. After the pH value is adjusted to 6.5, it is sterilized by high-pressure steam at 121°C for 30 minutes.

[0046] (2) Weigh 0.5 g of sodium benzoate and 1.5 g of benzoic acid respectively, add them into 25 g of purified water, stir thoroughly to dissolve, then add 25 g of glycerin, 1 g of citric acid, 1 g of menthol, and 5 g of xylitol respectively, stir thoroughly to evenly mix, then weigh 5 g of honeysuckle extract, 5 g of ponzu extract, 2 g of Plectranthus amomum extract, 3 g of tea extract, and 3 g of licorice extract, stir thoroughly to evenly mix to obtain a mixed solution; (3) Add Lactobacillus plantarum powder to the mixed solution at 37°C, mix evenly, and package after passing the test to obtain an antibacterial mouthwash that can refresh the mind.

[0047] The antibacterial mouthwash obtained in this way not only has pure natural herbal properties, but also improves the sub-healthy state of mental depression caused by poor rest or excessive work pressure.

[0048] Example 4 A method for preparing an antibacterial mouthwash comprises the following steps: (1) Preparation of Lactobacillus plantarum: Lactobacillus plantarum was inoculated into MRS medium, activated and cultured at 40°C for 24 h, pH value = 6.5, then the activated Lactobacillus plantarum culture liquid was inoculated into MRS medium at an inoculum amount of 5% by volume, fermented and cultured at 40°C for 20 h to obtain Lactobacillus plantarum fermentation liquid, sterilized at 85°C for 20 min, concentrated and dried to obtain Lactobacillus plantarum powder; The main components of MRS medium include: glucose: 20g / L, casein hydrolysate: 10g / L, broth: 10g / L, yeast extract: 5g / L, sodium acetate: 5g / L, polysorbate 80 (Tween80): 80.1g / L, ammonium chloride: 2g / L, dipotassium hydrogen phosphate: 2g / L, magnesium sulfate: 0.5g / L, manganese sulfate: 0.05g / L. After the pH value is adjusted to 6.5, it is sterilized by high-pressure steam at 121°C for 30 minutes.

[0049] (2) Weigh 0.5 g of sodium benzoate and 2.1 g of benzoic acid respectively, add them into 30 g of purified water, stir thoroughly to dissolve, then add 2 g of glycerol, 3 g of citric acid, 2 g of menthol, and 3 g of xylitol respectively, stir thoroughly to obtain a mixed solution, then weigh 5 g of honeysuckle extract, 10 g of ponzu extract, 5 g of Plectranthus amomum extract, 3 g of tea extract, and 2 g of liquorice extract, stir thoroughly to obtain a mixed solution.

[0050] (3) Add Lactobacillus plantarum powder to the mixed solution at 38°C, mix evenly, and package after passing the test to obtain an antibacterial mouthwash that can relieve inflammation.

[0051] The antibacterial mouthwash obtained in this way not only has pure natural herbal properties, but also can relieve the phenomenon of mouth sores caused by inflammation.

[0052] According to the above-mentioned embodiments 1-4 of the present invention, a synergistic effect is produced by combining extracts of honeysuckle, magnolia officinalis, Plectranthus amboinicus, tea leaves, and licorice. Magnolia officinalis extract can dry out dampness and eliminate phlegm, relieve gas and eliminate accumulation, and adjust the lungs and digestive tract; tea polyphenols in tea extract can achieve the effect of preventing caries and strengthening teeth; citric acid has the effect of refreshing breath and cleaning dental plaque. The synergistic effect of these ingredients allows the mouthwash to provide comprehensive oral health protection while being antibacterial.

[0053] Example 5 A method for preparing an antibacterial mouthwash comprises the following steps: (1) Preparation of Lactobacillus plantarum: Lactobacillus plantarum was inoculated into MRS medium, activated and cultured at 37°C for 24 h, pH = 6.0, then the activated Lactobacillus plantarum culture liquid was inoculated into MRS medium at an inoculum amount of 5% by volume, fermented and cultured at 40°C for 20 h to obtain Lactobacillus plantarum fermentation liquid, sterilized at 85°C for 20 min, concentrated and dried to obtain Lactobacillus plantarum powder; The main components of MRS medium include: glucose: 20g / L, casein hydrolysate: 10g / L, broth: 10g / L, yeast extract: 5g / L, sodium acetate: 5g / L, polysorbate 80 (Tween80): 80.1g / L, ammonium chloride: 2g / L, dipotassium hydrogen phosphate: 2g / L, magnesium sulfate: 0.5g / L, manganese sulfate: 0.05g / L. After the pH value is adjusted to 6.5, it is sterilized by high-pressure steam at 121°C for 30 minutes.

[0054] (2) First weigh 3 g of honeysuckle, 6 g of ophiopogon, 9 g of Plectranthus amboinicus, 18 g of tea leaves, and 12 g of licorice, grind and mix them together, reflux them continuously with 2000 g of ethanol for five hours, filter the residue to obtain a reflux solution, and then concentrate to remove excess solvent to obtain a comprehensive extract of honeysuckle, ophiopogon, Plectranthus amboinicus, tea leaves, and licorice. Then weigh 1 g of sodium benzoate and 1 g of benzoic acid respectively, add them into 30 g of purified water, stir well to dissolve, then add 2 g of glycerin, 3 g of citric acid, 2 g of menthol, and 3 g of xylitol respectively, stir well to evenly, then weigh 20 g of the comprehensive extract of honeysuckle, ophiopogon, Plectranthus amboinicus, tea leaves, and licorice, stir well to evenly, and prepare a mixed solution.

[0055] (3) Add Lactobacillus plantarum powder to the mixed solution at 35°C, mix evenly, and package after passing the test to obtain an antibacterial mouthwash that can relieve inflammation.

[0056] The above-mentioned production process of the present invention selects and matches natural herbal extracts, selects natural herbal plants such as honeysuckle, fennel, Plectranthus amomum, tea, and licorice, extracts their effective ingredients and accurately mixes them in weight percentage, and mixes the components evenly to prepare a mouthwash with high antibacterial effect; and uses ethanol reflux method to extract effective ingredients, ensures the extraction efficiency and purity of the extract, and achieves antibacterial effect on multiple pathogens in the oral cavity. These extracts produce synergistic effects, enhance the antibacterial effect, and each of them also has unique health benefits. They not only have pure natural herbal properties, but also can alleviate the phenomenon of oral sores caused by inflammation.

[0057] Performance Testing: Figure 1 This is an antibacterial experiment between the four antibacterial mouthwashes described in the present invention and a control group.

[0058] Purified water was used as a blank control group, a piece of paper stained with purified water was placed in the center of the culture dish, numbered 0, and Examples 1 to 4 were used as experimental groups, and paper pieces stained with antibacterial mouthwash produced according to Examples 1 to 4 were placed around the culture dish, numbered 1 to 4 respectively, for antibacterial experiments. After the culture medium was inoculated with bacteria, it was cultured at a constant temperature of 25°C for 7 days. The antibacterial plaques produced by the antibacterial experiment clearly showed that the purified water in the blank control group had no antibacterial effect at all, and the experimental effects of Examples 1 to 4 were also very obvious, and the antibacterial plaques had been connected into one piece.

[0059] The above comparative experiment was conducted by setting up a blank control group (purified water) and four experimental groups (corresponding to the antibacterial mouthwashes produced in Examples 1 to 4, respectively). The antibacterial effect was observed by placing paper pieces stained with different mouthwashes in a culture dish and inoculating bacteria for constant temperature culture. After 7 days of constant temperature culture, it was observed that no antibacterial plaques were produced around the paper pieces of the blank control group (purified water), indicating that purified water itself had no antibacterial effect. However, obvious antibacterial plaques were produced around the paper pieces of the four experimental groups (i.e., the mouthwashes of Examples 1 to 4), and the antibacterial plaques were connected, indicating that these mouthwashes had significant antibacterial effects.

[0060] Since there are obvious differences between the experimental group and the blank control group, and the experimental groups all show significant antibacterial effects, it can be concluded that the antibacterial mouthwash of the present invention has significant antibacterial effects, which provides a strong scientific basis for its application in the field of oral care.

[0061] Experimental test on the bactericidal performance of the antibacterial mouthwash prepared in Examples 1 to 4: Among them, phosphate buffered saline (hereinafter referred to as PBS) is 0.03 mol / L, which is used to wash the bacterial moss to make the bacterial suspension uniform and then dilute it to the required concentration.

[0062] The neutralizer identification test groups before the bactericidal performance test are: 1) Infected sample + 5mL PBS; 2) Contaminated sample + 5mL neutralizer; 3) Bacteria-stained photo + 5mL neutralizer; 4) Bacterial swatch + 5mL neutralizer + bacterial swatch photo; 5) Bacteria-stained photo + 5mL PBS; 6) PBS from the same batch; 7) Neutralizer from the same batch; 8) Culture medium from the same batch.

[0063] Table 1 Test results of Escherichia coli neutralizer identification test, unit is CFU / mL The neutralizer test data meets the requirements and can be used as the neutralizer identification results of the antibacterial mouthwash prepared by embodiments 1 to 4 of the present invention.

[0064] The 24-hour slant culture of E. coli was washed with PBS to prepare a bacterial suspension, and 100 μL was dropped on the control sample. The number of recovered bacteria was 1x10 4 ~9x10 4 CFU / piece is the concentration. Take 4 pieces of sample pieces of 2.0cmx3.0cm, fully soak the antibacterial mouthwash prepared in Examples 1 to 4, take 1 piece of control sample piece, which does not contain the antibacterial mouthwash prepared in Examples 1 to 4, and sterilize it, and place it in sterilized culture dishes respectively. Take the above bacterial suspension, drip 100μL on each sample piece and control sample piece respectively, evenly apply it, start timing, act for 10min, use sterile tweezers to put the sample pieces into the test tube containing 5mL of the corresponding neutralizer, mix it thoroughly, make appropriate dilutions, then take 2 to 3 dilutions, take 0.5mL respectively and place it in two culture dishes, pour it with 15mL of nutrient agar medium cooled to 40℃~45℃, rotate the culture dish to make it fully uniform, turn the culture dish over after the agar solidifies, and culture it at 35℃±2℃ for 48h to count the viable bacterial colonies.

[0065] The test was repeated 3 times and the sterilization rate was calculated according to the following formula: X = (AB) / Ax100%; X—sterilization rate, %; A—average colony count of control samples; B—Average colony count of the tested samples.

[0066] The sterilization rate is ≥90%, and the product has a sterilization effect.

[0067] According to the test data, the average colony count of the positive control group was 7.8x10 4 There was no bacterial growth in the negative control. Therefore, the test results show that the antibacterial mouthwash described in Examples 1 to 4 can kill Escherichia coli by 99.9% after 10 minutes of use.

[0068] It should be understood that the various forms of processes shown above can be used to reorder, add or delete steps. For example, the steps described in the present invention can be executed in parallel, sequentially or in different orders, as long as the desired results of the technical solution disclosed in the present invention can be achieved, and this document does not limit this.

[0069] The above specific implementations do not constitute a limitation on the protection scope of the present invention. It should be understood by those skilled in the art that various modifications, combinations, sub-combinations and substitutions can be made according to design requirements and other factors. Any modification, equivalent substitution and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An antibacterial mouthwash, characterized in that: The composition is composed of the following raw materials in percentage by weight: 20-30% of plant lactobacillus powder, 25-30% of plant extract, 10-15% of moisturizer, 2-3% of preservative, 0.5-2% of pH adjuster, 5-12% of antibacterial enhancer, 2-3% of sweetener, and 17-35% of deionized water. The plant extracts are honeysuckle extract, magnolia officinalis extract, Plectranthus amboinicus extract, tea extract, and liquorice extract; Lactobacillus plantarum powder is provided by Shanghai Shangjian Testing Technology Co., Ltd., and the product number is BNCC299253.

2. An antibacterial mouthwash according to claim 1, characterized in that: The moisturizer is one or more of glycerol, 1,3-butylene glycol, polyethylene glycol, and sorbitol.

3. An antibacterial mouthwash according to claim 1, characterized in that: The preservative is one or more of sodium benzoate, methylparaben, hydroxybenzoate, and potassium sorbate.

4. An antibacterial mouthwash according to claim 1, characterized in that: The pH adjuster is one or both of citric acid and benzoic acid.

5. An antibacterial mouthwash according to claim 1, characterized in that: The antibacterial synergist is one or more of menthol, thymol and methyl salicylate.

6. An antibacterial mouthwash according to claim 1, characterized in that: The sweetener is one or more of xylitol, sorbitol, D-xylose and thaumatin.

7. A method for preparing an antibacterial mouthwash according to any one of claims 1 to 6, characterized in that: The following steps are involved: (1) inoculating Lactobacillus plantarum into MRS medium or similar medium rich in carbohydrates and nitrogen sources, activating and culturing at 30-40° C. for 24-36 hours, with a pH value of 6.0-7.5, then inoculating the activated Lactobacillus plantarum culture liquid into the MRS medium at an inoculum amount of 5% by volume, fermenting and culturing at 30-40° C. for 20-30 hours to obtain Lactobacillus plantarum fermentation liquid, sterilizing, concentrating and drying to obtain Lactobacillus plantarum powder; (2) The plant extract, moisturizer, preservative, pH adjuster, antibacterial enhancer and sweetener are mixed evenly according to a proportion, and then deionized water is added and stirred to obtain a mixed solution; (3) Add Lactobacillus plantarum powder into the mixed solution at a temperature of 30-40° C. and mix well to prepare an antibacterial mouthwash.

8. The method for preparing an antibacterial mouthwash according to claim 7, characterized in that: In step (1), the similar medium rich in carbohydrates and nitrogen sources is CM0006 medium, and the components of the CM0006 medium are: casein peptone 10.0 g / L, beef extract 10.0 g / L, yeast powder 5.0 g / L, glucose 5.0 g / L, sodium acetate 5.0 g / L, diammonium citrate 2.2 g / L, K2HPO4 1.0 g / L, MgSO4.7H2O 0.4 g / L, MnSO4.H2O 0.05 g / L, CaCO3 2.0 g / L, agar 15 g / L, distilled water 1.0 L, pH 6.8.

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