Chinese herbal medicine composition, preparation method and application of Chinese herbal medicine composition in freckle removing and whitening products

Abstract

The invention discloses a Chinese herbal medicine composition, a preparation method and application of the Chinese herbal medicine composition in freckle-removing and whitening products. The Chinese herbal medicine composition comprises the following raw materials in parts by weight: 45-60 parts of lonicerae flos / dogwood leavening, 20-35 parts of bletilla striata extract and 20-35 parts of curcuma zedoary extract. Wherein the lonicerae flos / dogwood leavening is obtained by activating bifidobacteria, inoculating the bifidobacteria into a seed culture medium, culturing to obtain a seed solution, then transferring the seed solution into a fermentation culture medium, culturing to obtain a fermentation solution, and finally filtering and freeze-drying the fermentation solution; wherein the seed culture medium contains a dogwood extract, and the fermentation culture medium contains a lonicerae flos extract and a dogwood extract. All the components in the Chinese herbal medicine composition have a synergistic effect, and the Chinese herbal medicine composition has the remarkable effects of resisting oxidation, inhibiting tyrosine, repairing ultraviolet aging skin and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese herbal medicines, in particular to a Chinese herbal medicine composition, a preparation method and application thereof in freckle-removing and whitening products. Background Art

[0002] As people's living standards improve, they care more about their image than before. Their awareness and ability to improve their skin problems also increase. The demand for skin care products is increasing, and the safety and effectiveness of skin care products are becoming more and more important. The formation of spots is mostly caused by the accumulation and deposition of melanin, which is produced by human melanocytes and then transported to the surface of human skin to appear as spots. There are currently two methods for removing spots. One is a physical method, which reduces the production of melanin by isolating ultraviolet rays; the second is to inhibit the production of melanin through some active ingredients that can inhibit the activity of tyrosinase.

[0003] Modern whitening and freckle-removing active ingredients are generally obtained through two ways: the first is to obtain extracts of biological agents such as animals and plants through extraction and other methods; the second is to obtain whitening active ingredients through chemical synthesis, such as: glycolic acid, kojic acid and its derivatives, gallic acid, arbutin, vitamin C and its derivatives, glutathione, cysteine, hesperidin, niacinamide, tranexamic acid, salicylic acid, retinoids, ellagic acid, linoleic acid, asiatica glycoside, and glycyrrhizin.

[0004] With the development of Chinese medicine purification technology and the improvement of Chinese medicine preparations, external Chinese medicine has become unique in beauty and skin care products with its small toxic side effects, convenient operation and intuitive efficacy. Our team has combined many years of experience in the research and development of cosmetic formulas and Chinese herbal fermentation technology to research and develop fermentation product raw materials with skin whitening and repair effects, and formulated the raw materials, and finally developed a Chinese herbal medicine composition with whitening, freckle removal, anti-oxidation and skin repair effects. Summary of the invention

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art and to provide a Chinese herbal medicine composition, a preparation method and its application in freckle removal and whitening products to solve the problems raised in the above technical background.

[0006] To achieve the above object, the present invention is implemented through the following technical solutions:

[0007] In a first aspect, the present invention provides a Chinese herbal medicine composition, comprising the following raw materials in parts by weight: 45-60 parts of honeysuckle / cornus officinalis fermentation product, 20-35 parts of bletilla striata extract, and 20-35 parts of zedoaria extract;

[0008] The honeysuckle / cornus officinalis fermented product is obtained by activating bifidobacterium and inoculating it into a seed culture medium to obtain a seed liquid, then transferring the seed liquid to a fermentation medium to obtain a fermentation liquid, and finally filtering and freeze-drying the fermentation liquid; wherein the seed culture medium contains cornus officinalis extract, and the fermentation medium contains honeysuckle extract and cornus officinalis extract.

[0009] Preferably, the Chinese herbal medicine composition comprises the following raw materials in parts by weight: 50 parts of honeysuckle / cornus officinalis fermentation product, 30 parts of bletilla striata extract, and 30 parts of zedoaria extract.

[0010] Preferably, the Bletilla striata extract and the Curcuma zedoaria extract are both obtained by water extraction followed by freeze-drying.

[0011] Preferably, the seed culture medium contains 2.5-4.0 g / L Cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0012] Further preferably, the seed culture medium contains 3.0 g / L Cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0013] Preferably, the fermentation medium contains 0.8-2.5 g / L honeysuckle extract, 1.2-3.0 g / L cornus extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0014] Further preferably, the fermentation medium contains 1.5 g / L honeysuckle extract, 2.5 g / L cornus extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0015] Preferably, the bifidobacterium is Bifidobacterium animalis CICC 6165.

[0016] In a second aspect, the present invention provides a method for preparing the above-mentioned Chinese herbal medicine composition, comprising the following steps:

[0017] S1, activating Bifidobacterium and inoculating it into a seed culture medium containing Cornus officinalis extract and anaerobic culture at 37° C. to obtain seed solution;

[0018] S2, transferring the seed liquid to a fermentation medium containing Lonicera japonica extract and Fructus corni extract and culturing anaerobically at 37° C. to obtain a fermentation liquid;

[0019] S3, filtering and freeze-drying the fermentation liquid to obtain a honeysuckle / cornus officinalis fermentation product;

[0020] S4, extracting with water, concentrating and freeze-drying to obtain Bletilla striata extract and Curcuma zedoaria extract respectively;

[0021] S5. Take 45-60 parts of honeysuckle / Cornus officinalis fermentation product, 20-35 parts of Bletilla striata extract, and 20-35 parts of Curcuma zedoaria extract, and mix them evenly to obtain a Chinese herbal medicine composition.

[0022] In a third aspect, the present invention provides an application of the Chinese herbal medicine composition in freckle removing and whitening products.

[0023] In a fourth aspect, the present invention provides application of the Chinese herbal medicine composition in antioxidant skin repair products.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The present application utilizes a culture medium containing honeysuckle extract and cornus officinalis extract as a raw material, ferments it with animal Bifidobacterium CICC 6165, and compound the fermented honeysuckle / cornus officinalis fermented product with bletilla striata extract and zedoaria extract to obtain a Chinese herbal medicine composition. The components in the Chinese herbal medicine composition act synergistically, have significant antioxidant, tyrosine inhibition and UV-aging skin repair effects, and have broad application prospects in cosmetics and medical beauty products. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 A bar graph showing the effect of the Chinese herbal medicine composition of the present invention on the tyrosinase inhibition rate;

[0027] Figure 2 A bar graph showing the effect of the Chinese herbal composition of the present invention on scavenging rate of DPPH free radicals;

[0028] Figure 3 A bar graph showing the effect of the Chinese herbal medicine composition of the present invention on the SOD activity in the skin tissue of mice with skin photoaging model;

[0029] Figure 4 The bar graph is a graph showing the effect of the Chinese herbal medicine composition of the present invention on the MDA content in the skin tissue of mice with skin photoaging model. DETAILED DESCRIPTION

[0030] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0031] In the present invention, honeysuckle, cornus officinalis, bletilla striata and zedoaria are all purchased from the market, and the quality requirements all meet the standards of the 2015 edition of the Pharmacopoeia.

[0032] In the present invention, the honeysuckle extract, cornus officinalis extract, bletilla striata extract and zedoaria extract are all prepared by water extraction followed by concentration and freeze-drying; specifically:

[0033] Preparation of honeysuckle extract: crush the honeysuckle into 100 mesh particles, mix with 10 times the weight of water, extract by ultrasonic in a 45°C water bath for 2 hours, centrifuge and filter the extract, and set the filtrate aside; take the residue and continue to mix with 10 times the weight of water, boil for 1.5 hours, filter, combine the two filtrates and concentrate and freeze-dry to obtain the honeysuckle extract.

[0034] Preparation of Cornus officinalis extract: Grind Cornus officinalis into 100 mesh particles, mix with 10 times the weight of water, and extract by ultrasonic in a water bath at 45°C for 2 hours. Centrifuge and filter the extract, and use the filtrate for later use; take the residue and continue to mix with 10 times the weight of water, boil for 1.5 hours, filter, combine the two filtrates and concentrate and freeze-dry to obtain the Cornus officinalis extract.

[0035] Preparation of Bletilla striata extract: Take Bletilla striata, crush it into 100 mesh particles, add 10 times the amount of water, decoct for 1 hour, filter, and set aside the filtrate; take the residue and continue to add 8 times the amount of water, decoct for 40 minutes, filter, and set aside the filtrate; take the residue and continue to add 8 times the amount of water, decoct for 40 minutes, filter, discard the residue, combine the filtrates for three times and concentrate and freeze-dry to obtain the Bletilla striata extract.

[0036] Preparation of Curcuma zedoaria extract: Take Curcuma zedoaria, crush it into 100 mesh particles, add 10 times the amount of water, decoct for 1 hour, filter, and set aside the filtrate; take the residue and continue to add 8 times the amount of water, decoct for 40 minutes, filter, and set aside the filtrate; take the residue and continue to add 8 times the amount of water, decoct for 40 minutes, filter, discard the residue, combine the filtrates for three times and concentrate and freeze-dry to obtain the Curcuma zedoaria extract.

[0037] Honeysuckle / Cornus officinalis ferment:

[0038] (1)Strain

[0039] Bifidobacterium animalis CICC 6165 was purchased from China Industrial Microbiological Culture Collection Center.

[0040] (2) Preparation method:

[0041] S1. Use a sterile pipette to draw 0.5 ml of liquid culture medium into the freeze-dried tube to completely dissolve the freeze-dried bacterial powder of animal Bifidobacterium CICC 6165, transfer the dissolved bacterial suspension into a test tube containing 5 ml of liquid culture medium, mix well, and place in an anaerobic incubator at 37°C for 72 hours. Spread the activated liquid after 72 hours of culture on the solid culture medium, culture it anaerobically at 37°C, and after a single colony grows, take a single colony, dilute it with 0.5 mL of sterile water, and inoculate it into the seed culture medium at a volume ratio of 3 v / v%, and culture it anaerobically at 37°C for 24 hours to obtain the seed liquid;

[0042] Among them, the solid culture medium: 10.0g / L beef extract, 5.0g / L peptone, 3.0g / L yeast extract, 5.0g / L glucose, 1.0g / L soluble starch, 5.0g / L NaCl, 3.0g / L NaAc, 0.5g / L L-cysteine ​​hydrochloride, 15g / L agar, adjust the pH to 6.8, sterilize at 121°C for 15min, and set aside. The liquid culture medium does not add agar. The seed culture medium contains 2.5-4.0g / L Cornus officinalis extract, 10.0g / L beef extract, 5.0g / L peptone, 3.0g / L yeast extract, 5.0g / L glucose, 1.0g / L soluble starch, 5.0g / L NaCl, 3.0g / L NaAc, 0.5g / L L-cysteine ​​hydrochloride, adjust the pH to 6.8, sterilize at 121°C for 15min, and set aside.

[0043] S2. Transfer the seed liquid into a fermentation medium at 3v / v%, and culture it anaerobically at 37°C for 48h to obtain a fermentation liquid; wherein the fermentation medium contains 0.8-2.5g / L honeysuckle extract, 1.2-3.0g / L cornus extract, 10.0g / L beef extract powder, 5.0g / L peptone, 3.0g / L yeast extract powder, 5.0g / L glucose, 1.0g / L soluble starch, 5.0g / LNaCl, 3.0g / LNaAc, 0.5g / L L-cysteine ​​hydrochloride, adjust the pH to 6.8, sterilize at 121°C for 15min, and set aside.

[0044] S3, the fermentation liquid was treated at 121°C for 15 minutes to inactivate the fermentation liquid, 0.5m / v% coconut shell activated carbon (10-18 mesh, Henan Jingke Water Treatment Materials Co., Ltd.) was added to the inactivated fermentation liquid, and after standing overnight, the supernatant was filtered out, concentrated under reduced pressure and freeze-dried to obtain the honeysuckle / dogwood fermentation product. 0.5m / v% means that 0.5g coconut shell activated carbon was added to 100ml fermentation liquid.

[0045] Example 1

[0046] This embodiment provides a Chinese herbal medicine composition, and the preparation method thereof comprises the following steps:

[0047] S1. After being activated, Bifidobacterium animalis CICC 6165 was inoculated into a seed culture medium containing Cornus officinalis extract and cultured anaerobically at 37° C. to obtain a seed solution. Bifidobacterium animalis CICC 6165 was purchased from China Industrial Microbiological Culture Collection Center.

[0048] S2. Transfer the seed liquid to a fermentation medium containing honeysuckle extract and cornus extract and culture it anaerobically at 37°C to obtain a fermentation liquid; wherein the seed culture medium contains 2.5g / L cornus extract, 10.0g / L beef extract powder, 5.0g / L peptone, 3.0g / L yeast extract powder, 5.0g / L glucose, 1.0g / L soluble starch, 5.0g / L NaCl, 3.0g / L NaAc, and 0.5g / L L-cysteine ​​hydrochloride.

[0049] S3, filtering and freeze-drying the fermentation liquid to obtain a honeysuckle / cornus officinalis fermentation product; wherein the fermentation medium comprises 2.5 g / L honeysuckle extract, 1.2 g / L cornus officinalis extract, 10.0 g / L beef extract, 5.0 g / L peptone, 3.0 g / L yeast extract, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride. The specific preparation method of the honeysuckle / cornus officinalis fermentation product is shown in the above description.

[0050] S4, extracting with water, concentrating and freeze-drying to obtain Bletilla striata extract and Curcuma zedoaria extract, respectively. The specific preparation methods are described above;

[0051] S5. Take 45 parts of honeysuckle / Cornus officinalis fermented product, 35 parts of Bletilla striata extract, and 35 parts of Curcuma zedoaria extract, mix them evenly to obtain a Chinese herbal medicine composition.

[0052] Example 2

[0053] This embodiment provides a Chinese herbal medicine composition, and the preparation method thereof comprises the following steps:

[0054] S1. After being activated, Bifidobacterium animalis CICC 6165 was inoculated into a seed culture medium containing Cornus officinalis extract and cultured anaerobically at 37° C. to obtain a seed solution. Bifidobacterium animalis CICC 6165 was purchased from China Industrial Microbiological Culture Collection Center.

[0055] S2. Transfer the seed liquid to a fermentation medium containing honeysuckle extract and cornus extract and culture it anaerobically at 37°C to obtain a fermentation liquid; wherein the seed culture medium contains 4.0 g / L cornus extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0056] S3, filtering and freeze-drying the fermentation liquid to obtain a honeysuckle / cornus officinalis fermentation product; wherein the fermentation medium comprises 0.8 g / L honeysuckle extract, 3.0 g / L cornus officinalis extract, 10.0 g / L beef extract, 5.0 g / L peptone, 3.0 g / L yeast extract, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride. The specific preparation method of the honeysuckle / cornus officinalis fermentation product is shown in the above description.

[0057] S4, extracting with water, concentrating and freeze-drying to obtain Bletilla striata extract and Curcuma zedoaria extract, respectively. The specific preparation methods are described above;

[0058] S5. Take 60 parts of honeysuckle / Cornus officinalis fermented product, 20 parts of Bletilla striata extract, and 20 parts of Curcuma zedoaria extract, and mix them evenly to obtain a Chinese herbal medicine composition.

[0059] Example 3

[0060] This embodiment provides a Chinese herbal medicine composition, and the preparation method thereof comprises the following steps:

[0061] S1. After being activated, Bifidobacterium animalis CICC 6165 was inoculated into a seed culture medium containing Cornus officinalis extract and cultured anaerobically at 37° C. to obtain a seed solution. Bifidobacterium animalis CICC 6165 was purchased from China Industrial Microbiological Culture Collection Center.

[0062] S2. Transfer the seed liquid to a fermentation medium containing honeysuckle extract and cornus extract and culture it anaerobically at 37°C to obtain a fermentation liquid; wherein the seed culture medium contains 3.0 g / L cornus extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0063] S3, filtering and freeze-drying the fermentation liquid to obtain a honeysuckle / cornus officinalis fermentation product; wherein the fermentation medium comprises 1.5 g / L honeysuckle extract, 2.5 g / L cornus officinalis extract, 10.0 g / L beef extract, 5.0 g / L peptone, 3.0 g / L yeast extract, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride. The specific preparation method of the honeysuckle / cornus officinalis fermentation product is shown in the above description.

[0064] S4, extracting with water, concentrating and freeze-drying to obtain Bletilla striata extract and Curcuma zedoaria extract, respectively. The specific preparation methods are described above;

[0065] S5. Take 50 parts of honeysuckle / Cornus officinalis fermented material, 30 parts of Bletilla striata extract, and 30 parts of Curcuma zedoaria extract, and mix them evenly to obtain a Chinese herbal medicine composition.

[0066] Comparative Example 1

[0067] This embodiment is similar to embodiment 3, except that the Lonicera japonica / Cornus officinalis fermentation product is removed.

[0068] Comparative Example 2

[0069] This embodiment is similar to embodiment 3, except that the Bletilla striata extract is removed.

[0070] Comparative Example 3

[0071] This embodiment is similar to embodiment 3, except that the Curcuma zedoaria extract is removed.

[0072] Comparative Example 4

[0073] This embodiment is similar to embodiment 3, and the Chinese herbal medicine formula is the same, except that: the formula is different in the fermentation of honeysuckle / cornus officinalis, and the seed culture medium used for the fermentation of honeysuckle / cornus officinalis is different, and the seed culture medium contains 10.0g / L beef extract powder, 5.0g / L peptone, 3.0g / L yeast extract powder, 5.0g / L glucose, 1.0g / L soluble starch, 5.0g / L NaCl, 3.0g / L NaAc, and 0.5g / L L-cysteine ​​hydrochloride. The fermentation medium is the same as that of embodiment 3;

[0074] Comparative Example 5

[0075] This embodiment is similar to embodiment 3, and the Chinese herbal medicine formula is the same, except that: the formula is different in the honeysuckle / cornus officinalis fermentation product, and the fermentation medium used for the honeysuckle / cornus officinalis fermentation product is different, and the fermentation medium contains 1.5g / L honeysuckle extract, 10.0g / L beef extract powder, 5.0g / L peptone, 3.0g / L yeast extract powder, 5.0g / L glucose, 1.0g / L soluble starch, 5.0g / L NaCl, 3.0g / L NaAc, and 0.5g / L L-cysteine ​​hydrochloride. The seed culture medium is the same as that of embodiment 3;

[0076] Comparative Example 6

[0077] This embodiment is similar to embodiment 3, and the Chinese herbal medicine formula is the same, except that: the formula is different in the fermentation of honeysuckle / cornus officinalis, and the fermentation medium used for the fermentation of honeysuckle / cornus officinalis is different, and the fermentation medium contains 2.5g / L cornus officinalis extract, 10.0g / L beef extract powder, 5.0g / L peptone, 3.0g / L yeast extract powder, 5.0g / L glucose, 1.0g / L soluble starch, 5.0g / L NaCl, 3.0g / L NaAc, and 0.5g / L L-cysteine ​​hydrochloride. The seed culture medium is the same as that of embodiment 3;

[0078] Comparative Example 7

[0079] This embodiment is similar to embodiment 3, and the Chinese herbal medicine formula is the same, except that: the formula is different in the honeysuckle / cornus officinalis fermentation product, and the seed culture medium and fermentation medium used in the honeysuckle / cornus officinalis fermentation product are different, and the seed culture medium contains 10.0g / L beef extract powder, 5.0g / L peptone, 3.0g / L yeast extract powder, 5.0g / L glucose, 1.0g / L soluble starch, 5.0g / L NaCl, 3.0g / L NaAc, and 0.5g / L L-cysteine ​​hydrochloride. The fermentation medium contains 10.0g / L beef extract powder, 5.0g / L peptone, 3.0g / L yeast extract powder, 5.0g / L glucose, 1.0g / L soluble starch, 5.0g / L NaCl, 3.0g / L NaAc, and 0.5g / L L-cysteine ​​hydrochloride.

[0080] Performance test of Chinese herbal medicine composition:

[0081] 1. Tyrosinase inhibition test

[0082] 1.1 Reagents: pH 6.8 sodium hydrogen phosphate-citric acid buffer solution: 0.2 mol / L Na2HPO4·12H2O 154.5 mL, add 0.1 mol / L citric acid monohydrate 45.5 mL, and prepare 200 mL of sodium hydrogen phosphate-citric acid buffer solution; use the pH 6.8 sodium hydrogen phosphate-citric acid buffer solution to prepare 100 U / mL tyrosinase solution and 1 mg / mL levodopa solution;

[0083] 1.2 Sample solution: After dissolving the Chinese herbal medicine compositions obtained in Examples 1-3 and Comparative Examples 1-7 using pH 6.8 disodium hydrogen phosphate-citric acid buffer, dilute to a corresponding sample concentration of 0.2 wt% (mass percentage concentration). The solution containing 0.09% of the fermented product of Lonicera japonica / Cornus officinalis was prepared using pH 6.8 disodium hydrogen phosphate-citric acid buffer, the solution containing 0.05% of the extract of Bletilla striata was prepared using pH 6.8 disodium hydrogen phosphate-citric acid buffer, and the solution containing 0.05% of the extract of Curcuma zedoaria was prepared using pH 6.8 disodium hydrogen phosphate-citric acid buffer.

[0084] 1.3 Construct a tyrosinase catalytic reaction system to evaluate the inhibitory effects of the Chinese herbal medicine compositions obtained in Examples 1 to 3 and Comparative Examples 1-7, the fermented products of Honeysuckle / Cornus officinalis, Bletilla striata extract, and Curcuma zedoaria extract on tyrosinase. Referring to the tyrosinase catalytic reaction system (Table 1), four reaction tubes A1, A2, A3, and A4 were divided for sample addition. The sample solution, pH 6.8 disodium hydrogen phosphate-citrate buffer, and tyrosinase solution were respectively drawn into the reaction tubes, shaken well, and incubated in a 37°C water bath for 10 minutes. After taking out, levodopa solution was added. After reacting for 5 minutes, the absorbance values ​​of A1, A2, A3, and A4 were measured at 475nm. Each sample was repeated 3 times. The tyrosinase inhibition rate was calculated according to the following formula. The results are shown in Tables 2 and Figure 1 shown.

[0085] Tyrosinase inhibition rate (%) = [1-(A3-A4) / (A1-A2)] × 100%, where A1, A2, A3, and A4 are the absorbance values ​​at 475 nm after the reaction in the corresponding reaction tube is completed.

[0086] Table 1

[0087]

[0088] 2. DPPH free radical scavenging test

[0089] 2.1 Sample solution: After dissolving the Chinese herbal medicine compositions obtained in Examples 1-3 and Comparative Examples 1-7 with distilled water, dilute to a corresponding sample concentration of 0.2 wt% (mass percentage concentration). Distilled water was used to prepare a solution containing 0.09% of the fermented product of Lonicera japonica / Cornus officinalis, distilled water was used to prepare a solution containing 0.05% of the extract of Bletilla striata, and distilled water was used to prepare a solution containing 0.05% of the extract of Bletilla striata. The extract of Curcuma zedoaria was used to prepare a solution containing 0.05% of the extract of Curcuma zedoaria.

[0090] 2.2 Take 1mL of sample solution, add 1mL of 0.2mmol / L DPPH ethanol solution, mix well and react in the dark at room temperature for 30min, then use a microplate reader to measure the absorbance of the sample at 517nm; the background group is a mixture of 1mL sample solution and 1mL anhydrous ethanol, and the blank control group is a mixture of 1mL distilled water and 1mL DPPH anhydrous ethanol solution. The DPPH free radical scavenging rate is calculated according to the formula, and the results are shown in Table 2 and Figure 2 As shown. The calculation formula is: Q = [1-(A1-A2) / A0] × 100%,

[0091] Where:

[0092] Q: DPPH free radical scavenging rate, %;

[0093] A1: absorbance value of sample group;

[0094] A2: absorbance value of background group;

[0095] A0: absorbance of blank control group.

[0096] Table 2

[0097]

[0098] From Table 2, Figure 1 and Figure 2 It can be seen that the tyrosinase inhibition rate and DPPH free radical scavenging rate of the Chinese herbal composition provided in Examples 1 to 3 are significantly higher than those of the Chinese herbal composition, honeysuckle / Cornus officinalis fermentation product, bletilla striata extract, and curcuma zedoaria extract provided in Comparative Examples 1 to 7, indicating that the Chinese herbal composition provided in the examples of the present application has better antioxidant capacity and stronger tyrosinase inhibition rate; it indicates that the honeysuckle / Cornus officinalis fermentation product, bletilla striata extract, and curcuma zedoaria extract in the Chinese herbal composition have a synergistic effect and have significant whitening and antioxidant effects.

[0099] 3. Effect of Chinese herbal medicine combination on repairing skin photoaging

[0100] 3.1 Animal grouping and drug administration

[0101] 100 female KM mice were purchased from Beijing Huafukang Biotechnology Co., Ltd.; after normal feeding for one week, they were randomly divided into 10 groups, namely a normal group, a model group, and 8 drug-treated groups, with 10 mice in each group. The drug-treated groups were respectively treated with the corresponding Chinese medicine compositions obtained in Example 3 and Comparative Examples 1-7, and the Chinese medicine compositions were diluted with physiological saline to a 0.2% solution.

[0102] Normal group: During the experimental period, the mice were depilated with a shaver at the center of their backs every day, with a depilatory area of ​​2 cm × 3 cm, without UV radiation and drug treatment;

[0103] Model group: During the experimental period, the mice were shaved with a shaver at the center of their backs every day, with a 2 cm × 3 cm hair removal area, and UV radiation + no drug treatment;

[0104] Dosing group: During the experimental period, the mice were depilated with a shaver at the center of their backs every day, with a depilatory area of ​​2 cm×3 cm, and the mice were treated with the corresponding Chinese medicine composition obtained in Example 3 and Comparative Examples 1-7 (2 mg each time) 1 hour before UV radiation + UV radiation;

[0105] 2. Construction of skin photoaging model mice

[0106] The mouse back center was depilated with a shaver, and the depilated area was 2cm×3cm. Three UVA and five UVB lamps were suspended 30cm above the exposed skin on the back of the rats at intervals of 5cm. The experiment lasted for 10 weeks. The radiation dose of UVA combined with UVB was 1 MED (100mJ / cm 2 ) for irradiation, 4 times a week (Monday, Wednesday, Friday, Sunday), and the irradiation dose increases by one MED each week compared with the previous week until 4 MEDs in the fourth week. The irradiation dose is maintained at 4 MEDs in the fourth week and thereafter.

[0107] The normal group was depilated every day, without ultraviolet radiation and drug treatment; the model group was depilated every day, ultraviolet radiation was performed on Monday, Wednesday, Friday and Sunday (the radiation dose is shown in the above description), and no drug treatment was performed; the drug administration group: depilated every day, ultraviolet radiation was performed (the radiation dose is shown in the above description), and the corresponding traditional Chinese medicine composition obtained in Example 3 and Comparative Examples 1-7 was applied 1 hour before ultraviolet radiation (2 mg each time);

[0108] 4. Test indicators

[0109] After the experiment, the mice in each group were killed by dislocation of the neck, and the skin tissues on the backs of the mice in each group were taken. After the skin tissues were peeled off from the subcutaneous fat, they were rinsed three times with pre-cooled saline and dried with filter paper, weighed, and the tissues were cut into pieces under ice bath conditions. Nine times the amount of pre-cooled 0.9% saline was added, and the tissues were homogenized and centrifuged under ice bath conditions. The SOD activity and MDA content were detected using a kit (Nanjing Jiancheng Bioengineering Institute). The results are shown in Table 3. Figure 3 and Figure 4 shown.

[0110] Table 3

[0111]

[0112]

[0113] From Table 3, Figure 3 and Figure 4 It can be seen that compared with the normal group, the SOD activity in the skin tissue of the model group mice was significantly reduced, while the MDA content was significantly increased, indicating that the modeling was successful and the mouse skin was photoaged. Compared with comparative examples 1-7, the SOD activity of the mouse skin tissue of Example 3 was significantly increased, and its MDA content was significantly reduced; this shows that the synergistic effect of honeysuckle / cornus officinalis fermentation, Bletilla striata extract, and Curcuma zedoaria extract can repair the SOD activity in the skin tissue of photoaged rats, reduce the MDA content, and have the effect of repairing UV-aged skin.

[0114] This shows that the Chinese herbal medicine composition provided in the present application can not only whiten and remove spots, and resist oxidation, but also repair the photo-aged skin after ultraviolet irradiation.

[0115] The above-mentioned embodiments only express the specific implementation of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A Chinese herbal medicine composition, characterized in that: The invention comprises the following raw materials in parts by weight: 45-60 parts of honeysuckle / cornus officinalis fermented material, 20-35 parts of bletilla striata extract, and 20-35 parts of zedoaria extract; The honeysuckle / cornus officinalis fermented product is obtained by activating bifidobacterium and inoculating it into a seed culture medium to obtain a seed liquid, then transferring the seed liquid to a fermentation medium to obtain a fermentation liquid, and finally filtering and freeze-drying the fermentation liquid; wherein the seed culture medium contains cornus officinalis extract, and the fermentation medium contains honeysuckle extract and cornus officinalis extract.

2. A Chinese herbal medicine composition according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 50 parts of honeysuckle / cornus officinalis fermented material, 30 parts of bletilla striata extract and 30 parts of zedoaria extract.

3. A Chinese herbal medicine composition according to claim 1, characterized in that: The bletilla striata extract and the zedoary turmeric extract are both obtained by water extraction followed by freeze-drying.

4. A Chinese herbal medicine composition according to claim 1, characterized in that: The seed culture medium comprises 2.5-4.0 g / L of cornus officinalis extract, 10.0 g / L of beef extract powder, 5.0 g / L of peptone, 3.0 g / L of yeast extract powder, 5.0 g / L of glucose, 1.0 g / L of soluble starch, 5.0 g / L of NaCl, 3.0 g / L of NaAc, and 0.5 g / L of L-cysteine ​​hydrochloride.

5. A Chinese herbal medicine composition according to claim 4, characterized in that: The fermentation medium comprises 0.8-2.5 g / L honeysuckle extract, 1.2-3.0 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

6. A Chinese herbal medicine composition according to claim 5, characterized in that: The seed culture medium comprises 3.0 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride; The fermentation medium comprises 1.5 g / L honeysuckle extract, 2.5 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

7. A Chinese herbal medicine composition according to claim 1, characterized in that: The bifidobacterium is selected from Bifidobacterium animalis CICC 6165.

8. The method for preparing the Chinese herbal medicine composition according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1, activating Bifidobacterium and inoculating it into a seed culture medium containing Cornus officinalis extract and anaerobic culture at 37° C. to obtain seed solution; S2, transferring the seed liquid to a fermentation medium containing Lonicera japonica extract and Fructus corni extract and culturing anaerobically at 37° C. to obtain a fermentation liquid; S3, filtering and freeze-drying the fermentation liquid to obtain a honeysuckle / cornus officinalis fermentation product; S4, extracting with water, concentrating and freeze-drying to obtain Bletilla striata extract and Curcuma zedoaria extract respectively; S5. Take 45-60 parts of honeysuckle / Cornus officinalis fermentation product, 20-35 parts of Bletilla striata extract, and 20-35 parts of Curcuma zedoaria extract, and mix them evenly to obtain a Chinese herbal medicine composition.

9. Use of the Chinese herbal medicine composition according to any one of claims 1 to 7 in freckle-removing and whitening products.

10. Use of the Chinese herbal medicine composition according to any one of claims 1 to 7 in antioxidant skin repair products.

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