Treatment method for promoting sterile germination of corn seeds

Abstract

The invention belongs to the technical field of plant propagation, and particularly relates to a treatment method for promoting sterile germination of corn seeds, which comprises the following steps: taking corn seeds, and disinfecting the seeds; soaking the disinfected corn seeds in sterile water at room temperature for 6-8 hours; the method comprises the following steps: firstly, disinfecting a corn seed germination device, and uniformly placing soaked corn seeds in the corn seed germination device; then sterile water is added into the corn seed germination device, so that the corn seed germination device absorbs and retains water capable of enabling the corn seeds to germinate, and finally, the corn seed germination device is placed in an environment at the temperature of 24-26 DEG C for dark culture until the corn seeds germinate; the disinfection method comprises the following steps: soaking and disinfecting for 15-20 minutes by using a sodium hypochlorite solution with the mass fraction of 0.9-1.1%, and rinsing by using a sterile osmotic regulator. The corn seed disinfection agent not only achieves a disinfection effect, but also achieves an osmotic regulation effect and an effect of promoting sterile germination of corn seeds.

Description

Technical Field

[0001] The invention belongs to the technical field of plant propagation, and in particular relates to a treatment method for promoting aseptic germination of corn seeds. Background Art

[0002] Maize (Zea mays L.), an annual herbaceous plant of the genus Zea in the family Poaceae, is one of the most important crops and is the main cereal crop grown in temperate and tropical regions under a wide range of soil and climatic conditions. Maize is not only consumed directly in the human diet, but is also widely used in biofuels, animal feed, and various industrial products, including syrup and corn starch. Maize is a C4-cycling grass and a crop species with moderate sensitivity to abiotic stresses such as drought and temperature. Due to its use in different ecosystems, the maize crop faces a wide range of environmental abiotic stresses, including drought and rising temperatures due to climate change, which leads to significant yield losses. These abiotic stresses and soil environmental conditions may reduce maize seed germination rates while causing adaptation to induced stresses. Germination affects the final yield and quality of maize. Germination is controlled by the interaction of environmental factors, maize seed physiological state, and embryo. The requirements of maize for various abiotic factors mainly depend on these surrounding abiotic factors and maize genotype. Maize germination requires favorable environmental conditions, and a specific range of these factors is necessary for optimal germination. Conditions such as temperature, light and water availability can affect germination.

[0003] In the prior art, there are two factors that affect corn seed germination: one is internal factors, and the other is external factors. Internal factors mainly include complete living embryos and nutrients required for embryo growth; external factors include water, oxygen, suitable temperature and light, including soaking time and osmotic regulator concentration. Among them, although osmotic regulation can regulate the physiological metabolism of corn seeds, corn belongs to higher plants, and the germination of its corn seeds is also controlled by multiple genes and metabolic pathways. Once the osmotic regulation method used is improper, it will conflict with the control of genes and metabolic pathways, affecting the germination of corn seeds. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a treatment method for promoting the sterile germination of corn seeds, which avoids conflicts with the control of genes and metabolic pathways during the osmotic adjustment process, thereby promoting the germination of corn seeds well.

[0005] The object of the present invention is to provide a treatment method for promoting aseptic germination of corn seeds, comprising:

[0006] Take corn seeds and sterilize them;

[0007] Add the sterilized corn seeds into sterile water and soak them at room temperature for 6 to 8 hours;

[0008] The corn seed germination device is sterilized, and the soaked corn seeds are placed in the corn seed germination device; sterile water is added into the corn seed germination device so that the corn seed germination device absorbs and retains water that can make the corn seeds germinate; finally, the corn seed germination device is placed in an environment of 24° C. to 26° C. for dark culture until the corn seeds germinate;

[0009] The method of seed disinfection is: soak corn seeds in 0.9% to 1.1% sodium hypochlorite solution, disinfect for 15 to 20 minutes, and then rinse with a sterile osmotic regulator;

[0010] The sterile osmotic regulator is a 3g / L to 12g / L sterile potassium sorbate solution or a 0.05g / L to 0.1g / L sterile lead nitrate solution, or a combination of either of the two and sterile water.

[0011] 3g / L~12g / L is a high concentration of sterile potassium sorbate solution, and 0.05g / L~0.1g / L is a low concentration of sterile lead nitrate solution, both of which have the effect of promoting corn seed germination. The combination of either of the two with sterile water can also play a role in promoting corn seed germination.

[0012] Preferably, the temperature of the sterile osmotic regulator is 45° C. to 48° C. After treatment at this temperature, the germination rate of corn seeds is higher.

[0013] Preferably, the pH of the sterile osmotic regulator is 4.0 to 6.0.

[0014] Preferably, the seeds are rinsed with a sterile osmotic regulator for 3 to 5 times, and in order to reduce the residual potassium sorbate or lead nitrate on the surface of the corn seeds, the sterile osmotic regulator used for the last time is sterile water. The last use of sterile water not only promotes corn seed germination, but also washes away the potassium sorbate or lead nitrate remaining on the surface of the corn seeds to ensure that the potassium sorbate or lead nitrate is used within the specified time.

[0015] Preferably, the sterile osmotic regulator method is: soak the sterilized corn seeds in a 0.9% to 1.1% sodium hypochlorite solution, rinse them in the sterile osmotic regulator solution for 1 to 2 minutes, and then drain the water. Draining the water means that there is no water drop on the surface of the corn seeds.

[0016] Preferably, room temperature refers to 20°C to 25°C.

[0017] Preferably, the corn seed germination device is a corn seed germination bag, which includes a transparent bag and a paper core located inside the transparent bag. The soaked corn seeds are placed in the paper core, and then sterile water is added to the paper core to allow the paper core to fully absorb and retain moisture that can make the corn seeds germinate.

[0018] Preferably, the size of the corn seed germination bag is 14 cm to 18 cm in length and 12.5 cm to 14 cm in width.

[0019] Preferably, 10 to 30 soaked corn seeds are placed in each corn seed germination bag.

[0020] Preferably, the amount of water used to fully soak the paper core and retain enough water to make the corn seeds germinate is 40 mL to 60 mL.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The treatment method for promoting aseptic germination of corn seeds provided by the present invention comprises the steps of taking corn seeds, disinfecting, soaking seeds at room temperature for 6h to 8h, germination, etc. Among them, the method of disinfection is: soaking corn seeds with a sodium hypochlorite solution of 0.9% to 1.1% by mass, disinfecting for 15min to 20min, and rinsing with a sterile osmotic regulator, wherein the sterile osmotic regulator is a sterile potassium sorbate solution of 3g / L to 12g / L or a sterile lead nitrate solution of 0.05g / L to 0.1g / L, or a combination of either of the two and sterile water. On the one hand, the osmotic regulator can regulate the physiological metabolism of corn seeds, increase the utilization of materials and energy by corn seeds, thereby enhancing the vitality of corn seeds, and is an effective induction method for promoting the germination of corn seeds; on the other hand, soaking seeds with an osmotic regulator can break the dormancy of corn seeds faster, promote the growth of embryos, shorten the cultivation cycle, and improve the germination quality of corn seeds as a whole. Furthermore, in the present invention, the sterile osmotic regulator is sterile, will not cause corn seeds to be infected with foreign bacteria, and will not be interfered with by corn genotype and metabolic activity, thus avoiding conflicts with the control of genes and metabolic pathways, thereby promoting corn seed germination well.

[0023] Furthermore, the present invention uses sterile water to soak seeds at room temperature of 20°C to 25°C for 6h to 8h, which is a sterile germination condition for soaking corn seeds. On the one hand, it can make the seed coat swell, increase the permeability of the seed coat, and facilitate the entry of exogenous substances. On the other hand, it improves the germination rate of corn seeds and overcomes the problem of corn seed germination dormancy.

[0024] In summary, the method of the present invention ensures that the contamination rate of corn seeds during the aseptic germination process is low, and overcomes the problem of easy contamination during the aseptic germination process due to the small number of corn seeds and the large number of microorganisms in the traditional germination of corn seeds. In addition, while ensuring the low contamination rate of corn seeds, it avoids conflicts with the control of genes and metabolic pathways, and the aseptic germination rate of corn seeds is greatly improved. In addition, the corn seed germination device used in the present invention is simple, easy to operate, and easy to carry out small space aseptic corn seedling production. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a physical picture of the germination process of corn seeds. DETAILED DESCRIPTION

[0026] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below in conjunction with specific embodiments and drawings.

[0027] According to the research results of the prior art, potassium sorbate has an inhibitory effect on microorganisms and can be used as an antibacterial agent. However, the actual research results show that potassium sorbate inhibits the germination potential and germination rate of corn seeds while inhibiting bacteria. See "Hu Chunhong, Shi Liugong, Li Shumei, et al. Effects of preservative seed soaking on corn seed germination and seedling growth [J]. Henan Agricultural Science, 2012, 41(5):3. DOI:10.3969 / j.issn.1004-3268.2012.05.005". The reason why corn seed germination is inhibited is that potassium sorbate conflicts with corn genotype and metabolic activity, which will cause an increase in the micronucleus rate of plant root tip cells. The present invention also found that another reason why potassium sorbate inhibits corn seed germination is that the corn seeds are directly soaked with it for 24 hours, which is too long.

[0028] Based on the above reasons, the present invention provides a treatment method for promoting aseptic germination of corn seeds, comprising:

[0029] (1) Take corn seeds and sterilize them.

[0030] (2) Seed soaking: Add the sterilized corn seeds into sterile water and soak them at room temperature for 6 to 8 hours;

[0031] (3) Dark culture: First, sterilize the corn seed germination device, and evenly place the soaked corn seeds in the corn seed germination device; then add sterile water to the corn seed germination device to allow the corn seed germination device to absorb and retain water that can cause the corn seeds to germinate; finally, place the corn seed germination device in an environment of 24°C to 26°C for dark culture (without light) until the corn seeds germinate; see the actual picture of corn seed germination for details. Figure 1 The corn seed germination devices are neatly placed on the racks, and different degrees of germination have occurred.

[0032] The method of seed disinfection is: soak and disinfect in a sodium hypochlorite solution with a mass fraction of 0.9% to 1.1% for 15 minutes, and then rinse with a sterile osmotic regulator.

[0033] The present invention uses sterile water to soak seeds at room temperature for 6h to 8h, and cooperates with technical features such as "immersing and disinfecting with a mass fraction of 1% sodium hypochlorite solution for 15min to 20min" to perform disinfection and osmotic adjustment, which not only achieves a disinfection effect and avoids pollution, but also achieves an osmotic adjustment effect to accelerate corn seed germination and avoid conflicts with the control of genes and metabolic pathways. Therefore, in the present invention, potassium sorbate is used to promote the germination of corn seeds, overcoming the defects of the prior art. At the same time, the present invention also provides a 3g / L to 12g / L sterile potassium sorbate solution or a 0.05g / L to 0.1g / L sterile lead nitrate solution, or a combination of either of the two and sterile water, as an application of a sterile osmotic regulator, which can also avoid conflicts with the control of genes and metabolic pathways and promote corn seed germination.

[0034] In order to reduce pollution during the seed soaking process, the prior art usually adopts low-temperature seed soaking at 10°C. Low-temperature seed soaking consumes energy of refrigeration equipment and is not energy-saving. The present invention soaks seeds at room temperature, reducing the energy consumption of refrigeration equipment.

[0035] The main instruments used in the experiments of the embodiments and comparative examples of the present invention are an artificial climate box, a corn seed germination bag, a placing rack, and tweezers.

[0036] In the description of the present invention, unless otherwise specified, all reagents used are commercially available, and all methods used are conventional techniques in the art. The corn seed germination bag used in the following examples and experiments includes a transparent bag and a paper core located inside the transparent bag. The size of the corn seed germination bag is 14 cm long and 12.5 cm wide, that is, the size of the transparent bag is 14 cm long and 12.5 cm wide; the transparent bag is a plastic bag, the paper core is absorbent paper, and the absorbent paper is filter paper.

[0037] The room temperature commonly used in experimental subjects is 20° C. to 25° C. Therefore, the present invention has conducted experiments in this temperature range. The pH is adjusted with hydrochloric acid or sodium hydroxide.

[0038] Example 1

[0039] A method for promoting aseptic germination of corn seeds comprises the following steps:

[0040] (1) Seed selection: Select corn seeds with full, uniform, and not white grains for seed disinfection.

[0041] (2) Seed soaking: Soak the sterilized corn seeds in sterile water at room temperature (20°C) for 6 hours.

[0042] (3) Dark culture: First, sterilize the corn seed germination bag and the tweezers used for inoculation (sterilize with 75% alcohol by volume); evenly place 30 soaked corn seeds in the paper core of the corn seed germination bag; then add 40 mL of sterile water to fully soak the paper core and retain moisture that can make the corn seeds germinate. Finally, place the germination bag in an artificial climate box at 20°C for dark culture until the corn seeds germinate.

[0043] The disinfection methods in (1) are: soaking and disinfecting in a 1% sodium hypochlorite solution for 15 minutes, then rinsing the corn seeds in a sterile osmotic regulator solution for 1 minute, then draining the water, then rinsing the corn seeds in a sterile osmotic regulator solution for 1 minute, then draining the water, then rinsing the corn seeds in a sterile osmotic regulator solution for 1 minute, then draining the water. The sterile osmotic regulator solution is a 12g / L sterile potassium sorbate solution.

[0044] Example 2

[0045] A method for promoting aseptic germination of corn seeds comprises the following steps:

[0046] (1) Seed selection: Select corn seeds with full, uniform, and not white grains for seed disinfection.

[0047] (2) Seed soaking: Soak the sterilized corn seeds in sterile water at room temperature of 25°C for 6 hours.

[0048] (3) Dark culture: First, sterilize the paper core in the germination bag and the tweezers used for inoculation (sterilize with 75% alcohol by volume); evenly place 30 soaked corn seeds in the paper core in the germination bag; then add 60 mL of sterile water to fully soak the paper core and retain moisture that can make the corn seeds germinate. Finally, place the germination bag in an artificial climate box at 25°C for dark culture until the corn seeds germinate.

[0049] The seed disinfection method is: soak and disinfect in a 1% sodium hypochlorite solution for 15 minutes, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water. The sterile osmotic regulator solution is a 3g / L sterile potassium sorbate solution with a pH of 6.0, and the sterile osmotic regulator is passed through 3 times.

[0050] Example 3

[0051] A method for promoting aseptic germination of corn seeds comprises the following steps:

[0052] (1) Seed selection: Select corn seeds with full, uniform, and not white grains for seed disinfection.

[0053] (2) Seed soaking: Soak the sterilized corn seeds in sterile water at room temperature of 20°C for 8 hours.

[0054] (3) Dark culture: First, sterilize the paper core in the germination bag and the tweezers used for inoculation (disinfect with 75% alcohol by volume); evenly place 30 soaked corn seeds in the paper core in the germination bag, which is filter paper; then add 50 mL of sterile water to fully soak the paper core and retain moisture that can make the corn seeds germinate. Finally, place the germination bag in an artificial climate box at 20°C for dark culture until the corn seeds germinate.

[0055] The seed disinfection method is: soak and disinfect in a 1% sodium hypochlorite solution for 15 minutes, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water. The sterile osmotic regulator solution is a 6g / L sterile potassium sorbate solution with a pH of 4.0.

[0056] Example 4

[0057] A method for promoting aseptic germination of corn seeds comprises the following steps:

[0058] (1) Seed selection: Select corn seeds with full, uniform, and not white grains for seed disinfection.

[0059] (2) Seed soaking: Soak the sterilized corn seeds in sterile water at room temperature of 20°C for 6 hours.

[0060] (3) Dark culture: First, sterilize the paper core in the germination bag and the tweezers used for inoculation (disinfect with 75% alcohol by volume); evenly place 30 soaked corn seeds in the paper core in the germination bag, which is filter paper; then add 40 mL of sterile water to fully soak the paper core and retain moisture that can make the corn seeds germinate. Finally, place the germination bag in an artificial climate box at 20°C for dark culture until the corn seeds germinate.

[0061] The seed disinfection method is: soak and disinfect in a 1% sodium hypochlorite solution for 15 minutes, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water. The sterile osmotic regulator solution is a 0.1g / L sterile lead nitrate solution.

[0062] Example 5

[0063] A method for promoting aseptic germination of corn seeds comprises the following steps:

[0064] (1) Seed selection: Select corn seeds with full, uniform, and not white grains for seed disinfection.

[0065] (2) Seed soaking: Soak the sterilized corn seeds in sterile water at room temperature of 20°C for 6 hours.

[0066] (3) Dark culture: First, sterilize the paper core in the germination bag and the tweezers used for inoculation (disinfect with 75% alcohol by volume); evenly place 30 soaked corn seeds in the paper core in the germination bag, which is filter paper; then add 40 mL of sterile water to fully soak the paper core and retain moisture that can make the corn seeds germinate. Finally, place the germination bag in an artificial climate box at 20°C for dark culture until the corn seeds germinate.

[0067] The seed disinfection method is: soak and disinfect in a 1% sodium hypochlorite solution for 15 minutes, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water, then rinse the corn seeds in a sterile osmotic regulator solution for 1 minute, then drain the water. The sterile osmotic regulator solution is a 0.05g / L sterile lead nitrate solution.

[0068] Example 6

[0069] A method for promoting aseptic germination of corn seeds comprises the following steps:

[0070] (1) Seed selection: Select corn seeds with full, uniform, and not white grains for seed disinfection.

[0071] (2) Seed soaking: Soak the sterilized corn seeds in sterile water at room temperature of 20°C for 6 hours.

[0072] (3) Dark culture: First, sterilize the paper core in the germination bag and the tweezers used for inoculation (disinfect with 75% alcohol by volume); evenly place 30 soaked corn seeds in the paper core in the germination bag, which is filter paper; then add 40 mL of sterile water to fully soak the paper core and retain moisture that can make the corn seeds germinate. Finally, place the germination bag in an artificial climate box at 20°C for dark culture until the corn seeds germinate.

[0073] The method for seed disinfection is: the corn seeds after being soaked and disinfected in a 1% sodium hypochlorite solution for 15 minutes are placed in a 3g / L sterile potassium sorbate solution at 48°C and pH 6.0 for 1 minute, then drained, then placed in a 3g / L sterile potassium sorbate solution at 48°C and pH 6.0 for 1 minute, then drained, then placed in sterile water at 48°C and pH 6.0 for 1 minute, and drained.

[0074] Example 7

[0075] A method for promoting aseptic germination of corn seeds comprises the following steps:

[0076] (1) Seed selection: Select corn seeds with full, uniform, and not white grains for seed disinfection.

[0077] (2) Seed soaking: Soak the sterilized corn seeds in sterile water at room temperature of 20°C for 6 hours.

[0078] (3) Dark culture: First, sterilize the paper core in the germination bag and the tweezers used for inoculation (disinfect with 75% alcohol by volume); evenly place 30 soaked corn seeds in the paper core in the germination bag, which is filter paper; then add 40 mL of sterile water to fully soak the paper core and retain moisture that can make the corn seeds germinate. Finally, place the germination bag in an artificial climate box at 20°C for dark culture until the corn seeds germinate.

[0079] The method for seed disinfection is: the corn seeds after being soaked and disinfected in a 1% sodium hypochlorite solution for 15 minutes are placed in a 6g / L sterile potassium sorbate solution at 48°C and pH 6.0 for 1 minute, then drained, then placed in a 6g / L sterile potassium sorbate solution at 48°C and pH 6.0 for 1 minute, then drained, then placed in sterile water at 48°C and pH 6.0 for 1 minute, and drained.

[0080] Example 8

[0081] A method for promoting aseptic germination of corn seeds comprises the following steps:

[0082] (1) Seed selection: Select corn seeds with full, uniform, and not white grains for seed disinfection.

[0083] (2) Seed soaking: Soak the sterilized corn seeds in sterile water at room temperature of 20°C for 6 hours.

[0084] (3) Dark culture: First, sterilize the paper core in the germination bag and the tweezers used for inoculation (disinfect with 75% alcohol by volume); evenly place 30 soaked corn seeds in the paper core in the germination bag, which is filter paper; then add 40 mL of sterile water to fully soak the paper core and retain moisture that can make the corn seeds germinate. Finally, place the germination bag in an artificial climate box at 20°C for dark culture until the corn seeds germinate.

[0085] The method for seed disinfection is: the corn seeds are soaked and disinfected in a 1% sodium hypochlorite solution for 15 minutes, then rinsed in a 12g / L sterile potassium sorbate solution at 48°C and pH 6.0 for 1 minute, then drained, then rinsed in a 12g / L sterile potassium sorbate solution at 48°C and pH 6.0 for 1 minute, then drained, then rinsed in sterile water at 48°C and pH 6.0 for 1 minute, and drained.

[0086] Example 9

[0087] A method for promoting aseptic germination of corn seeds comprises the following steps:

[0088] (1) Seed selection: Select corn seeds with full, uniform, and not white grains for seed disinfection.

[0089] (2) Seed soaking: Soak the sterilized corn seeds in sterile water at room temperature of 20°C for 6 hours.

[0090] (3) Dark culture: First, sterilize the paper core in the germination bag and the tweezers used for inoculation (disinfect with 75% alcohol by volume); evenly place 30 soaked corn seeds in the paper core in the germination bag, which is filter paper; then add 40 mL of sterile water to fully soak the paper core and retain moisture that can make the corn seeds germinate. Finally, place the germination bag in an artificial climate box at 20°C for dark culture until the corn seeds germinate.

[0091] The method for seed disinfection is: the corn seeds are soaked and disinfected in a 1% sodium hypochlorite solution for 15 minutes, then rinsed in a 0.05 g / L sterile lead nitrate solution at 45°C and pH 4.0 for 1 minute, then drained, then rinsed in a 0.05 g / L sterile lead nitrate solution at 45°C and pH 4.0 for 1 minute, then drained, then rinsed in sterile water at 45°C and pH 4.0 for 1 minute, and drained.

[0092] Example 10

[0093] A method for promoting aseptic germination of corn seeds comprises the following steps:

[0094] (1) Seed selection: Select corn seeds with full, uniform, and not white grains for seed disinfection.

[0095] (2) Seed soaking: Soak the sterilized corn seeds in sterile water at room temperature of 20°C for 6 hours.

[0096] (3) Dark culture: First, sterilize the paper core in the germination bag and the tweezers used for inoculation (disinfect with 75% alcohol by volume); evenly place 30 soaked corn seeds in the paper core in the germination bag, which is filter paper; then add 40 mL of sterile water to fully soak the paper core and retain moisture that can make the corn seeds germinate. Finally, place the germination bag in an artificial climate box at 20°C for dark culture until the corn seeds germinate.

[0097] The method for seed disinfection is: the corn seeds after being soaked and disinfected in a 1% sodium hypochlorite solution for 15 minutes are placed in a 0.1g / L sterile lead nitrate solution at 45°C and pH4.0 for 1 minute, then drained, then placed in a 0.1g / L sterile lead nitrate solution at 45°C and pH4.0 for 1 minute, then drained, then placed in sterile water at 45°C and pH4.0 for 1 minute, and drained.

[0098] In order to further demonstrate the research effect of the present invention, we also conducted the following experiments.

[0099] 1. Effects of different disinfectants on corn seed germination

[0100] Seed disinfection method: Soak corn seeds in disinfectant for 15 minutes, without rinsing with sterile osmotic regulator.

[0101] Other operation steps refer to Example 1.

[0102] After 7 days, the germination potential, germination rate and mildew rate were counted.

[0103] Table 1 Effects of different disinfectants on corn seed germination

[0104] Disinfectant Type Germination potential (%) Germination rate (%) Mildew rate (%) 1% sodium hypochlorite solution 88.45±0.12 90.24±0.06 9.30±0.05 75% ethanol solution 87.29±0.09 89.71±0.10 9.56±0.05 12g / L Potassium Sorbate Solution 60.18±0.13 63.00±0.05 4.45±0.08 0.1g / L sterile lead nitrate solution 45.17±0.08 46.28±0.10 20.07±0.11 Sterile water 71.93±0.09 73.11±0.09 33.05±0.08

[0105] The results in Table 1 show that different disinfectants have different effects on the germination and mildew rate of corn seeds. The germination rate of corn seeds treated with 1% sodium hypochlorite solution is the highest, while the mildew rate of corn seeds treated with 12g / L potassium sorbate solution is the lowest.

[0106] 2. Effects of different disinfectant concentrations on corn seed germination

[0107] Based on the experimental results in Table 1, sodium hypochlorite and potassium sorbate were used as disinfectants, and different disinfectant concentrations were set.

[0108] Seed disinfection method: Soak corn seeds in disinfectant for 15 minutes, without rinsing with sterile osmotic regulator.

[0109] The other operation steps were as in Example 1. After 7 days, the germination potential, germination rate and mildew rate were counted to evaluate the germination effect of corn seeds.

[0110] Table 2 Effects of different disinfectant concentrations on corn seed germination

[0111]

[0112]

[0113] The results in Table 2 show that the treatments in the two concentration ranges of 0.9% to 1.1% sodium hypochlorite solution (germination rate>89%, mildew rate<10%) and 3g / L to 12g / L potassium sorbate solution (germination rate>63%, mildew rate<5%) can achieve a higher germination rate and a lower mildew rate. Among them, the comprehensive effect of the treatment with 0.9% to 1.1% sodium hypochlorite solution is better than that with 3g / L to 12g / L potassium sorbate solution.

[0114] Based on the experimental results in Table 1 and Table 2, the embodiment of the present invention selects sodium hypochlorite solution and potassium sorbate solution for consideration of antibacterial effect, germination rate and germination potential, and changes the use method of potassium sorbate solution.

[0115] 3. Effects of different disinfection methods on corn seed germination

[0116] Seed disinfection method is configured as shown in Table 3, and other operations are the same as in Example 1. After 7 days, germination potential, germination rate and mildew rate are counted.

[0117] Table 3 Effects of different disinfection methods on corn seed germination

[0118]

[0119] The results in Table 3 show that the seed disinfection methods of each embodiment have a lower mildew rate, higher germination rate and germination potential than other seed disinfection methods due to the use of multiple sterile osmotic regulator rinsing methods and the limitation of the temperature and pH of the sterile osmotic regulator solution. Among them, after the combination of sodium hypochlorite solution and potassium sorbate solution, or sodium hypochlorite solution and lead nitrate solution, the germination effect of corn seeds is better (germination potential>96%, germination rate>97%), and the mildew rate is lower (mildew rate<3%).

[0120] 4. Effects of different disinfection times on corn seed germination

[0121] Seed disinfection method is configured as shown in Table 4, and other operations are the same as in Example 1. After 7 days, germination potential, germination rate and mildew rate are counted.

[0122] Table 4 Effects of different disinfection times on corn seed germination

[0123]

[0124]

[0125] The results in Table 4 show that if the seed disinfection time is too short, the mildew rate will increase significantly, and if the disinfection time is too long, the germination rate and germination potential will decrease significantly, which will have a great impact on the corn seeds. Therefore, the disinfection time should be appropriate.

[0126] It should be noted that when the present invention involves a numerical range, it should be understood that the two endpoints of each numerical range and any value between the two endpoints can be selected. Since the steps and methods used are the same as those in the embodiments, in order to avoid redundancy, the present invention describes a preferred embodiment. Although the preferred embodiments of the present invention have been described, once those skilled in the art know the basic inventive concept, they can make other changes and modifications to these embodiments, and these changes and modifications all fall within the scope of the present invention.

[0127] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. If these modifications and variations of the present invention fall within the scope of the equivalent technology of the present invention, the present invention is also intended to include these modifications and variations.

Claims

1. A method for promoting aseptic germination of corn seeds, characterized in that: include: Take corn seeds and sterilize them; Add the sterilized corn seeds into sterile water and soak them at room temperature for 6 to 8 hours; The corn seed germination device is sterilized, and the soaked corn seeds are placed in the corn seed germination device; sterile water is added into the corn seed germination device so that the corn seed germination device absorbs and retains water that can make the corn seeds germinate; finally, the corn seed germination device is placed in an environment of 24° C. to 26° C. for dark culture until the corn seeds germinate; The method of seed disinfection is as follows: soak corn seeds in a sodium hypochlorite solution with a mass fraction of 0.9% to 1.1%, disinfect for 15 minutes to 20 minutes, and then rinse with a sterile osmotic regulator; The sterile osmotic regulator is a 3g / L to 12g / L sterile potassium sorbate solution or a 0.05g / L to 0.1g / L sterile lead nitrate solution, or a combination of either of the two and sterile water.

2. The method for promoting aseptic germination of corn seeds according to claim 1, characterized in that: The temperature of the sterile osmotic regulator is 45℃~48℃.

3. The method for promoting aseptic germination of corn seeds according to claim 1, characterized in that: The pH of the sterile osmotic regulator is 4.0 to 6.

0.

4. The method for promoting aseptic germination of corn seeds according to claim 1, characterized in that: Rinse with sterile osmotic regulator 3 to 5 times, and the sterile osmotic regulator used for the last time is sterile water.

5. The method for promoting aseptic germination of corn seeds according to claim 1, characterized in that: The method of rinsing with a sterile osmotic regulator is as follows: the corn seeds that have been sterilized are soaked in a 0.9% to 1.1% sodium hypochlorite solution by mass, and then rinsed in the sterile osmotic regulator solution for 1 minute to 2 minutes, and then the water is drained.

6. The method for promoting aseptic germination of corn seeds according to claim 1, characterized in that: Room temperature refers to 20℃~25℃.

7. The method for promoting aseptic germination of corn seeds according to claim 1, characterized in that: The corn seed germination device is a corn seed germination bag, which includes a transparent bag and a paper core located inside the transparent bag. The soaked corn seeds are placed in the paper core, and then sterile water is added into the paper core to allow the paper core to fully absorb and retain moisture that can make the corn seeds germinate.

8. The method for promoting aseptic germination of corn seeds according to claim 7, characterized in that: The size of the corn seed germination bag is 14cm to 18cm in length and 12.5cm to 14cm in width.

9. The method for promoting aseptic germination of corn seeds according to claim 8, characterized in that: Place 10 to 30 soaked corn seeds in each corn seed germination bag.

10. The method for promoting aseptic germination of corn seeds according to claim 8, characterized in that: The amount of water required to fully soak the paper core and retain enough water for the corn seeds to germinate is 40mL to 60mL.

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