Camellia-seed oil with repairing, soothing and anti-wrinkle functions as well as preparation method and application of camellia-seed oil

Abstract

The invention belongs to the technical field of camellia seed oil preparation, and particularly relates to camellia seed oil with repairing, soothing and wrinkle resisting functions and a preparation method and application thereof.The preparation method comprises the following steps that 1, camellia seed kernel powder and water are mixed, and a raw material solution is obtained; (2) performing enzymolysis on the raw material solution to obtain an enzymolysis product; (3) continuously adding compound bacteria into the enzymolysis product, fermenting and sterilizing to obtain a fermentation product; (4) centrifuging, distilling and collecting distillate to obtain camellia seed crude oil; (5) preparing an alkalized oil phase; (6) preparing a soap-removed oil phase; and (7) filtering and decolorizing to obtain the camellia seed oil. The prepared camellia seed oil is high in oleic acid content and linoleic acid content, free of foreign flavor and excellent in repairing, relieving and wrinkle resisting effects.

Description

Technical Field

[0001] The invention belongs to the technical field of camellia seed oil production, and in particular relates to camellia seed oil with repairing, soothing and anti-wrinkle functions, and a preparation method and application thereof. Background Art

[0002] Camellia oil, also known as tea tree oil (but be careful not to confuse it with the Australian tea tree oil of the same name), is a natural oil extracted from the seeds of the camellia (Camellia sinensis) flower. Camellia oil is an important ingredient in many high-end and natural cosmetics due to its diverse skin care benefits, and is increasingly used in cosmetics and personal care products.

[0003] Although the application of camellia seed oil in cosmetics has many advantages, it also faces some technical challenges in actual use: 1. Since the crude camellia seed oil after pressing contains a variety of impurities and heavy odor, it not only affects the skin care value and safe storage of camellia seed oil, but also the core of the conventional refining method is "high temperature and high pressure", so the content of active ingredients in the processed camellia seed oil will be reduced; 2. Although camellia seed oil is rich in antioxidants, its unsaturated fatty acid content is relatively high, and it is easily affected by light, air and high temperature, causing oxidation reactions, producing odor or deterioration.

[0004] Chinese patent CN201710114073.7 provides an antibacterial composition and a moisturizing and repairing composition containing camellia seed oil. The preparation method of camellia seed oil is as follows: drying until the moisture content is less than 8%, then peeling and crushing; pressing the crushed camellia seeds at 70°C and 3MPa to obtain crude oil; filtering the crude oil and decolorizing it to obtain the camellia seed oil. However, the camellia seed oil prepared by this method has unsatisfactory color and smell, which will affect the appearance and use experience of cosmetics, and the shelf life is relatively short.

[0005] The reason why camellia oil is highly respected in the field of skin care is mainly because it contains a variety of active ingredients that are beneficial to the skin. These ingredients work together to give camellia oil repair, soothing, anti-wrinkle and anti-inflammatory effects. In particular, the content of oleic acid and linoleic acid, due to their unique biological activity and functions, can directly act on skin cells to help repair, moisturize and protect the skin, and play an important role in skin care. Oleic acid is the most important fatty acid in camellia oil. It has a high content, good permeability and skin affinity, can be quickly absorbed by the skin, help repair the skin barrier function, and enhance the skin's moisturizing ability. Linoleic acid helps regulate the skin's oil secretion, improve skin dryness and sensitivity problems, and at the same time enhance the skin's barrier function and prevent water loss. However, the content of these two active ingredients in camellia oil on the market is low, and the effect of camellia oil is not ideal.

[0006] Therefore, there is an urgent need for camellia seed oil with repairing, soothing and anti-wrinkle effects, as well as a preparation method and application thereof. Summary of the invention

[0007] The purpose of the present invention is to provide camellia seed oil with repairing, soothing and anti-wrinkle effects, and a preparation method and application thereof.

[0008] In order to achieve the above object, the present invention provides the following technical solutions:

[0009] The preparation method of camellia seed oil with repairing, soothing and anti-wrinkle effects comprises the following steps:

[0010] (1) screening camellia seeds to remove impurities, shelling to obtain camellia seed kernels, crushing the camellia seed kernels to obtain camellia seed kernel powder, mixing the camellia seed kernel powder with water to obtain a raw material liquid for later use;

[0011] (2) adding a neutral protease to the raw material solution, and performing enzymolysis at 40-50° C. and pH 6-7 for 1-2 hours; then adding ficin, and performing enzymolysis at 40-50° C. and pH 6-8 for 1-2 hours; then adding a composite protease, and performing enzymolysis at 40-50° C. and pH 6-7 for 3-4 hours; then inactivating the enzyme at 100° C. for 10 minutes to obtain an enzymolysis product;

[0012] (3) adding the composite bacteria to the enzymatic hydrolysis product to carry out fermentation and sterilization to obtain a fermentation product;

[0013] (4) cooling the fermentation product, centrifuging it at 2000 rpm for 15 minutes to obtain free oil and an emulsion layer; distilling the emulsion layer and collecting the distillate to obtain camellia seed crude oil;

[0014] (5) preheating the camellia seed crude oil to 40-43° C., adding a NaOH aqueous solution, stirring at 83-86° C. at a rate of 80-90 r / min for 40-50 min, and centrifuging while hot to obtain an alkalized oil phase;

[0015] (6) In a water washing pot, the alkalized oil phase is heated to 80-85°C, 20-23% by weight of the alkalized oil phase is added with hot water at 75-80°C, stirred for 20-40 minutes, and then allowed to stand and cool, and after separating the aqueous phase, a desoaped oil phase is obtained;

[0016] (7) filtering the desoaped oil phase with filter cloth, filtering the filtered camellia seed oil with a 200 nm ceramic membrane separation device at a temperature of 50-55° C. and an operating pressure of 0.2-0.3 MPa; finally, performing activated carbon decolorization treatment and filtering to obtain camellia seed oil.

[0017] Furthermore, in the step (2), the amount of neutral protease added is 100-110 U / mL of raw material liquid, the amount of ficin added is 80-90 U / mL of raw material liquid; and the amount of composite protease added is 120-130 U / mL of raw material liquid.

[0018] The invention can improve the content of oleic acid and linoleic acid in camellia seed oil by crushing camellia seed kernels and then performing enzymolysis. Gradient enzymolysis by specific enzymes can promote the release of oleic acid and linoleic acid. The traditional high-temperature and high-pressure pressing method may cause oxidation or thermal decomposition of part of unsaturated fatty acids (oleic acid and linoleic acid) to form peroxides and other harmful substances. The enzymolysis process is carried out under mild conditions, avoiding the influence of high temperature and high pressure, reducing the loss of unsaturated fatty acids, and thus better retaining the original content of oleic acid and linoleic acid.

[0019] Furthermore, the composite bacteria in step (3) include Bacillus licheniformis, Bacillus subtilis and Bacillus pumilus; the amount of Bacillus licheniformis is 10 8 -10 9 CFU / mL stock solution; the dosage of Bacillus subtilis is 10 10 -10 11 CFU / mL stock solution; the dosage of Bacillus pumilus is 10 9 -10 10 CFU / mL stock solution.

[0020] Furthermore, in step (3), the fermentation conditions are pH 6.0-6.5, aerobic fermentation at 30-35°C for 18-20 hours.

[0021] The invention can remove the impurities in camellia oil through fermentation treatment, and at the same time can improve the content of active components in camellia oil, and can synergize with oleic acid and linoleic acid to improve the water-locking and repairing effect of camellia oil. The camellia oil prepared by enzymolysis and fermentation treatment has good soothing and anti-wrinkle effects.

[0022] Furthermore, the conditions for distilling and collecting the distillate in step (4) are: heating to 102-112° C. to collect the distillate, and the distillation time is 40-50 min.

[0023] Furthermore, the conditions for distilling and collecting the distillate in step (4) are: heating to 102-112° C. and cooling with circulating water to collect the condensate to obtain the distillate, and the distillation time is 40-50 min.

[0024] Further, camellia seed kernel powder and water are mixed in a weight ratio of 1:(8-10) to obtain a raw material liquid.

[0025] Furthermore, the camellia seed kernels are crushed to less than 200 meshes.

[0026] Further, 16-18 wt% NaOH aqueous solution was added at 1.2-1.5 times the theoretical amount of alkali, and the theoretical amount of alkali was 7.13×10 -4 ×crude oil quality×crude oil acid value.

[0027] The second aspect of the present invention provides camellia seed oil with repairing, soothing and anti-wrinkle effects obtained by the preparation method.

[0028] The third aspect of the present invention provides the use of camellia seed oil having repairing, soothing and anti-wrinkle effects in the preparation of cosmetics.

[0029] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0030] 1. The present invention can increase the contents of oleic acid and linoleic acid in camellia seed oil by crushing camellia seed kernels and then performing enzymolysis.

[0031] 2. The present invention can remove the foreign taste in camellia seed oil through fermentation treatment.

[0032] 3. The present invention can improve the content of active components in camellia seed oil through fermentation treatment, and improve the water-locking and repairing effect of camellia seed oil.

[0033] 4. The camellia seed oil prepared by enzymatic hydrolysis and fermentation treatment of the present invention has good soothing and anti-wrinkle effects. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] The raw materials used in the following examples of the present invention are all commercially available products:

[0036] Bacillus licheniformis was purchased from Shanghai Baocang Microbiology Co., Ltd., product number: SHMCC D10742.

[0037] Bacillus subtilis was purchased from Shanghai Baocang Microbiology Co., Ltd., product number: SHMCC D15537.

[0038] Bacillus pumilus was purchased from Shanghai Baocang Microbiology Co., Ltd., product number: SHMCC D10474.

[0039] Bacillus megaterium was purchased from Shanghai Baocang Microbiology Co., Ltd., product number: SHMCC D11374.

[0040] Bacillus amyloliquefaciens was purchased from Shanghai Collection Microorganisms Co., Ltd., product number: SHMCC D10877.

[0041] Bacillus coagulans was purchased from Shanghai Collection Microorganisms Co., Ltd., product number: SHMCC D10638.

[0042] Composite protease was purchased from Shanghai Ruichu Biotechnology Co., Ltd., product number R12022174.

[0043] Neutral protease, ficin, alkaline protease, bromelain, and papain were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0044] Example 1

[0045] This embodiment provides a method for preparing camellia seed oil having repairing, soothing and anti-wrinkle effects, comprising the following steps:

[0046] (1) screening camellia seeds, removing impurities, shelling to obtain camellia seed kernels, crushing the camellia seed kernels to less than 200 meshes to obtain camellia seed kernel powder, mixing the camellia seed kernel powder and water in a weight ratio of 1:9 to obtain a raw material liquid for later use;

[0047] (2) adding neutral protease to the raw material solution, and performing enzymolysis at 45° C. and pH 6.5 for 1.5 hours; then adding ficin, and performing enzymolysis at 45° C. and pH 7 for 1.5 hours; then adding composite protease, and performing enzymolysis at 45° C. and pH 6.5 for 3.5 hours; then inactivating the enzyme at 100° C. for 10 minutes to obtain an enzymolysis product;

[0048] The amount of neutral protease added was 105 U / mL of raw material solution, the amount of ficin added was 85 U / mL of raw material solution; and the amount of composite protease added was 125 U / mL of raw material solution.

[0049] (3) adding composite bacteria to the enzymatic hydrolyzate to carry out fermentation under the conditions of pH 6.2, aerobic fermentation at 32°C for 19 hours, sterilization, and obtaining a fermentation product; the composite bacteria include Bacillus licheniformis, Bacillus subtilis and Bacillus pumilus; the amount of Bacillus licheniformis is 10 8 CFU / mL stock solution; the dosage of Bacillus subtilis is 10 11 CFU / mL stock solution; the dosage of Bacillus pumilus is 10 10 CFU / mL stock solution.

[0050] (4) After the fermentation product is cooled, centrifuge it at 2000 rpm for 15 minutes to obtain free oil and an emulsion layer; distill the emulsion layer and collect the distillate, the specific operation is: add the emulsion layer to a container with a condenser, heat it to 105° C. and cool the condenser with circulating water, collect the condensate to obtain the distillate, the distillation time is 45 minutes, and the distillate is camellia seed crude oil;

[0051] (5) Preheat the camellia seed crude oil to 42°C and add 17 wt% NaOH aqueous solution which is 1.4 times the theoretical amount of alkali, the theoretical amount of alkali = 7.13 × 10 -4 × crude oil mass × crude oil acid value, stir at 85°C at a rate of 85r / min for 45min, centrifuge while hot to obtain an alkalized oil phase;

[0052] (6) In a water washing pot, the alkalized oil phase is heated to 82°C, 21% of the weight of the alkalized oil phase by 77°C hot water is added, stirred for 30 minutes, and then allowed to stand and cool. After separating the water phase, a desoaped oil phase is obtained;

[0053] (7) filtering the desoaped oil phase with filter cloth, and filtering the filtered camellia seed oil with a 200 nm ceramic membrane separation device at a temperature of 52° C. and an operating pressure of 0.3 MPa; finally, performing activated carbon decolorization treatment and filtering to obtain camellia seed oil.

[0054] Example 2

[0055] This embodiment provides a method for preparing camellia seed oil having repairing, soothing and anti-wrinkle effects, comprising the following steps:

[0056] (1) screening camellia seeds, removing impurities, shelling to obtain camellia seed kernels, crushing the camellia seed kernels to less than 200 meshes to obtain camellia seed kernel powder, mixing the camellia seed kernel powder and water in a weight ratio of 1:10 to obtain a raw material liquid for later use;

[0057] (2) adding neutral protease to the raw material solution, and performing enzymolysis at 40° C. and pH 7 for 1 hour; then adding ficin, and performing enzymolysis at 50° C. and pH 6 for 2 hours; then adding composite protease, and performing enzymolysis at 40° C. and pH 7 for 4 hours; then inactivating the enzyme at 100° C. for 10 minutes to obtain an enzymolysis product;

[0058] The amount of neutral protease added was 100 U / mL of raw material solution, the amount of ficin added was 90 U / mL of raw material solution; and the amount of composite protease added was 120 U / mL of raw material solution.

[0059] (3) adding composite bacteria to the enzymatic hydrolyzate to carry out fermentation under the conditions of pH 6.5, aerobic fermentation at 30°C for 20 hours, sterilization, and obtaining a fermentation product; the composite bacteria include Bacillus licheniformis, Bacillus subtilis and Bacillus pumilus; the amount of Bacillus licheniformis is 10 8 CFU / mL stock solution; the dosage of Bacillus subtilis is 10 10 CFU / mL stock solution; the dosage of Bacillus pumilus is 10 9 CFU / mL stock solution.

[0060] (4) After the fermentation product is cooled, centrifuge it at 2000 rpm for 15 minutes to obtain free oil and an emulsion layer; distill the emulsion layer and collect the distillate, the specific operation is: add the emulsion layer to a container with a condenser, heat it to 110° C. and cool the condenser with circulating water, collect the condensate to obtain the distillate, the distillation time is 40 minutes, and the distillate is camellia seed crude oil;

[0061] (5) Preheat the camellia seed crude oil to 43°C and add 18 wt% NaOH aqueous solution which is 1.2 times the theoretical amount of alkali, the theoretical amount of alkali = 7.13 × 10 -4 × crude oil mass × crude oil acid value. After stirring at 86°C at a rate of 90r / min for 40min, centrifuge while hot to obtain an alkalized oil phase;

[0062] (6) In a water washing pot, the alkalized oil phase is heated to 85°C, 20% of the weight of the alkalized oil phase by 80°C hot water is added, stirred for 20 minutes, and then allowed to stand and cool. After separating the water phase, a desoaped oil phase is obtained;

[0063] (7) filtering the desoaped oil phase with filter cloth, and filtering the filtered camellia seed oil with a 200 nm ceramic membrane separation device at a temperature of 55° C. and an operating pressure of 0.2 MPa; finally, performing activated carbon decolorization treatment and filtering to obtain camellia seed oil.

[0064] Comparative Example 1

[0065] The difference between this comparative example and Example 1 is as follows: alkaline protease is added to the raw material liquid, and enzymolysis is carried out at 45°C and pH 8.5 for 1.5 hours; bromelain is then added, and enzymolysis is carried out at 45°C and pH 7 for 1.5 hours; papain is then added, and enzymolysis is carried out at 45°C and pH 6.5 for 3.5 hours; then the enzyme is inactivated at 100°C for 10 minutes to obtain an enzymolysis product;

[0066] The amount of alkaline protease added was 105 U / mL of raw material solution, the amount of bromelain added was 85 U / mL of raw material solution; and the amount of papain added was 125 U / mL of raw material solution.

[0067] Comparative Example 2

[0068] The difference between this comparative example and Example 1 is that the amount of neutral protease added is 85 U / mL of raw material liquid, the amount of ficin added is 125 U / mL of raw material liquid; and the amount of composite protease added is 105 U / mL of raw material liquid.

[0069] Comparative Example 3

[0070] The difference between this comparative example and Example 1 is that the dosage of the Bacillus licheniformis is 10 11 CFU / mL stock solution; the dosage of Bacillus subtilis is 10 9 CFU / mL stock solution; the dosage of Bacillus pumilus is 10 8 CFU / mL stock solution.

[0071] Comparative Example 4

[0072] The difference between this comparative example and Example 1 is that the composite bacteria includes Bacillus megaterium, Bacillus amyloliquefaciens and Bacillus coagulans; the amount of Bacillus megaterium is 10 8 CFU / mL raw material solution; the dosage of Bacillus amyloliquefaciens is 10 11 CFU / mL stock solution; the dosage of Bacillus coagulans is 10 10 CFU / mL stock solution.

[0073] Comparative Example 5

[0074] The difference between this comparative example and Example 1 is that no fermentation is performed.

[0075] The preparation method of camellia seed oil with repairing, soothing and anti-wrinkle effects comprises the following steps:

[0076] (1) screening camellia seeds, removing impurities, shelling to obtain camellia seed kernels, crushing the camellia seed kernels to less than 200 meshes to obtain camellia seed kernel powder, mixing the camellia seed kernel powder and water in a weight ratio of 1:9 to obtain a raw material liquid for later use;

[0077] (2) adding neutral protease to the raw material solution, and performing enzymolysis at 45° C. and pH 6.5 for 1.5 hours; then adding ficin, and performing enzymolysis at 45° C. and pH 7 for 1.5 hours; then adding composite protease, and performing enzymolysis at 45° C. and pH 6.5 for 3.5 hours; then inactivating the enzyme at 100° C. for 10 minutes to obtain an enzymolysis product;

[0078] The amount of neutral protease added was 105 U / mL of raw material solution, the amount of ficin added was 85 U / mL of raw material solution; and the amount of composite protease added was 125 U / mL of raw material solution.

[0079] (3) After the enzymatic hydrolysate is cooled, it is centrifuged at 2000 rpm for 15 minutes to obtain free oil and an emulsion layer; the emulsion layer is distilled and the distillate is collected. The specific operation is as follows: the emulsion layer is added to a container with a condenser, heated to 105° C. and the condenser is cooled by circulating water, and the condensate is collected to obtain a distillate. The distillation time is 45 minutes, and the distillate is

[0080] (4) Preheat the camellia seed crude oil to 42°C and add 17 wt% NaOH aqueous solution which is 1.4 times the theoretical amount of alkali, the theoretical amount of alkali = 7.13 × 10 -4 × crude oil mass × crude oil acid value, stir at 85°C at a rate of 85r / min for 45min, centrifuge while hot to obtain an alkalized oil phase;

[0081] (5) In a water washing pot, the alkalized oil phase is heated to 82°C, 21% of the weight of the alkalized oil phase by 77°C hot water is added, stirred for 30 minutes and then allowed to stand and cool, and after separating the water phase, a desoaped oil phase is obtained;

[0082] (6) filtering the desoaped oil phase with filter cloth, and filtering the filtered camellia seed oil with a 200 nm ceramic membrane separation device at a temperature of 52° C. and an operating pressure of 0.3 MPa; finally, performing activated carbon decolorization treatment and filtering to obtain camellia seed oil.

[0083] Comparative Example 6

[0084] The difference between this comparative example and Example 1 is that the camellia oil is prepared by referring to the method in patent CN201710114073.7, and the preparation method of the camellia oil is:

[0085] 1. Dry the camellia seeds until the moisture content is less than 8%, then peel them;

[0086] 2. Place the crushed camellia seeds into a low-temperature press for pressing at a temperature of 70°C, a rotation speed of 35r / Min, and a chamber pressure of 3MPa to obtain crude oil;

[0087] 3. After filtering the larger solid particles from the crude oil with filter cloth, the filtered tea oil is further filtered with a 200nm ceramic membrane separation device at a temperature of 50°C and an operating pressure of 0.3MPa;

[0088] 4. The oil filtered by the ceramic membrane is subjected to activated carbon decolorization treatment and filtered to obtain the raw material camellia seed oil of the present invention.

[0089] Performance Testing

[0090] 1. The camellia seed oil prepared in Examples 1-2 and Comparative Examples 1-6 was subjected to GC-MS detection to determine the contents of oleic acid and linoleic acid.

[0091] The headspace solid phase microextraction (HS-SPME) method was used to extract and enrich the camellia oil sample. The extraction fiber head model was 50 / 30μm DVB / CAR / PDMS. Before each use, the extraction fiber was aged for 3 minutes at the GC-MS injection port at 240℃. Accurately weigh 3mL of camellia oil and add it to a 20mL headspace bottle, seal it immediately, and extract it at 40℃ for 20min. After the extraction was completed, the solid phase microextraction fiber needle was removed and then inserted into the GC injection port for desorption (3min, 240℃) and subsequent GC-MS analysis.

[0092] Specifically, the GC detection conditions are as follows: injection port temperature 240°C; carrier gas is 99.999% high-purity helium, flow rate is 1 mL / min, non-split injection; chromatographic column model is HP-5 30m×0.32mm×0.25μm quartz capillary column, programmed temperature conditions: column oven initial temperature 40°C, then increase to 80°C at a rate of 1°C / min; then increase to 250°C at a rate of 20°C / min, and maintain for 10 minutes;

[0093] Specifically, the MS detection conditions were as follows: the ion source was an EI source, the electron energy was 70 eV, the interface temperature was 250°C, the ion source temperature was 230°C, the mass scanning range was 30-540 m / z, the solvent delay was 3 min, and the full scanning mode was used.

[0094] The results are shown in Table 1.

[0095] Table 1 Performance test results

[0096]

[0097] It can be seen from Table 1 that the contents of oleic acid and linoleic acid in the camellia oil prepared in Examples 1-2 are higher than those in Comparative Examples 1-6, and the camellia oil prepared in the prior art also has an odor. The camellia oil in Comparative Example 5, which is not fermented, also has an odor.

[0098] 2. Volunteers aged 25-40 were selected, two volunteers in a group, and 1wt% camellia seed oil was added to commercially available glycerin (Pechoin Glycerin No. 1) for trial use. During the test period, the volunteers used the corresponding product once in the morning and evening after basic cleansing, and did not use other skin care products or cosmetics. The skin moisture content was tested with a skin moisture meter Corneometer CM825 before use and after 8 weeks of use, and the average value of each group was calculated. The results are shown in Table 2.

[0099] Table 2 Repair effect test results (unit: %)

[0100] Before use After 8 weeks of use Example 1 24.8 39.2 Example 2 24.3 37.8 Comparative Example 1 25.5 31.6 Comparative Example 2 24.9 33.1 Comparative Example 3 25.4 32.6 Comparative Example 4 24.2 34.1 Comparative Example 5 24.0 29.3 Comparative Example 6 24.7 27.2

[0101] As shown in Table 2, after adding the camellia oil of Example 1-2 to glycerin for 8 weeks, the water content of the face is greatly improved compared with that before use, indicating that it has a good water-locking effect and has the effect of improving the moisture of the skin, providing a good environment for repairing the sebum film. In particular, the comprehensive performance of Example 1 is the most outstanding, which is mainly due to the synergistic effect between the active components of the camellia oil.

[0102] However, the comparative examples were significantly worse than the examples in terms of corresponding performance tests because they did not adopt the necessary technical solutions. In comparative examples 1-2, the type and amount of enzymes were changed, and it can be seen that the water-locking effect decreased. In comparative example 3, the type and amount of bacteria were changed, and it can be seen that the water-locking effect decreased. In comparative example 5, no fermentation was performed, and the water-locking effect decreased significantly. In comparative example 6, a pressing method was used for preparation, and the water-locking effect was the worst. The above experimental results further prove the importance of the technical solution defined in the present invention for its technical effect.

[0103] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing camellia seed oil having repairing, soothing and anti-wrinkle properties, characterized in that: The following steps are involved: (1) screening camellia seeds to remove impurities, shelling to obtain camellia seed kernels, crushing the camellia seed kernels to obtain camellia seed kernel powder, mixing the camellia seed kernel powder with water to obtain a raw material liquid for later use; (2) adding neutral protease to the raw material solution, and performing enzymolysis at 40-50° C. and pH 6-7 for 1-2 hours; Then add ficin and perform enzymolysis for 1-2 hours at 40-50°C and pH 6-8; Then, add compound protease, and perform enzymolysis for 3-4 hours at 40-50°C and pH 6-7; inactivate the enzyme and obtain an enzymolysis product; (3) adding the composite bacteria to the enzymatic hydrolysis product to carry out fermentation and sterilization to obtain a fermentation product; (4) cooling the fermentation product and centrifuging it to obtain free oil and an emulsion layer; distilling the emulsion layer and collecting the distillate to obtain camellia seed crude oil; (5) preheating the camellia seed crude oil to 40-43° C., adding a NaOH aqueous solution, stirring at 83-86° C. for 40-50 min, and centrifuging while hot to obtain an alkalized oil phase; (6) In a water washing pot, heat the alkalized oil phase to 80-85°C, add 75-80°C hot water, stir and let stand to cool, separate the water phase, and obtain the desoaped oil phase; (7) filtering the desoaped oil phase with filter cloth, filtering the filtered camellia seed oil with a ceramic membrane separation device, and finally performing activated carbon decolorization treatment and filtering to obtain camellia seed oil.

2. The method for preparing camellia oil having the functions of repairing, soothing and anti-wrinkle according to claim 1, characterized in that: In the step (2), the amount of neutral protease added is 100-110 U / mL of raw material liquid, the amount of ficin added is 80-90 U / mL of raw material liquid; and the amount of composite protease added is 120-130 U / mL of raw material liquid.

3. The method for preparing camellia oil having the functions of repairing, soothing and anti-wrinkle according to claim 1, characterized in that: The composite bacteria in step (3) include Bacillus licheniformis, Bacillus subtilis and Bacillus pumilus; the amount of Bacillus licheniformis is 10 8 -10 9 CFU / mL stock solution; the dosage of Bacillus subtilis is 10 10 -10 11 CFU / mL stock solution; the dosage of Bacillus pumilus is 10 9 -10 10 CFU / mL stock solution.

4. The method for preparing camellia oil having the functions of repairing, soothing and anti-wrinkle according to claim 1, characterized in that: The fermentation conditions in step (3) are pH 6.0-6.5, aerobic fermentation at 30-35°C for 18-20 hours.

5. The method for preparing camellia seed oil having repairing, soothing and anti-wrinkle properties according to claim 1, characterized in that: The conditions for distilling and collecting the distillate in step (4) are: heating to 102-112° C. to collect the distillate, and the distillation time is 40-50 min.

6. The method for preparing camellia seed oil having the functions of repairing, soothing and anti-wrinkle according to claim 1, characterized in that: In the step (1), camellia seed kernel powder and water are mixed in a weight ratio of 1:(8-10) to obtain a raw material liquid.

7. The method for preparing camellia oil having repairing, soothing and anti-wrinkle properties according to claim 1, characterized in that: In the step (1), the camellia seed kernels are crushed to less than 200 meshes.

8. The method for preparing camellia seed oil having the functions of repairing, soothing and anti-wrinkle according to claim 1, characterized in that: In step (5), 16-18 wt% NaOH aqueous solution is added in an amount of 1.2-1.5 times the theoretical amount of alkali, and the theoretical amount of alkali is equal to 7.13×10 -4 ×crude oil quality×crude oil acid value.

9. Camellia seed oil with repairing, soothing and anti-wrinkle effects obtained by the preparation method according to any one of claims 1 to 8.

10. Use of the camellia seed oil having the repairing, soothing and anti-wrinkle effects as claimed in claim 9 in the preparation of cosmetics.

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