Preparation process of arginine for improving skin moisture retention and application of arginine cosmetics

Abstract

The invention belongs to the technical field of chemical processes, and discloses a preparation process of arginine for improving skin moisture retention and application of cosmetics thereof, and the preparation process comprises the following steps: 1, adding common carbohydrates such as glucose and sucrose into a culture medium, with the content of glucose in the culture medium being 5-15% (mass fraction), the content of sucrose in the culture medium being 5-15% (mass fraction), and the content of arginine in the culture medium being 5-15% (mass fraction); the addition amount of ammonia water can be properly added according to the pH value regulation and control requirement in the fermentation process, and the content of ammonium sulfate can be about 1%-3%; inorganic salts including monopotassium phosphate (0.1%-0.3%), magnesium sulfate (0.05%-0.1%) and the like are added, and a proper amount of growth factors such as biotin (the general concentration is 5-20 [mu] g / L) and the like are added; the material supplementing pipe is disinfected through the disinfection box and the telescopic pipe, and disinfectant is collected through the collection block, so that the material supplementing pipe can be disinfected before or after material supplementing, thorough sterilization treatment is carried out, bacteria, molds and other microorganisms do not remain in the material supplementing pipe, and the microorganisms are prevented from entering the material supplementing pipe along with the supplemented material; therefore, the yield and quality of arginine can be improved.

Description

Technical Field

[0001] The invention belongs to the technical field of chemical process, and in particular relates to an arginine preparation process for improving skin moisturizing ability and application of the same in cosmetics. Background Art

[0002] Arginine is a commonly used nutritional additive in cosmetics. It is mainly used for conditioning dry skin in cosmetics. When used in conjunction with trehalose, which has a moisturizing effect, it can retain skin moisture, smooth the skin, and reduce dandruff. The arginine used to improve skin moisturizing ability is usually L-arginine. Adding L-arginine to cosmetics during the production of cosmetics can make the cosmetics have a certain effect of improving skin moisturizing ability.

[0003] However, in the prior art, L-arginine is prepared by microbial fermentation, and during fermentation, feed needs to be added to the inside through a feed tube. If the feed tube is not thoroughly sterilized before or after use, bacteria, molds and other microorganisms will remain in the tube. These microorganisms enter with the feed, compete with the arginine-producing bacteria for nutrients and living space, and affect the yield and quality of arginine. Summary of the invention

[0004] The present invention aims at solving the problems in the prior art and proposes the following technical solution: a process for preparing arginine for improving the moisturizing ability of skin, the steps of which include: Step 1: Add commonly used glucose, sucrose and other sugar substances to the culture medium. The content of glucose in the culture medium is 5% to 15% (mass fraction). The amount of ammonia water added can be added in appropriate amounts according to the pH value control requirements during the fermentation process. The content of ammonium sulfate can be around 1% to 3%. Add inorganic salts: including potassium dihydrogen phosphate (0.1% to 0.3%), magnesium sulfate (0.05% to 0.1%), etc., and add appropriate amounts of biotin (generally at a concentration of 5-20μg / L) and other growth factors; Step 2: First, inoculate the strain into a seed shake bottle or seed tank filled with seed culture medium, and culture it at a suitable temperature and speed for 12-24 hours to obtain a seed solution with vigorous growth and good vitality; Step 3: Add the seed liquid to the fermentation tank in a certain proportion (usually 5%-15% volume ratio), maintain the pH value between 6.5-7.5 by automatically adding ammonia water, and keep the dissolved oxygen content at 20%-30% saturation. The fermentation time lasts about 48-72 hours, and control the temperature, pH value, dissolved oxygen content and other parameters; Step 4: After the fermentation is completed, remove impurities such as bacteria by filtering or centrifugation to obtain a fermentation broth containing L-arginine. Then, use a strong acid cation exchange resin to adsorb L-arginine on the fermentation broth, then elute it with a suitable eluent, and then obtain purer L-arginine through concentration and crystallization.

[0005] As a preferred embodiment of the above technical solution, it includes a fermentation tank, a tank cover is arranged on the top of the fermentation tank, a feeding pipe is arranged on one side of the top of the tank cover, a stirring motor is arranged in the middle of the top of the tank cover, a connecting rod is connected to the output end of the stirring motor for transmission, a stirring rod is fixedly installed at one end of the connecting rod, a stirring block is fixedly installed on the outside of the stirring rod, feeding pipes are arranged on the left and right sides of the connecting rod, a sealing cover is arranged on the top of the feeding pipe, one end of the feeding pipe is fixedly installed with a mounting block through the tank cover, two movable grooves are arranged inside the mounting block, a feeding port is arranged at the bottom of the two movable grooves and at the bottom of the mounting block, a first electric push rod is arranged inside the two movable grooves, a collecting block is fixedly installed on one side of the first electric push rod, a mounting groove is arranged at the bottom of the tank cover, and a sealing cover is arranged at the top of the feeding pipe. Two mounting plates are arranged at the bottom, and a disinfection box is arranged on the top of the two mounting plates. Disinfectant is arranged inside the disinfection box. One side of the disinfection box is fixedly installed with the inner wall of the mounting groove, a telescopic tube is fixedly installed on one side of the disinfection box, one end of the telescopic tube is rotatably connected with a nozzle, a control valve is fixedly installed on the end of the telescopic tube close to the disinfection box, and a movable structure is arranged on one side of the disinfection box and the telescopic tube and inside the mounting groove. A groove is provided on the side of the mounting groove away from the disinfection box, a connecting port is provided in the middle of the groove, and the connecting port runs through one side of the feeding pipe, and sealing blocks are slidably connected to the inner walls of the upper and lower sides of the groove, the sealing blocks are arranged corresponding to the connecting port, a second electric push rod is fixedly installed on one side of the sealing block, and one side of the second electric push rod is fixedly installed on the inner wall of the groove away from the sealing block.

[0006] As a preferred embodiment of the above technical solution, a support frame is arranged on the outside of the connecting rod, the bottom of the support frame is fixedly mounted on the top of the tank cover, the bottom of the stirring motor is fixedly mounted on the top of the support frame, the feeding pipe is located on both sides of the support frame, the connecting rod and the stirring rod extend through the tank cover and the mounting block to the interior of the fermentation tank, the mounting block is located at the top of the interior of the fermentation tank, and it is mounted on the inner wall of the fermentation tank.

[0007] As a preferred embodiment of the above technical solution, the feeding port is located on both sides of the stirring rod, a fixed plate is inserted into one side of the first electric push rod, the fixed plate is fixedly installed on the inner wall of the movable groove, the collecting block is located on one side of the feeding port, and a movable port is opened on the side of the mounting block close to the collecting block, a protective door is hinged at the movable port, and the fixed plate, the first electric push rod, the collecting block and the protective door are all symmetrically arranged around the center of the mounting block.

[0008] As a preferred embodiment of the above technical solution, a sealing plate is hinged at the bottom of the mounting block and at the bottom of the feeding port, a connecting spring is fixedly installed on the top of one side of the sealing plate, a fixing hole is opened at the bottom of the mounting block and at one side of the feeding port, and one end of the connecting spring is fixedly installed on the inner wall of the fixing hole.

[0009] As a preferred embodiment of the above technical solution, the moving structure includes a servo motor, the output end of the servo motor is transmission-connected with a rotating rod, one end of the rotating rod is fixedly mounted with a gear, both sides of the gear are respectively meshed with a first rack and a second rack, one side of the first rack and the second rack is fixedly mounted with a connecting plate, and one end of the connecting plate is fixedly mounted with the telescopic tube.

[0010] As a preferred embodiment of the above technical solution, the shape of the mounting groove is U-shaped, the top of the servo motor is fixedly installed on the inner wall of the top of the mounting groove, the bottom of the rotating rod is rotatably connected to the inner wall of the bottom of the mounting groove, one side of the first rack and the second rack are both slidably connected to the inner wall of the movable groove, a movable rod is fixedly installed on the top of the connecting plate, and the top of the movable rod is slidably connected to the inner wall of the top of the mounting groove, and the first rack, the second rack, the connecting plate, the movable rod, the disinfection box and the telescopic tube are symmetrically arranged with the center of the tank cover.

[0011] As a preferred embodiment of the above technical solution, a sealing ring is fixedly installed on the outside of one end of the feeding pipe close to the mounting block, a clamping plate is arranged on the outside of the sealing ring, one side of the clamping plate is arc-shaped, and the arc-shaped side of the clamping plate is in contact with the outside of the sealing ring, an electric telescopic rod is fixedly installed on one side of the clamping plate, a fixed block is fixedly installed on one side of the electric telescopic rod, the bottom of the fixed block is fixedly installed on the top of the mounting block, and the fixed block, the electric telescopic rod and the clamping plate are distributed in a circular array with the center of the sealing ring.

[0012] The invention also discloses an application of an arginine preparation process for improving skin moisturizing ability, which is applied to emulsions, water, creams, essences, facial masks, soaps, shampoos, conditioners, shower gels, cleansers, etc. in cosmetics.

[0013] The beneficial effects of the present invention are: (1) The present invention disinfects the feeding pipe by using a disinfection box and a telescopic tube, and collects the disinfectant by using a collecting block, so that the feeding pipe can be disinfected before or after feeding, and a thorough sterilization treatment is performed, so that no bacteria, molds and other microorganisms remain in the feeding pipe, and these microorganisms are prevented from entering with the feeding, thereby facilitating the improvement of the yield and quality of arginine; (2) The present invention uses the electric telescopic rod and the tightening block to tighten the sealing ring, thereby improving the sealing performance of the sealing ring, so that the connection between one end of the feeding tube and the mounting block remains sealed, preventing the connection between the feeding tube and the mounting block from being loosely sealed, resulting in microorganisms in the outside air possibly entering the feeding tube and further contaminating the feeding tube. At the same time, by rotating the protective door, the disinfectant inside the collection block can be easily taken out for replacement, and the disinfectant can be easily collected again, thereby facilitating use. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 What is shown is a schematic diagram of the overall structure of the embodiment; Figure 2 Shown is a cross-sectional view of an embodiment; Figure 3 The embodiment shown is Figure 2 A magnified image of point A; Figure 4 Shown is a structural diagram of a tank cover, a mounting block and a stirring rod of an embodiment; Figure 5 What is shown is the structural diagram of the mounting block and the feeding pipe of the embodiment; Figure 6 Shown is a structural diagram of the mounting block, feed pipe and sealing plate of the embodiment; Figure 7 Shown is a structural diagram of an embodiment of a can lid; Figure 8 Shown is a diagram of the internal structure of the tank cover of an embodiment; Fig. 9 Shown is a diagram of the internal structure of the mounting slot of an embodiment.

[0015] In the figure: 1. fermentation tank; 2. tank cover; 3. feed pipe; 4. stirring motor; 5. connecting rod; 6. stirring rod; 7. feeding pipe; 8. sealing cover; 9. mounting block; 10. first electric push rod; 11. collecting block; 12. mounting plate; 13. disinfection box; 14. telescopic tube; 15. moving structure; 151. servo motor; 152. rotating rod; 153. gear; 153. first rack; 154. second rack; 155. connecting plate; 16. sealing block; 17. second electric push rod; 18. supporting frame; 19. protective door; 20. sealing plate; 21. connecting spring; 22. moving rod; 23. sealing ring; 24. pressing plate; 25. electric telescopic rod; 26. fixing block. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical solution and advantages of the embodiments of the present invention more clear, the technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments.

[0017] The present invention provides a process for preparing arginine for improving skin moisturizing ability, the steps of which include: Step 1: Add commonly used glucose, sucrose and other sugar substances to the culture medium. The content of glucose in the culture medium is 5% to 15% (mass fraction). The amount of ammonia water added can be added in appropriate amounts according to the pH value control requirements during the fermentation process. The content of ammonium sulfate can be around 1% to 3%. Add inorganic salts: including potassium dihydrogen phosphate (0.1% to 0.3%), magnesium sulfate (0.05% to 0.1%), etc., and add appropriate amounts of biotin (generally at a concentration of 5-20μg / L) and other growth factors; Step 2: First, inoculate the strain into a seed shake bottle or seed tank filled with seed culture medium, and culture it at a suitable temperature and speed for 12-24 hours to obtain a seed solution with vigorous growth and good vitality; Step 3: Add the seed liquid into the fermentation tank 1 in a certain proportion (usually 5%-15% by volume), maintain the pH value between 6.5-7.5 by automatically adding ammonia water, etc., and keep the dissolved oxygen content at 20%-30% saturation. The fermentation time lasts about 48-72 hours, and control the temperature, pH value, dissolved oxygen content and other parameters; Step 4: After the fermentation is completed, remove impurities such as bacteria by filtering or centrifugation to obtain a fermentation broth containing L-arginine. Then, use a strong acid cation exchange resin to adsorb L-arginine on the fermentation broth, then elute it with a suitable eluent, and then obtain purer L-arginine through concentration and crystallization.

[0018] like Figures 1 to 5As shown, it includes a fermentation tank 1, a tank cover 2 is arranged on the top of the fermentation tank 1, a feeding pipe 3 is arranged on one side of the top of the tank cover 2, a stirring motor 4 is arranged in the middle of the top of the tank cover 2, a connecting rod 5 is connected to the output end of the stirring motor 4, a stirring rod 6 is fixedly installed at one end of the connecting rod 5, a stirring block is fixedly installed on the outside of the stirring rod 6, feeding pipes 7 are arranged on the left and right sides of the connecting rod 5, a sealing cover 8 is arranged on the top of the feeding pipe 7, one end of the feeding pipe 7 passes through the tank cover 2 and is fixedly installed with a mounting block 9, two movable grooves are arranged inside the mounting block 9, a feeding port is arranged at the bottom of the two movable grooves and at the bottom of the mounting block 9, a first electric push rod 10 is arranged inside the two movable grooves, a collecting block 11 is fixedly installed on one side of the first electric push rod 10, a mounting groove is arranged at the bottom of the tank cover 2, and two mounting plates 1 are arranged at the bottom of the mounting groove and at the bottom of the tank cover 2 2. A disinfection box 13 is provided on the top of the two mounting plates 12, and disinfectant is provided inside the disinfection box 13. One side of the disinfection box 13 is fixedly installed with the inner wall of the mounting groove. A telescopic tube 14 is fixedly installed on one side of the disinfection box 13. A nozzle is rotatably connected to one end of the telescopic tube 14. A control valve is fixedly installed on one end of the telescopic tube 14 close to the disinfection box 13. A movable structure 15 is provided on one side of the disinfection box 13 and the telescopic tube 14 and located inside the mounting groove. A groove is provided on the side of the mounting groove away from the disinfection box 13, and a connecting port is provided in the middle of the groove. The connecting port runs through one side of the feeding pipe 7. Sealing blocks 16 are slidably connected to the inner walls of the upper and lower sides of the groove. The sealing blocks 16 are arranged corresponding to the connecting port. A second electric push rod 17 is fixedly installed on one side of the sealing block 16, and one side of the second electric push rod 17 is fixedly installed on the inner wall of the groove away from the sealing block 16.

[0019] The seed liquid is added into the fermentation tank 1 in a certain proportion through the feed pipe 3, and the stirring motor 4 is started to drive the connecting rod 5, the stirring rod 6 and the stirring block to stir, and the stirring speed is controlled, and the temperature, pH value, dissolved oxygen content and other parameters are controlled so that the seed liquid is stirred evenly inside the fermentation tank 1 for fermentation. When it is necessary to increase the carbon source and nitrogen source, the first electric push rod 10 is started to push the collecting block 11 to move, so that the collecting block 11 moves to the top of the feeding port and is located at the bottom of the feeding pipe 7, and then the second electric push rod 17 is started to shrink and drive the sealing block 16 to move, so that the sealing block 16 is away from the connecting port and the connecting port is exposed, and then the telescopic tube 14 is extended through the moving structure 15, so that one end of the telescopic tube 14 passes through the connecting port and is connected to the feeding pipe 7, and the disinfectant inside the disinfection box 13 is transported from the telescopic tube 14 to the inside of the feeding pipe 7 through the control valve, and the inner wall of the feeding pipe 7 is disinfected by the rotation of the nozzle, so that the inner wall of the feeding pipe 7 is thoroughly sterilized, and the disinfection of the collecting block 11 is carried out. The venom is collected. After the disinfection is completed, the telescopic tube 14 and the nozzle are driven to move to the inside of the installation groove by the moving structure 15, and then the second electric push rod 17 is used to drive the sealing block 16 to keep the connection port sealed. The first electric push rod 10 drives the collecting block 11 to move to one side of the inside of the moving groove to expose the feeding port, and then open the sealing cover 8, and add the required feeding material to the inside of the fermentation tank 1 through the feeding port of the feeding pipe 7 to facilitate the preparation of arginine. After the feeding is added, the second electric rod pusher 17 and the sealing block 16 are moved, and the mobile structure 15 moves the telescopic tube 14 and the nozzle to the moving port, and the feeding pipe 7 is disinfected, so that no bacteria, molds and other microorganisms will remain in the feeding pipe 7, and these microorganisms are prevented from entering with the feeding, thereby facilitating the improvement of the yield and quality of arginine.

[0020] like Figure 1 to Figure 2 As shown, a support frame 18 is arranged on the outside of the connecting rod 5, the bottom of the support frame 18 is fixedly mounted on the top of the tank cover 2, the bottom of the stirring motor 4 is fixedly mounted on the top of the support frame 18, the feeding pipe 7 is located on both sides of the support frame 18, the connecting rod 5 and the stirring rod 6 extend through the tank cover 2 and the mounting block 9 to the inside of the fermenter 1, the mounting block 9 is located at the top inside the fermenter 1, and it is mounted on the inner wall of the fermenter 1.

[0021] The support frame 18 is convenient for installing the stirring motor 4, so that the stirring motor 4 drives the connecting rod 5, the stirring rod 6 and the stirring block to rotate, thereby improving the stability of the stirring motor 4, the connecting rod 5 and the stirring rotation, so as to facilitate the uniform stirring of the seed liquid inside the fermentation tank 1 and improve the fermentation effect. The installation block 9 is connected with the feeding pipe 7, and the first electric push rod 10 is convenient for pushing the collecting block 11 to move through the movable groove, so that the disinfectant after disinfection of the inner wall of the feeding pipe 7 can be collected, so that the feeding pipe 7 can be disinfected and sterilized, and the residual bacteria, molds and other microorganisms in the feeding pipe 7 are prevented, which affects the yield and quality of arginine.

[0022] like Figures 3 to 6 As shown, the feeding port is located on both sides of the stirring rod 6, a fixed plate is inserted into one side of the first electric push rod 10, the fixed plate is fixedly installed on the inner wall of the movable groove, the collecting block 11 is located on one side of the feeding port, and a movable port is opened on the side of the mounting block 9 close to the collecting block 11, and a protective door 19 is hinged at the movable port, and the fixed plate, the first electric push rod 10, the collecting block 11 and the protective door 19 are all symmetrically arranged with respect to the center of the mounting block 9.

[0023] It is convenient to add different feeds to the fermenter 1 at the same time through the two feed ports and the feed pipe 7, and it is convenient to install the first electric push rod 10 through the fixed plate. The first electric push rod 10 pushes the collecting block 11 to move, so that the collecting block 11 can collect the disinfectant after use, and can move to the side of the feed port after collection, and will not hinder the feed from entering the fermenter 1. When the mounting block 9 is taken out of the fermenter 1, the protective door 19 is rotated to separate the first electric push rod 10 from the fixed plate, and the first electric push rod 10 and the collecting block 11 are taken out of the mobile tank, the disinfectant in the collecting block 11 is treated, and then put into the mobile tank for further collection, thereby facilitating the use of the collecting block 11.

[0024] like Figures 3 to 6 As shown, a sealing plate 20 is hinged at the bottom of the mounting block 9 and located at the bottom of the feeding port, a connecting spring 21 is fixedly installed on the top of one side of the sealing plate 20, and a fixing hole is opened at the bottom of the mounting block 9 and located on one side of the feeding port, and one end of the connecting spring 21 is fixedly installed on the inner wall of the fixing hole.

[0025] When the feed material enters the mounting block 9 through the feed material pipe 7, the feed material falls onto the sealing plate 20, causing one end of the sealing plate 20 to deflect downward, and causing the connecting spring 21 to stretch, so that the feed material moves downward along the inclined sealing plate 20 to the interior of the fermenter 1. When the feed material is added, the sealing plate 20 is no longer squeezed by the weight of the feed material, and under the contraction of the connecting spring 21, the sealing plate 20 moves to the bottom of the mounting block 9, and the connecting spring 21 contracts to the inside of the fixed hole, blocking and sealing the feed material port, further improving the sealing performance of the feed material port, thereby facilitating improving the fermentation effect of the fermenter 1.

[0026] like Figures 7 to 9As shown, the moving structure 15 includes a servo motor 151, and the output end of the servo motor 151 is transmission-connected with a rotating rod 152, and a gear 153 is fixedly installed at one end of the rotating rod 152. The first rack 153 and the second rack 154 are respectively meshed on both sides of the gear 153. A connecting plate 155 is fixedly installed on one side of the first rack 153 and the second rack 154. One end of the connecting plate 155 is fixedly installed on the telescopic tube 14, and the shape of the mounting groove is U-shaped. The top of the servo motor 151 is fixedly installed on the top inner wall of the mounting groove, and the bottom of the rotating rod 152 is rotatably connected to the bottom inner wall of the mounting groove. One side of the first rack 153 and the second rack 154 are both slidably connected to the inner wall of the moving groove, and the top of the connecting plate 155 is fixedly installed with a moving rod 22, and the top of the moving rod 22 is slidably connected to the inner wall of the top of the mounting groove. The first rack 153, the second rack 154, the connecting plate 155, the moving rod 22, the disinfection box 13 and the telescopic tube 14 are symmetrically arranged with the center of the tank cover 2.

[0027] Start the servo motor 151 to drive the rotating rod 152 and the gear 153 to rotate, so that the first rack 153 and the second rack 154 move away from each other along the inner wall of the installation groove, and the first rack 153 and the second rack 154 drive the connecting plate 155 to move in the direction away from the gear 153, and the connecting plate 155 drives the moving rod 22 to move along the inner wall at the top of the installation groove, so as to improve the stability of the connecting plate 155 when connected, and the connecting plate 155 drives the telescopic tube 14 to extend, so that the nozzle moves to the inside of the feeding pipe 7 through the connecting port, and the U-shaped installation groove makes it convenient for the first rack 153 and the second rack 154 to drive the connecting plate 155 to move at the same time, and through the symmetrical arrangement of the first rack 153, the second rack 154, the connecting plate 155, the moving rod 22, the disinfection box 13 and the telescopic tube 14, the two feeding pipes 7 can be disinfected at the same time, thereby improving the feeding efficiency.

[0028] like Figure 5 As shown, a sealing ring 23 is fixedly installed on the outer side of one end of the feeding pipe 7 close to the mounting block 9, and a clamping plate 24 is arranged on the outer side of the sealing ring 23. One side of the clamping plate 24 is arc-shaped, and the arc-shaped side of the clamping plate 24 fits with the outer side of the sealing ring 23. An electric telescopic rod 25 is fixedly installed on one side of the clamping plate 24, and a fixing block 26 is fixedly installed on one side of the electric telescopic rod 25. The bottom of the fixing block 26 is fixedly installed on the top of the mounting block 9, and the fixing block 26, the electric telescopic rod 25 and the clamping plate 24 are distributed in a circular array with the sealing ring 23 as the center.

[0029] The sealing ring 23 facilitates the sealing of the connection between the feeding tube 7 and the mounting block 9, and the fixing block 26 facilitates the installation of the electric telescopic rod 25. By starting the electric telescopic rod 25, the clamping plate 24 is pushed to move, so that the clamping plate 24 fits against the outer side of the sealing ring 23, so that the sealing ring 23 is kept tightly connected between the feeding tube 7 and the mounting block 9. The fixing blocks 26, the electric telescopic rod 25 and the clamping plate 24 distributed in a circular array can improve the sealing performance of the sealing ring 23 to prevent the connection between the feeding tube 7 and the mounting block 9 from being poorly sealed, which may cause microorganisms in the outside air to enter the feeding tube 7 and then contaminate the feeding tube 7.

[0030] The invention also discloses an application of an arginine preparation process for improving skin moisturizing ability, which is applied to emulsions, water, creams, essences, facial masks, soaps, shampoos, conditioners, shower gels, cleansers, etc. in cosmetics.

[0031] Working principle: Select appropriate strains, inoculate the preserved strains into the seed culture medium containing rich nutrients, and cultivate them at suitable temperature, rotation speed and ventilation volume to activate the strains and multiply them in large quantities. After the reproduction is completed, add the cultured seed liquid into the fermentation tank 1 in a certain proportion, start the stirring motor 4 to drive the connecting rod 5, stirring rod 6 and stirring block to stir, control the stirring speed, and control the temperature and pH. The parameters such as value and dissolved oxygen content are set so that the seed liquid is stirred evenly inside the fermentation tank 1 for fermentation. When it is necessary to increase the carbon source and nitrogen source, the first electric push rod 10 is started to push the collecting block 11 to move, so that the collecting block 11 moves to the top of the feeding port and is located at the bottom of the feeding pipe 7. The second electric push rod 17 is then started to contract and drive the sealing block 16 to move, so that the sealing block 16 is away from the connecting port to expose the connecting port. The servo motor 151 is then started to drive the rotating rod 152 and the gear 153 to rotate, so that the first rack 153 and the second rack 154 move away from each other along the inner wall of the installation groove. The first rack 153 and the second rack 154 drive the connecting plate 155 to move in the direction away from the gear 153. The connecting plate 155 drives the moving rod 22 to move along the inner wall of the top of the installation groove. The connecting plate 155 drives the telescopic tube 14 to extend, so that the nozzle moves to the inside of the feeding pipe 7 through the connecting port. The disinfectant in the disinfection box 13 is discharged from the telescopic tube 14 through the control valve. The liquid is transported to the inside of the feeding pipe 7, and the inner wall of the feeding pipe 7 is disinfected by the rotation of the nozzle, so that the inner wall of the feeding pipe 7 is thoroughly sterilized, and the liquid is collected by the disinfectant of the collecting block 11. After the disinfection is completed, the servo motor 151 is rotated, and the telescopic tube 14 and the nozzle are moved to the inside of the installation groove under the action of the first rack 153 and the second rack 154, and the second electric push rod 17 is used to drive the sealing block 16 to keep the connection port sealed, and the collecting block 11 is driven by the first electric push rod 10 to move to one side of the inside of the moving groove to expose the feeding port, and then the sealing cover 8 is opened, and the required feeding material is added to the inside of the fermentation tank 1 through the feeding port of the feeding pipe 7, so as to facilitate the preparation of arginine. After the feeding is added, the second electric rod push 17 and the sealing block 16 are moved, and the mobile structure 15 moves the telescopic tube 14 and the nozzle to the moving port, and the feeding pipe 7 is disinfected, so that no bacteria, molds and other microorganisms will remain in the feeding pipe 7.

[0032] When the feed enters the mounting block 9 through the feed pipe 7, the feed falls onto the sealing plate 20, causing one end of the sealing plate 20 to deflect downward, and causing the connecting spring 21 to stretch, so that the feed moves downward along the inclined sealing plate 20 to the interior of the fermenter 1. When the feed is added, the sealing plate 20 is no longer squeezed by the weight of the feed, and under the contraction of the connecting spring 21, the sealing plate 20 moves to the bottom of the mounting block 9, and the connecting spring 21 contracts to the inside of the fixed hole, blocking and sealing the feed port, further improving the sealing of the feed port, and facilitating the sealing of the feed pipe 7 and the mounting block 9 through the sealing ring 23. By starting the electric telescopic rod 25, the tightening plate 24 is pushed to move, so that the tightening plate 24 fits against the outer side of the sealing ring 23, so that the sealing ring 23 is tightly connected between the feed pipe 7 and the mounting block 9, to prevent the feed pipe 7 from being poorly sealed at the connection between the mounting block 9, which may cause microorganisms in the outside air to enter the feed pipe 7 and then contaminate the feed pipe 7.

[0033] After the feeding is completed, fermentation is carried out. At the same time, the temperature, pH value, dissolved oxygen and other conditions must be strictly controlled during the fermentation process. The dissolved oxygen is controlled by adjusting the ventilation volume and stirring speed. As the fermentation proceeds, the microorganisms use glucose, ammonium salts and other substances in the culture medium to synthesize arginine. After the fermentation is completed, the bacteria are first removed by centrifugation or filtration to obtain a fermentation liquid containing arginine. Then, the ion exchange resin method is used to use the charged properties of arginine at different pH values ​​to adsorb it on the ion exchange resin, and then it is eluted with a suitable eluent. Arginine is further purified through operations such as concentration and crystallization.

[0034] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them.

Claims

1. A process for preparing arginine for improving skin moisturizing ability, characterized in that: The steps include: Step 1: Add commonly used glucose, sucrose and other sugar substances to the culture medium. The content of glucose in the culture medium is 5% to 15% (mass fraction). The amount of ammonia water added can be added in appropriate amounts according to the pH value control requirements during the fermentation process. The content of ammonium sulfate can be around 1% to 3%. Add inorganic salts: including potassium dihydrogen phosphate (0.1% to 0.3%), magnesium sulfate (0.05% to 0.1%), etc., and add appropriate amounts of biotin (generally at a concentration of 5-20μg / L) and other growth factors; Step 2: First, inoculate the strain into a seed shake bottle or seed tank filled with seed culture medium, and culture it at a suitable temperature and speed for 12-24 hours to obtain a seed solution with vigorous growth and good vitality; Step 3: Add the seed liquid to the fermentation tank (1) in a certain proportion (usually 5%-15% by volume), maintain the pH value between 6.5 and 7.5 by automatically adding ammonia water, and maintain the dissolved oxygen content at 20%-30% saturation. The fermentation time lasts for about 48-72 hours, and the temperature, pH value, dissolved oxygen content and other parameters are well controlled; Step 4: After the fermentation is completed, remove impurities such as bacteria by filtration or centrifugation to obtain a fermentation broth containing L-arginine. Then, use a strong acid cation exchange resin to first adsorb L-arginine on the fermentation broth, then elute it with a suitable eluent, and then concentrate and crystallize it to obtain a relatively pure L-arginine.

2. A process for preparing arginine for improving skin moisturizing ability according to claim 1, characterized in that: In step 3, the fermentation tank (1) is provided with a tank cover (2) on the top, a feeding pipe (3) is provided on one side of the top of the tank cover (2), a stirring motor (4) is provided in the middle of the top of the tank cover (2), the output end of the stirring motor (4) is connected to a connecting rod (5) in a transmission manner, a stirring rod (6) is fixedly installed on one end of the connecting rod (5), a stirring block is fixedly installed on the outside of the stirring rod (6), a feeding pipe (7) is provided on both sides of the left and right sides of the connecting rod (5), a sealing cover (8) is provided on the top of the feeding pipe (7), one end of the feeding pipe (7) passes through the tank cover (2) and is fixedly installed with a mounting block (9), two movable grooves are provided inside the mounting block (9), a feeding port is provided at the bottom of the two movable grooves and located at the bottom of the mounting block (9), a first electric push rod (10) is provided inside the two movable grooves, a collecting block (11) is fixedly installed on one side of the first electric push rod (10), a mounting groove is provided at the bottom of the tank cover (2), and two mounting The mounting plate (12) comprises a disinfection box (13) on the top of each of the two mounting plates (12), a disinfectant being arranged inside the disinfection box (13), one side of the disinfection box (13) being fixedly mounted to the inner wall of the mounting groove, a telescopic tube (14) being fixedly mounted on one side of the disinfection box (13), one end of the telescopic tube (14) being rotatably connected to a spray head, a control valve being fixedly mounted on one end of the telescopic tube (14) close to the disinfection box (13), and the disinfection box (13) and one side of the telescopic tube (14) being located inside the mounting groove. A movable structure (15) is provided, the installation slot is provided with a groove on the side away from the disinfection box (13), a connection port is provided in the middle of the groove, the connection port penetrates one side of the feeding pipe (7), the inner walls on both upper and lower sides of the groove are slidably connected with sealing blocks (16), the sealing blocks (16) are arranged corresponding to the connection port, a second electric push rod (17) is fixedly installed on one side of the sealing block (16), and one side of the second electric push rod (17) is fixedly installed on the inner wall of the groove on the side away from the sealing block (16).

3. A process for preparing arginine for improving skin moisturizing ability according to claim 2, characterized in that: A support frame (18) is arranged outside the connecting rod (5); the bottom of the support frame (18) is fixedly mounted on the top of the tank cover (2); the bottom of the stirring motor (4) is fixedly mounted on the top of the support frame (18); the feeding pipe (7) is located on both sides of the support frame (18); the connecting rod (5) and the stirring rod (6) pass through the tank cover (2) and the mounting block (9) to extend into the fermentation tank (1); the mounting block (9) is located at the top of the fermentation tank (1) and is mounted on the inner wall of the fermentation tank (1).

4. A process for preparing arginine for improving skin moisturizing ability according to claim 2, characterized in that: The feeding port is located on both sides of the stirring rod (6); a fixed plate is inserted into one side of the first electric push rod (10); the fixed plate is fixedly mounted on the inner wall of the movable groove; the collecting block (11) is located on one side of the feeding port; a movable port is opened on the side of the mounting block (9) close to the collecting block (11); a protective door (19) is hinged at the movable port; the fixed plate, the first electric push rod (10), the collecting block (11) and the protective door (19) are all symmetrically arranged around the center of the mounting block (9).

5. The process for preparing arginine for improving skin moisturizing ability according to claim 2, characterized in that: A sealing plate (20) is hingedly connected to the bottom of the mounting block (9) and located at the bottom of the feeding port, a connecting spring (21) is fixedly mounted on the top of one side of the sealing plate (20), a fixing hole is opened at the bottom of the mounting block (9) and located on one side of the feeding port, and one end of the connecting spring (21) is fixedly mounted to the inner wall of the fixing hole.

6. A process for preparing arginine for improving skin moisturizing ability according to claim 2, characterized in that: The moving structure (15) comprises a servo motor (151), the output end of the servo motor (151) is drivingly connected to a rotating rod (152), one end of the rotating rod (152) is fixedly mounted with a gear (153), both sides of the gear (153) are respectively meshed with a first rack (153) and a second rack (154), one side of the first rack (153) and the second rack (154) are both fixedly mounted with a connecting plate (155), and one end of the connecting plate (155) is fixedly mounted to the telescopic tube (14).

7. A process for preparing arginine for improving skin moisturizing ability according to claim 6, characterized in that: The shape of the mounting groove is U-shaped, the top of the servo motor (151) is fixedly mounted on the inner wall of the top of the mounting groove, the bottom of the rotating rod (152) is rotatably connected to the inner wall of the bottom of the mounting groove, one side of the first rack (153) and the second rack (154) are both slidably connected to the inner wall of the movable groove, the top of the connecting plate (155) is fixedly mounted with a movable rod (22), the top of the movable rod (22) is slidably connected to the inner wall of the top of the mounting groove, and the first rack (153), the second rack (154), the connecting plate (155), the movable rod (22), the disinfection box (13) and the telescopic tube (14) are symmetrically arranged around the center of the tank cover (2).

8. The process for preparing arginine for improving skin moisturizing ability according to claim 2, characterized in that: A sealing ring (23) is fixedly mounted on the outer side of one end of the feeding pipe (7) close to the mounting block (9); a clamping plate (24) is arranged on the outer side of the sealing ring (23); one side of the clamping plate (24) is arc-shaped; the arc-shaped side of the clamping plate (24) is in contact with the outer side of the sealing ring (23); an electric telescopic rod (25) is fixedly mounted on one side of the clamping plate (24); a fixing block (26) is fixedly mounted on one side of the electric telescopic rod (25); the bottom of the fixing block (26) is fixedly mounted on the top of the mounting block (9); and the fixing block (26), the electric telescopic rod (25) and the clamping plate (24) are distributed in a circular array with the sealing ring (23) at the center.

9. An application of the arginine preparation process for improving skin moisturizing ability according to any one of claims 1 to 8, characterized in that: Used in lotions, water, creams, essences, facial masks, soaps, shampoos, conditioners, shower gels and cleansers in cosmetics.

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