Pipettor capable of regulating and controlling number of gun heads and use method of pipettor

Abstract

The invention relates to the field of pipettors, aims to solve the problem that the number of pipette heads cannot be adjusted when an existing pipette is used for transferring liquid, and provides a pipette capable of adjusting and controlling the number of the pipette heads, the pipette comprises a containing seat, the pipette heads, a rotating shaft pressing rod, a suction head pressing plate and a second elastic piece, the containing seat is provided with a shaft hole, and the hole wall of the shaft hole is provided with a containing groove in the length direction of the shaft hole; an opening is formed in the bottom wall of the containing groove, a first elastic piece is arranged on the bottom wall of the containing groove, and mounting plates are arranged at the two ends, close to the bottom, of the containing seat; a containing groove is formed in the installation plate, a gun head is installed in the containing groove, the rotating shaft pressing rod penetrates through the through hole and is provided with a retracting groove, the suction head pressing plate is movably connected with the installation plate and has the limiting motion degree in the vertical direction, and the suction head pressing plate is provided with a receding hole. The number of the collected gun heads can be adjusted through the collecting grooves with different lengths in the rotating shaft pressing rod, and then the use number of the gun heads is adjusted; the suction head pressing plate is used for pressing the non-assembled suction head, so that the non-assembled suction head is prevented from being taken up.

Description

Technical Field

[0001] The present invention relates to the field of pipettes, and in particular to a pipette capable of adjusting the number of gun tips and a method of using the pipette. Background Art

[0002] A pipette is a type of pipette, which is often used in laboratories to transfer a small amount or trace amount of liquid. Different sizes of pipettes are used in conjunction with different sizes of gun tips. It is widely used in the fields of immunology, biochemistry, clinical diagnosis and food analysis. In order to move the liquid in multiple test tubes at a time, the existing pipette is often equipped with multiple gun tips, mainly 8 channels and 16 channels. For example, the patent with the publication number CN221132309U discloses a row gun pipette, which has a fixed number of gun tips and a fixed number of test tubes that can be transferred each time, and has low applicability. Since the channels in the existing pipettes are all sucked or spitted at the same time, when using an 8-channel pipette to transfer the liquid in 6 test tubes, it is also necessary to use the other two gun tips at the same time, and the suction tips configured on these two gun tips will be wasted. In addition, since the number of test tubes receiving the liquid is also 6, when the liquid is spitted in the area receiving the liquid, the liquid may be spit outside the deep well plate, or even spit into the holes of other samples, making it difficult to apply. Summary of the invention

[0003] The purpose of the present invention is to provide a pipette with adjustable number of gun tips, so as to solve the problem that the number of gun tips cannot be adjusted when the existing pipette is transferring liquid, and at the same time solve the problem that when the number of suction tips is greater than the number of enabled gun tips due to the adjustable number of gun tips, unassembled suction tips may be carried away by assembled suction tips.

[0004] The embodiments of the present invention are implemented by the following technical solutions:

[0005] A pipette capable of adjusting the number of gun tips, comprising: a receiving seat, a gun tip, a rotating shaft pressure rod, a suction tip pressure plate and a second elastic member, the receiving seat being provided with an axial hole, the hole wall of the axial hole being provided with a plurality of receiving grooves for receiving the gun tip along its length direction, the bottom wall of the receiving groove being provided with an opening for the end of the gun tip to pass through, the bottom wall of the receiving groove being provided with a first elastic member, and both ends of the receiving seat near the bottom being provided with mounting plates; the gun tip is installed in the receiving groove, and one end of the first elastic member away from the bottom wall of the receiving groove is connected to the gun tip; the rotating shaft pressure rod is passed through the through hole and can rotate in the through hole The rotating shaft pressure rod is movable, and a plurality of stowage grooves are arranged in sequence along its circumference. Stowage grooves of different lengths can accommodate different numbers of gun heads. The stowage grooves that can accommodate an even number of gun heads or an odd number of gun heads are all arranged in the middle part of the length direction of the rotating shaft pressure rod; the suction head pressing plate is movably connected to the mounting plate and has a limited degree of movement in the vertical direction, and the suction head pressing plate is provided with a clearance hole for the gun head to pass through; the second elastic member is arranged between the mounting plate and the suction head pressing plate, and when the suction head removal block and the suction head pressing plate undergo relative displacement in the vertical direction, the second elastic member is in a compressed state.

[0006] Preferably, there are a plurality of the second elastic members, and the plurality of the second elastic members are equally distributed on the two mounting plates.

[0007] Preferably, the mounting plate is provided with a connecting hole, guide rods are provided at both ends of the suction head pressing plate, the guide rods are provided with threaded holes, and the free ends of the guide rods pass through the connecting holes and are connected to the screws.

[0008] Preferably, the second elastic member is a spring, and there are two of the second elastic members; the free ends of the two guide rods respectively pass through one of the second elastic members and are then connected to the screws.

[0009] Preferably, adjacent stowage grooves are connected to each other in the width direction thereof, and the shaft pressure rod has at least one non-groove area, and the non-groove area is used to press down all the gun heads.

[0010] A method for using the pipette, comprising:

[0011] S100, rotating the shaft pressure rod according to the number of gun heads that need to be activated, so that a corresponding number of gun heads move downward;

[0012] S200, after aligning the gun tip with the corresponding suction tip, move the pipette downward so that the gun tip and the suction tip are assembled, and then move the pipette to the area to be aspirated.

[0013] Preferably, the number of tips in each row is greater than or equal to the total number of tips on the pipette; the method of use also includes a method for regulating the moving position of the pipette, the regulating method comprising:

[0014] D100, determine the number of unactivated gun heads on both sides of the activated gun head a 1 and a 2 ;

[0015] D200, if a 1 =a 2 , then move the pipette so that all its tips are aligned with the suction tip; if a 1 with a 2 If the difference is 1, move the pipette so that the tips at the middle end of the side with more inactivated tips are outside the tip placement area, and the remaining tips are aligned with the corresponding tips.

[0016] Preferably, two adjacent tips at one end of each row of tips are respectively the first tip and the second tip, and the first tip is the end tip; a tip at one end of the pipette is the first tip, and a tip at the other end is the Nth tip;

[0017] Before the gun tip and the suction head are aligned in D200, the position information of the first suction head and the second suction head on the horizontal plane is collected; if a 1 =a 2 , then move the pipette so that the position information of the first gun tip is consistent with the position information of the first suction tip; if a 1 with a 2 If the difference is 1, the pipette is moved so that the position information of the first pipette tip or the Nth pipette tip is consistent with the position information of the second pipette tip.

[0018] Preferably, before the pipette moves, the first gun tip and / or the Nth gun tip are located at a preset position, and then according to a 1 with a 2 The final position of the first gun tip or the Nth gun tip after moving is determined by comparing the information of the preset position with the final position, and the distance information of the longitudinal movement and / or lateral movement on the horizontal plane of the pipette is obtained, and the pipette is moved according to the distance information.

[0019] Preferably, after each suction head assembly is completed, the position information of the remaining suction heads in the corresponding row is collected and the number of suction heads arranged continuously n is determined. 1 and n 2 ; Before assembling the suction head next time, compare n 1 and n 2 The number of gun heads to be activated is m, when n 1 and n 2 When all are less than m, the gun head is assembled with the new row of suction heads; when n 1 and n 2 When at least one of the numbers is greater than or equal to m, the gun tip is assembled with the remaining tips.

[0020] The present invention has at least the following beneficial effects:

[0021] The pipette provided by the present invention can adjust the number of folded gun tips through the folding grooves of different lengths on the rotating shaft pressure rod, and thus adjust the number of gun tips in use. For example, when the folding groove that can accommodate two gun tips is rotated to above the gun tips, under the action of the elastic member, the two gun tips retract into the folding groove, and after the remaining six gun tips are assembled with the straws, six test tubes can be pipetted. Since the number of gun tips is adjustable, a row of tips may be left over when the gun tips on the pipette are assembled with the tips on the deep well plate. During the process of inserting the gun tips into the tips during assembly, the assembled tips will swell to a certain extent due to the tight fit between the two, and thus the remaining tips may be carried up by the assembled tips. Therefore, the present invention also provides a tip pressing plate and a second elastic member. When the assembled tips are moved up, the tip pressing plate can be pressed down by the resilience of the second elastic member, and the tip pressing plate can press down the unassembled tips, thereby preventing the unassembled tips from being carried up. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0023] Figure 1 It is a schematic diagram of the structure of a pipette with adjustable number of pipette tips;

[0024] Figure 2 for Figure 1 A schematic diagram of the structure of the middle accommodating seat;

[0025] Figure 3 for Figure 2 A cross-sectional view of

[0026] Figure 4 A first layout diagram for stowing the slot;

[0027] Figure 5 A second layout diagram for stowing the slot;

[0028] Figure 6 The third layout diagram for stowing the slot;

[0029] Figure 7 The fourth layout diagram for stowing the slot;

[0030] Figure 8 The fifth layout diagram for stowing the slot;

[0031] Fig. 9 The sixth layout diagram for stowing the slot;

[0032] Fig.10The seventh layout diagram for stowing the slot;

[0033] Fig.11 This is a schematic diagram of the installation of the suction head pressing plate;

[0034] Fig.12 for Fig.11 A cross-sectional view of

[0035] Fig.13 This is a schematic diagram of the first positioning of the gun tip;

[0036] Fig.14 This is a schematic diagram of the second positioning of the gun tip;

[0037] Fig.15 This is a schematic diagram of the third positioning of the gun tip;

[0038] Icon: 1- accommodating seat, 11- shaft hole, 111- accommodating groove, 1111- opening, 1112- first elastic member, 12- mounting plate, 121- connecting hole, 1211- large hole section, 1212- small hole section, 2- gun head, 21- insertion section, 22- limiting section, 3- rotating shaft pressure rod, 31- folding groove, 32- non-groove area, 33- bearing, 34- working section, 35- connecting section, 4- suction head pressure plate, 41- guide rod, 5- gear, 6- screw, 7- second elastic member, 8- deep hole plate, 9- suction head, 10- motor. DETAILED DESCRIPTION

[0039] In order to make the purpose, method scheme and advantages of the embodiments of the present invention clearer, the method scheme in the embodiments of the present invention is clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0040] Example 1: Figure 1-3 and Figure 11-12As shown, a pipette with adjustable number of gun tips comprises: a receiving seat 1, a gun tip 2, a rotating shaft pressure rod 3, a tip pressure plate 4 and a second elastic member 7, the receiving seat 1 is provided with an axial hole 11, the hole wall of the axial hole 11 is provided with a plurality of receiving grooves 111 for receiving the gun tip 2 along its length direction, the bottom wall of the receiving groove 111 is provided with an opening 1111 for the end of the gun tip 2 to pass through, the bottom wall of the receiving groove 111 is provided with a first elastic member 1112, and mounting plates 12 are provided at both ends of the receiving seat 1 near the bottom; the gun tip 2 is installed in the receiving groove 111, and one end of the first elastic member 1112 away from the bottom wall of the receiving groove 111 is connected to the gun tip 2; the rotating shaft pressure rod 3 is penetrated by the receiving groove 111, and the first elastic member 1112 is connected to the gun tip 2. The through hole can be rotated in the through hole, the rotating shaft pressure rod 3 is provided with a plurality of stowage grooves 31 arranged in sequence along its circumference, and different lengths of stowage grooves 31 can accommodate different numbers of gun heads 2, and the stowage grooves 31 that can accommodate an even number of gun heads 2 or an odd number of gun heads 2 are all arranged in the middle part of the length direction of the rotating shaft pressure rod 3; the suction head pressing plate 4 is movably connected with the mounting plate 12 and has a limited mobility in the vertical direction, and the suction head pressing plate 4 is provided with a clearance hole for the gun head 2 to pass through; the second elastic member 7 is arranged between the mounting plate 12 and the suction head pressing plate 4, and when the suction head removal head 9 and the suction head pressing plate 4 are relatively displaced in the vertical direction, the second elastic member 7 is in a compressed state.

[0041] In the specific implementation process, the first elastic member 1112 can be a spring, rubber or silicone. The connection between the first elastic member 1112 and the gun head 2 can be a fixed connection or abutment. The first elastic member 1112 can transmit the rebound force to the gun head 2 and make the gun head 2 move. The drive of the rotating shaft pressure rod 3 can be as follows Figure 2-3 As shown, a motor 10 is arranged on the top of the accommodating seat 1, and the output shaft of the motor 10 is connected to the driving gear 5, and then a driven gear 5 is arranged at the end of the rotating shaft pressure rod 3, and the driving gear 5 is connected with the driven gear 5 in a transmission manner, so that the rotating motor 10 can drive the rotating shaft pressure rod 3 to rotate. Figure 3 As shown, it can be adapted to the gun head 2. In order to increase the stability of the gun head 2 during movement, a slider can be set on the side wall of the gun head 2, and a slide groove that cooperates with the slider can be set on the groove wall of the accommodating groove 111. For example, a hose connector can be set on the back of each gun head 2 to facilitate the suction and discharge of liquid. The number and length of the retracting groove 31 can be set according to the number of gun heads 2 that need to be regulated and the width of the gun head 2, etc., and this embodiment does not make specific limitations. Figure 1 The moving mechanism of the pipette is shown in the figure. The moving mechanism is not an improvement of this embodiment. It can be set with reference to the prior art and will not be described in detail. Figure 1 A shell is provided on the basis of the structure shown to shield the accommodating seat 1 and the motor 10 , etc., so as to reduce the danger caused by the exposure of the rotating motor 10 .

[0042] Exemplary, such as Figure 3 As shown, the gun head 2 may include: an insertion section 21 and a limiting section 22, the first elastic member 1112 may be a spring, the insertion section 21 is inserted into the spring, and the end of the insertion section 21 may pass through the opening 1111; the limiting section 22 is connected to the end of the insertion section 21 away from the opening 1111, the limiting section 22 is located above the spring, and at least a part of the orthographic projection of the limiting section 22 toward the opening 1111 is located outside the spring enclosure area. The cross section of the limiting section 22 may be circular, and the diameter of the maximum cross section of the limiting section 22 is greater than the diameter of the spring, so that the limiting section 22 cannot enter the enclosure space of the spring. During the process of pressing down the gun head 2, the end of the limiting section 22 presses down the spring, and during the rebound process of the gun head 2, the rebound force of the spring also directly acts on the end of the limiting section 22. The end of the limiting section 22 away from the insertion section 21 is spherical. In the process of the gun head 2 entering from one stowage groove 31 into another stowage groove 31, in order to reduce the obstruction or even the occurrence of jamming during the movement of the gun head 2, the contact surface between the gun head 2 and the wall of the stowage groove 31 during the movement is set to a spherical surface to reduce the obstruction during the sliding of the gun head 2 along the wall of the stowage groove 31.

[0043] Exemplary, such as Figure 3 As shown, in order to increase the installation stability and rotation stability of the rotating shaft pressure rod 3, the rotating shaft pressure rod 3 may include: a working section 34 and a connecting section 35, and the retracting groove 31 is provided in the working section 34; a connecting section 35 is connected to each of the two ends of the working section 34, and the diameter of the connecting section 35 is smaller than that of the working section 34; a bearing 33 is sleeved on the connecting section 35, and the inner ring of the bearing 33 is connected to the working section 34, and the outer ring of the bearing 33 is connected to the accommodating seat 1. The diameter of the connecting section 35 is smaller than that of the working section 34, and the bearing 33 can be limited to the side close to the working section 34 through the end of the working section 34, and a retaining ring can be provided on the side of the bearing 33 away from the working section 34 to further improve the limiting effect. In addition, a groove that can be embedded in the outer ring of the bearing 33 can be provided on the hole wall of the shaft hole 11 to facilitate the installation and fixation of the outer ring of the bearing 33.

[0044] Exemplary, such as Figure 3 As shown, taking eight gun heads 2 as an example, when only two gun heads 2 are needed, the rotating shaft pressing rod 3 is rotated to rotate the stowage groove 31 that can accommodate six gun heads 2 to the top of the gun heads 2. Under the rebound effect of the first elastic member 1112, the gun heads 2 enter the stowage groove 31. Figure 3 As shown, only two gun heads 2 are pressed down by the shaft pressing rod 3, and then Figure 1As shown, the pipette is moved to the corresponding position on the deep-well plate 8, on which the suction tip 9 is placed. After the pipette is pressed down, the gun tip 2 and the suction tip 9 can be assembled. Since only two gun tips 2 protrude downward, only two gun tips 2 and the suction tip 9 are assembled during the downward movement of the pipette, which can avoid the waste of disposable suction tips 9. Finally, liquid transfer can be achieved according to conventional operations.

[0045] Under normal circumstances, the number of suction tips 9 placed on the deep well plate 8 needs to match the number of gun tips 2 on the pipette. For example, there are 8 gun tips 2 on the pipette, and the number of suction tips 9 in each row on the deep well plate 8 is also 8, so that the suction tips 9 can be taken in order by row. However, in the present embodiment, the number of gun tips 2 of the pipette is controllable, and it is difficult to ensure the sequential use of suction tips 9. For example, when only 6 suction tips 9 are taken, there will be 2 gun tips 2 left in the corresponding row. There will be a problem at this time: if the spacing between each row of suction tips 9 is too small, during the assembly process of the gun tip 2 and the suction tip 9, the tight fit between the two will cause the suction tips 9 to swell to a certain extent, thereby causing the assembled suction tips 9 to abut and squeeze against the unassembled suction tips 9. In the process of the assembled suction tips 9 moving up, the unassembled suction tips 9 in the same row may be brought up. In order to solve the above problem, it is necessary to increase the spacing between adjacent suction tips 9 in each row, thereby increasing the occupied area of ​​the deep well plate 8 and the distance between adjacent gun tips 2 on the pipette. In order to avoid increasing the occupied area of ​​the deep well plate 8 and the spacing between the gun tips 2, the applicant further conceived a technical solution of providing a tip pressing plate 4 on the pipette.

[0046] In the specific implementation process, the limited mobility means that the suction head pressing plate 4 can move in the vertical direction, but the movement range is limited. There are many ways to connect the second elastic member 7, such as Fig.12 As shown, the top end of the second elastic member 7 is connected to the mounting plate 12, and the bottom end is used to abut against the suction head pressing plate 4. Of course, the bottom end of the second elastic member 7 can also be press-connected to the suction head 9, and the top end is used to abut against the mounting plate 12. The top end of the second elastic member 7 can also be connected to the mounting plate 12, and the bottom end of the second elastic member 7 is connected to the suction head pressing plate 4. The second elastic member 7 can be made of spring, rubber or silicone. The elastic coefficient of the second elastic member 7 should not be too large to avoid excessive resilience of the second elastic member 7, which causes the suction head 9 to deform under pressure.

[0047] When using, see Figure 1As shown, after placing the suction tip 9 on the deep well plate 8, the pipette is moved downward to drive the suction tip pressing plate 4 to move downward. When the suction tip pressing plate 4 abuts against the end of the suction tip 9, the pipette continues to move downward to drive the gun head 2 to move downward. At this time, the second elastic member 7 is gradually compressed until the gun head 2 moves down to be assembled with the suction tip 9. Since the gun head 2 and the suction tip 9 are in a tight fit relationship, the suction tip 9 will swell to a certain extent during the process of inserting the gun head 2 into the suction tip 9. The assembled suction tip 9 and the unassembled suction tips 9 around it are squeezed and abutted against each other. At this time, the pipette is moved upward so that the assembled suction tip 9 moves together with the pipette. Under the action of the rebound force of the second elastic member 7, the suction tip pressing plate 4 will press down the unassembled suction tip 9 abutting against the suction tip pressing plate 4 to prevent the unassembled suction tip 9 from being lifted by the assembled suction tip 9.

[0048] For example, Figure 5 As shown, a spacing is provided between the stowage grooves 31, or Figure 4 As shown, adjacent stowage grooves 31 are connected to each other in the width direction. In addition, each stowage groove 31 can be as shown in FIG. Figure 4 As shown, it is a connected slot body, through which multiple gun heads 2 can be accommodated at the same time, and it can also be as shown in FIG. Figure 5 As shown, a stowage slot 31 can be composed of a plurality of sub-slots arranged laterally, each sub-slot accommodating a gun head 2, which can be regarded as a slot body provided with a plurality of partitions to separate the gun heads 2 into a plurality of sub-slots. Taking 8 gun heads 2 as an example, when the stowage slot 31 directly above the gun heads 2 has two sub-slots, only two gun heads 2 are stowed, thereby enabling 6 gun heads 2. Whether to use a slot body or a sub-slot can be determined according to the interval between adjacent gun heads 2. For example, when the interval between adjacent gun heads 2 is large, in order to ensure the movement stability of each gun head 2 and reduce the space in which it can move laterally, the following method can be used: Figure 5 The stowage groove 31 shown is formed of a plurality of sub-grooves. When the interval between adjacent gun heads 2 is small, the following can be adopted: Figure 4 As shown, each stowage slot 31 adopts a slot body.

[0049] If you use Figure 6 In the arrangement of the retracting groove 31 shown in the figure, during the rebound of the suction head pressing plate 4 under the action of the second elastic member 7, the number of unassembled suction heads 9 acting on both sides thereof is different, which may cause a certain degree of deflection of the suction head pressing plate 4. Therefore, in order to better balance the force on the suction head pressing plate 4, the setting position of the retracting groove 31 can be referred to Figure 4-5 As shown in the configuration, when the number of gun tips 2 is an even number, the number of gun tips 2 that can be accommodated in each storage slot 31 is 2, 4, 6, 8, ... in sequence. When the number of gun tips 2 is an odd number, the number of gun tips 2 that can be accommodated in each storage slot 31 is 1, 3, 5, 7, ... in sequence. Of course, in order to ensure that the forces on both ends of the suction head pressing plate 4 are as uniform as possible and to achieve the continuity of the control of the number of gun tips 2, it is also possible to Figure 7-9As shown, each stowage groove 31 is centered in the length direction of the rotating shaft pressure rod 3 as much as possible. For example, when the total number of gun heads 2 is an odd number, such as Figure 7 As shown, the stowage groove 31 that can accommodate an odd number of gun tips 2 is arranged in the center, and the stowage groove 31 that can accommodate an even number of gun tips 2 is arranged at a distance of one sub-groove from the center. When the total number of gun tips 2 is even, the stowage groove 31 that can accommodate an even number of gun tips 2 is arranged in the center, and the stowage groove 31 that can accommodate an odd number of gun tips 2 is arranged at a distance of one sub-groove from the center.

[0050] Exemplarily, the stowage slot 31 may also be as follows Fig.10 As shown, in the process of circumferential arrangement of the pressure rod 3 along the rotating shaft, the length first increases and then decreases, so that when the motor 10 rotates unidirectionally, the continuity of the switching of the number of activations of the gun head 2 can be better achieved.

[0051] Embodiment 2: In order to improve the force uniformity of the suction head pressure plate 4, an improvement is made on the basis of Embodiment 1. In this embodiment, there are multiple second elastic members 7, and the multiple second elastic members 7 are equally distributed on the two mounting plates 12.

[0052] In the specific implementation process, since the number of the pipette tips 2 of the present embodiment is controllable, it is difficult to ensure that the number of the pipette tips 2 at both ends of the enabled pipette tips 2 is the same, and thus it is difficult to ensure that the forces at both ends of the pipette tip pressing plate 4 are balanced. In order to balance the forces at both ends of the pipette tip pressing plate 4 as much as possible, the technical solution of the present embodiment is conceived, wherein the equal amount refers to the same number of the second elastic members 7 at both ends of the mounting plate 12. Fig.12 As shown, two second elastic members 7 may be provided, one second elastic member 7 is provided at each end of the mounting plate 12, so that the forces at the two ends of the suction head pressing plate 4 are as balanced as possible.

[0053] Embodiment 3: In order to increase the stability of the movement of the suction head pressing plate 4, improvements are made on the basis of Embodiment 1, such as Fig.12 As shown, in this embodiment, the mounting plate 12 is provided with a connecting hole 121, and guide rods 41 are provided at both ends of the suction head pressing plate 4. The guide rods 41 are provided with threaded holes, and the free ends of the guide rods 41 pass through the connecting hole 121 and are connected to the screws 6.

[0054] In the specific implementation process, the connection hole 121 may include: a large hole section 1211 and a small hole section 1212, the large hole section 1211 is arranged away from the suction head pressing plate 4; the small hole section 1212 is connected to the large hole section 1211, and the small hole section 1212 is arranged close to the suction head pressing plate 4; the rod of the screw 6 passes through the small hole section 1212 and is threadedly connected with the guide rod 41, the maximum outer diameter of the head of the screw 6 is greater than the aperture of the small hole section 1212, and the maximum outer diameter of the head of the screw 6 is less than the aperture of the large hole section 1211. The cooperation between the guide rod 41 and the screw 6 can not only simply realize the installation plate 12 and the suction head pressing plate 4, but also limit the maximum distance of the relative displacement between the installation plate 12 and the suction head pressing plate 4 through the cooperation between the head of the screw 6 and the connection hole 121. After the screw 6 is installed, the distance between the installation plate 12 and the suction head pressing plate 4 can meet the second elastic member 7 in a compressed state. The diameter of the small hole section 1212 in the connecting hole 121 can be as close as possible to the shaft of the screw 6 to reduce the shaking of the screw 6 in the horizontal direction.

[0055] Can be as Fig.12 As shown, the top end of the second elastic member 7 is connected to the mounting plate 12 to prevent the second elastic member 7 from being affected by the gravity of the suction head pressing plate 4 when both ends of the second elastic member 7 are connected to the mounting plate 12 and the suction head pressing plate 4 respectively. In addition, only one end of the second elastic member 7 is fixedly connected, which is conducive to the detachment of the suction head pressing plate 4 from the mounting plate 12.

[0056] Exemplary, such as Fig.12 As shown, the top end of the second elastic member 7 can be connected to the end of the large hole section 1211, and the bending of the second elastic member 7 can be reduced by limiting the large hole section 1211.

[0057] Embodiment 4: In order to reduce the bending of the second elastic member 7 due to the uneven force on both ends of the suction head pressing plate 4 or to prevent the gun head 2 from being subjected to lateral force, improvements are made on the basis of embodiment 3, such as Fig.12 As shown, in this embodiment, the second elastic member 7 is a spring, and there are two second elastic members 7 ; the free ends of the two guide rods 41 each pass through one of the second elastic members 7 and are connected to the screw 6 .

[0058] During the specific implementation process, after the tip 9 is assembled, when the pipette is moved upward, the interaction force between the tip pressing plate 4 and the unassembled tip 9 is not necessarily symmetrical with respect to the two ends of the tip pressing plate 4. Therefore, after the tip pressing plate 4 is subjected to force and the spring is compressed, the spring may bend. Therefore, in this embodiment, the guide rod 41 passes through the spring, and the bending of the spring can be limited by the guide rod 41, that is, the guide rod 41 not only has at least three functions: first, a connecting piece between the mounting plate 12 and the tip removal pressing plate 4; second, it can increase the movement stability of the tip pressing plate 4; third, it can reduce the bending of the spring or prevent the gun head 2 from being subjected to lateral force.

[0059] Embodiment 5: In order to realize the convenience of controlling the number of gun tips 2, improvements are made on the basis of embodiments 1-4, such as Figure 4-10 As shown, in this embodiment, adjacent stowage grooves 31 are connected to each other in the width direction, and the shaft pressure rod 3 has at least one non-groove area 32, and the non-groove area 32 is used to press down all the gun heads 2.

[0060] In the specific implementation process, the non-groove area 32 refers to a part of the arc surface area on the circumference of the shaft pressure rod 3 where the retracting groove 31 is not provided. When this area is located directly above the gun head 2, the entire gun head 2 is pressed down by the shaft pressure rod 3, so that the gun head 2 is fully activated. Figure 4 or Figure 8-9 When the two gun heads 2 are connected to each other as shown, when the number of gun heads 2 is switched, no one or some of the gun heads 2 will move back and forth, and the rotation angle of the shaft pressure rod 3 is also smaller. When the number of enabled gun heads 2 is switched, the state of some gun heads 2 remains unchanged, and the state of the corresponding first elastic member 1112 remains unchanged, thereby reducing the number of compression and rebound of the first elastic member 1112 during the process of adjusting the number of enabled gun heads 2, thereby reducing the loss of the first elastic member 1112. For example, if the number of enabled gun heads 2 is adjusted from two to four, Figure 5 As shown, there are gaps between the folding grooves 31. When the shaft pressure rod 3 is rotated, the gun head 2 will be pressed down by the shaft pressure rod 3 when it enters another folding groove 31 from one folding groove 31, and then folded under the action of the rebound force of the first elastic member 1112. During the rotation process, the more folding grooves 31 the gun head 2 passes through, the more times the gun head 2 reciprocates, which leads to increased wear of the first elastic member 1112 and the need for frequent replacement. In addition, each time the number of gun heads 2 is adjusted, the angle that the shaft pressure rod 3 needs to rotate increases, and the operation is not convenient enough. Therefore, this embodiment limits the adjacent folding grooves 31 to be connected to each other to solve the aforementioned problem.

[0061] Embodiment 6: This embodiment provides a method for using the pipette, comprising:

[0062] S100, rotating the shaft pressure rod 3 according to the number of gun heads 2 to be started, so that the corresponding number of gun heads 2 move downward;

[0063] S200, after aligning the gun tip 2 with the corresponding suction tip 9, move the pipette downward to complete the assembly of the gun tip 2 and the suction tip 9, and then move the pipette to the area to be aspirated.

[0064] In the specific implementation process, a slide rail for driving the pipette to slide horizontally and a Figure 1For the vertical slide shown, the alignment of the gun tip 2 and the suction tip 9 on the horizontal plane can be achieved in two ways: one is that the suction tip 9 does not move and only the pipette is moved horizontally; the other is that both the suction tip 9 and the pipette are moved, such as the pipette moves horizontally and the suction tip 9 moves longitudinally. The longitudinal movement of the suction tip 9 can be achieved by setting a longitudinal slide, then sliding a sliding plate on the longitudinal slide, and placing a deep-well plate 8 on the sliding plate. Mobile positioning can adopt an existing control system, such as PLC, to achieve precise movement of the pipette or deep-well plate 8 by controlling the operation of the drive motor 10 or displacement sensors. A photoelectric encoder can also be set on the motor 10 or the slide to detect the position and speed in real time, and send the feedback signal to the control system to achieve closed-loop control.

[0065] Embodiment 7: In order to further improve the force uniformity of the suction head pressing plate 4, improvements are made on the basis of Embodiment 6, such as Figure 13-15 As shown, in this embodiment, the number of pipette tips 9 in each row is greater than or equal to the total number of pipette tips 2 on the pipette; the method of use also includes a method for regulating the moving position of the pipette, and the regulation method includes:

[0066] D100, determine the number of unactivated gun heads 2 on both sides of the activated gun head 2 a 1 and a 2 ;

[0067] D200, if a 1 =a 2 , then move the pipette so that all its tips 2 are aligned with the suction tip 9; if a 1 with a 2 If the difference is 1, the pipette is moved so that the tips 2 at the middle end of the side with more inactivated tips 2 are located outside the placement area of ​​the suction tip 9, and the remaining tips 2 are aligned with the corresponding suction tips 9.

[0068] In the specific implementation process, since the position of the activated gun head 2 in this embodiment is located in the middle of the shaft pressure rod 3, when the parity of the total number of gun heads 2 and the number of activated gun heads 2 is different, the number of unactivated gun heads 2 on both sides of the activated gun head 2 is different, for example, see Fig.15As shown, taking 8 gun heads 2 as an example, when only 3 gun heads 2 are enabled, there are 3 unenabled gun heads 2 on the left side and 2 unenabled gun heads 2 on the right side. If there are suction heads 9 under all the unenabled gun heads 2, the forces on both ends of the suction head pressing plate 4 will be not balanced. At this time, two situations may occur: First, if the gun head 2 has no limiting effect on the suction head pressing plate 4, for example, there is one clearance hole on the suction head pressing plate 4, which can pass through the strip holes of 8 gun heads 2 at the same time, then the second elastic member 7 may be bent and the suction head pressing plate 4 may be deflected; second, the gun head 2 has a certain limiting effect on the suction head pressing plate 4, for example, the clearance holes on the suction head pressing plate 4 are multiple independent openings that can be just passed through by the gun head 2 and the straw. At this time, under the limitation of the gun head 2, although the suction head pressing plate 4 is unevenly stressed, it may not be deflected under the obstruction of the gun head 2, but the gun head 2 will be subjected to lateral force, and the service life of the gun head 2 is reduced.

[0069] In order to solve the above problem, this embodiment defines that when the difference between a1 and a2 is 1, Fig.15 In the alignment method shown, the pipette is moved so that the leftmost gun tip 2 moves outside the deep well plate 8, that is, there is no suction tip 9 below the leftmost gun tip 2. At this time, in the process of moving the suction tip pressing plate 4 downward, there are two suction tips 9 on both sides abutting against the suction tip pressing plate 4, which improves the uniformity of the force on the suction tip pressing plate 4. Fig.14 As shown, when the number of unused gun tips 2 on both sides is the same, each gun tip 2 is aligned with the suction head 9. Fig.13 As shown, when all the gun heads 2 are activated, they can be all aligned with the suction head 9 according to the prior art.

[0070] Embodiment 8: In order to reduce the amount of position information collected, an improvement is made on the basis of Embodiment 7. In this embodiment, two adjacent tips 9 at one end of each row of tips 9 are respectively the first tip and the second tip, and the first tip is the end tip 9; a gun tip 2 at one end of the pipette is the first gun tip, and a gun tip 2 at the other end is the Nth gun tip;

[0071] Before the gun tip 2 and the suction head 9 are aligned in D200, the position information of the first suction head and the second suction head on the horizontal plane is collected; if a 1 =a 2 , then move the pipette so that the position information of the first gun tip is consistent with the position information of the first suction tip; if a 1 with a 2 If the difference is 1, the pipette is moved so that the position information of the first pipette tip or the Nth pipette tip is consistent with the position information of the second pipette tip.

[0072] In the specific implementation process, the moving distance of the pipette in each direction needs to be determined according to the relative position of the gun tip 2 and the suction tip 9. If the relative position of each gun tip 2 and the suction tip 9 is analyzed in the process of analyzing and judging the moving mode, the amount of data processing is large. Therefore, when placing the suction tip 9, it can be ensured that the distance between each row of adjacent suction tips 9 corresponds to the distance between adjacent gun tips 2 on the pipette. When a gun tip 2 is aligned with a suction tip 9, the remaining gun tips 2 and suction tips 9 are automatically aligned.

[0073] For example, see Figure 13-15 As shown, it is assumed that the gun tip 2 on the far left is the first gun tip, the gun tip 2 on the far right is the Nth gun tip, the suction tip 9 on the far right is the first suction tip, and the second suction tip 9 from right to left is the second suction tip. Figure 13-14 As shown, when the number of unactivated tips 2 on both sides is the same, the position information of the first tip is used as a reference to move the pipette so that the Nth tip is aligned with the first tip. Fig.15 As shown, when the number of unactivated tips 2 on both sides differs by 1, the pipette is moved based on the position information of the second tip to align the Nth tip with the second tip.

[0074] Example 9: In order to further simplify the data processing process, an improvement is made on the basis of Example 8. In this example, before the pipette moves, the first pipette tip and / or the Nth pipette tip are located at a preset position, and then according to a 1 with a 2 The final position of the first gun tip or the Nth gun tip after moving is determined by comparing the information of the preset position with the final position, and the distance information of the longitudinal movement and / or lateral movement on the horizontal plane of the pipette is obtained, and the pipette is moved according to the distance information.

[0075] During the specific implementation process, the position of the pipette is fixed in the initial state, that is, the position information of the first gun tip or the Nth gun tip always remains unchanged. At the same time, the initial position of the deep well plate 8 can also remain unchanged, that is, the position information of the first suction tip and the second suction tip always remains unchanged. After one assembly is completed, the pipette and / or the deep well plate 8 moves to the initial position. At this time, when judging the data, there is no need to re-collect the position information of the gun tip 2 or the suction tip 9 every time. It is only necessary to judge the parity of the total number of gun tips 2 and the number of enabled gun tips 2 to determine which suction tip 9 the first gun tip or the Nth gun tip needs to be aligned with. When Fig.14 As shown, when the parity is the same, move the leftmost gun head 2 to align with the leftmost suction head 9 or move the rightmost gun head 2 to align with the rightmost suction head 9. Fig.15 As shown, when the parity is different, the leftmost gun head 2 is moved to align with the second suction head 9 on the left, or the rightmost gun head 2 is moved to align with the second suction head 9 on the right.

[0076] Example 10: In order to use the pipette tips 9 on the deep well plate 8 as much as possible, improvements are made on the basis of Examples 6-9. In this example, after each pipette tip 9 is assembled, the position information of the remaining pipette tips 9 in the corresponding row is collected and the number of pipette tips 9 arranged continuously n is determined. 1 and n 2 ; Before the next assembly of the suction head 9, first compare n 1 and n 2 The number of gun heads 2 that need to be enabled is m, when n 1 and n 2 When both are less than m, the gun head 2 is assembled with the suction head 9 of a new row; when n 1 and n 2 When at least one of the numbers is greater than or equal to m, the gun tip 2 is assembled with the remaining suction tips 9.

[0077] During the specific implementation, since the number of enabled tips 2 of the pipette in this embodiment is controllable, there may be some tips 9 remaining in a row on the deep-well plate 8 after one use, and the number of remaining tips 9 may not completely meet the number of tips 9 to be used next time. Therefore, in order to use the tips 9 in each row as much as possible, when there are some tips 9 remaining in a row, before the tips 9 are used next time or later, it is first determined whether the number of remaining tips 9 meets the number of tips 9 to be used.

[0078] For example, see Fig.15 As shown, after one use, two suction heads 9 will be left on the left and three suction heads 9 will be left on the right. If the number of suction heads 9 to be taken next time is 2, you can choose to take the suction heads 9 on the left or the right. It is best to choose to take the suction heads 9 on the left to increase the continuous arrangement of the remaining suction heads 9. If the number of suction heads 9 to be taken next time is 4, you can choose a new row of suction heads 9 for taking. In the process of taking the suction heads 9, a fixed number of times or time of taking can be set, or it can be determined whether there are continuously arranged suction heads 9 that meet the number of suction heads to be taken next time, so as to determine whether the suction heads 9 on the deep well plate 8 need to be replenished.

[0079] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A pipette with adjustable number of tips, characterized in that: include: A receiving seat (1), the receiving seat (1) being provided with an axial hole (11), the hole wall of the axial hole (11) being provided with a plurality of receiving grooves (111) for receiving the gun head (2) along its length direction, the bottom wall of the receiving groove (111) being provided with an opening (1111) for the end of the gun head (2) to pass through, the bottom wall of the receiving groove (111) being provided with a first elastic member (1112), and mounting plates (12) being provided at both ends of the receiving seat (1) near the bottom; A gun head (2), the gun head (2) being mounted in the receiving groove (111), and one end of the first elastic member (1112) being away from the bottom wall of the receiving groove (111) being connected to the gun head (2); A rotating shaft pressure rod (3), the rotating shaft pressure rod (3) is inserted into the through hole and can rotate in the through hole, the rotating shaft pressure rod (3) is provided with a plurality of stowage grooves (31) arranged in sequence along its circumference, different lengths of stowage grooves (31) can accommodate different numbers of gun heads (2), and the stowage grooves (31) that can accommodate an even number of gun heads (2) or an odd number of gun heads (2) are all arranged in the middle of the rotating shaft pressure rod (3) in the length direction; A suction head pressing plate (4), the suction head pressing plate (4) is movably connected to the mounting plate (12) and has a limited degree of mobility in the vertical direction, and the suction head pressing plate (4) is provided with a clearance hole for the gun head (2) to pass through; A second elastic member (7), wherein the second elastic member (7) is arranged between the mounting plate (12) and the suction head pressing plate (4), and when the suction head removal block (9) and the suction head pressing plate (4) undergo relative displacement in a vertical direction, the second elastic member (7) is in a compressed state.

2. The pipette with adjustable number of tips according to claim 1, characterized in that: There are a plurality of the second elastic members (7), and the plurality of the second elastic members (7) are equally distributed on the two mounting plates (12).

3. The pipette with adjustable number of tips according to claim 1, characterized in that: The mounting plate (12) is provided with a connecting hole (121), and guide rods (41) are provided at both ends of the suction head pressing plate (4), and the guide rods (41) are provided with threaded holes, and the free ends of the guide rods (41) pass through the connecting holes (121) and are connected to the screws (6).

4. The pipette with adjustable number of tips according to claim 3, characterized in that: The second elastic member (7) is a spring, and there are two second elastic members (7); the free ends of the two guide rods (41) respectively pass through one of the second elastic members (7) and are then connected to the screw (6).

5. The pipette with adjustable number of tips according to any one of claims 1 to 4, characterized in that: Adjacent stowage grooves (31) are connected to each other in the width direction thereof, and the rotating shaft pressing rod (3) has at least one non-groove area (32), and the non-groove area (32) is used to press down all the gun heads (2).

6. A method for using the pipette according to any one of claims 1 to 5, characterized in that: include: S100, rotating the rotating shaft pressure rod (3) according to the number of gun heads (2) to be activated, so that a corresponding number of gun heads (2) move downward; S200, after aligning the gun tip (2) with the corresponding suction tip (9), move the pipette downward so that the gun tip (2) and the suction tip (9) are assembled, and then move the pipette to the area to be aspirated.

7. The method of use according to claim 6, characterized in that: The number of pipette tips (9) in each row is greater than or equal to the total number of pipette tips (2) on the pipette; the method of use also includes a method for regulating the moving position of the pipette, the method comprising: D100, determining the number a1 and a2 of inactivated gun heads (2) on both sides of the activated gun head (2); D200. If a1=a2, move the pipette so that all its gun tips (2) are aligned with the suction tip (9); if the difference between a1 and a2 is 1, move the pipette so that the gun tips (2) at the middle end of the side with a larger number of inactivated gun tips (2) are located outside the placement area of ​​the suction tip (9), and the remaining gun tips (2) are aligned with the corresponding suction tips (9).

8. The method of use according to claim 7, characterized in that: Assume that two adjacent suction heads (9) at one end of each row of suction heads (9) are respectively a first suction head and a second suction head, and the first suction head is an end suction head (9); assume that a gun head (2) at one end of the pipette is a first gun head, and a gun head (2) at the other end is an Nth gun head; Before the gun tip (2) and the suction tip (9) in D200 are aligned, the position information of the first suction tip and the second suction tip on the horizontal plane is first collected; if a1=a2, the pipette is moved so that the position information of the first gun tip is consistent with the position information of the first suction tip; if the difference between a1 and a2 is 1, the pipette is moved so that the position information of the first gun tip or the Nth gun tip is consistent with the position information of the second suction tip.

9. The method of use according to claim 8, characterized in that: Before the pipette moves, the first gun tip and / or the Nth gun tip is located at a preset position, and then the final position of the first gun tip or the Nth gun tip after movement is determined based on the size relationship between a1 and a2. By comparing the information of the preset position with the final position, the distance information of the longitudinal movement and / or lateral movement on the horizontal plane of the pipette is obtained, and the pipette is moved according to the distance information.

10. The method of use according to any one of claims 6 to 9, characterized in that: After each suction head (9) is assembled, the position information of the remaining suction heads (9) in the corresponding row is collected and the number n1 and n2 of suction heads (9) arranged continuously is determined; before the suction head (9) is assembled next time, n1 and n2 are first compared with the number m of gun heads (2) that need to be activated; when n1 and n2 are both less than m, the gun head (2) is assembled with the suction heads (9) of the new row; when at least one of n1 and n2 is greater than or equal to m, the gun head (2) is assembled with the remaining suction heads (9).

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