Application of cytokinin response factor RhARR17 in regulating germination of axillary buds of Chinese rose

Abstract

The invention relates to the technical field of biology, in particular to application of a cytokinin response factor RhARR17 in regulation of rosa chinensis axillary bud germination, and provides application of a product for inhibiting expression of an RhARR17 gene or transcriptional translation protein of the RhARR17 gene in promotion of rosa chinensis axillary bud germination. According to expression results of RhARR17 in different site buds of China rose branches, RhARR17 in axillary buds is instantly silenced, it is found that germination and growth of the axillary buds can be affected by abnormal expression of RhARR17, and growth of the axillary buds can be promoted by silencing RhARR17; on the contrary, the RhARR17 in the axillary buds is instantly overexpressed, so that the growth of the axillary buds can be inhibited.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to an application of a RhARR17 gene in regulating the germination of axillary buds of roses. Background Art

[0002] Rose (Rosa hybrida) is a perennial evergreen or semi-evergreen woody ornamental plant of the genus Rosa in the family Rosaceae. Rose has the characteristics of dignified flower shape, bright color and rich fragrance. It has high ornamental and economic value. It ranks first among the four major cut flowers in the world and is one of the most consumed flowers in the cut flower market. It is widely used in landscaping, garden decoration, potted flowers, spices and cosmetics industries.

[0003] Germination is an important biological characteristic in the growth and development of plants. The germination of axillary buds determines the formation of plant branches, and the number of branches directly affects the number of nutritional organs, which has a decisive impact on crop yield. The differentiation process of meristems forms axillary buds. First, the meristems differentiate into lateral bud primordia, which further develop into axillary buds and finally form lateral branches. The number of lateral branches in plants is closely related to the germination of axillary buds, and the two are positively correlated.

[0004] The axillary buds of roses mainly develop in the axils of leaves, and their further growth can form flowering branches. Therefore, this process is crucial to the growth and morphological formation of roses, and axillary buds are also one of the most important proliferation materials for roses. In the production process of cut roses, the speed of axillary bud germination directly affects its survival rate; and in the entire growth and development process, the characteristics of axillary buds determine the growth cycle, yield and quality of cut roses. Therefore, studying and regulating the relevant factors of axillary bud germination of roses has important practical significance for the breeding of cut roses and improving the quality and efficiency of the industry. Summary of the invention

[0005] The present invention screened out the cytokinin corresponding factor RhARR17, and found that the gene was highly expressed in the axillary buds and sepals of rose tissues, affecting the germination of the axillary buds of roses, and inhibiting the expression of the gene could promote the germination of the axillary buds of roses.

[0006] To achieve the above objectives, the present invention provides the following technical solutions: In one aspect, the present invention provides an application of inhibiting the expression of RhARR17 gene or its transcriptional translation protein in promoting the germination of axillary buds of rose. The base sequence of the RhARR17 gene is shown in SEQ ID NO.1.

[0007] On the other hand, the present invention provides an application of a product for inhibiting the expression of RhARR17 gene or its transcriptional translation protein in promoting the germination of axillary buds of roses, wherein the base sequence of the RhARR17 gene is shown in SEQ ID NO.1.

[0008] Furthermore, the product is a product for silencing, knocking out or knocking down the RhARR17 gene.

[0009] Furthermore, the product of silencing the RhARR17 gene contains the base sequence described in SEQ ID NO.2.

[0010] On the other hand, the present invention provides a kit for use in promoting the germination of axillary buds of roses, wherein the kit contains a reagent for inhibiting the expression of the RhARR17 gene, and the base sequence of the RhARR17 gene is shown in SEQ ID NO.1.

[0011] On the other hand, the present invention provides an isolated polynucleotide, the base sequence of which is shown in SEQ ID NO.2. The polynucleotide is used to inhibit the expression of the RhARR17 gene or its transcriptional translation protein, and the base sequence of the RhARR17 gene is shown in SEQ ID NO.1.

[0012] In another aspect, the present invention provides a vector comprising the base sequence shown in SEQ ID NO: 2.

[0013] In another aspect, the present invention provides an engineered bacterium, wherein the engineered bacterium contains the vector.

[0014] Furthermore, the present invention provides the use of the polynucleotide, or the vector, or the engineered bacteria in promoting the germination of axillary buds of roses.

[0015] On the other hand, the present invention provides a method for promoting the germination of axillary buds of roses, the method comprising inhibiting the expression of the RhARR17 gene or its transcriptional translation protein in roses, the base sequence of the RhARR17 gene being shown in SEQ ID NO.1.

[0016] Beneficial effects of the present invention: The present invention identified a cytokinin corresponding factor RhARR17 from the rose genome, and found that the gene was highly expressed in the axillary buds and sepals of rose tissues, and the expression signal was concentrated in the axillary buds, and almost no expression was found in the petals and stem segments. In the analysis of the expression of RhARR17 in buds at different locations of rose branches, it was found that the expression of RhARR17 was high in the axillary buds at the middle position. Therefore, the present application speculates that RhARR17 may be involved in the germination of axillary buds of roses.

[0017] In the present invention, based on the expression results of RhARR17 in buds at different positions of rose branches, RhARR17 in axillary buds was transiently silenced, and it was found that abnormal expression of RhARR17 would affect the germination and growth of axillary buds, and silencing RhARR17 could promote the growth of axillary buds; RhARR17 in axillary buds was transiently overexpressed, and overexpression of RhARR17 could inhibit the growth of axillary buds. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is the expression analysis diagram of RhARR17 provided by the present invention; Note: they are stem segment, root, petal, leaf, sepal, axillary bud, stamen and pistil respectively.

[0019] Figure 2 It is a subcellular localization diagram of RhARR17 provided by the present invention; Note: (A) RhARR17 subcellular localization prediction result; (B) Subcellular localization in Nicotiana benthamiana leaves, RhARR17-GFP and NF-YA4-mCherry (nuclear marker) were co-infiltrated into tobacco leaves, and the fluorescence signal was observed by confocal microscopy.

[0020] Figure 3 This is a statistical chart of quantitative analysis of buds at different nodes of RhARR17; the data are shown as mean ± SD (n = 3) (**** indicates P ≤ 0.0001), which are upper axillary buds, middle axillary buds, and lower axillary buds, respectively.

[0021] Figure 4 It is a phenotypic observation result diagram of axillary bud germination and growth at different time periods after transient silencing of RhARR17 provided by the present invention; Note: 0d, 4d, 8d, represent the growth of TRV2 and TRV2-RhARR17 silenced axillary buds on the 0th, 4th, and 8th days after infection.

[0022] Figure 5 It is an anatomical diagram of the growth status of axillary buds in different time periods after transient silencing of RhARR17 provided by the present invention; Note: 0d, 4d, and 8d represent the anatomical observation of the growth points of the TRV2 and TRV2-RhARR17 silenced axillary buds on the 0th, 4th, and 8th days after infection.

[0023] Figure 6 It is a statistical graph of gene expression and axillary bud length measurement after transient silencing of RhARR17 provided by the present invention; Note: (A) RT-qPCR analysis of RhARR17 relative expression after silencing; (B) Bud length of the control group TRV2 and the treatment group TRV2-RhARR17 silenced 0d, 4d, and 8d after silencing (* indicates P≤0.05, ** indicates P≤0.01).

[0024] Figure 7It is a phenotypic observation result diagram of axillary bud germination and growth at different time periods after transient overexpression of RhARR17 provided by the present invention; Note: 0d, 6d, and 12d represent the growth of axillary buds overexpressing pSuper-1300 and pSuper-1300-RhARR17 on days 0, 6, and 12 after infection.

[0025] Figure 8 It is an anatomical diagram of the growth status of axillary buds at different time periods after transient overexpression of RhARR17 provided by the present invention; Note: 0d, 6d, and 12d represent the anatomical observation of the growth points of the axillary buds overexpressing Super-1300 and pSuper-1300-RhARR17 on days 0, 6, and 12 after infection.

[0026] Fig. 9 It is a statistical graph of gene expression and axillary bud length measurement after transient overexpression of RhARR17 provided by the present invention; Note: (A) RT-qPCR analysis of RhARR17 relative expression after overexpression; (B) Bud length of the control group pSuper-1300 and the treatment group pSuper-1300-RhARR17 overexpression at 0d, 6d, and 12d after overexpression (* indicates P≤0.05, ** indicates P≤0.01). DETAILED DESCRIPTION

[0027] The following is a detailed description in conjunction with the embodiments and drawings. The present application performs transcriptome sequencing on axillary buds at different time points after pruning, and screens out the cytokinin corresponding factor RhARR17 with significant differences related to the germination of axillary buds of roses. It is highly expressed in axillary buds, and it is found that the expression level of RhARR17 is related to the activity of axillary buds, with the highest expression in the upper axillary buds, followed by the middle axillary buds, and the lowest expression in the lower axillary buds. After transient silencing and transient overexpression of RhARR17, it was found that axillary bud germination and growth rate were affected. Therefore, the present application reveals the biological function of RhARR17 in the germination of axillary buds of roses, which is of great significance to the breeding of cut roses and the improvement of quality and efficiency of the industry.

[0028] The present invention provides a base sequence of the RhARR17 gene as shown in SEQ ID NO: 1, and a base sequence of the silencing RhARR17 gene as shown in SEQ ID NO: 2.

[0029] The abbreviations and their corresponding names appearing in this application are shown in Table 1.

[0030] Table 1 Abbreviations Example

[0031] 1. Plant materials 1.1 'Pink Snow Mountain' 'Pink Snow Mountain' is a modern cut rose variety, taken from the Baofeng Base of the Institute of Flowers and Plants of Yunnan Academy of Agricultural Sciences. The stem segments with single buds were used as materials for the rose axillary bud germination experiment.

[0032] 1.2 Tobacco The experimental material for subcellular localization was Nicotiana benthamiana. The seeds were sown in a moist nutrient matrix, covered with a film, and cultured in a culture room.

[0033] Culture conditions: temperature 24±1℃, relative humidity 60-65%, photoperiod 16h / 8h.

[0034] 1.3 Strains and vectors Escherichia coli: DH5α and Agrobacterium tumefaciens strains: EHA105 and pSuper-1300 (Kan resistance) were purchased from Beijing Qingke Biotechnology Co., Ltd. VIGS vectors were pTRV1 and pTRV2 purchased from HonorGene.

[0035] 1.4 Culture medium formula involved in the case (1) LB medium and YEB medium (Table 2) Table 2 LB and YEB medium formula

[0036] 2. Research Methods 2.1 Total RNA extraction Total RNA was extracted from the petals, leaves, receptacles, sepals and axillary buds of 'Pink Snow Mountain' using RNAprep Pure Polysaccharide and Polyphenol Plant Total RNA Extraction Kit (centrifugal column type).

[0037] 2.2 cDNA synthesis (1) Genomic DNA removal (Table 3) Table 3 Genomic DNA removal reaction system

[0038] Mix well and incubate at 42°C for 2 min.

[0039] (2) Prepare the reverse transcription reaction system (Table 4) Table 4 Transcription reaction system

[0040] Mix by pipetting, incubate at 37°C for 15 min, and 85°C for 5 s. Store the product at -20°C.

[0041] 2.3 Real-time fluorescence quantitative PCR Primer premier5 was used to design gene-specific primers (Table 5). The cDNA was diluted four times with ddH2O, and the cDNA obtained by reverse transcription was used as a template for Real-Time PCR amplification. GAPDH was used as an internal reference, and three biological replicates were set.

[0042] Table 5 RT-qPCR primer sequences

[0043] The reaction system is shown in Table 6: Table 6 RT-qPCR reaction system

[0044] The reaction procedure is shown in Table 7: Table 7 RT-qPCR reaction procedure

[0045] 2.4 Vector construction 2.4.1 PCR amplification of target gene fragments High-fidelity enzyme (PhusionTM Plus PCR Master Mix) was used for PCR target gene amplification, and the reaction system is shown in Table 8: Table 8 PCR amplification reaction system

[0046] The reaction procedure is shown in Table 9: Table 9 PCR amplification reaction program

[0047] After amplification, 1% gel electrophoresis was performed and the target band was selected for subsequent gel recovery.

[0048] 2.4.2 Rubber Recycling The PCR product was recovered and purified using a DNA gel recovery kit (TaKaRa MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0).

[0049] 2.4.3 Double restriction digestion of vector According to the restriction site where the target fragment sequence is inserted, the vector is double-digested with the corresponding enzyme. The restriction system is shown in Table 10: Table 10 Double enzyme digestion system

[0050] After adding the system reagents on ice, perform double enzyme digestion of the vector according to the thermal denaturation temperature of the specific enzyme.

[0051] 2.4.4 Homologous recombination The double-digested vector and the cloned target gene fragment are homologously recombined to construct the vector. The homologous recombination system is shown in Table 11: Table 11 Homologous recombination system

[0052] Procedure: Run in PCR instrument at 50°C for 15 min.

[0053] 2.4.5 E. coli transformation (1) Take out the competent cells from -80 °C and thaw them in ice; (2) Take a 1.5 mL centrifuge tube, add 10 μL of the recombinant product and 50 μL of DHα competent cell, mix well by pipetting, and place on ice for 30 min; (3) Heat shock in a 42°C water bath for 90 s, then quickly transfer to ice and let stand for 2 min; (4) Add 500 μL LB to the centrifuge tube and incubate at 37 °C, 200 rpm, for 1 h. (5) Centrifuge the cultured bacterial solution at 5000 rpm for 5 min; (6) Discard 400 μL of supernatant in a clean bench, mix thoroughly by pipetting, and then spread on LB solid medium containing antibiotics, and culture inverted at 37°C overnight.

[0054] 2.4.6 Bacterial testing and sequencing (1) Shaking: Pick a single colony grown from the overnight culture and culture it in 500 μL of LB medium containing antibiotics with shaking for 3-4 h (37 ℃, 200 rpm).

[0055] (2) Bacterial liquid PCR: The cultured bacterial liquid is used for PCR amplification to detect whether the target band of the constructed vector meets the expectations. The bacterial liquid PCR amplification system is shown in Table 12: Table 12 Bacterial liquid PCR amplification system

[0056] The bacterial solution PCR amplification program is shown in Table 13: Table 13 Bacterial liquid PCR amplification reaction program

[0057] The amplified products were used for 1% gel electrophoresis imaging, and those with expected band sizes were sent to the company for sequencing.

[0058] 2.5 Plasmid extraction After the test results are returned, sequence comparison is performed, and the samples that meet the expectations are selected for bacterial culture, and the plasmid is extracted using a plasmid extraction kit (TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0).

[0059] 2.6 Agrobacterium transformation (1) Take out the competent stem from the -80 ℃ freezer and put it into ice when it melts to an ice-water mixture; (2) Add 0.01-1 μg of plasmid DNA per 100 μL competent medium, mix thoroughly, and place on ice for 5 min, in liquid nitrogen for 5 min, in a 37°C water bath for 5 min, and in an ice bath for 5 min. (3) Add 500 μL of YEB liquid medium without antibiotics and culture at 28 °C with shaking for 2-3 h; (4) Centrifuge at 5000 rpm for 2 min. Discard 400 μL of supernatant on a clean bench and spread onto YEB solid medium containing antibiotics. Incubate inverted at 28 °C for 2-3 d.

[0060] 2.7 Subcellular localization (1) Vector construction SmaI and KpnI were selected as restriction sites, and the complete coding region (SEQ ID NO: 1) sequence of RhARR17 was inserted into the pSuper-1300 vector. Primers were designed using homologous recombination to construct the RhARR17-GFP vector. After obtaining Agrobacterium-positive strains, the following experiments were performed.

[0061] (2) Shaking bacteria 1) Streak culture of bacterial liquid: Take out the stored bacterial liquid and streak it on YEB medium containing corresponding antibiotics, and culture it upside down at 28℃ for 2-3 days; 2) Shaking: Pick a single colony into a 1.5 mL centrifuge tube, add 500 μL of YEB liquid culture medium containing antibiotics and shake overnight (28 ℃, 200 rpm), perform PCR bacterial test, and continue to shake the bacterial solution with the correct band at medium and high speed.

[0062] (3) Tobacco injection 1) Collection of bacteria: Culture to OD 600 =0.4-0.6, centrifuge (5000 rpm, 8 min), discard the supernatant, and collect the bacteria. Resuspend the bacteria in the infection solution and adjust the OD 600 Adjust to 1.0, mix at a ratio of RhARR17-GFP / GFP: NF-YA4-mCherry: P19 = 1:1:0.5, and incubate in the dark for 3 h.

[0063] 2) Injection: After the rest period, use a needle to gently make a cut on the back of the tobacco (when it has grown to 4-6 leaves), and use a 1 mL syringe to inject the mixed bacterial solution into the tobacco leaves in the right proportion, and mark them; 3) Cultivation: Place the injected tobacco in a 25 ℃ incubator, incubate in the dark for one day, and then transfer to light for 2 days; pay attention to watering during this period to maintain normal growth of the tobacco.

[0064] (4) Observation and photography Select the tobacco leaves near the injection port, cut them into squares with a blade and tweezers, place them on a glass slide, drip distilled water on them, and cover them with a coverslip (be careful to avoid bubbles). Use a laser confocal microscope from the State Key Laboratory of the Institute of Biology, Yunnan Academy of Agricultural Sciences to observe and take pictures.

[0065] 2.8 Momentary Silence (1) Vector primer design and vector construction Using EcoRI and BamHI as restriction sites, the silencing fragment of RhARR17 (SEQ ID NO: 2) was inserted into the TRV2 empty vector, and primers were designed using homologous recombination to construct the TRV-RhARR17 vector.

[0066] (2) Bacterial culture Streak the bacterial solution plate (containing Kan / Rif 50 mg / L) and invert and culture at 28 ℃ for 2-3 days. Pick a single colony and shake it in 500 μL YEB containing antibiotics for bacterial inspection; shake it medium and high (28 ℃, 200 rpm) if the strip is correct.

[0067] (3) Collection and resuspension Centrifuge at 5000 rpm for 8 min to collect the bacteria, discard the supernatant, resuspend the bacteria in the infection solution, mix with a pipette, and adjust to OD600 = 1.0. When performing transient silencing experiments, mix TRV1 and TRV2, and TRV2-RhARR17 bacterial solutions in a volume ratio of 1:1 and let stand in the dark for 4-6 h.

[0068] (4) Vacuum suction Use a vacuum pump to infect the stem segments with single buds, 0.082 MPa, suction for 10 min, hold pressure for 10 min, release air for 10 min, and immerse the entire stem segment in the bacterial solution as much as possible. Repeat the process three times. After infection, rinse with sterile water, place in a culture room at 8℃ for 3 days, and then cut the cuttings. Observe every two days, take samples, and take photos for record.

[0069] 2.10 Primers used in the RhARR17 sequence experiment Table 14 RhARR17 sequence and primer list

[0070] 3 Test results 3.1 Verification of RhARR17 expression in different tissues In this application, total RNA from rose petals, leaves, receptacles, sepals, and axillary buds was extracted and quality tested. Genes with the most cis-acting elements related to meristem and plant growth and development were selected for fluorescence quantitative expression analysis. It was found that different genes were expressed differently in different tissues ( Figure 1 Among them, RhARR17 was expressed more specifically and at higher levels in axillary buds and sepals, indicating that RhARR17 may be essential for the growth and development of rose axillary buds.

[0071] 3.2 Subcellular localization analysis of RhARR17 In order to explore the subcellular location of RhARR17 function, this application first used Cell-PLoc (http: / / www.csbio.sjtu.edu.cn / bioinf / Cell-PLoc-2) for online prediction, which showed that it was localized in the cell nucleus ( Figure 2 A). To further verify the accuracy of localization, the CDS sequence of RhARR17 was cloned and the RhARR17-GFP vector was constructed. Using GFP empty vector as control, it was transferred into Agrogan EH105 and then injected into Nicotiana benthamiana leaves. After 3 days, the leaves were observed under a laser confocal microscope. The results showed that both GFP empty vector and RhARR17-GFP were localized in the cell nucleus ( Figure 2 B).

[0072] 3.4 Analysis of RhARR17 expression in different active buds In this application, the expression level of RhARR17 in axillary buds at different nodes of the cut rose 'Pink Snow Mountain' was analyzed by real-time fluorescence quantitative PCR. It was found that the expression of RhARR17 was higher in the upper axillary buds, followed by the middle axillary buds, and the lowest in the lower axillary buds ( Figure 3 ). Therefore, the present application speculates that RhARR17 is closely related to the germination of axillary buds.

[0073] 3.5 RhARR17 inhibits rose axillary bud germination Based on the expression analysis results of RhARR17 in buds at different sites, the specific segment of RhARR17 sequence (SEQ ID NO: 2) was selected, and the TRV-RhARR17 transient silencing vector was constructed by homologous recombination. TRV1, TRV2 and TRV2-RhARR17 were transferred into Agrocanthus EHA105 and used to infect the middle axillary bud stem segments to silence RhARR17. Phenotypic observations and records were performed at 0 d, 4 d and 8 d after infection ( Figure 4 ). From the bud phenotype, compared with the TRV2 control, the bud germination speed of TRV2-RhARR17 was faster. The dissection results of the buds also showed that the development of the buds was accelerated after RhARR17 was silenced ( Figure 5 ). In order to confirm the silencing effect, total RNA of buds before and after RhARR17 silencing was extracted, and its expression level was analyzed by RT-qPCR. It was found that the expression of RhARR17 was significantly reduced after silencing, indicating that the silencing was effective ( Figure 6 A). In addition, the bud lengths of the control group TRV2 and the experimental group TRV2-RhARR17 at different time points were measured, and it was found that the difference was extremely significant on the 10th day after silencing ( Figure 6 B).

[0074] Based on the expression analysis results of RhARR17 in buds at different sites, the CDS sequence of RhARR17 (SEQ ID NO: 1) was selected, and the pSuper-1300-RhARR17 transient overexpression vector was constructed by homologous recombination. pSuper-1300 and pSuper-1300-RhARR17 were transferred into Agrogan EHA105 and used to infect the middle axillary bud stem segments to overexpress RhARR17. Phenotypic observations and records were performed at 0 d, 6 d, and 12 d after infection ( Figure 7 ). From the phenotype of the buds, compared with the pSuper-1300 control, the germination speed of the pSuper-1300-RhARR17 buds is slower. The dissection results of the buds also show that the development of the buds is slowed down after RhARR17 overexpression ( Figure 8 ). In order to confirm the overexpression effect, total RNA of buds before and after RhARR17 overexpression was extracted, and its expression level was analyzed by RT-qPCR. It was found that the expression of RhARR17 increased significantly after overexpression, indicating that the overexpression was effective ( Fig. 9 A). In addition, the bud lengths of the control group pSuper-1300 and the experimental group pSuper-1300-RhARR17 at different time points were measured, and it was found that the difference was extremely significant at 12 days after silencing ( Fig. 9 B).

[0075] It should be noted that the above specific embodiments are exemplary, and those skilled in the art can come up with various solutions inspired by the disclosure of the present invention, and these solutions also belong to the disclosure scope of the present invention and fall within the protection scope of the present invention. Those skilled in the art should understand that the present invention description and its drawings are illustrative and do not constitute a limitation of the claims. The protection scope of the present invention is defined by the claims and their equivalents.

Claims

1. Application of inhibiting the expression of RhARR17 gene or its transcriptional translation protein in promoting the germination of axillary buds of rose, characterized in that: The base sequence of the RhARR17 gene is shown in SEQ ID NO.

1.

2. The use of a product for inhibiting the expression of the RhARR17 gene or its transcriptional translation protein in promoting the germination of axillary buds of roses, characterized in that: The base sequence of the RhARR17 gene is shown in SEQ ID NO.

1.

3. The use according to claim 2, characterized in that: The product is a product for silencing, knocking out or knocking down the RhARR17 gene.

4. The use according to claim 3, characterized in that: The product for silencing the RhARR17 gene contains the base sequence described in SEQ ID NO.

2.

5. Use of a kit in promoting the germination of axillary buds of roses, characterized in that: The kit contains a reagent for inhibiting the expression of the RhARR17 gene, and the base sequence of the RhARR17 gene is shown in SEQ ID NO.

1.

6. An isolated polynucleotide, characterized in that The base sequence of the polynucleotide is shown as SEQ ID NO.

2. The polynucleotide is used to inhibit the expression of the RhARR17 gene or its transcriptional translation protein. The base sequence of the RhARR17 gene is shown as SEQ ID NO.

1.

7. A carrier, characterized in that The vector contains the base sequence shown in SEQ ID NO:

2.

8. An engineered bacterium, characterized in that: The engineered bacteria contains the vector according to claim 7.

9. Use of the polynucleotide according to claim 6, or the vector according to claim 7, or the engineered bacteria according to claim 8 in promoting the germination of axillary buds of roses.

10. A method for promoting the germination of axillary buds of roses, characterized in that: The expression of the RhARR17 gene or its transcriptional translation protein in rose is inhibited, and the base sequence of the RhARR17 gene is shown in SEQ ID NO.1.

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