Compound Chinese herbal medicine fermentation method, fermentation preparation and application of fermentation preparation in preparation of feed additive for improving lactation performance of sows

Abstract

The invention provides a compound Chinese herbal medicine fermentation method, a fermentation preparation and application of the fermentation preparation in preparation of a feed additive for improving the lactation performance of sows, and relates to the technical field of feed additives. The method comprises the following steps: respectively removing impurities from cowherb seed, ricepaperplant pith, uniflower swisscentaury root and ligusticum wallichii, cleaning, naturally air-drying, crushing and sieving to obtain Chinese herbal medicine powder, and uniformly mixing the powder according to a mass ratio to obtain compound Chinese herbal medicine powder; uniformly mixing 74.5-75.5% of compound Chinese herbal medicine powder, 24-25% of soybean meal and 0.4-0.6% of calcium carbonate, and sterilizing at high temperature and high pressure to obtain a Chinese herbal medicine culture medium; the preparation method comprises the following steps: inoculating a microbial agent containing fermented lactobacillus mucilaginosus into a Chinese herbal medicine culture medium, adjusting the water content, putting the Chinese herbal medicine culture medium into a respiratory zone with a one-way breather valve, and carrying out anaerobic fermentation at 35-40 DEG C for 5-9 days. According to the compound Chinese herbal medicine fermentation method provided by the invention, the fermentation mucus lactobacillus is introduced in the preparation process for solid-state fermentation, so that the content of active ingredients in the Chinese herbal medicine can be increased, such as the content of total flavonoids and total polysaccharides is remarkably increased, and the biological activity of the Chinese herbal medicine is enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of sow feed additives, and in particular to a compound Chinese herbal medicine fermentation method and fermentation preparation and application of the compound Chinese herbal medicine in preparing a feed additive for improving sow lactation performance. Background Art

[0002] The growth and development of newborn piglets depends on the nutrients in breast milk, especially the immunoglobulins in colostrum, which can provide passive immune protection for piglets. Therefore, improving the nutritional and immune components in breast milk is crucial for the early growth of piglets. However, the lactation performance of sows is affected by many factors, including animal nutrition, feed health, high-pressure production environment, etc.

[0003] Historically, antibiotics have been widely used in feed to promote animal growth and prevent diseases. However, due to problems with antibiotic residues and resistance, my country has completely banned the use of antibiotics in feed. As an alternative to antibiotics, herbal feed additives have attracted attention because of their natural, residue-free and pleiotropic properties. They are believed to improve animal health and production performance while reducing environmental impact. The preparation of feed additives usually involves mixing the active ingredients with carrier materials to ensure uniform distribution in the feed. These additives can be added directly to the feed or provided to the feed mill in the form of premixes.

[0004] Economic analysis of feed additives is an important step in assessing their market potential. This usually involves a cost-benefit analysis that considers the relationship between the cost of the additive and its improvement in animal production performance. Although some methods have been used to improve the reproductive performance of sows and the growth performance of piglets, there is still room for improvement, especially in the development of more economical, effective and environmentally friendly feed additives. Studies have shown that certain Chinese herbal medicines such as fenugreek and tongcao can improve the lactation performance of sows and the growth performance of piglets. These studies provide a scientific basis for the development of new feed additives. However, traditional single Chinese herbal medicines as feed additives have limitations in improving the lactation performance of sows, such as unclear effects and single mechanisms of action. In addition, the lactogenic effect of a single Chinese herbal medicine may not be as significant as the combined effects of compound Chinese herbal medicines.

[0005] Existing Chinese patent document CN114886030A discloses a compound Chinese herbal feed additive for sows, which is composed of the following raw materials in parts by weight: 54.5-56.5% of Vaccaria segetalis, 17.0-20.0% of Tongcao, 7.0-9.0% of Chuanxiong, and 17.0-19.0% of Lophatherum gracile. It is used in the late pregnancy of sows. After adding 1.0%-5.5% of the compound Chinese herbal feed additive to the basic feed of sows, the sows are fed evenly with stirring until the end of lactation. It is used in sow feed to improve the lactation performance of sows, improve milk quality, and then improve the immunity of piglets and promote growth. Although the compound Chinese herbal feed additive solves the problem of limited lactation effect of a single Chinese herbal medicine, due to the general increase in the price of Chinese herbal medicines in the market, the cost of compound Chinese herbal medicines in the field of feed additives is too high, and thus it cannot meet the requirements of large-scale and intensive animal husbandry development.

[0006] Therefore, it is urgent to develop a compound Chinese herbal feed additive with low cost and good lactation-promoting effect. Summary of the invention

[0007] The technical problem to be solved by the present invention is: in view of the defects of the prior art, a compound Chinese herbal medicine fermentation method and a fermentation preparation and their application in the preparation of a feed additive for improving the lactation performance of sows are provided.

[0008] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0009] In a first aspect, the present invention provides a method for fermenting a compound Chinese herbal medicine, comprising the following steps:

[0010] (1) Preparing a compound Chinese herbal medicine powder: removing impurities from the Chinese herbal medicine raw materials Vaccaria segetalis, Tongcao, Loulus odoratus, and Chuanxiong, washing them, naturally air-drying them, and then crushing and sieving them to obtain the powders of the Chinese herbal medicines. According to the mass ratio of Vaccaria segetalis, Tongcao, Loulus odoratus, and Chuanxiong being 6±0.1:2±0.1:2±0.1:1±0.1, the powders are mixed evenly to obtain the compound Chinese herbal medicine powder;

[0011] (2) Preparing a Chinese herbal medicine culture medium: mixing the following components in weight percentage: 74.5-75.5% of the compound Chinese herbal medicine powder prepared in (1), 24-26% of soybean powder and 0.3-0.6% of calcium carbonate, and sterilizing by high temperature and high pressure;

[0012] (3) Inoculation and fermentation of bacterial agents: Inoculate the bacterial agent containing fermented mucus lactobacillus into the Chinese herbal medicine culture medium prepared in (2), adjust the water content, and place it in a breathing zone with a one-way breathing valve. Perform anaerobic fermentation at 35-40°C for 5-9 days to obtain the Chinese herbal medicine fermentation preparation, which is then dried at low temperature and stored for later use.

[0013] Furthermore, after the impurities are removed and washed in step (1), each Chinese herbal medicine is naturally air-dried until the water content is less than 11%, and then crushed and passed through a 40-mesh sieve to obtain each Chinese herbal medicine powder.

[0014] Furthermore, the sterilization conditions in step (2) are 118-125° C., 0.15 MPa high-pressure steam sterilization for 20-30 min.

[0015] Furthermore, in a preferred embodiment of the present invention, the fermented Lactobacillus mucilaginosus in step (3) is a cell of a strain with a preservation number of CCTCC NO: M20222085 or a progeny strain or a subclone strain thereof. For specific preservation information, refer to the patent application number CN202310344677.6.

[0016] Furthermore, the form of the bacterial preparation containing fermented mucus lactobacillus in step (3) includes but is not limited to: a liquid preparation, a solid bacterial preparation, a paste bacterial preparation, a suspension, an emulsion, a spray or a semi-solid bacterial preparation.

[0017] Furthermore, the bacterial concentration (viable count) of Lactobacillus mucilaginosus fermentation in the bacterial agent is 1×10 7 ~1×10 9 CFU / mL.

[0018] Furthermore, the inoculation amount of the bacterial agent is 3-5% of the weight of the Chinese herbal medicine culture medium.

[0019] Furthermore, in step (3), the water content is adjusted to 30-50%.

[0020] In a second aspect, the present invention provides a fermentation preparation obtained by the above-mentioned compound Chinese herbal medicine fermentation method.

[0021] In a third aspect, the present invention also provides the use of the above fermentation preparation in the preparation of a feed additive for improving the lactation performance of sows.

[0022] Furthermore, the fermentation preparation is added to the sow's basic diet at a dosage of 1.5-2.5%.

[0023] The Chinese herbal medicine components of the compound prescription of the present invention include Vaccaria segetalis, Tongcao, Chuanxiong and Lophatherum gracile, and the medicinal properties and meridians thereof are analyzed as follows:

[0024] 1. As the main component of the compound Chinese herbal medicine of the present invention, Vaccaria segetalis is the dried mature seeds of the Caryophyllaceae plant, which contains saponins, flavonoid glycosides, cyclic peptide compounds, coumarins and other biologically active ingredients. Vaccaria segetalis is bitter in nature and neutral in nature. It enters the liver meridian and stomach meridian. It is often used as a Chinese herbal medicine for lactation. When added alone, it has a tendency to improve the lactation performance of sows, and can promote the proliferation of mammary gland cells in dairy cows and improve their lactation ability.

[0025] 2. Tongcao is the dried stem pith of the Araliaceae plant Tongtu wood. Tongcao is sweet and mild in nature, and cold in nature. It enters the lung and stomach meridians, has the effect of ventilating and promoting lactation, and can increase the secretion of lactation-related hormones.

[0026] 3. Chuanxiong is a cultivated plant of the genus Ligustrum in the family Apiaceae. Its main chemical components include polysaccharides, essential oils, alkaloids, phenolic acids, phenol lactones, etc. Chuanxiong is pungent and warm in nature. It enters the liver, gallbladder, and pericardium meridians. Its volatile oil can improve antioxidant capacity, enhance lactation ability, and improve milk quality.

[0027] 4. Loulu is the dried root of the Asteraceae plant Qizhou Loulu, which contains active substances such as hemiterpenes, triterpenes, sterols, thiophenes, etc. Loulu is bitter and cold in nature, and belongs to the stomach meridian. It has physiological activities such as immune stimulation, emergency protection, anabolism and anti-inflammatory. The ethanol extract of Loulu can promote the secretion of milk protein and the synthesis of milk fat and lactose.

[0028] 5. The compound Chinese herbal medicine selected by the present invention is composed of Vaccaria segetalis, Tongcao, Lophatherum sutchuenensis and Chuanxiong according to the principle of monarch, minister, assistant and envoy. Vaccaria segetalis has the effects of promoting blood circulation and menstruation, promoting lactation and reducing swelling, and promoting diuresis and relieving stranguria. It is used as the main drug of the compound and plays a major therapeutic role; Tongcao has the effects of clearing away heat and promoting diuresis, ventilating and promoting lactation, and Lophatherum sutchuenensis can clear away heat and detoxify, eliminate carbuncle, promote lactation, relax muscles and dredge meridians. These two herbs are used to match the main drug to stimulate the effect of the main drug; Chuanxiong can promote blood circulation and qi, dispel wind and relieve pain, and harmonize various drugs.

[0029] The present invention has the following beneficial effects:

[0030] The compound Chinese herbal medicine fermentation method provided by the present invention optimizes the ratio of Chinese herbal medicines and the fermentation process, so that the effective content of Chinese herbal medicines in the prepared compound Chinese herbal medicine fermentation preparation is 75%, and the cost can be reduced by 25% compared with the unfermented compound Chinese herbal medicine, making it more economical and practical.

[0031] The compound Chinese herbal medicine fermentation preparation prepared by the present invention is based on a specific ratio of Chinese herbal medicines. By introducing fermented mucus lactobacillus for solid-state fermentation, the content of active ingredients in the Chinese herbal medicines is increased, such as the content of total flavonoids and total polysaccharides is significantly increased, and the biological activity of the Chinese herbal medicines is enhanced.

[0032] The compound Chinese herbal fermented preparation prepared by the present invention can significantly increase the lactation amount and milk quality of sows, improve the milk protein rate, milk fat rate, lactose rate and non-fat solid content in colostrum, thereby improving the lactation performance of sows and the nutritional quality of milk. It can further increase the weaning weight of newborn piglets, speed up the production rhythm, and also have a positive impact on the improvement of postpartum inflammation problems of sows and the health and growth and development of piglets.

[0033] The compound Chinese herbal medicine fermentation preparation prepared by the invention can also improve the palatability of Chinese herbal medicines to sows and increase the feed intake of sows during pregnancy and lactation. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 A schematic diagram of a process for fermenting a compound Chinese herbal medicine provided in an embodiment of the present invention.

[0035] Figure 2 The content of flavonoids in the Chinese herbal medicine culture medium (anaerobic fermentation at 37°C for 24 hours) corresponding to different bacterial liquid addition amounts in the embodiments of the present invention. DETAILED DESCRIPTION

[0036] like Figure 1 As shown, the present invention provides a compound Chinese herbal medicine fermentation method, which specifically comprises the following steps:

[0037] (1) Preparing compound Chinese herbal medicine powder: removing impurities and washing the Chinese herbal medicine raw materials Vaccaria segetalis, Tongcao, Loulus odoratus and Chuanxiong, then naturally air-drying each Chinese herbal medicine until the water content is less than 11%, and then crushing and passing through a 40-mesh sieve to obtain each Chinese herbal medicine powder; according to the mass ratio of Vaccaria segetalis, Tongcao, Loulus odoratus and Chuanxiong being 6±0.1:2±0.1:2±0.1:1±0.1, the powders are mixed evenly to obtain the compound Chinese herbal medicine powder.

[0038] (2) Preparation of Chinese herbal medicine culture medium: Mix the following components in weight percentage: 74.5-75.5% of the compound Chinese herbal medicine powder prepared in (1), 24-25% of soybean powder and 0.3-0.6% of calcium carbonate, and sterilize by high-pressure steam sterilization at 118-125° C. and 0.15 MPa for 20-30 min.

[0039] (3) Inoculation and fermentation of bacterial agents: Inoculate the bacterial agent containing fermented Lactobacillus mucilaginosus into the Chinese herbal medicine culture medium prepared in (2), the inoculation amount of the bacterial agent is 3-5% of the weight of the Chinese herbal medicine culture medium, and the bacterial concentration (number of viable bacteria) of fermented Lactobacillus mucilaginosus in the bacterial agent is 1×10 7 ~1×10 9 CFU / mL, and the water content is adjusted to 30-50%; the form of the bacterial agent containing fermented mucus lactobacillus includes but is not limited to: liquid preparation, solid bacterial agent, paste bacterial agent, suspension, emulsion, spray or semi-solid bacterial agent.

[0040] Then, the mixture is placed in a breathing zone with a one-way breathing valve and anaerobically fermented at 35-40°C for 5-9 days to obtain a Chinese herbal medicine fermentation preparation, which is then dried at low temperature and stored for later use. In the preferred embodiment, anaerobically fermented at 37°C for 7 days.

[0041] In step (3) of the method of the present invention, the fermentation process adopts a solid-state fermentation process, and the water content is generally controlled at 40% to 60%. In order to clarify the effect of the fermentation water content on the fermentation preparation of the present invention, tests were conducted on water contents of 30%, 40%, 50%, and 60%. During the test, it was found that when the water content was 60%, a stream of water had accumulated at the bottom of the Chinese herbal medicine culture medium, which exceeded the water adsorption capacity of the Chinese herbal medicine and did not meet the basic condition of solid-state fermentation without free water. Finally, the fermentation water content was determined to be 30 to 50%.

[0042] In order to further clarify the effect of the inoculation amount of the bacterial agent on the fermentation preparation of the present invention, the content of flavonoids in the herbal medicine culture medium was measured when the fermentation temperature was controlled at 37°C for 24 hours of anaerobic fermentation. The changes of total flavonoids with different bacterial liquid addition amounts were as follows: Figure 2 As shown. Figure 2 It can be seen that when the bacterial liquid addition amount is 3%, 4% and 5%, the flavonoids in the fermented Chinese herbal medicine culture medium have higher values, especially at 4%, the flavonoids reach the maximum value. It is preferred to inoculate 3-5% (V / W) fermented mucus Lactobacillus liquid into the Chinese herbal medicine culture medium for fermentation.

[0043] In order to more clearly understand the above-mentioned objects, features and advantages of the present invention, the present invention is further described in detail below in conjunction with specific implementation methods.

[0044] If the specific conditions are not specified in the examples, the experiments were carried out under conventional conditions or conditions recommended by the manufacturer. If the manufacturers of the reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0045] Example 1

[0046] As a preferred embodiment, this embodiment provides a compound Chinese herbal medicine fermentation method, comprising the following steps:

[0047] (1) Preparation of compound Chinese herbal medicine powder: remove impurities from the Chinese herbal medicine raw materials Vaccaria segetalis, Tongcao, Loulus chinensis, and Chuanxiong, wash them, and air-dry them until the water content is less than 11%, grind them through a 40-mesh sieve to obtain powders of the respective Chinese herbal medicines, and mix the powders uniformly according to the mass ratio of Vaccaria segetalis, Tongcao, Loulus chinensis, and Chuanxiong of 6:2:2:1 to obtain the compound Chinese herbal medicine powder.

[0048] (2) Preparation of Chinese herbal medicine culture medium: Mix the following components in weight percentage: 75% of the compound Chinese herbal medicine powder prepared in (1), 24.5% of soybean powder and 0.5% of calcium carbonate, and sterilize by high-pressure steam at 121° C. and 0.15 MPa for 30 min.

[0049] (3) Inoculation and fermentation of bacterial agents: Inoculate the bacterial agent containing fermented mucus lactobacillus into the Chinese herbal medicine culture medium prepared in (2), adjust the water content, and place it in a breathing zone with a one-way breathing valve, anaerobic fermentation to obtain a Chinese herbal medicine fermentation preparation, and low-temperature drying and storage for standby use. As a preferred embodiment, the fermented mucus lactobacillus selected is a cell of a strain with a preservation number of CCTCC NO: M20222085 or its progeny strain or subclone strain. For specific preservation information, refer to the patent application number CN202310344677.6. The strain with a preservation number of CCTCC NO: M 20222085 was deposited on December 28, 2022, and is deposited in the China Center for Type Culture Collection, and the preservation address is: Wuhan University, Wuhan, China. The strain classification characteristics are: Limosilactobacillus fermentum ZC529. After identification, the strain of CCTCC NO: M 20222085 is a Gram-positive bacterium and survives. The bacterial agent is a bacterial liquid preparation containing fermented mucus Lactobacillus, and the bacterial concentration (number of viable bacteria) of fermented mucus Lactobacillus in the bacterial liquid is 1.5×10 7 CFU / mL.

[0050] As a preferred embodiment, in the solid-state fermentation process of this embodiment, the amount of bacterial liquid added is 4%, the fermentation water content should be controlled at 40%, and the fermentation time and temperature are controlled at 37° C. for anaerobic fermentation for 7 days.

[0051] First, the Chinese herbal medicine culture medium was observed and recorded before and after fermentation. It was found that it was yellow before fermentation and brown after fermentation, with a light sour aroma and a strong medicinal fragrance. The texture was loose, no lumps, clear physical structure, and no mold.

[0052] The conventional nutritional components and active ingredients of the compound Chinese herbal medicine before and after fermentation were determined, and the results are shown in Table 1:

[0053] Table 1 Changes of conventional nutrients and active ingredients in compound Chinese herbal medicines before and after fermentation

[0054] project Before fermentation After fermentation Crude Protein <![CDATA[12.03±0.41 b ]]> <![CDATA[13.66±0.36 a ]]> Crude fat 2.52±0.58 2.55±0.41 Crude Fiber <![CDATA[12.09±0.18 b ]]> <![CDATA[13.58±0.19 a ]]> Coarse ash 5.45±0.18 5.34±0.03 Total flavonoids <![CDATA[6.82±0.88 b ]]> <![CDATA[8.42±0.64 a ]]> Total polysaccharides <![CDATA[19.52±1.15 b ]]> <![CDATA[26.2±1.85 a ]]> Total saponins 1.88±0.36 1.76±0.22 Total alkaloids 9.81±0.22 10.34±0.6

[0055] The compound Chinese herbal medicine powder prepared by the method of step (1) of Example 1 (Vaccaria segetalis, Tongcao, Lophatherum gracile and Ligusticum chuanxiong powders are uniformly mixed in a weight ratio of 6:2:2:1) is used as a comparative example.

[0056] In order to verify the feasibility and effectiveness of the scheme of the present invention, the compound Chinese herbal medicine fermentation preparation prepared in Example 1 and the unfermented compound Chinese herbal medicine powder of the comparative example were subjected to the following test.

[0057] This example uses a 2% addition amount as an example for testing, that is, 2% (instead of corn) of the compound Chinese herbal fermented preparation prepared in Example 1 or the unfermented compound Chinese herbal powder of the comparative example is added to the sow's basic diet, mixed evenly and then fed to the sow. The specific test method is as follows:

[0058] The experiment selected 30 Ningxiang sows with similar parity and good body condition 14 days before delivery and randomly divided them into 3 treatment groups, each treatment had 10 replicates, and each replicate (pen) had 1 pig. The control group was fed a basal diet, and the experimental group added a compound Chinese herbal feed additive and a fermented compound Chinese herbal feed additive to the basal diet according to the proportions described in Example 1 and Example 2, respectively. The pre-feeding period was 7 days, and the experimental period was 28 days (7 days before delivery to 21 days after delivery). The basal diet was formulated with reference to the nutritional requirements of fat sows in NRC (2012). The experimental sows were raised in the same pig house, and the replicates were evenly distributed in the pig house. They were free to eat and drink water, and natural ventilation and lateral negative pressure ventilation were combined. Pig manure was cleaned twice a day, and deworming, disinfection, immunization and feeding management were carried out according to routine procedures. The condition of the sows was observed and recorded every day.

[0059] Record the sow's feed intake, birth process, piglet birth weight, birth litter weight, lactation mortality, lactation diarrhea rate, weaning weight, weaning litter weight, and calculate the sow's milk production. On the day of sow farrowing, ear vein blood and colostrum were collected from 6 sows in each group. On the end of the experiment, piglet blood samples were collected from the anterior vena cava. Six pigs were collected from each group, marked with group dates, and stored in a -20℃ refrigerator to measure milk quality and immunological indicators.

[0060] The sow basal diet group was used as the blank control group. After the compound Chinese herbal medicine fermentation preparation feed additive prepared in Example 1 and the compound Chinese herbal medicine prepared in the comparative example were fed to sows, the effects on the reproductive performance of sows, the feed intake of sows, the production performance of piglets, the composition of sow colostrum, and the serum immune indexes of sows and piglets were shown in Tables 2-6. The same data in each table with different letters indicates significant differences (P<0.05), and the same letters or no letters indicate no significant differences (P>0.05).

[0061] Table 2 Effects of fermented and unfermented feed additives on reproductive performance of sows

[0062]

[0063]

[0064] Feeding sows with compound Chinese herbal powder can increase the weight loss of sows and reduce the number of weak piglets. It can increase the litter weight and average weight of newborns and increase the lactation of sows. Feeding sows with the compound Chinese herbal fermentation preparation feed additive provided by the present invention can retain the positive effects of the compound Chinese herbal powder and increase the number of healthy piglets compared to feeding sows with unfermented compound Chinese herbal powder feed additive. This result shows that adding 2% of the compound Chinese herbal fermentation preparation feed additive to the sow's diet is beneficial to improving the reproductive performance and lactation performance of sows.

[0065] Table 3 Effects of fermented and unfermented feed additives on sow feed intake

[0066]

[0067] As shown in Table 4 above, the use of the compound Chinese herbal fermentation preparation provided by the present invention to feed sows can significantly increase the feed intake of sows during pregnancy and lactation compared to the unfermented compound Chinese herbal powder feed additive. This result shows that the compound Chinese herbal medicine can improve its sensory properties after fermentation, thereby improving the palatability of the sow to the feed; at the same time, the feed intake of the sow in the first week of lactation is significantly increased, indicating that the compound Chinese herbal fermentation preparation feed additive provided by the present invention also has the effect of improving the appetite of sows after delivery and promoting the recovery of the sow's postpartum production state.

[0068] Table 4 Effects of fermented and unfermented feed additives on piglet production performance

[0069]

[0070] As shown in Table 4 above, the compound Chinese herbal fermentation preparation feed additive provided by the present invention can significantly increase the weaning litter weight and the average weight of weaned piglets compared to the unfermented compound Chinese herbal powder, and also has more positive effects on the survival rate during lactation. This shows that the compound Chinese herbal fermentation preparation feed additive provided by the present invention can improve the lactation ability of sows, and the increase in the number of healthy piglets during delivery is conducive to improving the survival rate of piglets during lactation.

[0071] Table 5 Effects of fermented and unfermented feed additives on sow colostrum composition

[0072]

[0073] It can be seen from Table 5 above that the compound Chinese herbal fermentation preparation feed additive provided by the present invention can improve the milk fat rate, milk protein rate and urea nitrogen content of sow colostrum compared with the unfermented compound Chinese herbal powder, indicating that the compound Chinese herbal fermentation preparation feed additive of the present invention can improve the composition of sow colostrum and also has the effect of reducing the postpartum inflammatory response of sows.

[0074] Table 6 Effects of fermented and unfermented feed additives on serum immune parameters of sows and piglets

[0075]

[0076]

[0077] As shown in Table 6 above, the compound Chinese herbal fermentation preparation feed additive provided by the present invention can improve sow serum immunoglobulin A, M, and G as a whole compared with the unfermented compound Chinese herbal powder. The unfermented compound Chinese herbal additive only improves sow serum immunoglobulin G. At the same time, the compound Chinese herbal fermentation preparation feed additive provided by the present invention also has a reducing effect on sow serum inflammatory factors 1 and 6. This shows that the compound Chinese herbal fermentation preparation of the present invention can improve the immunity of sows by increasing the content of sow serum immunoglobulin and reducing inflammatory factors.

[0078] In summary, the present invention is based on a specific compound Chinese herbal medicine ratio, and introduces fermented mucus lactobacillus to perform solid-state fermentation to obtain a compound Chinese herbal medicine fermentation preparation, which can further increase the content of active ingredients in Chinese herbal medicines, such as the content of total flavonoids and total polysaccharides, and enhance the biological activity of Chinese herbal medicines. Adding it to the diet of sows can significantly increase the sow's lactation and milk quality, improve the milk protein rate, milk fat rate, lactose rate and non-fat solid content in colostrum, thereby improving the sow's lactation performance and milk nutritional quality. It can further increase the weaning weight of newborn piglets, speed up the production rhythm, and also have a positive impact on the improvement of sows' postpartum inflammation problems and the health and growth and development of piglets.

[0079] The above descriptions are only some preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A compound Chinese herbal medicine fermentation method, characterized in that: The steps include: (1) Preparing a compound Chinese herbal medicine powder: removing impurities from the Chinese herbal medicine raw materials Vaccaria segetalis, Tongcao, Loulus odoratus, and Chuanxiong, washing them, naturally air-drying them, and then crushing and sieving them to obtain the powders of the Chinese herbal medicines. According to the mass ratio of Vaccaria segetalis, Tongcao, Loulus odoratus, and Chuanxiong being 6±0.1:2±0.1:2±0.1:1±0.1, the powders are mixed evenly to obtain the compound Chinese herbal medicine powder; (2) Preparing a Chinese herbal medicine culture medium: mixing the following components in weight percentage: 74.5-75.5% of the compound Chinese herbal medicine powder prepared in (1), 24-25% of soybean powder and 0.4-0.6% of calcium carbonate, uniformly sterilizing by high temperature and high pressure; (3) Inoculation and fermentation of bacterial agents: Inoculate the bacterial agent containing fermented mucus lactobacillus into the Chinese herbal medicine culture medium prepared in (2), adjust the water content, and place it in a breathing zone with a one-way breathing valve. Perform anaerobic fermentation at 35-40°C for 5-9 days to obtain the Chinese herbal medicine fermentation preparation, which is then dried at low temperature and stored for later use.

2. The compound Chinese herbal medicine fermentation method according to claim 1, characterized in that: After the impurities are removed and washed in step (1), each Chinese herbal medicine is naturally air-dried until the water content is less than 11%, and then crushed and passed through a 40-mesh sieve to obtain each Chinese herbal medicine powder.

3. The compound Chinese herbal medicine fermentation method according to claim 1, characterized in that: The sterilization conditions in step (2) are 118-125° C. and 0.15 MPa high-pressure steam sterilization for 20-30 min.

4. The compound Chinese herbal medicine fermentation method according to claim 1, characterized in that: In step (3), the fermented mucus lactobacillus is a cell of the strain with the preservation number of CCTCC NO: M20222085 or its progeny strain or subclone strain.

5. The compound Chinese herbal medicine fermentation method according to claim 1, characterized in that: The form of the bacterial preparation containing fermented mucus lactobacillus in step (3) includes but is not limited to: liquid preparation, solid bacterial preparation, paste bacterial preparation, suspension, emulsion, spray or semi-solid bacterial preparation.

6. The compound Chinese herbal medicine fermentation method according to claim 1, characterized in that: The concentration of Lactobacillus mucilaginosus in the bacterial agent is 1×10 7 ~1×10 9 CFU / mL.

7. The compound Chinese herbal medicine fermentation method according to claim 1, characterized in that: The inoculation amount of the bacterial agent is 3-5% of the weight of the Chinese herbal medicine culture medium.

8. The compound Chinese herbal medicine fermentation method according to claim 1, characterized in that: Adjust the moisture content to 30-50%.

9. A fermentation preparation obtained by the compound Chinese herbal medicine fermentation method according to any one of claims 1 to 8.

10. Use of the fermentation preparation according to claim 9 in the preparation of a feed additive for improving the lactation performance of sows, wherein the addition amount of the fermentation preparation in the basic diet of sows is 1.5-2.5%.

Images