Composition for improving color and brightness of fish body and application thereof

Abstract

The invention provides a composition for improving the color and brightness of a fish body and application thereof, the composition for improving the color and brightness of the fish body comprises propyl lutein and dehydroxanthophyll, and the mass ratio of propyl lutein to dehydroxanthophyll is (10-45): 1. According to the invention, propyl lutein and dehydroxanthophyll are compounded according to a specific ratio, so that the chroma of aquatic tissues can be obviously improved, especially the coloring effect on back and belly skins of fishes can be obviously improved, which cannot be achieved by single use or combined use of lutein, zeaxanthin and astaxanthin.

Description

Technical Field

[0001] The invention relates to the technical field of colorants, and in particular to a composition for improving the color and brightness of fish bodies and an application thereof. Background Art

[0002] As people's material life improves, the quality of artificially cultivated aquatic products has attracted more and more attention. The main factors affecting the market value and ornamental value of many aquatic products are their external morphology and body color. The colorful body color of aquatic animals is mostly affected by carotenoids, and because they do not have the ability to synthesize this pigment themselves, they must obtain it from food. However, with the intensive development of aquaculture, the scale of economic fish farming has expanded rapidly, and the breeding cycle has become shorter. After feeding a large amount of artificial compound feed, their body color will become lighter and lighter, and even abnormal phenomena will occur, which greatly reduces their commodity value.

[0003] To address this problem, by adding carotenoid colorants to feed during aquaculture, not only can the body color and meat quality of aquatic products be improved, but their own immunity can also be enhanced, thereby promoting the growth and reproduction of aquatic products. Patent applications with publication numbers CN115024444A (large yellow croaker, lutein), CN113519726 A (large yellow croaker, astaxanthin and zeaxanthin), CN108208472 A (large yellow croaker, lutein and astaxanthin), CN110250367A (yellow catfish, lutein), CN104171368A (blood parrot fish, astaxanthin), CN106306348A (parrot fish, astaxanthin), CN103875980 A (parrot fish, red cardinal), and CN105433046 A (parrot fish, astaxanthin) disclose that the main colorants in feeds for large yellow croaker, yellow catfish, parrot fish, etc. are lutein, astaxanthin, zeaxanthin, etc.

[0004] However, the above-mentioned colorants have not achieved the ideal coloring effect on improving the body color of aquatic products such as large yellow croaker. Therefore, it is of great significance to develop an economical, healthy and green colorant that can improve the body color of aquatic products such as large yellow croaker. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a composition for improving the color and brightness of fish bodies and its application. The composition of the present invention can further significantly improve the color and brightness of fish bodies, which is superior to the prior art.

[0006] In a first aspect, the present invention provides a composition for improving the color and brightness of fish, comprising propyl lutein and dehydrogenated lutein, wherein the mass ratio of the propyl lutein to the dehydrogenated lutein is 10 to 45:1.

[0007] Lutein, also known as plant lutein, is a carotenoid with a chemical formula of C40 H 56 O2, widely found in vegetables, fruits, flowers and other plants. Lutein has been widely used as a colorant in condiments, tobacco, cakes, candies and a variety of feed processing. Astaxanthin is a keto-carotenoid, chemically named 3,3′-dihydroxy-4,4′-diketo-β,β′-carotene. It is widely found in the biological world, especially in aquatic animals such as shrimp, crab, fish and feathers of birds, playing a role in coloring. However, using them alone or in combination in aquatic products has limited effect on improving the color of fish and cannot satisfy consumers well.

[0008] The present invention unexpectedly discovered that combining propyl lutein and dehydrogenated lutein in a specific ratio can significantly improve the color and brightness of the fish body, and the effect is better than the prior art.

[0009] Among them, the chemical formula of propyl lutein is C 43 H 62 O3, can be prepared by chemical reaction. Dehydrogenated lutein is 3'-dehydrogenated lutein, the chemical formula is C 40 H 54 O2, finished products are available on the market.

[0010] In some embodiments of the present invention, the mass ratio of the propyl lutein to the dehydrolutein is 10 to 35:1, preferably 10 to 30:1, and more preferably 10 to 25:1.

[0011] In some embodiments of the present invention, the composition for improving the color and brightness of fish body consists of propyl lutein and dehydrolutein.

[0012] In some embodiments of the present invention, the composition further comprises lutein, and the lutein exists in a monomer, ester or other stable form.

[0013] On the basis of the composition of propyl lutein and dehydrogenated lutein, the coloring effect of fish body is better when lutein is combined with propyl lutein and dehydrogenated lutein. However, the coloring effect is not ideal when lutein is combined with only one of propyl lutein and dehydrogenated lutein. Among them, lutein has poor stability and is more sensitive to light, heat, etc. Therefore, it can be added in the form of monomer, lutein ester or other stable forms.

[0014] In some embodiments of the present invention, the mass ratio of the propyl lutein to the lutein is 1:1-10, preferably 1:1-5, and more preferably 1:1-3.

[0015] In some embodiments of the present invention, the composition for improving the color and brightness of fish body is composed of propyl lutein, dehydrogenated lutein and lutein. Preferably, the mass ratio of the three is 10-25:1:10-75.

[0016] In the present invention, there is no special requirement for the mass ratio of trans-lutein in the lutein.

[0017] In a second aspect, the present invention provides a nutritional composition comprising any one of the above-mentioned compositions for improving the color and brightness of fish bodies.

[0018] In some embodiments of the present invention, the nutritional composition further comprises one or more of oil, vitamins, proteins, carbohydrates, carriers and adjuvants.

[0019] In the present invention, the term "nutritional composition" means a composition that provides nutrition to a subject.

[0020] Among them, the carrier and the auxiliary agent can be selected according to the specific needs of the nutritional composition. For example, when the nutritional composition is made into tablets, magnesium stearate is usually added as a glidant. For another example, when the nutritional composition is made into a suspension, water is generally added as a carrier, and auxiliary agents such as surfactants and antioxidants may be appropriately added.

[0021] In a third aspect, the present invention provides a feed additive comprising any one of the above-mentioned compositions for improving the color and brightness of fish bodies.

[0022] The feed additive is in the form of powder, liquid or microcapsule.

[0023] In a fourth aspect, the present invention provides an application of the feed additive in improving the color and brightness of fish, comprising: adding the feed additive to the daily feed of fish for feeding, wherein the composition for improving the color and brightness of fish accounts for 0.1%-0.2% by mass in the feed.

[0024] In some embodiments of the present invention, the composition for improving fish body color and brightness accounts for 0.15% by weight in the feed.

[0025] The composition of the present invention has the effect of improving the color and brightness of fish bodies, and thus can be used as a feed additive and added to the daily feed of farmed fish for feeding, so that their body color and brightness are improved to meet consumer preferences.

[0026] The fish of the present invention include common fish with yellow to red (such as yellow, orange-yellow, orange, orange-red, and red) bodies, such as large yellow croaker, yellow catfish, and parrot fish.

[0027] In some embodiments of the present invention, the fish is large yellow croaker.

[0028] The present invention provides a composition for improving the color and brightness of fish bodies and an application thereof. By compounding propyl lutein and dehydrogenated lutein in a specific ratio, the chromaticity of aquatic tissues can be significantly improved, especially the coloring effect on the back and abdominal skin of fish, which cannot be achieved by using lutein, zeaxanthin or astaxanthin alone or in combination.

[0029] Furthermore, propyl lutein, dehydrolutein and lutein are compounded in a specific ratio to achieve a better coloring effect.

[0030] It should be noted that the endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, and these numerical ranges should be regarded as specifically disclosed in this article.

[0031] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "specific implementations", or "some specific implementations" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] If no specific techniques or conditions are specified in the examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0034] In the following examples, propyl lutein was homemade; dehydrogenated lutein, lutein, zeaxanthin and astaxanthin were all commercially available.

[0035] The preparation process of propyl lutein of the present invention is as follows:

[0036] 1. Solution preparation: Add 100 μL of concentrated hydrochloric acid to 10 mL of n-propanol, mix thoroughly to obtain acidic propanol, and place in a 4°C refrigerator for later use.

[0037] 2. Sample preparation. Weigh 0.1 mg of lutein crystals and slowly add acidic propanol (about 5 mL) to it until the lutein crystals are completely dissolved. Place the mixture in a refrigerator overnight to react for 24 hours to ensure that the reaction is complete. Afterwards, add 10 mL of deionized water to the mixture to quench the reaction, and then add 10 mL of saturated sodium bicarbonate solution to neutralize the reaction product to obtain a mixture containing propyl lutein.

[0038] 3. Separation and purification. Add 100 mL of dichloromethane to the mixed solution (3) for liquid-liquid extraction. After sufficient shaking, let stand to separate the layers and collect the dichloromethane layer. Repeat the extraction three times and combine the three dichloromethane layers. Concentrate and dry in a cold water bath under vacuum to obtain the powder, which is propyl lutein.

[0039] Blank Group

[0040] The experiment randomly selected large yellow croakers with healthy size, specifications, physique and no diseases. A total of 90 fish. Before the formal experiment began, the large yellow croakers were placed in a temporary barrel for acclimation for 2 weeks. After the acclimation, 90 large yellow croakers with uniform specifications and health were selected, with an average weight of (20.35±2.38) g, and randomly distributed to 3 breeding barrels, 30 fish in each barrel, and 1 group of 3 barrels, and fed with blank group feed. No colorant was added to the blank group feed. The breeding cycle was 8 weeks, and the fish were fed twice a day at regular intervals. The control parameters of the breeding water during the experiment were: temperature 20.00-25.75℃, salinity 21.85%-26.10%, dissolved oxygen content 6.5-7.8, nitrite content <0.01g / mL, and ammonia nitrogen content <0.2g / mL. After the breeding experiment, 5 fish were selected for each repetition, and the body color test site was the section from the rear edge of the gill cover to the anus (5 sites were measured on the back and abdomen at equal intervals). Before the test, the surface moisture was wiped with filter paper and the colorimeter was corrected to zero. For the test of skin color parameters, the colorimeter was used to detect the brightness, redness and yellowness of the fish body in the dark and record them (taking the average value).

[0041] Example 1

[0042] This embodiment provides a composition for improving the color and brightness of fish, which is composed of propyl lutein and dehydrogenated lutein in a mass ratio of 45:1.

[0043] The composition was added to feed (same as blank group feed) as a feed additive, and the addition amount of the composition to the feed was 0.15 wt%. The feed after addition was used for testing.

[0044] The experiment randomly selected healthy and disease-free yellow croakers of size, specifications and physique. A total of 90 fish were selected. Before the formal experiment began, the yellow croakers were placed in a temporary barrel for acclimation for 2 weeks. After the acclimation, 90 yellow croakers with uniform specifications and health were selected, with an average weight of (20.35±2.38) g. They were randomly assigned to 3 breeding barrels, 30 fish in each barrel, and 3 barrels were a group. They were fed with the experimental group feed. The breeding cycle was 8 weeks, and they were fed twice a day at regular intervals. The control parameters of the breeding water during the experiment were: temperature 20.00-25.75℃, salinity 21.85%-26.10%, dissolved oxygen content 6.5-7.8, nitrite content <0.01g / mL, and ammonia nitrogen content <0.2g / mL. After the breeding experiment, 5 fish were selected for each repetition, and the section from the posterior edge of the gill cover to the anus of the yellow croaker was used as the body color test site (5 sites were measured on the back and abdomen at equal intervals). Before testing, wipe the surface moisture with filter paper and calibrate the colorimeter to zero. For the test of skin color parameters, use a colorimeter to detect the brightness, redness and yellowness of the fish body in the dark and record them.

[0045] Example 2

[0046] This embodiment provides a composition for improving the color and brightness of fish, which is composed of propyl lutein and dehydrogenated lutein in a mass ratio of 35:1.

[0047] The composition was added to feed (same as blank group feed) as feed additive, and the addition amount of the composition to feed was 0.15wt%. The feed after addition was used for test, and the test setting was the same as that of Example 1.

[0048] Example 3

[0049] This embodiment provides a composition for improving the color and brightness of fish, which is composed of propyl lutein and dehydrogenated lutein in a mass ratio of 25:1.

[0050] The composition was added to feed (same as blank group feed) as feed additive, and the addition amount of the composition to feed was 0.15wt%. The feed after addition was used for test, and the test setting was the same as that of Example 1.

[0051] Example 4

[0052] This embodiment provides a composition for improving the color and brightness of fish bodies, which is composed of propyl lutein and dehydrogenated lutein in a mass ratio of 10:1.

[0053] The composition was added to feed (same as blank group feed) as feed additive, and the addition amount of the composition to feed was 0.15wt%. The feed after addition was used for test, and the test setting was the same as that of Example 1.

[0054] Example 5

[0055] This embodiment provides a composition for improving the color and brightness of fish, which is composed of propyl lutein, dehydrolutein and lutein in a mass ratio of 10:1:10.

[0056] The composition was added to feed (same as blank group feed) as feed additive, and the addition amount of the composition to feed was 0.15wt%. The feed after addition was used for test, and the test setting was the same as that of Example 1.

[0057] Example 6

[0058] This embodiment provides a composition for improving the color and brightness of fish, which is composed of propyl lutein, dehydrolutein and lutein in a mass ratio of 25:1:25.

[0059] The composition was added to feed (same as blank group feed) as feed additive, and the addition amount of the composition to feed was 0.15wt%. The feed after addition was used for test, and the test setting was the same as that of Example 1.

[0060] Example 7

[0061] This embodiment provides a composition for improving the color and brightness of fish bodies, which is composed of propyl lutein, dehydrolutein and lutein in a mass ratio of 35:1:35.

[0062] The composition was added to feed (same as blank group feed) as feed additive, and the addition amount of the composition to feed was 0.15wt%. The feed after addition was used for test, and the test setting was the same as that of Example 1.

[0063] Example 8

[0064] This embodiment provides a composition for improving the color and brightness of fish bodies, which is composed of propyl lutein, dehydrolutein and lutein in a mass ratio of 45:1:45.

[0065] The composition was added to feed (same as blank group feed) as feed additive, and the addition amount of the composition to feed was 0.15wt%. The feed after addition was used for test, and the test setting was the same as that of Example 1.

[0066] Example 9

[0067] This embodiment provides a composition for improving the color and brightness of fish bodies, which is composed of propyl lutein, dehydrolutein and lutein in a mass ratio of 10:1:100.

[0068] The composition was added to feed (same as blank group feed) as feed additive, and the addition amount of the composition to feed was 0.15wt%. The feed after addition was used for test, and the test setting was the same as that of Example 1.

[0069] Example 10

[0070] This embodiment provides a composition for improving the color and brightness of fish bodies, which is composed of propyl lutein, dehydrolutein and lutein in a mass ratio of 10:1:50.

[0071] The composition was added to feed (same as blank group feed) as feed additive, and the addition amount of the composition to feed was 0.15wt%. The feed after addition was used for test, and the test setting was the same as that of Example 1.

[0072] Comparative Example 1

[0073] This comparative example provides a colorant containing only lutein.

[0074] The colorant was added to the feed (same as the blank group feed) at an addition amount of 0.15 wt %. The feed after addition was used for the test, and the test setting was the same as that of Example 1.

[0075] Comparative Example 2

[0076] This comparative example provides a colorant containing only zeaxanthin.

[0077] The colorant was added to the feed (same as the blank group feed) at an addition amount of 0.15 wt %. The feed after addition was used for the test, and the test setting was the same as that of Example 1.

[0078] Comparative Example 3

[0079] This comparative example provides a colorant containing only astaxanthin.

[0080] The colorant was added to the feed (same as the blank group feed) at an addition amount of 0.15 wt %. The feed after addition was used for the test, and the test setting was the same as that of Example 1.

[0081] Comparative Example 4

[0082] This comparative example provides a colorant composition consisting of lutein and zeaxanthin in a mass ratio of 16:1.

[0083] The colorant composition was added to feed (same as blank group feed) at an addition amount of 0.15 wt %. The feed after addition was used for testing, and the test setting was the same as that of Example 1.

[0084] Comparative Example 5

[0085] This comparative example provides a colorant composition consisting of lutein and zeaxanthin in a mass ratio of 10:7.

[0086] The colorant composition was added to feed (same as blank group feed) at an addition amount of 0.15 wt %. The feed after addition was used for testing, and the test setting was the same as that of Example 1.

[0087] Comparative Example 6

[0088] This comparative example provides a colorant composition consisting of lutein, zeaxanthin and astaxanthin in a mass ratio of 16:1:2.

[0089] The colorant composition was added to feed (same as blank group feed) at an addition amount of 0.15 wt %. The feed after addition was used for testing, and the test setting was the same as that of Example 1.

[0090] Comparative Example 7

[0091] This comparative example provides a colorant composition consisting of lutein, zeaxanthin and astaxanthin in a mass ratio of 10:7:2.

[0092] The colorant composition was added to feed (same as blank group feed) at an addition amount of 0.15 wt %. The feed after addition was used for testing, and the test setting was the same as that of Example 1.

[0093] Comparative Example 8

[0094] This comparative example provides a colorant composition consisting of lutein, zeaxanthin and astaxanthin in a mass ratio of 10:7:2.

[0095] The colorant composition was added to feed (same as blank group feed) at an addition amount of 0.3 wt %. The feed after addition was used for testing, and the test setting was the same as that of Example 1.

[0096] Comparative Example 9

[0097] This comparative example provides a colorant composition, which is composed of propyl lutein and dehydrolutein in a mass ratio of 1:45.

[0098] The colorant composition was added to feed (same as blank group feed) at an addition amount of 0.15 wt %. The feed after addition was used for testing, and the test setting was the same as that of Example 1.

[0099] Comparative Example 10

[0100] This comparative example provides a colorant composition, which consists of propyl lutein and lutein in a mass ratio of 10:1.

[0101] The colorant composition was added to feed (same as blank group feed) at an addition amount of 0.15 wt %. The feed after addition was used for testing, and the test setting was the same as that of Example 1.

[0102] Comparative Example 11

[0103] This comparative example provides a colorant composition, which consists of lutein and dehydrolutein in a mass ratio of 10:1.

[0104] The colorant composition was added to feed (same as blank group feed) at an addition amount of 0.15 wt %. The feed after addition was used for testing, and the test setting was the same as that of Example 1.

[0105] Results The coloration of the fish bodies of the embodiment, blank group and comparative example is shown in Table 1.

[0106] Table 1

[0107]

[0108] As can be seen from Table 1, the L and b values ​​of the back and abdomen of the large yellow croaker in Examples 1 to 10 increased, indicating that the color and brightness of the large yellow croaker were significantly improved. In Examples 1-4, the ratio of propyl lutein to dehydrogenated lutein in Example 4 was 10:1, which was better than Example 3, Example 2, and Example 1 in coloring the body color of the large yellow croaker; in Examples 5-10, increasing the proportion of lutein in the composition did not achieve the effect of increasing coloring; in Examples 5 and 6, the ratio of propyl lutein, dehydrogenated lutein and lutein was in the optimal range of the present invention, and the L and b values ​​of the back and abdomen of the large yellow croaker were the highest, indicating the best coloring effect; and in Examples 9 to 10, when the ratio of propyl lutein to dehydrogenated lutein was in the most preferred range of the present invention, the ratio of lutein to propyl lutein was increased, and the L and b values ​​of the back and abdomen of the large yellow croaker were higher, but the effect was weaker than that of Examples 5 and 6. In Comparative Examples 1-11, the colorant used has a coloring effect on large yellow croaker, but the L and b values ​​of the large yellow croaker back and belly at the same addition amount are lower than those in Examples 1 to 10. In summary, the composition of the present invention has a significantly excellent coloring effect on the body color of large yellow croaker compared with other colorants.

[0109] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition for improving the color and brightness of fish, characterized in that: It comprises propyl lutein and dehydrogenated lutein, and the mass ratio of the propyl lutein to the dehydrogenated lutein is 10-45:

1.

2. The composition for improving the color and brightness of fish according to claim 1, characterized in that: The mass ratio of the propyl lutein to the dehydrolutein is 10 to 35:1, preferably 10 to 30:1, and more preferably 10 to 25:

1.

3. The composition for improving the color and brightness of fish according to claim 1 or 2, characterized in that: The composition also includes lutein, which is present in monomer, ester or other stable forms.

4. The composition for improving the color and brightness of fish according to claim 3, characterized in that: The mass ratio of the propyl lutein to the lutein is 1:1-10, preferably 1:1-5, and more preferably 1:1-3.

5. A nutritional composition, characterized in that The invention comprises the composition for improving the color and brightness of fish body as described in any one of claims 1 to 4.

6. The nutritional composition according to claim 5, characterized in that The nutritional composition further comprises one or more of oil, vitamins, proteins, carbohydrates, carriers and adjuvants.

7. A feed additive, characterized in that: The invention comprises the composition for improving the color and brightness of fish body as described in any one of claims 1 to 4.

8. The feed additive according to claim 7, characterized in that The feed additive is in the form of powder, liquid or microcapsule.

9. Use of the feed additive according to claim 7 or 8 for improving the color and brightness of fish, characterized in that: The feed additive is added to the daily feed of fish for feeding, and the composition for improving the color and brightness of the fish body accounts for 0.1%-0.2% by weight in the feed.

10. The use according to claim 9, characterized in that: The fish is large yellow croaker.

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