Ganoderma spore fermentation product, preparation method and application of ganoderma spore fermentation product in cosmetics

Abstract

The invention provides a ganoderma spore fermentation product, a preparation method and application of the ganoderma spore fermentation product in cosmetics, and belongs to the technical field of microbial fermentation. The ganoderma spore fermentation product is prepared through fermentation of torulopsis nibea, the torulopsis nibea is torulopsis nibea CICC 1706, a fermentation medium contains ganoderma spore powder and glucose, and the ganoderma spore fermentation product contains triterpenes and sophorolipid. The ganoderma lucidum spore oil is promoted to be slowly released in the fermentation process through the metabolism effect of the torulopsis bees; meanwhile, part of grease reacts with sugar in the culture medium to generate sophorolipid, so that a fermentation product forms a system similar to emulsion, and efficient extraction of the ganoderma lucidum spore oil is realized.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a ganoderma lucidum spore fermentation product, a preparation method and application thereof in cosmetics. Background Art

[0002] Ganoderma lucidum spore oil is an oily liquid obtained by breaking the spores of Ganoderma lucidum and extracting them. It contains fatty acids, sterols, terpenes and other ingredients, especially the active ingredients of Ganoderma lucidum triterpenes, which have health functions such as anti-aging, anti-tumor, and immune enhancement, and have been widely used in health products. In recent years, the application of Ganoderma lucidum spore oil in the field of cosmetics and skin care has gradually emerged. Its efficacy in anti-aging and skin repair has been recognized by consumers, showing significant skin care value.

[0003] There are two main extraction methods for Ganoderma lucidum spore oil: organic solvent extraction and supercritical carbon dioxide extraction. The organic solvent extraction method is relatively mature, but it usually requires the use of organic solvents such as volatile alcohols and petroleum ether, and there are problems such as high energy consumption, high solvent usage, solvent residues and safe production. In addition, the traditional organic solvent extraction method may cause damage to the heat-sensitive components in Ganoderma lucidum spore oil, affecting its activity. The supercritical carbon dioxide extraction method has gradually attracted attention due to its advantages such as environmental protection, low energy consumption, and low-temperature extraction. This method can be carried out at a lower temperature, avoiding the degradation of heat-sensitive components, and at the same time, no organic solvent is used, avoiding the problem of solvent residue. However, the supercritical carbon dioxide extraction equipment is relatively expensive, and in practical applications, a small amount of organic solvent still needs to be added as an "entrainer" to enhance the extraction efficiency, which may lead to the risk of solvent residues, for example, the methods disclosed in patent documents CN104073344B or CN103301174B.

[0004] Nowadays, water is the main solvent for many cosmetics. If oily ingredients are used, surfactants are usually added to help dissolve and emulsify. However, the surfactants in most cosmetics are chemically synthesized or semi-synthetic ingredients. In addition, most cosmetics manufacturers also have high requirements for the stability of raw materials.

[0005] In view of the above problems, it is particularly urgent to develop a raw material containing Ganoderma lucidum spore oil that is more suitable for use in cosmetics. Summary of the invention

[0006] In view of this, the technical problem to be solved by the present invention is to provide a method for preparing a fermentation product of Ganoderma lucidum spores, wherein the Ganoderma lucidum spore oil is slowly released during the fermentation process through the metabolic action of Torulopsis mellifera; at the same time, Torulopsis mellifera utilizes part of the oil in the Ganoderma lucidum spores and the sugar in the culture medium to synthesize sophorolipids, so that the fermentation product forms an emulsion-like system, thereby achieving efficient extraction of Ganoderma lucidum spore oil.

[0007] In one aspect, the present invention provides a Ganoderma lucidum spore fermentation product, which is obtained by fermenting Torulopsis apis, wherein the Torulopsis apis is Torulopsis apis. Torulopsis apicola CICC 1706, the fermentation medium contains ganoderma lucidum spore powder and glucose, and the ganoderma lucidum spore fermentation product contains triterpenes and sophorolipids.

[0008] In another aspect, the present invention provides a method for preparing a Ganoderma lucidum spore fermentation product, comprising: Torulopsis apis Torulopsis apicola CICC 1706 is the strain, The fermentation is carried out in a fermentation medium containing ganoderma lucidum spore powder and glucose to obtain a ganoderma lucidum spore fermentation product.

[0009] Optionally, the fermentation medium contains 125-140 g / L broken wall Ganoderma lucidum spore powder, 40-45 g / L glucose, 2.5 g / L yeast extract powder, 4 g / L corn steep liquor, natural pH, and sterilized at 105° C. for 20 min.

[0010] Optionally, the fermentation process parameters include: inoculating the seed liquid of the honey bee Torulopsis yeast into the culture medium, fermenting and culturing at 32-33°C for 60-72h, then reducing the temperature to 27°C and continuing to ferment for 24-30h, stirring at 400-480rpm, controlling the dissolved oxygen content to be greater than 40%, and obtaining a fermentation liquid.

[0011] The research of the present invention shows that sophorolipids are more produced in the stable period (late period) of fermentation. Appropriately lowering the temperature can reduce the reproduction of the bacteria and prolong the stable period, which is conducive to moderately increasing the production of sophorolipids and meeting the application requirements of the Ganoderma lucidum spore fermentation product of the present invention.

[0012] Optionally, the seed liquid of the Torulopsis apis is prepared by the following method: after activating the Torulopsis apis strain, inoculate it into a seed liquid culture medium, and culture it aerobically at 33-34° C. for 32-36 hours to obtain the seed liquid.

[0013] Optionally, the seed liquid culture medium contains 10 g / L yeast extract, 20 g / L peptone, 50 g / L glucose, 6 g / L olive oil, natural pH, and is sterilized at 115° C. for 15 min.

[0014] Optionally, the method for preparing a Ganoderma lucidum spore fermentation product further includes: a post-treatment step, which includes: heating the fermentation liquid to 60° C. and maintaining it for 40 minutes to inactivate bacteria, stopping fermentation, centrifuging, filtering with filter cloth and microfiltration in sequence to obtain the Ganoderma lucidum spore fermentation product.

[0015] Optionally, the filter cloth filtration uses an 800-mesh filter cloth, and the microfiltration uses a 0.1 or 0.22 μm filter.

[0016] On the other hand, the present invention also provides the use of the fermentation product of Ganoderma lucidum spores in cosmetics.

[0017] Compared with the prior art, the present invention has the following beneficial effects: The present invention screens and uses Torulopsis apis Torulopsis apicola CICC 1706, this strain adapts to special fermentation needs, can efficiently metabolize the oil in Ganoderma lucidum spores, and can produce a certain amount of sophorolipids to improve the stability of the fermentation liquid.

[0018] The fermentation process of the present invention adopts a completely organic culture medium to replace the commonly used inorganic salts, which is more in line with the use scenarios and consumption concepts of cosmetics.

[0019] The invention ferments the ganoderma lucidum spore powder by using the bee spore yeast, so that the ganoderma lucidum spore oil is slowly released during the fermentation process; at the same time, part of the oil reacts with the sugar in the culture medium to generate sugar sophorolipids, which act as surfactants and slowly form an emulsified system with other oily components, so that no additional surfactants are needed for solubilization and emulsification, thereby realizing efficient extraction of the ganoderma lucidum spore oil, and providing a new technical path for the green and efficient preparation of the ganoderma lucidum spore oil. The ganoderma lucidum spore fermentation product of the invention has more excellent anti-oxidation, anti-aging and repairing effects, and has good application prospects in cosmetics. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 : Cell migration assay results. DETAILED DESCRIPTION

[0022] In order to better understand the present invention, the content of the present invention is further clearly set forth in conjunction with the embodiments below, but the protection content of the present invention is not limited to the following embodiments. In the following description, a large number of specific details are provided in order to provide a more thorough understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details.

[0023] The honey bee yeast of the present invention Torulopsis apicola CICC 1706, Bombyx mori Candida bombicola ATCC 22214, the depository is China Industrial Microbiological Culture Collection Center; the brewer's yeast Saccharomyces cerevisiae CCTCC M2016509, the depository is China Center for Type Culture Collection. The above strains were purchased in the form of freeze-dried powder.

[0024] The ganoderma lucidum spore powder of the present invention is passed through a 100-mesh sieve and is purchased.

[0025] Example 1: A method for preparing a Ganoderma lucidum spore fermentation product, comprising the following steps: Torulopsis apis Torulopsis apicola After the CICC 1706 strain was activated on the plate, it was inoculated into the seed liquid culture medium and cultured at 34°C and 220 rpm for 32 h to obtain the seed liquid; wherein: the seed liquid culture medium (L -1 ): 10g yeast extract, 20g peptone, 50g glucose, 6g olive oil, natural pH, sterilized at 115℃ for 15min; 100 mL of seed liquid was inoculated into 4 L of fermentation medium, and fermented at 32 °C for 72 h, then cooled to 27 °C and continued to ferment for 30 h, with a stirring speed of 450 rpm and dissolved oxygen content controlled to be >40%; wherein: fermentation medium (L -1 ): 130g Ganoderma lucidum spore powder, 40g glucose, 2.5g yeast extract powder, 4g corn steep liquor, natural pH, sterilized at 105℃ for 20min; The temperature was raised to 60°C and maintained for 40 minutes to inactivate the bacteria, and the fermentation was stopped. The fermentation liquid was first centrifuged to remove most of the solids, and then filtered with an 800-mesh filter cloth and a 0.1 μm filter to obtain the Ganoderma lucidum spore fermentation product.

[0026] Example 2: A method for preparing a Ganoderma lucidum spore fermentation product, comprising the following steps: Torulopsis apis Torulopsis apicola After the CICC 1706 strain was activated on the plate, it was inoculated into the seed liquid culture medium and cultured at 34°C and 220 rpm for 36 h to obtain the seed liquid; wherein: the seed liquid culture medium (L -1 ): 10g yeast extract, 20g peptone, 50g glucose, 6g olive oil, natural pH, sterilized at 115℃ for 15min; 600 mL of seed liquid was inoculated into 30 L of fermentation medium, and the culture was first fermented at 33 °C for 63 h, then the temperature was lowered to 27 °C and the fermentation was continued for 24 h. The stirring speed was 400 rpm, and the dissolved oxygen content was controlled to be > 40%; wherein: the fermentation medium (L -1 ): 140g broken wall Ganoderma lucidum spore powder, 45g glucose, 2.5g yeast extract powder, 4g corn steep liquor, natural pH, sterilized at 105℃ for 20min; The temperature was raised to 60°C and maintained for 40 minutes to inactivate the bacteria, and the fermentation was stopped. The fermentation liquid was first centrifuged to remove most of the solids, and then filtered with an 800-mesh filter cloth and a 0.22 μm filter to obtain the Ganoderma lucidum spore fermentation product.

[0027] Example 3: A method for preparing a Ganoderma lucidum spore fermentation product, comprising the following steps: Torulopsis apis Torulopsis apicola After the CICC 1706 strain was activated on the plate, it was inoculated into the seed liquid culture medium and cultured at 33°C and 220 rpm for 35 h to obtain the seed liquid; wherein: the seed liquid culture medium (L -1 ): 10g yeast extract, 20g peptone, 50g glucose, 6g olive oil, natural pH, sterilized at 115℃ for 15min; 115mL of seed liquid was inoculated into 5L of fermentation medium, and fermented at 32.5℃ for 60h, then cooled to 27℃ and continued to ferment for 27h, with stirring speed of 480rpm and dissolved oxygen content controlled to be >40%; wherein: fermentation medium (L -1 ): 125g Ganoderma lucidum spore powder, 42g glucose, 2.5g yeast extract powder, 4g corn steep liquor, natural pH, sterilized at 105℃ for 20min; The temperature was raised to 60°C and maintained for 40 minutes to inactivate the bacteria, and the fermentation was stopped. The fermentation liquid was first centrifuged to remove most of the solids, and then filtered with an 800-mesh filter cloth and a 0.22 μm filter to obtain the Ganoderma lucidum spore fermentation product.

[0028] Control Example 1: Control Example 1 provides a Ganoderma lucidum spore oil and spore oil emulsion extracted by an organic solvent, the method is as follows: 130g of Ganoderma lucidum spore powder, add 1000mL of n-hexane, ultrasonicate for 30min at room temperature (25°C), shake, let stand, filter to obtain the filtrate, evaporate to dryness under reduced pressure, and obtain Ganoderma lucidum spore oil.

[0029] Control Example 2: Control Example 2 provides a Ganoderma lucidum spore oil and spore oil emulsion extracted by supercritical CO2 method. The method is as follows: take Ganoderma lucidum spore powder, granulate it, pass it through a 60-mesh sieve, weigh 130g of Ganoderma lucidum spore granules and fill them into a 500mL supercritical CO2 extraction kettle. The extraction conditions are 30MPa, 45℃, 3h, and collect Ganoderma lucidum spore oil.

[0030] Comparative Example 3: Compared with Example 1, only the strain was replaced by: Candida bombus Candida bombicola ATCC22214, and the other process conditions remained unchanged.

[0031] Comparative Example 4: Compared with Example 1, only the strain was replaced by: Saccharomyces cerevisiae Saccharomyces cerevisiae CCTCC M2016509, and the other process conditions remain unchanged.

[0032] Reference Example 5: Reference Example 5 provides a method for extracting spore powder using a biosurfactant sophorolipid to obtain a spore oil solution, comprising: S1. Dissolve 40 g of sophorolipid in 1960 g of pure water as an extract; S2, taking 260g of broken spore powder of Ganoderma lucidum, and mixing it with the above extract; S3, heating and stirring, raising the temperature to 80°C, maintaining for 3h; S4, stop heating, centrifuge the mixed solution, and take the supernatant; S5. The supernatant is filtered using qualitative filter paper and 0.22 μm filter membrane in turn.

[0033] The following tests and analyses were performed on the above-mentioned Examples 1-2 and Comparative Examples 1-5.

[0034] 1. Physical and chemical performance testing The solid residues obtained by centrifugation after fermentation in Examples 1 and 2 and Control Examples 3 and 4 (only the dark brown part with uniform color) were collected, and the solids obtained by centrifugation of the extract in Control Example 5 were collected, dried at 65°C and stored for the detection of the oil content of the residues.

[0035] Take the products of Examples 1 and 2, and Reference Examples 3 and 5, respectively, use hydrochloric acid to adjust the pH to less than 2, thaw after freezing, let stand for 24 hours, separate the solutions, and collect the oily substance in the upper layer for the detection of triterpene content and peroxide value. The product of Reference Example 4 cannot be collected to detect the triterpene content and peroxide value due to too little oil content.

[0036] 1.1. Detection of oil content in residue With reference to the test method of "GB / T 14488.1-2008 Oil content of vegetable oils", the oil content in the residue after extraction and fermentation of Ganoderma lucidum spore powder was determined, and the test results are shown in Table 1. The lower the oil content in the residue, the more efficient the corresponding extraction or fermentation method is, and the more fully the Ganoderma lucidum spore oil can be extracted.

[0037] Table 1 Detection results of residue oil content The results in Table 1 show that the oil content in the residue obtained by the fermentation method of Examples 1 and 2 of the present invention is less than 4%. Compared with Control Examples 3 and 4, the fermentation method of the present invention is significantly more efficient in extracting Ganoderma lucidum spore oil from Ganoderma lucidum spore powder and can more fully extract Ganoderma lucidum spore oil.

[0038] 1.2. Triterpene content and peroxide value detection The method described in the reference (Wang Hongxia, Zhao Zhenyu, Dong Xiaofen, et al. Study on the preparation process of Ganoderma lucidum spore oil [J]. China Food Additives, 2024, 35(1): 224-230) was used to detect the triterpenoid content of the oily component by the vanillin-perchloric acid method; the peroxide value was determined according to GB 5009.227-2016 National Food Safety Standard Determination of Peroxide Value in Food. The test results are shown in Table 2.

[0039] Table 2 Triterpene content and peroxide value test results The results in Table 2 show that the content of oily triterpenes in the products of Examples 1 and 2 of the present invention is significantly higher than that in the control example. This is because part of the fat in the Ganoderma lucidum spore oil in Examples 1 and 2 is converted into sophorolipids by the fermentation bacteria, which relatively increases the content of triterpenes. At the same time, the peroxide values ​​of the products of Examples 1 and 2 of the present invention are lower, indicating that the stability of the products obtained by the method of the present invention is better during storage and use.

[0040] 1.3. Sophorolipid content detection The method described in the reference (Shen Jing. Research on the synthesis of sophorolipids by using medium carbon chain length alkanes by Pseudomonas aeruginosa [D]. Shandong University, 2012.) was used to measure the sophorolipid content in the fermentation products of Examples 1 and 2 and Comparative Examples 3 and 4. The fermentation products of Ganoderma lucidum spores were first extracted with ethyl acetate and then detected and converted by the anthrone method. The test results are shown in Table 3.

[0041] Table 3 Sophorolipid content detection results The results in Table 3 show that the fermentation products of Examples 1 and 2 of the present invention have significantly more sophorolipids, while the fermentation products of Control Examples 3 and 4 have relatively lower sophorolipid contents, especially Control Example 4 can be considered to produce no sophorolipids. This result also explains the intrinsic reason why Examples 1 and 2 of the present invention can efficiently transfer oil from Ganoderma lucidum spore powder.

[0042] 2. Efficacy testing Since the products obtained in Examples 1 and 2 are pure oily components, in order to facilitate the comparison of Examples 1 and 2 with other control examples and to conduct cell tests, the products of Control Examples 1 and 2 are respectively processed as follows, and the specific steps are as follows: S1. Dissolve 0.1g xanthan gum in 94.5g pure water, stir to dissolve and slowly heat to 85℃ to keep warm; S2, take 3.4g of the product of Reference Example 1 or 2, mix with 2g of sophorolipid, and heat to 85°C for insulation; The mixture of steps S3 and S2 is added to the solution of step S1 and homogenized by a homogenizer until an emulsion is formed.

[0043] 2.1 Free radical scavenging test The test method was in accordance with "T / SHRH 006-2018 Cosmetics - Free Radical (DPPH) Scavenging Test Method", and the DPPH free radical scavenging rate was tested by diluting with pure water to 0.5%, 1%, 2% and 4% concentrations. Control Examples 1 and 2 were emulsified according to the above-mentioned treatment steps to form an emulsion and then diluted. The test results are shown in Table 4.

[0044] Table 4 Free radical scavenging rate test results The results in Table 4 show that at the same dilution concentration, the free radical scavenging rates of the products of Examples 1 and 2 of the present invention are more significant, indicating that the antioxidant properties of the products of Examples 1 and 2 of the present invention are more excellent.

[0045] 2.2 Cell migration assay The test method was based on the method described in the literature (Liang CC, Park AY, Guan J L. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro [J]. Nature Protocols, 2007, 2 (2): 329-333.) to detect the 24-hour cell scratch repair rate. Control Examples 1 and 2 used emulsified microbial fermentation biosurfactant to form an emulsion and then diluted it to a concentration of 0.5%. At this concentration, all test samples were non-cytotoxic. The blank control group was saline. The test results are as follows: Figure 1 shown.

[0046] Figure 1 The results showed that the products of Examples 1 and 2 of the present invention could more significantly improve the cell migration rate, indicating that the products of Examples 1 and 2 have the potential to promote tissue repair and regeneration.

[0047] 3. Application testing Application example: The fermentation product of Example 1 is applied to a cosmetic to prepare a Ganoderma lucidum fermented anti-wrinkle moisturizing lotion, and the formulation is as follows: Table 5 Cosmetic formulation containing the fermentation product of Example 1 Twelve healthy female volunteers aged 33 to 54 years old with dry skin and fine lines around the eyes were recruited. The application samples were prepared according to the above formula, put into pump bottles, and given to volunteers for use. They were required to use it 3 times a day in the morning, noon and evening. When using, take 2 pumps and apply them evenly on the face for 14 consecutive days. Before use and after 14 days of use, relevant tests and evaluations were carried out. The Courage+Khazaka multi-probe skin test system (MPA6) was used to measure the transepidermal water loss rate TEWL and skin elasticity R2. A decrease in TEWL value indicates an improvement in skin barrier function (repair effect), and a high skin elasticity R2 index indicates good skin elasticity. The results are shown in Table 6.

[0048] Table 6 Human test results The results in Table 6 show that after applying the fermented product of Ganoderma lucidum spores of the present invention, the transepidermal water loss (TEWL) value of the skin is significantly reduced, indicating that the skin barrier function is significantly improved, and the fermented product of Ganoderma lucidum spores exhibits excellent repairing effect. At the same time, the skin elasticity R2 index is simultaneously increased, and the skin elasticity is significantly improved, further proving that the fermented product of the present invention has the effect of significantly improving skin elasticity.

[0049] In summary, the present invention ferments Ganoderma lucidum spore powder by Tocopherols of honey bees, thereby promoting the slow release of Ganoderma lucidum spore oil during the fermentation process; Tocopherols of honey bees utilizes the oil in part of Ganoderma lucidum spores and the sugar in the culture medium to synthesize sophorolipids, and sophorolipids serve as surfactants to slowly form an emulsified system with other oily components, thereby realizing solvent-free extraction of Ganoderma lucidum spore oil. The Ganoderma lucidum spore fermentation product obtained by the preparation method has more excellent antioxidant, anti-aging and repair effects, and has good application prospects in cosmetics.

[0050] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Other modifications or equivalent substitutions made to the technical solution of the present invention by ordinary technicians in the field should be included in the scope of the claims of the present invention as long as they do not depart from the spirit and scope of the technical solution of the present invention.

Claims

1. A ganoderma lucidum spore fermentation product, characterized in that: It is prepared by fermenting honey bee Torulopsis yeast, and the honey bee Torulopsis yeast is honey bee Torulopsis yeast Torulopsis apicola CICC 1706, the fermentation medium contains ganoderma lucidum spore powder and glucose, and the ganoderma lucidum spore fermentation product contains triterpenes and sophorolipids.

2. A method for preparing a Ganoderma lucidum spore fermentation product, characterized in that: Torulopsis apis Torulopsis apicola CICC 1706 is the strain, The fermentation is carried out in a fermentation medium containing ganoderma lucidum spore powder and glucose to obtain a ganoderma lucidum spore fermentation product.

3. The method for preparing a Ganoderma lucidum spore fermentation product as claimed in claim 2, characterized in that: The fermentation medium contains 125-140 g / L of broken wall ganoderma spore powder, 40-45 g / L of glucose, 2.5 g / L of yeast extract powder, 4 g / L of corn steep liquor, natural pH, and is sterilized at 105° C. for 20 minutes.

4. The method for preparing a Ganoderma lucidum spore fermentation product as claimed in claim 2, characterized in that: The fermentation process parameters include: inoculating the seed liquid of the honey bee Torulopsis yeast into the culture medium, fermenting and culturing at 32-33° C. for 60-72 hours, then reducing the temperature to 27° C. and continuing fermentation for 24-30 hours, stirring at 400-480 rpm, controlling the dissolved oxygen content to be greater than 40%, and obtaining a fermentation liquid.

5. The method for preparing a Ganoderma lucidum spore fermentation product as claimed in claim 4, characterized in that: The seed liquid of the Torulopsis apis yeast is prepared by the following method: after activating the Torulopsis apis yeast strain, inoculating it into a seed liquid culture medium, and culturing it aerobically at 33-34° C. for 32-36 hours to obtain the seed liquid.

6. The method for preparing a Ganoderma lucidum spore fermentation product according to claim 5, characterized in that: The seed liquid culture medium contains 10 g / L yeast extract, 20 g / L peptone, 50 g / L glucose, 6 g / L olive oil, has a natural pH, and is sterilized at 115° C. for 15 min.

7. The method for preparing a Ganoderma lucidum spore fermentation product as claimed in claim 4, characterized in that: Also includes: The post-processing step includes: heating the fermentation liquid to 60°C and maintaining it for 40 minutes to inactivate bacteria, stopping fermentation, centrifuging, filtering with filter cloth and microfiltration in sequence to obtain a Ganoderma lucidum spore fermentation product.

8. The method for preparing a Ganoderma lucidum spore fermentation product according to claim 7, characterized in that: The filter cloth filtration uses 800 mesh filter cloth, and the microfiltration uses 0.1 or 0.22 μm filter.

9. Use of the Ganoderma lucidum spore fermentation product according to claim 1 or the Ganoderma lucidum spore fermentation product obtained by the preparation method of the Ganoderma lucidum spore fermentation product according to any one of claims 2 to 8 in cosmetics, wherein the cosmetics have antioxidant, anti-aging and repairing effects.

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