Chinese herbal medicine composition and application thereof

Abstract

The invention provides a Chinese herbal medicine composition and application thereof. The Chinese herbal medicine composition provided by the invention comprises yellow ginger, origanum vulgare, radix angelicae, gelsmium elegans and asarum. According to the present invention, the components achieve the synergistic effect, such that the prevalence rate of the dactylogyriasis can be inhibited, the tissue damage and the inflammation after the dactylogyriasis infection can be relieved, the survival rate can be improved, and the components are the plant extracts, are easy to degrade, do not easily cause the lasting pollution, and have the environmental protection advantage.

Description

Technical Field

[0001] The invention relates to an additive combination, in particular to a Chinese herbal medicine composition and application thereof, belonging to the field of biotechnology. Background Art

[0002] Dactylogyrus belongs to the Dactylogyridae family, order Dactylogyridae, class Monogenea. It is the genus with the highest species diversity discovered so far. More than 900 species have been found worldwide, and they are distributed in most water systems in China. Various types of Dactylogyrus usually parasitize the gills of the host, stimulating the gills to secrete mucus, affecting breathing, and even destroying the gill tissue, which in turn causes respiratory disorders and infections in the host. The mortality rate is extremely high, which has caused great harm to freshwater fisheries. In traditional aquaculture production, drugs such as trichlorfon, mebendazole, and copper sulfate are usually used to treat Dactylogyrus, but these drugs usually cause great pollution to the environment and have restrictive conditions for use.

[0003] Therefore, it is urgent to develop a technical solution that has little environmental pollution and can effectively prevent and treat dactylariasis. Summary of the invention

[0004] The invention provides a Chinese herbal medicine composition, which has less environmental pollution, can significantly reduce the infection rate of dactylariasis, and effectively prevent and treat dactylariasis.

[0005] The present invention also provides a method for cultivating thick-jawed bream. The Chinese herbal medicine composition is used in the cultivation process of thick-jawed bream to improve the survival rate of thick-jawed bream.

[0006] The present invention provides a Chinese herbal medicine composition on one hand, comprising turmeric, oregano, angelica dahurica, Gelsemium elegans and asarum.

[0007] The Chinese herbal medicine composition as mentioned above comprises, by weight, 3-5 parts of turmeric, 6-8 parts of oregano, 3-5 parts of angelica dahurica, 2-3 parts of Gelsemium elegans, and 2-3 parts of asarum.

[0008] The Chinese herbal medicine composition as described above, wherein the particle size D50 of the Chinese herbal medicine composition is not higher than 300 meshes.

[0009] In another aspect, the present invention provides a method for cultivating thick-jawed bream, wherein the thick-jawed bream is cultivated by placing the above-mentioned Chinese herbal medicine composition into a cultivation facility.

[0010] According to the above-mentioned breeding method, the thick jawed bream is 1-2 years old, and the dosage of the Chinese herbal medicine composition is 2-5g / m 3 ;

[0011] And / or, the thick jawed bream is 3 years old or above, and the amount of the Chinese herbal medicine composition is 80-100 g / m 3 .

[0012] The cultivation method as described above, wherein the thick jawed bream is 1-2 years old, and the cultivation facility comprises algae, and the algae comprises at least one of Chlorella pyrenoidosa, Pinella spp., Scenedesmus spp., Discoideum spp., and Cynomorium spp.;

[0013] Preferably, the density of the algae is 2.5×10 6 cells / ml~5×10 6 cells / ml.

[0014] In the breeding method as described above, the thick-jawed bream is 3 years old or above, the breeding facility also includes sturgeons, and the ratio of the number of the thick-jawed bream to the number of sturgeons is 1:(2-3).

[0015] In the above-mentioned breeding method, the thick jaw bream is 1-2 years old, the breeding facility is a 1-2 mu pond, the breeding water depth is 1-1.5m, and the breeding density is 600-800 tails / m 2 ;

[0016] And / or, the thick jawed bream is 3 years old or above, and the breeding facility is 100-200m 2 Oval cement culture pond, the water depth is 1.5-2m, the stocking density is 2-5 / m 2 .

[0017] The cultivation method as described above, wherein the cultivation facility further comprises eucalyptus essential oil;

[0018] The concentration of eucalyptus essential oil in the culture facility is 2-4 g / m 3 .

[0019] In the cultivation method as described above, the thick jawed bream is 3 years old or above, and the flow rate of the aquaculture water in the cultivation facility is 0.5m / s-0.7m / s.

[0020] The Chinese herbal medicine composition provided by the present invention comprises turmeric, oregano, angelica dahurica, Gelsemium elegans and asarum, wherein turmeric, oregano and angelica dahurica have anti-parasitic and antimicrobial activities and have inhibitory effects on Dactylopidae, while asarum and Gelsemium elegans have certain anti-inflammatory and antioxidant properties. The above components play a synergistic role, which can not only inhibit the morbidity of Dactylopidae disease, but also alleviate tissue damage and inflammation after infection with Dactylopidae, and improve the survival rate. In addition, the above components are all plant extracts, which are easy to degrade, not easy to cause persistent pollution, and have the advantage of being environmentally friendly. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1A comparative bar graph of the plasma complement C3 and C4 contents of thick jawed bream seedlings in the cultivation methods provided in Examples 1 and 5 of the present invention;

[0022] Figure 2 This is a bar chart comparing the antioxidant properties of the liver of thick jawed bream seedlings in the cultivation methods provided in Example 1 and Example 5 of the present invention. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] In the current breeding model, drugs such as trichlorfon, mebendazole, and copper sulfate are usually used to treat dactylitis in thick-jawed bream. However, these drugs will cause pollution to the environment during use.

[0025] From the perspective of environmental friendliness, some Chinese herbal medicine compositions have less pollution to the environment. So how to achieve less environmental pollution and high effect in preventing dactylariasis requires selecting appropriate Chinese herbal medicine compositions.

[0026] The present invention provides a Chinese herbal medicine composition on one hand, comprising turmeric, oregano, angelica dahurica, Gelsemium elegans and asarum.

[0027] The Chinese herbal medicine composition provided by the invention is used for preventing and treating dactylariasis, is environmentally friendly, does not cause a significant impact on the environment, and can significantly reduce the morbidity of dactylariasis and improve the survival rate.

[0028] Among them, turmeric, oregano, and angelica have antiparasitic and antimicrobial activities, and have inhibitory effects on ringworms, while asarum and hook have certain anti-inflammatory and antioxidant properties, which help to reduce tissue damage and inflammatory reactions caused by ringworm infection. The above components play a synergistic role, can enhance immunity, have an inhibitory effect on ringworms, and can also play an anti-inflammatory and antibacterial role, relieve tissue damage and inflammatory symptoms after infection with ringworm disease, reduce the prevalence, and improve the survival rate. Moreover, the various components in the Chinese herbal medicine composition are plant extracts, which are easy to degrade, not easy to cause persistent pollution, and have little impact on the environment.

[0029] The efficacy of Chinese herbal medicine compositions with different mass components is quite different. The inventors have found through a large number of experiments that the Chinese herbal medicine composition includes 3-5 parts of turmeric, 6-8 parts of oregano, 3-5 parts of angelica, 2-3 parts of Gelsemium elegans, and 2-3 parts of Asarum by mass. At this time, the effects of each component can be maximized, providing a variety of biological effects, while effectively controlling the dosage of potentially toxic components, ensuring the safety of the overall Chinese herbal medicine composition, making the Chinese herbal medicine composition have a more beneficial anti-dactylosis effect and improving the survival rate of fish species.

[0030] In a specific embodiment, turmeric, oregano, angelica dahurica, Gelsemium elegans and asarum are weighed according to the above-mentioned mass parts, and then washed, drained and dried. The dried raw materials are stirred, crushed and ground, sieved and sterilized.

[0031] The present invention does not limit the specific method of sterilization, and the common methods in the art may be used for sterilization, such as using an ultraviolet sterilization lamp for sterilization.

[0032] In the process of stirring, crushing and grinding the raw materials, it is necessary to control the particle size of the raw materials within an appropriate range, improve the suspension state of the Chinese herbal composition, increase the feeding opportunities and the degree of entrance of fish, and give full play to the effect of the Chinese herbal composition.

[0033] In one embodiment, the particle size D50 of the Chinese herbal composition is not higher than 300 mesh.

[0034] The particle size of the Chinese herbal composition can be controlled by sieving the raw materials after crushing and grinding.

[0035] In another aspect, the present invention provides a method for cultivating thick-jawed bream, wherein the thick-jawed bream is cultivated by placing the above-mentioned Chinese herbal medicine composition into a cultivation facility.

[0036] Megalobrama pellegrini, commonly known as black bream, belongs to the Cypriniformes, Cyprinidae, Culterinae, Megalobrama genus. It is mainly distributed in the upper reaches of the Yangtze River and the middle and lower reaches of tributaries such as the Wujiang River, Minjiang River, Chishui River, Tuojiang River, and Jialing River. It is one of the fish with high economic value in the upper reaches of the Yangtze River in my country. However, due to the changes in its habitat caused by the construction of water conservancy and hydropower facilities and human overfishing, its wild population has shown a downward trend and its habitat range has continued to shrink. Therefore, Megalobrama pellegrini has also been listed as a third-level protected species. In recent years, many domestic scientific research and breeding units have carried out research on the full artificial breeding technology of Megalobrama pellegrini, but frequent diseases are one of the restrictive technical bottlenecks in the breeding process, especially the ringworm disease, which is the most prominent. The fry can be infected when they grow to more than 5-7cm, covering almost the entire life history process. After being infected with Dactylopii, thick-jawed bream usually exhibits stress behaviors such as rubbing against walls, swimming against currents, and swimming rapidly. Once the above behaviors occur, they are usually accompanied by loss of scales, scratches on fins and skin. Cultivating thick-jawed bream with the above-mentioned Chinese herbal medicine composition can not only inhibit the chance of thick-jawed bream being infected with Dactylopii disease, but also can mitigate the stress behaviors of thick-jawed bream, reduce secondary damage caused by scratches, and improve the survival rate.

[0037] Thick jawed bream can be cultivated by common delivery methods, for example, the Chinese herbal medicine composition can be sprayed into the cultivation facilities.

[0038] The Chinese herbal medicine composition required by thick jaw bream at different growth stages is different. From the perspective of the effect and economy of the Chinese herbal medicine composition, the dosage of the Chinese herbal medicine composition can be adjusted according to different growth stages.

[0039] In a specific embodiment, the thick jaw bream is 1-2 years old, and the amount of the Chinese herbal composition is 2-5g / m 3 .

[0040] The 1-2 years old thick jaw bream in the present invention refers to the stage when the thick jaw bream has not yet reached sexual maturity, and the growth stage of the thick jaw bream can be judged by observing the body shape, scale ring pattern and otolith analysis of the thick jaw bream.

[0041] When the thick jawed bream is 1-2 years old, the body size is relatively small and the intake is low. The dosage of the Chinese herbal composition includes but is not limited to 2 g / m 3 , 2.5g / m 3 , 3g / m 3 3.5g / m 3 , 4g / m 3 4.5g / m 3 , 5g / m 3 Or a range between any two of them.

[0042] In another specific embodiment, the thick jaw bream is 3 years old or older, that is, after the thick jaw bream reaches sexual maturity, the body size and intake of the thick jaw bream are greatly improved, and the amount of the Chinese herbal composition is 80-100g / m 3 In detail, the dosage of the Chinese herbal medicine composition includes but is not limited to 80 g / m 3 , 85g / m 3 , 90g / m 3 , 95g / m 3 , 100g / m 3 Or a range between any two of them.

[0043] Among them, in order to improve the prevention and control effect of dactylariasis, enrofloxacin can be mixed into feed and fed to thick-jawed bream to prevent secondary infection.

[0044] In order to consolidate the cultivation effect of the Chinese herbal composition on thick-jawed bream, the Chinese herbal composition can be added to the cultivation facility again after a certain period of time.

[0045] In a specific embodiment, the Chinese herbal composition is added to the cultivation facility again after an interval of 5 days.

[0046] When the thick-jawed bream is 1-2 years old, the pond area is 1-2 mu. In order to offset the loss of water due to evaporation and control the water quality of the breeding water, water can be injected according to actual conditions, for example, 5% of water can be injected every week.

[0047] It is understandable that in order to ensure the normal growth of thick-jawed bream, the water quality of the aquaculture water needs to be controlled, for example, the dissolved oxygen is controlled at above 8 mg / l, the pH is controlled in the range of 7.5-8.0, dead fish are salvaged daily and the ammonia nitrogen and nitrite contents are tested to ensure that the ammonia nitrogen is < 0.01 mg / L and the nitrite is < 0.1 mg / L.

[0048] Furthermore, in a specific embodiment of the present invention, the thick jaw bream is 1-2 years old, and the cultivation facility includes algae, and the algae include at least one of pyrenoidosa, needle-bar galaxies, scenedesmus, disc star galaxies, and schizontella.

[0049] Chlorella pyrenoidosa can inhibit the ecological effects of Dactylopidae and inhibit their proliferation. In addition, the growth factor CGF in Chlorella pyrenoidosa can improve the ability of cell tissue repair and activate immune cells to improve immunity, which can reduce the damage to infected Dactylopidae seedlings, accelerate the repair of diseased fish gill tissues, and reduce infection mortality. The needle-bar algae, scenedesmus, disc star algae, and round sieve algae can absorb and degrade pollutants in the water, improve water quality, and provide a certain source of nutrition for thick-jawed bream.

[0050] In one embodiment, the density of the algae is 2.5×106 cells / ml~5×10 6 cells / ml, Chlorella pyrenoidosa accounted for more than 65% of the total algae.

[0051] When the density of algae is within the above range, the culture environment of thick jaw bream can be optimized, and the breeding efficiency and fish health level can be improved. This method not only helps the growth of fish, but also promotes sustainable aquaculture practices.

[0052] When the thick jawed bream is 3 years old or older, the flowing water culture mode is adopted. In one specific embodiment, the culture facility area is 100-200m 2 The water change frequency is once a day, the dissolved oxygen is controlled above 6 mg / l, the pH is controlled within the range of 7.0-7.5, dead fish are salvaged daily and the ammonia nitrogen and nitrite contents are tested to ensure that the ammonia nitrogen is <0.01 mg / L and the nitrite is <0.1 mg / L.

[0053] Further, in a specific embodiment, the thick jaw bream is 1-2 years old, the breeding facility is a 1-2 mu pond, the breeding water depth is 1-1.5m, and the breeding density is 600-800 fish / m 2 .

[0054] Specifically, when the thick jaw bream is 1-2 years old, the culture water depth includes but is not limited to 1m, 1.1m, 1.2m, 1.3m, 1.4m, 1.5m or any range between two of them; the culture density includes but is not limited to 600 fish / m 2 、650 / m 2 、700 tails / m 2 、750 / m 2 、800 / m 2 Or a range between any two of them.

[0055] Thick jawed bream is 3 years old and above, and the breeding facilities are 100-200m 2 Oval cement culture pond, the water depth is 1.5-2m, the stocking density is 2-5 / m 2 .

[0056] Specifically, the thick jawed bream must be 3 years old or above, the culture depth includes but is not limited to 1.5m, 1.6m, 1.7m, 1.8m, 1.9m, 2m or any range between them, and the culture density includes but is not limited to 2 fish / m 2 、3 tails / m 2 , 4 tails / m 2 , 5 tails / m 2 Or a range between any two of them.

[0057] Adjusting the aquaculture water depth and stocking density according to the different growth stages of thick-jawed bream can improve growth efficiency and reduce the risk of infection with dactylariasis and other diseases. At the same time, it can optimize resource utilization and improve the immunity and growth rate of thick-jawed bream.

[0058] In a specific embodiment, when the thick-jawed bream is 3 years old or above, the breeding facility also includes sturgeons, and the ratio of the number of thick-jawed bream to sturgeons is 1:(2-3).

[0059] Sturgeons have thick gill filaments and thick mucus, so Dactylorhiza cannot infect sturgeons. Mixing sturgeons and thick-jawed bream can make the thick-jawed bream broodstock more dispersed and less likely to cluster, thereby blocking the transmission of Dactylorhiza between thick-jawed bream and reducing the infection rate.

[0060] The sturgeon of appropriate size can be selected according to the actual size of the breeding facilities and the growth of the thick jaw bream, for example, the body length of the sturgeon is 20-40cm.

[0061] Furthermore, in a specific embodiment, the culture facility also includes eucalyptus essential oil; the concentration of eucalyptus essential oil in the culture facility is 2-4 g / m 3 .

[0062] Specifically, eucalyptus essential oil can be sprayed into the culture facility, and the concentration of eucalyptus essential oil in the culture facility is controlled to include but is not limited to 2 g / m 3 , 2.5g / m 3 , 3g / m 3 3.5g / m 3 , 4g / m 3 Or a range between any two of them.

[0063] In order to increase the dispersion of eucalyptus essential oil in aquaculture water, the eucalyptus essential oil can be diluted first, for example, eucalyptus essential oil can be mixed with water in a mixing ratio of 1:10 and then sprayed.

[0064] It is understandable that in order to ensure the effect of eucalyptus essential oil, if the cultivation mode is a flow cultivation mode, water supply needs to be stopped when spraying eucalyptus essential oil, and water supply should be resumed 3-4 hours after spraying.

[0065] Eucalyptus essential oil has broad-spectrum antibacterial and antifungal properties, which can help control pathogenic microorganisms in water bodies, thereby reducing the risk of secondary infection in fish. Eucalyptus essential oil can also effectively reduce tissue inflammation and damage caused by Dactylopius infection, and has low toxicity to thick-jawed bream at different growth stages, making it green and safe.

[0066] Furthermore, in a specific embodiment of the present invention, the flow rate of aquaculture water in the culture facility is 0.5 m / s-0.7 m / s.

[0067] A high-power flow pump can be used to control the flow rate of aquaculture water in the culture facility. The flow rate of aquaculture water includes but is not limited to 0.5m / s, 0.55m / s, 0.6m / s, 0.65m / s, 0.7m / s or a range between any two of them.

[0068] When the flow rate of the aquaculture water in the culture facility is within the above range, it can not only maintain good water quality, but also flush out the annelids, reduce the stress response of the thick-jawed bream, and improve the growth efficiency and survival rate of the thick-jawed bream.

[0069] The present invention is further described below with reference to embodiments, but the scope of protection claimed by the present invention is not limited to the scope described in the embodiments.

[0070] Example 1

[0071] Thick jawed bream aged 1-2 years were selected for cultivation. The cultivation facility was a large outdoor pond with an area of ​​2 mu. The pond included a density of 4×10 6 cells / ml of algae, of which Chlorella pyrenoidosa accounts for 60% of the algae, the aquaculture depth is controlled at 1.2m, and the aquaculture density is 700 tails / m 2 .

[0072] According to the mass proportion of 2:3:2:1:1, turmeric, oregano, angelica dahurica and Gelsemium elegans are weighed to form a Chinese herbal medicine composition, the particle size D50 of the Chinese herbal medicine composition is not higher than 300 mesh, and the Chinese herbal medicine composition is sprayed into the cultivation facility at a dosage of 3g / m 3 , once a day for 3 consecutive days. At the same time, use enrofloxacin to mix bait before feeding the feed every day, the dosage is 2g of powder per 1kg of feed.

[0073] At the same time, eucalyptus essential oil (Lechang) was diluted with aquaculture water at a ratio of 1:10 and then sprayed into the oxygen finger facility to control the concentration of eucalyptus essential oil to 2g / m 3 , stop the water flow mode during splashing, and resume water supply 3 hours after splashing.

[0074] After 5 days, the gills were examined under a microscope to see if there were any Dactylorhinus residues, and the mortality rate of thick-jawed bream was counted. The details are shown in Table 1.

[0075] Example 2

[0076] Thick jawed bream aged 3 years and above were selected for cultivation. The cultivation facility was an elliptical culture pond with an area of ​​20m 2 The water depth is controlled at 1.5m and the stocking density is 5 / m 2 The flow rate of aquaculture water is controlled at 0.7m / s by a flow pump, and the frequency of water change is 6-8 times / day.

[0077] According to the mass proportion of 2:3:2:1:1, turmeric, oregano, angelica dahurica and Gelsemium elegans are weighed to form a Chinese herbal medicine composition, the particle size D50 of the Chinese herbal medicine composition is not higher than 300 mesh, and the Chinese herbal medicine composition is sprayed into the cultivation facility at a dosage of 80g / m 3 , once a day for 3 consecutive days. At the same time, use enrofloxacin to mix bait before feeding the feed every day, the dosage is 2g of powder per 1kg of feed.

[0078] At the same time, eucalyptus essential oil (Lechang) was diluted with aquaculture water at a ratio of 1:10 and then sprayed into the oxygen finger facility to control the concentration of eucalyptus essential oil to 2g / m 3 , stop the water flow mode during splashing, and resume water supply 4 hours after splashing.

[0079] Sturgeons with a body length of 20-40 cm are released into the breeding pond, and the ratio of thick-jawed bream to sturgeons is 1:1.

[0080] After 5 days, the gills were examined under a microscope to see if there were any Dactylorhinus residues, and the mortality rate of thick-jawed bream was counted. The details are shown in Table 1.

[0081] Example 3

[0082] Thick jawed bream aged 1-2 years were selected for cultivation. The cultivation facility was a large outdoor pond with an area of ​​1 mu The culture pond includes a density of 2.5×10 6 cells / ml of algae, of which Chlorella pyrenoidosa accounts for 75% of the algae, the aquaculture depth is controlled to be 1m, and the aquaculture density is 600 tails / m 2 .

[0083] According to the mass proportion of 1:2:1:1:1, turmeric, oregano, angelica dahurica and Gelsemium elegans are weighed to form a Chinese herbal medicine composition, the particle size D50 of the Chinese herbal medicine composition is not higher than 300 mesh, and the Chinese herbal medicine composition is sprayed into the cultivation facility at a dosage of 2g / m 3 , once a day for 3 consecutive days. At the same time, use enrofloxacin to mix bait before feeding the feed every day, the dosage is 2g of powder per 1kg of feed.

[0084] At the same time, eucalyptus essential oil (Lechang) was diluted with aquaculture water at a ratio of 1:10 and then sprayed into the oxygen finger facility to control the concentration of eucalyptus essential oil to 2g / m 3 , stop the water flow mode during splashing, and resume water supply 3 hours after splashing.

[0085] After 5 days, the gills were examined under a microscope to see if there were any Dactylorhinus residues, and the mortality rate of thick-jawed bream was counted. The details are shown in Table 1.

[0086] Example 4

[0087] Thick-jawed bream aged 3 years and above were selected for cultivation. The cultivation facility was an elliptical culture pond with an area of ​​25m 2The water depth is controlled at 2m and the stocking density is 2 / m 2 The flow rate of aquaculture water is controlled at 0.6m / s by a flow pump, and the frequency of water change is 6-8 times / day.

[0088] A Chinese herbal medicine composition is prepared by weighing turmeric, oregano, angelica dahurica and Gelsemium elegans in a mass ratio of 1:2:1:1:1. The particle size D50 of the Chinese herbal medicine composition is not higher than 300 meshes. The Chinese herbal medicine composition is sprayed into the cultivation facility at a dosage of 100 g / m 3 , once a day for 3 consecutive days. At the same time, use enrofloxacin to mix bait before feeding the feed every day, the dosage is 2g of powder per 1kg of feed.

[0089] At the same time, eucalyptus essential oil (Lechang) was diluted with aquaculture water at a ratio of 1:10 and then sprayed into the oxygen finger facility to control the concentration of eucalyptus essential oil to 2g / m 3 , stop the water flow mode during splashing, and resume water supply 4 hours after splashing.

[0090] Sturgeons with a body length of 20-40 cm are released into the breeding pond, and the ratio of thick-jawed bream to sturgeons is 1:2.

[0091] After 5 days, the gills were examined under a microscope to see if there were any Dactylorhinus residues, and the mortality rate of thick-jawed bream was counted. The details are shown in Table 1.

[0092] Example 5

[0093] In this embodiment, 1-2 years old thick jaw bream is selected for cultivation, and the cultivation method is basically the same as that in Example 1, except that:

[0094] Chlorella pyrenoidosa is not included in the cultivation facilities.

[0095] After 5 days, the gills were examined under a microscope to see if there were any Dactylorhinus residues, and the mortality rate of thick-jawed bream was counted. The details are shown in Table 1.

[0096] Example 6

[0097] In this example, thick jawed bream aged 3 and above were selected for cultivation, and the cultivation method was basically the same as that in Example 2, except that:

[0098] Sturgeons are not stocked in rearing facilities.

[0099] After 5 days, the gills were examined under a microscope to see if there were any Dactylorhinus residues, and the mortality rate of thick-jawed bream was counted. The details are shown in Table 1.

[0100] Example 7

[0101] In this embodiment, 1-2 years old thick jaw bream is selected for cultivation, and the cultivation method is basically the same as that in Example 1, except that:

[0102] Eucalyptus essential oil is not used in cultivation facilities.

[0103] After 5 days, the gills were examined under a microscope to see if there were any Dactylorhinus residues, and the mortality rate of thick-jawed bream was counted. The details are shown in Table 1.

[0104] Example 8

[0105] In this example, thick jawed bream aged 3 and above were selected for cultivation, and the cultivation method was basically the same as that in Example 2, except that:

[0106] The flow rate of aquaculture water in the cultivation facility is controlled at 0.2m / s.

[0107] After 5 days, the gills were examined under a microscope to see if there were any Dactylorhinus residues, and the mortality rate of thick-jawed bream was counted. The details are shown in Table 1.

[0108] Comparative Example 1

[0109] In this comparative example, 1-2 year old thick jaw bream was selected for cultivation, and the cultivation method was basically the same as that in Example 1, except that:

[0110] Trichlorfon (98% purity) and mebendazole (10% solution) were used to replace the Chinese herbal medicine combination, with a trichlorfon dose of 0.5 g / m 3 The dose of mebendazole is 2.5 g / m 3 .

[0111] After 5 days, the gills were examined under a microscope to see if there were any Dactylorhinus residues, and the mortality rate of thick-jawed bream was counted. The details are shown in Table 1.

[0112] Comparative Example 2

[0113] In this comparative example, thick jawed bream aged 3 and above were selected for cultivation, and the cultivation method was basically the same as that in Example 2, except that:

[0114] Trichlorfon was used to replace the Chinese herbal medicine combination, and the dosage of trichlorfon was 80 g / m 3 .

[0115] After 5 days, the gills were examined under a microscope to see if there were any Dactylorhinus residues, and the mortality rate of thick-jawed bream was counted. The details are shown in Table 1.

[0116] Test Case

[0117] Venous blood was drawn from 20 thick jawed bream seedlings in Example 1 and Example 5, and liver tissue was collected. After the blood was centrifuged, the supernatant was taken, and the content of complement C3 and C4 in the plasma was determined by immunoturbidimetry. The liver tissue was ground and crushed to make a homogenate, and the supernatant was taken after centrifugation. The superoxide dismutase (SOD) and catalase (CAT) activities and total antioxidant capacity (T-AOC) in the liver tissue were determined by ultraviolet spectrophotometry.

[0118] in, Figure 1A comparative bar graph of the plasma complement C3 and C4 contents of thick jawed bream seedlings in the cultivation methods provided in Examples 1 and 5 of the present invention; Figure 2 This is a bar chart comparing the antioxidant properties of the liver of thick jawed bream seedlings in the cultivation methods provided in Example 1 and Example 5 of the present invention.

[0119] Table 1

[0120]

[0121] According to Table 1, the application of the technical method of the present invention can reduce the incidence of Dactylopidae in Megalobrama thunbergii. Compared with the traditional drugs for killing Dactylopidae, trichlorfon and mebendazole, the Chinese herbal medicine formula used in the present invention has significant advantages in cure rate and survival rate. Although the morbidity of Examples 5-8 is high, the cure rate and survival rate are higher than the cultivation method provided in Comparative Examples 1-2. Therefore, it can also be considered that the cultivation method provided in Examples 5-8 has greater advantages than the cultivation method provided in Comparative Examples 1-2.

[0122] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese herbal medicine composition, characterized in that: Including turmeric, oregano, angelica dahurica, Gelsemium elegans, and asarum.

2. The Chinese herbal medicine composition according to claim 1, characterized in that: Calculated by weight, it includes 3-5 parts of turmeric, 6-8 parts of oregano, 3-5 parts of angelica dahurica, 2-3 parts of Gelsemium elegans, and 2-3 parts of asarum.

3. The Chinese herbal medicine composition according to claim 1, characterized in that: The particle size D50 of the Chinese herbal medicine composition is not higher than 300 meshes.

4. A method for cultivating thick jaw bream, characterized in that: The Chinese herbal medicine composition according to any one of claims 1 to 3 is put into a cultivation facility to cultivate Megalobrama thick jaws.

5. The cultivation method according to claim 4, characterized in that: The thick jawed bream is 1-2 years old, and the dosage of the Chinese herbal medicine composition is 2-5 g / m 3 ; And / or, the thick jawed bream is 3 years old or above, and the amount of the Chinese herbal medicine composition is 80-100 g / m 3 .

6. The cultivation method according to claim 5, characterized in that: The thick jawed bream is 1-2 years old, and the cultivation facility includes algae, which include at least one of Chlorella pyrenoidosa, Pinella spp., Scenedesmus spp., Discoideum spp., and Cynomorium spp.; Preferably, the density of the algae is 2.5×10 6 cells / ml~5×10 6 cells / ml.

7. The cultivation method according to any one of claims 4 to 6, characterized in that: The thick-jawed bream is 3 years old or older, and the breeding facility also includes sturgeons. The ratio of the number of the thick-jawed bream to the number of sturgeons is 1:(2-3).

8. The cultivation method according to any one of claims 4 to 7, characterized in that: The thick jaw bream is 1-2 years old, the breeding facility is a 1-2 mu pond, the breeding water depth is 1-1.5m, and the breeding density is 600-800 fish / m 2 ; And / or, the thick jawed bream is 3 years old or above, and the breeding facility is 100-200m 2 Oval cement culture pond, the water depth is 1.5-2m, the stocking density is 2-5 / m 2 .

9. The cultivation method according to any one of claims 4 to 8, characterized in that: The cultivation facility also includes eucalyptus essential oil; The concentration of eucalyptus essential oil in the culture facility is 2-4 g / m 3 .

10. The cultivation method according to any one of claims 4 to 9, characterized in that: The thick jaw bream is 3 years old or above, and the flow rate of the aquaculture water in the culture facility is 0.5m / s-0.7m / s.

Images