Method for controlling outbreak of cyclops

Abstract

The invention discloses a method for controlling outbreak of cyclops, which comprises the following steps: planting caulerpa lentillifera and polyculture hippocampus, and adding a composite pharmaceutical composition into a culture water body; the composite pharmaceutical composition is prepared from the following components in parts by weight: 5 to 10 parts of seabuckthorn flavone, 5 to 10 parts of berberine and 6 to 12 parts of salicylic acid. By means of the method, the number of the cyclops in the babylonia breeding process is greatly reduced, and outbreak of the cyclops is restrained.

Description

Technical Field

[0001] The invention belongs to the technical field of aquatic animal breeding, and more specifically, relates to a method for controlling sword flea outbreaks. Background Art

[0002] Dongfeng snail, also known as sea pig snail, sweet snail, and southern wind snail, belongs to the phylum Mollusca, class Gastropoda, subclass Prosobranchia, order Neogastropoda, family Buccinidae, and genus Babylonia. It is the main aquaculture shellfish species in my country. Dongfeng snail is a dioecious mollusk with an elongated oval shell, a hard shell, a smooth surface, fine growth lines, and a light brown or yellowish brown shell. Dongfeng snail is widely distributed, mainly living in the sandy and muddy seabed at a depth of several meters to tens of meters in Guangdong, Guangxi, Fujian, Hainan, and Taiwan, China.

[0003] In recent years, due to the continuous expansion of aquaculture areas, the tail water discharged from aquaculture has caused the eutrophication of seawater bodies to become increasingly serious, thus triggering the massive reproduction of zooplankton, resulting in a significant increase in the number of cyclops zooplankton in the upper layer of the seawater. At the same time, the aquaculture area of ​​the baby snails has gradually expanded, and the area on the coast where wells can be drilled to extract bottom water has continued to shrink. The water situation has become tense, and it is inevitable to start using surface water (surface water). However, even if the surface water is filtered, it will still carry a large number of zooplankton, especially cyclops, which is extremely harmful to the baby snails.

[0004] Cyclops belong to the class Crustacea of ​​the phylum Arthropoda. They gather in snail ponds and use their copepods to scratch the snails or even eat them. The wounds increase the risk of snails being infected with pathogens, greatly affecting the growth, survival rate and other farming characteristics of the snails. In addition, the cyclops-like zooplankton carry a large number of microorganisms in and on their bodies, including pathogenic bacteria, which also greatly affect the survival rate, size and farming success rate of the snails. Therefore, cyclops disease has become one of the important diseases in the snail farming industry.

[0005] The drug commonly used to kill sword fleas at present is trichlorfon, but it poses potential safety risks to both the snails and humans. Any slight deviation in dosage will cause the death of farmed snails. Therefore, there is a certain risk in using trichlorfon to kill sword fleas.

[0006] The patent publication number CN105660721A discloses a Chinese medicine composition for preventing and treating sword water fleas and its application method, including Chinese medicinal materials, which are measured by weight, including 10-15 parts of small arrows, 5-8 parts of sticky grass, 5-8 parts of oil orange leaves, 10-15 parts of single-legged Chinese tallow tree, and 20-30 parts of swallow tail. The Chinese medicinal materials are powdered and then mixed evenly into Chinese medicinal powder, and the Chinese medicinal powder is loaded into a plurality of medicine bags made of three-layer stockings, the medicine bags are tied tightly, and the medicine bags are placed in the fish fry tank for continuous soaking for 2-3 days. The application of this method can kill sword water fleas in the water body of sea bass fry and improve the survival rate of sea bass fry. However, this Chinese medicine composition is suitable for fish rather than Dongfeng snails, and needs to be soaked continuously for many days when used, while Dongfeng snails need continuous water breeding, and there are differences in ecological habits.

[0007] Therefore, there is an urgent need to explore a method that can improve the survival rate, size and breeding success rate of the Babylonia snail, and further, explore a method that can safely and effectively control the sword water flea. Summary of the invention

[0008] In view of the above-mentioned existing technical problems, the primary purpose of the present invention is to provide a compound pharmaceutical composition, which can significantly improve the survival rate and specifications of Babylonia snails.

[0009] The second object of the present invention is to provide a method for preparing the composite pharmaceutical composition.

[0010] The third object of the present invention is to provide a composite pharmaceutical composition for use in the preparation of a method for improving the survival rate of Babylonia snails and / or improving the specifications of Babylonia snails.

[0011] The fourth object of the present invention is to provide a use of a composite pharmaceutical composition in reducing the number of sword water fleas.

[0012] A fifth object of the present invention is to provide a method for controlling an outbreak of sword fleas in a breeding pond.

[0013] A sixth object of the present invention is to provide a method for controlling a sword water flea outbreak in a breeding pond for use in improving the survival rate of Babylonia snails and / or improving the size of Babylonia snails.

[0014] In order to achieve the above object, the present invention is implemented by the following technical solutions:

[0015] A compound medicine composition comprises, by weight, 5 to 10 parts of seabuckthorn flavonoids, 5 to 10 parts of berberine and 6 to 12 parts of salicylic acid.

[0016] In the present invention, seabuckthorn flavonoids are rich in active ingredients such as quercetin, isorhamnetin and kaempferol, which not only have the functions of enhancing the immunity of the body of the snail, improving the disease resistance of the snail, promoting the growth of the snail and improving the antioxidant capacity, but also have the remarkable characteristics of no drug resistance and no drug residue. Furthermore, berberine has multiple effects on the snail. In addition to promoting the repair of intestinal structural damage, enhancing the integrity of the intestinal mucosal barrier, and regulating the structure of intestinal flora, it also has biological functions such as enhancing the body's immunity and antioxidant capacity. Furthermore, salicylic acid has the effects of analgesia, anti-inflammatory, antibacterial and antibacterial, and can prevent and reduce the occurrence of diseases in aquaculture water or snail aquaculture.

[0017] The inventors found through research that the combined use of seabuckthorn flavonoids, berberine and salicylic acid has the effects of synergistically promoting the growth and development of baby snails, reducing feed coefficients, improving the utilization rate of nutrients, improving the composition of intestinal flora, and enhancing the body's immunity and disease resistance. It can be used for a long time throughout the entire baby snail breeding cycle, can prevent and reduce the occurrence of diseases, and avoid the occurrence of large-scale outbreaks of diseases. It can effectively improve the survival rate and specifications of baby snails.

[0018] Specifically, in the present invention, the chemical name of berberine is 5,6-dihydro-9,10-dimethoxybenzo[g]-1,3-benzodioxolane[5,6-α]quinolizine hydrochloride dihydrate. Substances with the above chemical name should be within the protection scope of the present invention, and those skilled in the art can reasonably select them.

[0019] Preferably, the compound pharmaceutical composition comprises the following components in parts by weight: 8 to 10 parts of seabuckthorn flavonoids, 7 to 10 parts of berberine, 8 to 10 parts of salicylic acid, 1 to 2 parts of calcium hydrogen phosphate and 1 to 2 parts of potassium bicarbonate.

[0020] Preferably, the compound pharmaceutical composition further comprises: 1 to 2 parts of calcium additives and / or 1 to 2 parts of potassium additives.

[0021] Preferably, the calcium additive is selected from one or more of calcium carbonate, calcium phosphate, calcium gluconate and calcium chloride.

[0022] Preferably, the potassium additive is selected from one or more of potassium chloride, potassium carbonate, potassium dihydrogen phosphate, and dipotassium hydrogen phosphate.

[0023] Furthermore, the present invention claims protection for a method for preparing a composite pharmaceutical composition, wherein seabuckthorn flavonoids, berberine and salicylic acid are uniformly mixed to obtain the composite pharmaceutical composition.

[0024] Furthermore, the present invention seeks to protect the use of the above-mentioned composite pharmaceutical composition in the preparation of a method for improving the survival rate of Babylonia snails and / or improving the specifications of Babylonia snails.

[0025] Furthermore, the present invention seeks to protect the use of the above-mentioned compound pharmaceutical composition in reducing the number of sword water fleas.

[0026] Furthermore, the present invention claims a method for controlling a sword-flea outbreak in a culture pond, wherein long-stemmed grape fern algae and seahorses are grown together, and the composite pharmaceutical composition is added to the culture water.

[0027] Preferably, the planting density of long-stem grape fern is 0.1-0.3 kg / m 2 .

[0028] Preferably, the breeding density of seahorses is 5 to 10 / m 2 .

[0029] Preferably, Babylonia snails are also cultured in the breeding pond.

[0030] Preferably, long-stem grape fern algae are planted in the aquaculture sand body, and seahorses are mixed in the aquaculture water body.

[0031] The present invention, by planting long-stem grape fern algae and co-culturing seahorses during the culture of baby snails, and using the above-mentioned composite pharmaceutical composition, greatly reduces the number of sword water fleas during the culture of baby snails, inhibits the outbreak of sword water fleas, and further improves the survival rate and specifications of baby snails based on this. Specifically:

[0032] In the process of cultivating baby snails, the present invention co-cultivates seahorses in the cultivation water body, and the seahorses can use their straw-shaped snouts to suck tiny plankton, such as sword water fleas, etc. The use of seahorses for biological control can accurately and effectively suppress the number of sword water fleas, reduce their harm to baby snail cultivation, and thus ensure the healthy growth, survival rate and high standards of baby snails. Long-stem grape fern algae are planted in the sand layer for cultivating baby snails to provide a natural hiding place for seahorses, and can also absorb residual N, P and other elements in the water body, thereby playing a role in purifying water quality.

[0033] In addition, the inventors also found through research that the aquaculture ecosystem formed by the baby snails, seahorses and long-stem grape fern also has a certain balance and stability, and the mixed culture of seahorses and the planting of long-stem grape fern will not harm the aquaculture ecosystem of the baby snails.

[0034] Furthermore, the inventors have found through research that the composite pharmaceutical composition used in the present invention also has an excellent promoting effect on the system of long-stemmed grape fern algae and mixed culture of seahorse. On the one hand, the composite pharmaceutical composition can also provide sufficient and necessary key nutrients for the long-stemmed grape fern algae, promote its cell division and growth, make its algae more luxuriant, and thus achieve rapid growth and reproduction.

[0035] On the other hand, the composite pharmaceutical composition also has the following effects on seahorses: (1) improving the survival rate, by enhancing the seahorse's immunity and ability to resist diseases, reducing the risk of disease and death, thereby improving its survival rate. (2) promoting growth and development, helping to optimize the seahorse's nutrient absorption and metabolism, accelerating its growth rate, and thus increasing production. (3) enhancing the ability to adapt to the environment, so that the seahorse can better adapt to changes in the breeding environment, such as fluctuations in water quality and temperature.

[0036] The present invention provides an eco-friendly and sustainable way to deal with the outbreak of sword water fleas, reducing the potential pollution to the breeding environment and the adverse effects on the baby snails. This biological control-based strategy is conducive to improving the economic benefits and sustainability of large-scale production of baby snails, and provides strong support and guarantee for the green development of the breeding industry.

[0037] Preferably, during the culture of Babylonia snails, the water temperature of the culture water is maintained at 24-28°C, the dissolved oxygen is 5-6 mg / L, and the salinity is 26-30.

[0038] Preferably, the compound pharmaceutical composition is added in a manner selected from at least one of the following (a) to (d):

[0039] (a) The dosage of the composite pharmaceutical composition is 3 to 10 g / m 3 ;

[0040] (b) the compound pharmaceutical composition is used at an interval of adding to the entire pool every 10 to 15 days;

[0041] (c) stopping water injection before adding the pharmaceutical composition, stopping oxygen supply 5 to 10 minutes after splashing, continuing for 1 to 2 hours, and then changing the water;

[0042] (d) Use the combined pharmaceutical composition at night.

[0043] Preferably, when the compound pharmaceutical composition is used at night, a white light is turned on to illuminate the aquaculture water body to induce the sword water fleas in the aquaculture water body to gather in one place. More specifically, the area illuminated by the white light can account for 1 / 6 to 1 / 8 of the area of ​​the aquaculture pond.

[0044] Specifically, in the present invention, seahorses with clean body surface, no parasites and good vitality can be selected. More specifically, the seahorses can be Hippocampus erectus and / or Hippocampus abdominalis.

[0045] Specifically, the babylonia is of the genus Babylonia (Babylonia), including but not limited to Babylonia areolata, Babylonia lutosa, Babylonia formosae, etc.

[0046] Compared with the prior art, the present invention has the following beneficial effects:

[0047] The present invention provides a composite pharmaceutical composition, which has a significant effect on improving the survival rate and size of baby snails. On this basis, in the process of cultivating baby snails, the present invention plants long-stem grape fern algae and co-cultures seahorses, and adds the composite pharmaceutical composition to the cultivation water, thereby greatly reducing the number of sword water fleas in the process of cultivating baby snails, inhibiting the outbreak of sword water fleas, and further improving the survival rate and size of baby snails based on this.

[0048] Instruction Manual

[0049] Figure 1 Schematic diagram of the long-stemmed grape fern algae that has been cleaned and is ready for release.

[0050] Figure 2 and Figure 3 Schematic diagram of weighing the long-stem grape fern algae in advance according to the planting density and placing them in the culture pond.

[0051] Figure 4 This is a schematic diagram of the breeding pond for mixed culture of seahorses, long-stem grape fern algae and baby snails. DETAILED DESCRIPTION

[0052] The present invention is further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0053] The seabuckthorn flavonoids, berberine and salicylic acid used in the present invention are all purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0054] Example 1 Preparation of a composite pharmaceutical composition

[0055] According to the weight ratio, 10 parts of seabuckthorn flavonoids, 10 parts of berberine, 8 parts of salicylic acid, 2 parts of calcium hydrogen phosphate and 2 parts of potassium bicarbonate were selected respectively, and they were mixed evenly to prepare a composite pharmaceutical composition.

[0056] Example 2 Preparation of a composite pharmaceutical composition

[0057] According to weight proportions, 10 parts of seabuckthorn flavonoids, 10 parts of berberine, 10 parts of salicylic acid, 1 part of calcium hydrogen phosphate and 2 parts of potassium bicarbonate were selected respectively, and they were mixed evenly to prepare a composite pharmaceutical composition.

[0058] Example 3 Preparation of a composite pharmaceutical composition

[0059] According to the weight ratio, 9 parts of seabuckthorn flavonoids, 5 parts of berberine, 8 parts of salicylic acid, 2 parts of calcium hydrogen phosphate and 1 part of potassium bicarbonate were selected respectively, and they were mixed evenly to prepare a composite pharmaceutical composition.

[0060] Example 4 Preparation of a composite pharmaceutical composition

[0061] According to the weight proportions, 8 parts of seabuckthorn flavonoids, 7 parts of berberine, 6 parts of salicylic acid, 2 parts of calcium hydrogen phosphate and 2 parts of potassium bicarbonate were selected respectively, and they were mixed evenly to prepare a composite pharmaceutical composition.

[0062] Example 5: Breeding of Babylonia

[0063] The experiment used an indoor cement pool of 3m×5m×0.5m as the breeding pool for Babylonia areolata. Seawater from the open sea was pumped into the filtration pool through a sand filter well and then disinfected with ultraviolet light before entering the breeding pool. Juvenile Babylonia areolata snails with a shell length of 10 to 15 mm were placed in the pool, and the breeding density in each pool was 800 snails / m 2 During the breeding period, the water in the breeding pond was controlled at a water temperature of 24-28°C, a dissolved oxygen of 5-6 mg / L, and a salinity of 26-30. After feeding the bait barramundi in the afternoon, the water in the breeding pond was changed, and the composite pharmaceutical composition prepared in Example 1 was sprayed throughout the pond once every 12 days, with a dosage of 8 g / m 3 (Based on the volume of the aquaculture water body). Stop water injection before splashing, stop oxygen supply 5 minutes after splashing, and continue this state for 1 hour, then open the water valve to change the water. The composite drug composition is used at night. The growth status of the snails in the aquaculture pond needs to be observed every day. If dead individuals or clusters are found, they should be cleaned out in time.

[0064] Example 6: Cultivation of Babylonia

[0065] The difference between this embodiment and embodiment 5 is that the compound pharmaceutical composition is prepared by adopting embodiment 2.

[0066] Example 7: Breeding of Babylonia

[0067] The difference between this embodiment and embodiment 5 is that the compound pharmaceutical composition is prepared by adopting embodiment 3.

[0068] Example 8 A method for controlling the outbreak of sword water fleas in large-scale production of Babylonia

[0069] The experiment used an indoor cement pool of 3m×5m×0.5m as the breeding pool for Babylonia areolata. Seawater from the open sea was pumped into the filtration pool through a sand filter well and then disinfected with ultraviolet light before entering the breeding pool. Juvenile Babylonia areolata snails with a shell length of 10 to 15 mm were placed in the pool, and the breeding density in each pool was 800 snails / m 2 . Plant long-stem grape fern algae on the culture sand at a density of 0.2kg / m 2 The culture pond is mixed with 1cm long seahorses at a density of 6 / m 2 During the breeding period, the water in the breeding pond was controlled at a water temperature of 24-28°C, a dissolved oxygen of 5-6 mg / L, and a salinity of 26-30. After feeding the bait barramundi in the afternoon, the water in the breeding pond was changed, and the composite pharmaceutical composition prepared in Example 1 was sprayed throughout the pond once every 12 days, with a dosage of 8 g / m 3 (Based on the volume of the aquaculture water body). Stop water injection before splashing, stop oxygen supply 5 minutes after splashing, and this state lasts for 1 hour, then open the water valve to change the water. The composite pharmaceutical composition is used at night. At this time, the white light above the aquaculture pond needs to be turned on to lure the sword water fleas on the surface of the aquaculture pond to gather in one place, and the irradiation area accounts for about 1 / 8 of the area of ​​the aquaculture pond. The growth status of the wind snails, seahorses and long-stem grape fern algae in the aquaculture pond needs to be observed every day. If dead individuals or clusters are found, they should be cleaned out in time.

[0070] Example 9 A method for controlling the outbreak of sword fleas in large-scale production of Babylonia

[0071] The difference between this embodiment and embodiment 8 is that the compound pharmaceutical composition is prepared by adopting embodiment 2.

[0072] Example 10 A method for controlling the outbreak of sword water fleas in large-scale production of Babylonia

[0073] The difference between this embodiment and embodiment 8 is that the compound pharmaceutical composition is prepared by adopting embodiment 3.

[0074] Comparative Example 1

[0075] The difference between this comparative example and Example 5 is that Example 1 was not used to prepare the composite pharmaceutical composition.

[0076] Comparative Example 2

[0077] The difference between this comparative example and Example 5 is that the composite pharmaceutical composition in Example 1 contains only seabuckthorn flavonoids and berberine.

[0078] Comparative Example 3

[0079] The difference between this comparative example and Example 5 is that the composite pharmaceutical composition in Example 1 contains only seabuckthorn flavonoids and salicylic acid.

[0080] Comparative Example 4

[0081] The difference between this comparative example and Example 5 is that the composite pharmaceutical composition in Example 1 contains only berberine and salicylic acid.

[0082] Comparative Example 5

[0083] The difference between this comparative example and Example 5 is that the composite pharmaceutical composition in Example 1 contains only berberine.

[0084] Comparative Example 6

[0085] The difference between this comparative example and Example 5 is that the compound pharmaceutical composition in Example 1 contains only salicylic acid.

[0086] Comparative Example 7

[0087] The difference between this comparative example and Example 8 is that Example 1 was not used to prepare the composite pharmaceutical composition.

[0088] Comparative Example 8

[0089] The difference between this comparative example and Example 8 is that: Example 1 was not used to prepare the composite pharmaceutical composition; and seahorses were not co-cultured.

[0090] Comparative Example 9

[0091] The difference between this comparative example and Example 8 is that: Example 1 was not used to prepare the composite pharmaceutical composition; and long-stem grape fern algae was not planted.

[0092] Test Case

[0093] The breeding cycle is 2 months. The density of sword water fleas in the breeding pond was tested once at the beginning of breeding and once every 2 months. Randomly draw 3 times, 1L of water samples from the breeding pond each time, filter them with a 30μm zooplankton filter, carry out sampling of zooplankton in the water, and conduct tests. Invert the filter, rinse it with distilled water, obtain the water sample containing zooplankton, bring the water sample into the laboratory, concentrate it to 50mL, add an appropriate amount of formaldehyde (formaldehyde content of 4%) to fix the zooplankton, and stir it evenly with the help of a glass rod. Let it stand for 1 minute, pour out the excess water in the upper layer, transfer the remaining water sample to a culture dish, observe and count the number of sword water flea adults and larvae under a microscope, and make corresponding records. Observe and count continuously for 3 times, and regard the average value as the final measurement value. Pick out the dead snails every day and make a record, and record the number of dead Dongfeng snails.

[0094] Snail survival rate = (total number of seedlings released - number of dead snails) / total number of seedlings released × 100%.

[0095] Snail specifications: Randomly take 1 kg of Babylonia areolata from the breeding pond three times, count the numbers, and the average value is the size of Babylonia areolata (pieces / kg).

[0096] Seahorse production: Dead seahorses were picked out and recorded every day.

[0097] Yield of long-stem grape fern algae: collect the long-stem grape fern algae after 2 months of cultivation, drain and weigh (kg).

[0098] The above statistical results are shown in Table 1 below.

[0099] Table 1

[0100]

[0101]

[0102] It can be seen from the above-mentioned Examples 5 to 7 that the composite pharmaceutical composition provided by the present invention can significantly improve the survival rate and snail size of the Babylonia snail. The survival rate of the Babylonia snail is ≥84.4%; the size of the Babylonia snail is within 441 pieces / kg. Furthermore, it can be seen from the above-mentioned Examples 8 to 10 that the composite pharmaceutical composition provided by the present invention can significantly improve the survival rate and snail size of the Babylonia snail in the process of Babylonia snail cultivation, while reducing the density of sword water fleas in the breeding water. The survival rate of the Babylonia snail is ≥95.5%; the size of the Babylonia snail is within 438 pieces / kg, and the density of sword water fleas is ≤13 pieces / L.

[0103] It can be seen from Example 5, Comparative Example 1, and Comparative Examples 2 to 6 that the combined use of seabuckthorn flavonoids, berberine, and salicylic acid has the effect of synergistically promoting the growth and development of Babylonia snails and improving the survival rate of Babylonia snails. However, it is difficult to achieve the technical effect of the present invention by using one or two of the components alone.

[0104] It can be seen from Example 8, Comparative Example 1 and Comparative Example 7 that it is difficult to achieve the technical effect of the present invention by only planting long-stem grape fern algae and mixed breeding of seahorses during the breeding process. The survival rate and specifications of the snails in Comparative Example 1 and Comparative Example 7 are significantly lower than those in Example 8, and the seahorse yield and algae yield are significantly lower than those in the embodiment.

[0105] The foregoing examples are merely illustrative and are used to explain some features of the method of the present invention. The appended claims are intended to require the widest possible range that can be imagined, and the embodiments presented herein are demonstrated by the applicant's actual test results. Therefore, the applicant's intention is that the appended claims are not limited by the selection of examples that illustrate the features of the present invention. Some numerical ranges used in the claims also include sub-ranges therein, and changes in these ranges should also be interpreted as being covered by the appended claims where possible.

Claims

1. The use of a composite pharmaceutical composition in reducing the number of sword water fleas, characterized in that: The compound pharmaceutical composition comprises, by weight: 5 to 10 parts of seabuckthorn flavonoids, 5 to 10 parts of berberine and 6 to 12 parts of salicylic acid.

2. A method for controlling a cyclops outbreak in a breeding pond, characterized in that: Long-stem grape fern algae are planted and seahorses are mixed and a compound pharmaceutical composition is added to the aquaculture water; the compound pharmaceutical composition comprises, by weight, 5 to 10 parts of seabuckthorn flavonoids, 5 to 10 parts of berberine and 6 to 12 parts of salicylic acid.

3. The method according to claim 2, characterized in that: The planting density of long-stem grape fern is 0.1~0.3kg / m 2 .

4. The method according to claim 2, characterized in that: The breeding density of seahorses is 5 to 10 / m 2 .

5. The method according to claim 2, characterized in that: Dongfeng snails are also cultured in the breeding ponds.

6. The method according to claim 2 or 5, characterized in that: During the breeding process, the water temperature of the breeding water is maintained at 24-28°C, the dissolved oxygen is 5-6 mg / L, and the salinity is 26-30.

7. The method according to claim 2, characterized in that: The compound pharmaceutical composition is added in a manner selected from at least one of the following (a) to (d): (a) The dosage of the composite pharmaceutical composition is 3 to 10 g / m 3 ; (b) the compound pharmaceutical composition is used at an interval of adding to the entire pool every 10 to 15 days; (c) stopping water injection before adding the pharmaceutical composition, stopping oxygen supply 5 to 10 minutes after splashing, continuing for 1 to 2 hours, and then changing the water; (d) Use the combined pharmaceutical composition at night.

8. The method according to claim 7, characterized in that: When the compound pharmaceutical composition is used at night, a white light is turned on to illuminate the aquaculture water body to induce the sword water fleas in the aquaculture water body to gather in one place.

9. The method according to claim 2, characterized in that: The compound pharmaceutical composition further comprises: 1 to 2 parts of calcium additives and / or 1 to 2 parts of potassium additives.

10. The method according to claim 9, characterized in that: The calcium additive is selected from one or more of calcium carbonate, calcium phosphate, calcium gluconate, and calcium chloride; the potassium additive is selected from one or more of potassium chloride, potassium carbonate, potassium dihydrogen phosphate, and dipotassium hydrogen phosphate.

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