A magnetically compatible rodent brain injection device and method

Abstract

The present invention belongs to the technical field of magnetic resonance imaging, and specifically discloses a magnetically compatible rodent brain injection device and method, which includes a fixed base, an injection catheter system, and a drug delivery system. The bottom surface of the fixed base is fixedly connected to the surface of the skull through an adhesive. A stereotactic fine-tuning system is provided on the top surface of the fixed base. The stereotactic fine-tuning system is fixedly connected to the outer ring of the injection catheter system. One end of the injection catheter system sequentially passes through the stereotactic fine-tuning system and the fixed base and is connected to the interior of the skull. The other end of the injection catheter system is connected to the drug delivery system. By adopting the above-mentioned magnetically compatible rodent brain injection device and method, the present invention avoids signal artifacts or interference, has both imaging compatibility and operation convenience, realizes high-precision, good repeatability, and small damage to brain tissue for intracerebral drug injection, and provides a reliable technical means for brain research.

Description

Technical Field

[0001] The present invention relates to the technical field of magnetic resonance imaging, and in particular to a magnetically compatible rodent brain injection device and method. Background Art

[0002] Magnetic resonance imaging (MRI) is a non-invasive medical imaging technique that can provide high-resolution images of the internal structure of tissues, especially having unique advantages in soft tissue imaging. The application of MRI technology in the field of neuroimaging is becoming increasingly widespread, which can non-invasively observe the structural and functional changes of the brain tissue under physiological and pathological conditions and accurately locate specific brain regions. In the research of brain disease models, rodents are often used as experimental subjects for imaging studies, and drug injection into the brain parenchyma or ventricular region is often an indispensable part.

[0003] In the prior art, traditional drug delivery devices usually adopt stainless steel materials. These devices are suitable for chronic drug delivery outside MRI, but have limitations when used in the nuclear magnetic resonance scanning environment. Especially during acute modeling or acute drug delivery, the traditional stainless steel drug delivery cannula cannot be used normally under MRI scanning, and real-time imaging scanning before and after drug delivery cannot be achieved. In addition, the traditional drug delivery cannula has many limitations. Its injection depth needs to be customized and it is difficult to adjust flexibly; the positioning catheter needs to penetrate deep into the animal's brain, and its relatively large outer diameter is likely to cause craniocerebral injury; in addition, the catheter base is relatively thick, so that the surface coil cannot be closely attached to and wrap the skull during MRI imaging, thus affecting the imaging quality.

[0004] Therefore, developing a rodent brain injection cannula device that can be stably used in a magnetic resonance environment can enable the brain injection operation to proceed smoothly during MRI scanning, thereby promoting the smooth development of relevant imaging studies and experiments. Summary of the Invention

[0005] The purpose of the present invention is to provide a magnetically compatible rodent brain injection device and method, which can avoid signal artifacts or interference, have both imaging compatibility and operation convenience, realize high-precision, good repeatability, and small brain tissue damage brain drug injection, successfully realize liquid distribution monitoring and position calibration, and provide a reliable technical means for brain science, drug research and development, and neurological disease research.

[0006] To achieve the above object, the present invention provides a magnetically compatible rodent brain injection device, including a fixed base, an injection catheter system, and a drug delivery system. The bottom surface of the fixed base is fixedly connected to the surface of the skull through a cementing agent. The top surface of the fixed base is provided with a stereotaxic fine adjustment system, and the stereotaxic fine adjustment system is fixedly connected to the outer ring of the injection catheter system. One end of the injection catheter system sequentially penetrates through the stereotaxic fine adjustment system and the fixed base and communicates with the interior of the skull, and the other end of the injection catheter system communicates with the drug delivery system.

[0007] Preferably, the bottom surface of the fixed base is provided with a plurality of fixing notches, and the cementing agent is one or both of dental cement or bone cement.

[0008] Preferably, the stereotaxic fine adjustment system includes a fine adjustment component and a fixing component. The fine adjustment component includes an X-axis track and a Y-axis track. Both the X-axis track and the Y-axis track are provided with threaded knobs. The bottom surface of the X-axis track is slidably connected to the fixed base through the threaded knob, and the bottom surface of the Y-axis track is slidably connected to the top surface of the X-axis track through the threaded knob. The fixing component is arranged at the center position of the top surface of the Y-axis track.

[0009] Preferably, the injection catheter system includes a drug delivery cannula and a positioning catheter sleeved on the outer wall of the drug delivery cannula. One end of the positioning catheter is fixedly connected to the fixing component, and the other end of the positioning catheter is provided with a fastening knob. The drug delivery cannula is fixedly connected to the positioning catheter through the fastening knob, and the outer wall of the fastening knob is provided with anti-slip threads.

[0010] Preferably, the outer diameter of the drug delivery cannula is equal to the inner diameter of the positioning catheter, and the length of the drug delivery cannula is greater than the length of the positioning catheter.

[0011] Preferably, the drug delivery cannula is made of quartz material, and the positioning catheter and the fastening knob are both made of non-metallic materials.

[0012] Preferably, both the fixed base and the stereotaxic fine adjustment system are made of non-metallic materials, specifically one of polyetheretherketone or Teflon.

[0013] Preferably, the drug delivery system includes a PE tube, a microsyringe, and a microinjection pump, and the microsyringe adopts a threaded knob propulsion design.

[0014] On the other hand, the present invention also provides a magnetically compatible rodent brain injection method, using the above-mentioned magnetically compatible rodent brain injection device, including the following steps:

[0015] S1. Fixation and positioning: Fix the animal on the stereotaxic apparatus, ensure that the surface of the head is parallel to the horizontal plane of the stereotaxic apparatus, open the scalp of the animal to expose the skull, drill a hole in the skull above the target area to a depth reaching the surface of the dura mater;

[0016] S2. Installation of the fixation base and the positioning catheter: Bond the fixation base to the skull around the drilled hole, and after curing, install the positioning catheter and lock it on the stereotaxic fine adjustment system. By adjusting the XY directions, make the positioning catheter opposite to the drilled hole;

[0017] S3. Preparation of the drug delivery cannula: Connect the drug delivery cannula pre-filled with a set dose of injection liquid to a PE tube pre-filled with physiological saline. The PE tube penetrates both inside and outside the nuclear magnetic resonance environment. The external PE tube is connected to a micro syringe and fixed on a micro infusion pump;

[0018] S4. Animal MRI scanning: After transferring the animal obtained in S2 to the scanning workshop, fix it and monitor the physiological indicators, then insert the drug delivery cannula obtained in S3 into the positioning catheter. After reaching the target depth, use the knob to fix the drug delivery cannula and the positioning catheter;

[0019] S5. Check whether the positioning of the target area is accurate through magnetic resonance structural imaging. If the target area is not reached, repeat S4 until the positioning is accurate;

[0020] S6. Drug injection: Start the micro infusion pump, inject the set dose of injection liquid, physiological saline and blood at the preset rate, and use MRI for imaging to check the distribution of the liquid in the brain;

[0021] S7. Removal of the drug delivery cannula: If subsequent experimental injections need to be performed at the same position, install a protective cover to cover the positioning catheter at the drilled hole position. If no subsequent experiments are to be carried out, the positioning catheter can be removed and the wound can be sealed.

[0022] Preferably, S6 further includes the following steps:

[0023] Start the micro infusion pump, first inject the liquid into the deep nuclear mass at the preset rate and set dose, let it stand, then use MRI for imaging, then adjust the depth of the drug delivery cannula upward, and then fix it with the fastening knob. Inject the set dose of liquid into the cerebral cortex and check the distribution of the liquid in the brain.

[0024] Therefore, the present invention adopts the above-mentioned magnetic compatibility rodent brain injection device and method, and the beneficial effects are as follows:

[0025] (1) Strong magnetic compatibility: The device of the present invention uses non-magnetic materials and can operate stably in the strong magnetic field environment of MRI without being interfered by the magnetic field, ensuring the image quality during the MRI imaging process and the accuracy of the drug injection operation. The injection operation and MRI imaging can be carried out synchronously, improving the efficiency and accuracy of the experiment.

[0026] (2) Precise positioning: Through a high-precision three-dimensional positioning fine-tuning device and in combination with a positioning catheter, the present invention enables the drug delivery cannula to accurately align with the predetermined hole position on the skull of the experimental animal, ensuring that the drug is accurately injected into the target brain region, avoiding drug leakage or misinjection, thereby reducing errors during the drug injection process. The positioning catheter does not need to penetrate deep into the brain, thus reducing damage to brain tissue.

[0027] (3) High safety: The device of the present invention can minimize harm to experimental animals. The drug delivery cannula is made of glass material and is fixed on the positioning catheter through a tightening knob to ensure its stability during injection. After the injection, the drug delivery cannula can be safely removed without causing continuous harm to the animal, and the protective cover can effectively protect the wound, avoiding infection and injury.

[0028] (4) Real-time monitoring: Through the MRI device, the present invention can monitor the distribution and diffusion of drugs in the brain in real time, enabling researchers to accurately observe the effects of drugs.

[0029] (5) The device of the present invention has a compact structure design and is easy to operate. It can quickly complete drug injection during the experiment and can observe the reaction of brain tissue in real time under MRI scanning. It can be applied to the research of various experimental animal models, suitable for multiple experimental scenarios such as acute drug administration and drug delivery evaluation, and is especially suitable for the establishment of acute brain disease models and drug treatment research, and is easy to promote and use.

[0030] The technical solution of the present invention will be further described in detail below through the attached drawings and embodiments. Description of the Drawings

[0031] Figure 1 is a schematic structural diagram of an embodiment of a magnetic-compatible rodent brain injection device of the present invention;

[0032] Figure 2 is an exploded view of an embodiment of a magnetic-compatible rodent brain injection device of the present invention;

[0033] Figure 3 is an MRI scan diagram of an embodiment of a magnetic-compatible rodent brain injection method of the present invention, where (a) is before injecting the liquid and (b) is after injecting the liquid.

[0034] Reference Signs

[0035] 1. Fixed base; 2. Fixed notch; 3. X-axis track; 4. Y-axis track; 5. Threaded knob; 6. Fixing component; 7. Administration sleeve; 8. Positioning catheter; 9. Tightening knob; 10. Anti-slip pattern. Detailed implementation mode

[0036] The technical solution of the present invention will be further described below with reference to the drawings and embodiments.

[0037] Unless otherwise defined, the technical terms or scientific terms used in the present invention should have the ordinary meaning understood by those with ordinary skills in the field to which the present invention belongs.

[0038] Embodiment 1

[0039] As Figure 1 and Figure 2 shown, a magnetic compatibility rodent brain injection device includes a fixed base 1, a stereotactic fine adjustment system, an injection catheter system, and a drug delivery system (not marked in the figure). Both the fixed base 1 and the stereotactic fine adjustment system are made of non-metallic materials, specifically one of polyether ether ketone or Teflon, which are magnetic compatibility materials and also have high-performance biocompatible materials to avoid MRI artifacts and signal interference. The bottom surface of the fixed base 1 is fixedly connected to the surface of the skull through a cementing agent, and the cementing agent is dental cement or bone cement. The bottom surface of the fixed base 1 is provided with a number of fixed notches 2, which cooperate with the dental cement or bone cement to ensure that the device is firmly fixed on the animal skull, is compatible with the stereotactic instrument, and provides stable support.

[0040] The top surface of the fixed base 1 is provided with a stereotactic fine adjustment system, and the stereotactic fine adjustment system is fixedly connected to the outer ring of the injection catheter system. The stereotactic fine adjustment system is used to precisely adjust the position of the injection catheter system to ensure that the injection catheter system is accurately docked with the pre-drilled hole in the skull. Specifically, the stereotactic fine adjustment system includes a fine adjustment component and a fixing component 6. The fine adjustment component includes an X-axis track 3 and a Y-axis track 4. Both the X-axis track 3 and the Y-axis track 4 are provided with threaded knobs 5. The bottom surface of the X-axis track 3 is slidably connected to the fixed base 1 through the threaded knob 5, and the bottom surface of the Y-axis track 4 is slidably connected to the top surface of the X-axis track 3 through the threaded knob 5. The fixing component 6 is arranged at the center position of the top surface of the Y-axis track 4. The resolution of the threaded knob 5 reaches 0.1 mm. After the fine adjustment is completed, the device can be locked and the position of the injection catheter system can be fixed to prevent errors caused by the sliding of the device.

[0041] One end of the injection catheter system sequentially penetrates through the stereotactic fine-tuning system and the fixed base 1 and is connected to the interior of the skull. Specifically, the injection catheter system includes a drug delivery cannula 7 and a positioning catheter 8 sleeved on the outer wall of the drug delivery cannula 7. One end of the positioning catheter 8 is fixedly connected to the fixing assembly 6, and the other end of the positioning catheter 8 is provided with a fastening knob 9. The drug delivery cannula 7 is fixedly connected to the positioning catheter 8 through the fastening knob 9. The outer wall of the fastening knob 9 is provided with anti-slip threads 10, which facilitates quickly adjusting the position between the drug delivery cannula 7 and the positioning catheter 8, guiding the drug delivery cannula 7 to accurately reach the target area, and reducing the risk of brain tissue damage. The outer diameter of the drug delivery cannula 7 is equal to the inner diameter of the positioning catheter 8 to prevent the drug delivery cannula 7 from swinging. The length of the drug delivery cannula 7 is greater than the length of the positioning catheter 8. The positioning catheter 8 has an appropriate length and can provide a guiding function for the drug delivery cannula 7. The drug delivery cannula 7 can be fixed to it through the fastening knob 9. The positioning catheter 8 is located above the skull, avoiding direct contact with the brain, and effectively avoiding damage to the brain tissue.

[0042] The overall design of the fixed base 1, the positioning catheter 8, and the drug delivery cannula 7 ensures the imaging effect, the accuracy of the injection site, and the experimental reliability in a high-field MRI environment. The stereotactic fine-tuning system and the fastening knob 9 ensure the precise adjustment and positioning functions of the drug delivery cannula 7 in the X, Y, and Z three-dimensional directions, ensuring the precise docking of the positioning catheter 8 and the drug delivery cannula 7 to the target area and realizing high-precision position adjustment.

[0043] The other end of the injection catheter system is connected to the drug delivery system. The drug delivery system includes a PE tube, a micro syringe, and a micro infusion pump. The micro syringe adopts a threaded knob propulsion design to guide the liquid in the drug delivery cannula 7 to accurately reach the target area and ensure that the injection range is controllable. The micro infusion pump can precisely control the drug delivery rate and the injection volume. The micro syringe and the micro infusion pump can perform precise drug delivery control outside the magnetic resonance environment.

[0044] The drug delivery cannula 7 is made of quartz material, and the positioning catheter 8 and the fastening knob 9 are both made of non-metallic materials. Utilizing the biocompatibility and corrosion resistance of the quartz material, it is adapted to the micro infusion pump. The drug delivery cannula 7 is connected to the PE tube and filled with drugs or liquids to achieve high-precision drug delivery. The drug delivery cannula 7 has a certain length and hardness and can effectively reach the target area. After the experiment, the drug delivery cannula 7 can be safely removed without causing continuous harm to the animal.

[0045] Example 2

[0046] A magnetic-compatible rodent brain injection method (single-step injection method), using the device of Example 1, for mouse brain parenchyma drug injection experiments (mouse intracerebral hemorrhage model autologous blood injection experiment), to verify the effectiveness and operability of the present invention in precise autologous blood injection in the mouse brain parenchyma area, and at the same time evaluate its imaging compatibility in a high-field MRI environment.

[0047] Animal preparation: C57BL / 6 male mice were anesthetized by inhaling isoflurane at a concentration of 1.2% - 2.0%. The depth of anesthesia was moderate to avoid death caused by over-anesthesia or surgical interference caused by insufficient anesthesia.

[0048] The specific steps are as follows:

[0049] 1. Fixation and positioning

[0050] The mice were fixed in the prone position on a small animal stereotaxic apparatus, ensuring that the ear bars were firmly fixed and the head surface was parallel to the horizontal plane of the stereotaxic apparatus. The scalp of the mice was opened using surgical tools to expose the skull. A cranial drill was used to drill a hole in the skull above the target area of the right cerebral parenchyma, reaching the surface of the dura mater, avoiding damage to the brain tissue. (The target area for positioning was the striatum, 0.8 mm anterior to the bregma, 2.2 mm to the right, and 3.5 mm in depth).

[0051] 2. Installation of fixation base 1 and positioning catheter 8

[0052] ① Installation of fixation base 1: The fixation base 1 was bonded to the skull around the drilled hole using bone cement or dental cement and waited for curing to ensure stability.

[0053] ② Installation of positioning catheter 8: The positioning catheter 8 (outer diameter 0.4 mm, inner diameter 0.25 mm) was installed and locked on the stereotaxic fine adjustment system. The XY directions were finely adjusted through a threaded knob to make the positioning catheter 8 opposite to the drilled hole, and an optical fiber with an outer diameter of 0.25 mm was inserted to confirm alignment.

[0054] 3. Preparation of drug delivery cannula 7

[0055] The drug delivery cannula 7 made of quartz was connected to a PE10 tube. The PE10 tube passed through the inside and outside of the nuclear magnetic resonance (MRI) environment. The external PE10 tube was connected to a Hamilton microsyringe and fixed on a micro-injection pump. The PE10 tube was filled with physiological saline to prevent volume errors during injection due to gas compression. The drug delivery cannula 7 was pre-filled with the required dose of injection liquid.

[0056] 4. Blood collection from mice

[0057] Blood was collected from the tail artery of the mice: After local disinfection, the end of the mouse's tail was cut open with a small knife, and the naturally flowing blood was collected, avoiding squeezing to prevent contamination with tissue fluid. Using the previously prepared drug delivery cannula 7, 1 μL of air was first drawn from the external microsyringe to separate the physiological saline from the blood, and then 30 μL of blood was quickly drawn to prevent blood coagulation. After collection, it was placed beside the MRI bed for standby.

[0058] 5. Animal MRI scanning

[0059] Transfer the mice that have completed the surgical treatment to the MRI scanning workshop and perform imaging using a 9.4T small animal MRI scanner. Fix them using ear bars and dental bars to reduce head movement during scanning. In this embodiment, a 3-channel phased surface array optogenetic coil for mice is used, so that there is an operable space above the mouse's head, and the drug delivery cannula 7 can be placed. After fixing the coil, the physiological state of the mice is monitored and recorded in real time through a rectal temperature and respiratory monitor, and the normal physiological indicators are maintained by a water bed and an anesthesia machine.

[0060] 6. Place the drug delivery cannula 7

[0061] Pass the drug delivery cannula 7 filled with 30 μL of blood and prepared through the coil and insert it into the positioning catheter 8. Since the hardness of the quartz material is sufficient, the drug delivery cannula 7 can directly pass through the hole on the surface of the skull, pierce the dura mater and enter the brain. Measure the downward depth with a ruler, slowly insert the drug delivery cannula 7 deeper. After reaching the target depth, use the fastening knob 9 to fix the drug delivery cannula 7 and the positioning catheter 8. After preparation, perform magnetic resonance imaging on the mice.

[0062] 7. Check whether the target area is accurately positioned through magnetic resonance structural image scanning

[0063] Scan the T2-weighted structural image (TR / TE: 3000 / 33 ms, Matrix: 200×200, FOV: 20×20 mm2, averages: 2, slice thickness: 0.5 mm, 31 slices) to quickly locate the specific depth of the drug delivery cannula 7. The insertion depth of the drug delivery cannula 7 can be determined in real time through the magnetic resonance scan image. If the target brain area is not reached, repeat the steps of placing the drug delivery cannula 7, adjust the depth of the drug delivery cannula 7, and then perform a quick scan to ensure the accuracy of the injection positioning.

[0064] 8. Drug injection

[0065] After the drug delivery cannula 7 is adjusted to the target area (depth 3.5 mm) and confirmed to be correct, use a micro-injection pump at the preset time point according to the experimental protocol to slowly inject autologous blood of the mice at a rate of 2 μL / min (total injection volume 20 μL). After the injection is completed, continue to use MRI for imaging to check the distribution of the liquid in the brain parenchyma and verify the accuracy of the injection position.

[0066] 9. Remove the injection catheter system

[0067] Since the drug delivery cannula 7 is only fixed to the positioning catheter 8, the drug delivery cannula 7 can be removed after the scanning experiment is completed. If subsequent experimental injections are required at the same position, a protective cover can be installed to cover the positioning catheter 8 at the drilling position. The protective cover has the functions of sealing and repeated positioning, and supports safe operation and high repeatability in multiple injection experiments. If no further experiments are to be carried out, the positioning catheter 8 can be removed and the wound can be sealed to prevent infection.

[0068] Experimental results:

[0069] As Figure 3 shown, the target brain region (striatum) can be accurately located by the magnetically compatible brain parenchyma injection device, and the MRI imaging is clear. No artifacts or signal interference are observed, indicating that the device is fully compatible in a high-field MRI environment.

[0070] Example 3

[0071] A magnetically compatible rodent brain injection method (two-step injection method), using the device of Example 1, for mouse brain parenchyma drug injection experiments (autologous blood injection protocol for mouse intracerebral hemorrhage model).

[0072] There may be defects such as unstable hematoma volume or blood reflux in the single-step injection method for the intracerebral hemorrhage model. Therefore, the two-step injection method can be further used to improve the success rate and reduce blood reflux.

[0073] Modeling method: After the drug delivery cannula 7 reaches the target brain region through structural imaging scanning, first inject 10 μL of autologous blood, and then let it stand for 7 minutes to form a blood clot to block the needle track. During this period, enter the NMR scanning workroom to adjust the depth of the drug delivery cannula 7, lift the drug delivery cannula 7 by 2 mm and then fix it with the tightening knob 9. After completion, inject the remaining 10 μL of blood, which can effectively reduce the amount of blood reflux in the needle track and better control the size, shape and location of the hematoma.

[0074] Therefore, the present invention adopts the above-mentioned magnetically compatible rodent brain injection device and method, avoids signal artifacts or interference, has both imaging compatibility and operation convenience, realizes high-precision, good-repeatability, and small-brain tissue-damage brain drug injection, successfully realizes liquid distribution monitoring and position calibration, and provides a reliable technical means for brain science, drug research and development, and neurological disease research.

[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that they can still modify or equivalently replace the technical solutions of the present invention, and these modifications or equivalent replacements cannot make the modified technical solutions deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A magnetically compatible rodent brain injection device, characterized in that: It includes a fixed base, an injection catheter system and a drug delivery system. The bottom surface of the fixed base is fixedly connected to the surface of the skull through a cement. The top surface of the fixed base is provided with a stereotactic fine-tuning system, which is fixedly connected to the outer ring of the injection catheter system. One end of the injection catheter system sequentially penetrates through the stereotactic fine-tuning system and the fixed base and communicates with the interior of the skull. The other end of the injection catheter system communicates with the drug delivery system; The stereotactic fine-tuning system includes a fine-tuning component and a fixing component. The fine-tuning component includes an X-axis track and a Y-axis track. Both the X-axis track and the Y-axis track are provided with threaded knobs. The bottom surface of the X-axis track is slidably connected to the fixed base through the threaded knob. The bottom surface of the Y-axis track is slidably connected to the top surface of the X-axis track through the threaded knob. The fixing component is arranged at the center position of the top surface of the Y-axis track; The injection catheter system includes a drug delivery cannula and a positioning catheter sleeved on the outer wall of the drug delivery cannula. One end of the positioning catheter is fixedly connected to the fixing component. The other end of the positioning catheter is provided with a fastening knob. The drug delivery cannula is fixedly connected to the positioning catheter through the fastening knob. The outer wall of the fastening knob is provided with anti-slip lines; Use a mouse 3-channel phased surface array optogenetic coil to provide an operable space on the top of the mouse's head so that the drug delivery cannula can be inserted. After the coil is fixed, the physiological state of the mouse is monitored and recorded in real time by a rectal temperature and respiratory monitor; The drug delivery cannula is made of quartz, and the positioning catheter and the fastening knob are both made of non-metallic materials; Both the fixed base and the stereotactic fine-tuning system are made of non-metallic materials.

2. The magnetic compatibility rodent brain injection device according to claim 1, wherein: The bottom surface of the fixed base is provided with a plurality of fixing notches, and the cement is one or both of dental cement or bone cement.

3. A magnetically compatible rodent brain injection device according to claim 1, wherein: The outer diameter of the drug delivery cannula is equal to the inner diameter of the positioning catheter, and the length of the drug delivery cannula is greater than the length of the positioning catheter.

4. A magnetic compatibility rodent brain injection device according to claim 1, characterized in that: The materials used for the fixed base and the stereotactic fine-tuning system are one of polyether ether ketone or polytetrafluoroethylene.

5. A magnetic compatibility rodent brain injection device according to claim 1, characterized in that: The drug delivery system includes a PE tube, a micro syringe and a micro infusion pump. The micro syringe adopts a threaded knob propulsion design.

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