Preparation method of biopesticide preparation for preventing and treating mulberry virus diseases

Abstract

The invention discloses a preparation method of a biological pesticide preparation for preventing and treating mulberry virus diseases, and the preparation method is applied to the technical field of preparation of biological pesticide preparations. The composition has a remarkable inhibition effect on various common mulberry virus diseases, can effectively reduce the morbidity of the mulberry virus diseases and improve the health condition and yield of mulberries, and has good stability in the storage and use processes by adding the stabilizer and optimizing the preparation process, the effective components are not easy to inactivate, and the preparation is safe and reliable. The control effect can be kept for a long time, plant extracts and microorganisms adopted by the invention are natural sources, and compared with chemical pesticides, the influence on the environment and the health of silkworms is extremely small, and the development requirements of green agriculture are met.

Description

Technical Field

[0001] The invention belongs to the technical field of biological pesticide preparation, and particularly relates to a method for preparing a biological pesticide preparation for preventing and treating mulberry virus diseases. Background Art

[0002] As an important economic crop, mulberry plays a key role in the sericulture industry. However, the frequent occurrence of mulberry virus diseases has seriously affected the growth and yield of mulberry trees, causing huge losses to the sericulture industry.

[0003] At present, chemical pesticides are used to control mulberry virus diseases. Although chemical pesticides can control the spread of mulberry virus diseases to a certain extent, they have many disadvantages. On the one hand, long-term and large-scale use of chemical pesticides can easily lead to drug resistance in mulberry trees, which gradually reduces the subsequent control effect. On the other hand, the residual problem of chemical pesticides seriously threatens the health of silkworms and the quality of silk.

[0004] The use of traditional biological control methods, such as utilizing the natural inhibitory effects of certain natural enemy insects or microorganisms to control mulberry virus diseases, although it has the advantage of being environmentally friendly, has the problem of unstable control effects. Since the population size and activity range of natural enemy insects are greatly affected by environmental factors, it is difficult to accurately control their inhibitory effects on mulberry virus diseases. For microbial control, relying solely on the inhibitory effects of microorganisms existing in nature on mulberry virus diseases is often limited and cannot meet the needs of large-scale mulberry planting areas for efficient control.

[0005] Combining the above two problem entry points, the existing technical means for preventing and controlling mulberry virus diseases have defects to varying degrees. Therefore, we proposed a method for preparing a biological pesticide formulation for preventing and controlling mulberry virus diseases that is efficient, stable, environmentally friendly and cost-effective. Summary of the invention

[0006] The purpose of the present invention is to propose a preparation method of a biological pesticide preparation for preventing and treating mulberry virus diseases. The method has the advantages of selecting plant extracts with antiviral and plant immunity enhancing effects and synergistic effects with microbial fermentation broth, having a significant inhibitory effect on a variety of common mulberry virus diseases, being able to effectively reduce the incidence of mulberry virus diseases, and improving the health and yield of mulberry trees. Moreover, by adding stabilizers and optimizing the preparation process, the preparation has good stability during storage and use, the effective ingredients are not easy to inactivate, and the prevention and control effect can be maintained for a long time. Moreover, the plant extracts and microorganisms used in the present invention are of natural origin. Compared with chemical pesticides, the impact on the environment and the health of silkworms is extremely small, which meets the development requirements of green agriculture.

[0007] The above technical purpose of the present invention is achieved through the following technical scheme: a method for preparing a biological pesticide preparation for preventing and controlling mulberry virus disease, comprising the following steps:

[0008] S1. Material preparation: Select a variety of plants with antiviral activity, including the roots, stems, and leaves of Radix Isatidis, Radix Sophorae Flavescentis, Radix Astragali, and Portulaca Oleracea; select Bacillus spores or Trichoderma microbial strains; prepare auxiliary materials, including distilled water, organic solvents, surfactants, and stabilizers;

[0009] S2. Preparation of plant extracts: The selected plant material is crushed into powder, and the crushed plant powder and an organic solvent are placed in an extraction container, and the solvent extraction method is used for immersion extraction;

[0010] The mixed solution after extraction is filtered to obtain a filtrate, and then concentrated under reduced pressure to obtain a concentrated plant extract;

[0011] S3. Preparation of microbial fermentation broth: inoculating the selected microbial strains into the culture medium for activation culture, and then inoculating into the fermentation medium for fermentation culture after activation culture;

[0012] After the fermentation is completed, the fermentation liquid is subjected to centrifugal filtration to remove the bacteria and impurities therein to obtain a pure microbial fermentation liquid;

[0013] S4. Preparation of biological agricultural preparations: The concentrated plant extract and the microbial fermentation broth are mixed, and distilled water is added to obtain a mixed solution, and then a surfactant and a stabilizer are added to the mixed solution, and the mixture is mixed uniformly to obtain a uniform biological pesticide preparation;

[0014] S5. Quality monitoring: The prepared biopesticide preparations are subjected to quality inspections, including inspections of indicators such as active ingredient content, pH value, stability, and toxicity. If they meet the inspection requirements, the biopesticide preparations for the prevention and treatment of mulberry virus diseases are obtained.

[0015] The present invention is further configured as follows: the organic solvent selected in step S1 is ethanol, and the amount of ethanol added should ensure that the solvent covers the plant powder.

[0016] The present invention is further configured as follows: the Bacillus or Trichoderma selected in step S1 can be obtained from a professional microbial strain library or separated from an environment such as healthy mulberry root soil.

[0017] The present invention is further configured as follows: in step S2, the microbial strains are activated and cultured under the conditions of a temperature of 30-37° C. and a humidity of 60-80%, and the activation time is 12-24 hours.

[0018] The present invention is further configured as follows: the fermentation medium is prepared by mixing a carbon source, a nitrogen source, an inorganic salt, etc., and the carbon source and the nitrogen source are glucose and yeast powder respectively.

[0019] The present invention is further configured as follows: the fermentation time in step S2 is 2-5 days, the fermentation temperature is 25-40° C., and the pH value is 6.5-7.5.

[0020] The present invention is further configured such that the ratio of the plant extract to the microbial fermentation liquid in step S3 is 1:1.

[0021] The present invention is further configured as follows: the amount of the surfactant added in step S3 is 0.5-2% of the total weight of the preparation, and the amount of the stabilizer added is 0.1-1%.

[0022] The present invention is further configured as follows: the surfactant is Tween-80, and the stabilizer is xanthan gum.

[0023] The present invention is further configured such that the particle size of the plant powder after the crushing in step S2 is between 10 and 50 microns.

[0024] In summary, the present invention has the following beneficial effects:

[0025] By selecting plant extracts with antiviral and plant immunity enhancing effects, and synergizing with microbial fermentation broth, the invention has a significant inhibitory effect on a variety of common mulberry virus diseases, can effectively reduce the incidence of mulberry virus diseases, and improve the health and yield of mulberry trees. Moreover, by adding stabilizers and optimizing the preparation process, the preparation has good stability during storage and use, the effective ingredients are not easily inactivated, and the prevention and control effects can be maintained for a long time. Moreover, the plant extracts and microorganisms used in the invention are of natural origin. Compared with chemical pesticides, they have little impact on the environment and the health of silkworms, and meet the development requirements of green agriculture. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The present invention is a schematic flow chart of a method for preparing a biological pesticide preparation for preventing and treating mulberry virus diseases. DETAILED DESCRIPTION

[0027] The present invention is further described in detail below in conjunction with the accompanying drawings.

[0028] Embodiment 1:

[0029] refer to Figure 1 , a method for preparing a biological pesticide preparation for preventing and controlling mulberry virus disease, comprising the following steps:

[0030] S1. Material preparation: Select a variety of plants with antiviral activity, including the roots, stems, and leaves of Radix Isatidis, Radix Sophorae Flavescentis, Radix Astragali, and Portulaca Oleracea; select Bacillus spores or Trichoderma microbial strains; prepare auxiliary materials, including distilled water, organic solvents, surfactants, and stabilizers;

[0031] S2. Preparation of plant extracts: The selected plant material is crushed into powder, and the crushed plant powder and an organic solvent are placed in an extraction container, and the solvent extraction method is used for immersion extraction;

[0032] The mixed solution after extraction is filtered to obtain a filtrate, and then concentrated under reduced pressure to obtain a concentrated plant extract;

[0033] S3. Preparation of microbial fermentation broth: inoculating the selected microbial strains into the culture medium for activation culture, and then inoculating into the fermentation medium for fermentation culture after activation culture;

[0034] After the fermentation is completed, the fermentation liquid is subjected to centrifugal filtration to remove the bacteria and impurities therein to obtain a pure microbial fermentation liquid;

[0035] S4. Preparation of biological agricultural preparations: The concentrated plant extract and the microbial fermentation broth are mixed, and distilled water is added to obtain a mixed solution, and then a surfactant and a stabilizer are added to the mixed solution, and the mixture is mixed uniformly to obtain a uniform biological pesticide preparation;

[0036] S5. Quality monitoring: The prepared biopesticide preparations are subjected to quality inspections, including inspections of indicators such as active ingredient content, pH value, stability, and toxicity. If they meet the inspection requirements, the biopesticide preparations for the prevention and treatment of mulberry virus diseases are obtained.

[0037] Embodiment 2:

[0038] The organic solvent selected in step S1 is ethanol, and the amount of ethanol added should ensure that the solvent covers the plant powder.

[0039] The Bacillus or Trichoderma selected in step S1 can be obtained from a professional microbial strain library or isolated from an environment such as soil at the root of a healthy mulberry tree.

[0040] In step S2, the microbial strains are activated and cultured at a temperature of 30-37° C. and a humidity of 60-80%, and the activation time is 12-24 hours.

[0041] The fermentation medium is prepared by mixing a carbon source, a nitrogen source and inorganic salts, and the carbon source and the nitrogen source are glucose and yeast powder respectively.

[0042] The fermentation time in step S2 is 2-5 days, the fermentation temperature is 25-40° C., and the pH value is 6.5-7.5.

[0043] The ratio of the plant extract to the microbial fermentation broth in step S3 is 1:1.

[0044] The amount of surfactant added in step S3 is 0.5-2% of the total weight of the preparation, and the amount of stabilizer added is 0.1-1%.

[0045] The surfactant is Tween-80 and the stabilizer is xanthan gum.

[0046] The particle size of the plant powder after crushing in step S2 is between 10 and 50 microns.

[0047] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. A method for preparing a biological pesticide preparation for preventing and treating mulberry virus diseases, characterized in that: The following steps are involved: S1. Material preparation: Select a variety of plants with antiviral activity, including the roots, stems, and leaves of Radix Isatidis, Radix Sophorae Flavescentis, Radix Astragali, and Portulaca Oleracea; select Bacillus spores or Trichoderma microbial strains; prepare auxiliary materials, including distilled water, organic solvents, surfactants, and stabilizers; S2. Preparation of plant extracts: The selected plant material is crushed into powder, and the crushed plant powder and an organic solvent are placed in an extraction container, and the solvent extraction method is used for immersion extraction; The mixed solution after extraction is filtered to obtain a filtrate, and then concentrated under reduced pressure to obtain a concentrated plant extract; S3. Preparation of microbial fermentation broth: inoculating the selected microbial strains into the culture medium for activation culture, and then inoculating into the fermentation medium for fermentation culture after activation culture; After the fermentation is completed, the fermentation liquid is subjected to centrifugal filtration to remove the bacteria and impurities therein to obtain a pure microbial fermentation liquid; S4. Preparation of biological agricultural preparations: The concentrated plant extract and the microbial fermentation broth are mixed, and distilled water is added to obtain a mixed solution, and then a surfactant and a stabilizer are added to the mixed solution, and the mixture is mixed uniformly to obtain a uniform biological pesticide preparation; S5. Quality monitoring: The prepared biopesticide preparations are subjected to quality inspections, including inspections of indicators such as active ingredient content, pH value, stability, and toxicity. If they meet the inspection requirements, the biopesticide preparations for the prevention and treatment of mulberry virus diseases are obtained.

2. The method for preparing a biological pesticide preparation for preventing and treating mulberry virus disease according to claim 1, characterized in that: The organic solvent selected in step S1 is ethanol, and the amount of ethanol added should ensure that the solvent covers the plant powder.

3. The method for preparing a biological pesticide preparation for preventing and treating mulberry virus disease according to claim 1, characterized in that: The Bacillus or Trichoderma selected in step S1 can be obtained from a professional microbial strain library or isolated from an environment such as healthy mulberry root soil.

4. The method for preparing a biological pesticide preparation for preventing and treating mulberry virus disease according to claim 1, characterized in that: In step S2, the microbial strains are activated and cultured under the conditions of a temperature of 30-37° C. and a humidity of 60-80%, and the activation time is 12-24 hours.

5. The method for preparing a biological pesticide preparation for preventing and treating mulberry virus diseases according to claim 1, characterized in that: The fermentation medium is prepared by mixing a carbon source, a nitrogen source, an inorganic salt, etc., and the carbon source and the nitrogen source are glucose and yeast powder respectively.

6. The method for preparing a biological pesticide preparation for preventing and treating mulberry virus diseases according to claim 1, characterized in that: The fermentation time in step S2 is 2-5 days, the fermentation temperature is 25-40° C., and the pH value is 6.5-7.

5.

7. The method for preparing a biological pesticide preparation for preventing and treating mulberry virus diseases according to claim 1, characterized in that: The ratio of the plant extract to the microbial fermentation broth in step S3 is 1:

1.

8. The method for preparing a biological pesticide preparation for preventing and treating mulberry virus diseases according to claim 1, characterized in that: The amount of surfactant added in step S3 is 0.5-2% of the total weight of the preparation, and the amount of stabilizer added is 0.1-1%.

9. The method for preparing a biological pesticide preparation for preventing and controlling mulberry virus diseases according to claim 1, characterized in that: The surfactant is Tween-80, and the stabilizer is xanthan gum.

10. The method for preparing a biological pesticide preparation for preventing and controlling mulberry virus diseases according to claim 1, characterized in that: The particle size of the plant powder after crushing in step S2 is between 10 and 50 microns.

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