Bacteriostatic pesticide composition and preparation method thereof

Abstract

The invention relates to a bacteriostatic pesticide composition and a preparation method thereof. The bacteriostatic pesticide composition comprises a concentrated solution extracted from croton, chinaberry seed and bacteriostatic traditional Chinese medicine primer in a mass ratio of 1: (1-3): (0.1-0.5). The raw materials of the pesticide composition are all pure traditional Chinese medicines from the nature, the pesticide composition can be naturally degraded, has no toxicity to soil, underground water and the like, is safe to use, has an insecticidal effect of a pesticide, also has an antibacterial function due to introduction of antibacterial traditional Chinese medicines, and has a good market application prospect.

Description

Technical Field

[0001] The invention belongs to the field of pesticides, and particularly relates to an antibacterial pesticide composition and a preparation method thereof. Background Art

[0002] The use of chemical pesticides on food crops and cash crops is to eliminate or reduce pests and diseases and increase crop yields. For more than half a century, people have become accustomed to the use of chemical synthetic pesticides. Chemical synthetic pesticides are widely used worldwide because of their good effects, quick results, low prices and ease of use. However, the use of chemical synthetic pesticides has also brought many well-known problems: food, soil, groundwater, rivers, lakes, oceans and air are all seriously polluted by chemical residues, and have side effects on non-target insects and other organisms, leading to the destruction of the ecosystem. At the same time, the number and variety of insects that have developed resistance and recovery capabilities to chemical synthetic pesticides are also increasing. In addition, the use, abuse and improper handling of highly toxic chemical synthetic pesticides have also led to many frequent poisoning incidents. Therefore, it is necessary to develop a more environmentally friendly pesticide. Summary of the invention

[0003] The technical problem to be solved by the present invention is to provide an antibacterial pesticide composition and a preparation method thereof. The raw materials of the pesticide composition are all pure traditional Chinese medicines from nature, which can be naturally degraded, are non-toxic to the environment, and are safe to use. While having the effects of the pesticide itself, due to the introduction of antibacterial traditional Chinese medicine lead, it also has an antibacterial function and has good market application prospects.

[0004] The invention provides an antibacterial pesticide composition, which comprises a concentrated solution extracted from croton seeds, chinaberry seeds and antibacterial traditional Chinese medicine lead in a mass ratio of 1:1-3:0.1-0.5.

[0005] Preferably, the antibacterial Chinese herbal medicine lead includes green leaves, honeysuckle, coptis root, gallnut or other traditional antibacterial Chinese medicines.

[0006] Preferably, the volume percentage of the concentrate in the composition is 65-75%.

[0007] Preferably, the composition further comprises dimethyl sulfoxide, limonene, a surfactant and a vegetable oil emulsifier.

[0008] More preferably, the volume percentage of dimethyl sulfoxide in the composition is 5-15%.

[0009] More preferably, the volume percentage of limonene in the composition is 1-10%.

[0010] More preferably, the volume percentage of the defoaming agent in the composition is 0.1-0.5%.

[0011] More preferably, the surfactant is a silicone surfactant, and its volume percentage in the composition is 5-15%.

[0012] More preferably, the vegetable oil emulsifier is 602, and its volume percentage in the composition is 0.1-1%.

[0013] The present invention also provides a method for preparing an antibacterial pesticide composition, comprising the following steps:

[0014] (1) crushing croton and chinaberry, and then mixing them with antibacterial Chinese medicine; after stirring evenly, adding them to a continuous extraction device, firstly soaking them with alcohol, then synchronously extracting them with ultrasonic waves, and simultaneously discharging and recovering the drug residues;

[0015] (2) filtering the liquid produced by the extraction to obtain a clean liquid; performing vacuum concentration to recover the alcohol;

[0016] (3) The concentrated pesticide solution is mixed with dimethyl sulfoxide, limonene, a surfactant and a vegetable oil emulsifier in a temporary storage tank for emulsification to obtain an antibacterial pesticide composition for filling.

[0017] The alcohol concentration in steps (1) and (2) is 60-75%.

[0018] The ultrasonic wave in step (1) is carried out at room temperature, the ultrasonic wave wavelength is 25-30 Hz, and the power is 1000w-2000w.

[0019] The filtration in step (2) is performed using 200-mesh microporous filtration.

[0020] The temperature of the vacuum concentration in step (2) is 25-40°C.

[0021] The invention also provides an application of the antibacterial pesticide composition in pesticides.

[0022] Beneficial Effects

[0023] (1) The raw materials of the pesticide composition of the present invention are all pure Chinese medicines, which are naturally degradable, non-toxic to soil, groundwater, etc., and safe to use. In addition to having the insecticidal effect of the pesticide itself, due to the introduction of antibacterial Chinese medicine lead, it also has an antibacterial function, and has a good market application prospect;

[0024] (2) The addition of the antibacterial Chinese herbal medicine in the present invention also synergistically enhances the anthelmintic and antibacterial effects of azadirachtin and croton; (3) The preparation process of the present invention is a continuous extraction automation process, and the Chinese herbal medicine raw materials do not need to be extracted separately in advance, but are uniformly added to the raw material infiltration and ultrasonic extraction integrated tank for compound extraction according to the traditional Chinese medicine principle of "monarch, minister, assistant and envoy"; at the same time, the extraction solvents used in the present invention are all alcohol, and no toxic solvents such as petroleum ether are used, which is environmentally friendly. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The present invention is a schematic diagram of the preparation process of the composition.

[0026] Figure 2 This is the inhibitory effect of ZWY-1 on Botrytis cinerea.

[0027] Figure 3 This is the inhibitory effect of ZWY-2 on Botrytis cinerea.

[0028] Figure 4 This is the inhibitory effect of ZWY-1 on Verticillium dahliae.

[0029] Figure 5 This is the inhibitory effect of ZWY-2 on Verticillium dahliae.

[0030] Figure 6 This is the inhibitory effect of ZWY-1 on Fusarium wilt.

[0031] Figure 7 This is the inhibitory effect of ZWY-2 on Fusarium wilt. DETAILED DESCRIPTION

[0032] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms fall within the scope limited by the appended claims of the application equally.

[0033] Example 1

[0034] This embodiment provides an antibacterial pesticide composition, which includes 41 kg of Croton tiglium, 123 kg of Toosendan fruit and 9.1 kg of green leaf extract. The volume of the concentrate is 141.1L.

[0035] The composition also includes 15.84 L of dimethyl sulfoxide, 9.1 L of limonene, 29.8 L of an organic silicon surfactant, and 1.2 L of a vegetable oil emulsifier 602.

[0036] like Figure 1As shown, this embodiment also provides a method for preparing an antibacterial pesticide composition, comprising the following steps:

[0037] (1) Crush croton and neem seeds to 80 mesh, mix them in a certain proportion, and then mix them into the crushed green leaves; after stirring evenly, add them into a continuous extraction device, first soak them with 75% alcohol, then perform ultrasonic extraction simultaneously, and discharge and recover the residues, and enter a concentration device; wherein the ultrasonic temperature is 25-45°C, the ultrasonic wavelength is 25Hz, and the power is 1000w;

[0038] (2) filtering the drug solution produced by the extraction to obtain a drug solution; performing vacuum concentration to recover the alcohol; wherein the filtration is performed by a 200 μm microporous filtration; and the temperature of the vacuum concentration is 25° C. to 45° C.;

[0039] (3) The concentrated pesticide solution is mixed with dimethyl sulfoxide, limonene, a surfactant and a vegetable oil emulsifier in a temporary storage tank for emulsification to obtain an antibacterial pesticide composition for filling.

[0040] Comparative Example 1

[0041] The difference from Example 1 is that 9.1 kg of green leaves were not added.

[0042] Pest control trend experiment:

[0043] Table 1 Fruit fly experiment of packaging line products 2023.4.25 (Comparative Example 1)

[0044]

[0045] (The figures in the table are the number of fruit flies that died and the percentage of death)

[0046] Table 2 Packaging line product fruit fly experiment 2023.11.8 (Example 1)

[0047]

[0048]

[0049] (The figures in the table are the number of fruit flies that died and the percentage of death)

[0050] Antibacterial experiment:

[0051] 1.1 Test name

[0052] The indoor toxicity of the botanical pesticide with added drug inducer (ie, Example 1, denoted as ZWY-2) and the stock botanical pesticide without added drug inducer (ie, Comparative Example 1, denoted as ZWY-1) to Fusarium, Pseudomonas aeruginosa, and Botrytis cinerea.

[0053] 1.2 Purpose of the experiment

[0054] The test evaluates the indoor toxicity of the developed botanical pesticides (ZWY-1 and ZWY-2) against Fusarium wilt, Trichosporon wilt, and Botrytis cinerea, providing a basis for the scientific use of this product.

[0055] 1.3 Test strains

[0056] Fusarium, Neopestalotiopsis and Botrytis were all provided by the Provincial Key Laboratory of Pesticide Toxicology and Application Technology of Shandong Agricultural University and activated with PDA medium for later use.

[0057] 1.3 Test methods

[0058] Growth rate method: In an ultra-clean workbench, two kinds of botanical pesticides were gradiently diluted with sterile water to obtain 250×, 500×, 1000×, 2000× and 4000× solutions. Use a pipette to take 1mL of each concentration of solution, add 49mL of sterilized PDA culture medium cooled to 50-60°C, mix thoroughly and prepare 3 drug-containing PDA plates. After the plate solidifies, use a sterile punch with an inner diameter of 7mm to punch a bacterial cake from the edge of the activated pathogen, inoculate it in the center of the drug-containing PDA plate, and use sterile water instead of the solution as a control. Culture in the dark at 25°C. When the colony of the control group grows to about 2 / 3 of the culture dish, the inhibition rate of each concentration of solution treatment is determined by the cross method.

[0059] Inhibition rate (%) = (colony diameter of control group - colony diameter of treatment group) / (colony diameter of control group - diameter of bacterial cake) × 100.

[0060] 2 Test results

[0061] 2.12 plant-derived agents at different concentrations for inhibiting Fusarium

[0062] Table 3 Inhibitory effects of 2 plant-derived agents on Fusarium

[0063]

[0064]

[0065] From Table 3, Figure 2 , Figure 3 It can be seen that both plant-derived pesticides have a certain inhibitory effect on the mycelial growth of Fusarium, among which ZWY-2 has a higher inhibitory effect than ZWY-1. The mycelial growth inhibition rates of the two agents at 250 times dilution were 11.97% and 19.32%, respectively.

[0066] 2.2 Inhibitory effects of different concentrations of 22 botanical pesticides on Trichosporon spp.

[0067] Table 4 The inhibitory effect of 2 kinds of plant-derived agents on Trichosporon pseudodiscus

[0068]

[0069] From Table 4, Figure 4 , Figure 5 It can be seen that the two botanical pesticides have high inhibitory effects on Trichosporon spp. (the pathogen of Verticillium wilt), among which ZWY-2 has a higher inhibitory effect than ZWY-1. The 250-fold dilution of the two agents inhibited the mycelial growth of Trichosporon spp. by 42.47% and 55.90%, respectively. The 500-fold dilution of the two agents inhibited the mycelial growth of Trichosporon spp. by 39.44% and 49.80%, respectively. It can be seen that the botanical pesticide has certain application potential in the prevention and control of Verticillium wilt in crops.

[0070] 2.3 Inhibitory effects of different concentrations of two botanical pesticides on Botrytis cinerea

[0071] Table 5 Inhibitory effects of 2 plant-derived agents on Botrytis cinerea

[0072]

[0073] From Table 5, Figure 6 , Figure 7 It can be seen that the two botanical pesticides have a high inhibitory effect on Botrytis cinerea (the pathogen of gray mold), among which the inhibitory effect of ZWY-2 is much higher than that of ZWY-1. The 250-fold dilution of the two agents inhibited the mycelial growth of Botrytis cinerea by 27.68% and 44.03%, respectively. The 500-fold dilution of the two agents inhibited the mycelial growth of Botrytis cinerea by 25.22% and 39.40%, respectively.

[0074] in conclusion:

[0075] The botanical pesticides (with and without drug primers) developed have inhibitory effects on Fusarium, Trichosporon and Botrytis cinerea. The inhibitory effect of ZWY-2 with drug primers is slightly higher than that of ZWY-1 without drug primers. The inhibitory effect on Trichosporon is the highest, followed by Botrytis cinerea. It can be seen that the two botanical pesticides have a wide antibacterial spectrum and have a certain inhibitory effect on common pathogens in agricultural production. Among them, they have a high prevention and control potential for Verticillium wilt and gray mold.

Claims

1. An antibacterial pesticide composition, characterized in that: The antibacterial pesticide composition comprises a concentrated solution extracted from croton, chinaberry and antibacterial traditional Chinese medicine in a mass ratio of 1:1-3:0.1-0.

5.

2. The antibacterial pesticide composition according to claim 1, characterized in that: The volume percentage of the concentrate in the composition is 65-75%.

3. The antibacterial pesticide composition according to claim 1, characterized in that: The composition also includes dimethyl sulfoxide, limonene, a defoaming agent, a surfactant and a vegetable oil emulsifier.

4. The antibacterial pesticide composition according to claim 3, characterized in that: The volume percentage of the dimethyl sulfoxide in the composition is 5-15%.

5. The antibacterial pesticide composition according to claim 3, characterized in that: The volume percentage of the limonene in the composition is 1-10%.

6. The antibacterial pesticide composition according to claim 3, characterized in that: The surfactant is an organosilicon surfactant 284, and its volume percentage in the composition is 5-15%.

7. The antibacterial pesticide composition according to claim 3, characterized in that: The vegetable oil emulsifier is 602, and its volume percentage in the composition is 0.1-1%.

8. A method for preparing the antibacterial pesticide composition according to any one of claims 1 to 8, comprising the following steps: (1) crushing croton and neem seeds, and then mixing them with crushed antibacterial Chinese medicine lead; after stirring evenly, adding them to a continuous extraction device, firstly soaking them with alcohol, then synchronously extracting them with ultrasonic waves, and then discharging and recovering the drug residues; (2) filtering the product produced by the extraction, that is, the liquid medicine, to obtain a clean liquid medicine; performing vacuum concentration to recover the alcohol; (3) The concentrated pesticide solution is mixed with dimethyl sulfoxide, limonene, a surfactant and a vegetable oil emulsifier in a temporary storage tank for emulsification to obtain an antibacterial pesticide composition for automatic filling.

9. Use of the antibacterial pesticide composition according to any one of claims 1 to 8 in pesticides.

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