Feed additive for maintaining intestinal health of ducks and application of feed additive

Abstract

The invention relates to a feed additive for maintaining intestinal health of ducks and application of the feed additive. The feed additive comprises the following components: a Chinese herbal medicine fermentation product, tea tree oil, stachyose, honeycomb, seaweed gel and iodized salt. The novel duck feed additive is creatively developed, six components including the Chinese herbal medicine fermentation product, the tea tree oil, the stachyose, the honeycomb, the seaweed gel and the iodized salt are combined for use, and the tea tree oil has a relatively strong inhibition effect on harmful bacteria in intestinal tracts and reduces the probability of bacterial infection in the intestinal tracts; stachyose can promote proliferation of intestinal beneficial bacteria and improve the activity of the intestinal beneficial bacteria; the honeycomb element, the seaweed gel and the iodized salt have a synergistic effect, and have the effects of promoting intestinal mucosa repair and maintaining intestinal mucosa health. The formula is safe and non-toxic, all the components have a synergistic effect, and the feed has remarkable effects on improving the intestinal health of the ducks, reducing intestinal diseases of the ducks, improving the immunity of the ducks and improving the quality of duck eggs.

Description

Technical Field

[0001] The invention belongs to the technical field of poultry feed, and relates to a feed additive for maintaining the intestinal health of ducks and application thereof. Background Art

[0002] At present, intestinal health problems are common in duck farming, which not only affect the growth performance of ducks, but may also lead to diseases and deaths. In order to improve the intestinal health of ducks and reduce the incidence of diseases, antibiotics are often added to duck feed. However, long-term use of antibiotics will inhibit the normal function of immune cells in livestock and poultry, reduce disease resistance, and cause the accumulation of antibiotics in agricultural and livestock products, which will bring more harm to humans. Therefore, it is imperative to find new functional additives that can replace antibiotics.

[0003] At the same time, as people's demand for duck food increases, duck farming has developed rapidly, and the demand for ducklings has surged, which has put higher requirements on the production capacity of laying ducks and the quality of duck eggs. Summary of the invention

[0004] In view of the deficiencies of the prior art, the object of the present invention is to provide a feed additive for maintaining the intestinal health of ducks and its application.

[0005] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a feed additive for maintaining the intestinal health of ducks, wherein the feed additive comprises the following components: a fermented product of Chinese herbal medicine, tea tree oil, stachyose, honeycomb, alginate and iodized salt.

[0007] The present invention creatively develops a new feed additive for ducks, which combines six components, namely, Chinese herbal medicine fermentation products, tea tree oil, stachyose, honeycomb, seaweed and iodized salt. Tea tree oil has a strong inhibitory effect on harmful intestinal bacteria and reduces the probability of intestinal bacterial infection; stachyose can promote the proliferation of beneficial intestinal bacteria and improve the activity of beneficial intestinal bacteria; honeycomb, seaweed and iodized salt work together to promote intestinal mucosal repair and maintain the health of intestinal mucosa. The formula is safe and non-toxic, and the components work together to improve the intestinal health of ducks, reduce intestinal diseases of ducks, improve duck immunity and improve the quality of duck eggs.

[0008] Preferably, the fermentation substrate of the Chinese herbal medicine fermentation product includes alfalfa, Artemisia annua, Xanthium sibiricum, Polygonum hydropiper and mulberry leaf.

[0009] The fermentation substrate of the Chinese herbal fermentation product is more preferably a combination of five Chinese herbal medicines: alfalfa, artemisia annua, Xanthium sibiricum, Polygonum hydropiper and mulberry leaf, which cooperate with each other to synergistically enhance the intestinal health of ducks, reduce intestinal diseases of ducks, improve duck immunity and improve the quality of duck eggs.

[0010] Preferably, the fermentation bacteria of the Chinese herbal medicine fermentation product include Lactobacillus plantarum and Bifidobacterium.

[0011] The fermentation strains of the Chinese herbal medicine fermentation product are more preferably a combination of plant lactobacillus and bifidobacterium, which cooperate with each other to synergistically enhance the duck's intestinal health, reduce duck's intestinal diseases, improve duck's immunity, and improve duck egg quality.

[0012] Preferably, the ratio of the viable counts of Lactobacillus plantarum to Bifidobacterium is (1-3):1, for example 1:1, 2:3, 2:1, 5:2, 3:1, etc. Other specific point values ​​within this numerical range can be selected, and for the sake of brevity, they will not be described one by one here.

[0013] Preferably, the bifidobacterium is selected from any one of Bifidobacterium adolescentis, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve or Bifidobacterium bifidum, or a combination of at least two thereof; more preferably, a combination of Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum.

[0014] Among them, the bifidobacterium is more preferably a combination of Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum, and the fermentation effect is better when the ratio of the number of viable bacteria is (1-3):(1-3):1.

[0015] Preferably, the ratio of the viable counts of Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum is (1-3):(1-3):1, for example 1:1:1, 2:1:1, 3:1:1, 1:2:1, 2:2:1, 3:2:1, 1:3:1, 2:3:1, 3:3:1, etc. Other specific point values ​​within this numerical range can be selected. For the sake of brevity, they will not be described one by one here.

[0016] Preferably, the feed additives include, by mass, 3-7 parts of Chinese herbal fermentation products, 0.01-0.05 parts of tea tree oil, 1-3 parts of stachyose, 0.1-0.5 parts of honeycomb, 1-3 parts of alginate and 1-1.5 parts of iodized salt.

[0017] When the components are combined in the above-mentioned specific mass ratio, the prepared feed additive is more effective in maintaining intestinal health, improving the immunity of laying ducks and improving the quality of duck eggs.

[0018] The number of portions of the Chinese herbal fermentation product can be selected as 3 portions, 4 portions, 5 portions, 6 portions, 7 portions, etc.; the number of portions of the tea tree oil can be selected as 0.01 portions, 0.02 portions, 0.03 portions, 0.04 portions, 0.05 portions, etc.; the number of portions of the stachyose can be selected as 1 portion, 1.5 portions, 2 portions, 2.5 portions, 3 portions, etc.; the number of portions of the honeycomb can be selected as 0.1 portions, 0.2 portions, 0.3 portions, 0.4 portions, 0.5 portions, etc.; the number of portions of the alginate can be selected as 1 portion, 1.5 portions, 2 portions, 2.5 portions, 3 portions, etc.; the number of portions of the iodized salt can be selected as 1 portion, 1.1 portions, 1.2 portions, 1.3 portions, 1.4 portions, 1.5 portions, etc.; other specific point values ​​within the above numerical ranges can be selected, and for the sake of brevity, they will not be described one by one here.

[0019] Preferably, the fermentation substrate of the Chinese herbal fermentation product comprises, by weight, 3-5 parts of alfalfa, 1-3 parts of Artemisia annua, 1-3 parts of Xanthium sibiricum, 1-3 parts of Polygonum hydropiper and 0.5-1 part of mulberry leaf.

[0020] The number of portions of alfalfa can be selected from 3 portions, 3.5 portions, 4 portions, 4.5 portions, 5 portions, etc.; the number of portions of Artemisia annua can be selected from 1 portion, 1.5 portions, 2 portions, 2.5 portions, 3 portions, etc.; the number of portions of Xanthium sibiricum can be selected from 1 portion, 1.5 portions, 2 portions, 2.5 portions, 3 portions, etc.; the number of portions of Polygonum hydropiper can be selected from 1 portion, 1.5 portions, 2 portions, 2.5 portions, 3 portions, etc.; the number of portions of mulberry leaves can be selected from 0.5 portions, 0.6 portions, 0.7 portions, 0.8 portions, 0.9 portions, 1 portion, etc.; other specific point values ​​within the above numerical ranges can be selected, and for the sake of brevity, they will not be described one by one here.

[0021] Preferably, the Chinese herbal medicine fermentation product is prepared by the following preparation method:

[0022] The Chinese herbal medicine fermentation substrate is steamed and then cooled, and fermentation liquid is inoculated to perform anaerobic fermentation, and the product is dried and crushed to obtain the Chinese herbal medicine fermentation product.

[0023] Preferably, the steaming is carried out at 180-200°C (e.g., 180°C, 185°C, 190°C, 195°C, 200°C, etc.) for 30-40 min (e.g., 30 min, 32 min, 35 min, 38 min, 40 min, etc.).

[0024] Preferably, the total number of viable bacteria in the fermentation broth is not less than 5×10 5 CFU / mL, for example 5×10 5 CFU / mL, 1×10 6 CFU / mL, 5×10 6 CFU / mL, 1×10 7 CFU / mL, 5×107 CFU / mL, 1×10 8 CFU / mL, 5×10 8 CFU / mL, 1×10 9 CFU / mL, etc.

[0025] Preferably, the inoculation amount of the fermentation broth is 3-8%, such as 3%, 4%, 5%, 6%, 8%, etc.

[0026] Preferably, the fermentation is carried out at 32-38°C (eg, 32°C, 33°C, 34°C, 35°C, 36°C, 37°C, 38°C, etc.) for 7-10 days (eg, 7 days, 8 days, 9 days or 10 days).

[0027] Other specific point values ​​within the above numerical ranges can be selected, and for the sake of brevity, they will not be described here one by one.

[0028] Preferably, the Chinese herbal fermentation substrate is further pulverized before fermentation.

[0029] Preferably, the iodized salt is selected from any one of potassium iodate, potassium iodide or sodium iodide, or a combination of at least two of them.

[0030] In a second aspect, the present invention provides a laying duck feed for maintaining the intestinal health of ducks, wherein the laying duck feed comprises a basic laying duck feed and the feed additive described in the first aspect.

[0031] Preferably, the mass percentage of the feed additive in the feed is 1-10%, for example 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc. Other specific point values ​​within this numerical range can be selected. For the sake of brevity, they will not be described here one by one.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] The present invention creatively develops a new feed additive for ducks, which combines six components, namely, Chinese herbal medicine fermentation products, tea tree oil, stachyose, honeycomb, seaweed and iodized salt. Tea tree oil has a strong inhibitory effect on harmful intestinal bacteria and reduces the probability of intestinal bacterial infection; stachyose can promote the proliferation of beneficial intestinal bacteria and improve the activity of beneficial intestinal bacteria; honeycomb, seaweed and iodized salt work together to promote intestinal mucosal repair and maintain the health of intestinal mucosa. The formula is safe and non-toxic, and the components work together to improve the intestinal health of ducks, reduce intestinal diseases of ducks, improve duck immunity and improve the quality of duck eggs. DETAILED DESCRIPTION

[0034] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0035] The Lactobacillus plantarum, Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum mentioned below are commercially available strains ATCC8014, ATCC15703, ATCC15707 and ATCC29521, respectively.

[0036] Example 1

[0037] The present embodiment provides a feed additive for maintaining the intestinal health of ducks, which is obtained by mixing the following components: 5 parts of Chinese herbal fermentation product, 0.03 parts of tea tree oil, 2 parts of stachyose, 0.2 parts of honeycomb, 2 parts of alginate and 1.5 parts of potassium iodate; wherein the preparation method of the Chinese herbal fermentation product is as follows:

[0038] (1) 4 parts of alfalfa, 2 parts of Artemisia annua, 2 parts of Xanthium sibiricum, 2 parts of Polygonum hydropiper and 1 part of mulberry leaf are crushed and mixed, steamed at 180°C for 40 minutes, cooled and used as a fermentation substrate.

[0039] (2) Inoculate Lactobacillus plantarum, Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum strains into MRS liquid culture medium respectively, culture at 37° C. for 20 h for activation, and activate twice continuously to obtain activation solution; inoculate the activation solution into MRS liquid culture medium at an inoculum amount of 5% (v / v), ferment at 37° C. to obtain Lactobacillus plantarum fermentation solution, Bifidobacterium adolescentis fermentation solution, Bifidobacterium longum fermentation solution and Bifidobacterium bifidum fermentation solution; and dilute and mix as needed.

[0040] (3) Preparation of composite bacterial solution: The total number of viable bacteria in the composite bacterial solution is 5×10 5 CFU / mL, and the ratio of the viable counts of Lactobacillus plantarum, Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum was 10:2:2:1.

[0041] (4) The composite bacterial liquid is inoculated into the fermentation substrate at an inoculum amount of 5%, stirred and mixed, and then anaerobic fermentation is carried out at 38° C. for 10 days. The fermentation product is dried and crushed to obtain Chinese herbal medicine fermentation powder.

[0042] Example 2

[0043] The present embodiment provides a feed additive for maintaining the intestinal health of ducks, which is obtained by mixing the following components: 4 parts of Chinese herbal fermentation product, 0.05 parts of tea tree oil, 3 parts of stachyose, 0.5 parts of honeycomb, 3 parts of alginate and 1.5 parts of sodium iodide; wherein the preparation method of the Chinese herbal fermentation product is as follows:

[0044] (1) 5 parts of alfalfa, 1 part of Artemisia annua, 3 parts of Xanthium sibiricum, 1.5 parts of Polygonum hydropiper and 0.5 parts of mulberry leaf are crushed and mixed, steamed at 190° C. for 40 minutes, cooled and used as a fermentation substrate.

[0045] (2) Inoculate Lactobacillus plantarum, Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum strains into MRS liquid culture medium respectively, culture at 37° C. for 20 h for activation, and activate twice continuously to obtain activation solution; inoculate the activation solution into MRS liquid culture medium at an inoculum amount of 5% (v / v), ferment at 37° C. to obtain Lactobacillus plantarum fermentation solution, Bifidobacterium adolescentis fermentation solution, Bifidobacterium longum fermentation solution and Bifidobacterium bifidum fermentation solution; and dilute and mix as needed.

[0046] (3) Preparation of composite bacterial solution: The total number of viable bacteria in the composite bacterial solution is 1×10 6 CFU / mL, and the ratio of the viable counts of Lactobacillus plantarum, Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum was 5:3:1:1.

[0047] (4) The composite bacterial liquid is inoculated into the fermentation substrate at an inoculum rate of 7%, stirred and mixed, and then anaerobic fermentation is carried out at 35° C. for 12 days. The fermentation product is dried and crushed to obtain Chinese herbal medicine fermentation powder.

[0048] Example 3

[0049] The present embodiment provides a feed additive for maintaining the intestinal health of ducks, which is obtained by mixing the following components: 6 parts of Chinese herbal fermentation product, 0.01 parts of tea tree oil, 1 part of stachyose, 0.1 parts of honeycomb, 1 part of alginate and 1 part of potassium iodide; wherein the preparation method of the Chinese herbal fermentation product is as follows:

[0050] (1) 3 parts of alfalfa, 3 parts of Artemisia annua, 1 part of Xanthium sibiricum, 3 parts of Polygonum hydropiper and 1 part of mulberry leaf are crushed and mixed, steamed at 200°C for 30 minutes, cooled and used as a fermentation substrate.

[0051] (2) Inoculate Lactobacillus plantarum, Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum strains into MRS liquid culture medium respectively, culture at 37° C. for 20 h for activation, and activate twice continuously to obtain activation solution; inoculate the activation solution into MRS liquid culture medium at an inoculum amount of 5% (v / v), ferment at 37° C. to obtain Lactobacillus plantarum fermentation solution, Bifidobacterium adolescentis fermentation solution, Bifidobacterium longum fermentation solution and Bifidobacterium bifidum fermentation solution; and dilute and mix as needed.

[0052] (3) Preparation of composite bacterial solution: The total number of viable bacteria in the composite bacterial solution is 1×10 6 CFU / mL, and the ratio of the viable counts of Lactobacillus plantarum, Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum was 10:1:3:1.

[0053] (4) The composite bacterial liquid is inoculated into the fermentation substrate at an inoculum rate of 7%, stirred and mixed, and then anaerobic fermentation is carried out at 35° C. for 12 days. The fermentation product is dried and crushed to obtain Chinese herbal medicine fermentation powder.

[0054] Example 4

[0055] This embodiment provides a feed additive for maintaining the intestinal health of ducks. The only difference between its components and those of Example 1 is the preparation method of the Chinese herbal medicine fermentation product: alfalfa is missing in step (1), and its mass is proportionally distributed to the mass of the other four Chinese herbal medicines, and other operations remain unchanged.

[0056] Example 5

[0057] This embodiment provides a feed additive for maintaining the intestinal health of ducks. The only difference between its components and those of Example 1 is the different preparation method of the Chinese herbal medicine fermentation product: Artemisia annua is missing in step (1), and its mass is proportionally distributed to the mass of the other four Chinese herbal medicines, and other operations remain unchanged.

[0058] Example 6

[0059] This embodiment provides a feed additive for maintaining the intestinal health of ducks. The only difference between its components and those of Example 1 is the preparation method of the Chinese herbal fermentation product: in step (1), Xanthium sibiricum is missing, and its mass is proportionally distributed to the mass of the other four Chinese herbal medicines, and other operations remain unchanged.

[0060] Example 7

[0061] This embodiment provides a feed additive for maintaining the intestinal health of ducks. The only difference between its components and those of Example 1 is the different preparation method of the Chinese herbal fermentation product: in step (1), Polygonum hydropiper is missing, and its mass is proportionally distributed to the mass of the other four Chinese herbal medicines, and other operations remain unchanged.

[0062] Example 8

[0063] This embodiment provides a feed additive for maintaining the intestinal health of ducks. The only difference between its components and those of Example 1 is the different preparation method of the Chinese herbal fermentation product: mulberry leaves are missing in step (1), and their mass is proportionally distributed to the mass of the other four Chinese herbal medicines, and other operations remain unchanged.

[0064] Example 9

[0065] This embodiment provides a feed additive for maintaining the intestinal health of ducks. The difference between the components of this embodiment and those of the embodiment 1 is only the preparation method of the Chinese herbal medicine fermentation product: in step (1), 4 parts of alfalfa, 2 parts of Artemisia annua, 2 parts of Xanthium sibiricum, 2 parts of Polygonum hydropiper and 1 part of mulberry leaf are crushed and mixed without steaming, and directly used as a fermentation substrate for standby use. Other operations remain unchanged.

[0066] Example 10

[0067] This embodiment provides a feed additive for maintaining the intestinal health of ducks. The difference between its components and those of embodiment 1 is only the preparation method of the Chinese herbal medicine fermentation product: the fermentation liquid used is a single Lactobacillus plantarum liquid, and the total number of viable bacteria in the liquid is 5×10 5 CFU / mL, inoculated into the fermentation substrate at a 5% inoculum size, and other operations remained unchanged.

[0068] Embodiment 11

[0069] This embodiment provides a feed additive for maintaining the intestinal health of ducks. The difference between its components and those of embodiment 1 is only the preparation method of the Chinese herbal medicine fermentation product: the fermentation liquid used is a mixed liquid of Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum, and the total number of viable bacteria in the liquid is 5×10 5 CFU / mL, the ratio of the viable counts of Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum was 2:2:1, and they were inoculated in the fermentation substrate at a 5% inoculation rate, and other operations remained unchanged.

[0070] Example 12

[0071] This embodiment provides a feed additive for maintaining the intestinal health of ducks. The components of the feed additive are different from those of the embodiment 1 only in the preparation method of the Chinese herbal medicine fermentation product: the fermentation liquid used lacks Bifidobacterium adolescentis, and the reduced number of live bacteria is proportionally distributed to Bifidobacterium longum and Bifidobacterium bifidum, and the total number of live bacteria in the bacterial liquid is 5×10 5 CFU / mL, the ratio of the viable counts of Lactobacillus plantarum to Bifidobacterium was 2:1, and they were inoculated in the fermentation substrate at a 5% inoculum amount, and other operations remained unchanged.

[0072] Embodiment 13

[0073] This embodiment provides a feed additive for maintaining the intestinal health of ducks. The difference between its components and those of embodiment 1 is only the preparation method of the Chinese herbal medicine fermentation product: the fermentation liquid used lacks Bifidobacterium longum, and the reduced number of live bacteria is proportionally distributed to Bifidobacterium adolescentis and Bifidobacterium bifidum. The total number of live bacteria in the liquid is 5×10 5 CFU / mL, the ratio of the viable counts of Lactobacillus plantarum to Bifidobacterium was 2:1, and they were inoculated in the fermentation substrate at a 5% inoculum amount, and other operations remained unchanged.

[0074] Embodiment 14

[0075] This embodiment provides a feed additive for maintaining the intestinal health of ducks. The difference between its components and those of embodiment 1 is only the preparation method of the Chinese herbal medicine fermentation product: the fermentation liquid used lacks Bifidobacterium bifidum, and the reduced number of live bacteria is proportionally distributed to Bifidobacterium adolescentis and Bifidobacterium longum. The total number of live bacteria in the liquid is 5×10 5 CFU / mL, the ratio of the viable counts of Lactobacillus plantarum to Bifidobacterium was 2:1, and they were inoculated in the fermentation substrate at a 5% inoculum amount, and other operations remained unchanged.

[0076] Comparative Example 1

[0077] This comparative example provides a duck feed additive, the components of which differ from those of Example 1 only in the lack of tea tree oil, the reduced portion of which is distributed to the portion of stachyose, and the other components remain unchanged.

[0078] Comparative Example 2

[0079] This comparative example provides a duck feed additive, the components of which differ from those of Example 1 only in the lack of stachyose, the reduced amount of which is distributed to the amount of tea tree oil, and the other components remain unchanged.

[0080] Comparative Example 3

[0081] This comparative example provides a duck feed additive, the components of which differ from those of Example 1 only in the lack of honeycomb, and the reduced amount of honeycomb is proportionally distributed to the amounts of alginate and potassium iodate, while the other components remain unchanged.

[0082] Comparative Example 4

[0083] This comparative example provides a duck feed additive, the components of which differ from those of Example 1 only in the lack of alginate, and the reduced amount of alginate is proportionally distributed to the amounts of honeycomb and potassium iodate, while the other components remain unchanged.

[0084] Comparative Example 5

[0085] This comparative example provides a duck feed additive, the components of which differ from those of Example 1 only in the lack of potassium iodate, and the reduced amount thereof is proportionally distributed to the amounts of honeycomb and alginate, while the other components remain unchanged.

[0086] Test Example 1

[0087] 200 laying ducks aged 30 weeks were selected and divided into 20 groups, each group having 10 ducks, and fed according to the conventional breeding process of the farm for 6 weeks. The basic feed ingredients were: 60% corn, 25% soybean meal, 10% bran, and 5% stone powder. Groups 1-19 were fed with 4% additives prepared in Examples 1-14 and Comparative Examples 1-5 in the basic feed, and the remaining group was a control group, which was fed with the basic feed.

[0088] (1) After the feeding was completed, the feed-egg ratio of each group was counted, and 10 eggs were randomly selected from each group to measure the eggshell strength using an eggshell strength analyzer. The results are shown in Table 1.

[0089] Table 1

[0090]

[0091]

[0092] It can be seen from the data results in Table 1 that, compared with the comparative example, the duck feed additive involved in the present invention can significantly reduce the feed-egg ratio after intervening in laying ducks, improve production efficiency, and at the same time improve the eggshell strength of duck eggs and improve the quality of duck eggs, among which the six components of Chinese herbal fermentation products, tea tree oil, stachyose, honeycomb, alginate and iodized salt are indispensable. The fermentation substrate and fermentation strain used in the Chinese herbal fermentation products also affect the above indicators to a certain extent.

[0093] (2) After the feeding period, the ducks were fasted for 5 h. Serum was collected from the toe vein of each group and centrifuged at 3000 r / min and 4°C for 10 min to prepare serum. The level of immunoglobulin A (IgA, mg / mL) and total superoxide dismutase activity (U / mL) were determined by ELISA. The results are shown in Table 2.

[0094] Table 2

[0095]

[0096]

[0097] It can be seen from the data results in Table 2 that compared with the comparative example, the duck feed additive involved in the present invention can significantly improve the immunoglobulin level and antioxidant factor level in duck serum after intervening in laying ducks, that is, it can promote intestinal health and improve duck immunity and disease resistance, among which the six components of Chinese herbal fermentation products, tea tree oil, stachyose, honeycomb, alginate and iodized salt are indispensable. The fermentation substrate and fermentation strain used in the Chinese herbal fermentation products also affect the above indicators to a certain extent.

[0098] (3) Three laying ducks with a near-average body weight were randomly selected from each group for slaughter. The duodenum and jejunum were separated, and the middle 1.5 cm of the intestinal segment was fixed in 4% paraformaldehyde solution. The fixed intestinal segment was dehydrated with ethanol, transparentized with xylene, embedded in paraffin, and sliced. It was then stained with hematoxylin-eosin (HE) and observed under an optical microscope. The villus height (VH) and crypt depth (CD) of the intestinal segment were measured, and the ratio of villus height to crypt depth (villus-crypt ratio, VH / CD) was calculated, as shown in Table 3.

[0099] Table 3

[0100]

[0101]

[0102] From the data results in Table 3, it can be seen that the duck feed additive involved in the present invention can significantly increase the height of the villi in the duodenum and jejunum of ducks after intervening in laying ducks, while reducing the depth of the crypts in the duodenum and jejunum of ducks, and regulating the villus-crypt ratio of the duck intestine, that is, it can significantly improve the intestinal morphology and promote intestinal health, among which the six components of Chinese herbal fermentation products, tea tree oil, stachyose, honeycomb, alginate and iodized salt are all indispensable. And the fermentation substrate and fermentation strain used in the Chinese herbal fermentation products also affect the above indicators to a certain extent.

[0103] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0104] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0105] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A feed additive for maintaining the intestinal health of ducks, characterized in that: The feed additive comprises the following components: Chinese herbal medicine fermentation product, tea tree oil, stachyose, honeycomb, alginate and iodized salt.

2. The feed additive for maintaining duck intestinal health according to claim 1, characterized in that: The fermentation substrate of the Chinese herbal medicine fermentation product includes alfalfa, Artemisia annua, Xanthium sibiricum, Polygonum hydropiper and mulberry leaf; Preferably, the fermentation bacteria of the Chinese herbal medicine fermentation product include Lactobacillus plantarum and Bifidobacterium.

3. The feed additive for maintaining duck intestinal health according to claim 2, characterized in that: The ratio of the viable counts of Lactobacillus plantarum to Bifidobacterium is (1-3):1; Preferably, the bifidobacterium is selected from any one or a combination of at least two of Bifidobacterium adolescentis, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve or Bifidobacterium bifidum; more preferably, a combination of Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum; Preferably, the ratio of the viable counts of Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium bifidum is (1-3):(1-3):

1.

4. The feed additive for maintaining duck intestinal health according to any one of claims 1 to 3, characterized in that: The feed additive comprises, by weight: 3-7 parts of Chinese herbal medicine fermentation products, 0.01-0.05 parts of tea tree oil, 1-3 parts of stachyose, 0.1-0.5 parts of honeycomb, 1-3 parts of seaweed gel and 1-1.5 parts of iodized salt.

5. The feed additive for maintaining duck intestinal health according to claim 2, characterized in that: The fermentation substrate of the Chinese herbal medicine fermentation product comprises, by weight, 3-5 parts of alfalfa, 1-3 parts of Artemisia annua, 1-3 parts of Xanthium sibiricum, 1-3 parts of Polygonum hydropiper and 0.5-1 part of mulberry leaf.

6. The feed additive for maintaining duck intestinal health according to any one of claims 1 to 5, characterized in that: The Chinese herbal medicine fermentation product is prepared by the following preparation method: The Chinese herbal medicine fermentation substrate is steamed and then cooled, and fermentation liquid is inoculated to perform anaerobic fermentation, and the product is dried and crushed to obtain the Chinese herbal medicine fermentation product.

7. The feed additive for maintaining duck intestinal health according to claim 6, characterized in that: The steaming is performed at 180-200° C. for 30-40 min; Preferably, the total number of viable bacteria in the fermentation broth is not less than 5×10 5 CFU / mL; Preferably, the inoculation amount of the fermentation broth is 3-8%; Preferably, the fermentation is carried out at 32-38°C for 7-10 days; Preferably, the Chinese herbal fermentation substrate is further pulverized before fermentation.

8. The feed additive for maintaining duck intestinal health according to any one of claims 1 to 7, characterized in that: The iodized salt is selected from any one of potassium iodate, potassium iodide or sodium iodide, or a combination of at least two of them.

9. A laying duck feed for maintaining duck intestinal health, characterized in that: The laying duck feed comprises a basic laying duck feed and the feed additive according to any one of claims 1 to 8.

10. The laying duck feed according to claim 9, characterized in that: The mass percentage of the feed additive in the feed is 1-10%.

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