Plant extract and oral probiotic composition capable of rapidly improving breath and preventing decayed teeth and application of plant extract and oral probiotic composition

Abstract

The invention discloses a composition of plant extracts and probiotics and application of the composition to prevention of dental caries, improvement of periodontitis and gingivitis, improvement of ozostomia and breath and reduction of dental plaque. Wherein the composition further comprises a vitamin component. The composition can effectively inhibit and reduce dental plaque, especially cariogenic bacteria and halitosis bacteria, improve oral problems, prevent decayed teeth, improve periodontitis, gingivitis, halitosis and breath, refresh breath, balance oral flora and colonize beneficial bacteria in the oral cavity.

Description

Technical Field

[0001] The present invention belongs to the field of oral health technology, and relates to a composition of plant extracts and probiotics and its use in preventing dental caries, improving periodontitis, gingivitis, improving bad breath, and reducing dental plaque. The composition further includes a vitamin component. The composition can effectively inhibit and reduce dental plaque, especially cariogenic bacteria and bad breath bacteria, improve oral problems, prevent dental caries, improve periodontitis, gingivitis, improve bad breath, freshen breath, balance oral flora, and colonize the oral cavity with beneficial bacteria. Background Art

[0002] The oral environment is a complex environment where multiple bacteria grow together and influence each other. Exogenous factors such as lifestyle and exogenous pathogens can disrupt the symbiotic relationship between the oral microbiome and its host, causing oral diseases such as caries, periodontitis, gingivitis, and halitosis. Many studies have shown that the occurrence of caries and periodontal disease is related to dysbiosis, and the poor growth of some strains may cause local oral diseases such as caries, periodontitis, and may even lead to oral cancer.

[0003] Dental caries is a chronic bacterial oral disease with high incidence, wide distribution and great harm. The World Health Organization lists dental caries as the third most serious chronic non-communicable disease that endangers human health after cancer and cardiovascular disease. In the early stage of dental caries, the bacteria on the surface of the teeth will form a protective film mainly composed of cariogenic bacteria. The most common cariogenic bacteria is Streptococcus mutans.

[0004] The results of the fourth national oral health epidemiological survey show that the caries prevalence rate of primary teeth in 5-year-old children in China is 70.9%, and the caries prevalence rate of permanent teeth in 12-year-old children is 34.5%. In the past 10 years, the caries prevalence rate of 5-year-old and 12-year-old children has increased by 6% and 10% respectively.

[0005] Early caries can easily lead to serious pulp and apical lesions, but the occurrence and development of caries is a reversible process. Timely and effective treatment measures can prevent further deterioration of caries, keep it static or reverse it.

[0006] 90% of bad breath is caused by oral problems. Oral halitosis is mainly caused by Gram-negative anaerobic bacteria in the mouth, such as Fusobacterium nucleatum, Porphyromonas gingivalis and other microbial species, which produce volatile sulfide compounds (VSC) through the decay and digestion of protein-containing nutrients such as food residues retained in the mouth, resulting in the production of odor.

[0007] Probiotics have certain effects on the prevention and treatment of oral diseases, but the research on probiotics is still in the advancement stage, and its effectiveness and safety still need further verification. Summary of the invention

[0008] The object of the present invention is to provide a composition containing plant extracts and probiotics, which can effectively inhibit and reduce dental plaque, especially cariogenic bacteria and halitosis bacteria, improve oral problems, prevent caries, improve periodontitis and gingivitis, improve halitosis, freshen breath, and balance oral flora and colonize beneficial oral bacteria.

[0009] Oral probiotics are a type of beneficial bacteria that can colonize the oral cavity and inhibit the growth and reproduction of pathogenic bacteria. They have strong antibacterial, strong colonization, and low acid production properties, and their metabolites will not cause corrosion and demineralization of teeth.

[0010] The present invention uses oral probiotics specially developed and customized for the oral cavity (preventing tooth decay), which reduce dental plaque by inhibiting oral pathogens, and have better anti-caries and anti-caries effects in children's oral cavity, can quickly improve bad breath and freshen breath. Clinical evidence shows that the probiotics used in the present invention can significantly improve bad breath within 7 days and reduce dental plaque in 6 weeks, thereby achieving the effect of preventing tooth decay.

[0011] The present invention provides a composition of a plant extract and probiotics, wherein the plant extract is a lemon extract, and the total number of viable probiotics is not less than 1×10 9 cfu / serving.

[0012] Lemon extract (Crirus limon (L.) Burm f.) is a natural plant extract with abundant sources, convenient materials, simple usage, significant curative effect and minimal side effects. Lemon extract contains hesperidin, organic acids (such as citric acid), minerals, flavonoids and volatile aromatic oils, and has bactericidal and antiviral effects. Lemon extract can specifically act on Streptococcus mutans and affect the gene expression of Streptococcus mutans. It can also inhibit other cariogenic bacteria (Han Hui et al., Study on the effect of lemon extract on the growth of oral cariogenic bacteria, Stomatological Research, 2012, 28, 2), (Zhang Xiangyu et al. Effect of lemon extract on lactate dehydrogenase and sucrase activity of Streptococcus mutans, Chinese Journal of Stomatology, 2010, 45 (12): 754-758).

[0013] In addition, lemon extract has a fresh smell and is rich in vitamin B and vitamin C, which can prevent bleeding gums and oral ulcers, strengthen gums, promote intestinal excretion, detoxification and beauty.

[0014] Lemon extract has good biosafety. It can achieve a bactericidal effect by destroying bacterial biofilms, and the effect gradually increases with the extension of the action time. Therefore, it can effectively inhibit bacterial proliferation.

[0015] Probiotics are living microorganisms that, when ingested in sufficient quantities, can produce health benefits for the host. They have a positive preventive and therapeutic effect on oral diseases such as caries, periodontitis, halitosis, pharyngitis, and oral mucositis through more subtle mechanisms, including direct inhibition of pathogen growth and regulation of the mucosal immune system. Specifically, they include: (1) direct interaction, causing the binding of oral microorganisms to proteins, inhibiting pathogen adhesion, colonization, and biofilm formation, and inhibiting collagenase activity. (2) competitive exclusion, through competition and intervention with pathogen attachment and participation in substrate metabolism and the production of chemical substances that inhibit oral pathogens. (3) indirect effects, regulating systemic immune function and thus affecting local immunity, ultimately affecting the production of non-immune defense mechanisms; regulating cell proliferation and cytokine-induced apoptosis; inhibiting plaque-induced oral diseases by neutralizing free electrons; and regulating oral mucosal permeability as an antioxidant.

[0016] The present invention creatively combines lemon extract with probiotics, which has the following benefits:

[0017] (1) Rapid onset of effect: Oral probiotics specially developed for oral cavity (prevention of dental caries) are selected, which reduce dental plaque by inhibiting oral pathogens and have better anti-caries and anti-caries effects in children's oral cavity. They can quickly improve bad breath and freshen breath. Clinical evidence shows that the probiotics selected in the present invention can significantly improve bad breath within 7 days and reduce dental plaque in 6 weeks, thereby achieving the effect of preventing dental caries.

[0018] (2) Nutritional supplement: Lemon extract is rich in vitamin C and antioxidants, which help enhance immunity and maintain health. Probiotics help promote intestinal health and digestion and absorption.

[0019] (3) Improved taste: Lemon extract may bring a refreshing sweet and sour taste of lemon to the product, providing a better taste experience.

[0020] (4) Comprehensive effect: Lemon extract can inhibit the growth and reproduction of bacteria, fungi, and viruses, especially has a good inhibitory effect on Streptococcus mutans; probiotics, especially oral probiotics, can regulate the balance of oral microecology, effectively inhibit bad breath pathogens, reduce Streptococcus mutans in saliva, inhibit oral pathogens, quickly freshen breath, and improve oral problems and gum conditions; lemon extract can kill harmful bacteria, and combined with the effects of probiotics, it can inhibit and reduce dental plaque, prevent tooth decay, improve periodontitis and gingivitis, reduce volatile sulfide (VSC), improve bad breath, and at the same time, it can colonize the oral cavity with beneficial bacteria, further balance the oral flora, and improve oral problems.

[0021] The composition containing plant extracts and probiotics of the present invention further comprises vitamins, and further, the vitamin is vitamin D. Vitamin D is an important fat-soluble vitamin that can help absorb calcium and phosphorus, promote the process of tooth remineralization, promote the growth of bones and teeth, provide necessary nutrition for teeth, strengthen teeth and prevent loosening.

[0022] The present invention provides a composition of a plant extract and probiotics, wherein the plant extract is a lemon extract, and the total number of viable probiotics is not less than 1×10 9 cfu / serving.

[0023] In one embodiment, the composition of the present invention, the probiotics include oral probiotics. Further, the oral probiotics include but are not limited to Streptococcus salivarius, Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium longum long subspecies, Bifidobacterium breve, Bifidobacterium catenellae, Streptococcus odontica, Lactobacillus brevis, Lactobacillus salivarius, Lactobacillus casei, Lactobacillus fermentum, Bifidobacterium longum infantis subspecies, Lactobacillus bulgaricus, Lactobacillus gasseri, Lactobacillus crispatus, Lactobacillus reuteri, Bifidobacterium adolescentis, Bifidobacterium bifidum, Streptococcus intermedius, Enterococcus faecalis, Enterococcus faecium, and Saccharomyces boulardii.

[0024] In one embodiment, in the composition of the present invention, the probiotics further include one or more of Lactobacillus plantarum, Lactobacillus paracasei, and Lactobacillus helveticus.

[0025] In a preferred embodiment, the probiotics of the present invention are selected from one or more of Streptococcus salivarius, Lactobacillus plantarum, and Lactobacillus brevis.

[0026] In a preferred embodiment, the total number of viable probiotic bacteria of the present invention is 1×10 9 -1×10 11 cfu / portion. For example, 1×10 9 , 2×10 9 , 2.5×10 9 , 3×10 9 , 3.5×10 9 , 4×10 9 , 4.5×10 9 , 5×10 9 , 5.5×10 9 , 6×10 9 , 6.5×10 9 ,7×10 9 , 7.5×10 9 , 8×10 9 , 8.5×10 9 ,9×10 9 , 9.5×109 , 1×10 10 , 2×10 10 , 2.5×10 10 , 3×10 10 , 3.5×10 10 , 4×10 10 , 4.5×10 10 , 5×10 10 , 6×10 10 ,7×10 10 , 8×10 10 ,9×10 10 , 1×10 11 cfu / portion.

[0027] In a more preferred embodiment, the total number of viable probiotic bacteria of the present invention is 1×10 9 -5×10 10 cfu / portion.

[0028] In a more preferred embodiment, the total number of viable probiotic bacteria of the present invention is 5×10 9 -5×10 10 cfu / serving.

[0029] In a particularly preferred embodiment, the total number of viable probiotic bacteria of the present invention is 1×10 10 -5×10 10 cfu / portion.

[0030] Furthermore, the Streptococcus salivarius in the composition of the present invention is 1×10 5 -1×10 11 cfu / dose, e.g. 1×10 5 , 1×10 6 , 1×10 7 , 1×10 8 , 1×10 9 , 1.5×10 9 , 2×10 9 , 2.5×10 9 , 3×10 9 , 3.5×10 9 , 4×10 9 , 4.5×10 9 , 5×10 9 , 5.5×10 9 , 6×10 9 , 6.5×10 9 ,7×10 9 , 7.5×10 9 , 8×10 9 , 8.5×10 9,9×10 9 , 9.5×10 9 , 1×10 10 , 2×10 10 , 2.5×10 10 , 3×10 10 , 3.5×10 10 , 4×10 10 , 4.5×10 10 , 5×10 10 , 6×10 10 ,7×10 10 , 8×10 10 ,9×10 10 , 1×10 11 cfu / portion.

[0031] Furthermore, the Lactobacillus plantarum in the composition of the present invention is 1×10 5 -1×10 11 cfu / dose, e.g. 1×10 5 , 1×10 6 , 1×10 7 , 1×10 8 , 1×10 9 , 1.5×10 9 , 2×10 9 , 2.5×10 9 , 3×10 9 , 3.5×10 9 , 4×10 9 , 4.5×10 9 , 5×10 9 , 5.5×10 9 , 6×10 9 , 6.5×10 9 ,7×10 9 , 7.5×10 9 , 8×10 9 , 8.5×10 9 ,9×10 9 , 9.5×10 9 , 1×10 10 , 2×10 10 , 2.5×10 10 , 3×10 10 , 3.5×10 10 , 4×10 10 , 4.5×10 10 , 5×10 10 , 6×10 10 ,7×10 10 , 8×10 10 ,9×1010 , 1×10 11 cfu / portion.

[0032] Furthermore, the Lactobacillus brevis in the composition of the present invention is 1×10 5 -1×10 11 cfu / dose, e.g. 1×10 5 , 1×10 6 , 1×10 7 , 1×10 8 , 1×10 9 , 1.5×10 9 , 2×10 9 , 2.5×10 9 , 3×10 9 , 3.5×10 9 , 4×10 9 , 4.5×10 9 , 5×10 9 , 5.5×10 9 , 6×10 9 , 6.5×10 9 ,7×10 9 , 7.5×10 9 , 8×10 9 , 8.5×10 9 ,9×10 9 , 9.5×10 9 , 1×10 10 , 2×10 10 , 2.5×10 10 , 3×10 10 , 3.5×10 10 , 4×10 10 , 4.5×10 10 , 5×10 10 , 6×10 10 ,7×10 10 , 8×10 10 ,9×10 10 , 1×10 11 cfu / portion.

[0033] Furthermore, the probiotic composition of the present invention is 1×10 Streptococcus salivarius 5 -1×10 11 cfu / portion, Lactobacillus plantarum 1×10 5 -1×10 11 cfu / portion, Lactobacillus brevis 1×10 5 -1×10 11 cfu / portion.

[0034] In one embodiment, the composition of the present invention further comprises vitamin D. Furthermore, the vitamin D is 40-150 IU / portion, for example 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150 IU / portion.

[0035] Furthermore, in the composition of the present invention, the vitamin D content is 80-120 IU / portion.

[0036] Furthermore, in the composition of the present invention, the vitamin D is 100 IU / serving.

[0037] In one embodiment, in the composition of the present invention, the lemon extract is 0.1-10 mg / serving.

[0038] Furthermore, the lemon extract is 0.1-5 mg / portion.

[0039] In one embodiment, in the composition of the present invention, the effective components of the lemon extract are one or more of vitamin C and flavonoids.

[0040] All the examples of content are only for illustrative purposes and are not intended to limit the present invention.

[0041] The present invention provides a product comprising the plant extract and probiotics. Further, the product is added with food-acceptable excipients to prepare a preparation form that is easily accepted by the human body, including but not limited to tablets, capsules, powders, oral liquids, sprays, and inhalants. Further, the preparation form of the product is an effervescent tablet or lozenge. Further, the preparation form of the product is a micro-effervescent tablet.

[0042] In one embodiment, the above tablet further comprises one or more of isomalt, embedded citric acid, sodium bicarbonate, lemon flavor, magnesium stearate, steviol glycosides, and peach flavor.

[0043] The present invention also provides the use of any of the plant extracts and probiotics compositions or the products containing the compositions in inhibiting and reducing dental plaque. Further, the dental plaque includes cariogenic bacteria and halitosis bacteria. Further, the dental plaque includes Porphyromonas gingivalis, Streptococcus mutans, Actinobacillus actinomycetemcomitans, and Prevotella intermedia.

[0044] The present invention also provides the use of any of the above-mentioned plant extracts and probiotics compositions or the products containing the above-mentioned compositions in preventing dental caries, improving periodontitis, gingivitis, and improving bad breath.

[0045] Furthermore, the present invention also provides use of any of the compositions of the plant extract and probiotics or the products comprising the composition in preventing dental caries.

[0046] Furthermore, the present invention also provides the use of any of the above-mentioned compositions of plant extracts and probiotics or the above-mentioned products comprising the above-mentioned compositions in improving periodontitis and gingivitis.

[0047] Furthermore, the present invention also provides the use of any of the above-mentioned compositions of plant extracts and probiotics or the products containing the above-mentioned compositions in improving bad breath.

[0048] The present invention provides a composition of a plant extract and probiotics, and further, the composition includes vitamin D. The composition of the present invention uses oral probiotics specially developed and customized for oral cavity (preventing dental caries), which reduces dental plaque by inhibiting oral pathogens, and has better oral caries prevention and anti-caries effects for children. While effectively inhibiting dental plaque, especially cariogenic bacteria, it can also improve oral problems, quickly improve breath, and freshen breath. Clinical evidence shows that the probiotics used in the present invention can significantly improve breath within 7 days and reduce dental plaque in 6 weeks, thereby achieving the effect of preventing dental caries; it also has the functions of maintaining oral health, enhancing immunity and providing comprehensive nutritional support, and can balance oral flora, and colonize the oral cavity with beneficial bacteria.

[0049] Explanation of terms

[0050] As used herein, "probiotics" refers to live microorganisms that, when ingested in adequate amounts, can confer a health benefit on the host. Probiotics primarily colonize the human intestine, but also exist in the oral cavity, vagina, and other mucosal surfaces.

[0051] The "oral probiotics" described in this article are a type of beneficial bacteria that can colonize the oral cavity and inhibit the growth and reproduction of pathogenic bacteria. They have the properties of strong antibacterial, strong colonization, and low acid production, and their metabolites will not cause corrosion and demineralization to teeth.

[0052] The "lemon extract" described herein is a natural plant extract derived from lemon or citrus limon of the Rutaceae family. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] Figure 1 This is a comparison of the color of dental plaque of subject 1;

[0054] Figure 2 This is a comparison chart of dental plaque color of subject 2. DETAILED DESCRIPTION

[0055] In order to facilitate the understanding of those skilled in the art, the present invention is further described below in conjunction with embodiments, and the contents mentioned in the implementation modes are not intended to limit the present invention.

[0056] Example

[0057] Example 1

[0058] A composition containing 2.5×10 mixed probiotics 10 cfu, 1 mg of lemon extract, 100 IU of vitamins, and the mixed probiotics are Streptococcus salivarius, Lactobacillus brevis, and Lactobacillus plantarum.

[0059] Example 2

[0060] A composition containing 2.5×10 mixed probiotics 10 cfu, 0.5 mg lemon extract, 100 IU vitamin, the mixed probiotics are Streptococcus salivarius, Lactobacillus brevis, and Lactobacillus plantarum.

[0061] Example 3

[0062] A composition comprising 2×10 mixed probiotics 10 cfu, 0.5 mg lemon extract, 100 IU vitamin, the mixed probiotics are Streptococcus salivarius, Lactobacillus brevis, and Lactobacillus plantarum.

[0063] Example 4

[0064] A composition comprising 2×10 mixed probiotics 10 cfu, 0.5 mg lemon extract, 100 IU vitamin, the mixed probiotics are Streptococcus salivarius, Lactobacillus brevis, and Lactobacillus plantarum.

[0065] Example 5

[0066] A composition containing 2.5×10 mixed probiotics 10 cfu, 0.1 mg of lemon extract, 100 IU of vitamins, and the mixed probiotics are Streptococcus salivarius, Lactobacillus brevis, and Lactobacillus plantarum.

[0067] Example 6

[0068] A product containing mixed probiotics 2.5×10 10 cfu, 1 mg of lemon extract, 100 IU of vitamins, isomalt, embedded citric acid, sodium bicarbonate, lemon flavor, magnesium stearate, steviol glycosides, peach flavor, and the mixed probiotics are Streptococcus salivarius, Lactobacillus brevis, and Lactobacillus plantarum.

[0069] Example 7 Preparation of formulation

[0070] The raw and auxiliary materials are unpacked, cleaned and disinfected, weighed, and divided into raw and auxiliary materials A and raw and auxiliary materials B. The raw and auxiliary materials A are sieved to obtain premix A, and the raw and auxiliary materials B and the mixed probiotics are sieved to prepare premix B. The premix A and the premix B are mixed, a lubricant is added, and the tableting is checked. After the sieve is dusted, the gold test CCP is performed, and the tablet preparation is obtained by drying.

[0071] Test Case

[0072] Test Example 1: Human test test to evaluate the effect of improving oral problems

[0073] Trial service plan:

[0074] 1. Positive comments include reduced tartar, better plaque detection, improved breath, reduced gum bleeding, and smooth tooth surface; negative comments include no obvious physical sensation and a bitter taste in the mouth after eating.

[0075]

[0076]

[0077] The experimental results show: human trial evaluation.

[0078] 2. Comparison of dental plaque color development results shows that after taking the product for 7 days, dental plaque has been significantly improved. After taking it for more than ten days, dental plaque has almost completely disappeared.

[0079] It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as generally understood by those skilled in the art in the art to which the present invention belongs. It should also be understood that terms such as those defined in general dictionaries should be understood to have meanings consistent with the meanings in the context of the prior art, and will not be interpreted with idealized or overly formal meanings unless specifically defined as herein.

[0080] It should be understood that the detailed description of the technical solutions of the present invention by means of the preferred embodiments is illustrative rather than restrictive. A person skilled in the art may modify the technical solutions described in the embodiments, or replace some of the technical features by equivalents, based on reading the specification of the present invention; and these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition of a plant extract and probiotics, wherein the plant extract is a lemon extract and the total number of viable probiotics is not less than 1×10 9 cfu / portion.

2. The composition according to claim 1, characterized in that The probiotics include oral probiotics.

3. The composition according to claim 2, characterized in that The oral probiotics are one or more of Streptococcus salivarius, Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium longum subsp. longum, Bifidobacterium breve, Bifidobacterium catenella, Streptococcus odontica, Lactobacillus breve, Lactobacillus salivarius, Lactobacillus casei, Lactobacillus fermentum, Bifidobacterium longum subsp. infantis, Lactobacillus bulgaricus, Lactobacillus gasseri, Lactobacillus crispatus, Lactobacillus reuteri, Bifidobacterium adolescentis, Bifidobacterium bifidum, Streptococcus intermedius, Enterococcus faecalis, Enterococcus faecium, and Saccharomyces boulardii.

4. The composition according to claim 2, characterized in that The probiotics also include one or more of Lactobacillus plantarum, Lactobacillus paracasei, and Lactobacillus helveticus.

5. The composition according to claim 4, characterized in that The probiotics are selected from one or more of Streptococcus salivarius, Lactobacillus plantarum and Lactobacillus brevis.

6. The composition according to claim 1, characterized in that The composition further includes vitamin D.

7. The composition according to claim 6, characterized in that The amount of vitamin D added is 40-150 IU / serving.

8. A product characterized by The product comprises the composition according to any one of claims 1 to 7.

9. The product according to claim 8, characterized in that: The product can be prepared into one or more of tablets, capsules, powders, oral liquids, sprays, and inhalants by adding food-acceptable adjuvants.

10. The product according to claim 9, characterized in that The product is prepared into tablets, and further can be prepared into effervescent tablets 、 lozenge.

11. The product according to claim 10, characterized in that: The tablet further comprises one or more of isomalt, embedded citric acid, sodium bicarbonate, lemon flavor, magnesium stearate, steviol glycoside, and peach flavor.

12. The composition according to any one of claims 1 to 8 or the product according to any one of claims 9 to 11, for use in inhibiting and reducing dental plaque, wherein the dental plaque further comprises cariogenic bacteria and halitosis bacteria.

13. The composition according to any one of claims 1 to 8 or the product according to any one of claims 9 to 11, for use in preventing dental caries, improving periodontitis, gingivitis, and improving halitosis.

14. Use of the composition according to any one of claims 1 to 8 or the product according to any one of claims 9 to 11 in preventing dental caries.

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