Full-automatic microbe post-treatment blood plate incubation device and method

Abstract

The invention discloses a full-automatic microbe post-treatment blood plate incubation device and method, and relates to the technical field of microbe incubation, the full-automatic microbe post-treatment blood plate incubation device comprises a device main body, a bin door, a display and a heating bin, the bin door is arranged on the front side of the device main body, transparent glass is embedded in the surface of the bin door for observing the interior, and rollers are mounted at the four ends of the bottom of the device main body; according to the scheme, through cooperation of a plurality of mechanisms, the efficient vibration effect on the blood plate is achieved, the vibration can promote mixing of nutrient substances and microorganisms in a culture medium, the utilization rate of the nutrient substances is increased, growth of the microorganisms is accelerated, and meanwhile the effect of improving the quality of the blood plate is achieved. Slight vibration can prevent bacterial colonies from being excessively adhered to the surface of the blood plate, so that the bacterial colonies are more easily observed and analyzed, the experiment accuracy is improved, meanwhile, the phenomenon that the concentration of local nutrient substances on the surface of the blood plate is too high can be reduced, and microorganisms are prevented from excessively growing in a local area.

Description

Technical Field

[0001] The invention relates to the technical field of microbial incubation, and in particular to a fully automatic microbial post-processing blood plate incubation device and method. Background Art

[0002] In the field of microbial culture, blood agar plates are a commonly used experimental tool for culturing and observing the growth of microorganisms. Traditional blood agar plate culture devices usually use simple constant temperature boxes or incubators to culture microorganisms;

[0003] After searching, the Chinese patent publication number CN117778159A discloses a fully automatic microbial post-processing blood plate incubation device and method. Although the blood plate incubation mechanism can not only provide blood plate incubation for the fully automatic microbial post-processing system, it can also be used alone. Providing blood plate rotation and detecting the presence of blood plates can achieve accurate positioning accuracy. At the same time, the plates in the incubator can be photographed at regular intervals, and the blood plate growth process can be observed without taking out the plates, thereby reducing the risk of bacterial transmission; but during the culture process, the nutrients in the culture medium are not fully mixed with the microorganisms, resulting in low utilization of the nutrients. In addition, the colonies tend to excessively adhere to the surface of the blood plate, affecting the observation and analysis of the colonies, thereby reducing the accuracy and reliability of the experiment;

[0004] In addition, existing culture devices usually lack an effective light regulation mechanism, resulting in uneven light exposure in different directions of the blood plate. This uneven light exposure can cause microorganisms to grow too fast or too slow in a local area, resulting in uneven growth of microorganisms on the entire blood plate, affecting the accuracy and consistency of experimental results. Summary of the invention

[0005] In view of the deficiencies of the prior art, the present invention provides a fully automatic microbial post-processing blood plate incubation device and method, which solves the problems of insufficient culture medium mixing and uneven illumination in the prior art mentioned in the background technology.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions: a fully automatic microbial post-processing blood plate incubation device and method, including a device body, a chamber door, a display and a heating chamber, the front of the device body is provided with a chamber door, the surface of the chamber door is embedded with transparent glass for observing the interior, the four ends of the bottom of the device body are installed with rollers, the rollers are used to facilitate the overall movement of the device, the top of the device body is provided with a top plate, the interior of the top plate is embedded with transparent glass, a controller is provided on one side of the device body, the controller is used to control the overall operation of the device, a display is installed on the surface of the controller, a heating chamber is provided at one end of the bottom of the device body, the heating chamber is used to heat the interior of the device to maintain a constant temperature, a heating coil is provided inside the heating chamber, a heat dissipation hole is opened on the top of the heating chamber, and a fixing mechanism is provided above the heating chamber;

[0007] The fixing mechanism is used to place the blood plate, and the fixing mechanism includes an angle adjustment mechanism and a placement mechanism. The placement mechanism is used to limit the position of the blood plate. The angle adjustment mechanism is located on one side of the placement mechanism, and the angle adjustment mechanism is used to adjust the angle of the placement mechanism to facilitate observation;

[0008] A driving mechanism is provided on one side of the fixing mechanism, and the driving mechanism is used to adjust the position of the blood plate inside the device body. The driving mechanism includes a vibration mechanism and a translation mechanism. The vibration mechanism is used to cooperate with the placement mechanism to achieve vibration of the blood plate, and the translation mechanism is used to adjust the position of the vibration mechanism.

[0009] Preferably, the placement mechanism includes a placement plate and a rotating mechanism, one end of the placement plate is rotatably connected to the inner wall of the device body through a rotating shaft, four placement plates are placed from top to bottom, holes are opened on the surface of the placement plate, the holes on the surface of the placement plate are distributed in a straight line with equal spacing, positioning blocks are arranged inside the holes on the surface of the placement plate, the positioning blocks are used to support the blood plate, and the rotating mechanism is located at the bottom of the placement plate. The placement mechanism can effectively fix and evenly distribute the blood plate by setting a plurality of equally spaced placement plates and rotating mechanisms, and at the same time realize all-round rotation of the blood plate, thereby ensuring that the microorganisms receive light and nutrients evenly in different directions, thereby improving the accuracy and consistency of the experiment.

[0010] Preferably, the rotating mechanism includes a fifth motor, a second worm is mounted on the outside of the output end of the fifth motor, four second worm wheels are arranged on one side of the second worm, and the second worm is respectively meshed with the four second worm wheels, one end of the bottom of the second worm wheel is rotatably connected to one end of the bottom of the fixing frame, and the top of the other end of the fixing frame is welded to the bottom of the placement plate. The rotating mechanism utilizes the fifth motor to drive the meshing transmission of the second worm and the second worm wheel to achieve precise rotation of the placement plate, thereby ensuring that the blood plate receives light and nutrients evenly in different directions, thereby improving the uniformity of microbial growth and the accuracy of experimental results.

[0011] Preferably, one end of the top of the second worm gear is welded to the bottom of the telescopic rod, and one end of the top of the telescopic rod is welded to the bottom of the positioning block. A spring is sleeved on the outside of the telescopic rod. Through the design of the telescopic rod and the spring, the flexibility and stability of the positioning block are enhanced, and the vibration energy can be effectively absorbed and buffered during the vibration of the blood plate, preventing the blood plate from being displaced or damaged due to excessive vibration, thereby ensuring the stable fixation of the blood plate and the smooth progress of the experiment.

[0012] Preferably, the angle adjustment mechanism includes a fourth motor, the fourth motor is mounted on the inner wall of the device body, a first worm is rotatably mounted on the outside of the bottom output end of the fourth motor, four first worm wheels are arranged on one side of the first worm, the first worm wheel is meshed with the surface of the first worm, one side of the first worm wheel is rotatably connected to the inner wall of the device body, and the other side of the first worm wheel is welded to one end of the placement plate. The angle adjustment mechanism drives the meshing transmission of the first worm and the first worm wheel through the fourth motor to achieve precise angle adjustment of the placement plate, which is convenient for experimenters to intuitively observe the growth of microorganisms on the surface of the blood plate, avoid frequent opening of the device body, effectively prevent external contamination, and ensure the sterile environment of the experiment and the accuracy of the results.

[0013] Preferably, the translation mechanism includes a first motor, a first threaded rod is mounted on the outside of the output end of the first motor, the outside of the first threaded rod is threadedly connected to the bottom of the base, both ends of the bottom of the base are in sliding contact with the outer wall of the sliding rod, both ends of the sliding rod are respectively welded to the inner wall of the device body, and the base is in a U-shaped structure. The translation mechanism drives the first threaded rod and the base through the first motor to achieve precise translation of the base, and cooperates with the sliding support of the sliding rod to ensure flexible adjustment of the position of the blood plate inside the device, thereby improving the convenience of experimental operation.

[0014] Preferably, the two sides of the top of the base are welded to one side of the horizontal plate, and a second motor is installed at one end of the horizontal plate by bolts, and a transmission wheel is mounted on the outside of the output end of the second motor, and meshing teeth are distributed on the surface of the transmission wheel. Two transmission wheels are provided, and the two transmission wheels are respectively meshed with two ends of the transmission belt, and the transmission belt is connected to the outside of the vertical plate, and a groove is provided on the surface of one side of the vertical plate, and the groove is slidably connected to two first guide rails fixed on the surface of the horizontal plate, and a third motor is installed at one end of the top of the vertical plate, and a third threaded rod is mounted on the outside of the output end of the third motor, and the outside of the third threaded rod is threadedly connected to one side of the lifting block, and one side of the lifting block is slidably connected to the two second guide rails on the surface of the vertical plate.

[0015] Preferably, the vibration mechanism includes a cylinder, the bottom of which is embedded in one end of the bottom of the lifting block, a support plate is welded to the telescopic end of the bottom of the cylinder, four limit rods are welded to the surface of one end of the support plate, and the outer wall of the limit rod is slidably connected to the inside of the lifting block. The driving mechanism drives the transmission wheel and the transmission belt through the second motor to achieve horizontal movement of the vertical plate, and the third motor drives the third threaded rod to be threadedly connected to the lifting block to achieve vertical movement of the lifting block, and cooperates with the sliding support of the guide rail to ensure the precise positioning of the support plate and the stable movement of the blood plate, thereby improving the accuracy of experimental operations.

[0016] Preferably, the method steps are as follows:

[0017] S01. Open the door of the incubation device, place the blood plates containing the target microorganisms in the grooves of the plates in turn, and ensure that the blood plates are stably fixed; then close the door, start the heating chamber, and maintain a constant temperature inside the device through a precise temperature control system to provide suitable environmental conditions for the growth of microorganisms;

[0018] S02. The driving mechanism is started, and the second motor drives the transmission wheel to rotate. The transmission wheel pulls the vertical plate to move in the horizontal direction through the transmission belt to achieve the lateral horizontal displacement of the support plate; at the same time, the third motor drives the third threaded rod to rotate, and the lifting block moves up and down in the vertical direction through the thread transmission to complete the height adjustment of the support plate; in addition, the first motor drives the first threaded rod to rotate, and the thread transmission causes the base to move forward and backward along the moving direction of the support plate, and finally the support plate is accurately positioned above the blood plate;

[0019] S03. The telescopic end of the cylinder extends downward, and the bottom of the cylinder pushes the support plate downward to fit closely to the raised edge of the top of the blood plate; at this time, the spring and the telescopic rod at the bottom of the positioning block are compressed under the pressure; when the telescopic end of the cylinder contracts upward rapidly, the spring rebounds quickly, pushing the top cover of the blood plate and the blood plate as a whole to quickly reset upward, and with the help of the reciprocating rapid telescopic movement of the telescopic end of the cylinder, the blood plate is efficiently vibrated; this vibration process helps to break the static interface between the culture medium and the microorganisms, promote the full mixing of nutrients in the culture medium and microorganisms, improve the absorption efficiency of nutrients, and accelerate the metabolic activities and growth and reproduction of microorganisms; at the same time, slight vibration can also effectively prevent excessive adhesion and aggregation of bacteria on the surface of the blood plate, making the distribution of bacteria more uniform, facilitating subsequent observation and analysis, thereby improving the accuracy and reliability of experimental results;

[0020] S04. After the driving mechanism drives the support plate to reset, the fifth motor starts and drives the second worm to rotate; the second worm and the second worm gear are meshed and driven to rotate the second worm gear; the second worm gear is connected to the positioning block through the telescopic rod, driving the positioning block to rotate synchronously; the rotation of the positioning block then drives the blood plate carried on the top of it to rotate in all directions; the rotation of the blood plate ensures that the microorganisms receive light evenly in different directions, avoiding local growth that is too fast or too slow due to differences in light intensity or angle, so that the growth of microorganisms on the entire blood plate is more balanced; for the blood plate in the liquid culture medium, the rotation can also promote the full mixing of the culture medium and the microorganisms, so that the nutrients are more evenly distributed on the blood plate, which helps the microorganisms obtain the required nutrients more efficiently, further improves the utilization rate of nutrients, and accelerates the growth rate of microorganisms;

[0021] S05. When the positioning block stops rotating, the grooves on its surface are precisely aligned with the grooves on the surface of the placement plate; the fourth motor starts to drive the first worm to rotate; the first worm is meshed with the four first worm gears to make the four first worm gears rotate synchronously; the first worm gear is connected to the placement plate to drive the placement plate to adjust the angle and achieve tilting; the tilting of the placement plate is conducive to the experimenter to directly observe the growth of microorganisms on the surface of the blood plate through the door, avoiding frequent opening of the door for observation, thereby effectively preventing pollutants in the external air from entering the main body of the device, ensuring the sterile environment of the blood plate, and ensuring the accuracy and reliability of the experiment;

[0022] S06. When the placement plate is reset to a horizontal angle, the drive mechanism starts again to drive the support plate to move; one end of the support plate is accurately inserted into the groove on the surface of the placement plate and tightly combined with the groove on the surface of the positioning block; then, the drive mechanism continues to work, driving the support plate to lift up, holding the bottom of the blood plate for movement; by adjusting the position of the blood plate inside the main body of the device, the blood plate is prevented from being in the same position for a long time, reducing the uneven growth of microorganisms caused by slight differences in local environmental conditions (such as temperature and humidity); constantly adjusting the position of the blood plate so that the microorganisms grow alternately under the environmental conditions of different positions, which helps to improve the uniformity of microbial growth on the entire blood plate and ensure the stability and consistency of the experimental results.

[0023] The present invention provides a fully automatic microbial post-processing blood plate incubation device and method. It has the following beneficial effects:

[0024] (1) The fully automatic microbial post-processing blood plate incubation device and method, through the coordinated cooperation of multiple mechanisms, achieves an efficient vibration effect on the blood plate. This vibration can promote the mixing of nutrients in the culture medium and microorganisms, improve the utilization rate of nutrients, and accelerate the growth of microorganisms. At the same time, slight vibration can also prevent excessive adhesion of colonies on the surface of the blood plate, making the colonies easier to observe and analyze, thereby improving the accuracy of the experiment. At the same time, it can also reduce the phenomenon of excessive local nutrient concentration on the surface of the blood plate, thereby avoiding excessive growth of microorganisms in local areas, resulting in uneven growth. This uniform growth environment helps to obtain more consistent experimental results.

[0025] (2) The fully automatic microbial post-processing blood plate incubation device and method drives the second worm and the second worm gear to rotate through the fifth motor, thereby enabling the blood plate to rotate. The rotation of the blood plate allows the microorganisms to receive light evenly in different directions, avoiding local growth that is too fast or too slow due to uneven light, and making the growth of microorganisms on the entire blood plate more uniform. In addition, in the liquid culture medium, the rotation of the blood plate can also promote the mixing of the culture medium and the microorganisms, making the nutrients more evenly distributed, helping the microorganisms to obtain the required nutrients more quickly, improving the utilization rate of nutrients, and thus accelerating the growth rate of the microorganisms.

[0026] (3) The fully automatic microbial post-processing blood plate incubation device and method starts the fourth motor to drive the first worm to rotate; the first worm is meshed with the four first worm wheels to make the four first worm wheels rotate synchronously; the first worm wheel is connected to the placement plate to drive the placement plate to adjust the angle and achieve tilting; the tilting of the placement plate is conducive to the experimenter to directly observe the growth of microorganisms on the surface of the blood plate through the chamber door, avoiding frequent opening of the chamber door for observation, thereby effectively preventing pollutants in the external air from entering the interior of the device body, ensuring the sterile environment of the blood plate, and ensuring the accuracy and reliability of the experiment.

[0027] Thereby, the problems of insufficient culture medium mixing and uneven illumination in the prior art are solved. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0029] Figure 2 It is a schematic diagram of the internal structure of the present invention;

[0030] Figure 3 It is a schematic diagram of the structure of the driving mechanism of the present invention;

[0031] Figure 4 It is a schematic diagram of the structure of the first worm wheel and the first worm of the present invention;

[0032] Figure 5 This is a schematic diagram of the positioning block structure of the present invention;

[0033] Figure 6 is a schematic diagram of the second worm structure of the present invention;

[0034] Figure 7 It is a schematic diagram of the telescopic rod structure of the present invention;

[0035] Figure 8 It is a schematic diagram of the structure of the driving mechanism of the present invention;

[0036] Fig. 9 For the present invention Figure 8 Enlarged structural diagram at A in the middle.

[0037] In the figure, 1, device body; 2, door; 3, roller; 4, top plate; 5, controller; 6, display; 7, driving mechanism; 701, base; 702, slide bar; 703, first motor; 704, first threaded rod; 705, horizontal plate; 706, second motor; 707, transmission wheel; 708, transmission belt; 709, first guide rail; 710, vertical plate; 711, third motor; 712, second guide rail; 713, third Three threaded rods; 714, lifting block; 715, cylinder; 716, support plate; 717, limit rod; 8, fixing mechanism; 801, first worm gear; 802, second worm; 803, fourth motor; 804, placement plate; 805, positioning block; 806, rotating shaft; 807, fifth motor; 808, second worm; 809, second worm gear; 810, fixing frame; 811, spring; 812, telescopic rod; 9, heating chamber. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] Embodiment 1:

[0040] See also Figure 1-9The embodiment of the present invention provides a technical solution: a fully automatic microbial post-processing blood plate incubation device and method, including a device body 1, a chamber door 2, a display 6 and a heating chamber 9. The front of the device body 1 is provided with a chamber door 2, and the surface of the chamber door 2 is embedded with transparent glass for observing the interior. Rollers 3 are installed at the four ends of the bottom of the device body 1, and the rollers 3 are used to facilitate the overall movement of the device. A top plate 4 is provided on the top of the device body 1, and transparent glass is embedded inside the top plate 4. A controller 5 is provided on one side of the device body 1, and the controller 5 is used for overall control of the device. A display 6 is installed on the surface of the controller 5, and a heating chamber 9 is provided at one end of the bottom of the device body 1. A heat chamber 9 is used to heat the inside of the device to maintain a constant temperature. A heating coil is arranged inside the heating chamber 9. A heat dissipation hole is opened on the top of the heating chamber 9. A fixing mechanism 8 is arranged above the heating chamber 9. The fixing mechanism 8 is used to place a blood plate. The fixing mechanism 8 includes an angle adjustment mechanism and a placement mechanism. The placement mechanism is used to limit the blood plate. The angle adjustment mechanism is located on one side of the placement mechanism. The angle adjustment mechanism is used to adjust the angle of the placement mechanism for easy observation. A driving mechanism 7 is arranged on one side of the fixing mechanism 8. The driving mechanism 7 is used to adjust the position of the blood plate inside the device body 1. The driving mechanism 7 includes a vibration mechanism and a translation mechanism. The vibration mechanism is used to cooperate with the placement mechanism to realize the vibration of the blood plate, and the translation mechanism is used to adjust the position of the vibration mechanism; the translation mechanism includes a first motor 703, and the first motor 703 output end is externally sleeved with a first threaded rod 704, the first threaded rod 704 is externally threadedly connected to the bottom of the base 701, and the two ends of the bottom of the base 701 are in sliding contact with the outer wall of the slide rod 702, and the two ends of the slide rod 702 are respectively welded to the inner wall of the device body 1, and the base 701 is in a U-shaped structure; the two sides of the top of the base 701 are welded to one side of the horizontal plate 705, and the second motor 706 is installed at one end of the horizontal plate 705 by bolts, and the output end of the second motor 706 is externally sleeved with a transmission Wheel 707, gear teeth are distributed on the surface of the transmission wheel 707, two transmission wheels 707 are provided, the two transmission wheels 707 are respectively meshed with the two ends of the transmission belt 708, the transmission belt 708 is connected to the vertical plate 710 outside, a groove is provided on the surface of one side of the vertical plate 710, and the groove is slidably connected with two first guide rails 709 fixed on the surface of the horizontal plate 705, a third motor 711 is installed at one end of the top of the vertical plate 710, a third threaded rod 713 is externally sleeved on the output end of the third motor 711, the third threaded rod 713 is externally threadedly connected with one side of the lifting block 714, and one side of the lifting block 714 is slidably connected with two second guide rails 712 on the surface of the vertical plate 710;The vibration mechanism includes a cylinder 715, the bottom of which is embedded in one end of the bottom of the lifting block 714, a support plate 716 is welded to the telescopic end of the bottom of the cylinder 715, and four limit rods 717 are welded to the surface of one end of the support plate 716. The outer wall of the limit rod 717 is slidably connected with the inside of the lifting block 714, and the driving mechanism 7 is started. The second motor 706 drives the transmission wheel 707 to rotate, and the transmission wheel 707 pulls the vertical plate 710 to move in the horizontal direction through the transmission belt 708, so as to realize the lateral horizontal displacement of the support plate 716; at the same time, the third motor 711 drives the third threaded rod 713 to rotate, and the lifting block 714 moves up and down in the vertical direction through the threaded transmission, so as to complete the height adjustment of the support plate 716; in addition, the first motor 703 drives the first threaded rod 704 to rotate, and the threaded transmission causes the base 701 to move forward and backward along the moving direction of the support plate 716, and finally accurately positions the support plate 716 just above the blood plate; the telescopic end of the cylinder 715 extends downward, and the cylinder 715 The bottom pushes the support plate 716 downward to fit closely to the raised edge of the top of the blood plate; at this time, the spring 811 and the telescopic rod 812 at the bottom of the positioning block 805 are compressed under pressure; when the telescopic end of the cylinder 715 shrinks upward rapidly, the spring 811 rebounds quickly, pushing the top cover of the blood plate and the entire blood plate to quickly return upward, and the blood plate is efficiently vibrated by means of the reciprocating rapid telescopic movement of the telescopic end of the cylinder 715; through the coordinated cooperation of multiple mechanisms, the efficient vibration effect of the blood plate is achieved, which can promote the mixing of nutrients and microorganisms in the culture medium, improve the utilization rate of nutrients, and accelerate the growth of microorganisms. At the same time, slight vibration can also prevent excessive adhesion of bacteria on the surface of the blood plate, making the bacteria easier to observe and analyze, thereby improving the accuracy of the experiment, and at the same time, it can also reduce the phenomenon of excessive concentration of local nutrients on the surface of the blood plate, thereby avoiding excessive growth of microorganisms in local areas, resulting in uneven growth. ;

[0041] Embodiment 2:

[0042] See also Figure 1-7, the embodiment of the present invention provides a technical solution: a fully automatic microbial post-processing blood plate incubation device and method, the placement mechanism includes a placement plate 804 and a rotating mechanism, one end of the placement plate 804 is rotatably connected to the inner wall of the device body 1 through a rotating shaft 806, and there are four placement plates 804 placed from top to bottom, and holes are opened on the surface of the placement plate 804. The holes and grooves on the surface of the placement plate 804 are distributed in a straight line with equal spacing. Positioning blocks 805 are arranged inside the holes and grooves on the surface of the placement plate 804. The positioning blocks 805 are used to support The blood plate, the rotating mechanism is located at the bottom of the placement plate 804, the rotating mechanism includes a fifth motor 807, the output end of the fifth motor 807 is externally sleeved with a second worm 808, one side of the second worm 808 is provided with four second worm wheels 809, and the second worm 808 is respectively engaged with the four second worm wheels 809, one end of the bottom of the second worm wheel 809 is rotatably connected to one end of the bottom of the fixing frame 810, and the top of the other end of the fixing frame 810 is welded to the bottom of the placement plate 804; one end of the top of the second worm wheel 809 is connected to the telescopic rod 812 The bottom is welded, and one end of the top of the telescopic rod 812 is welded to the bottom of the positioning block 805. A spring 811 is sleeved on the outside of the telescopic rod 812. After the driving mechanism 7 drives the support plate 716 to reset, the fifth motor 807 is started to drive the second worm 808 to rotate; the second worm 808 is meshed with the second worm wheel 809 to rotate; the second worm wheel 809 is connected to the positioning block 805 through the telescopic rod 812, driving the positioning block 805 to rotate synchronously; the rotation of the positioning block 805 drives the blood plate carried on the top of it to rotate in all directions; the rotation of the blood plate ensures that the microorganisms receive light uniformly in different directions, avoids local growth that is too fast or too slow due to differences in light intensity or angle, and makes the growth of microorganisms on the entire blood plate more balanced; for the blood plate in the liquid culture medium, the rotation can also promote the full mixing of the culture medium and the microorganisms, so that the nutrients are more evenly distributed on the blood plate, which helps the microorganisms to obtain the required nutrients more efficiently, further improves the utilization rate of nutrients, and accelerates the growth rate of microorganisms.

[0043] Embodiment 3:

[0044] See also Figure 1-4, the embodiment of the present invention provides a technical solution: a fully automatic microbial post-processing blood plate incubation device and method, the angle adjustment mechanism includes a fourth motor 803, the fourth motor 803 is installed on the inner wall of the device body 1, the fourth motor 803 bottom output end is sleeved with a first worm 802, one side of the first worm 802 is provided with four first worm wheels 801, the first worm wheel 801 is meshed with the surface of the first worm 802, one side of the first worm wheel 801 is rotatably connected to the inner wall of the device body 1, the other side of the first worm wheel 801 is welded to one end of the placement plate 804, the fourth motor 803 Start, drive the first worm 802 to rotate; the first worm 802 is meshed with the four first worm wheels 801 for transmission, so that the four first worm wheels 801 rotate synchronously; the first worm wheel 801 is connected to the placement plate 804, driving the placement plate 804 to adjust the angle to achieve tilting; the tilting of the placement plate 804 is conducive to the experimenter to directly observe the growth of microorganisms on the surface of the blood plate through the chamber door 2, avoid frequent opening of the chamber door 2 for observation, thereby effectively preventing pollutants in the external air from entering the interior of the device body 1, ensuring the sterile environment of the blood plate, and ensuring the accuracy and reliability of the experiment.

[0045] Embodiment 4:

[0046] See also Figure 1-9 The embodiment of the present invention provides a technical solution: a fully automatic microbial post-processing blood plate incubation device and method, the method steps are as follows:

[0047] S01. By opening the cabinet door, the blood plate containing microorganisms is placed in the groove on the surface of the placement plate 804, so that the groove on the surface of the placement plate 804 is placed in turn on the blood plate, and then the temperature inside the device body 1 is maintained by the heating chamber 9, so that the temperature inside the heating chamber 9 remains stable;

[0048] S02. The second motor 706 of the driving mechanism 7 drives the transmission wheel 707 to rotate, so that the transmission wheel 707 can drive the vertical plate 710 to move through the transmission belt 708, so that the support plate 716 can move horizontally, and then the third motor 711 drives the third threaded rod 713 to rotate, so that the third threaded rod 713 can drive the lifting block 714 to move up and down, so that the support plate 716 can be adjusted up and down, and then the first motor 703 drives the first threaded rod 704 to rotate, and the first threaded rod 704 can drive the base 701 to move while rotating, so that the base 701 can move the support plate 716 back and forth, so that the support plate 716 can move above the blood plate;

[0049] S03. Then, the telescopic end of the cylinder 715 extends downward, so that the bottom of the cylinder 715 can drive the support plate 716 to press down the top cover of the blood plate. At the same time, the spring 811 and the telescopic rod 812 at the bottom of the positioning block 805 will shrink under pressure. When the telescopic end of the cylinder 715 shrinks upward, the blood plate will quickly reset upward under the push of the spring 811. The telescopic end of the cylinder 715 quickly retracts to achieve a vibration effect on the blood plate; vibration can promote the mixing of nutrients and microorganisms in the culture medium, improve the utilization rate of nutrients, and accelerate the growth of microorganisms. Slight vibration can prevent excessive adhesion of bacteria on the surface of the blood plate, making the bacteria easier to observe and analyze; improve the accuracy of the experiment;

[0050] S04. After the driving mechanism 7 drives the support plate 716 to reset, the fifth motor 807 drives the second worm 808 to rotate. The second worm 808 can drive the second worm wheel 809 to rotate while rotating, so that the second worm wheel 809 can drive the positioning block 805 to rotate through the telescopic rod 812. The positioning block 805 can also drive the blood plate on the top to rotate while rotating. The blood plate rotation can make the microorganisms receive light evenly in different directions, avoiding local growth too fast or too slow due to uneven light, making the growth of microorganisms on the entire blood plate more even. In the liquid culture medium, the blood plate rotation can promote the mixing of the culture medium and microorganisms, making the nutrients more evenly distributed. This helps the microorganisms obtain the required nutrients more quickly, improves the utilization rate of nutrients, and thus accelerates the growth rate of microorganisms;

[0051] S05. When the positioning block 805 stops rotating, the groove on the surface of the positioning block 805 will be aligned with the groove on the surface of the placement plate 804, and then the first worm 802 will be driven to rotate by the fourth motor 803. The first worm 802 can drive the four first worm wheels 801 to rotate at the same time, so that the four first worm wheels 801 can drive the four placement plates 804 to rotate, so that the placement plates 804 can be adjusted in angle, so as to be tilted, which is conducive to observing the internal blood plate surface through the chamber door 2, thereby avoiding opening the chamber door 2 for observation, and preventing external air from entering the device body 1 to contaminate the blood plate;

[0052] S06. When the placement plate 804 is reset to a horizontal angle, the support plate 716 is driven to move by the driving mechanism 7, so that one end of the support plate 716 can be inserted into the groove on the surface of the positioning block 805 through the groove on the surface of the placement plate 804, and then the support plate 716 is lifted up by the driving mechanism 7, so that the support plate 716 can support the bottom of the blood plate for movement, thereby adjusting the position of the blood plate inside the device body 1, avoiding the blood plate from being in the same position for a long time, resulting in uneven growth of microorganisms due to slight differences in local environmental conditions. By continuously adjusting the position of the blood plate, the microorganisms can be alternately grown under the environmental conditions of different positions, reducing the phenomenon of local uneven growth, and improving the uniformity of microbial growth on the entire blood plate.

[0053] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0054] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. Fully automatic microbial post-processing blood plate incubation device, characterized by: The device comprises a device body (1), a door (2), a display (6) and a heating chamber (9); the device body (1) is provided with a door (2) on the front, and a transparent glass is embedded on the surface of the door (2) for observing the interior; rollers (3) are installed at the four ends of the bottom of the device body (1), and the rollers (3) are used to facilitate the overall movement of the device; a top plate (4) is provided on the top of the device body (1), and transparent glass is embedded inside the top plate (4); a controller (5) is provided on one side of the device body (1), and the controller (5) is used to control the overall operation of the device; a display (6) is installed on the surface of the controller (5); a heating chamber (9) is provided at one end of the bottom of the device body (1), and the heating chamber (9) is used to heat the interior of the device to maintain a constant temperature; a heating coil is provided inside the heating chamber (9), and a heat dissipation hole is opened on the top of the heating chamber (9); a fixing mechanism (8) is provided above the heating chamber (9); The fixing mechanism (8) is used to place the blood plate, and the fixing mechanism (8) comprises an angle adjustment mechanism and a placement mechanism. The placement mechanism is used to limit the position of the blood plate, and the angle adjustment mechanism is located on one side of the placement mechanism. The angle adjustment mechanism is used to adjust the angle of the placement mechanism to facilitate observation; A driving mechanism (7) is provided on one side of the fixing mechanism (8), and the driving mechanism (7) is used to adjust the position of the blood plate inside the device body (1). The driving mechanism (7) comprises a vibration mechanism and a translation mechanism. The vibration mechanism is used to cooperate with the placement mechanism to achieve vibration of the blood plate, and the translation mechanism is used to adjust the position of the vibration mechanism.

2. The fully automatic microbial post-processing blood plate incubation device according to claim 1, characterized in that: The placement mechanism comprises a placement plate (804) and a rotating mechanism. One end of the placement plate (804) is rotatably connected to the inner wall of the device body (1) via a rotating shaft (806). Four placement plates (804) are placed from top to bottom. The surface of the placement plate (804) is provided with holes and grooves. The holes and grooves on the surface of the placement plate (804) are distributed in a straight line with equal spacing. Positioning blocks (805) are arranged inside the holes and grooves on the surface of the placement plate (804). The positioning blocks (805) are used to support the blood plate. The rotating mechanism is located at the bottom of the placement plate (804).

3. The fully automatic microbial post-processing blood plate incubation device according to claim 2, characterized in that: The rotating mechanism comprises a fifth motor (807), the output end of the fifth motor (807) is externally sleeved with a second worm (808), one side of the second worm (808) is provided with four second worm wheels (809), and the second worm (808) is respectively meshed with the four second worm wheels (809), one end of the bottom of the second worm wheel (809) is rotatably connected to one end of the bottom of a fixing frame (810), and the top of the other end of the fixing frame (810) is welded to the bottom of the placement plate (804).

4. The fully automatic microbial post-processing blood plate incubation device according to claim 3, characterized in that: One end of the top of the second worm gear (809) is welded to the bottom of the telescopic rod (812), one end of the top of the telescopic rod (812) is welded to the bottom of the positioning block (805), and a spring (811) is sleeved on the outside of the telescopic rod (812).

5. The fully automatic microbial post-processing blood plate incubation device according to claim 1, characterized in that: The angle adjustment mechanism comprises a fourth motor (803), the fourth motor (803) is mounted on the inner wall of the device body (1), a first worm (802) is sleeved on the outside of the bottom output end of the fourth motor (803), four first worm wheels (801) are arranged on one side of the first worm (802), the first worm wheels (801) are meshed with the surface of the first worm (802), one side of the first worm wheel (801) is rotatably connected to the inner wall of the device body (1), and the other side of the first worm wheel (801) is welded to one end of the placement plate (804).

6. The fully automatic microbial post-processing blood plate incubation device according to claim 1, characterized in that: The translation mechanism comprises a first motor (703), the output end of the first motor (703) is externally sleeved with a first threaded rod (704), the first threaded rod (704) is externally threadedly connected to the bottom of the base (701), the two ends of the bottom of the base (701) are in sliding contact with the outer wall of the sliding rod (702), the two ends of the sliding rod (702) are respectively welded to the inner wall of the device body (1), and the base (701) is in a U-shaped structure.

7. The fully automatic microbial post-processing blood plate incubation device according to claim 1, characterized in that: The top two sides of the base (701) are welded to one side of the horizontal plate (705); a second motor (706) is installed on one end of the horizontal plate (705) by bolts; a transmission wheel (707) is mounted on the outside of the output end of the second motor (706); a surface of the transmission wheel (707) is provided with meshing teeth; two transmission wheels (707) are provided; the two transmission wheels (707) are respectively meshed with two ends of a transmission belt (708); and the transmission belt (708) is connected to an external vertical plate (710); A groove is provided on one side surface of the vertical plate (710), and the groove is slidably connected to two first guide rails (709) fixed on the surface of the horizontal plate (705); a third motor (711) is installed on one end of the top of the vertical plate (710); a third threaded rod (713) is sleeved on the outside of the output end of the third motor (711); the outside of the third threaded rod (713) is threadedly connected to one side of the lifting block (714); and one side of the lifting block (714) is slidably connected to two second guide rails (712) on the surface of the vertical plate (710).

8. The fully automatic microbial post-processing blood plate incubation device according to claim 1, characterized in that: The vibration mechanism comprises a cylinder (715), the bottom of the cylinder (715) is embedded in one end of the bottom of the lifting block (714), a support plate (716) is welded to the telescopic end of the bottom of the cylinder (715), four limit rods (717) are welded to the surface of one end of the support plate (716), and the outer wall of the limit rod (717) is slidably connected to the inside of the lifting block (714).

9. The fully automatic microbial post-processing blood plate incubation method according to any one of claims 1 to 8, characterized in that: The method steps are as follows: S01. Open the door of the incubation device, and place the blood plates containing the target microorganisms in the grooves of the placement plate (804) in turn to ensure that the blood plates are stably fixed; then close the door, start the heating chamber (9), and maintain a constant temperature inside the device through a precise temperature control system to provide suitable environmental conditions for the growth of microorganisms; S02. The driving mechanism (7) is started, and the second motor (706) is operated to drive the transmission wheel (707) to rotate. The transmission wheel (707) pulls the vertical plate (710) to move in the horizontal direction through the transmission belt (708), thereby realizing the lateral horizontal displacement of the support plate (716); at the same time, the third motor (711) drives the third threaded rod (713) to rotate, and the lifting block (714) is moved up and down in the vertical direction through the threaded transmission, thereby completing the height adjustment of the support plate (716); in addition, the first motor (703) drives the first threaded rod (704) to rotate, and the threaded transmission causes the base (701) to move forward and backward along the moving direction of the support plate (716), and finally the support plate (716) is accurately positioned just above the blood plate; S03. The telescopic end of the cylinder (715) extends downward, and the bottom of the cylinder (715) pushes the support plate (716) downward to fit closely to the raised edge of the top of the blood plate; at this time, the spring (811) and the telescopic rod (812) at the bottom of the positioning block (805) are compressed under the pressure; when the telescopic end of the cylinder (715) contracts upward rapidly, the spring (811) rebounds rapidly, pushing the top cover of the blood plate and the entire blood plate to quickly return upward, and with the help of the reciprocating rapid telescopic movement of the telescopic end of the cylinder (715), efficient vibration of the blood plate is achieved; The vibration process helps to break the static interface between the culture medium and the microorganisms, promotes the full mixing of nutrients in the culture medium and the microorganisms, improves the absorption efficiency of nutrients, and accelerates the metabolic activities and growth and reproduction of microorganisms; at the same time, slight vibration can also effectively prevent excessive adhesion and aggregation of colonies on the surface of the blood plate, making the colony distribution more uniform, facilitating subsequent observation and analysis, thereby improving the accuracy and reliability of experimental results; S04. After the driving mechanism (7) drives the support plate (716) to reset, the fifth motor (807) starts to drive the second worm (808) to rotate; the second worm (808) and the second worm wheel (809) are meshed and driven to rotate the second worm wheel (809); the second worm wheel (809) is connected to the positioning block (805) through the telescopic rod (812), driving the positioning block (805) to rotate synchronously; the rotation of the positioning block (805) drives the blood plate carried on the top of it to rotate in all directions; the rotation of the blood plate ensures that the microorganisms receive light evenly in different directions, avoiding local growth that is too fast or too slow due to differences in light intensity or angle, so that the growth of microorganisms on the entire blood plate is more balanced; for the blood plate in the liquid culture medium, the rotation can also promote the full mixing of the culture medium and the microorganisms, so that the nutrients are more evenly distributed on the blood plate, which helps the microorganisms to obtain the required nutrients more efficiently, further improves the utilization rate of nutrients, and accelerates the growth rate of microorganisms; S05. When the positioning block (805) stops rotating, the grooves on its surface are precisely aligned with the grooves on the surface of the placement plate (804); the fourth motor (803) is started to drive the first worm (802) to rotate; the first worm (802) is meshed with the four first worm wheels (801) to drive the four first worm wheels (801) to rotate synchronously; the first worm wheel (801) is connected to the placement plate (804) to drive the placement plate (804) to adjust the angle and achieve tilting; the tilting of the placement plate (804) is conducive to the experimenter to visually observe the growth of microorganisms on the surface of the blood plate through the chamber door (2), avoiding frequent opening of the chamber door (2) for observation, thereby effectively preventing pollutants in the external air from entering the interior of the device body (1), ensuring the sterile environment of the blood plate, and ensuring the accuracy and reliability of the experiment; S06. When the placement plate (804) is reset to a horizontal angle, the drive mechanism (7) is started again to drive the support plate (716) to move; one end of the support plate (716) is precisely inserted into the groove on the surface of the placement plate (804) and tightly combined with the groove on the surface of the positioning block (805); then, the drive mechanism (7) continues to work, driving the support plate (716) to lift up, holding the bottom of the blood plate for movement; by adjusting the position of the blood plate inside the device body (1), the blood plate is prevented from being in the same position for a long time, and the uneven growth of microorganisms caused by slight differences in local environmental conditions (such as temperature and humidity) is reduced; the position of the blood plate is continuously adjusted so that the microorganisms grow alternately under the environmental conditions of different positions, which helps to improve the uniformity of microbial growth on the entire blood plate and ensure the stability and consistency of the experimental results.

Images