A growth-promoting plant growth regulator for forest trees, its preparation method and application

Abstract

The present invention discloses a growth-promoting plant growth regulator for forest trees, its preparation method and application, belonging to the technical field of plant growth regulators. The plant growth regulator of the present invention comprises the following raw materials in parts by weight: 5-7 parts of Scutellaria baicalensis Georgi ferment, 0.01-0.02 parts of agar oligosaccharide, 4-5 parts of salicylic acid, 1-2 parts of echinacea polysaccharide, 0.2-0.3 parts of potassium indolebutyrate, and 0.6-0.8 parts of sodium naphthylacetate. The plant growth regulator of the present invention is applied to Picea crassifolia Kom. The components in the regulator synergistically enhance the effect, which can significantly promote the growth of Picea crassifolia Kom. plants and improve the germination rate of Picea crassifolia Kom. seeds at the same time. The plant growth regulator of the present invention effectively solves the problems of slow growth and low wood yield of Picea crassifolia Kom., and the components are safe and environmentally friendly, which has a positive effect on reducing environmental pollution and reducing agricultural residues.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant growth regulators, and particularly relates to a growth-promoting plant growth regulator for forest trees, its preparation method and application. Background Art

[0002] Picea crassifolia Picea crassifolia kom. ) belongs to the genus Picea of the Pinaceae family, and is an evergreen arbor, mainly distributed in Qinghai, Gansu and other places. The growth rate of Picea crassifolia is relatively slow compared with other tree species, but it has strong adaptability, has relatively low requirements for the temperature and humidity of the growth environment, and can still grow normally in a cold, humid environment. Picea crassifolia is resistant to barrenness, can grow in neutral soil, is often used for desert control, and has the functions of wind prevention and sand fixation, preventing soil erosion, and improving the local ecological environment. The trunk of Picea crassifolia is straight and thick, and the wood is of good quality and is often used to make furniture. The kernel has high nutritional value and can be eaten. Due to its beautiful tree shape, Picea crassifolia is also often used as an ornamental tree species. In short, Picea crassifolia has high ecological value, economic value and ornamental value. However, the seeds of Picea crassifolia are small, and the thousand-grain weight is only about 4.5 g. During the sowing process, it has relatively high requirements for soil temperature and humidity. Cold, dry, compacted soil or high-temperature, high-humidity, waterlogging will affect the germination of seeds and the growth of seedlings.

[0003] Carrying out large-scale sand control and afforestation is one of the effective methods to improve land desertification. Large-scale planting of Picea crassifolia is of great significance for solving the problem of land desertification. However, how to accelerate the rapid growth of Picea crassifolia in desert soil is an urgent problem to be explored at present.

[0004] In forestry production, the height and diameter growth of seedlings, the rooting of cuttings, and the survival rate of afforestation are all key issues affecting seedling raising and forest cultivation. Plant growth regulators will play a certain metabolic role in plants, and achieve the purpose of strengthening seedlings by regulating plant growth and affecting the transfer of the center of plant growth. Therefore, plant growth regulators play an important role in increasing production and income in agriculture and forestry. Summary of the Invention

[0005] The main purpose of the present invention is to provide a growth-promoting plant growth regulator for forest trees, which can significantly improve the growth of Picea crassifolia plants and at the same time increase the germination rate of Picea crassifolia seeds.

[0006] To achieve the above technical purpose, the technical solution adopted by the present invention is as follows:

[0007] A growth-promoting plant growth regulator for forest trees, which is prepared from the following raw materials in parts by weight:

[0008] 5 - 7 parts of Scutellaria baicalensis Georgi ferment, 0.01 - 0.02 parts of agar oligosaccharide, 4 - 5 parts of salicylic acid, 1 - 2 parts of echinacea polysaccharide, 0.2 - 0.3 parts of indolebutyric acid potassium, 0.6 - 0.8 parts of sodium naphthylacetate.

[0009] Further, the preparation method of the Scutellaria baicalensis Georgi ferment is as follows:

[0010] S1: After drying and pulverizing the Scutellaria baicalensis Georgi root to obtain Scutellaria baicalensis Georgi powder, add a composite hydrolysis enzyme solution containing β - glucosidase and hemicellulase, enzymatically hydrolyze at 40 - 50 °C for 2 - 4 h, inactivate at high temperature, cool to room temperature, and reserve the mixture;

[0011] S2: Add Bacillus niabensis and Acinetobacter bereziniae to the mixture, ferment at 30 °C for 24 h, then filter, centrifuge the fermentation broth, take the supernatant, and dry to obtain the Scutellaria baicalensis Georgi ferment.

[0012] Furthermore, the mass - volume ratio of the Scutellaria baicalensis Georgi powder to the composite hydrolysis enzyme solution in step S1 is 1 kg: 2 L.

[0013] Even further, the composite hydrolysis enzyme solution contains 30 - 40 g / L of β - glucosidase and 12 - 18 g / L of hemicellulase.

[0014] Furthermore, the addition amount of Bacillus niabensis in step S2 is 10 - 15 g / L, and that of Acinetobacter bereziniae is 15 - 25 g / L.

[0015] Even further, the viable count of Bacillus niabensis is 1 billion / g, and the viable count of Acinetobacter bereziniae is 1 billion / g.

[0016] Furthermore, the strain number of Bacillus niabensis in step S2 is CGMCC1.16140, purchased from China General Microbiological Culture Collection Center, and the original preservation date is March 20, 2017; the strain number of Acinetobacter bereziniae is CGMCC1.12683, purchased from China General Microbiological Culture Collection Center, and the original preservation date is September 15, 2013.

[0017] A preparation method of a growth - promoting plant growth regulator for forest trees is prepared by the following steps:

[0018] Mix the Scutellaria baicalensis Georgi ferment, agar oligosaccharide, salicylic acid, echinacea polysaccharide, indolebutyric acid potassium, and sodium naphthylacetate evenly to obtain the plant growth regulator.

[0019] An application of a plant growth regulator in promoting the growth of forest trees.

[0020] Further, the forest tree is Picea crassifolia Kom.

[0021] The raw materials used in the present invention are all commercially available.

[0022] The regulator of the present invention uses Scutellaria baicalensis Georgi ferment, and the Scutellaria baicalensis Georgi ferment contains a variety of bioactive substances. In the present invention, the cell wall of Scutellaria baicalensis Georgi is destroyed by enzymatic hydrolysis to release more active ingredients; in addition, metabolites are produced by Bacillus niabensis and Acinetobacter bereziniae during the fermentation process, which have functions such as phosphorus solubilization and secretion of auxin indole acetic acid (IAA), etc., to promote plant growth.

[0023] The present invention adds Echinacea purpurea polysaccharide, which can provide carbon source and energy supply for plants, and can regulate the synthesis of hormones in plants, thereby regulating the growth rhythm of plants; in addition, it plays an important role in the nutrient absorption of plants. It can form an adhesion layer on the surface of the root system, increase the contact area between the root system and soil particles, and improve the nutrient absorption efficiency; in addition, it can also form complexes with some mineral elements, increase the availability of these elements in the soil, and promote the absorption of mineral elements by plants. The present invention adds agar oligosaccharide, which can stimulate plant growth to a certain extent and improve the stress resistance of plants.

[0024] Beneficial effects

[0025] The components in the regulator of the present invention act synergistically. On the one hand, it promotes root growth, improves the absorption of nutrients by the root system, and at the same time speeds up the cell division rate of plants, promoting plant growth; on the other hand, it regulates the water balance of plants, increases the drought tolerance of cells, and improves the stress resistance of plants to harsh environments such as drought; in addition, it can improve the germination rate of Picea crassifolia seeds. The plant growth regulator of the present invention can effectively solve the problems of slow growth and low wood yield of Picea crassifolia, and the components are safe and environmentally friendly, which has a positive effect on reducing environmental pollution and reducing agricultural residues. Description of the drawings

[0026] Figure 1 It is the phosphorus solubilization effect diagram of Bacillus niabensis and Acinetobacter bereziniae. Note: Figure A is Bacillus niabensis, and B is Acinetobacter bereziniae. Detailed implementation manners

[0027] The technical solutions of the present invention will be further described below in conjunction with specific embodiments, but not limited thereto.

[0028] Example 1

[0029] A growth-promoting plant growth regulator for forest trees is prepared from the following raw materials in parts by weight:

[0030] 5 parts of Scutellaria baicalensis Georgi ferment, 0.01 part of agar oligosaccharide, 4 parts of salicylic acid, 1 part of Echinacea purpurea polysaccharide, 0.2 part of indolebutyric acid potassium, and 0.6 part of sodium naphthylacetate.

[0031] The preparation method of the Scutellaria baicalensis Georgi ferment is as follows:

[0032] S1: After drying and pulverizing Scutellaria baicalensis roots to obtain Scutellaria baicalensis powder, add a composite hydrolysis enzyme solution containing β-glucosidase and hemicellulase, enzymatically hydrolyze at 40 - 50 °C for 2 - 4 h, inactivate at high temperature, cool to room temperature, and reserve the mixture for use;

[0033] S2: Add Bacillus niabensis and Acinetobacter bereziniae to the mixture, ferment at 30 °C for 24 h, then filter, centrifuge the fermentation broth, take the supernatant, and dry to obtain the Scutellaria baicalensis ferment.

[0034] In the step S1, the mass - volume ratio of Scutellaria baicalensis powder to the composite hydrolysis enzyme solution is 1 kg: 2 L.

[0035] The composite hydrolysis enzyme solution contains 30 g / L of β-glucosidase and 12 g / L of hemicellulase.

[0036] In the step S2, the addition amount of Bacillus niabensis is 10 g / L, and that of Acinetobacter bereziniae is 15 g / L.

[0037] The viable count of Bacillus niabensis is 1 billion per gram, and the viable count of Acinetobacter bereziniae is 1 billion per gram.

[0038] A preparation method of a growth-promoting plant growth regulator for forest trees is prepared by the following steps:

[0039] Mix the Scutellaria baicalensis ferment, agar oligosaccharide, salicylic acid, echinacea polysaccharide, potassium indolebutyrate, and sodium naphthylacetate evenly to obtain the plant growth regulator.

[0040] Example 2

[0041] A growth-promoting plant growth regulator for forest trees is prepared from the following raw materials by weight:

[0042] 6 parts of Scutellaria baicalensis ferment, 0.01 part of agar oligosaccharide, 5 parts of salicylic acid, 2 parts of echinacea polysaccharide, 0.2 part of potassium indolebutyrate, and 0.7 part of sodium naphthylacetate.

[0043] The preparation method of the Scutellaria baicalensis ferment is as follows:

[0044] S1: After drying and pulverizing Scutellaria baicalensis roots to obtain Scutellaria baicalensis powder, add a composite hydrolysis enzyme solution containing β-glucosidase and hemicellulase, enzymatically hydrolyze at 40 - 50 °C for 2 - 4 h, inactivate at high temperature, cool to room temperature, and reserve the mixture for use;

[0045] S2: Add Bacillus niabensis and Acinetobacter bereziniae to the mixture, ferment at 30 °C for 24 h, then filter, centrifuge the fermentation broth, take the supernatant, and dry to obtain the Scutellaria baicalensis ferment.

[0046] In the step S1, the mass-volume ratio of the Scutellaria baicalensis powder to the complex hydrolase solution is 1 kg: 2 L.

[0047] The complex hydrolase solution contains 35 g / L of β-glucosidase and 15 g / L of hemicellulase.

[0048] In the step S2, the addition amounts of Bacillus niabensis and Acinetobacter bereziniae are 12 g / L and 20 g / L respectively.

[0049] The viable count of Bacillus niabensis is 1 billion per gram, and the viable count of Acinetobacter bereziniae is 1 billion per gram.

[0050] A preparation method of a growth-promoting plant growth regulator for forest trees is prepared by the following steps:

[0051] Mix the Scutellaria baicalensis ferment, agar oligosaccharide, salicylic acid, echinacea polysaccharide, indolebutyric acid potassium salt, and sodium naphthylacetate evenly to obtain the plant growth regulator.

[0052] Example 3

[0053] A growth-promoting plant growth regulator for forest trees is prepared from the following raw materials by weight:

[0054] 7 parts of Scutellaria baicalensis ferment, 0.02 part of agar oligosaccharide, 5 parts of salicylic acid, 2 parts of echinacea polysaccharide, 0.3 part of indolebutyric acid potassium salt, and 0.8 part of sodium naphthylacetate.

[0055] The preparation method of the Scutellaria baicalensis ferment is as follows:

[0056] S1: After drying and crushing the Scutellaria baicalensis root to obtain Scutellaria baicalensis powder, add a complex hydrolase solution containing β-glucosidase and hemicellulase, carry out enzymatic hydrolysis at 40 - 50 °C for 2 - 4 h, inactivate at high temperature, cool to room temperature, and reserve the mixture;

[0057] S2: Add Bacillus niabensis and Acinetobacter bereziniae to the mixture, ferment at 30 °C for 24 h, then filter, centrifuge the fermentation broth, take the supernatant, and dry to obtain the Scutellaria baicalensis ferment.

[0058] In the step S1, the mass-volume ratio of the Scutellaria baicalensis powder to the complex hydrolase solution is 1 kg: 2 L.

[0059] The complex hydrolase solution contains 40 g / L of β-glucosidase and 18 g / L of hemicellulase.

[0060] In the step S2, the addition amounts of Bacillus niabensis and Acinetobacter bereziniae are 15 g / L and 25 g / L respectively.

[0061] The viable count of Bacillus niabensis is 1 billion per gram, and the viable count of Acinetobacter bereziniae is 1 billion per gram.

[0062] A preparation method of a growth-promoting plant growth regulator for forest trees is prepared by the following steps:

[0063] Mix the Scutellaria baicalensis Georgi ferment, agar oligosaccharide, salicylic acid, echinacea polysaccharide, potassium indolebutyrate, and sodium naphthylacetate evenly to obtain the plant growth regulator.

[0064] Comparative Example 1

[0065] A growth-promoting plant growth regulator for forest trees is prepared from the following raw materials by weight:

[0066] 7 parts of Scutellaria baicalensis Georgi ferment, 0.02 part of mannan oligosaccharide, 5 parts of salicylic acid, 2 parts of echinacea polysaccharide, 0.3 part of potassium indolebutyrate, and 0.8 part of sodium naphthylacetate.

[0067] The preparation method of the Scutellaria baicalensis Georgi ferment is as follows:

[0068] S1: After drying and crushing the Scutellaria baicalensis Georgi root to obtain Scutellaria baicalensis Georgi powder, add a composite hydrolysis enzyme solution containing β-glucosidase and hemicellulase, enzymatically hydrolyze at 40-50 °C for 2-4 h, inactivate at high temperature, cool to room temperature, and reserve the mixed solution;

[0069] S2: Add Neobacillus niabensis and Acinetobacter bereziniae to the mixed solution, ferment at 30 °C for 24 h, filter, centrifuge the fermentation broth, take the supernatant, and dry to obtain the Scutellaria baicalensis Georgi ferment.

[0070] In the step S1, the mass-volume ratio of the Scutellaria baicalensis Georgi powder to the composite hydrolysis enzyme solution is 1 kg: 2 L.

[0071] The composite hydrolysis enzyme solution contains 40 g / L of β-glucosidase and 18 g / L of hemicellulase.

[0072] In the step S2, the addition amount of Neobacillus niabensis is 15 g / L, and that of Acinetobacter bereziniae is 25 g / L.

[0073] The viable count of Neobacillus niabensis is 1 billion / g, and the viable count of Acinetobacter bereziniae is 1 billion / g.

[0074] A preparation method of a growth-promoting plant growth regulator for forest trees is prepared by the following steps:

[0075] Mix the Scutellaria baicalensis Georgi ferment, mannan oligosaccharide, salicylic acid, echinacea polysaccharide, potassium indolebutyrate, and sodium naphthylacetate evenly to obtain the plant growth regulator.

[0076] Compared with Example 3, in this comparative example, except that agar oligosaccharide is replaced by mannan oligosaccharide, the other raw materials and steps are the same as those in Example 3.

[0077] Comparative Example 2

[0078] A growth-promoting plant growth regulator for forest trees is prepared from the following raw materials by weight:

[0079] 7 parts of Scutellaria baicalensis Georgi ferment, 0.02 part of agar oligosaccharide, 5 parts of salicylic acid, 0.3 part of potassium indolebutyrate, and 0.8 part of sodium naphthylacetate.

[0080] The preparation method of the Scutellaria baicalensis Georgi ferment is as follows:

[0081] S1: After drying and pulverizing the Scutellaria baicalensis Georgi root to obtain Scutellaria baicalensis Georgi powder, add a composite hydrolase solution containing β-glucosidase and hemicellulase, enzymatically hydrolyze at 40 - 50 °C for 2 - 4 h, inactivate at high temperature, cool to room temperature, and reserve the mixture;

[0082] S2: Add Bacillus niabensis and Acinetobacter bereziniae to the mixture, ferment at 30 °C for 24 h, then filter, centrifuge the fermentation broth, take the supernatant, and dry to obtain the Scutellaria baicalensis Georgi ferment.

[0083] In step S1, the mass - volume ratio of Scutellaria baicalensis Georgi powder to the composite hydrolase solution is 1 kg:2 L.

[0084] The composite hydrolase solution contains 40 g / L of β-glucosidase and 18 g / L of hemicellulase.

[0085] In step S2, the addition amount of Bacillus niabensis is 15 g / L, and that of Acinetobacter bereziniae is 25 g / L.

[0086] The viable count of Bacillus niabensis is 1 billion / g, and the viable count of Acinetobacter bereziniae is 1 billion / g.

[0087] A preparation method of a growth-promoting plant growth regulator for forest trees is prepared by the following steps:

[0088] Mix the Scutellaria baicalensis Georgi ferment, agar oligosaccharide, salicylic acid, potassium indolebutyrate, and sodium naphthylacetate evenly to obtain the plant growth regulator.

[0089] Compared with Example 3, in this comparative example, except for not adding echinacea polysaccharide, the other raw materials and steps are the same as those in Example 3.

[0090] Comparative Example 3

[0091] A growth-promoting plant growth regulator for forest trees is prepared from the following raw materials by weight:

[0092] 7 parts of Scutellaria baicalensis Georgi extract, 0.02 part of agar oligosaccharide, 5 parts of salicylic acid, 2 parts of echinacea polysaccharide, 0.3 part of potassium indolebutyrate, and 0.8 part of sodium naphthylacetate.

[0093] The preparation method of the Scutellaria baicalensis Georgi extract is as follows:

[0094] After drying and pulverizing the Scutellaria baicalensis Georgi root to obtain Scutellaria baicalensis Georgi powder, a composite hydrolase solution containing β-glucosidase and hemicellulase is added, and enzymatic hydrolysis is carried out at 40-50 °C for 2-4 h, inactivated at high temperature, cooled to room temperature, centrifuged to obtain the supernatant, and dried to obtain the Scutellaria baicalensis Georgi extract.

[0095] In the step S1, the mass-volume ratio of the Scutellaria baicalensis Georgi powder to the composite hydrolase solution is 1 kg: 2 L.

[0096] The composite hydrolase solution contains 40 g / L of β-glucosidase and 18 g / L of hemicellulase.

[0097] A preparation method of a growth-promoting plant growth regulator for forest trees is prepared by the following steps:

[0098] The Scutellaria baicalensis Georgi extract, agar oligosaccharide, salicylic acid, echinacea polysaccharide, indolebutyric acid potassium salt, and sodium naphthylacetate are mixed evenly to obtain the plant growth regulator.

[0099] Compared with Example 3, in this comparative example, except that Scutellaria baicalensis Georgi is not fermented, the other raw materials and steps are the same as those in Example 3.

[0100] Comparative Example 4

[0101] A growth-promoting plant growth regulator for forest trees is prepared from the following raw materials by weight:

[0102] 0.02 parts of agar oligosaccharide, 5 parts of salicylic acid, 2 parts of echinacea polysaccharide, 0.3 part of indolebutyric acid potassium salt, and 0.8 part of sodium naphthylacetate.

[0103] A preparation method of a growth-promoting plant growth regulator for forest trees is prepared by the following steps:

[0104] The agar oligosaccharide, salicylic acid, echinacea polysaccharide, indolebutyric acid potassium salt, and sodium naphthylacetate are mixed evenly to obtain the plant growth regulator.

[0105] Compared with Example 3, in this comparative example, except that the Scutellaria baicalensis Georgi fermented product is not added, the other raw materials and steps are the same as those in Example 3.

[0106] Performance test

[0107] Determination of strain-related metabolites

[0108] Determination of phosphorus solubilization ability:

[0109] (1) Strain activation: After thawing Bacillus niabensis and Acinetobacter bereziniae, they are transferred to an LB medium and cultured at 30 °C for activation culture for 24-48 h to obtain activated strains of the two bacteria.

[0110] (2) Inoculate the strain onto the inorganic phosphorus solid medium and incubate it in a constant temperature incubator at 30 °C for 3 days, then observe the formation of a phosphorus solubilizing zone.

[0111] Determination of indole-3-acetic acid (IAA) production ability:

[0112] The Salkowski method was used to determine the IAA synthesis ability of the strain. First, inoculate the strain into the NA liquid medium containing 200 mg·L - 1 L-tryptophan, and incubate it in a shaker at 30 °C and 170 r / min for 3 days. Use a low-temperature centrifuge to centrifuge at 4 °C and 10000 r / min for 10 min, take out the supernatant and add an equal volume of Salkowski colorimetric solution. After standing and reacting in the dark for 30 min, use a UV spectrophotometer to measure the absorbance at a wavelength of 530 nm to determine the IAA synthesis ability of the strain. The data statistics are shown in Table 1.

[0113] Table 1 Determination of indole-3-acetic acid production ability

[0114]

[0115] Planting experiment

[0116] Experimental materials and methods

[0117] Experiment 1: The growth-promoting effect of the plant growth regulator of the present invention on Picea crassifolia Kom. plants.

[0118] The Picea crassifolia Kom. seedlings used in the experiment had consistent growth status and a plant height of about 50 cm, and were purchased from the local nursery market. The experiment was carried out in Haiguan County, Qinghai Province. The Picea crassifolia Kom. seedlings were randomly divided into 8 treatment groups: a control group (blank control) and 7 experimental groups using the plant growth regulators obtained in Examples 1-3 and Comparative Examples 1-4. Each treatment group had 20 plants, divided into 4 replicate groups.

[0119] The randomly divided Picea crassifolia Kom. seedlings were planted in sandy soil treated with carbendazim wettable powder, with a plant spacing of 3 m × 2 m. The planting pit specifications for Picea crassifolia Kom. seedlings were 40 cm × 35 cm and the planting depth was 25 cm. Unified management measures were taken for all Picea crassifolia Kom. The experiment started on March 1, 2023. Every about six weeks, the leaves were sprayed with the regulators of Examples 1-3 and Comparative Examples 1-4 diluted 300 times. When spraying, choose a windless day and spray until the two sides of the leaves are dripping. A total of 3 spraying treatments were carried out in mid-April, early June, and mid-July respectively. In September 2023, the new shoot growth, crown growth, ground diameter, new root growth, and lateral root growth of the seedlings were counted, and the data were recorded in Table 2.

[0120] Table 2 Growth status of Picea crassifolia Kom.

[0121]

[0122] As can be seen from Table 2, compared with the control group, the plant growth regulators of Examples 1-3 of the present invention can significantly increase the new shoot growth, crown width growth, new root growth and lateral root growth of Picea crassifolia, indicating that the plant growth regulator of the present invention has an obvious growth-promoting effect on Picea crassifolia plants. The growth-promoting effects of Comparative Examples 1-4 with changed regulator components are all weakened to varying degrees. Therefore, the components of the plant growth regulator of the present invention cooperate synergistically to promote the growth of Picea crassifolia, and none of them can be missing.

[0123] Experiment 2: The germination rate of Picea crassifolia seeds and the growth of seedlings by the plant growth regulator of the present invention

[0124] In early April of the following year after the seeds were harvested, a seed germination test and a determination of seedling growth were carried out. The Picea crassifolia seed test was set up with 8 treatment groups: a control group (blank control) and test groups treated with the plant growth regulators of Examples 1-3 and Comparative Examples 1-4 for seed dressing. The application amount of the plant growth regulator was 3% of the weight of Picea crassifolia seeds. 500 seeds were randomly selected from each of the 8 treatment groups and sown in the preset seedbed in the test field. The seed sowing density was 100 seeds / m 2 , and the emergence of the seeds breaking through the soil was regarded as germination. The germination rate was counted two months after sowing. Germination rate (%) = (number of seeds emerging through the soil / number of seeds sown) × 100; 20 Picea crassifolia seedlings were randomly selected from each group 120 days after sowing to measure the seedling height and root length.

[0125] Table 3 Performance of Picea crassifolia seeds under different treatments

[0126]

[0127] As can be seen from the data in Table 3, the plant growth regulator of the present invention can significantly increase the germination rate of Picea crassifolia seeds, and increase the seedling height and root length of Picea crassifolia seedlings, indicating that the regulator of the present invention can increase the biomass of seedlings. Therefore, the plant growth regulator of the present invention has a significant growth-promoting effect on Picea crassifolia seeds and seedlings.

[0128] It should be noted that the above-mentioned embodiments are only some of the preferred embodiments for implementing the present invention, rather than all embodiments. Obviously, based on the above-mentioned embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

Claims

1. Application of a growth-promoting plant growth regulator for forest trees in promoting the growth of Picea crassifolia, characterized in that, The growth promoter plant growth regulator for forest trees comprises the following raw materials in parts by weight: 5-7 parts of Scutellaria baicalensis Georgi ferment, 0.01-0.02 parts of agar oligosaccharide, 4-5 parts of salicylic acid, 1-2 parts of Echinacea purpurea polysaccharide, 0.2-0.3 parts of potassium indolebutyrate, 0.6-0.8 parts of sodium naphthylacetate; The preparation method of the Scutellaria baicalensis Georgi ferment is as follows: S1: After taking Scutellaria baicalensis Georgi roots, drying and pulverizing them to obtain Scutellaria baicalensis Georgi powder, adding a composite hydrolysis enzyme solution containing β-glucosidase and hemicellulase, enzymolyzing at 40-50 °C for 2-4 h, inactivating at high temperature, cooling to room temperature, and reserving the mixed solution; S2: Adding Bacillus niabensis and Acinetobacter bereziniae to the mixed solution, fermenting at 30 °C for 24 h, filtering, centrifuging the fermentation broth, taking the supernatant, and drying to obtain the Scutellaria baicalensis Georgi ferment; In the step S2, the strain number of Bacillus niabensis is CGMCC1.16140, purchased from China General Microbiological Culture Collection Center; the strain number of Acinetobacter bereziniae is CGMCC1.12683, purchased from China General Microbiological Culture Collection Center.

2. Use of the growth-promoting plant growth regulator for forest trees according to claim 1 in promoting the growth of Picea crassifolia, characterized in that, In the step S1, the mass-volume ratio of Scutellaria baicalensis Georgi powder to the composite hydrolysis enzyme solution is 1 kg:2 L.

3. Use of the growth-promoting plant growth regulator for forest trees according to claim 2 in promoting the growth of Picea crassifolia, characterized in that, The composite hydrolysis enzyme solution contains 30-40 g / L of β-glucosidase and 12-18 g / L of hemicellulase.

4. The application of the growth-promoting plant growth regulator for forest trees according to claim 1 in promoting the growth of Picea crassifolia, characterized in that, In the step S2, the addition amount of Bacillus niabensis is 10-15 g / L, and Acinetobacter bereziniae is 15-25 g / L.

5. Use of the growth-promoting plant growth regulator for forest trees according to claim 4 in promoting the growth of Picea crassifolia, characterized in that, The viable count of Bacillus niabensis is 1 billion / g, and the viable count of Acinetobacter bereziniae is 1 billion / g.

6. Use of the growth-promoting plant growth regulator for forest trees according to claim 1 in promoting the growth of Picea crassifolia, characterized in that, The preparation method of the growth promoter plant growth regulator for forest trees is prepared by the following steps: Mix the Scutellaria baicalensis Georgi ferment, agar oligosaccharide, salicylic acid, Echinacea purpurea polysaccharide, potassium indolebutyrate, and sodium naphthylacetate evenly to obtain the plant growth regulator.

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