Preparation device and preparation method of sodium alginate gel microspheres

Abstract

The invention relates to the field of biological materials, and discloses a preparation device and a preparation method of sodium alginate gel microspheres. The preparation device comprises an injection pump, an electric fan and an injector, the injector and the electric fan are arranged on the top of the injection pump, and a push rod of the injector is connected with the injection pump. The preparation method comprises the following steps: preparing materials for preparing sodium alginate gel microspheres, including a sodium alginate solution and a calcium chloride solution; adding the sodium alginate solution into an injector; setting the extrusion rate of an injection pump and the air speed of an electric fan; and starting the injection pump and the electric fan, enabling the sodium alginate solution to be stably extruded through the needle tip, then to be separated from the needle tip and enter the calcium chloride solution, and obtaining the sodium alginate gel microspheres after the cross-linking reaction is completed. According to the method, the wind power is utilized to drive the sodium alginate liquid drops to be quickly separated from the extrusion needle tip of the microfluidic equipment, and the sodium alginate liquid drops enter the calcium chloride solution to be crosslinked and balled, so that the efficient and accurate preparation of the sodium alginate gel microspheres is realized.

Description

Technical Field

[0001] The present invention relates to the field of biomaterials, and more particularly to a preparation device and a preparation method of sodium alginate gel microspheres. Background Art

[0002] At present, the commonly used methods for batch preparation of sodium alginate gel spheres mainly include ionic crosslinking, electrostatic spraying and microfluidics. Among them, the ionic crosslinking method for preparing sodium alginate gel spheres is a preparation method based on the principle of ionic crosslinking. In this method, the sodium alginate solution reacts with multivalent cations to form a crosslinked structure, and finally forms a coagulated spherical structure. Patent CN202111190093.5 uses the physical and chemical effects between polyamide-amine and heavy metal ions to further improve the adsorption and removal ability of sodium alginate gel spheres for heavy metal ions. However, in this patent, during the ionic crosslinking process of sodium alginate, too much or too little crosslinking agent may cause the gel structure to be unstable or form uneven gel spheres, making it difficult to achieve precise control of the morphology and uniformity of the sodium alginate gel spheres. Another method for preparing sodium alginate gel spheres by electrostatic spraying is to spray the sodium alginate solution into small droplets through a high-voltage electrostatic field, and then form gel spheres under the action of an ionic crosslinking agent. However, this preparation method requires specialized equipment and conditions, including a high-voltage electrostatic spray system, which increases the cost and complexity of preparing sodium alginate gel spheres. Patent CN201910652544.9 uses a coaxial electrostatic spray method to prepare sodium alginate gel spheres. Although the prepared sodium alginate gel spheres have a good spherical structure and controllable particle size, the sodium alginate gel spheres prepared by electrospraying are smaller in size and are more suitable for the preparation of micro- and nano-sized sodium alginate gel spheres.

[0003] In addition, the method of preparing sodium alginate gel spheres based on microfluidic technology is to precisely control the mixing and reaction of fluids through microchannels and microvalves inside the microfluidic chip to form gel spheres. For example, Satoru Yoshida et al. used a new type of microfluidic technology to use alginate hydrogel as a "sacrificial gel template" to maintain the position of extracellular matrix molecules in the gel spheres, and prepared anisotropic hydrogel microparticles with different types of extracellular matrix. Patent CN202311117962.0 uses droplet microfluidics technology to mix equal volumes of gel ion solution, gel ion chelating agent and sodium alginate solution, and then mix it with equal volumes of exchange ion solution, exchange ion chelating agent and sodium alginate solution. After cross-linking reaction and curing under the action of oil phase, alginate gel microspheres are obtained. However, this technology has the disadvantages of high cost and complex operation in the process of preparing sodium alginate gel spheres, and the sodium alginate gel spheres are easily contaminated by the oil phase and prone to pore blockage. In addition, Jing-Hao Cui et al. used a spray method to prepare sodium alginate microspheres. After characterizing the size and surface morphology of the gel balls, they found that the average particle size of the prepared microspheres increased with the increase of sodium alginate concentration and delivery rate, and decreased with the decrease of air pressure. The ratio of the delivery rate of sodium alginate solution to the air pressure will affect the size and shape of the sodium alginate particles.

[0004] Although the above preparation method has certain feasibility, it still has deficiencies in the process of preparing sodium alginate gel spheres with a particle size of 100 to 1000 microns in batches. For example, in the ionic gelation preparation process, due to differences in the solubility, stirring speed, temperature, pH value, etc. of sodium alginate and the cross-linking agent, it is easy to cause uneven mixing of the solution, thereby affecting the uniformity of the gel spheres. In addition, the spray drying method requires special electrospray equipment, and the sodium alginate gel spheres formed by spray drying have a small particle size and cannot meet the demand for preparing sodium alginate gel spheres with a particle size of 100 to 1000 microns. Although microfluidic technology can efficiently prepare high-quality sodium alginate gel spheres, it needs to use a dedicated microfluidic chip channel. In addition, it needs to form oil / water micro droplets by adding an oil phase, which is more complicated, more expensive, and easily causes the sample to be contaminated by the oil phase. Summary of the Invention

[0005] The present invention aims to overcome the defects of the gel spheres described in the prior art, such as the lack of consistency in particle size and morphology, expensive preparation equipment and complicated operation, and provides a preparation device for sodium alginate gel microspheres.

[0006] Another object of the present invention is to provide a method for preparing sodium alginate gel microspheres.

[0007] In order to solve the above technical problems, the technical solutions of the present invention are as follows:

[0008] A device for preparing sodium alginate gel microspheres comprises: a syringe pump, a syringe, and an electric blower; the syringe and the electric blower are placed on top of the syringe pump, a syringe push rod is connected to the syringe pump, and the syringe pump drives the push rod to move; the air outlet of the electric blower faces the needle tip of the syringe, and wind force blows droplets squeezed out of the syringe.

[0009] Preferably, the injection pump is a micro peristaltic injection pump.

[0010] Furthermore, the injection pump further comprises a screw shaft, a screw shaft support block, a fastening knob, a syringe push block, a syringe pressure block, and a shaft support block;

[0011] The screw shaft, screw shaft support block, fastening knob, syringe push block, syringe pressure block, and shaft support block are placed on the top of the injection pump, and the screw shaft support block, syringe push block, and shaft support block are aligned in sequence; the screw shaft support block and the shaft support block are connected through the screw shaft; the screw shaft passes through the syringe push block, so that the syringe push block slides along the screw shaft; the barrel of the syringe is placed on the shaft support block, and the push rod of the syringe is fixed to the syringe push block by the fastening knob.

[0012] Furthermore, the injection pump also includes a knob and a key operation area for adjusting parameters, and the parameters are displayed on a parameter display screen; the knob, parameter display screen and key operation area are arranged on the side of the injection pump.

[0013] Furthermore, the needle tip diameter of the syringe is 0.35 to 0.6 mm.

[0014] A method for preparing sodium alginate gel microspheres, using a device for preparing sodium alginate gel microspheres.

[0015] Furthermore, the preparation method comprises the following steps:

[0016] S1. preparing materials for preparing sodium alginate gel microspheres, including sodium alginate solution and calcium chloride solution;

[0017] S2, add sodium alginate solution into the syringe;

[0018] S3. Set the extrusion rate of the injection pump and the wind speed of the electric blower;

[0019] S4. Start the syringe pump and the electric blower to allow the sodium alginate solution to be steadily squeezed out through the needle tip, then separated from the needle tip and enter the calcium chloride solution. After the cross-linking reaction is completed, sodium alginate gel microspheres are obtained.

[0020] Furthermore, the extrusion rate is 1.6 to 6.8 mm / min.

[0021] Furthermore, the wind speed is 4.5-12 m / s.

[0022] Furthermore, the particle size of the sodium alginate gel microspheres is 100 to 2000 μm.

[0023] Preferably, the needle tip diameter of the syringe is 0.45-0.6 mm, and when the wind speed is 4.5-10 m / s, the particle size of the sodium alginate gel microspheres is 750-1800 μm.

[0024] Preferably, the needle tip diameter of the syringe is 0.35-0.5 mm, and when the wind speed is 9-12 m / s, the particle size of the sodium alginate gel microspheres is less than 1000 μm.

[0025] Furthermore, the materials for preparing sodium alginate gel microspheres also include nanomaterials and drugs.

[0026] Preferably, the drug comprises polypeptides and chemical drugs.

[0027] The present invention combines microfluidic micro-solution extrusion technology with wind-blowing equipment, and constantly squeezes out sodium alginate solution from the needle tip by microfluidic micro-solution extrusion technology. While adjusting the size of the wind-blowing intensity, sodium alginate droplets of different sizes can be blown into a calcium chloride solution from the needle tip, thereby cross-linking to form sodium alginate gel balls in the calcium chloride solution. The present invention is not only simple and efficient, but also can regulate the particle size of the sodium alginate gel balls. The present invention, through microfluidic extrusion technology, achieves accurate control of parameters such as extrusion rate and mixing ratio in the preparation process of sodium alginate gel balls, ensuring the consistency and quality stability of the sodium alginate gel balls.

[0028] The wind-force blowing control technology of the present invention utilizes airflow to control the fluid flow in the microfluidic system. Through the wind-force control technology, the blowing speed of the droplets from the needle tip of the microfluidic device is accurately controlled, thereby accelerating the sodium alginate droplets to detach from the needle tip and enter the calcium chloride solution to cross-link into balls. In addition, the present invention realizes automated control by combining the microfluidic preparation system with the wind-force blowing device, which can reduce the error of manual operation, improve production efficiency, and also ensure the uniformity of sample preparation. Using wind-force to control the microfluidic system, there is no need for complex equipment and operating steps, and the preparation cost and labor cost of the sodium alginate gel balls are also reduced. The effect of the microfluidic extrusion device is to ensure the uniformity of the gel ball particle size by uniformly controlling the extrusion flow rate of the sodium alginate solution. The role of the wind-force blowing device is to regulate the blowing speed of the droplets extruded from the microfluidic device, and then regulate the particle size of the prepared sodium alginate gel balls.

[0029] Compared with the prior art, the beneficial effects of the technical solution of the present invention are:

[0030] 1. Precise particle size control and uniform morphology. The present invention successfully achieves precise control of the particle size of sodium alginate gel spheres by adjusting the needle tip diameter. The smaller the needle tip diameter, the smaller the particle size of the sprayed gel spheres, but the sodium alginate solution is more likely to clog the needle tip, and vice versa. By using needle tips of different diameters, gel spheres with average particle sizes ranging from 582 to 1116 μm were prepared, with small particle size errors and uniform shapes. The present invention overcomes the shortcomings of traditional ion crosslinking and spray methods that are difficult to uniformly control particle size and shape.

[0031] 2. Wind speed control enables dynamic adjustment of particle size. By adjusting the wind speed, the particle size can be dynamically controlled between 744 and 1766 μm. The higher the wind speed, the faster the droplets detach from the needle tip, resulting in a smaller particle size; the lower the wind speed, the larger the particle size. This advantage overcomes the difficulty of adjusting particle size with existing electrostatic spraying methods due to the high cost and complexity of the equipment, while also simplifying operation.

[0032] 3. The extrusion rate has little effect on the particle size, and the preparation stability is high. Within the extrusion rate range of the present invention, the particle size of the prepared gel spheres does not change much. Under the conditions of a wind speed of 10.3 m / s and an extrusion speed of 5.545 mm / min, the average diameter remains between 842 and 925 μm. This shows that the microfluidic extrusion device of the present invention can provide stable fluid delivery, avoid particle size fluctuations caused by uneven injection, and thus improve the repeatability and reliability of the preparation.

[0033] 4. High versatility and wide applicability. By adding silica and antimicrobial peptide drugs to a sodium alginate solution, gel microspheres encapsulating nanomaterials and drugs were successfully prepared. Fluorescence observation confirmed that the drugs and materials were effectively encapsulated by the gel microspheres, maintaining their activity and exhibiting a good spherical morphology, demonstrating that this technology is suitable for preparing a variety of gel microsphere types. The device and preparation method of the present invention can meet the needs of various fields, from biomedicine to food processing. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a device for preparing sodium alginate gel microspheres;

[0035] Figure 2 Gel balls ejected from different needle tips;

[0036] Figure 3 Gel balls ejected at different wind speeds

[0037] Figure 4 Gel balls prepared at different extrusion speeds;

[0038] Figure 5Preparation of different types of sodium alginate gel microspheres, a is a stereo microscope photo of alginate gel microspheres encapsulated with silica nanomaterials, b is a FITC-labeled antimicrobial peptide sodium alginate gel microspheres, c is a real-life photo of silica gel spheres, and d is a fluorescence image of FITC gel spheres.

[0039] Figure 1 In the figure, 1: injection pump; 10: screw shaft; 11: screw shaft support block; 12: tightening knob; 13: syringe push block; 14: syringe pressure block; 15: shaft support block; 16: knob; 17: parameter display screen; 18: key operation area; 2: electric blower; 3: syringe. DETAILED DESCRIPTION

[0040] The present invention will be further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0041] Unless otherwise specified, the reagents and materials used in the following examples were commercially available.

[0042] Example 1

[0043] 1. Preparation device of sodium alginate gel microspheres

[0044] like Figure 1 As shown, the preparation device of sodium alginate gel microspheres includes: a syringe pump 1; an electric blower 2; and a syringe 3. The syringe pump also includes: a screw shaft 10; a screw shaft support block 11; a tightening knob 12; a syringe push block 13; a syringe pressure block 14; a shaft support block 15; a knob 16; a parameter display screen 17; and a key operation area 18.

[0045] 2. Preparation of sodium alginate gel microspheres

[0046] Disperse 0.15g of sodium alginate in 10mL of deionized water and stir continuously at 800 rpm for 10 minutes using a magnetic stirrer. Prepare 5L of a 1.5% CaCl2 solution. To facilitate observation of the morphology of the prepared gel spheres, add 1mL of a black Fe3O4 solution dropwise to the 5mL sodium alginate solution and stir at 500 rpm for 10 minutes to obtain a homogeneous mixed solution. Pour the sodium alginate solution into a syringe with a 0.5mm diameter needle tip.

[0047] Use the syringe pressing block 14 to fix the barrel of the syringe 3, adjust the position of the syringe push block 13, and lock the push rod of the syringe 3 with the tightening knob 12. Set the extrusion rate of the syringe pump 1 and the wind speed parameters of the electric blower 2 through the key operation area 17. Set the wind speed to 10.3m / s and the extrusion speed to 5.545mm / min. Start the syringe pump 1, and the sodium alginate solution is stably extruded through the needle tip of the syringe 3. At the same time, start the electric blower 2, and the wind blows the extruded droplets, causing them to detach from the needle tip and enter the calcium chloride solution. After the droplets enter the calcium chloride solution, the calcium ions react with the sodium alginate to form sodium alginate gel balls.

[0048] Example 2

[0049] The technical solution of Example 2 is similar to that of Example 1, except that needle tips with diameters of 0.35 mm, 0.45 mm, and 0.6 mm are used respectively.

[0050] Example 3

[0051] The technical solution of Example 3 is similar to that of Example 1, except that, when the extrusion speed is set to 5.045 mm / min, the wind speeds are 4.9 m / s, 6.2 m / s, 7.1 m / s, 9.2 m / s, 10.0 m / s, and 12.0 m / s, respectively.

[0052] Example 4

[0053] The technical solution of Example 4 is similar to that of Example 1, except that, when the wind speed is constant at 5.045 m / s, the extrusion speeds are 1.680 mm / min, 2.520 mm / min, 3.360 mm / min, 4.200 mm / min, 5.045 mm / min, and 6.725 mm / min, respectively.

[0054] Example 5

[0055] The technical solution of Example 5 is similar to that of Example 1, except that silicon dioxide and fluorescein isothiocyanate (FITC)-labeled antimicrobial peptide drugs are added to the sodium alginate solution.

[0056] Example 6

[0057] 1. Preparation of sodium alginate gel microspheres

[0058] Disperse 0.15 g of sodium alginate in 10 mL of deionized water and stir continuously at 800 rpm using a magnetic stirrer for 10 minutes. Prepare 5 L of a 1.5% CaCl2 solution.

[0059] Syringe pump 1 is activated, and the sodium alginate solution is steadily extruded through the needle tip (0.5 mm) of syringe 3 (at an extrusion speed of 5.545 mm / min). Simultaneously, blower 2 is activated (at a wind speed of 10.3 m / s), blowing the extruded droplets away from the needle tip and into the calcium chloride solution. Once the droplets enter the calcium chloride solution, the calcium ions cross-link with the sodium alginate, forming sodium alginate gel spheres.

[0060] Result Analysis

[0061] 1. The influence of needle tip size on the particle size of gel sphere preparation

[0062] When the extrusion rate remains constant, the effect of needle tip diameter on the extrusion flow rate can be analyzed using the fundamental principle of fluid mechanics: extrusion flow rate Q = A / cdot v. Here, Q represents the extrusion flow rate, A represents the cross-sectional area of ​​the needle tip, and v represents the flow velocity of the fluid through the extrusion nozzle. Since the flow velocity v is fixed when the extrusion rate remains constant, the extrusion flow rate Q primarily depends on the cross-sectional area A of the needle tip. The cross-sectional area A of the needle tip changes with the tip diameter D. This indicates that the extrusion flow rate Q is proportional to the square of the tip diameter D of the extrusion nozzle. As the tip diameter D decreases, the extrusion flow rate Q also decreases, and vice versa.

[0063] like Figure 2 As shown, the gel spheres prepared in Examples 1 and 2 were photographed and measured under a stereo microscope, and the particle size values ​​of the microspheres were statistically analyzed. The average diameters of the gel spheres ejected using syringes with needle tip diameters of 0.35 mm, 0.45 mm, 0.5 mm, and 0.6 mm were 582.062 ± 5.37 μm, 785.79 ± 8.80 μm, 880.700 ± 10.61 μm, and 1116.430 ± 7.11 μm, respectively. Therefore, from the above statistical data, it can be seen that the particle size of the prepared sodium alginate gel spheres can be regulated by adjusting the needle tip of different thicknesses. The thicker the needle tip, the larger the particle size of the sodium alginate gel spheres ejected, and vice versa.

[0064] 2. Effect of blowing wind speed on the preparation of sodium alginate gel balls

[0065] The increase in wind speed can accelerate the detachment of sodium alginate droplets from the needle tip, thereby regulating the size of the sodium alginate gel ball to form a particle size and the shape of the sphere. In Example 3, when the wind speeds were 4.9m / s, 6.2m / s, 7.1m / s, 9.2m / s, 10.0m / s, and 12.0m / s, the average diameters of the gel microspheres ejected were 1766.720±23.17μm, 1606.410±55.81μm, 1146.041±13.27μm, 978.94±19.87μm, 835.42±14.73μm, and 744.15±1.83μm, respectively. Therefore, the regulation of the size of the sodium alginate gel ball particle size can be achieved by adjusting the size of the wind speed blowing. In the range of wind speed of 4.9 to 12.0 m / s, the greater the wind speed, the smaller the particle size of the prepared sodium alginate gel spheres, and vice versa.

[0066] 3. Effect of extrusion rate on particle size of sodium alginate gel spheres

[0067] The extrusion speed of the microinjection pump directly affects the injection pressure and the dynamic characteristics of the fluid during injection. A higher extrusion speed usually leads to a higher injection pressure, thereby increasing the flow rate and impact force of the liquid. Under the combined action of wind blowing, gel balls with smaller particle sizes can be formed. The average diameters of the sodium alginate gel microspheres containing Fe3O4 prepared in Example 4 are 842.65±4.46μm, 749.29±8.48μm, 782.80±11.27μm, 925.79±18.8μm, 880.95±10.11μm, and 880.94±10.03μm, respectively. From the statistical results of the experimental data, the extrusion speed of the microinjection pump has little effect on the particle size and shape of the sodium alginate gel microspheres in the range of 1.680~6.725mm / min.

[0068] 4. Preparation of different types of sodium alginate gel microspheres

[0069] The structure of a hydrogel is similar to a three-dimensional network, formed by cross-linking polymer chains. This network structure can form a large number of pores and voids, which can accommodate and encapsulate various substances. Hydrogels generally have a strong hydrophilicity, which enables them to interact well with water-soluble substances. The hydrophilicity enables the gel to effectively dissolve and encapsulate a variety of nanomaterials or drugs. In Example 5, the efficacy of preparing various types of sodium alginate gel microspheres based on wind-powered microfluidics of the present invention was tested.

[0070] like Figure 5 As shown, the sodium alginate solution mixed with silica and FITC-labeled antimicrobial peptide drugs can be successfully used to prepare sodium alginate composite gel microspheres with good morphology ( Figure 5a~c). Figure 5 As shown in Figure d, the bright green FITC fluorescence signal observed in the gel microspheres indicates that the antimicrobial peptide drug is successfully encapsulated by the sodium alginate microspheres. This demonstrates that the preparation technology of sodium alginate gel microspheres proposed in this invention has wide applicability and can meet the preparation requirements of various types of sodium alginate gel microspheres.

[0071] 5. Cross-linking principle and particle size control of microfluidic sodium alginate gel sphere technology based on wind power regulation

[0072] Taking Example 6 as an example, the presence of calcium ions in the solution promotes electrostatic attraction and hydrophobic force between sodium alginate molecules, so that the sodium alginate molecules form a network structure; secondly, the calcium ions interact electrostatically with the negative charge of the carboxyl group in sodium alginate, thereby forming a three-dimensional network structure of sodium alginate gel. In Example 6, the sodium alginate gel ball technology is controlled by microflow control regulated by wind power, and the solution is extruded by microflow control, and the sodium alginate droplets are blown into the calcium chloride solution using the method of wind power blowing and then cross-linked into balls. The sodium alginate gel balls prepared by this technology have a particle size controllable characteristic within a certain range and have a high degree of monodispersity. It is an ideal method for constructing sodium alginate gel microspheres in the size range of 100 microns to 1000 microns, thereby providing technical support for the application of sodium alginate gel microspheres in the fields of food processing, biomedicine, etc.

[0073] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Those skilled in the art will appreciate that other variations or modifications can be made based on the above description. It is not necessary and impossible to enumerate all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the claims of the present invention.

Claims

1. A preparation device for sodium alginate gel microspheres, characterized in that: include: An injection pump (1), an electric blower (2), and a syringe (3); the syringe (3) and the electric blower (2) are placed on the top of the injection pump (1); a push rod of the syringe (3) is connected to the injection pump (1); the injection pump (1) drives the push rod to move; an air outlet of the electric blower (2) faces the needle tip of the syringe (3), and wind force blows liquid droplets squeezed out of the syringe (3).

2. The preparation device of sodium alginate gel microspheres according to claim 1, characterized in that: The injection pump (1) further comprises a screw shaft (10), a screw shaft support block (11), a tightening knob (12), a syringe push block (13), a syringe pressure block (14), and a shaft support block (15); The screw shaft (10), the screw shaft support block (11), the tightening knob (12), the syringe push block (13), the syringe pressure block (14), and the shaft support block (15) are placed on the top of the injection pump (1), and the screw shaft support block (11), the syringe push block (13), and the shaft support block (15) are sequentially connected and aligned; the screw shaft support block (11) and the shaft support block (15) are connected via the screw shaft (10); the screw shaft (10) passes through the syringe push block (13), so that the syringe push block (13) slides along the screw shaft (10); the barrel of the syringe (3) is placed on the shaft support block (15), and the push rod of the syringe (3) is fixed to the syringe push block (13) via the tightening knob (12); the syringe pressure block (14) is placed on the shaft support block (15) and is used to fix the barrel of the syringe (3).

3. The preparation device of sodium alginate gel microspheres according to claim 1, characterized in that: The injection pump (1) further comprises a knob (16) and a key operation area (18) for adjusting parameters, and the parameters are displayed on a parameter display screen (17); the knob (16), the parameter display screen (17) and the key operation area (18) are arranged on the side of the injection pump (1).

4. The preparation device of sodium alginate gel microspheres according to claim 1, characterized in that: The needle tip diameter of the syringe (3) is 0.35-0.6 mm.

5. A method for preparing sodium alginate gel microspheres, characterized in that: A device for preparing sodium alginate gel microspheres according to any one of claims 1 to 4.

6. The method for preparing sodium alginate gel microspheres according to claim 5, characterized in that: The following steps are involved: S1. preparing materials for preparing sodium alginate gel microspheres, including sodium alginate solution and calcium chloride solution; S2, adding sodium alginate solution into the syringe (3); S3, setting the extrusion rate of the injection pump (1) and the wind speed of the electric blower (2); S4, starting the syringe pump (1) and the electric blower (2), so that the sodium alginate solution is stably squeezed out through the needle tip, then separated from the needle tip and enters the calcium chloride solution, and the droplets undergo a cross-linking reaction to obtain sodium alginate gel microspheres.

7. The method for preparing sodium alginate gel microspheres according to claim 6, characterized in that: The extrusion rate is 1.6-6.8 mm / min.

8. The method for preparing sodium alginate gel microspheres according to claim 6, characterized in that: The wind speed is 4.5-12 m / s.

9. The method for preparing sodium alginate gel microspheres according to claim 6, characterized in that: The particle size of the sodium alginate gel microspheres is 100 to 2000 μm.

10. The method for preparing sodium alginate gel microspheres according to claim 6, characterized in that: Materials for preparing sodium alginate gel microspheres also include nanomaterials and drugs.

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