Pesticide composition as well as preparation method and application thereof

Abstract

The invention provides a pesticide composition as well as a preparation method and application thereof, and belongs to the technical field of pesticides, the active ingredients of the pesticide composition comprise acetamido abamectin B2a and fluopyram, and the weight ratio of the acetamido abamectin B2a to the fluopyram is (30: 1)-(1: 30); the preparation method comprises the following steps: mixing the acetamido abamectin B2a, the fluopyram, the wetting dispersant, the thickening agent, the anti-freezing agent, the defoaming agent and the water according to a preset proportion, carrying out wet sanding, and grinding until the particle size D90 is less than or equal to 5 microns, so as to obtain the water suspending agent. The composition is used for preventing and treating root nematodes of vegetables, cereals and flowers, and the application mode is hole application, furrow application or root irrigation. The pesticide composition disclosed by the invention is efficient, low in toxicity and environment-friendly, the combination of the two medicaments has a remarkable synergistic effect, the nematicidal activity is improved, the use amount of the pesticide can be effectively reduced, and the pesticide composition has a very high social value.

Description

Technical Field

[0001] The invention relates to a pesticide composition, in particular to a nematicide pesticide composition containing avermectin B2a and application thereof, belonging to the technical field of pesticides. Background Art

[0002] Avermectin is produced by Sterptomyces avermitills, which is a type of streptomycin and belongs to gray streptomycin. Avermectin is a mixture of fermented components, with a total of 8 components: A1a, A1b, A2a, A2b, B1a, B1b, B2a, B2b. At present, avermectin is mainly used as a pesticide insecticide, and can also be used as a veterinary anthelmintic. Its mode of action is mainly through acting on the GABA A receptors of insect neuron synapses or neuromuscular synapses, interfering with the information transmission of nerve endings in the insect body, that is, stimulating the nerve endings to release the neurotransmission inhibitor γ-aminobutyric acid (GABA), prompting the GABA-gated chloride ion channel to extend the opening, and has an activating effect on the chloride ion channel. A large amount of chloride ions influx causes the nerve membrane potential to be supercharged, causing the nerve membrane to be in an inhibited state, thereby blocking the connection between the nerve endings and the muscles, causing the insect to be paralyzed, refuse to eat, and die. Emamectin B2a is a derivative of avermectin. The latest research shows that emamectin B2a has a certain preventive effect on nematodes in plant roots. Whether it has a synergistic effect in combination with other nematicides still needs further study.

[0003] Fluopyram is a new type of pesticide discovered by Bayer in 2002 and launched in 2012. The product belongs to the category of succinate dehydrogenase inhibitor fungicides and nematicides. It is also used as seed treatment agent and agricultural product storage preservative. The mechanism of action of fluopyram is to inhibit the activity of succinate dehydrogenase in the electron transport chain of pathogenic bacteria mitochondrial respiration and tricarboxylic acid cycle, interfere with respiration, hinder energy production, and thus kill pathogenic bacteria. Fluopyram is not only a broad-spectrum, systemic fungicide, but also the first nematicide to inhibit complex II. It represents a new type of nematicide with a new mechanism of action and has a good market prospect. Fluopyram can effectively prevent the invasion of nematodes. It is the first SDHI nematicide and the first nematicide with low toxicity to users and the environment. Although fluopyram products have excellent properties and outstanding characteristics, the inherent resistance risk of SDHI fungicides is classified as medium to high by FRAC. Summary of the invention

[0004] In view of the above problems, the present invention provides a pesticide composition, a preparation method and application thereof to improve nematicidal activity.

[0005] To achieve the above object, the technical solution of the present invention is: a pesticide composition, the active ingredients of the pesticide composition are composed of avermectin B2a and fluopyram, and the weight ratio of avermectin B2a to fluopyram is 30:1-1:30.

[0006] Furthermore, the weight ratio of avermectin B2a to fluopyram is 1:1.

[0007] Furthermore, the pesticide composition is an aqueous suspension concentrate, which includes avermectin B2a, fluopyram, a wetting and dispersing agent, a thickener, an antifreeze agent and a defoaming agent.

[0008] Furthermore, the aqueous suspension comprises, by weight percentage, 1-30% of avermectin B2a, 1-30% of fluopyram, 1-10% of a wetting and dispersing agent, 0.5-3% of a thickener, 2-6% of an antifreeze agent, 0.1-1% of a defoaming agent, and the water content is 100%.

[0009] Furthermore, the wetting and dispersing agent is 1-3 of tristyryl polyoxyethylene ether phosphate, polycarboxylate, fatty alcohol polyoxyethylene ether, EO / PO block polyether, lignin sulfonate, naphthalene sulfonate, and dioctyl sodium sulfosuccinate.

[0010] Furthermore, the thickener is 1-3 of magnesium aluminum silicate, white carbon black, xanthan gum, sodium carboxymethyl cellulose, gum arabic and polyethylene glycol.

[0011] Furthermore, the antifreeze agent is 1-2 of ethylene glycol, propylene glycol, glycerin and urea.

[0012] Furthermore, the defoaming agent is a silicone defoaming agent.

[0013] The preparation method of the above-mentioned pesticide composition comprises mixing avermectin B2a, fluopyram, a wetting and dispersing agent, a thickener, an antifreeze agent, a defoaming agent and water in a predetermined proportion, and grinding the mixture by wet sand milling to obtain an aqueous suspension with a particle size D90≤5 μm.

[0014] The above pesticide composition is used for preventing and controlling root nematodes of vegetables, cereals and flowers, and the application method is hole application, furrow application or root irrigation.

[0015] The beneficial effects of a pesticide composition, a preparation method and application thereof of the present invention are as follows:

[0016] The pesticide composition of the present invention is highly effective, low in toxicity, and environmentally friendly. The combination of the two agents has a significant synergistic effect, improves the nematicidal activity, and can also effectively reduce the amount of pesticide used, which complies with the national double reduction measures and has high social value.

[0017] The present invention combines acetaminophen B2a and fluopyram, which has good synergistic effect, and the effect is obviously better than that of a single agent. At the same time, the effective ingredients of the nematicidal composition have different action mechanisms, which reduces the resistance produced under a single action and delays the production of nematode resistance to a single agent. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0019] Figure 1 This is a result diagram of the root-knot nematode damage on the cucumber roots after being treated with different agents of the present invention. DETAILED DESCRIPTION

[0020] The technical scheme in the embodiment of the present invention is described clearly and completely below in conjunction with the accompanying drawings in the embodiment of the present invention. In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention can also be implemented in other ways different from those described herein, and those skilled in the art can make similar generalizations without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0021] A pesticide composition, wherein the active ingredients of the pesticide composition are composed of avermectin B2a and fluopyram, wherein the weight ratio of avermectin B2a to fluopyram is 30:1-1:30. Preferably, the weight ratio of avermectin B2a to fluopyram is 1:1.

[0022] Preferably, the pesticide composition is an aqueous suspension, and the aqueous suspension comprises avermectin B2a, fluopyram, a wetting and dispersing agent, a thickener, an antifreeze agent and a defoamer. Further preferably, the aqueous suspension comprises avermectin B2a 1-30%, fluopyram 1-30%, a wetting and dispersing agent 1-10%, a thickener 0.5-3%, an antifreeze agent 2-6%, a defoamer 0.1-1%, and water makes up 100%.

[0023] Preferably, the wetting and dispersing agent is 1-3 of tristyryl polyoxyethylene ether phosphate, polycarboxylate, fatty alcohol polyoxyethylene ether, EO / PO block polyether, lignin sulfonate, naphthalene sulfonate, and sodium dioctyl sulfosuccinate; the thickener is 1-3 of magnesium aluminum silicate, white carbon black, xanthan gum, sodium carboxymethyl cellulose, gum arabic and polyethylene glycol; the antifreeze agent is 1-2 of ethylene glycol, propylene glycol, glycerin, and urea; and the defoamer is a silicone defoamer.

[0024] The preparation method of the above pesticide composition is to mix avermectin B2a, fluopyram, a wetting and dispersing agent, a thickener, an antifreeze agent, a defoaming agent and water in a predetermined proportion, grind them by wet sand milling to obtain an aqueous suspension with a particle size D90≤5μm.

[0025] The above pesticide composition is used for preventing and controlling root nematodes of vegetables, cereals and flowers, and the application method is hole application, furrow application or root irrigation.

[0026] Example 1: 5% avermectin + 10% fluopyram aqueous suspension

[0027] 5% avermectin B2a, 10% fluopyram, 3% tristyryl polyoxyethylene ether phosphate, 1% naphthalene sulfonate, 0.5% EO / PO block polyether, 0.25% xanthan gum, 0.4% silicone defoamer, 5% propylene glycol, water is supplemented to 100%, and the particle size D90≤5um is ground into a water suspension concentrate by wet sand milling.

[0028] Example 2: 5% avermectin B2a + 5% fluopyram aqueous suspension

[0029] 5% acetamidoavermectin B2a, 5% fluopyram, 3% tristyryl polyoxyethylene ether phosphate, 1% polycarboxylate, 1% fatty alcohol polyoxyethylene ether, 1.5% magnesium aluminum silicate, 0.25% xanthan gum, 0.4% organosilicon defoamer, 5% propylene glycol, water is supplemented to 100%, and the particle size D90≤5um is ground into a water suspension by wet sand milling.

[0030] Example 3: 10% avermectin B2a + 5% fluopyram aqueous suspension

[0031] 10% avermectin B2a, 5% fluopyram, 3% tristyryl polyoxyethylene ether phosphate, 0.5% lignin sulfonate, 1% fatty alcohol polyoxyethylene ether, 1% white carbon black, 0.22% xanthan gum, 0.4% organosilicon defoamer, 5% ethylene glycol, water is added to 100%, and the mixture is ground into a water suspension with a particle size of D90≤5um by wet sand milling.

[0032] Example 4: 15% avermectin B2a + 5% fluopyram aqueous suspension

[0033] 15% acetamidoavermectin B2a, 5% fluopyram, 3% tristyryl polyoxyethylene ether phosphate, 1% lignin sulfonate, 1% sodium dioctyl sulfosuccinate, 1.5% sodium carboxymethyl cellulose, 0.2% xanthan gum, 0.4% organosilicon defoamer, 2% urea, 3% glycerol, water is added to 100%, and the mixture is ground into a water suspension with a particle size of D90≤5um by wet sand milling.

[0034] Example 5: 5% avermectin B2a + 15% fluopyram aqueous suspension

[0035] 5% acetylaminoavermectin B2a, 15% fluopyram, 3% tristyryl polyoxyethylene ether phosphate, 0.5% lignin sulfonate, 1% polycarboxylate, 1.5% sodium carboxymethyl cellulose, 0.15% xanthan gum, 0.4% organosilicon defoamer, 2% ethylene glycol, 3% urea, water is added to 100%, and the mixture is ground into a water suspension with a particle size of D90≤5um by wet sand milling.

[0036] Example 6: 1% avermectin B2a + 30% fluopyram aqueous suspension

[0037] 1% acetamidoavermectin B2a, 30% fluopyram, 3% tristyryl polyoxyethylene ether phosphate, 1% lignin sulfonate, 1.5% polycarboxylate, 1% sodium carboxymethyl cellulose, 0.1% xanthan gum, 0.4% organosilicon defoamer, 2% propylene glycol, 1% urea, water is added to 100%, and the mixture is ground into a water suspension with a particle size of D90≤5um by wet sand milling.

[0038] Example 7: 30% avermectin B2a + 1% fluopyram aqueous suspension

[0039] 30% avermectin B2a, 1% fluopyram, 3% EO / PO block polyether, 1.5% lignin sulfonate, 2% polycarboxylate, 1.5% sodium carboxymethyl cellulose, 0.1% xanthan gum, 0.8% organosilicon defoamer, 2% propylene glycol, 2% urea, water is supplemented to 100%, and the mixture is ground to a water suspension with a particle size of D90≤5um by wet sand milling.

[0040] Application test examples

[0041] The pesticide composition prepared in Examples 1 and 2 was applied to the prevention and treatment of cucumber root-knot nematode disease, specifically as follows:

[0042] One week after the cucumber seedlings were transplanted, the roots were irrigated with each treatment (as shown in Table 1 below). After the cucumber harvest period, a five-point sampling method was used in each treatment area, and 4 plants were selected at each point. The number of diseased plants at each level in each treatment was counted, and then the disease index and control effect of different treatments were calculated. The results are shown in Tables 1 and Figure 1 shown.

[0043]

[0044] Diseased plant classification standards:

[0045] Level 0: No root knots found at the root

[0046] Level 1: Few fibrous roots, no taproot

[0047] The second-level fibrous roots have larger root knots, and the taproot has no

[0048] Level 3: 50% of the roots have knots, and some taproots have knots

[0049] Level 4 taproot has more knots

[0050] Level 5: All taproots have root knots

[0051] CK disease index: disease index of the clean water control group

[0052] Table 1: Effect of nematicidal composition water suspension concentrate on cucumber root-knot nematodes

[0053]

[0054] Synergistic effect verification test example

[0055] Toxicity determination of avermectin B2a and fluopyram against southern root-knot nematode:

[0056] The test was conducted in accordance with NY / T 1833.1-2009 "Guidelines for Indoor Bioassay Tests of Pesticides - Nematicides" Part 1: Insect immersion method for inhibition of plant pathogenic nematodes.

[0057] Fresh plant roots infected with root-knot nematodes were cleaned, cut into 1 cm long segments, put into a tissue crusher with 3 times the amount of water and crushed for 30 seconds, transferred to a combination of 200 mesh (pore size 75 μm) and 500 mesh (pore size 26 μm) standard sieves, and the plant tissues in the sieves were rinsed with tap water. The nematode eggs in the 500-mesh standard sieve were collected and surface disinfected with a 0.5% NaClO solution for 3 minutes. After rinsing with sterile water for 5 times, they were placed in a homemade Behman funnel and cultured in a dark incubator at 25°C. The southern root-knot nematodes were collected every 24 hours for later use.

[0058] According to the experimental design, the test agent was gradiently diluted with a 0.1% Triton aqueous solution, and a 0.1% Triton aqueous solution was used as a control. The collected root-knot nematodes hatched within 24 hours were made into a suspension (about 200 / mL) with clean water. 0.5mL of the drug solution and 0.5mL of the nematode suspension were transferred with a pipette and placed in a 24-well plate. The plate was repeated 3 times. After mixing with a thin glass rod, the plate was placed in a 25°C constant temperature incubator for incubation in the dark. After 72 hours, the total number of nematodes and the number of dead nematodes were checked, and the mortality rate and corrected mortality rate were calculated.

[0059] The nematode death was determined by needle pricking under a stereo microscope, the number of dead nematodes was counted, and the nematode mortality rate and corrected mortality rate were calculated using the following formula:

[0060] Mortality (%) = number of dead nematodes / number of treated nematodes × 100%

[0061] Corrected mortality rate (%) = (treatment mortality rate - control mortality rate) / (100 - control mortality rate) × 100%

[0062] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 15%, the corrected mortality rate should be calculated according to the formula; if the control mortality rate is greater than 15%, the test needs to be repeated.

[0063] The concentration used in the test is the x-axis, and the mortality rate corresponding to the survival probability value (obtained from the biostatistical probability value conversion table) is the y-axis. A linear regression equation is established to obtain the toxicity regression equation. Substitute the mortality rate of 50% (i.e., the survival probability value is 5) into the toxicity regression equation to obtain the LC value of the drug. 50 value.

[0064] Evaluation of the combined effect of avermectin B2a and fluopyram on nematodes by the co-toxicity coefficient method (fluopyram as the standard agent)

[0065] Measured toxicity index (ATI) = (standard agent LC 50 / Test reagent LC 50 )×100

[0066] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage of A in the mixture + toxicity index of agent B × percentage of B in the mixture

[0067] Co-toxicity coefficient (CTC) = (ATI / TTI) × 100

[0068] A co-toxicity coefficient CTC>120 indicates a synergistic effect, a co-toxicity coefficient between 80≤CTC≤120 indicates an additive effect, and a co-toxicity coefficient <80 indicates an antagonistic effect.

[0069] When the observed control effect exceeds the expected control effect, it indicates a synergistic effect.

[0070] The results of the toxicity test are shown in the following table:

[0071] Table 2: Results of indoor toxicity test of single active ingredients against southern root-knot nematode

[0072]

[0073] Table 3: Toxicity test results of avermectin B2a and fluopyram

[0074]

[0075]

[0076] The test results show that the combination of avermectin B2a and fluopyram has a synergistic effect in controlling southern root-knot nematodes when the ratio is between 1:30 and 30:1.

[0077] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

Claims

1. A pesticide composition, characterized in that: The active ingredients of the pesticide composition consist of avermectin B2a and fluopyram, and the weight ratio of avermectin B2a to fluopyram is 30:1-1:

30.

2. A pesticide composition according to claim 1, characterized in that: The weight ratio of avermectin B2a to fluopyram is 1:

1.

3. A pesticide composition according to claim 1 or 2, characterized in that: The pesticide composition is an aqueous suspension concentrate, which comprises avermectin B2a, fluopyram, a wetting and dispersing agent, a thickener, an antifreeze agent and a defoaming agent.

4. A pesticide composition according to claim 3, characterized in that: The aqueous suspension comprises, by weight percentage, 1-30% of avermectin B2a, 1-30% of fluopyram, 1-10% of a wetting and dispersing agent, 0.5-3% of a thickener, 2-6% of an antifreeze agent, 0.1-1% of a defoamer, and the water content is 100%.

5. A pesticide composition according to claim 3, characterized in that: The wetting and dispersing agent is one to three of tristyryl polyoxyethylene ether phosphate, polycarboxylate, fatty alcohol polyoxyethylene ether, EO / PO block polyether, lignin sulfonate, naphthalene sulfonate, and dioctyl sodium sulfosuccinate.

6. A pesticide composition according to claim 3, characterized in that: The thickener is 1 to 3 of magnesium aluminum silicate, white carbon black, xanthan gum, sodium carboxymethyl cellulose, gum arabic and polyethylene glycol.

7. A pesticide composition according to claim 3, characterized in that: The antifreeze agent is one or two of ethylene glycol, propylene glycol, glycerin and urea.

8. A pesticide composition according to claim 3, characterized in that: The defoamer is an organosilicon defoamer.

9. The method for preparing the pesticide composition according to claims 1 to 8, characterized in that: The aqueous suspension concentrate is obtained by mixing avermectin B2a, fluopyram, a wetting and dispersing agent, a thickener, an antifreeze agent, a defoaming agent and water in a predetermined ratio, and grinding the mixture by wet sand milling to a particle size of D90≤5 μm.

10. Use of the pesticide composition according to claims 1 to 8 or the pesticide composition prepared according to claim 9, characterized in that: Used to control root nematodes of vegetables, cereals and flowers, the application method is hole application, furrow application or root irrigation.

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