Antibacterial non-woven fabric containing bacillus and preparation method thereof

Abstract

The invention discloses an antibacterial non-woven fabric containing bacillus and a preparation method of the antibacterial non-woven fabric. The antibacterial non-woven fabric comprises a non-woven fabric matrix and an antibacterial coating loaded on the non-woven fabric matrix, the raw materials of the antibacterial coating comprise soybean extract and bacillus, and the pH value of the antibacterial coating is smaller than or equal to 4 or larger than or equal to 9. The bacillus and the soybean extract are added into the coating, and the bacillus can generate lysozyme and antibacterial peptide, so that the non-woven fabric has antibacterial and antiviral effects; and the bacillus can form spores at high temperature, high pressure or extreme pH, and can continuously exert activity after being cultured under proper conditions, so that the heat resistance, acid and alkali resistance and service life of the antibacterial non-woven fabric are improved.

Description

Technical Field

[0001] The invention relates to the technical field of antibacterial nonwoven fabrics, and in particular to an antibacterial nonwoven fabric containing Bacillus and a preparation method thereof. Background Art

[0002] Lysozyme is an alkaline enzyme that can hydrolyze mucopolysaccharides in pathogenic bacteria. It mainly breaks down the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall, decomposing the insoluble mucopolysaccharides in the cell wall into soluble glycopeptides, causing the cell wall to rupture and the contents to escape, thereby dissolving the bacteria. Lysozyme can also directly bind to negatively charged viral proteins, forming complex salts with DNA, RNA, and apoproteins, inactivating the virus. Therefore, the enzyme has antibacterial, anti-inflammatory, and antiviral effects.

[0003] Taking advantage of the characteristics of lysozyme, there is currently research on fixing it on fibers to develop fabrics with antibacterial properties. For example, Liu Xianming et al. fixed lysozyme on HEPA air filtration high-efficiency filter materials made of artificial fibers to produce biological sterilization filter materials, and used them as air purification systems for air conditioners to develop industrial textiles with high-efficiency dust removal and sterilization functions (Liu Xianming, Ma Yunjun. HEPA containing lysozyme and dual sterilization filtration technology [J]. Household Appliance Technology, 2001 (2): 20-21). However, enzymes are easily inactivated by heat, so the durability and service life of antibacterial textiles made with lysozyme are relatively short.

[0004] Patent WO2011055893A1 discloses an air filter, which has a protein deactivator, kimchi lactic acid bacteria and a disinfectant coated and fixed on a carrier, thereby effectively cleaning the air by removing or killing bacteria, fungi and viruses in the air. However, the product in this patent also has poor heat resistance. If it is exposed to high temperatures for a long time during use and transportation, it will lose its antibacterial effect.

[0005] Patent JP2024100833A discloses a non-woven fabric that uses spores to inhibit the growth of pathogens. Since the bacteria are in the spore state, it takes several days for the non-woven fabric to activate its full antibacterial potential, which is not conducive to practical application. Summary of the invention

[0006] The present invention aims to overcome the above-mentioned problems existing in the antibacterial fabrics to which lysozyme or microorganisms or spores are added in the prior art, and provides an antibacterial non-woven fabric containing Bacillus and a preparation method thereof. Bacillus and soybean extract are added to the coating, and the Bacillus can produce lysozyme and antimicrobial peptides, so that the non-woven fabric has antibacterial and antiviral effects; and under high temperature, high pressure or extreme pH, the Bacillus can form spores, which can continue to exert their activity after being cultured under suitable conditions, thereby improving the heat resistance, acid and alkali resistance and service life of the antibacterial non-woven fabric.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions: An antibacterial non-woven fabric containing bacillus comprises a non-woven fabric substrate and an antibacterial coating loaded on the non-woven fabric substrate; the raw materials of the antibacterial coating comprise soybean extract and bacillus liquid, and the pH value of the antibacterial coating is ≤4 or ≥9.

[0008] The present invention adds bacillus and soybean extract to the coating, and the soybean extract can provide nutrients for the growth of bacillus. Bacillus can secrete lysozyme and antimicrobial peptides during the growth and metabolism process, so that the non-woven fabric has antibacterial and antiviral effects. Moreover, under high temperature, high pressure or extreme pH, bacillus can form spores and will not die. Even if active ingredients such as lysozyme are temporarily inactivated, bacillus can continue to produce active ingredients after cultivation under suitable conditions. Compared with the existing non-woven fabrics directly loaded with lysozyme, the present invention can significantly improve the high temperature resistance and acid and alkali resistance of the antibacterial non-woven fabric. Moreover, when the present invention is used in an environment of non-high temperature, high pressure or extreme pH, bacillus can continue to produce lysozyme and antimicrobial peptides, and can immediately exert antibacterial effects without the need for a long activation time.

[0009] At the same time, the present invention adjusts the pH value of the antibacterial coating to below 4 or above 9, which can slow down the growth of Bacillus, so that the antibacterial performance of the non-woven fabric can be maintained for a long time, and its service life can be extended. When the antibacterial non-woven fabric of the present invention is used as a filter material in a filter, the Bacillus can also decompose dust and other organic matter adsorbed on the surface of the non-woven fabric, thereby reducing the replacement frequency of the filter material.

[0010] Preferably, the mass volume ratio of the added soybean extract and the Bacillus solution is 15-25 g:1 mL; the concentration of the Bacillus solution is 10 3 ~10 5 CFU / mL.

[0011] Preferably, the non-woven fabric substrate is one of PP non-woven fabric, PE non-woven fabric and PET non-woven fabric.

[0012] The present invention also discloses a method for preparing the antibacterial non-woven fabric containing Bacillus, comprising the following steps: (1) adding soybean extract to water, then adding Bacillus liquid, stirring and adjusting the pH value of the system to ≤4 or ≥9 to obtain a coating solution; (2) The non-woven fabric substrate is immersed in the coating solution and then taken out and dried to obtain the antibacterial non-woven fabric containing Bacillus.

[0013] Preferably, in the coating solution obtained in step (1), the mass volume ratio of soybean extract to water is 15-25 g:1000 mL.

[0014] Preferably, after adding the soybean extract to water in step (1), high temperature sterilization is first performed, and then the Bacillus liquid is added; the high temperature sterilization temperature is 100-130° C. and the sterilization time is 10-15 min.

[0015] Preferably, after adding the Bacillus liquid in step (1), stirring is carried out at a temperature of 60 to 70° C. for 12 to 24 hours.

[0016] Preferably, in step (2), the non-woven fabric substrate is immersed in the coating solution for a time of ≥ 2 min.

[0017] Preferably, in step (2), after the soaked nonwoven fabric is taken out from the coating solution, it is placed at room temperature for 30 to 60 minutes and then dried.

[0018] Preferably, the drying temperature in step (2) is 65-75° C., and the drying time is 40-80 min.

[0019] Therefore, the present invention has the following beneficial effects: (1) The lysozyme and antimicrobial peptides secreted by Bacillus during its growth and metabolism can make non-woven fabrics have antibacterial and antiviral effects; (2) Under high temperature, high pressure or extreme pH, Bacillus can form spores and will not die. Even if active ingredients such as lysozyme are temporarily inactivated, Bacillus can continue to produce active ingredients after cultivation under suitable conditions, thereby improving the high temperature resistance and acid and alkali resistance of the antibacterial non-woven fabric; (3) When used in an environment that is not subjected to high temperature, high pressure or extreme pH, Bacillus can continuously produce lysozyme and antimicrobial peptides, which can immediately exert antimicrobial effects without the need for a long activation time; (4) Adjusting the pH value of the antibacterial coating to below 4 or above 9 can slow down the growth of Bacillus, so that the antibacterial properties of the nonwoven fabric can be maintained for a long time, extending its service life; (5) When the antibacterial nonwoven fabric of the present invention is used as a filter material in a filter, Bacillus can also decompose dust and other organic matter adsorbed on the surface of the nonwoven fabric, thereby reducing the frequency of replacement of the filter material. DETAILED DESCRIPTION

[0020] The present invention is further described below in conjunction with specific implementation methods.

[0021] In the present invention, unless otherwise specified, all equipment and raw materials can be purchased from the market or are commonly used in the industry. The methods in the following embodiments, unless otherwise specified, are conventional methods in the art.

[0022] In order to further understand the present invention, the preferred embodiments of the present invention are described below in conjunction with specific implementation methods, but it should be understood that these descriptions are only to further illustrate the features and advantages of the present invention rather than to limit the claims of the present invention.

[0023] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0024] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0025] Overall embodiment: An antibacterial non-woven fabric containing bacillus comprises a non-woven fabric substrate and an antibacterial coating loaded on the non-woven fabric substrate; the raw materials of the antibacterial coating comprise soybean extract and bacillus liquid, and the pH value of the antibacterial coating is ≤4 or ≥9.

[0026] As a specific embodiment, the mass volume ratio of the added soybean extract and the Bacillus liquid is 15-25g:1mL; the concentration of the Bacillus liquid is 10 3 ~10 5 CFU / mL.

[0027] As a specific implementation, the non-woven fabric substrate is one of PP non-woven fabric, PE non-woven fabric, and PET non-woven fabric.

[0028] The method for preparing the antibacterial nonwoven fabric containing Bacillus comprises the following steps: (1) adding soybean extract to water, then adding Bacillus liquid, stirring and adjusting the pH value of the system to ≤4 or ≥9 to obtain a coating solution; (2) The non-woven fabric substrate is immersed in the coating solution and then taken out and dried to obtain the antibacterial non-woven fabric containing Bacillus.

[0029] As a specific implementation, in the coating solution obtained in step (1), the mass volume ratio of soybean extract to water is 15-25g:1000mL.

[0030] As a specific implementation, after adding the soybean extract to water in step (1), high temperature sterilization is first performed, and then the Bacillus liquid is added; the high temperature sterilization temperature is 100-130° C. and the sterilization time is 10-15 minutes.

[0031] As a specific implementation, after adding the Bacillus liquid in step (1), stirring is carried out at a temperature of 60 to 70° C. for 12 to 24 hours.

[0032] As a specific implementation manner, in step (2), the non-woven fabric substrate is immersed in the coating solution for a time of ≥2 min.

[0033] As a specific implementation, in step (2), after the non-woven fabric is taken out from the coating solution after being immersed, it is first placed at room temperature for 30 to 60 minutes and then dried.

[0034] As a specific implementation, the drying temperature in step (2) is 65-75° C., and the drying time is 40-80 min.

[0035] Embodiment 1: A method for preparing an antibacterial nonwoven fabric containing Bacillus, comprising the following steps: (1) 20 g of soybean extract was added to 1000 mL of water, and the mixture was sterilized at 121°C for 15 min. Then 1 mL of Bacillus subtilis solution (10 4 CFU / mL), stirred at 60°C for 16h, then hydrochloric acid was added to adjust the pH value of the system to 4.0 to obtain a coating solution; (2) soaking the PP nonwoven fabric in the coating solution obtained above for 2 minutes; taking the soaked PP nonwoven fabric out of the coating solution, leaving it at room temperature for 45 minutes, and then drying it at 70° C. for 60 minutes to obtain the antibacterial nonwoven fabric containing Bacillus.

[0036] Embodiment 2: A method for preparing an antibacterial nonwoven fabric containing Bacillus, comprising the following steps: (1) 20 g of soybean extract was added to 1000 mL of water, and then autoclaved at 110 °C for 10 min. Then 1 mL of Bacillus subtilis solution (10 4 CFU / mL), stirred at 65°C for 18h, then hydrochloric acid was added to adjust the pH value of the system to 4.0 to obtain a coating solution; (2) soaking the PP nonwoven fabric in the coating solution obtained above for 2 minutes; taking the soaked PP nonwoven fabric out of the coating solution, leaving it at room temperature for 60 minutes, and then drying it at 70° C. for 60 minutes to obtain the antibacterial nonwoven fabric containing Bacillus.

[0037] Embodiment 3: A method for preparing an antibacterial nonwoven fabric containing Bacillus, comprising the following steps: (1) 20 g of soybean extract was added to 1000 mL of water, and then sterilized at 100 °C for 10 min. Then 1 mL of Bacillus subtilis solution (10 4 CFU / mL), stirred at 70°C for 24h, and then added sodium hydroxide solution to adjust the pH value of the system to 9.0 to obtain a coating solution; (2) soaking the PP nonwoven fabric in the coating solution obtained above for 2 minutes; taking the soaked PP nonwoven fabric out of the coating solution, leaving it at room temperature for 30 minutes, and then drying it at 70° C. for 60 minutes to obtain the antibacterial nonwoven fabric containing Bacillus.

[0038] Embodiment 4: A method for preparing an antibacterial nonwoven fabric containing Bacillus, comprising the following steps: (1) 20 g of soybean extract was added to 1000 mL of water, and the mixture was sterilized at 120 °C for 10 min. Then 1 mL of Bacillus subtilis solution (10 4 CFU / mL), stirred at 70°C for 24h, and then hydrochloric acid was added to adjust the pH value of the system to 4.0 to obtain a coating solution; (2) soaking the PP nonwoven fabric in the coating solution obtained above for 2 minutes; taking the soaked PP nonwoven fabric out of the coating solution, leaving it at room temperature for 30 minutes, and then drying it at 70° C. for 60 minutes to obtain the antibacterial nonwoven fabric containing Bacillus.

[0039] Comparative Example 1: A method for preparing an antibacterial nonwoven fabric containing lysozyme comprises the following steps: (1) 1 g of lysozyme was added to 1000 mL of water, stirred at 60° C. for 16 h, and then hydrochloric acid was added to adjust the pH value of the system to 4.0 to obtain a coating solution; (2) soaking the PP nonwoven fabric in the coating solution obtained above for 2 minutes; taking the soaked PP nonwoven fabric out of the coating solution, leaving it at room temperature for 45 minutes, and then drying it at 70° C. for 60 minutes to obtain the antibacterial nonwoven fabric containing lysozyme.

[0040] Comparative Example 2: A method for preparing an antibacterial nonwoven fabric containing Bacillus, comprising the following steps: (1) 20 g of soybean extract was added to 1000 mL of water, and the mixture was sterilized at 121°C for 15 min. Then 1 mL of Bacillus subtilis solution (10 4CFU / mL), stirred at 60°C for 16 h, and then the pH value of the system was adjusted to 7.0 to obtain a coating solution; (2) soaking the PP nonwoven fabric in the coating solution obtained above for 2 minutes; taking the soaked PP nonwoven fabric out of the coating solution, leaving it at room temperature for 45 minutes, and then drying it at 70° C. for 60 minutes to obtain the antibacterial nonwoven fabric containing Bacillus.

[0041] Test Example 1: The antibacterial nonwoven fabric containing Bacillus obtained in Example 1 was heated at 90°C for 30 minutes and then placed at 25°C for 24 hours. Its bacteriolytic activity, antiviral performance, antiallergic performance and antifungal effect were tested. The results are shown in Table 1.

[0042] Test Example 2: The surface of the antibacterial non-woven fabric containing Bacillus obtained in Example 2 was sprayed with 2 mL of hydrochloric acid solution with a pH value of 3, and then placed at 25° C. for 24 hours. The bacteriolytic activity, antiviral performance, antiallergic performance and anti-mildew effect were tested. The results are shown in Table 1.

[0043] Test Example 3: The surface of the antibacterial non-woven fabric containing Bacillus obtained in Example 3 was sprayed with 2 mL of sodium hydroxide solution with a pH value of 12, and then placed at 25° C. for 24 hours. The bacteriolytic activity, antiviral performance, antiallergic performance and anti-mildew effect were tested. The results are shown in Table 1.

[0044] Test Example 4: The antibacterial nonwoven fabric containing Bacillus obtained in Example 4 was placed at 25° C. for one year, and its bacteriolytic activity, antiviral performance, antiallergic performance and antifungal effect were tested. The results are shown in Table 1.

[0045] Comparative test example 1: The antibacterial nonwoven fabric containing lysozyme obtained in Comparative Example 1 was heated at 90°C for 30 min and then placed at 25°C for 24 h. Its bacteriolytic activity, antiviral performance, antiallergic performance and antifungal effect were tested. The results are shown in Table 1.

[0046] Comparative test example 2: The surface of the antibacterial non-woven fabric containing lysozyme obtained in Comparative Example 1 was sprayed with 2 mL of hydrochloric acid solution with a pH value of 3, and then placed at 25° C. for 24 hours. The bacteriolytic activity, antiviral performance, antiallergic performance and anti-mildew effect were tested. The results are shown in Table 1.

[0047] Comparative test example 3: The surface of the antibacterial non-woven fabric containing lysozyme obtained in Comparative Example 1 was sprayed with 2 mL of sodium hydroxide solution with a pH value of 12, and then placed at 25° C. for 24 hours. The bacteriolytic activity, antiviral performance, antiallergic performance and anti-mildew effect were tested. The results are shown in Table 1.

[0048] Comparative test example 4: The antibacterial nonwoven fabric containing lysozyme obtained in Comparative Example 1 was placed at 25° C. for one year, and its bacteriolytic activity, antiviral performance, antiallergic performance and antifungal effect were tested. The results are shown in Table 1.

[0049] Comparative test example 5: The antibacterial nonwoven fabric containing Bacillus obtained in Comparative Example 2 was placed at 25° C. for one year, and its bacteriolytic activity, antiviral performance, antiallergic performance and antifungal effect were tested. The results are shown in Table 1.

[0050] The lytic activity test method is as follows: the non-woven fabric obtained in each embodiment and comparative example is cut into samples of 1 square centimeter, and then freeze-dried Micrococcus luteus particles are sprinkled on the samples, and then the samples are inverted to remove the boundary particles, and then the degree of lysis of Micrococcus luteus is observed with a low vacuum scanning electron microscope; if the spherical shape of the bacteria has been decomposed, it is determined that the sample has lytic activity; if the spherical shape still exists, it is determined that the sample does not have lytic activity.

[0051] The test method for antiviral performance is as follows: according to ISO 18184 standard, the nonwoven fabric obtained in each embodiment and comparative example is cut into 33 square millimeter samples; the samples are placed in a sealed container and 0.1 mL of a titer of 10 8 PFU / mL of influenza virus (InfluenzavirusA PR / 8) aqueous solution was inoculated into each sample, and then reacted at room temperature for 2 hours; the sample was rinsed with 10mL SCDLP solution, and the collected solution was inoculated into MDCK cells through a 0.22μm filter, incubated at 34°C for 48 hours, and then the influenza virus infection titer of the plaque was measured, and its antiviral activity value Mv was calculated. When 3.0>Mv≥2.0, the surface has an antiviral effect; when Mv≥3.0, it indicates a good antiviral effect.

[0052] The anti-allergic performance test method is as follows: the non-woven fabric obtained in each embodiment and comparative example is cut into a sample of 1 square centimeter, placed in a mite allergen (Derf2) solution (concentration: 50 ng / mL, volume: 200 μL), left to stand for 24 hours, then the sample is taken out and the allergen concentration in the solution is measured.

[0053] The anti-mildew effect test method is: according to JIS Z 2911 (anti-mildew test method), an anti-mildew test is performed on Aspergillus niger.

[0054] Table 1: Test results. serial number Lytic activity Antiviral activity value Allergen concentration Mildew resistance test Test Example 1 Bacteriolytic activity ＞3.0 <![CDATA[<1μg / m 2 ]]> No mold was found Test Example 2 Has bacteriolytic activity ＞3.0 <![CDATA[<1μg / m 2 ]]> No mold was found Test Example 3 Has bacteriolytic activity ＞3.0 <![CDATA[<1μg / m 2 ]]> No mold was found Test Example 4 Has bacteriolytic activity ＞3.0 <![CDATA[<1μg / m 2 ]]> No mold was found Comparative test example 1 No bacteriolytic activity 0 <![CDATA[>35μg / m 2 ]]> There is mold Comparative test example 2 No bacteriolytic activity 0 <![CDATA[>35μg / m 2 ]]> There is mold Comparative test example 3 No bacteriolytic activity 0 <![CDATA[>35μg / m 2 ]]> There is mold Comparative test example 4 No bacteriolytic activity 0 <![CDATA[>35μg / m 2 ]]> There is mold Comparative test example 5 No bacteriolytic activity 0 <![CDATA[>35μg / m 2 ]]> There is mold

[0055] It can be seen from the data in Table 1 that the nonwoven fabrics prepared by the method of the present invention in Examples 1 to 4 have excellent antibacterial, antiviral, antiallergic and antifungal properties. It can be seen from the results of Test Example 4 that the nonwoven fabric can still maintain excellent antibacterial, antiviral, antiallergic and antifungal properties after one year by adjusting the pH value of the coating solution to 4. The nonwoven fabric of the present invention can effectively inactivate various bacteria, molds, viruses and allergenic substances remaining in the nonwoven fabric through enzymes and peptides produced by Bacillus, and its ability will not change due to heating or pH changes; this ability can be reflected immediately after use without the need for a long activation time.

[0056] It can be seen from the results of Comparative Test Examples 1 to 4 that in Comparative Example 1, lysozyme is directly loaded on the non-woven fabric, and its heat resistance, acid and alkali resistance and service life are all reduced compared with those of the present invention. It no longer has antibacterial, antiviral, anti-allergic and anti-mildew properties under high temperature, strong acid and alkali and after being placed for one year.

[0057] From the results of comparative test example 5, it can be seen that in comparative example 2, the pH value of the coating solution was adjusted to 7, and the non-woven fabric no longer had antibacterial, antiviral, antiallergic and antifungal properties after being placed for one year. This shows that the present invention can make Bacillus grow slowly and maintain its performance for a long time by adjusting the pH value of the coating solution to ≤4 or pH value ≥9.

[0058] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. An antibacterial nonwoven fabric containing Bacillus, characterized in that: The invention comprises a non-woven fabric substrate and an antibacterial coating loaded on the non-woven fabric substrate; the raw materials of the antibacterial coating comprise soybean extract and bacillus liquid, and the pH value of the antibacterial coating is ≤4 or ≥9.

2. The antibacterial nonwoven fabric containing Bacillus according to claim 1, characterized in that: The mass volume ratio of the added soybean extract and Bacillus solution was 15-25 g:1 mL; the concentration of the Bacillus solution was 10 3 ~10 5 CFU / mL.

3. The antibacterial nonwoven fabric containing Bacillus according to claim 2, characterized in that: The non-woven fabric substrate is one of PP non-woven fabric, PE non-woven fabric and PET non-woven fabric.

4. A method for preparing an antibacterial nonwoven fabric containing Bacillus as claimed in any one of claims 1 to 3, characterized in that: The steps include: (1) adding soybean extract into water, then adding Bacillus liquid, stirring and adjusting the pH value of the system to ≤4 or ≥9 to obtain a coating solution; (2) The non-woven fabric substrate is immersed in the coating solution, taken out and dried to obtain the antibacterial non-woven fabric containing Bacillus.

5. The method for preparing the antibacterial nonwoven fabric containing Bacillus according to claim 4, characterized in that: In the coating solution obtained in step (1), the mass volume ratio of soybean extract to water is 15-25 g:1000 mL.

6. The method for preparing the antibacterial nonwoven fabric containing Bacillus according to claim 4, characterized in that: In step (1), after adding the soybean extract into water, high temperature sterilization is first performed, and then the Bacillus liquid is added; the high temperature sterilization temperature is 100-130° C. and the sterilization time is 10-15 minutes.

7. The method for preparing the antibacterial nonwoven fabric containing Bacillus according to claim 4 or 6, characterized in that: After adding the Bacillus liquid in step (1), stirring is carried out at a temperature of 60-70° C. for 12-24 hours.

8. The method for preparing the antibacterial nonwoven fabric containing Bacillus according to claim 4, characterized in that: In step (2), the non-woven fabric substrate is immersed in the coating solution for ≥ 2 min.

9. The method for preparing an antibacterial nonwoven fabric containing Bacillus according to claim 4, characterized in that: In step (2), after the soaked non-woven fabric is taken out from the coating solution, it is placed at room temperature for 30 to 60 minutes and then dried.

10. The method for preparing the antibacterial nonwoven fabric containing Bacillus according to claim 4 or 9, characterized in that: The drying temperature in step (2) is 65-75°C, and the drying time is 40-80 minutes.