Micro-nano spray needle manufacturing method based on plant villus and micro-nano spray needle

Abstract

The invention discloses a micro-nano spray needle manufacturing method based on plant villus and a micro-nano spray needle. The manufacturing method of the micro-nano spray needle based on the plant villus comprises the following steps: S1, preparing a plant villus mold by adopting a freezing method; s2, pouring is conducted on the plant villus mold through a pouring method, and a spray needle model which is based on the plant villus and provided with nano channels is obtained; and S3, a micron channel is etched above the spray needle model with the nano channel obtained in the step S2 through a laser method, and the micro-nano spray needle is obtained. According to the micro-nano spray needle manufacturing method based on the plant villus and the micro-nano spray needle, the micro-nano spray needle is manufactured in the mode that the plant villus mold with the nano channel is obtained through the villus on the surface of the plant leaf, and the micro-nano spray needle has the advantages of being simple in preparation process, low in preparation cost and easy to manufacture on a large scale.

Description

Technical Field

[0001] The invention relates to the field of micro-electromechanical technology, and in particular to a method for manufacturing a micro-nano spray needle based on plant hairs and the micro-nano spray needle. Background Art

[0002] With the rapid development of micro-nano technology and the widespread application of micro-nano spray needles, it is urgent to develop low-cost, high-precision micro-nano spray needles.

[0003] In the prior art, micro-nano spray needles can be manufactured using a heating and stretching method, and a micro-nano-scale spray needle is obtained by heating and stretching the material. This method has a simple operation process, but the spray needle size is difficult to accurately control and is prone to damage. In the prior art, micro-nano spray needles can also be manufactured using an angle forming peeling method combined with a casting method. First, a silicon nano concave mold is prepared using an angle forming peeling method, and then a PDMS nano convex mold is obtained by casting. The convex lines are further printed to prepare a micro-nano spray needle convex mold. Finally, an unbonded polymer micro-nano spray needle is obtained by casting, bonded to the PDMS spray needle cover plate, and a steel needle is inserted to obtain the final product. This method has a relatively flexible manufacturing process and can be plug-and-play, but the preparation process has many steps and is relatively complicated, and has high technical requirements for experimental personnel.

[0004] Therefore, how to produce high-precision micro-nano spray needles at low cost and high throughput is one of the key issues that need to be solved urgently. Summary of the invention

[0005] The present invention overcomes the shortcomings of the prior art and provides a method for manufacturing a micro-nano spray needle based on plant hairs and a micro-nano spray needle. The micro-nano spray needle is manufactured by utilizing the hairs on the surface of plant leaves to obtain a plant hair mold with nanochannels. The method has the advantages of simple preparation process, low preparation cost, and easy mass production.

[0006] To achieve the above object, the technical solution adopted by the present invention is: a method for manufacturing a micro-nano spray needle based on plant hair, comprising the following steps: Step S1, preparing a plant fluff mold by a freezing method; Step S2, using a casting method to cast the plant hair mold to obtain a spray needle model with nanochannels based on the plant hair; Step S3, using a laser method to etch a micron channel on the nozzle model with a nanochannel obtained in step S2 to obtain a micro-nano nozzle.

[0007] In a preferred embodiment of the present invention, the plant fluff mold is prepared by a freezing method in step S1, comprising the following steps: Step S1.1, obtaining a plant body with plant hairs, wherein the cross-sectional diameter of the plant hairs is nanometer-scale; Step S1.2, pre-treating the plant body with plant hairs; Step S1.3, laying the plant body with plant hairs processed in step S1.2 flat and fixing it on a glass substrate; Step S1.4, freezing the plant body with plant hairs fixed on the glass substrate by freezing method, and spraying trimethylchlorosilane on the plant body with plant hairs during freezing to obtain a plant hair mold.

[0008] In a preferred embodiment of the present invention, the plant body with plant hairs is pretreated in step S1.2, comprising the following steps: Step S1.2.1, placing the plant body with plant hairs in deionized water for ultrasonic cleaning; Step S1.2.2, drying the plant body with plant hairs after ultrasonic cleaning; Step S1.2.3, trimming the plant body with plant hair after drying; The freezing method comprises: Step S1.4.1, spraying deionized water mist on the plant body with plant hairs fixed on the glass substrate to obtain a moist plant body with plant hairs; Step S1.4.2, placing the moist plant body with plant hairs in a freezing environment and freezing it.

[0009] In a preferred embodiment of the present invention, in step S2, the pouring method comprises the following steps: Step S2.1, the PDMS mixed solution is uniformly flowed from the periphery of the plant fluff mold to the center and covers the plant fluff mold to obtain a PDMS plant fluff mold; Step S2.2, place the PDMS plant hair mold on a level platform for curing.

[0010] Step S2.3, separating the PDMS solidified body in the PDMS plant hair mold from the plant hair mold to obtain a needle model with nanochannels.

[0011] In a preferred embodiment of the present invention, in step S3, a laser method is used to etch a micron channel on the nozzle model with a nanochannel obtained in step S2 to obtain a micro-nano nozzle, comprising the following steps: A clean nozzle model with nanochannels is placed on a laser three-dimensional platform, and a micron channel with a diameter of 50 to 200 μm connected to the nanochannel in the nozzle model is printed on the clean nozzle model with nanochannels by laser etching. Each micron channel is connected to several nanochannels to obtain a PDMS nozzle model with microchannels and nanochannels. In a preferred embodiment of the present invention, the micro-nano spray needle obtained in step S3 is a single-channel micro-nano spray needle, comprising the following steps: A clean nozzle model with nanochannels is placed on a laser three-dimensional platform, and a micron channel with a diameter of 50-200 μm connected to the nanochannel in the nozzle model is printed one-to-one on the clean nozzle model with nanochannels by laser etching. Each micron channel is connected to a nanochannel to obtain a single-pass nozzle model with microchannels and nanochannels. The printing laser is a carbon dioxide laser with a laser power of 10-20 W and a speed of 20-30 mm / s. The single-pass nozzle model with microchannels and nanochannels is grouped and segmented using a coating tool, and a steel needle treated with oxygen plasma hydrophilicity is inserted into the microchannel for interference fit to obtain a single-channel micro-nano nozzle.

[0012] And / or, the micro-nano spray needle obtained in step S3 is a multi-channel micro-nano spray needle, comprising the following steps: A clean nozzle model with nanochannels is placed on a laser three-dimensional platform, and micron channels with a diameter of 50-200 μm connected to the nanochannels in the nozzle model are printed one-to-one on the clean nozzle model with nanochannels by laser etching. Each micron channel is connected to a nanochannel to obtain a multi-channel nozzle model with micron and nanochannels. The printing laser is a carbon dioxide laser with a laser power of 10-20 W and a speed of 20-30 mm / s. The multi-channel nozzle model with micron and nanochannels is cut into parts using a coated tool, each part has 2-4 connected micron and nanochannels, and a steel needle treated with oxygen plasma hydrophilicity is inserted into the micron channel for interference fit to obtain a multi-channel micro-nano nozzle.

[0013] And / or, the micro-nano spray needles obtained in step S3 are array micro-nano spray needles, comprising the following steps: A clean needle spray model with nanochannels was placed on a laser three-dimensional platform, and micron channels with a diameter of 50-200 μm connected to the nanochannels in the needle spray model were printed one-to-one on the clean needle spray model with nanochannels by laser etching. Each micron channel was connected to 2-4 nanochannels, and an array needle spray model with micron and nanochannels was obtained. The printing laser was a carbon dioxide laser with a laser power of 20-30 W and a speed of 20-30 mm / s. The array needle spray model with micron and nanochannels was cut into parts using a coated tool, and each part had a micron channel connected to 2-4 nanochannels, and a steel needle treated with oxygen plasma hydrophilicity was inserted into the micron channel for interference fit to obtain an array micro-nano needle spray.

[0014] In a preferred embodiment of the present invention, in step S1.2.1, the plant body with plant hairs is placed in deionized water for ultrasonic cleaning; the working time is 15-25 minutes, the power is 20-60W, and the temperature is 20-30°C; And / or, in step S1.2.2, the plant body with plant hairs after ultrasonic cleaning is placed in the sun for 6 to 8 hours at room temperature to remove moisture on the surface of the plant hairs of the plant body and achieve drying; And / or, in step S1.2.3, the plant body with plant hairs after drying is cut into leaf strips with a width of 5 to 10 mm using a coated cutter, and leaf strips that meet the preparation requirements are selected to trim the plant body with plant hairs after drying; And / or, in step S1.4.2, the moist plant body with plant hairs is placed in a freezing environment at -30 to -15°C for 3 to 5 hours.

[0015] In a preferred embodiment of the present invention, the method for preparing the PDMS mixed solution in step S2.1 comprises: mixing a PDMS base material and a curing agent in a volume ratio of 5:1 to 10:1, stirring for 5 to 10 minutes to obtain a PDMS mixed solution, and placing the PDMS mixed solution in a vacuum box for 20 to 30 minutes to degas and remove bubbles inside the PDMS mixed solution; and / or, step S2.2, placing the PDMS plant hair mold on a leveled platform and curing it at room temperature for 18 to 32 hours; And / or, in step S2.3, after the PDMS solidified body in the PDMS plant hair mold is separated from the plant hair mold, the method further includes: placing the nanochannel side of the PDMS needle model with nanochannels downward in deionized water for ultrasonic cleaning to remove the residual plant hair in the nanochannels; and placing it on a hot plate for heating to obtain a clean needle model with nanochannels.

[0016] In a preferred embodiment of the present invention, the plant body with plant fluff used to prepare the plant fluff mold includes one of creeper leaves, lotus and lotus; And / or, when ultrasonically cleaning a plant body with plant hairs, placing the leaves face down can effectively protect the plant hairs and facilitate the discharge of dirt between the hairs; and / or, when selecting leaf strips of a plant body with plant hairs, selecting leaf strips in which hairs on the leaf strips are arranged longitudinally in a single row; and / or, in step S2.3, the needle model with the nanochannel is placed with the concave surface facing downward, and the power of ultrasonic cleaning is 100-300W to remove the residual plant hair; And / or, in step S2.3, the heating temperature of the heating on the hot plate is 60-90° C., and the heating time is 20-30 min.

[0017] In a preferred embodiment of the present invention, a plant hair-based micro-nano spray needle is prepared by a plant hair-based micro-nano spray needle manufacturing method.

[0018] The present invention solves the defects existing in the technical background, and the beneficial technical effects of the present invention are: The present invention discloses a method for manufacturing a micro-nano spray needle based on plant hairs and a micro-nano spray needle. The micro-nano spray needle is manufactured by using the hairs on the surface of plant leaves to obtain a plant hair mold with nanochannels. The method has the advantages of simple preparation process, low preparation cost, and easy mass production.

[0019] 1. The present invention is based on the freezing casting method of the villi of the ivy leaves, and adopts the strategy of "surrounding the center" to manufacture the nano-needle channel.

[0020] 2. The present invention adopts laser etching to manufacture micron channels, and obtains single-channel micro-nano spray needles, multi-channel micro-nano spray needles and array micro-nano spray needles through different cutting methods. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0022] Figure 1 This is a flow chart of pre-treatment of Parthenocissus tricuspidata leaves in a preferred embodiment of the present invention; Figure 2 This is a flow chart of preparing nanochannels of a spray needle by freeze casting in a preferred embodiment of the present invention; Figure 3 It is a flow chart of preparing a micron channel of a spray needle by laser etching in a preferred embodiment of the present invention; Figure 4 Schematic diagram of the structure of a single-channel micro-nano spray needle in a preferred embodiment of the present invention; Figure 5 Schematic diagram of the structure of a multi-channel micro-nano spray needle in a preferred embodiment of the present invention; Figure 6 Schematic diagram of the structure of the array micro-nano spray needle in the preferred embodiment of the present invention; Figure 7 This is a high-power tool microscope image of plant hairs on the surface of Parthenocissus tricuspidata leaves in a preferred embodiment of the present invention; Figure 8 is a SEM image of a nanochannel cross section of a single-channel micro-nano spray needle in a PDMS micro-nano spray needle in a preferred embodiment of the present invention; Fig. 9is a SEM image of a nanochannel cross section of a multi-channel micro-nano spray needle in a PDMS micro-nano spray needle in a preferred embodiment of the present invention; In the figure: 1. Ivy leaves; 2. Ultrasonic cleaning machine; 3. Sprayer; 4. Leaf strips; 5. Freezing environment; 6. PDMS solid body; 7. Spray needle model; 8. Hot plate; 10. Nanochannel; 11. Microchannel; 12. Steel needle. DETAILED DESCRIPTION

[0023] The present invention will now be further described in detail with reference to the accompanying drawings and embodiments. These drawings are simplified schematic diagrams that only illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0024] It should be noted that if there are directional indications (such as up, down, bottom, top, etc.) involved in the embodiments of the present invention, the directional indication is only used to explain the relative position relationship, movement, etc. between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly. The terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. Unless otherwise clearly specified and defined, the terms "set", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected, or indirectly connected through an intermediate medium, or it can be the internal connection of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0025] like Figure 1-Figure 6 As shown, a method for manufacturing a micro-nano spray needle based on plant hairs comprises the following steps: Step S1, preparing a plant fluff mold by a freezing method.

[0026] The plant fluff mold is prepared by a freezing method, comprising the following steps: Step S1.1, obtaining a plant body with plant hairs, wherein the cross-sectional diameter of the plant hairs is nanometer-level. In this embodiment, the plant body with plant hairs used to prepare the plant hair mold is a creeper leaf 1. However, this is not limited to this. In other embodiments, other plant bodies with plant hairs can be selected according to actual use requirements, and the diameter of the plant hairs is nanometer-level.

[0027] Step S1.2, pre-treating the plant body with plant hairs; In step S1.2, the plant body with plant hair is pretreated, including the following steps: Step S1.2.1, placing the plant body with plant hair in deionized water of ultrasonic cleaning machine 2 for ultrasonic cleaning. Specifically, in step S1.2.1, placing the plant body with plant hair in deionized water for ultrasonic cleaning; the working time is 15-25 minutes, the power is 20-60W, and the temperature is 20-30°C. More specifically, when ultrasonic cleaning the plant body with plant hair, the leaves are placed face down, which can effectively protect the plant hair and facilitate the discharge of dirt between the hairs.

[0028] Step S1.2.2, drying the plant body with plant hair after ultrasonic cleaning. Specifically, in step S1.2.2, the plant body with plant hair after ultrasonic cleaning is placed in the sun at room temperature for 6 to 8 hours to remove moisture on the surface of the plant hair of the plant body and achieve drying.

[0029] Step S1.2.3, trimming the plant body with plant hair after drying. Specifically, in step S1.2.3, a coated tool is used to cut the plant body with plant hair after drying into leaf strips 4 with a width of 5 to 10 mm, and leaf strips 4 that meet the preparation requirements are selected to trim the plant body with plant hair after drying. More specifically, when selecting the leaf strips 4 of the plant body with plant hair, the leaf strips 4 with hairs on the leaf strips 4 arranged in a single row longitudinally are selected.

[0030] Step S1.3, the plant body with plant hairs processed in step S1.2 is laid flat and fixed on a glass substrate. However, this is not limited to this, and in other embodiments, the glass substrate can be replaced by a substrate of other materials as long as it is suitable for the preparation requirements of this application.

[0031] Step S1.4, freezing the plant body with plant hair fixed on the glass substrate by freezing method, and spraying trimethylchlorosilane on the plant body with plant hair during freezing to obtain a hydrophobic plant hair mold with a certain hardness. The hydrophobic range is 70-110°.

[0032] The freezing method comprises: Step S1.4.1, spraying deionized water mist on the plant body with plant hairs fixed on the glass substrate by using the sprayer 3 to obtain a moist plant body with plant hairs; Step S1.4.2, placing the moist plant body with plant hair in a freezing environment 5 for freezing. Specifically, in step S1.4.2, placing the moist plant body with plant hair in a freezing environment 5 at -30 to -15°C for freezing for 3 to 5 hours.

[0033] Step S2, using a casting method to cast the plant hair mold to obtain a plant hair-based injection needle model 7 with nanochannels 10.

[0034] In step S2, the casting method comprises the following steps: Step S2.1, the PDMS mixed solution is uniformly flowed from the periphery of the plant fluff mold to the center and covers the plant fluff mold to obtain a PDMS plant fluff mold. Specifically, the preparation method of the PDMS mixed solution in step S2.1 includes: mixing the PDMS base material and the curing agent in a volume ratio of 5:1 to 10:1, stirring for 5 to 10 minutes to obtain a PDMS mixed solution, and placing the PDMS mixed solution in a vacuum box for 20 to 30 minutes to degas and remove bubbles inside the PDMS mixed solution.

[0035] Step S2.2, placing the PDMS plant hair mold on a leveled horizontal platform for curing. Specifically, step S2.2, placing the PDMS plant hair mold on a leveled horizontal platform for curing at room temperature for 18 to 32 hours.

[0036] Step S2.3, separate the PDMS solidified body 6 solidified in the PDMS plant fluff mold from the plant fluff mold to obtain the spray needle model 7 with the nanochannel 10. Specifically, in step S2.3, after separating the PDMS solidified body 6 solidified in the PDMS plant fluff mold from the plant fluff mold, it also includes: placing the nanochannel 10 of the PDMS spray needle model with the nanochannel 10 face down in the deionized water of the ultrasonic cleaning machine 2 for ultrasonic cleaning to remove the residual plant fluff in the nanochannel 10; and placing it on the hot plate 8 for heating to obtain a clean spray needle model 7 with the nanochannel 10. More specifically, in step S2.3, the spray needle model 7 with the nanochannel 10 is placed with the concave surface facing down, the power of ultrasonic cleaning is 100~300W, and the residual plant fluff is removed; the heating temperature for heating on the hot plate 8 is 60~90℃, and the heating time is 20~30min.

[0037] Step S3, using a laser method to etch a micron channel 11 on the nozzle model 7 with the nanochannel 10 obtained in step S2 to obtain a micro-nano nozzle.

[0038] In step S3, a micron channel 11 is etched on the nozzle model 7 with the nanochannel 10 obtained in step S2 by a laser method to obtain a micro-nano nozzle, which includes the following steps: A clean needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 50 to 200 μm connected to the nanochannel 10 in the needle model is printed one-to-one on the clean needle model 7 with a nanochannel 10 by laser etching. Each micron channel 11 is connected to several nanochannels 10 to obtain a needle model 7 with micron channels 11 and nanochannels 10.

[0039] In one embodiment, the micro-nano spray needle obtained in step S3 is a single-channel micro-nano spray needle, comprising the following steps: A clean needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 50-200 μm connected to the nanochannel 10 in the needle model is printed one-to-one on the clean needle model 7 with a nanochannel 10 by laser etching. Each micron channel 11 is connected to a nanochannel 10, so that a single-pass needle model with a micron channel 11 and a nanochannel 10 is obtained; wherein, the printing laser is a carbon dioxide laser, the laser power is 10-20 W, and the speed is 20-30 mm / s; with one connected micron channel 11 and nanochannel 10 as a group, the single-pass needle model with the micron channel 11 and the nanochannel 10 is grouped and divided using a coating tool, and a steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit, so as to obtain a single-channel micro-nano needle.

[0040] In another embodiment, the micro-nano spray needle obtained in step S3 is a multi-channel micro-nano spray needle, comprising the following steps: A clean needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 50-200 μm connected to the nanochannel 10 in the needle model 7 is printed one-to-one on the clean needle model 7 with the nanochannel 10 by laser etching, and each micron channel 11 is connected to a nanochannel 10, so as to obtain a multi-channel needle model with micron channels 11 and nanochannels 10; wherein the printing laser is a carbon dioxide laser, the laser power is 10-20 W, and the speed is 20-30 mm / s; the multi-channel needle model with the micron channel 11 and the nanochannel 10 is cut into parts by using a coated tool, each part has 2-4 connected micron channels 11 and nanochannels 10, and a steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit, so as to obtain a multi-channel micro-nano needle.

[0041] In another embodiment, the micro-nano spray needles obtained in step S3 are array micro-nano spray needles, comprising the following steps: A clean needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 50-200 μm connected to the nanochannel 10 in the needle model 7 is printed one-to-one on the clean needle model 7 with a nanochannel 10 by laser etching. Each micron channel 11 is connected to 2-4 nanochannels 10, and an array needle model with micron channels 11 and nanochannels 10 is obtained, wherein the printing laser is a carbon dioxide laser with a laser power of 20-30 W and a speed of 20-30 mm / s; the array needle model with micron channels 11 and nanochannels 10 is cut into parts using a coated tool, and each part has one micron channel 11 connected to 2-4 nanochannels 10, and a steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit, to obtain an array micro-nano needle. Embodiment 1

[0042] like Figure 1-Figure 6 As shown, in order to achieve the above purpose, the technical solution adopted by the present invention is: a method for manufacturing a micro-nano spray needle based on plant hair, comprising the following steps: Step S1, preparing a plant fluff mold by a freezing method.

[0043] The plant fluff mold is prepared by a freezing method, comprising the following steps: Step S1.1, obtaining a plant body with plant hairs, wherein the diameter of the plant hairs is nanometer-level. In this embodiment, the plant body with plant hairs used to prepare the plant hair mold is a creeper leaf 1. However, this is not limited to this. In other embodiments, other plant bodies with plant hairs can be selected according to actual use requirements, and the diameter of the plant hairs is nanometer-level.

[0044] Step S1.2, pre-treating the plant body with plant hairs; In step S1.2, the plant body with plant hair is pretreated, including the following steps: Step S1.2.1, placing the plant body with plant hairs in the deionized water of ultrasonic cleaning machine 2 for ultrasonic cleaning (such as Figure 1 1a). Specifically, in step S1.2.1, the plant body with plant hairs is placed in deionized water for ultrasonic cleaning; the working time is 15 minutes, the power is 20W, and the temperature is 20°C. More specifically, when ultrasonic cleaning the plant body with plant hairs, the leaves are placed face down, which can effectively protect the plant hairs and facilitate the discharge of dirt between the hairs.

[0045] Step S1.2.2, drying the plant body with plant hair after ultrasonic cleaning. Specifically, in step S1.2.2, the plant body with plant hair after ultrasonic cleaning is placed in the sun at room temperature for 6 hours to remove moisture on the surface of the plant hair of the plant body and achieve drying.

[0046] Step S1.2.3, trimming the plant body with plant hair after drying. Specifically, in step S1.2.3, the coated cutter is used to cut the plant body with plant hair after drying into leaf strips 4 with a width of 5 mm (such as Figure 1 1b), select leaf strips 4 that meet the preparation requirements; and trim the plant body with plant hairs after drying. More specifically, when selecting leaf strips 4 of the plant body with plant hairs, select leaf strips 4 with hairs on the leaf strips 4 arranged in a single row longitudinally.

[0047] Step S1.3, the plant body with plant hairs processed in step S1.2 is flatly fixed on a glass substrate.

[0048] Step S1.4, freezing the plant body with plant hair fixed on the glass substrate by freezing method, and spraying trimethylchlorosilane on the plant body with plant hair during freezing to obtain a hydrophobic plant hair mold with a certain hardness.

[0049] The freezing method comprises: Step S1.4.1, spraying deionized water mist on the plant body with plant hair fixed on the glass substrate through the sprayer 3 to obtain a wet plant body with plant hair (such as Figure 1 (as shown in 1c); Step S1.4.2, placing the wet plant body with plant hair in a freezing environment 5 for freezing. Specifically, in step S1.4.2, placing the wet plant body with plant hair in a freezing environment 5 at -30°C for freezing for 3 hours (e.g. Figure 1 1d).

[0050] Step S2, using a casting method to cast the plant hair mold to obtain a spray needle model 7 with nanochannels 10 based on plant hair (such as Figure 2 as shown).

[0051] In step S2, the casting method comprises the following steps: Step S2.1, the PDMS mixed solution is uniformly flowed from the periphery of the plant hair mold to the center and covers the plant hair mold to obtain a PDMS plant hair mold. Specifically, the preparation method of the PDMS mixed solution in step S2.1 includes: mixing the PDMS base material and the curing agent in a volume ratio of 5:1, stirring for 5 minutes to obtain a PDMS mixed solution, and placing the PDMS mixed solution in a vacuum box for 20 minutes to degas, and removing bubbles (such as Figure 2 2a).

[0052] Step S2.2, placing the PDMS plant hair mold on a leveled horizontal platform for curing. Specifically, step S2.2, placing the PDMS plant hair mold on a leveled horizontal platform for curing at room temperature for 18 hours.

[0053] Step S2.3, separating the PDMS solidified body 6 solidified in the PDMS plant hair mold from the plant hair mold to obtain the needle model 7 having the nanochannel 10. Specifically, in step S2.3, separating the PDMS solidified body 6 solidified in the PDMS plant hair mold from the plant hair mold (such as Figure 2 2b), further comprising: placing the nanochannel 10 of the PDMS needle model having the nanochannel 10 facing downward in the deionized water of an ultrasonic cleaning machine 2 for ultrasonic cleaning (as shown in FIG. Figure 2 2c), remove the remaining plant hair in the nanochannel 10; and place it on a hot plate 8 for heating to obtain a clean needle model 7 with a nanochannel 10 (as shown in Figure 2 More specifically, in step S2.3, the needle model 7 with the nanochannel 10 is placed with the concave surface facing downward, and the power of ultrasonic cleaning is 100 W to remove the residual plant hair; the heating temperature of the needle model 7 placed on the hot plate 8 is 60° C., and the heating time is 20 min.

[0054] Step S3, using a laser method to etch a micron channel 11 on the nozzle model 7 with the nanochannel 10 obtained in step S2 to obtain a micro-nano nozzle.

[0055] In step S3, a micron channel 11 is etched on the nozzle model 7 with the nanochannel 10 obtained in step S2 by a laser method to obtain a micro-nano nozzle, which includes the following steps: like Figure 3 As shown, a clean needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 50 μm connected to the nanochannel 10 in the needle model 7 is printed one-to-one on the clean needle model 7 with a nanochannel 10 by laser etching. Each micron channel 11 is connected to several nanochannels 10, and a needle model 7 with micron channels 11 and nanochannels 10 is obtained.

[0056] In one embodiment, when a single-channel micro-nano spray needle is to be prepared, the micro-nano spray needle obtained in step S3 is a single-channel micro-nano spray needle, comprising the following steps: Place the clean needle model 7 with nanochannel 10 on a laser three-dimensional platform (such as Figure 3 As shown in 3a1, a micron channel 11 with a diameter of 50 μm (as shown in FIG. 3a1 ) is printed one-to-one on the clean needle model 7 having the nanochannel 10 and connected to the nanochannel 10 in the needle model by laser etching. Figure 3 As shown in 3b1 in the figure, each micron channel 11 is connected to a corresponding nanochannel 10, and a single-pass needle model having a micron channel 11 and a nanochannel 10 is obtained (as shown in Figure 3 3c1 in the figure); wherein the printing laser is a carbon dioxide laser, the laser power is 10W, and the speed is 20mm / s; a single-pass needle model having a connected micro-channel 11 and a nano-channel 10 is grouped and segmented using a coating tool (such as Figure 3 As shown in 3d1, the steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit to obtain a single-channel micro-nano spray needle (as shown in 3d1). Figure 4 as shown).

[0057] In another embodiment, when a multi-channel micro-nano spray needle is to be prepared, the micro-nano spray needle obtained in step S3 is a multi-channel micro-nano spray needle, comprising the following steps: Place the clean needle model 7 with nanochannel 10 on a laser three-dimensional platform (such as Figure 3 As shown in 3a1 , a micron channel 11 with a diameter of 50 μm connected to the nanochannel 10 in the spray needle model is printed one-to-one on the clean spray needle model 7 with the nanochannel 10 by laser etching, and each micron channel 11 is connected to a nanochannel 10 (as shown in Figure 3 3b1), a multi-channel needle model with a micron channel 11 and a nanochannel 10 is obtained (as shown in Figure 3 3c2 in the figure); wherein the printing laser is a carbon dioxide laser, the laser power is 10W, and the speed is 20mm / s; the multi-channel needle model having the micron channel 11 and the nano-channel 10 is cut into parts using a coated tool, and each part has two connected micron channels 11 and nano-channels 10 (as shown in Figure 3 As shown in 3d2, the steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit to obtain a multi-channel micro-nano spray needle (such as Figure 5 as shown).

[0058] In another embodiment, when an array micro-nano spray needle is to be prepared, the micro-nano spray needle obtained in step S3 is an array micro-nano spray needle, comprising the following steps: Place the clean needle model 7 with nanochannel 10 on a laser three-dimensional platform (such as Figure 3 As shown in 3a2, a micron channel 11 with a diameter of 50 μm (as shown in FIG. 3a2 ) is printed one-to-one on the clean needle model 7 having the nanochannel 10 and connected to the nanochannel 10 in the needle model by laser etching. Figure 3 As shown in 3b2, each micron channel 11 is connected to two nano channels 10, and an array needle model having micro channels 11 and nano channels 10 is obtained (as shown in Figure 3 3c3), wherein the printing laser is a carbon dioxide laser, the laser power is 20W, and the speed is 20mm / s; the array needle model with micron channels 11 and nano channels 10 is cut into parts using a coated tool, and each part has one micron channel 11 corresponding to two nano channels 10 (as shown in FIG. Figure 3 As shown in 3d3, the steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit to obtain an array of micro-nano spray needles (such as Figure 6 as shown). Embodiment 2

[0059] like Figure 1-Figure 6 As shown, in order to achieve the above purpose, the technical solution adopted by the present invention is: a method for manufacturing a micro-nano spray needle based on plant hair, comprising the following steps: Step S1, preparing a plant fluff mold by a freezing method.

[0060] The plant fluff mold is prepared by a freezing method, comprising the following steps: Step S1.1, obtaining a plant body with plant hairs, wherein the diameter of the plant hairs is nanometer-level. In this embodiment, the plant body with plant hairs used to prepare the plant hair mold is a creeper leaf 1. However, this is not limited to this. In other embodiments, other plant bodies with plant hairs can be selected according to actual use requirements, and the diameter of the plant hairs is nanometer-level.

[0061] Step S1.2, pre-treating the plant body with plant hairs; In step S1.2, the plant body with plant hair is pretreated, including the following steps: Step S1.2.1, placing the plant body with plant hair in deionized water of ultrasonic cleaning machine 2 for ultrasonic cleaning. Specifically, in step S1.2.1, placing the plant body with plant hair in deionized water for ultrasonic cleaning; working time 25min, power 60W, temperature 30°C. More specifically, when ultrasonic cleaning the plant body with plant hair, placing the leaves face down can effectively protect the plant hair and facilitate the discharge of dirt between the hairs.

[0062] Step S1.2.2, drying the plant body with plant hair after ultrasonic cleaning. Specifically, in step S1.2.2, the plant body with plant hair after ultrasonic cleaning is placed in the sun at room temperature for 6 to 8 hours to remove moisture on the surface of the plant hair of the plant body and achieve drying.

[0063] Step S1.2.3, trimming the plant body with plant hair after drying. Specifically, in step S1.2.3, a coated tool is used to cut the plant body with plant hair after drying into leaf strips 4 with a width of 10 mm, and leaf strips 4 that meet the preparation requirements are selected to achieve trimming of the plant body with plant hair after drying. More specifically, when selecting the leaf strips 4 of the plant body with plant hair, the leaf strips 4 with hairs on the leaf strips 4 arranged in a single row longitudinally are selected.

[0064] Step S1.3, the plant body with plant hairs processed in step S1.2 is flatly fixed on a glass substrate.

[0065] Step S1.4, freezing the plant body with plant hair fixed on the glass substrate by freezing method, and spraying trimethylchlorosilane on the plant body with plant hair during freezing to obtain a hydrophobic plant hair mold with a certain hardness.

[0066] The freezing method comprises: Step S1.4.1, spraying deionized water mist on the plant body with plant hairs fixed on the glass substrate by using the sprayer 3 to obtain a moist plant body with plant hairs; Step S1.4.2, placing the moist plant body with plant hair in a freezing environment 5 for freezing. Specifically, in step S1.4.2, placing the moist plant body with plant hair in a freezing environment 5 at -15°C for freezing for 5 hours.

[0067] Step S2, using a casting method to cast the plant hair mold to obtain a plant hair-based injection needle model 7 with nanochannels 10.

[0068] In step S2, the casting method comprises the following steps: Step S2.1, the PDMS mixed solution is uniformly flowed from the periphery of the plant fluff mold to the center and covers the plant fluff mold to obtain a PDMS plant fluff mold. Specifically, the preparation method of the PDMS mixed solution in step S2.1 includes: mixing the PDMS base material and the curing agent in a volume ratio of 10:1, stirring for 10 minutes to obtain a PDMS mixed solution, and placing the PDMS mixed solution in a vacuum box for 30 minutes to degas and remove bubbles inside the PDMS mixed solution.

[0069] Step S2.2, placing the PDMS plant hair mold on a leveled horizontal platform for curing. Specifically, step S2.2, placing the PDMS plant hair mold on a leveled horizontal platform for curing at room temperature for 32 hours.

[0070] Step S2.3, separate the PDMS solidified body 6 solidified in the PDMS plant fluff mold from the plant fluff mold to obtain the spray needle model 7 with the nanochannel 10. Specifically, in step S2.3, after separating the PDMS solidified body 6 solidified in the PDMS plant fluff mold from the plant fluff mold, it also includes: placing the nanochannel 10 of the PDMS spray needle model with the nanochannel 10 face down in deionized water for ultrasonic cleaning to remove the residual plant fluff in the nanochannel 10; and placing it on a hot plate 8 for heating to obtain a clean spray needle model 7 with the nanochannel 10. More specifically, in step S2.3, the concave surface of the spray needle model 7 with the nanochannel 10 is placed downward, the power of ultrasonic cleaning is 300W, and the residual plant fluff is removed; the heating temperature for heating on the hot plate 8 is 90°C, and the heating time is 30min.

[0071] Step S3, using a laser method to etch a micron channel 11 on the nozzle model 7 with the nanochannel 10 obtained in step S2 to obtain a micro-nano nozzle.

[0072] In step S3, a micron channel 11 is etched on the nozzle model 7 with the nanochannel 10 obtained in step S2 by a laser method to obtain a micro-nano nozzle, which includes the following steps: A clean needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 200 μm connected to the nanochannel 10 in the needle model is printed one-to-one on the clean needle model 7 with a nanochannel 10 by laser etching. Each micron channel 11 is connected to several nanochannels 10 to obtain a PDMS needle model with micron channels 11 and nanochannels 10.

[0073] In one embodiment, when a single-channel micro-nano spray needle is to be prepared, the micro-nano spray needle obtained in step S3 is a single-channel micro-nano spray needle, comprising the following steps: A clean needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 200 μm connected to the nanochannel 10 in the needle model is printed one-to-one on the clean needle model 7 with a nanochannel 10 by laser etching. Each micron channel 11 is connected to a nanochannel 10, so that a single-pass needle model with a micron channel 11 and a nanochannel 10 is obtained; wherein the printing laser is a carbon dioxide laser with a laser power of 20 W and a speed of 30 mm / s; with one connected micron channel 11 and nanochannel 10 as a group, the single-pass needle model with the micron channel 11 and the nanochannel 10 is grouped and divided using a coating tool, and a steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit, so as to obtain a single-channel micro-nano needle.

[0074] In another embodiment, when a multi-channel micro-nano spray needle is to be prepared, the micro-nano spray needle obtained in step S3 is a multi-channel micro-nano spray needle, comprising the following steps: A clean needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 200 μm connected to the nanochannel 10 in the needle model is printed one-to-one on the clean needle model 7 with a nanochannel 10 by laser etching, and each micron channel 11 is connected to a nanochannel 10, so as to obtain a multi-channel needle model with micron channels 11 and nanochannels 10; wherein the printing laser is a carbon dioxide laser, the laser power is 20 W, and the speed is 30 mm / s; the multi-channel needle model with the micron channel 11 and the nanochannel 10 is cut into parts by using a coated tool, each part has 4 connected micron channels 11 and nanochannels 10, and a steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit, so as to obtain a multi-channel micro-nano needle.

[0075] In another embodiment, when an array micro-nano spray needle is to be prepared, the micro-nano spray needle obtained in step S3 is an array micro-nano spray needle, comprising the following steps: A clean needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 200 μm connected to the nanochannel 10 in the needle model is printed one-to-one on the clean needle model 7 with a nanochannel 10 by laser etching. Each micron channel 11 is connected to 2 to 4 nanochannels 10, and an array needle model with micron channels 11 and nanochannels 10 is obtained, wherein the printing laser is a carbon dioxide laser with a laser power of 30 W and a speed of 30 mm / s; the array needle model with micron channels 11 and nanochannels 10 is cut into parts using a coated tool, and each part has one micron channel 11 connected to 2 to 4 nanochannels 10, and a steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit, to obtain an array micro-nano needle. Embodiment 3

[0076] like Figure 1-Figure 9 As shown, in order to achieve the above purpose, the technical solution adopted by the present invention is: a method for manufacturing a micro-nano spray needle based on plant hair, comprising the following steps: Step S1, preparing a plant fluff mold by a freezing method.

[0077] The plant fluff mold is prepared by a freezing method, comprising the following steps: Step S1.1, obtaining a plant body with plant hairs, wherein the diameter of the plant hairs is nanometer-level. In this embodiment, the plant body with plant hairs used to prepare the plant hair mold is a creeper leaf 1. The image of the creeper leaf 1 observed by a high-power microscope in the prior art is as follows: Figure 7 As shown, the part similar to the mesh shape is the surface of the leaf; the leaf itself is not completely flat, and there will be micron protrusions, that is, the block-like structure in the picture is the micron protrusion of the leaf; each micron protrusion has a number of plant hairs, that is, the highlighted lines in the picture. However, it is not limited to this. In other embodiments, other plant bodies with plant hairs can be selected according to actual use requirements, and the diameter of the plant hairs includes nanometers.

[0078] Step S1.2, pre-treating the plant body with plant hairs; In step S1.2, the plant body with plant hair is pretreated, including the following steps: Step S1.2.1, placing the plant body with plant hair in deionized water of ultrasonic cleaning machine 2 for ultrasonic cleaning. Specifically, in step S1.2.1, placing the plant body with plant hair in deionized water for ultrasonic cleaning; working time 20min, power 40W, temperature 25°C. More specifically, when ultrasonic cleaning the plant body with plant hair, placing the leaves face down can effectively protect the plant hair and facilitate the discharge of dirt between the hairs.

[0079] Step S1.2.2, drying the plant body with plant hair after ultrasonic cleaning. Specifically, in step S1.2.2, the plant body with plant hair after ultrasonic cleaning is placed in the sun at room temperature for 7 hours to remove moisture on the surface of the plant hair of the plant body and achieve drying.

[0080] Step S1.2.3, trimming the plant body with plant hair after drying. Specifically, in step S1.2.3, a coated tool is used to divide the plant body with plant hair after drying into leaf strips 4 with a width of 8 mm, and leaf strips 4 that meet the preparation requirements are selected to achieve trimming of the plant body with plant hair after drying. More specifically, when selecting the leaf strips 4 of the plant body with plant hair, the leaf strips 4 with hairs on the leaf strips 4 arranged in a single row longitudinally are selected.

[0081] Step S1.3, the plant body with plant hairs processed in step S1.2 is flatly fixed on a glass substrate.

[0082] Step S1.4, freezing the plant body with plant hair fixed on the glass substrate by freezing method, and spraying trimethylchlorosilane on the plant body with plant hair during freezing to obtain a hydrophobic plant hair mold with a certain hardness.

[0083] The freezing method comprises: Step S1.4.1, spraying deionized water mist on the plant body with plant hairs fixed on the glass substrate by using the sprayer 3 to obtain a moist plant body with plant hairs; Step S1.4.2, placing the moist plant body with plant hair in a freezing environment 5 for freezing. Specifically, in step S1.4.2, placing the moist plant body with plant hair in a freezing environment 5 at -23°C for freezing for 4 hours.

[0084] Step S2, using a casting method to cast the plant hair mold to obtain a plant hair-based injection needle model 7 with nanochannels 10.

[0085] In step S2, the casting method comprises the following steps: Step S2.1, the PDMS mixed solution is uniformly flowed from the periphery of the plant fluff mold to the center and covers the plant fluff mold to obtain a PDMS plant fluff mold. Specifically, the preparation method of the PDMS mixed solution in step S2.1 includes: mixing the PDMS base material and the curing agent in a volume ratio of 8:1, stirring for 8 minutes to obtain a PDMS mixed solution, and placing the PDMS mixed solution in a vacuum box for 25 minutes to degas and remove bubbles inside the PDMS mixed solution.

[0086] Step S2.2, placing the PDMS plant hair mold on a leveled horizontal platform for curing. Specifically, step S2.2, placing the PDMS plant hair mold on a leveled horizontal platform for curing at room temperature for 25 hours.

[0087] Step S2.3, separate the PDMS solidified body 6 solidified in the PDMS plant fluff mold from the plant fluff mold to obtain the spray needle model 7 with the nanochannel 10. Specifically, in step S2.3, after separating the PDMS solidified body 6 solidified in the PDMS plant fluff mold from the plant fluff mold, it also includes: placing the nanochannel 10 of the PDMS spray needle model with the nanochannel 10 face down in deionized water for ultrasonic cleaning to remove the residual plant fluff in the nanochannel 10; and placing it on a hot plate 8 for heating to obtain a clean spray needle model 7 with the nanochannel 10. More specifically, in step S2.3, the concave surface of the spray needle model 7 with the nanochannel 10 is placed downward, the power of ultrasonic cleaning is 200W, and the residual plant fluff is removed; the heating temperature for heating on the hot plate 8 is 75°C, and the heating time is 20~30min.

[0088] Step S3, using a laser method to etch a micron channel 11 on the nozzle model 7 with the nanochannel 10 obtained in step S2 to obtain a micro-nano nozzle.

[0089] In step S3, a micron channel 11 is etched on the nozzle model 7 with the nanochannel 10 obtained in step S2 by a laser method to obtain a micro-nano nozzle, which includes the following steps: A clean spray needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 120 μm connected to the nanochannel 10 in the spray needle model is printed one-to-one on the clean spray needle model 7 with a nanochannel 10 by laser etching. Each micron channel 11 is connected to several nanochannels 10 to obtain a PDMS spray needle model with micron channels 11 and nanochannels 10.

[0090] In one embodiment, the micro-nano spray needle obtained in step S3 is a single-channel micro-nano spray needle, comprising the following steps: A clean needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 120 μm connected to the nanochannel 10 in the needle model is printed one-to-one on the clean needle model 7 with a nanochannel 10 by laser etching. Each micron channel 11 is connected to a nanochannel 10, and a single-pass needle model with a micron channel 11 and a nanochannel 10 is obtained; wherein, the printing laser is a carbon dioxide laser, the laser power is 15 W, and the speed is 25 mm / s; with one connected micron channel 11 and nanochannel 10 as a group, the single-pass needle model with the micron channel 11 and the nanochannel 10 is grouped and divided using a coating tool, and a steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit. The steel needle 12 needs to be cleaned in advance and treated with oxygen plasma hydrophilicity before being inserted into the micron channel 11 to improve the bonding strength between the steel needle 12 and the micron channel 11 and prevent ink overflow during printing, so as to obtain a single-channel micro-nano needle (such as Figure 8 as shown).

[0091] In another embodiment, the micro-nano spray needle obtained in step S3 is a multi-channel micro-nano spray needle, comprising the following steps: A clean needle model 7 with a nanochannel 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 120 μm connected to the nanochannel 10 in the needle model is printed one-to-one on the clean needle model 7 with a nanochannel 10 by laser etching, and each micron channel 11 is connected to a nanochannel 10, so as to obtain a multi-channel needle model with micron channels 11 and nanochannels 10; wherein the printing laser is a carbon dioxide laser, the laser power is 15 W, and the speed is 25 mm / s; the multi-channel needle model with micron channels 11 and nanochannels 10 is cut into parts using a coated tool, each part has 3 connected micron channels 11 and nanochannels 10, and a steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit, and the steel needle 12 needs to be cleaned and treated with oxygen plasma hydrophilicity in advance before being inserted into the micron channel 11 to improve the bonding strength between the steel needle 12 and the micron channel 11 and prevent ink overflow during printing, so as to obtain a multi-channel micro-nano needle (such as Fig. 9 as shown).

[0092] In another embodiment, the micro-nano spray needles obtained in step S3 are array micro-nano spray needles, comprising the following steps: A clean needle model 7 with nanochannels 10 is placed on a laser three-dimensional platform, and a micron channel 11 with a diameter of 120 μm connected to the nanochannel 10 in the needle model is printed one-to-one on the clean needle model 7 with nanochannels 10 by laser etching. Each micron channel 11 is connected to three nanochannels 10, and an array needle model with micron channels 11 and nanochannels 10 is obtained, wherein the printing laser is a carbon dioxide laser, the laser power is 25 W, and the speed is 25 mm / s; the array needle model with micron channels 11 and nanochannels 10 is cut into parts using a coated tool, and each part has one micron channel 11 corresponding to three nanochannels 10, and a steel needle 12 treated with oxygen plasma hydrophilicity is inserted into the micron channel 11 for interference fit, and an array micro-nano needle is obtained. The steel needle 12 needs to be cleaned in advance and treated with oxygen plasma hydrophilicity before being inserted into the micron channel 11 to improve the bonding strength between the steel needle 12 and the micron channel 11, and to prevent ink from overflowing during printing, and finally an array micro-nano needle is obtained. Embodiment 4

[0093] On the basis of Example 3, the plant body with plant hairs used to prepare the plant hair mold is a lotus with nano-scale plant hairs. Embodiment 5

[0094] On the basis of the third embodiment, the plant body with plant hairs used for preparing the plant hair mold is a lotus with nano-scale plant hairs. Embodiment 6

[0095] On the basis of Example 3, a micro-nano spray needle based on plant hair is prepared by using a micro-nano spray needle manufacturing method based on plant hair.

[0096] Working principle: The present invention discloses a method for manufacturing a micro-nano spray needle based on plant hairs and a micro-nano spray needle; a PDMS mixed solution is poured on a frozen plant body with plant hairs by a strategy of "surrounding the center" using a freeze casting method, thereby cleverly replicating the hair structure on the surface of the plant leaf of the plant body with plant hairs, and obtaining a nanochannel 10. Next, a laser etching technique is used to etch a micron channel 11 with a diameter above the corresponding nanochannel 10, and a spray needle model 7 with a micron channel 11 and a nanochannel 10 is obtained. Finally, a coated tool is used to cut the spray needle model 7 with a micron channel 11 and a nanochannel 10 as needed, and a steel needle 12 is inserted into the micron channel 11 to obtain a single-channel micro-nano spray needle, a multi-channel micro-nano spray needle, and an array micro-nano spray needle. The present invention has a simple process, low cost, and is easy to realize mass production of spray needles.

[0097] The present invention can obtain large-area nanochannels by three-dimensionally pouring frozen nanoscale plant hairs, and obtain micron channels by laser drilling, and all of them are closed channels. On the basis of obtaining nanochannels, if several spray needles are needed, several micron channels can be laser-drilled, that is, after obtaining nanochannels, a spray needle is obtained. The spray needle array layout can also be cut at will. The process is simple, efficient, and easy to produce in batches.

[0098] The above specific implementation methods are specific support for the scheme ideas proposed in the present invention, and cannot be used to limit the protection scope of the present invention. Any equivalent changes or equivalent modifications made on the basis of this technical scheme in accordance with the technical ideas proposed in the present invention still fall within the scope of protection of the technical scheme of the present invention.

Claims

1. A method for manufacturing a micro-nano spray needle based on plant hair, characterized in that: The following steps are involved: Step S1, preparing a plant fluff mold by a freezing method; Step S2, using a casting method to cast the plant hair mold to obtain a spray needle model with nanochannels based on the plant hair; Step S3, using a laser method to etch a micron channel on the nozzle model with a nanochannel obtained in step S2 to obtain a micro-nano nozzle.

2. The method for manufacturing a micro-nano spray needle based on plant hair according to claim 1, characterized in that: In step S1, a freezing method is used to prepare a plant fluff mold, which includes the following steps: Step S1.1, obtaining a plant body with plant hairs, wherein the cross-sectional diameter of the plant hairs is nanometer-scale; Step S1.2, pre-treating the plant body with plant hairs; Step S1.3, laying the plant body with plant hairs processed in step S1.2 flat and fixing it on a glass substrate; Step S1.4, freezing the plant body with plant hairs fixed on the glass substrate by freezing method, and spraying trimethylchlorosilane on the plant body with plant hairs during freezing to obtain a plant hair mold.

3. The method for manufacturing a micro-nano spray needle based on plant hair according to claim 2, characterized in that: In step S1.2, the plant body with plant hair is pretreated, including the following steps: Step S1.2.1, placing the plant body with plant hairs in deionized water for ultrasonic cleaning; Step S1.2.2, drying the plant body with plant hairs after ultrasonic cleaning; Step S1.2.3, trimming the plant body with plant hair after drying; The freezing method comprises: Step S1.4.1, spraying deionized water mist on the plant body with plant hairs fixed on the glass substrate to obtain a moist plant body with plant hairs; Step S1.4.2, placing the moist plant body with plant hairs in a freezing environment and freezing it.

4. The method for manufacturing a micro-nano spray needle based on plant hair according to claim 3, characterized in that: In step S2, the casting method comprises the following steps: Step S2.1, the PDMS mixed solution is uniformly flowed from the periphery of the plant fluff mold to the center and covers the plant fluff mold to obtain a PDMS plant fluff mold; Step S2.2, placing the PDMS plant hair mold on a leveled platform for curing; Step S2.3, separating the PDMS solidified body in the PDMS plant hair mold from the plant hair mold to obtain a needle model with nanochannels.

5. The method for manufacturing a micro-nano spray needle based on plant hair according to claim 4, characterized in that: In step S3, a micron channel is etched on the nozzle model with a nano-channel obtained in step S2 by a laser method to obtain a micro-nano nozzle, which includes the following steps: A clean nozzle model with nanochannels is placed on a laser three-dimensional platform, and a micron channel with a diameter of 50 to 200 μm connected to the nanochannels in the nozzle model is printed on the clean nozzle model with nanochannels by laser etching. Each micron channel is connected to several nanochannels to obtain a PDMS nozzle model with microchannels and nanochannels.

6. The method for manufacturing a micro-nano spray needle based on plant hair according to claim 5, characterized in that: The micro-nano spray needle obtained in step S3 is a single-channel micro-nano spray needle, which includes the following steps: A clean nozzle model with nanochannels is placed on a laser three-dimensional platform, and a micron channel with a diameter of 50-200 μm connected to the nanochannel in the nozzle model is printed one-to-one on the clean nozzle model with nanochannels by laser etching. Each micron channel is connected to a nanochannel to obtain a single-pass nozzle model with microchannels and nanochannels; wherein the printing laser is a carbon dioxide laser with a laser power of 10-20 W and a speed of 20-30 mm / s; with one connected microchannel and nanochannel as a group, the single-pass nozzle model with microchannels and nanochannels is divided into groups using a coating tool, and a steel needle treated with oxygen plasma hydrophilicity is inserted into the microchannel for interference fit to obtain a single-channel micro-nano nozzle; And / or, the micro-nano spray needle obtained in step S3 is a multi-channel micro-nano spray needle, comprising the following steps: A clean nozzle model with nanochannels is placed on a laser three-dimensional platform, and a micron channel with a diameter of 50-200 μm connected to the nanochannel in the nozzle model is printed one-to-one on the clean nozzle model with nanochannels by laser etching, and each micron channel is connected to a nanochannel to obtain a multi-channel nozzle model with micron channels and nanochannels; wherein the printing laser is a carbon dioxide laser, the laser power is 10-20 W, and the speed is 20-30 mm / s; the multi-channel nozzle model with micron channels and nanochannels is cut into parts using a coated tool, each part has 2-4 connected micron channels and nanochannels, and a steel needle treated with oxygen plasma hydrophilicity is inserted into the micron channel for interference fit to obtain a multi-channel micro-nano nozzle; And / or, the micro-nano spray needles obtained in step S3 are array micro-nano spray needles, comprising the following steps: A clean needle spray model with nanochannels was placed on a laser three-dimensional platform, and micron channels with a diameter of 50-200 μm connected to the nanochannels in the needle spray model were printed one-to-one on the clean needle spray model with nanochannels by laser etching. Each micron channel was connected to 2-4 nanochannels, and an array needle spray model with micron and nanochannels was obtained. The printing laser was a carbon dioxide laser with a laser power of 20-30 W and a speed of 20-30 mm / s. The array needle spray model with micron and nanochannels was cut into parts using a coated tool, and each part had a micron channel connected to 2-4 nanochannels, and a steel needle treated with oxygen plasma hydrophilicity was inserted into the micron channel for interference fit to obtain an array micro-nano needle spray.

7. The method for manufacturing a micro-nano spray needle based on plant hair according to claim 6, characterized in that: In step S1.2.1, the plant body with plant hairs is placed in deionized water for ultrasonic cleaning; the working time is 15-25 minutes, the power is 20-60W, and the temperature is 20-30°C; And / or, in step S1.2.2, the plant body with plant hairs after ultrasonic cleaning is placed in the sun for 6 to 8 hours at room temperature to remove moisture on the surface of the plant hairs of the plant body and achieve drying; And / or, in step S1.2.3, the plant body with plant hairs after drying is cut into leaf strips with a width of 5 to 10 mm using a coated cutter, and leaf strips that meet the preparation requirements are selected to trim the plant body with plant hairs after drying; And / or, in step S1.4.2, the moist plant body with plant hairs is placed in a freezing environment at -30 to -15°C for 3 to 5 hours.

8. The method for manufacturing a micro-nano spray needle based on plant hair according to claim 7, characterized in that: The preparation method of the PDMS mixed solution in step S2.1 includes: mixing a PDMS base material and a curing agent in a volume ratio of 5:1 to 10:1, stirring for 5 to 10 minutes to obtain a PDMS mixed solution, and placing the PDMS mixed solution in a vacuum box for 20 to 30 minutes to degas and remove bubbles inside the PDMS mixed solution; and / or, step S2.2, placing the PDMS plant hair mold on a leveled platform and curing it at room temperature for 18 to 32 hours; And / or, in step S2.3, after the PDMS solidified body in the PDMS plant hair mold is separated from the plant hair mold, the method further includes: placing the nanochannel side of the PDMS needle model with nanochannels downward in deionized water for ultrasonic cleaning to remove the residual plant hair in the nanochannels; and placing it on a hot plate for heating to obtain a clean needle model with nanochannels.

9. The method for manufacturing a micro-nano spray needle based on plant hair according to claim 8, characterized in that: The plant body with plant fluff used for preparing the plant fluff mold includes one of creeper leaves, lotus and lotus; And / or, when ultrasonically cleaning a plant body with plant hairs, placing the leaves face down can effectively protect the plant hairs and facilitate the discharge of dirt between the hairs; and / or, when selecting leaf strips of a plant body with plant hairs, selecting leaf strips in which hairs on the leaf strips are arranged longitudinally in a single row; and / or, in step S2.3, the needle model with the nanochannel is placed with the concave surface facing downward, and the power of ultrasonic cleaning is 100-300W to remove the residual plant hair; And / or, in step S2.3, the heating temperature of the heating on the hot plate is 60-90° C., and the heating time is 20-30 min.

10. A micro-nano spray needle based on plant hair, characterized by: The micro-nano spray needle is prepared by the method for manufacturing a micro-nano spray needle based on plant hair as described in any one of claims 1 to 9.

Images