Flavor-modifying preparation method of Wanchu granules

Abstract

The invention discloses a flavoring preparation method of Wanchu granules, and belongs to the field of medicine preparation, and the flavoring preparation method comprises the following steps: S1, forming first mixed powder from rhizoma picrorhizae, rhizoma coptidis, catechu and fragrant ink; s2, in-vitro cultured calculus bovis is mixed with the first mixed powder to form second mixed powder; s3, forming bear gall powder liquid; s4, forming ox gall paste; s5, forming a third mixture from the ox gall paste and the bear gall powder liquid; s6, mixing the rape flower honey filtrate, the second mixed powder and the third mixture, granulating through a granulator, drying and finishing; and S7, mixing the borneol and artificial musk particles obtained in the step S6. According to the invention, not only is the taste modifying effect of the Wanchu granules greatly improved, but also the heat clearing effect of the Wanchu granules is improved.

Description

Technical Field

[0001] The invention relates to medicine preparation, and in particular to a method for preparing flavor-correcting Wanying granules. Background Art

[0002] Wanying medicines are available in the form of Wanying capsules, Wanying tablets and Wanying granules. The functions and indications of Wanying medicines are to clear away heat, calm nerves and detoxify. Among them, Wanying granules are used for children with high fever, irritability, mouth sores, and sore gums and throat.

[0003] CN115192652A discloses a Wanying granule and a preparation method thereof. The prescription of the Wanying granule in Example 1 is 60 parts by weight of coptis root, 60 parts by weight of picrorhizome, 60 parts by weight of catechu, 120 parts by weight of fragrant ink, 3.6 parts by weight of borneol, 12 parts by weight of bear bile powder, 3 parts by weight of artificial musk, 3 parts by weight of in vitro cultured bezoar, and 96 parts by weight of ox bile. In the preparation, powdered sugar (specifically powdered sucrose) is used as a flavoring agent for flavoring. Although powdered sugar is added to CN115192652A for flavoring, there is still room for further improvement in the effect. In addition, how to improve the effect of drugs is a permanent pursuit of pharmaceutical companies.

[0004] The above background technology is for facilitating the understanding of the present invention and is not the known technology disclosed to the general public before the application of the present invention. Summary of the invention

[0005] In view of the above-mentioned defects, the present invention provides a method for preparing the flavor correction of Wanying granules, which aims to improve at least one of the problems mentioned in the background technology.

[0006] The technical solution is: a method for preparing the flavor correction of Wanying granules, comprising the following steps: S1. The picrorhizon, coptis root, catechu, fragrant ink are crushed and stirred to form a first mixed powder; S2. The in vitro cultured bezoar and the first mixed powder in S1 were stirred and mixed to obtain a second mixed powder; S3. Add water to the bear bile powder and boil to dissolve the bear bile powder to obtain a bear bile powder liquid; S4. Concentrating and filtering the ox bile to obtain ox bile paste; S5. Add water to the ox bile paste and the bear bile powder and mix to obtain a third mixture; S6. The rapeseed nectar filtrate is added to the second mixed powder and stirred and mixed, and then the third mixture is added, and then 80~95 v / v% ethanol aqueous solution is added, stirred, and then granulated by a granulator, dried, and granulated; S7. Grind the borneol and artificial musk and sieve them and mix them with the sieved particles in S6; Among them, 50-65 weight parts of coptis root, 50-65 weight parts of picrorhizome, 50-65 weight parts of catechu, 105-125 weight parts of fragrant ink, 3-4 weight parts of borneol, 10.2-12 weight parts of bear bile powder, 2-3.5 weight parts of artificial musk, 2-3.5 weight parts of in vitro cultured bezoar, and 80-98 weight parts of ox bile; The rapeseed honey filtrate is a filtrate obtained by mixing 5-15 v / v% ethanol aqueous solution and rapeseed honey for extraction and filtration, wherein the 5-15 v / v% ethanol aqueous solution is 180-250 parts by weight and the rapeseed honey is 280-675 parts by weight; Herein, parts by weight are one of grams, tons, kilograms, thousands of kilograms or ten thousand kilograms.

[0007] Furthermore, in the preparation of the rapeseed nectar filtrate, the extraction temperature is 30-40° C. and the extraction time is 1-3 hours.

[0008] Furthermore, the 5-15 v / v% ethanol aqueous solution comprises 200-220 parts by weight and the rapeseed honey comprises 300-350 parts by weight.

[0009] Furthermore, the invention comprises 60 parts by weight of coptis root, 60 parts by weight of picrorhizome, 60 parts by weight of catechu, 120 parts by weight of scented ink, 3.6 parts by weight of borneol, 12 parts by weight of bear bile powder, 3 parts by weight of artificial musk, 3 parts by weight of in vitro cultured bezoar, 96 parts by weight of ox bile, 210 parts by weight of 5-15 v / v% aqueous ethanol solution, and 320 parts by weight of rapeseed honey.

[0010] Furthermore, the 80-95 v / v% ethanol aqueous solution is 180-250 parts by weight; in S3, the amount of water is 20-35 parts by weight; in S5, the amount of water is the amount of water evaporated during the concentration in S4.

[0011] Furthermore, the particle size of the first mixed powder is 170-190 μm, and the particle size of the in vitro cultured bezoar is 170-190 μm.

[0012] Furthermore, the drying temperature is 50-60° C., the granulation is performed by installing a 10-mesh stainless steel screen in a swing-type granulator, and the sieving is performed by installing a 10-mesh and 80-mesh stainless steel screen in a swing-type granulator.

[0013] Compared with the prior art, the present invention has the following beneficial effects: The applicant of the present invention discovered during the research and development of flavor improvement of Wanying granules that acacia honey or rapeseed honey dissolved and filtered in water or ethanol aqueous solution as a flavoring agent greatly improved the flavoring effect of Wanying granules.

[0014] The applicant of the present invention surprisingly discovered during the research and development of flavor improvement of Wanying Granules that the heat-clearing effect of Wanying Granules can be enhanced by adding rapeseed nectar filtered from a specific range of ethanol-water solution. DETAILED DESCRIPTION

[0015] As used herein: "Prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0016] The conjunction "consisting of excludes any unspecified element, step, or component. If used in a claim, this phrase renders the claim closed-ended so that it does not include materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of" appears in a clause of the body of a claim rather than immediately following the subject matter, it limits only the elements described in that clause; other elements are not excluded from the claim as a whole.

[0017] When an amount, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper preferred values ​​and lower preferred values, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted as including ranges "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described in this article, unless otherwise stated, the range is intended to include its end values ​​and all integers and fractions within the range.

[0018] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] Those skilled in the art will appreciate that the following examples are only used to illustrate the present application and should not be considered as limiting the scope of the present application. If specific conditions are not specified in the examples, they are carried out according to normal conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0020] In these examples, parts and percentages are by mass unless otherwise indicated.

[0021] "Parts by weight" refers to the basic unit of measurement for expressing the mass ratio of multiple components. 1 part can represent any unit mass, such as 1g or 2.689g. If we say that the mass of component A is a parts and the mass of component B is b parts, it means the ratio of the mass of component A to the mass of component B is a:b. Alternatively, it means that the mass of component A is aK and the mass of component B is bK (K is an arbitrary number, indicating a multiple factor). It should not be misunderstood that, unlike the mass parts, the sum of the mass parts of all components is not limited to 100 parts.

[0022] “And / or” is used to indicate that one or both of the stated situations may occur, for example, A and / or B includes (A and B) and (A or B).

[0023] In the following embodiments and comparative examples of the present invention, except for those prepared by ourselves, all others are commercially available.

[0024] In the present invention, water is purified water, and ethanol is medicinal ethanol.

[0025] Example 1 Take the sucrose (acacia honey or rapeseed honey) in Table 1 purchased from the market, add it into a container, add it into the ethanol aqueous solution in Table 1, stir, control the temperature at 30-40°C, extract for 2 hours, and then filter to obtain the filtrate.

[0026] Table 1 Filtrate 1-Filtrate 7 In Table 1, the particle size of sucrose powder is 170 μm Example 2 A method for preparing Wanying granules comprises the following steps: S1. 60 parts by weight of Picrorhizoma Coptidis, 60 parts by weight of Coptis chinensis, 60 parts by weight of Catechu, and 120 parts by weight of fragrant ink are crushed and stirred to form a first mixed powder, wherein the particle size of the first mixed powder is 175 μm.

[0027] S2. Stir and mix 3 parts by weight of in vitro cultured bezoar with the first mixed powder in S1 to obtain a second mixed powder, wherein the particle size of the in vitro cultured bezoar is 175 μm.

[0028] S3. Add 12 parts by weight of bear bile powder to 30 parts by weight of water and boil to dissolve to obtain bear bile powder liquid.

[0029] S4. Concentrate and filter 96 parts by weight of ox bile to obtain ox bile paste.

[0030] S5. Add an appropriate amount of water into the ox bile paste and the bear bile powder liquid and mix them to obtain a third mixture.

[0031] S6. Add the filtrate 2 to the second mixed powder and stir and mix, add the third mixture after mixing, add 238 parts by weight of ethanol aqueous solution (ethanol volume fraction is 80%), stir for 20 minutes, and then use a granulator to make granules, dry, granulate, and sieve. In this step, the drying temperature is 55°C, and the granulator is installed with a 10-mesh stainless steel screen for granulation, and the 10-mesh and 80-mesh stainless steel screens are installed for sieving.

[0032] S7. Grind 3.6 parts by weight of borneol and 3 parts by weight of artificial musk, sieve them through a 60-mesh sieve, mix them with the sieved particles in S6, and pack them into bags to obtain 1 g of Wanying granules per bag.

[0033] In S5, the appropriate amount of water is the amount of water evaporated during the concentration in S4.

[0034] Example 3 The only difference from Example 2 is that in this embodiment, in S6, the filtrate is filtrate 3.

[0035] Example 4 The only difference from Example 2 is that in this embodiment, in S6, the filtrate is filtrate 4.

[0036] Example 5 The only difference from Example 2 is that in this example, in S6, the filtrate is filtrate 1.

[0037] Example 6 The only difference from Example 2 is that in this embodiment, in S6, the filtrate is filtrate 5.

[0038] Example 7 The only difference from Example 2 is that in this embodiment, in S6, the filtrate is filtrate 6.

[0039] Comparative Example 1 The only difference from Example 2 is that in this comparative example, in S6, 675 parts by weight of sucrose powder are used to replace filtrate 2, wherein the particle size of the sucrose powder is 170 μm.

[0040] Comparative Example 2 The only difference from Example 2 is that in this comparative example, in S6, the filtrate is filtrate 7.

[0041] Comparative Example 3 The only difference from Example 2 is that in this comparative example, in S6, 675 parts by weight of acacia honey are used to replace filtrate 2.

[0042] Comparative Example 4 The only difference from Example 2 is that in this comparative example, in S6, 320 parts by weight of acacia honey are used to replace filtrate 2.

[0043] Comparative Example 5 The only difference from Example 2 is that in this comparative example, in S6, 320 parts by weight of rapeseed nectar are used to replace filtrate 2.

[0044] Example 8 The drugs of Examples 2 to 7 and Comparative Examples 1 to 5 were prepared into solutions with a concentration of 0.05 g / ml.

[0045] SD mice, 18-22 g, half male and half female, were divided into 12 groups, 10 mice in each group, and each group was housed in a cage. After one week of adaptive feeding, the corresponding drug solution was given by gavage, and the nausea and vomiting of the mice were observed 5 minutes and 20 minutes after taking the medicine, as shown in Table 2.

[0046] The 12 groups corresponded to the control group, Example 2 to Example 7, and Comparative Example 1 to Comparative Example 5. The control group was intragastrically administered with 0.5 ml / 10 g of normal saline, and the other groups were intragastrically administered with a dose of 0.5 ml / 10 g.

[0047] Table 2 is a comparison of vomiting reactions of mice in each group (n=1) Comparison of Comparative Example 3 with Comparative Example 1 shows that, in the same amount, acacia honey has a better flavor correction effect than powdered sugar (sucrose); Comparison of Comparative Example 4 and Comparative Example 5 with Comparative Example 3 shows that the flavor correction effect drops sharply after the addition amount of acacia honey and rapeseed honey is reduced by nearly half, and the flavor correction result is not ideal; Comparison of Example 7 with Comparative Example 4 shows that the acacia honey dissolved and filtered by 10% ethanol greatly improves the flavor correction effect, and the flavor correction effect is even better than that of Comparative Example 1; Comparison of Example 2-Example 6 and Comparative Example 5 shows that the ethanol aqueous solution or the rapeseed honey dissolved and filtered by water greatly improves the flavor correction effect, and the flavor correction effect is even better than that of Comparative Example 1, wherein the flavor correction effect decreases with the increase of ethanol content. Comparison of Comparative Example 2 with Comparative Example 1 shows that although the powdered sugar dissolved and filtered by 10% ethanol has a certain improvement over the undissolved and filtered one, the improvement effect is far less than that of acacia honey or rapeseed honey.

[0048] Example 9 Take 0.15 g of each of the drugs of Example 2 to Example 7 and Comparative Example 1 to Comparative Example 5, and dissolve them in water to form drug solutions for later use.

[0049] Rabbits weighing 2.5 kg were selected. They were deprived of water for 15 hours before the test. Normal rectal temperature was measured twice with an interval of 1 hour. Rabbits with a temperature difference of no more than 0.3°C were selected, and typhoid vaccine was injected into the marginal ear vein at a volume of 0.3 ml / kg. The body temperature was measured 1 hour later. 66 rabbits with fever whose temperature rose by 0.8°C~1°C were selected and divided into 11 groups. The prepared drug solutions were taken respectively, and the body temperature of each rabbit was tested 2h and 5h after taking the solution. The temperature change of each rabbit was calculated, and then the average value of each group was taken. The ratio of the average temperature drop of each group to the average temperature rise of each group (i.e. ratio = average temperature drop / average temperature rise) was calculated. The results are shown in Table 3.

[0050] Table 3 Heat-clearing effect after administration In Table 3, a ratio of 1 indicates that the group is completely cooled down, the farther away from 1, the worse the cooling effect, and the closer to 1, the better the cooling effect.

[0051] Comparison of Comparative Examples 1 to 5, Example 7, and Example 2 shows that for powdered sugar (sucrose), the overall heat-clearing effect is basically the same after filtration with ethanol aqueous solution and without filtration with ethanol aqueous solution; for rapeseed honey, the rapeseed honey filtered with ethanol aqueous solution has a better overall heat-clearing effect than the rapeseed honey filtered with ethanol aqueous solution; but for acacia honey, although it has the same heat-clearing function as rapeseed honey, the overall heat-clearing effect is basically the same after filtration with ethanol aqueous solution and without filtration with ethanol aqueous solution. Comparison of Comparative Example 5, Example 2 to Example 6 shows that the addition of rapeseed honey filtered with ethanol aqueous solution has a better overall heat-clearing effect, among which, it has a better effect at 5-15%, but when it reaches 20%, the overall heat-clearing effect is not much different from that of rapeseed honey filtered with aqueous solution, and is even slightly reduced.

[0052] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

[0053] In addition, those skilled in the art will appreciate that, although some embodiments herein include certain features included in other embodiments but not other features, the combination of features of different embodiments is meant to be within the scope of the present application and form different embodiments. For example, in the above claims, any one of the claimed embodiments may be used in any combination. The information disclosed in this background technology section is intended only to deepen the understanding of the overall background technology of the present application and should not be regarded as an admission or in any form of implication that the information constitutes prior art known to those skilled in the art.

Claims

1. A method for preparing a flavor-correcting Wanying granule, characterized in that: The following steps are involved: S1. The picrorhizon, coptis root, catechu, fragrant ink are crushed and stirred to form a first mixed powder; S2. The in vitro cultured bezoar and the first mixed powder in S1 were stirred and mixed to obtain a second mixed powder; S3. Add water to the bear bile powder and boil to dissolve the bear bile powder to obtain a bear bile powder liquid; S4. Concentrating and filtering the ox bile to obtain ox bile paste; S5. Add water to the ox bile paste and the bear bile powder and mix to obtain a third mixture; S6. The rapeseed nectar filtrate is added to the second mixed powder and stirred and mixed, and then the third mixture is added, and then 80~95 v / v% ethanol aqueous solution is added, stirred, and then granulated by a granulator, dried, and granulated; S7. Grind the borneol and artificial musk and sieve them and mix them with the sieved particles in S6; Among them, 50-65 weight parts of coptis root, 50-65 weight parts of picrorhizome, 50-65 weight parts of catechu, 105-125 weight parts of fragrant ink, 3-4 weight parts of borneol, 10.2-12 weight parts of bear bile powder, 2-3.5 weight parts of artificial musk, 2-3.5 weight parts of in vitro cultured bezoar, and 80-98 weight parts of ox bile; The rapeseed honey filtrate is a filtrate obtained by mixing 5-15 v / v% ethanol aqueous solution and rapeseed honey for extraction and filtration, wherein the 5-15 v / v% ethanol aqueous solution is 180-250 parts by weight and the rapeseed honey is 280-675 parts by weight; Herein, parts by weight are one of grams, tons, kilograms, thousands of kilograms or ten thousand kilograms.

2. The method for preparing the flavor correction of Wanying granules according to claim 1, characterized in that: In the preparation of the rapeseed nectar filtrate, the extraction temperature is 30-40° C. and the extraction time is 1-3 hours.

3. The method for preparing the flavor correction of Wanying granules according to claim 1, characterized in that: The 5-15 v / v% ethanol aqueous solution comprises 200-220 parts by weight and the rapeseed honey comprises 300-350 parts by weight.

4. The method for preparing the flavor correction of Wanying granules according to claim 3, characterized in that: The invention comprises 60 parts by weight of coptis root, 60 parts by weight of picrorhizome, 60 parts by weight of catechu, 120 parts by weight of fragrant ink, 3.6 parts by weight of borneol, 12 parts by weight of bear bile powder, 3 parts by weight of artificial musk, 3 parts by weight of in vitro cultured bezoar, 96 parts by weight of ox bile, 210 parts by weight of 5-15 v / v% ethanol aqueous solution, and 320 parts by weight of rapeseed honey.

5. The method for preparing the flavor correction of Wanying granules according to claim 4, characterized in that: The 80-95 v / v% ethanol aqueous solution is 180-250 parts by weight; in S3, the amount of water is 20-35 parts by weight; in S5, the amount of water is the amount of water evaporated during the concentration in S4.

6. The method for preparing the flavor correction of Wanying granules according to claim 1, characterized in that: The particle size of the first mixed powder is 170-190 μm, and the particle size of the in vitro cultured bezoar is 170-190 μm.

7. The method for preparing the flavor correction of Wanying granules according to claim 1, characterized in that: The drying temperature is 50-60° C. The granulation is performed by a swing granulator equipped with a 10-mesh stainless steel screen, and the sieving is performed by a swing granulator equipped with 10-mesh and 80-mesh stainless steel screens.

Images