GABA (gamma-aminobutyric acid)-containing anti-alcohol composition as well as preparation method and application thereof

Abstract

The invention discloses a GABA (gamma-aminobutyric acid)-containing anti-alcohol composition as well as a preparation method and application thereof, and belongs to the technical field of foods. The invention provides a GABA (gamma-aminobutyric acid)-containing anti-alcoholism composition. The composition is prepared from the following components: gamma-aminobutyric acid, a radix puerariae extract, a semen hoveniae extract, vitamin B1, vitamin B6, a fructus mume extract, a lalang grass rhizome extract, corn oligopeptide, taurine, baicalin and isorhamnetin. The composition disclosed by the invention can effectively relieve the harm of alcohol to a human body.

Description

Technical Field

[0001] The present invention belongs to the field of food technology, and in particular relates to a hangover-relieving composition containing GABA and a preparation method and application thereof. Background Art

[0002] Wine is a carrier of Chinese culture, which contains rich historical significance. It is also a common drink at gatherings, restaurants, and banquets. However, excessive drinking can lead to alcohol poisoning, which is very harmful to the body. The mechanism of alcohol causing human drunkenness includes the following three aspects: First, the human body ingests a large amount of alcohol in a short period of time, which makes the body unable to metabolize quickly, resulting in the accumulation of ethanol. The increase in the ethanol content in the blood will lead to its obvious inhibition of the central nervous system, and then the state of drunkenness such as drowsiness or coma will occur. Second, the alcohol that enters the human body will be decomposed into acetaldehyde, which is then decomposed into acetic acid by ALDH, but the ALDH enzyme activity of individuals varies greatly. For people with low ALDH enzyme activity, the acetaldehyde in the body cannot be quickly decomposed and metabolized into acetic acid, but continues to remain in the body, causing people to have nausea, coma, and other drunken states; in addition, acetaldehyde is a muscle toxin that can damage the liver. Third, when the human body ingests a large amount of ethanol, the high ethanol concentration will induce the enhanced activity of the recombinant protein of cytochrome P450 family member 2E1 (recombinant cytochrome P450 2E1, CYP2E1). At this time, ethanol will be mainly metabolized by CYP2E1 and produce a large number of oxygen free radicals, which can consume many reducing protective substances, cause lipid peroxidation reactions in tissues, and ultimately lead to liver damage.

[0003] γ-Aminobutyric acid (GABA) is a naturally occurring non-protein amino acid that acts as an important inhibitory neurotransmitter in the human central nervous system. It reduces the effects of alcohol on the body mainly through the following mechanisms during the hangover process: First, it reduces the burden on the liver. Alcohol is mainly metabolized in the liver. Most of the alcohol is first converted into acetaldehyde by alcohol dehydrogenase (ADH), and then converted into acetic acid by acetaldehyde dehydrogenase (ALDH), and finally decomposed into carbon dioxide and water and excreted from the body. GABA can enhance the antioxidant capacity of liver cells by regulating the metabolic state of liver cells; second, it promotes the activity of metabolic enzymes. GABA may have a certain regulatory effect on the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver. Although the specific molecular mechanism is not fully understood, some studies have shown that GABA can increase the activity of these metabolic enzymes to a certain extent and accelerate the metabolic decomposition process of alcohol in the liver; third, it relieves gastrointestinal spasm. Alcohol has a stimulating effect on the gastrointestinal tract and is prone to spasm of gastrointestinal smooth muscle. GABA can act on receptors on gastrointestinal smooth muscle to regulate the contraction of smooth muscle. It can relax spasmodic smooth muscles and relieve gastrointestinal discomfort, such as abdominal pain and nausea. Fourth, it protects the gastrointestinal mucosa. GABA can promote the repair and regeneration of gastrointestinal mucosal cells. Alcohol can damage the gastrointestinal mucosa and destroy the barrier function of the mucosa. GABA helps repair damaged mucosa by regulating the growth factors and intercellular signaling pathways of mucosal cells, enhances the defense ability of the gastrointestinal mucosa, and reduces further damage to the gastrointestinal tract caused by alcohol.

[0004] Therefore, it is very necessary to develop a hangover-relieving composition containing GABA. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a GABA hangover-relieving composition which can effectively alleviate the damage caused by alcohol to the human body in view of the prior art.

[0006] To achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a GABA hangover-relieving composition, the composition is composed of the following components in parts by mass: 10-30 parts of γ-aminobutyric acid, 30-50 parts of kudzu root extract, 20-30 parts of Hovenia dulcis extract, 0.5-1 parts of vitamin B1, 0.5-1 parts of vitamin B6, 2-5 parts of black plum extract, 2-5 parts of Imperatae cylindrica root extract, 2-5 parts of corn oligopeptides, 2-6 parts of taurine, 1-2 parts of baicalin, and 1-2 parts of isorhamnetin.

[0007] Furthermore, the preparation method of the capsule comprises the following steps: (1) Tween 80 and Span 80 are compounded as emulsifiers, and the total amount of emulsifiers is 2-3% of the total weight of the hangover-relieving composition. The emulsifiers are slowly added into deionized water at 40-50°C, stirring at a speed of 500-800 rpm. A uniform emulsifier solution is prepared and set aside.

[0008] (2) adding γ-aminobutyric acid, kudzu root extract, Hovenia dulcis extract, vitamin B1, vitamin B6, ebony extract, Imperata cylindrica root extract, corn oligopeptide, taurine, baicalin and isorhamnetin to the emulsifier solution of step (1) for emulsification to obtain an emulsified solution; (3) Weigh appropriate amounts of gelatin and arabic respectively, stir and dissolve them in hot water at 50-60℃ until the solution becomes clear and transparent to obtain a wall material solution.

[0009] (4) The emulsified solution of step (2) is slowly added dropwise to the wall material solution of step (3), and mixed at 50-60°C and a stirring speed of 400-600 rpm for 15-35 min. After uniform dispersion, 1-1.5 mol / L acetic acid solution is slowly added dropwise to adjust the pH value of the system to 4.2-4.5 for 20-30 min. A coagulation reaction occurs, and gelatin and gum arabic undergo a complex coagulation reaction to form a microcapsule wall on the surface of the raw material droplets. The acetic acid addition process needs to be slow and stirred continuously to prevent uneven coagulation due to excessively low local pH value.

[0010] (5) After the condensation reaction in step (4) is completed, the microcapsules are prepared by aging at 5-10°C for 40-60 minutes to further solidify the microcapsule wall and enhance the stability of the microcapsules.

[0011] (6) The microcapsules prepared in step (5) are centrifuged, washed, and vacuum freeze-dried.

[0012] Furthermore, the mass ratio of Tween 80 to Span 80 in step (1) is 3:1.

[0013] Furthermore, the emulsification temperature of step (2) is 50-60°C, the shear rate is 10000-12000rpm, and the emulsification time is 8-12 min.

[0014] Furthermore, in the wall material solution of step (3), the mass fraction of the gelatin solution is 5% - 8%, and the mass fraction of the gum arabic solution is 3% - 6%.

[0015] Furthermore, the mass ratio of gelatin to gum arabic in step (3) is 1.5:1.

[0016] Furthermore, the stirring speed of step (3) is 300 - 500 rpm.

[0017] Furthermore, the volume ratio of the emulsified solution to the wall material solution in step (4) is (0.8-1):1.

[0018] Furthermore, the centrifugal speed of step (6) is 4000-6000 rpm, the time is 15-20 min, and the washing is repeated 3-4 times, and the wall material, emulsifier, and unembedded raw material impurities remaining on the surface are removed. The microcapsule is pre-frozen at -45-50°C for 3-4 hours, and then sublimated and dried at a vacuum degree of 20-40 Pa for 12-16 hours to retain the active ingredients and structural integrity of the raw materials in the microcapsule to the greatest extent.

[0019] The present invention also provides the use of the above-mentioned GABA hangover-relief composition in hangover-relief products.

[0020] In the above technical scheme, GABA is one of the main ingredients. GABA can regulate the nervous system, relieve anxiety and tension after drinking, and promote the metabolism of ethanol; Pueraria root extract accelerates alcohol metabolism, reduces alcohol damage to the liver, and relieves headaches and dizziness; Hovenia dulcis extract increases the activity of liver alcohol dehydrogenase and promotes alcohol decomposition and metabolism; Vitamin B1 maintains the normal function of the nervous system and promotes liver metabolism; Vitamin B6 participates in alcohol metabolism and reduces the adverse effects of alcohol on the nervous system; Plum extract promotes thirst and relieves discomfort symptoms after drinking Imperata root extract, clears away heat and dampness, relieves symptoms of discomfort after drinking; corn oligopeptides, activates alcohol dehydrogenase activity, inhibits alcohol poisoning; taurine, strengthens the liver and promotes bile secretion, relieves fever and inflammation; baicalin, is an effective antioxidant, its molecular structure contains phenolic hydroxyl groups, these phenolic hydroxyl groups can provide hydrogen atoms, react with free radicals, thereby removing free radicals, and can also regulate the body's antioxidant enzyme system, thereby reducing the oxidative damage caused by alcohol; isorhamnetin, can reduce the impact of alcohol on the nervous system by regulating the level of neurotransmitters, it can also increase the content of GABA or enhance its function, play a sedative and anti-anxiety role, and relieve the symptoms of nervous system excitement caused by alcohol.

[0021] Compared with the prior art, the present invention has the following beneficial effects: (1) The GABA hangover-relief composition provided by the present invention is compounded with 11 ingredients, which can accelerate the decomposition of acetaldehyde in the liver, promote metabolism, protect the liver, and achieve excellent hangover-relief effects.

[0022] (2) The present invention prepares microcapsules by embedding the hangover-relieving composition containing GABA. The hangover-relieving effect of the microcapsules of the GABA hangover-relieving composition is more significant. Since GABA and other substances are easily affected by the acid-base environment, the unencapsulated GABA may undergo chemical reactions in the acidic environment of the gastrointestinal tract (pH 1.5-3.5) or the alkaline digestive fluid (pH 7.5-9.0), such as decarboxylation reaction, which leads to loss of activity. The microcapsules can buffer the direct effect of the acid-base environment on the core material to a certain extent. When the microcapsules enter the gastrointestinal tract, the wall material slowly dissolves or swells, and the core material is gradually released, so that GABA and other components are in contact with the digestive fluid in a relatively mild local environment, thereby reducing the probability of chemical reactions. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0024] The reagents used in the present invention are all commercially available food grade, and unless otherwise specified, are all conventional reagents, methods and equipment in the art.

[0025] Example 1 The embodiment of the present invention provides a hangover-relieving composition containing GABA, which is composed of the following components in parts by mass: 20 parts of γ-aminobutyric acid, 40 parts of kudzu root extract, 25 parts of Hovenia dulcis extract, 0.7 parts of vitamin B1, 0.7 parts of vitamin B6, 3.5 parts of black plum extract, 3.5 parts of Imperata root extract, 4 parts of corn oligopeptides, 4 parts of taurine, 1.5 parts of baicalin, and 1.5 parts of isorhamnetin.

[0026] The embodiment of the present invention provides a method for preparing a capsule containing a GABA hangover-relieving composition, comprising the following steps: (1) Tween 80 and Span 80 are mixed in a mass ratio of 3:1 as emulsifiers. The total amount of the emulsifier is 2.5% of the total mass of the hangover-relieving composition. The emulsifier is slowly added into deionized water at 45°C, stirring at a stirring speed of 650 rpm. A uniform emulsifier solution is prepared and set aside.

[0027] (2) Add the above-mentioned GABA hangover-relieving composition according to the formula amount into the emulsifier solution of step (1) for emulsification at a temperature of 55° C., a shear rate of 11,000 rpm, and a time of 10 min to obtain an emulsified solution.

[0028] (3) Weigh gelatin and gum arabic in a mass ratio of 1.5:1, where the mass fraction of gelatin solution is 6% and the mass fraction of gum arabic solution is 4%, and dissolve them in hot water at 55°C at a stirring speed of 400 rpm until the solution is clear and transparent to obtain a wall material solution.

[0029] (4) The emulsified solution of step (2) was slowly added dropwise to the wall material solution of step (3), wherein the volume ratio of the emulsified solution to the wall material solution was 0.9:1, and the mixture was mixed at 55°C and a stirring speed of 500 rpm for 25 min. After uniform dispersion, 1.2 mol / L acetic acid solution was slowly added dropwise for 25 min to adjust the pH value of the system to 4.3.

[0030] (5) After the condensation reaction in step (4) is completed, the mixture is aged at 8°C for 50 min to obtain microcapsules.

[0031] (6) The microcapsules prepared in step (5) were centrifuged at 5000 rpm for 18 min, washed four times with deionized water, pre-frozen at -48°C for 3.5 h, and then sublimated and dried at a vacuum of 3 Pa for 14 h.

[0032] Example 2 The embodiment of the present invention provides a hangover-relieving composition containing GABA, which is composed of the following components in parts by mass: 10 parts of γ-aminobutyric acid, 30 parts of kudzu root extract, 20 parts of Hovenia dulcis extract, 0.5 parts of vitamin B1, 0.5 parts of vitamin B6, 2 parts of black plum extract, 2 parts of Imperata root extract, 2 parts of corn oligopeptides, 2 parts of taurine, 1 part of baicalin, and 1 part of isorhamnetin.

[0033] The embodiment of the present invention provides a method for preparing a capsule containing a GABA hangover-relieving composition, comprising the following steps: (1) Tween 80 and Span 80 were mixed in a mass ratio of 3:1 as emulsifiers. The total amount of the emulsifier was 2% of the total mass of the hangover-relieving composition. The emulsifier was slowly added into deionized water at 40°C, stirring at a speed of 500 rpm. A uniform emulsifier solution was prepared and set aside.

[0034] (2) Add the above-mentioned GABA hangover-relieving composition according to the formula amount into the emulsifier solution of step (1) for emulsification at a temperature of 50°C, a shear rate of 10,000 rpm, and a time of 8 minutes to obtain an emulsified solution.

[0035] (3) Weigh gelatin and gum arabic in a mass ratio of 1.5:1, where the mass fraction of gelatin solution is 5% and the mass fraction of gum arabic solution is 3%, and dissolve them in hot water at 50°C at a stirring speed of 300 rpm until the solution is clear and transparent to obtain a wall material solution.

[0036] (4) The emulsified solution of step (2) was slowly added dropwise to the wall material solution of step (3), wherein the volume ratio of the emulsified solution to the wall material solution was 0.8):1, and the mixture was mixed at 50°C and a stirring speed of 400 rpm for 15 min. After uniform dispersion, 1 mol / L acetic acid solution was slowly added dropwise for 20 min to adjust the pH value of the system to 4.2.

[0037] (5) After the condensation reaction in step (4) is completed, the mixture is aged at 5°C for 40 minutes to obtain microcapsules.

[0038] (6) The microcapsules prepared in step (5) were centrifuged at 4000 rpm for 20 min, washed four times with deionized water, pre-frozen at -45 °C for 3 h, and then sublimated and dried at a vacuum of 20 Pa for 16 h.

[0039] Example 3 The embodiment of the present invention provides a hangover-relieving composition containing GABA, which is composed of the following components in parts by mass: 30 parts of γ-aminobutyric acid, 50 parts of kudzu root extract, 30 parts of Hovenia dulcis extract, 1 part of vitamin B1, 1 part of vitamin B6, 5 parts of black plum extract, 5 parts of Imperata root extract, 5 parts of corn oligopeptides, 6 parts of taurine, 2 parts of baicalin, and 2 parts of isorhamnetin.

[0040] The embodiment of the present invention provides a method for preparing a capsule containing a GABA hangover-relieving composition, comprising the following steps: (1) Tween 80 and Span 80 are mixed as emulsifiers in a mass ratio of 3:1. The total amount of the emulsifier is 3% of the total mass of the hangover-relieving composition. The emulsifier is slowly added into deionized water at 50°C, stirring at a speed of 800 rpm. A uniform emulsifier solution is prepared and set aside.

[0041] (2) Add the above-mentioned GABA hangover-relieving composition according to the formula amount into the emulsifier solution of step (1) for emulsification at a temperature of 60° C., a shear rate of 12,000 rpm, and a time of 12 min to obtain an emulsified solution.

[0042] (3) Weigh gelatin and gum arabic in a mass ratio of 1.5:1, where the mass fraction of gelatin solution is 8% and the mass fraction of gum arabic solution is 6%, and dissolve them in hot water at 60°C with a stirring speed of 500 rpm until the solution is clear and transparent to obtain a wall material solution.

[0043] (4) The emulsified solution of step (2) was slowly added dropwise to the wall material solution of step (3), wherein the volume ratio of the emulsified solution to the wall material solution was 1:1, and the mixture was mixed at 60°C and a stirring speed of 600 rpm for 35 min. After uniform dispersion, 1.5 mol / L acetic acid solution was slowly added dropwise for 30 min to adjust the pH value of the system to 4.5.

[0044] (5) After the condensation reaction in step (4) is completed, the mixture is aged at 10°C for 60 min to obtain microcapsules.

[0045] (6) The microcapsules prepared in step (5) were centrifuged at 6000 rpm for 15 min, washed four times with deionized water, pre-frozen at -50 °C for 3 h, and then sublimated and dried at a vacuum of 40 Pa for 12 h.

[0046] Comparative Example 1 An embodiment of the present invention provides a hangover-relieving composition, which is composed of the following components in parts by mass: 40 parts of kudzu root extract, 25 parts of Hovenia dulcis extract, 0.7 parts of vitamin B1, 0.7 parts of vitamin B6, 3.5 parts of black plum extract, 3.5 parts of Imperata cylindrica root extract, 4 parts of corn oligopeptides, 4 parts of taurine, 1.5 parts of baicalin, and 1.5 parts of isorhamnetin.

[0047] The embodiment of the present invention provides a method for preparing a capsule of a hangover-relieving composition, comprising the following steps: (1) Tween 80 and Span 80 are mixed in a mass ratio of 3:1 as emulsifiers. The total amount of the emulsifier is 2.5% of the total mass of the hangover-relieving composition. The emulsifier is slowly added into deionized water at 45°C, stirring at a stirring speed of 650 rpm. A uniform emulsifier solution is prepared and set aside.

[0048] (2) Add the above-mentioned hangover-relieving composition according to the formula amount into the emulsifier solution of step (1) for emulsification at a temperature of 55° C., a shear rate of 11,000 rpm, and a time of 10 min to obtain an emulsified solution.

[0049] (3) Weigh gelatin and gum arabic in a mass ratio of 1.5:1, where the mass fraction of gelatin solution is 6% and the mass fraction of gum arabic solution is 4%, and dissolve them in hot water at 55°C at a stirring speed of 400 rpm until the solution is clear and transparent to obtain a wall material solution.

[0050] (4) The emulsified solution of step (2) was slowly added dropwise to the wall material solution of step (3), wherein the volume ratio of the emulsified solution to the wall material solution was 0.9:1, and the mixture was mixed at 55°C and a stirring speed of 500 rpm for 25 min. After uniform dispersion, 1.2 mol / L acetic acid solution was slowly added dropwise for 25 min to adjust the pH value of the system to 4.3.

[0051] (5) After the condensation reaction in step (4) is completed, the mixture is aged at 8°C for 50 min to obtain microcapsules.

[0052] (6) The microcapsules prepared in step (5) were centrifuged at 5000 rpm for 18 min, washed four times with deionized water, pre-frozen at -48°C for 3.5 h, and then sublimated and dried at a vacuum of 3 Pa for 14 h.

[0053] Comparative Example 2 The embodiment of the present invention provides a hangover-relieving composition containing GABA, which is composed of the following components in parts by mass: 20 parts of γ-aminobutyric acid, 40 parts of kudzu root extract, 25 parts of Hovenia dulcis extract, 0.7 parts of vitamin B1, 0.7 parts of vitamin B6, 3.5 parts of black plum extract, 3.5 parts of Imperata root extract, 4 parts of corn oligopeptides, 4 parts of taurine, 1.5 parts of baicalin, and 1.5 parts of isorhamnetin.

[0054] The embodiment of the present invention provides a method for preparing a GABA hangover-relieving composition tablet: the above raw materials are mixed evenly and then put into a rotary tablet press for tableting. The pressure range is 12 kN. The tableting speed is 28 tablets / min.

[0055] Experimental example Animal hangover experiment: 50 ICR mice were divided into five groups, namely a blank control group (administered with pure water), a model group (administered with liquor at a dose of 0.15 ml / 10 g), an experimental group (the experimental group was Example 1 and Comparative Examples 1-2, each group was administered with liquor at a dose of 0.15 ml / 10 g, and the dose of the composition of each group was 0.5 g / kg. The disappearance of the righting reflex (drunk) of the mouse was considered a successful drunkenness model, and the drunkenness time and sobering time of the mouse were observed and recorded. The results are shown in Table 1 below.

[0056] Table 1 The drunkenness time and soberness time of mice

[0057] As can be seen from Table 1, the drunkenness time of Example 1 in the experimental group is longer than that of the comparative examples 1-2, and the sobering time is shorter than that of the model group, indicating that the microcapsules containing the GABA alcohol sobering composition prepared by the present invention have obvious alcohol sobering effects. During this experiment, the blank control mice were always in a normal state, and the model group mice were drunk at a corresponding time after being fed with alcohol, indicating that the drunkenness model was successful.

[0058] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A hangover-relief composition comprising GABA, characterized in that: The composition consists of the following components in parts by mass: 10-30 parts of γ-aminobutyric acid, 30-50 parts of kudzu root extract, 20-30 parts of Hovenia dulcis extract, 0.5-1 parts of vitamin B1, 0.5-1 parts of vitamin B6, 2-5 parts of black plum extract, 2-5 parts of Imperata root extract, 2-5 parts of corn oligopeptides, 2-6 parts of taurine, 1-2 parts of baicalin, and 1-2 parts of isorhamnetin.

2. A GABA hangover-relief composition according to claim 1, characterized in that: The preparation method of the capsule containing the GABA hangover-relieving composition comprises the following steps: Tween 80 and Span 80 are compounded as emulsifiers, and the total amount of the emulsifier is 2-3% of the total weight of the hangover-relieving composition. The emulsifier is slowly added into deionized water at 40-50°C, and stirred at a speed of 500-800rpm for 10-20min to prepare a uniform emulsifier solution; Adding γ-aminobutyric acid, kudzu root extract, Hovenia dulcis extract, vitamin B1, vitamin B6, ebony extract, Imperata cylindrica root extract, corn oligopeptide, taurine, baicalin and isorhamnetin to the emulsifier solution of step (1), emulsifying and shearing to obtain an emulsified solution; Stir gelatin and Arabica in hot water at 50-60℃ to dissolve until the solution is clear and transparent to obtain a wall material solution; Slowly drop the emulsified solution of step (2) into the wall material solution of step (3), mix at 50-60°C and stirring speed of 400-600 rpm for 15-35 min, and after uniform dispersion, slowly drop 1-1.5 mol / L acetic acid solution, adjust the pH value to 4.2-4.5, and mix for 20-30 min to cause a coagulation reaction; After the condensation reaction in step (4) is completed, the mixture is aged at 5-10°C for 40-60 minutes to obtain microcapsules; The microcapsules in step (5) are washed by centrifugation and freeze-dried in vacuum.

3. A hangover-relieving composition containing GABA according to claim 2, characterized in that: The mass ratio of Tween 80 to Span 80 in the step (1) is 3:

1.

4. A hangover-relief composition containing GABA according to claim 2, characterized in that: The emulsification temperature of step (2) is 50-60°C, the shear rate is 10000-12000 rpm, and the emulsification time is 8-12 min.

5. A hangover-relief composition containing GABA according to claim 2, characterized in that: The mass fraction of the gelatin solution in the wall material solution of step (3) is 5% to 8%, and the mass fraction of the gum arabic solution is 3% to 6%.

6. A GABA hangover-relief composition according to claim 2, characterized in that: The mass ratio of gelatin to gum arabic in step (3) is 1.5:1; the stirring speed in step (3) is 300-500 rpm.

7. A hangover-relief composition containing GABA according to claim 2, characterized in that: The volume ratio of the emulsified solution to the wall material solution in step (4) is (0.8-1):

1.

8. The hangover-relief composition containing GABA according to claim 2, characterized in that: The centrifugal speed of step (6) is 4000-6000 rpm, the time is 15-20 min, the washing is repeated 3-4 times, and the sample is pre-frozen at -45-50°C for 3-4 h, and then dried at a vacuum degree of 20-40 Pa for 12-16 h.

9. Use of the GABA hangover-relief composition according to any one of claims 1 to 8 in hangover-relief products.

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