Composition for inhibiting pathogenic bacteria on surfaces of oral attachments and preparation method thereof

Abstract

The invention discloses a composition for inhibiting pathogenic bacteria on the surfaces of oral attachments and a preparation method thereof.The composition for inhibiting the pathogenic bacteria on the surfaces of the oral attachments comprises a composition 1 and a composition 2 which are stored separately, and the composition 1 is prepared from, by mass, 1-2% of punicalagin, 9-11% of malic acid, 2-3% of cellobiase and 2-3% of celloglucanase; the cleaning agent is prepared from, by weight, 3-5% of sodium lauryl sulfate, 15-20% of ethylene diamine tetraacetic acid, 1-3% of glycerin and the balance deionized water. And the composition 2 comprises the following components in percentage by mass: 0.1-0.5% of myrtenol, 1-3% of glycerol, 3-5% of ethanol, 4-7% of propylene glycol, 1-2% of borax and the balance of deionized water. The composition can specifically inhibit three oral cavity germs, namely porphyromonas, streptococcus and Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus Actinobacillus

Description

Technical Field

[0001] The invention relates to the technical field of oral medicine, and in particular to a composition for inhibiting pathogenic bacteria on the surface of oral attachments and a preparation method thereof. Background Art

[0002] During oral treatment, various treatment accessories are often worn into the oral cavity, such as the more common orthodontic accessory brackets, artificial crowns, inlays and veneers for oral restoration. There are many kinds of bacteria in daily life, and it is impossible to eliminate all bacteria in the living environment. The three oral specific pathogens that are more harmful to the oral cavity include Porphyromonas, Streptococcus and Aggregatibacter actinomycetemcomitans. They are specific bacteria that cause a variety of oral diseases and can easily lead to loosening and falling of teeth. Therefore, the treatment of accessories worn in the mouth must first inhibit these three specific bacteria.

[0003] In the living environment, various bacteria and dirt will contaminate the accessories that need to be worn in the mouth; another situation is that the surface of the accessories in the mouth has a collagen matrix. Although it is not a direct component of the bacteria, it will help the bacteria form a stable external scaffold structure and assist in the formation of a three-dimensional community structure, and it is not easy to fall off from the surface of the accessories in the mouth.

[0004] In view of the above situation, the dental community uses a variety of methods to remove it, such as 75% alcohol disinfection, mouthwash soaking, potassium permanganate liquid soaking, or hydrogen peroxide washing, but many methods have inherent defects. For example, 75% alcohol soaking often accelerates the aging of the attachments, mouthwash disinfection efficiency is low, and there are often problems such as pigment residue after potassium permanganate treatment. More importantly, the ordinary antibacterial and bactericidal effect mainly refers to killing the bacteria itself, but it cannot cut off the external nutritional structure (hyphae) of the bacteria itself, and it cannot disintegrate the collagen scaffold on the surface of the dirt.

[0005] In order to solve the above technical problems, it is necessary to develop a composition for inhibiting pathogenic bacteria on the surface of oral attachments. Summary of the invention

[0006] The purpose of the present invention is to provide a composition for inhibiting pathogenic bacteria on the surface of oral attachments, which can specifically inhibit three oral pathogens, namely Porphyromonas, Streptococcus and Aggregatibacter actinomycetemcomitans, while being able to destroy the collagen scaffold on the surface of oral attachments and specifically disintegrate the nutrient transport channel (hyphae) outside the bacterial body.

[0007] In order to achieve the above object, the present invention adopts the following technical solution:

[0008] In a first aspect of the present invention, a composition for inhibiting pathogenic bacteria on the surface of oral attachments is provided. The composition for inhibiting pathogenic bacteria on the surface of oral attachments comprises composition 1 and composition 2 stored separately.

[0009] The composition 1 comprises, by mass fraction, 1-2% punicalagin, 9-11% malic acid, 2-3% cellobiase, 2-3% celloglucanase, 3-5% sodium lauryl sulfate, 15-20% disodium ethylenediaminetetraacetic acid, 1-3% glycerol, and the remainder is deionized water, and the total amount is 100%;

[0010] The composition 2 comprises, by mass fraction, 0.1-0.5% myrtenol, 1-3% glycerol, 3-5% ethanol, 4-7% propylene glycol, 1-2% borax, and the remainder is deionized water, and the total amount is 100%.

[0011] In a second aspect of the present invention, a method for preparing a composition for inhibiting pathogenic bacteria on the surface of oral attachments is provided, the method comprising:

[0012] Firstly, the components of the composition 1 are weighed, and dissolving disodium ethylenediaminetetraacetate into deionized water; then sodium lauryl sulfate is added to dissolve, and then punicalagin, malic acid, cellobiase and celloglucanase are added to dissolve, so as to obtain the composition 1;

[0013] Firstly, the components of the composition 2 are weighed, glycerin is dissolved in deionized water, and then ethanol, propylene glycol, borax and myrtenol are added in sequence to dissolve, so as to obtain the composition 2.

[0014] In the above technical solution, the ingredients are added in the above order to prevent interaction between them and facilitate dissolution.

[0015] One or more technical solutions in the embodiments of the present invention have at least the following technical effects or advantages:

[0016] 1. The present invention provides a composition for inhibiting pathogenic bacteria on the surface of oral appendages. It has been found through experiments that the combined use of 1-2% punicalagin and 0.1-0.5% myrtenol is 17 times more effective than the use of punicalagin or myrtenol alone, producing a synergistic effect that can specifically inhibit three specific oral bacteria.

[0017] 2. The destructive effect of 0.1-0.5% myrtenol in a composition for inhibiting pathogenic bacteria on the surface of oral adnexa provided by the present invention on the collagen scaffold on the surface of oral adnexa is not found in other documents and inventions.

[0018] 3. The oral sterilization in the prior art kills the bacteria themselves and reduces the amount of bacteria, but the nutrient transport (hyphae) outside the bacteria is not disrupted. The composition for inhibiting pathogenic bacteria on the surface of oral appendages provided by the present invention can also specifically disrupt the nutrient transport channel (hyphae) outside the bacteria body. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0020] Figure 1 The graph shows the inhibitory effect of compositions at different gradient concentrations on bacteria.

[0021] Figure 2 This is a graph showing the obvious synergistic effect of punicalagin and myrtenol on bacterial inhibition.

[0022] Figure 3 This is a graph showing the significant degradation effect of myrtle alcohol on collagen scaffolds.

[0023] Figure 4 The thickness of the whole bacterial plaque is shown by atomic force microscopy. The thickness of the whole bacterial plaque before treatment (left picture) and the thickness of the whole bacterial plaque after treatment of the present invention (right picture) are compared.

[0024] Figure 5 Topographic maps reconstructed from surface morphology microscopy data showing bacterial morphology before and after treatment.

[0025] Figure 6 It is an inhibitory effect on the nutrient transport pathway (hyphae) outside the bacteria itself.

[0026] Figure 7 The PCR results before and after treatment are used to detect the inhibitory effect on three oral specific pathogens; PCR detection of the three major oral pathogens in the overall colony, including Porphyromonas, Streptococcus and Aggregatibacter actinomycetemcomitans. Comparison of the four major pathogens before treatment (left picture) and after treatment by the present invention (right picture).

[0027] Figure 8 The figure is a comparison diagram of the changes of harmful products of bacterial plaque detected by Western blot; the total products LPS, IL-1β and TNF-α of the whole bacterial colony before treatment and the total products LPS, IL-1β and TNF-α of the whole bacterial colony after treatment of the present invention.

[0028] Fig. 9 To investigate the morphology and content of collagen in bacterial scaffolds. DETAILED DESCRIPTION

[0029] The present invention will be described in detail below in conjunction with specific implementations and examples, and the advantages and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific implementations and examples are used to illustrate the present invention, rather than to limit the present invention.

[0030] Throughout the specification, unless otherwise specifically stated, the terms used herein should be understood as meanings commonly used in the art. Therefore, unless otherwise defined, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art to which the present invention belongs. In the event of a conflict, the present specification takes precedence.

[0031] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or obtained by existing methods.

[0032] The technical solution of the embodiment of the present application is to solve the above technical problems, and the overall idea is as follows:

[0033] According to a typical embodiment of the present invention, a composition for inhibiting pathogenic bacteria on the surface of oral attachments is provided, wherein the composition for inhibiting pathogenic bacteria on the surface of oral attachments comprises composition 1 and composition 2 stored separately,

[0034] The composition 1 comprises, by mass fraction, 1-2% punicalagin, 9-11% malic acid, 2-3% cellobiase, 2-3% celloglucanase, 3-5% sodium lauryl sulfate, 15-20% disodium ethylenediaminetetraacetic acid, 1-3% glycerol, and the balance is deionized water;

[0035] The composition 2 comprises, by mass fraction, 0.1-0.5% myrtenol, 1-3% glycerol, 3-5% ethanol, 4-7% propylene glycol, 1-2% borax, and the balance is deionized water.

[0036] In the above technical solution,

[0037] The combination of 1-2% punicalagin and 0.1-0.5% myrtenol can specifically inhibit three oral pathogens, namely Porphyromonas, Streptococcus and Aggregatibacter actinomycetemcomitans; in addition, 0.1-0.5% myrtenol has a destructive effect on the collagen scaffold on the surface of oral attachments.

[0038] The function of malic acid: inhibit the formation of soft plaque on the surface of oral appendages.

[0039] Functions of cellobiase and celloglucanase: Degradation of food residues in waste

[0040] According to another typical embodiment of the present invention, a method for using the composition for inhibiting pathogenic bacteria on the surface of oral attachments is provided as follows:

[0041] Step 1: Rinse the orthodontic or restorative accessories, dirt, blood, saliva, soft scale, etc. with deionized water.

[0042] Step 2: Treat the accessories in composition 1 and rinse with deionized water.

[0043] Step 3: Place the attachment in the treatment composition 2 and rinse it with deionized water using a dental three-way air gun.

[0044] Step 4: Place the treated accessories into an ultrasonic cleaner for a final shock rinse.

[0045] Preferably, the processing time of step 2 is 3-5 minutes.

[0046] Preferably, the processing time of step 3 is 5-10 minutes.

[0047] Preferably, the flushing time of the three-purpose air gun in step 3 is 10-30 seconds.

[0048] Preferably, the flushing liquid of the three-purpose air gun in step 3 is deionized water.

[0049] Preferably, the ultrasonic cleaning time in step 4 is 2-5 minutes.

[0050] The composition for inhibiting pathogenic bacteria on the surface of oral appendages of the present application will be described in detail below in combination with embodiments, comparative examples and experimental data.

[0051] Example 1

[0052] 1. This embodiment provides a composition for inhibiting pathogenic bacteria on the surface of oral attachments. The composition for inhibiting pathogenic bacteria on the surface of oral attachments comprises composition 1 and composition 2 stored separately.

[0053] The composition 1 comprises, by mass fraction, 1.5% punicalagin, 10% malic acid, 2.5% cellobiase, 2.5% celloglucanase, 4% sodium lauryl sulfate, 17.5% disodium ethylenediaminetetraacetic acid, 2% glycerol, and the balance is deionized water.

[0054] The composition 2 comprises, by mass fraction, 0.25% myrtenol, 2% glycerol, 4% ethanol, 5% propylene glycol, 1.5% borax, and the balance is deionized water.

[0055] 2. Usage:

[0056] (1) With the patient's consent, remove the fallen tooth inlay.

[0057] (2) Rinse the inlay of dirt, blood, saliva, soft scale, etc. with deionized water.

[0058] (3) Place the attachment in the composition 1 for 5 minutes and rinse with deionized water.

[0059] (4) The attachments were placed in the composition 2 for treatment for 10 minutes.

[0060] (5) Rinse with deionized water and use a dental three-in-one air gun for 30 seconds.

[0061] (6) Place the treated accessories in an ultrasonic cleaning machine for a final oscillation rinse. The cleaning time is 5 minutes.

[0062] Example 2

[0063] This embodiment provides a composition for inhibiting pathogenic bacteria on the surface of oral appendages, wherein the composition for inhibiting pathogenic bacteria on the surface of oral appendages comprises composition 1 and composition 2 stored separately;

[0064] The composition 1 comprises, by mass fraction, 1% punicalagin, 9% malic acid, 2% cellobiase, 2% celloglucanase, 3% sodium lauryl sulfate, 15% disodium ethylenediaminetetraacetic acid, 1% glycerol, and the balance is deionized water;

[0065] The composition 2 comprises, by mass fraction, 0.1% myrtenol, 1% glycerol, 3% ethanol, 4% propylene glycol, 1% borax, and the balance is deionized water.

[0066] Example 3

[0067] This embodiment provides a composition for inhibiting pathogenic bacteria on the surface of oral appendages, wherein the composition for inhibiting pathogenic bacteria on the surface of oral appendages comprises composition 1 and composition 2 stored separately;

[0068] The composition 1 comprises, by mass fraction, 2% punicalagin, 11% malic acid, 3% cellobiase, 3% celloglucanase, 5% sodium lauryl sulfate, 20% disodium ethylenediaminetetraacetic acid, 3% glycerol, and the balance is deionized water;

[0069] The composition 2 comprises, by mass fraction, 0.5% myrtenol, 3% glycerol, 5% ethanol, 7% propylene glycol, 2% borax, and the balance is deionized water.

[0070] Comparative Example 1

[0071] In this comparative example, the simple composition 1: punicalagin. It is configured as follows: 1.5% punicalagin, 10% malic acid, 2.5% cellobiase, 2.5% celloglucanase, 4% sodium lauryl sulfate, 17.5% disodium ethylenediaminetetraacetic acid, 2% glycerol, and the balance is deionized water.

[0072] Comparative Example 2

[0073] In this comparative example, the simple composition 2: myrtenol is prepared by 0.25% myrtenol, 2% glycerol, 4% ethanol, 5% propylene glycol, 1.5% borax, and the balance is deionized water.

[0074] Comparative Example 3

[0075] This comparative example does not contain myrtenol, and the rest is the same as Example 1. The specific configuration is: 2% glycerol, 4% ethanol, 5% propylene glycol, 1.5% borax, and the balance is deionized water.

[0076] Example 4

[0077] The composition 1 is composed of the following compounds: 2% punicalagin, 10% malic acid, 3% cellobiase, 3% celloglucanase, 5% sodium lauryl sulfate, 200% disodium ethylenediaminetetraacetic acid, 3% glycerol, and the balance is deionized water.

[0078] Composition 2 is composed of the following compounds: 0.5% myrtle alcohol, 3% glycerol, 5% ethanol, 7% propylene glycol, 2% borax, and the balance is deionized water.

[0079] Example 5-Example 10

[0080] The components of Example 5 to Example 7 are arranged as shown in Table 1, and the components of Example 8 to Example 10 are arranged as shown in Table 2.

[0081] Comparative Example 4-Comparative Example 15

[0082] The components of Comparative Examples 4 to 8 are set as shown in Table 1, and the components of Comparative Examples 9 to 15 are set as shown in Table 2.

[0083] Experimental Example 1

[0084] 1. Comparison of grouping of punicalagin gradient concentration (Examples 5-7 and Comparative Examples 4-8): 8 gradient content groups were set, containing 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, 5.0%, 10.0% of punicalagin respectively, and the remaining ingredients were: 10% malic acid, 2.5% cellobiase, 2.5% celloglucanase, 4% sodium lauryl sulfate, 17.5% disodium ethylenediaminetetraacetic acid, 2% glycerol, and the balance was deionized water. Divided into 8 groups, they were applied to the surface of the brackets respectively, and then the content of three specific bacteria was measured by realtime PCR. Each group is shown in Table 1.

[0085] Table 1

[0086]

[0087]

[0088] From the results in Table 1, it can be seen that when the specific content of punicalagin is between 1.0-2.0%, its efficiency in inhibiting specific bacteria is the highest.

[0089] 2. Set 10 gradient content groups (Examples 5-7 and Comparative Examples 4-8), respectively containing myrtle alcohol content of 0.01%, 0.05%, 0.1%, 0.25%, 0.5%, 0.6%, 1.0%, 2.0%, 5.0%, 10.0%, and the remaining ingredients are: 2% glycerol, 4% ethanol, 5% propylene glycol, 1.5% borax, and the balance is deionized water. Divide into 10 groups, apply them to the surface of the bracket respectively, and then measure the content of three specific bacteria by realtime PCR method. Each group is shown in Table 2.

[0090] Table 2

[0091]

[0092]

[0093] It can be seen from Table 2 that when the specific content of myrtle alcohol is between 0.1% and 0.5%, its efficiency in inhibiting specific bacteria is the highest.

[0094] Experimental Example 2

[0095] The compositions of Example 1, Comparative Example 1 and Comparative Example 2 were applied to the surface of the brackets respectively, and then the contents of three specific bacteria were measured. The results are shown in Table 3.

[0096] Table 3

[0097]

[0098] From Table 3, we can see that:

[0099] The specific antibacterial (Porphyromonas, Streptococcus and Aggregatibacter actinomycetemcomitans) effects of the group using Composition 1 and Composition 2 together were determined by RealtimePCR method, and the difference was nearly 10.53-17.74 times, reaching a statistically significant difference, indicating that the specific concentration and specific combination effect of the present invention are the outstanding features of the present invention.

[0100] Experimental Example 3: Detection of active ingredients in anti-collagen scaffolds

[0101] Example 1 and Comparative Example 3 were applied to the surface of the bracket respectively, and then the collagen content before and after the treatment was measured.

[0102] The results are as follows Figure 3As shown, for the group without myrtenol, there was no significant difference in collagen content before and after treatment. However, for the group containing myrtenol in the composition of the present invention, the collagen content after treatment was significantly lower than that before treatment. This indicates that myrtenol has an inhibitory and destructive effect on collagen in the oral appendages.

[0103] Experimental Example 4: Specific disruption of nutrient transport pathways outside the bacterial body

[0104] 1. The composition of Example 4 was used to perform relevant preoperative tests on oral brackets. The test contents and methods are as follows:

[0105] 1. Atomic force microscopy shows plaque thickness: Use a microscope to observe the bracket surface, record the thickness of the soft scale in the vertical distance direction of the side, and complete 3D reconstruction. Organize it into a data graph.

[0106] 2. Surface morphology microscope shows bacterial colony shape: mainly includes two aspects, bacterial colony morphology and colony maturity. Detect the growth morphology and surface layout of the bacterial colony, and calculate the difference in bacterial colony morphology through the topographic map calculation formula of the atomic force microscope to comprehensively evaluate the maturity of its bacterial colony morphology.

[0107] 3. PCR result measurement: PCR mainly detects the presence or absence of three oral-specific pathogens, including Porphyromonas, Streptococcus, and Aggregatibacter actinomycetemcomitans. All bacteria were scraped out with a surgical blade, and the genomic DNA of the standard strain was extracted using a bacterial genomic DNA extraction kit. The reactions of different strains and strain-specific primers during the PCR detection process were observed, the products were cut and recovered, the DNA segments were extracted, and PCR amplification was performed to measure the amount of pathogens on the brackets.

[0108] 4. Western blot detection of changes in harmful products of plaques: mainly detect harmful products of the entire flora, LPS, IL-1β and TNF-α. The lysate was subjected to SDS-PAGE gel electrophoresis. The primary antibody was incubated in TBST at 4°C overnight. The membrane was washed with TBST and incubated with HRP secondary antibody at room temperature, and then washed with TBST. Visual analysis was then performed, and the blot was analyzed using the immunoblotting detection system (Biorad).

[0109] Second, use the composition of the present invention to treat bracket accessories.

[0110] Here’s how to use it:

[0111] Step 1: Rinse the dirt, blood, saliva, soft scale, etc. on the accessories with deionized water.

[0112] Step 2: Place the accessory in composition 1 for 3 minutes and rinse with deionized water.

[0113] Step 3: Place the attachment in composition 2 for treatment for 5 minutes.

[0114] Step 4: Rinse with deionized water and use a dental three-way air gun for 10 seconds.

[0115] Step 5: Place the treated accessories in an ultrasonic cleaner for a final oscillation rinse for 2 minutes.

[0116] The treated bracket attachments are tested again using the detection method. The main test items are as in the second part of this example, including: atomic force microscopy to show plaque thickness, surface morphology microscopy to detect the surface morphology of the flora, PCR to detect the amount of plaque, and Western blot to detect changes in harmful products of plaque.

[0117] The comparison diagram of the overall plaque thickness before treatment (left figure) and the overall plaque thickness after treatment with the composition for inhibiting pathogenic bacteria on the surface of oral attachments of Example 1 of the present invention (right figure) is as follows Figure 4 As shown, it is shown that the plaque thickness of the treatment group of the present invention is significantly reduced, indicating that the composition of the present invention can thoroughly clean the thickness of the bacterial colony.

[0118] Figure 5 It shows that the composition of the present invention can effectively eliminate the amount of bacterial colonization of Porphyromonas; Figure 6 This indicates that the composition of the present invention destroys the nutrient transport (hyphae) outside the bacteria and reduces the amount of 3 specific oral bacteria (see Appendix Figure 7 ), and the harmful products of bacteria, including LPS, IL-1β and TNF-α, were significantly inhibited and eliminated (see Appendix Figure 8 ), disintegrating the collagen fiber scaffold on the surface of the bracket (refer to the attached Fig. 9 ).

[0119] In summary, one or more technical solutions of the embodiments of the present invention have at least the following advantages:

[0120] 1. Punicalagin and myrtenol only have significant bactericidal effects within the specific concentration range of this patent.

[0121] 2. The effect of using punicalagin and myrtenol together is 10.53-17.7 times higher than that of using punicalagin alone or myrtenol alone. It was found that their specific combination has better effect.

[0122] 3. Myrtle alcohol destroys the collagen scaffold on the surface of oral attachments, destroying the living environment outside the bacteria.

[0123] 4. Specifically targets 3 types of bacteria on oral appendages.

[0124] 5. Other sterilization methods kill bacteria themselves and reduce the amount of bacteria. However, the nutrient transport (hyphae) outside the bacteria is not disrupted. The present invention can also specifically disrupt the nutrient transport channel (hyphae) outside the bacteria.

[0125] Finally, it should be noted that the terms "comprises," "includes," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements that are inherent to such process, method, article, or apparatus.

[0126] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0127] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A composition for inhibiting pathogenic bacteria on the surface of oral attachments, characterized in that: The composition for inhibiting pathogenic bacteria on the surface of oral attachments comprises composition 1 and composition 2 which are stored separately. The composition 1 comprises, by mass fraction, 1-2% punicalagin, 9-11% malic acid, 2-3% cellobiase, 2-3% celloglucanase, 3-5% sodium lauryl sulfate, 15-20% disodium ethylenediaminetetraacetic acid, 1-3% glycerol, and the balance is deionized water; The composition 2 comprises, by mass fraction, 0.1-0.5% myrtenol, 1-3% glycerol, 3-5% ethanol, 4-7% propylene glycol, 1-2% borax, and the balance is deionized water.

2. A composition for inhibiting pathogenic bacteria on the surface of oral attachments according to claim 1, characterized in that: The composition 1 comprises, by mass fraction, 1.5% punicalagin, 10% malic acid, 2.5% cellobiase, 2.5% celloglucanase, 4% sodium lauryl sulfate, 17.5% disodium ethylenediaminetetraacetic acid, 2% glycerol, and the balance is deionized water; The composition 2 comprises, by mass fraction, 0.25% myrtenol, 2% glycerol, 4% ethanol, 5% propylene glycol, 1.5% borax, and the balance is deionized water.

3. A method for preparing a composition for inhibiting pathogenic bacteria on the surface of oral attachments according to any one of claims 1-2, characterized in that: The method comprises: Firstly, the components of the composition 1 are weighed, and dissolving disodium ethylenediaminetetraacetate into deionized water; then sodium lauryl sulfate is added to dissolve, and then punicalagin, malic acid, cellobiase and celloglucanase are added to dissolve, so as to obtain the composition 1; Firstly, the components of the composition 2 are weighed, glycerin is dissolved in deionized water, and then ethanol, propylene glycol, borax and myrtenol are added in sequence to dissolve, so as to obtain the composition 2.

4. The preparation method according to claim 3, characterized in that: The dissolution temperature of the disodium ethylenediaminetetraacetic acid in deionized water is set at 50-60°C; then the temperature is lowered to 38-42°C at room temperature, sodium lauryl sulfate is added to dissolve, and then the temperature is lowered to 28-32°C at room temperature, and punicalagin, malic acid, cellobiase and celloglucanase are added to dissolve.

5. The preparation method according to claim 3, characterized in that: The temperature for dissolving glycerol into deionized water is 20-30°C.

6. A method for processing an accessory for wearing in the oral cavity, characterized in that: The method comprises: The accessory for wearing in the oral cavity is sequentially cleaned with deionized water, immersed in the composition 1 of claim 1, rinsed with deionized water, immersed in the composition 2 of claim 1 and rinsed.

7. The method according to claim 6, characterized in that The flushing includes deionized water flushing, air gun flushing, ultrasonic cleaning and vibration flushing in sequence.

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