Carbonized traditional Chinese medicine for resisting myocardial infarction
By using Salvia charcoal drugs, the problem of the lack of efficacy and adverse reactions in the treatment of acute myocardial infarction has been solved, and the effect of significantly reducing myocardial enzyme levels and improving antioxidant enzyme activities has been achieved, and the effect of protecting myocardial muscle is better.
Patent Information
- Application Number
- CN202410494140.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2023-10-31
- Filing Date
- 2024-04-24
- Publication Date
- 2025-05-02
AI Technical Summary
In the prior art, when treating acute myocardial infarction, the drug effect is not ideal and there are adverse reactions, making it difficult to effectively reduce the occurrence of myocardial infarction and improve the clinical symptoms after myocardial infarction.
The charcoal medicine prepared with Salvia miltiorrhiza is made by calcining the method of Salvia miltiorrhiza, and the efficacy of anti-myocardial infarction is improved through a specific firing process.
Salvia miltiorrhiza charcoal significantly reduced the myocardial enzyme level in the serum of rats with acute myocardial infarction, improved the antioxidant enzyme activity in myocardial tissue, significantly reduced myocardial damage, and had a good effect on protecting myocardial muscle.
Smart Images
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Abstract
Description
Technical Field
[0001] The invention relates to the medical field, and in particular to a new use of a type of traditional Chinese medicine charcoal medicine in the medical field. Background Art
[0002] Acute myocardial infarction (AMI) is myocardial ischemic injury caused by an imbalance in the blood supply and demand of myocardial tissue. Due to the occlusion of coronary arteries, local myocardial necrosis occurs and a large number of myocardial cells apoptosis. After AMI occurs, the most effective treatment to reduce acute myocardial ischemia / reperfusion injury (IRI), save living myocardium and limit the area of myocardial infarction is myocardial reperfusion through percutaneous coronary intervention (PPCI). However, the process of myocardial reperfusion itself can induce myocardial cell death and myocardial damage. This phenomenon is called "myocardial reperfusion injury". Western medicine conservative treatment includes the use of antithrombotic drugs, β-receptor blockers, lipid-lowering drugs, nitrates, calcium antagonists, and angiotensin-converting enzyme inhibitors (ACEI). However, the clinical efficacy of these drugs is not ideal and there are various adverse reactions.
[0003] Acute myocardial infarction belongs to the category of "chest pain" and "heartache" in traditional Chinese medicine. Modern research believes that the onset of AMI is related to underlying diseases such as long-term smoking, excessive obesity, dyslipidemia, diabetes and hypertension. When AMI occurs, patients will experience severe pain behind the sternum, abnormal increase in myocardial enzymes, and severe cases often develop heart failure and endanger their lives.
[0004] Therefore, there is a need to find a new alternative or complementary therapy to reduce the occurrence of myocardial infarction and improve clinical symptoms after myocardial infarction.
[0005] Danshen is the dried root and rhizome of Salviia miltiorrrhiza Bge. of the Lamiaceae family. Danshen charcoal is a charcoal medicine made from Danshen by frying or calcining. It is more hemostatic and is clinically used for avalanche band syndrome. There are no reports on its use in myocardial infarction.
[0006] The invention selects salvia miltiorrhiza as raw material, processes it into charcoal medicine, and studies its protective effect on myocardial infarction. Summary of the invention
[0007] The purpose of the present invention is to provide a class of safe and efficient Chinese medicinal charcoal medicines for preventing myocardial infarction, so that the medicines can be used alone or in combination in clinical practice or in drug development to improve the therapeutic effect.
[0008] Specifically, the present invention is implemented in the following way: the use of Danshen carbon in the preparation of an anti-myocardial infarction drug, and the process of carbon preparation includes the following steps:
[0009] Put the raw medicinal pieces into a crucible, wrap and seal the crucible mouth with tin foil, cover the crucible with a lid, put it into a muffle furnace and set it to a certain temperature for firing.
[0010] The invention provides a new use of Danshen charcoal with effective therapeutic effect. BRIEF DESCRIPTION OF THE DRAWINGS
[0011] Figure 1 .Effects of different doses of Danshen carbon on serum myocardial enzyme levels in rats with acute myocardial infarction. Figure 1 A shows the effect on LDH level. Figure 1 B shows the effect on ADT level. Figure 1 C shows the effect on CK levels, Figure 1 D shows the effect on CK-MB levels. ## P<0.01 compared with the control group, **P<0.01 and *P<0.05 compared with the model group. The horizontal axes Control, Model, H, M, and L in the figure represent the control group, model group, high, medium, and low dose groups, respectively.
[0012] Figure 2 Effects of different doses of Danshen carbon on the levels of SOD, MDA, GSH, CAT, and Na in myocardial tissue of rats with acute myocardial infarction + -K + -ATPase, Ca 2+ -Mg 2+ -Effects on ATPase activity levels. Figure 2 A. Figure 2 B. Figure 2 C. Figure 2 D. Figure 2 E and Figure 2 F represents SOD, MDA, GSH, CAT, Na + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase activity level. ## P<0.01 compared with the control group, **P<0.01 and *P<0.05 compared with the model group. The horizontal axes Control, Model, H, M, and L in the figure represent the control group, model group, high, medium, and low dose groups, respectively. DETAILED DESCRIPTION
[0013] The technical solution of the present invention will be clearly and completely described below in conjunction with specific embodiments, but the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0014] Embodiment 1: A method for preparing a Chinese medicine charcoal medicine (calcination method), comprising the following steps:
[0015] Put the Danshen slices into the crucible, cover the crucible mouth with aluminum foil, cover the lid tightly, and put it in a muffle furnace for carbonization (calcination temperature 200℃-400℃, time 0.5-2h). The obtained Chinese medicine charcoal is black inside and outside and has a uniform texture.
[0016] Example 2: Protective effect on myocardial infarction
[0017] 1. Experimental Animals
[0018] In this experiment, 40 SPF male SD rats, 9 weeks old, weighing 200±20g, were used as experimental subjects and were adaptively raised at a temperature of (24.0±1.0)℃ and a relative humidity of 55-65% for 7 days. During this period, the animals were guaranteed to have free access to water and food.
[0019] 2. Sample Preparation
[0020] Drug preparation: Take Danshen charcoal, crush it, add water and boil it 3 times, 1 hour each time, combine the filtrate, divide it into three parts, and concentrate it to the concentration of 1g / mL, 0.5g / mL and 0.25g / mL (calculated according to the amount of charcoal powder) as high, medium and low concentration samples respectively.
[0021] Preparation of modeling drugs: Isoproterenol hydrochloride is prepared with normal saline to prepare a modeling drug solution with a mass concentration of 85 mg / mL.
[0022] Preparation of anesthetic: Accurately weigh 10.0 g of chloral hydrate powder and add 100 ml of deionized water to prepare a 10% chloral hydrate solution.
[0023] 3. Drug administration, modeling and sampling of experimental animals
[0024] 40 SD rats were randomly divided into control group, model group, high, medium and low doses of Danshen Carbon, with 8 rats in each group. Each drug group was given the corresponding drug (10mL / kg) by gavage, and the control group and model group were given an equal volume of normal saline by gavage, once a day for 14 consecutive days. Two hours after the drug administration on the 13th day, rats in the model group and each drug group were subcutaneously injected with isoproterenol hydrochloride (85mg / kg) at multiple points, and the control group was subcutaneously injected with an equal volume of normal saline, once a day for 2 consecutive days.
[0025] Twelve hours after the second modeling, the rats in each group were anesthetized, and blood was collected from the abdominal aorta. The blood was centrifuged at 4°C and 3500 r / min for 10 min, and serum was collected. The activities of lactate dehydrogenase (LDH), creatine kinase (CK), aspartate aminotransferase (AST) and creatine kinase isoenzyme (CK-MB) in the serum were detected using an automatic biochemical analyzer.
[0026] The appropriate amount of myocardial tissue of each group of rats was cut, and 9 times the volume of normal saline was added according to the ratio of tissue weight (g): volume (mL) of 1:9. The tissue was further cut into pieces and ground in an ice water bath to make a 10% heart tissue homogenate. The homogenate was placed in a high-speed refrigerated centrifuge with the centrifugation condition set at 3000r / min. After centrifugation for 15min, the supernatant was taken and the SOD, MDA, CAT, GSH, and Na were determined according to the instructions of the kit. + -K + -ATPase, Ca2 + -Mg 2+ -ATPase activity level.
[0027] 4. Effects on serum myocardial enzyme levels in AMI rats
[0028] When myocardial tissue is ischemic, the myocardial cell membrane is damaged and myocardial enzymes are released into the blood. The levels of myocardial enzymes such as LDH, AST, CK, and CK-MB can basically reflect the severity of myocardial damage. Figure 1 As shown in the figure, the myocardial enzyme levels in the serum of rats in the saline blank group were maintained at normal levels, while those in the isoproterenol hydrochloride (ISO) induced model group were significantly increased, and there were significant differences among the data of each group (P < 0.01).
[0029] LDH levels are found in Figure 1 A, Compared with the model group, high and medium doses of Danshen Carbon can significantly reduce the level of LDH in the serum of AMI rats (P < 0.01), while low doses have no significant difference. Figure 1 B, compared with the model group, high, medium and low doses of Danshen Carbon can significantly reduce the serum AST level in AMI rats (P < 0.01). Figure 1 C, compared with the model group, high and medium doses of Danshen Carbon can significantly reduce the serum CK level in AMI rats (P < 0.01), and low dose can significantly reduce the CK level (P < 0.05). Figure 1 D. Compared with the model group, high dose of Danshen Carbon can significantly reduce the level of CK-MB in the serum of AMI rats (P<0.01), and medium and low doses of Danshen Carbon can significantly reduce the level of CK-MB (P<0.05).
[0030] 5. Effect of SOD, MDA, GSH, CAT, and Na in myocardial tissue of AMI rats + -K + -ATPase, Ca 2+ -Mg 2+ - Effect of ATPase activity level
[0031] Oxidative stress plays an important role in the pathogenesis of AMI. The levels of SOD, MDA, GSH, and CAT are important indicators for evaluating oxidative stress response. Figure 2 As shown in A, compared with the blank group, the SOD level in the myocardial tissue of the model group rats was significantly reduced, and the difference was statistically significant (P < 0.01). After 2 weeks of pre-administration, compared with the model group, high and medium doses of Danshen carbon can significantly increase the SOD level in the myocardial tissue of AMI rats (P < 0.01), and low doses of Danshen carbon can significantly increase the SOD level in the myocardial tissue of AMI rats (P < 0.05), to play an anti-oxidative stress role. The MDA level in the myocardial tissue of rats in each group is shown in Figure 2 As shown in B, compared with the blank group, the MDA level in the myocardial tissue of the model group rats was significantly increased (P < 0.01), indicating that high-dose ISO can cause excessive oxidative stress in the body. Pre-administration for 2 weeks can significantly reduce the MDA level in the myocardial tissue of AMI rats. High-dose Danshen carbon can significantly reduce the MDA level (P < 0.01), and medium and low doses of Danshen carbon can significantly reduce the MDA level (P < 0.05). GSH level Figure 2 As shown in Figure C, compared with the blank group, the GSH level in the myocardial tissue of the model group rats was significantly reduced, and the difference was statistically significant (P < 0.01). After 2 weeks of pre-administration, compared with the model group, high and medium doses of Danshen Carbon can significantly increase the GSH level in the myocardial tissue of AMI rats (P < 0.01), while there is no significant difference in the low dose of Danshen Carbon. Figure 2 As shown in D, compared with the blank group, the CAT level in the myocardial tissue of the model group rats was significantly reduced, and the difference was statistically significant (P < 0.01). After 2 weeks of pre-administration, compared with the model group, the high dose of Danshen carbon can significantly increase the CAT level in the myocardial tissue of AMI rats (P < 0.01), and the medium and low doses of Danshen carbon can significantly increase the CAT level in the myocardial tissue of AMI rats (P < 0.05), thereby playing an anti-oxidative stress role.
[0032] Na + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase is an important enzyme that maintains the balance of intracellular calcium ion concentration. When its content decreases, it often induces apoptosis and necrosis of myocardial cells. Therefore, it is important to evaluate the Na+ content in myocardial tissue. + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase levels are important for assessing the severity of acute myocardial infarction. Figure 2 E. Figure 2 F shows that compared with the blank group Na+ -K + -ATPase and Ca 2+ -Mg 2+ Compared with the level of Na-ATPase in the myocardial tissue of rats in the ISO-induced model group, + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase levels decreased significantly (P < 0.01), and the Na+ level in myocardial tissue of the high- and medium-dose groups increased significantly after 2 weeks of preventive administration. + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase levels.
[0033] In summary, the experimental results show that Danshen carbon has a good protective effect on acute myocardial infarction caused by IOS.
Claims
1. Use of a charcoal medicine prepared from a Lamiaceae plant in the preparation of an anti-acute myocardial infarction drug.
2. The use according to claim 1, characterized in that The charcoal medicine prepared from a plant of the Lamiaceae family includes Salvia miltiorrhiza charcoal.
3. The use according to claim 1, characterized in that Danshen charcoal can be used alone or as a main component in combination with other drugs.
4. The use according to claim 1, characterized in that The salvia miltiorrhiza charcoal and pharmaceutically acceptable auxiliary materials are prepared into a clinical preparation.
5. The use according to claim 4, characterized in that The clinical preparations include, but are not limited to, injections, oral preparations, external preparations, and various dosage forms for intracavitary administration.
6. A method for preparing Danshen carbon according to any one of claims 1 to 5, characterized in that: The charcoal medicine is prepared by a calcination method.